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[PMID]:29287781
[Au] Autor:Cervantes-Llanos M; Lagumersindez-Denis N; Marín-Prida J; Pavón-Fuentes N; Falcon-Cama V; Piniella-Matamoros B; Camacho-Rodríguez H; Fernández-Massó JR; Valenzuela-Silva C; Raíces-Cruz I; Pentón-Arias E; Teixeira MM; Pentón-Rol G
[Ad] Endereço:Center for Genetic Engineering and Biotechnology (CIGB), Ave. 31 e/ 158 y 190, Cubanacán, Playa, Havana, PO Box 6162, Cuba.
[Ti] Título:Beneficial effects of oral administration of C-Phycocyanin and Phycocyanobilin in rodent models of experimental autoimmune encephalomyelitis.
[So] Source:Life Sci;194:130-138, 2018 Feb 01.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The only three oral treatments currently available for multiple sclerosis (MS) target the relapsing forms of the disease and concerns regarding efficacy, safety and tolerability limit their use. Identifying novel oral disease-modifying therapies for MS, targeting both its inflammatory and neurodegenerative components is still a major goal. AIM: The scope of this study was to provide evidence that the oral administration of C-Phycocyanin (C-PC), the main biliprotein of the Spirulina platensis cyanobacteria and its tetrapyrrolic prosthetic group, Phycocyanobilin (PCB), exert ameliorating actions on rodent models of experimental autoimmune encephalomyelitis (EAE). MAIN METHODS: EAE was induced in Lewis rats using the spinal cord encephalitogen from Sprague Dawley rats and in C57BL6 mice with MOG peptide. Clinical signs, motor function, oxidative stress markers, cytokine levels by ELISA and transmission electron microscopy analysis were assessed. KEY FINDINGS: Either prophylactic or early therapeutic administration of C-PC to Lewis rats with EAE, significantly improved clinical signs and restored the motor function of the animals. Furthermore, C-PC positively modulated oxidative stress markers measured in brain homogenate and serum and protected the integrity of cerebral myelin sheaths as shown by transmission electron microscopy analysis. In C57BL/6 mice with EAE, PCB orally improved clinical status of the animals and reduced the expression levels of brain IL-6 and IFN-γ proinflammatory cytokines. SIGNIFICANCE: These results, for the first time, support the fact that both C-PC and PCB administered orally could potentially improve neuroinflammation, protect from demyelination and axonal loss, which may be translated into an improved quality of life for MS patients.
[Mh] Termos MeSH primário: Encéfalo/efeitos dos fármacos
Encefalomielite Autoimune Experimental/tratamento farmacológico
Fármacos Neuroprotetores/uso terapêutico
Ficobilinas/uso terapêutico
Ficocianina/uso terapêutico
[Mh] Termos MeSH secundário: Administração Oral
Animais
Anti-Inflamatórios/administração & dosagem
Anti-Inflamatórios/química
Anti-Inflamatórios/uso terapêutico
Encéfalo/patologia
Citocinas/análise
Modelos Animais de Doenças
Encefalomielite Autoimune Experimental/patologia
Feminino
Interleucina-6/análise
Masculino
Camundongos Endogâmicos C57BL
Fármacos Neuroprotetores/administração & dosagem
Fármacos Neuroprotetores/química
Ficobilinas/administração & dosagem
Ficobilinas/química
Ficocianina/administração & dosagem
Ficocianina/química
Ratos Endogâmicos Lew
Ratos Sprague-Dawley
Spirulina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Cytokines); 0 (Interleukin-6); 0 (Neuroprotective Agents); 0 (Phycobilins); 11016-15-2 (Phycocyanin); 36NUT04V2K (phycocyanobilin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171231
[St] Status:MEDLINE


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[PMID]:28911120
[Au] Autor:Hochrein L; Machens F; Messerschmidt K; Mueller-Roeber B
[Ad] Endereço:University of Potsdam, Cell2Fab Research Unit, Karl-Liebknecht-Str. 24-25, 14476 Potsdam, Germany.
[Ti] Título:PhiReX: a programmable and red light-regulated protein expression switch for yeast.
[So] Source:Nucleic Acids Res;45(15):9193-9205, 2017 Sep 06.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Highly regulated induction systems enabling dose-dependent and reversible fine-tuning of protein expression output are beneficial for engineering complex biosynthetic pathways. To address this, we developed PhiReX, a novel red/far-red light-regulated protein expression system for use in Saccharomyces cerevisiae. PhiReX is based on the combination of a customizable synTALE DNA-binding domain, the VP64 activation domain and the light-sensitive dimerization of the photoreceptor PhyB and its interacting partner PIF3 from Arabidopsis thaliana. Robust gene expression and high protein levels are achieved by combining genome integrated red light-sensing components with an episomal high-copy reporter construct. The gene of interest as well as the synTALE DNA-binding domain can be easily exchanged, allowing the flexible regulation of any desired gene by targeting endogenous or heterologous promoter regions. To allow low-cost induction of gene expression for industrial fermentation processes, we engineered yeast to endogenously produce the chromophore required for the effective dimerization of PhyB and PIF3. Time course experiments demonstrate high-level induction over a period of at least 48 h.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/genética
Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética
Proteínas de Ligação a DNA/genética
Engenharia Genética/métodos
Proteínas de Homeodomínio/genética
Fitocromo B/genética
Saccharomyces cerevisiae/genética
[Mh] Termos MeSH secundário: Arabidopsis/química
Arabidopsis/genética
Arabidopsis/metabolismo
Proteínas de Arabidopsis/metabolismo
Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo
Proteínas de Ligação a DNA/metabolismo
Expressão Gênica
Vetores Genéticos/química
Vetores Genéticos/metabolismo
Proteínas de Fluorescência Verde/genética
Proteínas de Fluorescência Verde/metabolismo
Proteínas de Homeodomínio/metabolismo
Luz
Transdução de Sinal Luminoso
Ficobilinas/biossíntese
Ficobilinas/genética
Ficocianina/biossíntese
Ficocianina/genética
Fitocromo B/metabolismo
Plasmídeos/química
Plasmídeos/metabolismo
Regiões Promotoras Genéticas
Multimerização Proteica
Saccharomyces cerevisiae/metabolismo
Saccharomyces cerevisiae/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Basic Helix-Loop-Helix Transcription Factors); 0 (DNA-Binding Proteins); 0 (Homeodomain Proteins); 0 (PHYB protein, Arabidopsis); 0 (PIF3 protein, Arabidopsis); 0 (Phycobilins); 0 (enhanced green fluorescent protein); 11016-15-2 (Phycocyanin); 136250-22-1 (Phytochrome B); 147336-22-9 (Green Fluorescent Proteins); 36NUT04V2K (phycocyanobilin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx610


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[PMID]:28371229
[Au] Autor:Mackey KRM; Post AF; McIlvin MR; Saito MA
[Ad] Endereço:Earth System Science, University of California Irvine, Irvine, CA, 92697, USA.
[Ti] Título:Physiological and proteomic characterization of light adaptations in marine Synechococcus.
[So] Source:Environ Microbiol;19(6):2348-2365, 2017 Jun.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Marine Synechococcus thrive over a range of light regimes in the ocean. We examined the proteomic, genomic and physiological responses of seven Synechococcus isolates to moderate irradiances (5-80 µE m s ), and show that Synechococcus spans a continuum of light responses ranging from low light optimized (LLO) to high light optimized (HLO). These light responses are linked to phylogeny and pigmentation. Marine sub-cluster 5.1A isolates with higher phycouribilin: phycoerythrobilin ratios fell toward the LLO end of the continuum, while sub-cluster 5.1B, 5.2 and estuarine Synechococcus with less phycouribilin fell toward the HLO end of the continuum. Global proteomes were highly responsive to light, with > 50% of abundant proteins varying more than twofold between the lowest and highest irradiance. All strains downregulated phycobilisome proteins with increasing irradiance. Regulation of proteins involved in photosynthetic electron transport, carbon fixation, oxidative stress protection (superoxide dismutases) and iron and nitrogen metabolism varied among strains, as did the number of high light inducible protein (Hlip) and DNA photolyase genes in their genomes. All but one LLO strain possessed the photoprotective orange carotenoid protein (OCP). The unique combinations of light responses in each strain gives rise to distinct photophysiological phenotypes that may affect Synechococcus distributions in the ocean.
[Mh] Termos MeSH primário: Transporte de Elétrons/genética
Fotossíntese/genética
Ficobilinas/metabolismo
Ficoeritrina/metabolismo
Synechococcus/genética
Synechococcus/fisiologia
Urobilina/análogos & derivados
[Mh] Termos MeSH secundário: Adaptação Ocular
Ciclo do Carbono/genética
Luz
Estresse Oxidativo/genética
Ficobilissomas/metabolismo
Filogenia
Proteômica
Synechococcus/isolamento & purificação
Urobilina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phycobilins); 0 (Phycobilisomes); 11016-17-4 (Phycoerythrin); 18097-67-1 (phycoerythrobilin); 1856-98-0 (Urobilin); 61932-71-6 (phycourobilin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.13744


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[PMID]:28073912
[Au] Autor:Gasper R; Schwach J; Hartmann J; Holtkamp A; Wiethaus J; Riedel N; Hofmann E; Frankenberg-Dinkel N
[Ad] Endereço:From the Protein Crystallography Group and.
[Ti] Título:Distinct Features of Cyanophage-encoded T-type Phycobiliprotein Lyase ΦCpeT: THE ROLE OF AUXILIARY METABOLIC GENES.
[So] Source:J Biol Chem;292(8):3089-3098, 2017 Feb 24.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Auxiliary metabolic genes (AMG) are commonly found in the genomes of phages that infect cyanobacteria and increase the fitness of the cyanophage. AMGs are often homologs of host genes, and also typically related to photosynthesis. For example, the Φ gene in the cyanophage P-HM1 encodes a putative phycobiliprotein lyase related to cyanobacterial T-type lyases, which facilitate attachment of linear tetrapyrrole chromophores to Cys-155 of phycobiliprotein ß-subunits, suggesting that ΦCpeT may also help assemble light-harvesting phycobiliproteins during infection. To investigate this possibility, we structurally and biochemically characterized recombinant ΦCpeT. The solved crystal structure of ΦCpeT at 1.8-Å resolution revealed that the protein adopts a similar fold as the cyanobacterial T-type lyase CpcT from sp. PCC7120 but overall is more compact and smaller. ΦCpeT specifically binds phycoerythrobilin (PEB) leading to a tight complex that can also be formed in when it is co-expressed with genes encoding PEB biosynthesis ( and ). The formed ΦCpeT·PEB complex was very stable as the chromophore was not lost during chromatography and displayed a strong red fluorescence with a fluorescence quantum yield of Φ = 0.3. This complex was not directly able to transfer PEB to the host phycobiliprotein ß-subunit. However, it could assist the host lyase CpeS in its function by providing a pool of readily available PEB, a feature that might be important for fast phycobiliprotein assembly during phage infection.
[Mh] Termos MeSH primário: Bacteriófagos/química
Liases/química
Ficobiliproteínas/química
Proteínas Virais/química
[Mh] Termos MeSH secundário: Bacteriófagos/metabolismo
Cristalografia por Raios X
Liases/metabolismo
Modelos Moleculares
Nostoc/química
Nostoc/enzimologia
Nostoc/metabolismo
Ficobilinas/metabolismo
Ficobiliproteínas/metabolismo
Ficoeritrina/metabolismo
Prochlorococcus/virologia
Conformação Proteica
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phycobilins); 0 (Phycobiliproteins); 0 (Viral Proteins); 11016-17-4 (Phycoerythrin); 18097-67-1 (phycoerythrobilin); EC 4.- (Lyases)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170619
[Lr] Data última revisão:
170619
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170112
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M116.769703


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[PMID]:27668842
[Au] Autor:Xia X; Partensky F; Garczarek L; Suzuki K; Guo C; Yan Cheung S; Liu H
[Ad] Endereço:Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong.
[Ti] Título:Phylogeography and pigment type diversity of Synechococcus cyanobacteria in surface waters of the northwestern pacific ocean.
[So] Source:Environ Microbiol;19(1):142-158, 2017 Jan.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The widespread unicellular cyanobacteria Synechococcus are major contributors to global marine primary production. Here, we report their abundance, phylogenetic diversity (as assessed using the RNA polymerase gamma subunit gene rpoC1) and pigment diversity (as indirectly assessed using the laterally transferred cpeBA genes, encoding phycoerythrin-I) in surface waters of the northwestern Pacific Ocean, sampled over nine distinct cruises (2008-2015). Abundance of Synechococcus was low in the subarctic ocean and South China Sea, intermediate in the western subtropical Pacific Ocean, and the highest in the Japan and East China seas. Clades I and II were by far the most abundant Synechococcus lineages, the former dominating in temperate cold waters and the latter in (sub)tropical waters. Clades III and VI were also fairly abundant in warm waters, but with a narrower distribution than clade II. One type of chromatic acclimater (3dA) largely dominated the Synechococcus communities in the subarctic ocean, while another (3dB) and/or cells with a fixed high phycourobilin to phycoerythrobilin ratio (pigment type 3c) predominated at mid and low latitudes. Altogether, our results suggest that the variety of pigment content found in most Synechococcus clades considerably extends the niches that they can colonize and therefore the whole genus habitat.
[Mh] Termos MeSH primário: Pigmentos Biológicos/metabolismo
Água do Mar/microbiologia
Synechococcus/classificação
Synechococcus/genética
Synechococcus/metabolismo
[Mh] Termos MeSH secundário: China
Ecossistema
Japão
Oceano Pacífico
Ficobilinas/análise
Ficobilinas/metabolismo
Ficoeritrina/análise
Ficoeritrina/metabolismo
Filogenia
Filogeografia
Pigmentos Biológicos/análise
Synechococcus/isolamento & purificação
Urobilina/análogos & derivados
Urobilina/análise
Urobilina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phycobilins); 0 (Pigments, Biological); 11016-17-4 (Phycoerythrin); 18097-67-1 (phycoerythrobilin); 1856-98-0 (Urobilin); 61932-71-6 (phycourobilin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160927
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.13541


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[PMID]:27633748
[Au] Autor:Mysliwa-Kurdziel B; Solymosi K
[Ad] Endereço:Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
[Ti] Título:Phycobilins and Phycobiliproteins Used in Food Industry and Medicine.
[So] Source:Mini Rev Med Chem;17(13):1173-1193, 2017.
[Is] ISSN:1875-5607
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Open tetrapyrroles termed phycobilins represent the major photosynthetic accessory pigments of several cyanobacteria and some eukaryotic algae such as the Glaucophyta, Cryptophyta and Rhodophyta. These pigments are covalently bound to so-called phycobiliproteins which are in general organized into phycobilisomes on the thylakoid membranes. OBJECTIVE & METHODS: In this work we first briefly describe the physico-chemical properties, biosynthesis, occurrence, in vivo localization and roles of the phycobilin pigments and the phycobiliproteins. Then the potential applications and uses of these pigments, pigment-protein complexes and related products by the food industry (e.g., as LinaBlue® or the so-called spirulina extract used as coloring food), by the health industry or as fluorescent dyes are critically reviewed. CONCLUSION: In addition to the stability, bioavailability and safety issues of purified phycobilins and phycobiliproteins, literature data about their antioxidant, anticancer, anti-inflammatory, immunomodulatory, hepatoprotective, nephroprotective and neuroprotective effects, and their potential use in photodynamic therapy (PDT) are also discussed.
[Mh] Termos MeSH primário: Corantes de Alimentos/química
Ficobilinas/biossíntese
Ficobiliproteínas/biossíntese
[Mh] Termos MeSH secundário: Anti-Inflamatórios/química
Anti-Inflamatórios/metabolismo
Anti-Inflamatórios/uso terapêutico
Antineoplásicos/química
Antineoplásicos/metabolismo
Antineoplásicos/uso terapêutico
Antioxidantes/química
Antioxidantes/metabolismo
Antioxidantes/uso terapêutico
Doenças Cardiovasculares/patologia
Doenças Cardiovasculares/prevenção & controle
Criptófitas/química
Criptófitas/metabolismo
Seres Humanos
Fatores Imunológicos/química
Fatores Imunológicos/metabolismo
Fatores Imunológicos/uso terapêutico
Neoplasias/patologia
Neoplasias/prevenção & controle
Doenças Neurodegenerativas/patologia
Doenças Neurodegenerativas/prevenção & controle
Ficobilinas/química
Ficobiliproteínas/química
Rodófitas/química
Rodófitas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antineoplastic Agents); 0 (Antioxidants); 0 (Food Coloring Agents); 0 (Immunologic Factors); 0 (Phycobilins); 0 (Phycobiliproteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160917
[St] Status:MEDLINE
[do] DOI:10.2174/1389557516666160912180155


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[PMID]:27959940
[Au] Autor:Radibratovic M; Minic S; Stanic-Vucinic D; Nikolic M; Milcic M; Cirkovic Velickovic T
[Ad] Endereço:Institute of Chemistry, Technology and Metallurgy - Center for Chemistry, University of Belgrade, Belgrade, Serbia.
[Ti] Título:Stabilization of Human Serum Albumin by the Binding of Phycocyanobilin, a Bioactive Chromophore of Blue-Green Alga Spirulina: Molecular Dynamics and Experimental Study.
[So] Source:PLoS One;11(12):e0167973, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Phycocyanobilin (PCB) binds with high affinity (2.2 x 106 M-1 at 25°C) to human serum albumin (HSA) at sites located in IB and IIA subdomains. The aim of this study was to examine effects of PCB binding on protein conformation and stability. Using 300 ns molecular dynamics (MD) simulations, UV-VIS spectrophotometry, CD, FT-IR, spectrofluorimetry, thermal denaturation and susceptibility to trypsin digestion, we studied the effects of PCB binding on the stability and rigidity of HSA, as well as the conformational changes in PCB itself upon binding to the protein. MD simulation results demonstrated that HSA with PCB bound at any of the two sites showed greater rigidity and lower overall and individual domain flexibility compared to free HSA. Experimental data demonstrated an increase in the α-helical content of the protein and thermal and proteolytic stability upon ligand binding. PCB bound to HSA undergoes a conformational change to a more elongated conformation in the binding pockets of HSA. PCB binding to HSA stabilizes the structure of this flexible transport protein, making it more thermostable and resistant to proteolysis. The results from this work explain at molecular level, conformational changes and stabilization of HSA structure upon ligand binding. The resultant increased thermal and proteolytic stability of HSA may provide greater longevity to HSA in plasma.
[Mh] Termos MeSH primário: Simulação de Acoplamento Molecular
Ficobilinas/farmacologia
Ficocianina/farmacologia
Albumina Sérica/química
[Mh] Termos MeSH secundário: Sítios de Ligação
Ficobilinas/química
Ficocianina/química
Ligação Proteica
Estabilidade Proteica
Albumina Sérica/metabolismo
Spirulina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phycobilins); 0 (Serum Albumin); 11016-15-2 (Phycocyanin); 36NUT04V2K (phycocyanobilin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170629
[Lr] Data última revisão:
170629
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161214
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0167973


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[PMID]:27497182
[Au] Autor:Basdeo SA; Campbell NK; Sullivan LM; Flood B; Creagh EM; Mantle TJ; Fletcher JM; Dunne A
[Ad] Endereço:School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, The University of Dublin, Dublin 2, Ireland.
[Ti] Título:Suppression of human alloreactive T cells by linear tetrapyrroles; relevance for transplantation.
[So] Source:Transl Res;178:81-94.e2, 2016 Dec.
[Is] ISSN:1878-1810
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The main limitation to successful transplantation is the antigraft response developed by the recipient immune system, and the adverse side effects of immunosuppressive agents which are associated with significant toxicity and counter indications such as infection and cancer. Furthermore, immunosuppressants do little to prevent ischemia-reperfusion injury during the transplantation procedure itself hence there is a growing need to develop novel immunosuppressive drugs specifically aimed at prolonging graft survival. Linear tetrapyrroles derived from the breakdown of mammalian heme have been shown in numerous studies to play a protective role in allograft transplantation and ischemia-reperfusion injury; however, commercial sources of these products have not been approved for use in humans. Plants and algae produce equivalent linear tetrapyrroles called bilins that serve as chromophores in light-sensing. One such marine-derived tetrapyrrole, phycocyanobilin (PCB), shows significant structural similarity to mammalian biliverdin (BV) and may prove to be a safer alternative for use in the clinic if it can exert direct effects on human immune cells. Using a mixed lymphocyte reaction, we quantified the allogeneic responses of recipient cells to donor cells and found that PCB, like BV, effectively suppressed proliferation and proinflammatory cytokine production. In addition, we found that BV and PCB can directly downregulate the proinflammatory responses of both innate dendritic cells and adaptive T cells. We therefore propose that PCB may be an effective therapeutic drug in the clinical setting of transplantation and may also have wider applications in regulating inappropriate inflammation.
[Mh] Termos MeSH primário: Linfócitos T CD4-Positivos/efeitos dos fármacos
Linfócitos T CD4-Positivos/imunologia
Tetrapirróis/farmacologia
[Mh] Termos MeSH secundário: Animais
Antioxidantes/farmacologia
Antioxidantes/uso terapêutico
Biliverdina/farmacologia
Biliverdina/uso terapêutico
Complexo CD3/metabolismo
Proliferação Celular/efeitos dos fármacos
Citocinas/biossíntese
Células Dendríticas/efeitos dos fármacos
Células Dendríticas/imunologia
Feminino
Seres Humanos
Imunossupressores/farmacologia
Imunossupressores/uso terapêutico
Inflamação/patologia
Ativação Linfocitária/efeitos dos fármacos
Camundongos Endogâmicos C57BL
Ficobilinas/farmacologia
Ficobilinas/uso terapêutico
Ficocianina/farmacologia
Ficocianina/uso terapêutico
Traumatismo por Reperfusão/tratamento farmacológico
Traumatismo por Reperfusão/prevenção & controle
Linfócitos T Reguladores/efeitos dos fármacos
Linfócitos T Reguladores/imunologia
Células Th1/efeitos dos fármacos
Células Th1/imunologia
Transplante Homólogo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (CD3 Complex); 0 (Cytokines); 0 (Immunosuppressive Agents); 0 (Phycobilins); 0 (Tetrapyrroles); 11016-15-2 (Phycocyanin); 36NUT04V2K (phycocyanobilin); O9MIA842K9 (Biliverdine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:160807
[St] Status:MEDLINE


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[PMID]:27495242
[Au] Autor:Bemal S; Anil AC
[Ad] Endereço:School of Oceanography, Academy of Scientific and Innovative Research, Council of Scientific and Industrial Research, National Institute of Oceanography (CSIR-NIO), Dona-Paula 403004, Goa, India.
[Ti] Título:Genetic and ecophysiological traits of Synechococcus strains isolated from coastal and open ocean waters of the Arabian Sea.
[So] Source:FEMS Microbiol Ecol;92(11), 2016 Nov.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The picocyanobacterium Synechococcus is a prominent primary producer in the marine environment. The marine Synechococcus strains are clustered into different clades representing ecologically distinct genotypes. In this study, we compared phylogeny, photophysiology and cell cycles of four novel phycoerythrin-containing Synechococcus strains (clade II of subcluster 5.1) isolated from different depths of the water column (surface and subsurface waters) in coastal and offshore regions of the eastern Arabian Sea. The surface water strains possessed a lesser number of thylakoid layers and had a higher zeaxanthin to chlorophyll a ratio than subsurface strains indicating possible influence of light intensity available at their niche. The DNA distribution pattern of the four strains was bimodal in optimal cellular physiology conditions with cell division restricted to the light period and synchronized with the light-dark cycle. The presence of phycourobilin or phycoerythrobilin and the ratio between these two chromophores in all four strains varied according to available spectral wavelength in situ This study indicates that the timing of cell division is conserved within these genotypically identical Synechococcus strains, despite their having different chromophore ratios. We conclude that the timing of cell division of the Synechococcus strains has a genetic basis rather than being determined by phenotypic characters, such as chromophore content and ratio.
[Mh] Termos MeSH primário: Divisão Celular/genética
Fotossíntese/fisiologia
Synechococcus/crescimento & desenvolvimento
Synechococcus/fisiologia
[Mh] Termos MeSH secundário: Ásia Ocidental
Divisão Celular/fisiologia
Clorofila/análogos & derivados
Clorofila/metabolismo
DNA Bacteriano/genética
Luz
Microscopia Eletrônica de Transmissão
Oceanos e Mares
Fenótipo
Ficobilinas/metabolismo
Ficoeritrina/metabolismo
Filogenia
Synechococcus/genética
Synechococcus/isolamento & purificação
Urobilina/análogos & derivados
Urobilina/metabolismo
Água/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Phycobilins); 059QF0KO0R (Water); 11016-17-4 (Phycoerythrin); 1406-65-1 (Chlorophyll); 18097-67-1 (phycoerythrobilin); 1856-98-0 (Urobilin); 22309-13-3 (chlorophyll a'); 61932-71-6 (phycourobilin); YF5Q9EJC8Y (chlorophyll a)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160807
[St] Status:MEDLINE


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[PMID]:27246537
[Au] Autor:Sugie Y; Hori M; Oka S; Ohtsuka H; Aiba H
[Ad] Endereço:Laboratory of Molecular Microbiology, Graduate School of Bioagricultural Sciences, Nagoya University.
[Ti] Título:Reconstruction of a chromatic response system in Escherichia coli.
[So] Source:J Gen Appl Microbiol;62(3):140-3, 2016 Jul 14.
[Is] ISSN:1349-8037
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Two-component signal transduction systems (TCS) are involved in widespread cellular responses to diverse signals from bacteria to plants. Cyanobacteria have evolved photoperception systems for efficient photosynthesis, and some histidine kinases are known to function as photosensors. In this study, we attempt to reconstruct the photoperception system in Escherichia coli to make an easily controllable ON/OFF switch for gene expressions. For this purpose, a CcaS-CcaR two-component system from Nostoc punctiforme was expressed with phycocyanobilin (PCB) producing enzymes in E. coli which carries a G-box-controlled reporter gene. We succeeded to endow E. coli with a gene activation switch that is regulated in a light-color dependent manner. The possibility of such a switch for the development of synthetic biology is pointed out.
[Mh] Termos MeSH primário: Escherichia coli/genética
Escherichia coli/metabolismo
Engenharia Genética/métodos
Transdução de Sinal Luminoso
Luz
Fotorreceptores Microbianos/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Escherichia coli/enzimologia
Regulação Bacteriana da Expressão Gênica
Nostoc/genética
Fotorreceptores Microbianos/genética
Fotossíntese/genética
Ficobilinas/metabolismo
Ficocianina/metabolismo
Biologia Sintética/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Photoreceptors, Microbial); 0 (Phycobilins); 11016-15-2 (Phycocyanin); 36NUT04V2K (phycocyanobilin)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170407
[Lr] Data última revisão:
170407
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160602
[St] Status:MEDLINE
[do] DOI:10.2323/jgam.2016.01.006



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