Base de dados : MEDLINE
Pesquisa : D03.383.246.238 [Categoria DeCS]
Referências encontradas : 1331 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 134 ir para página                         

  1 / 1331 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29355526
[Au] Autor:Bosc E; Nastri J; Lefort V; Valli M; Contiguiba F; Pioli R; Furlan M; Bolzani VDS; El Amri C; Reboud-Ravaux M
[Ad] Endereço:Sorbonne Université, UPMC Univ Paris 06-CNRS, IBPS, UMR 8256, Inserm ERL1164, B2A, 7 Quai Saint Bernard, F75005 Paris, France.
[Ti] Título:Piperlongumine and some of its analogs inhibit selectively the human immunoproteasome over the constitutive proteasome.
[So] Source:Biochem Biophys Res Commun;496(3):961-966, 2018 02 12.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The natural small molecule piperlongumine A is toxic selectively to cancer cells in vitro and in vivo. This toxicity has been correlated with cancer cell ROS, DNA damage and apoptotic cell death increases. We demonstrate here a new mechanistic property of piperlongumine: it inhibits selectively human immunoproteasome with no noticeable inhibition of human constitutive proteasome. This result suggests that immunoproteasome inhibition, a mechanism independent of ROS elevation, may also partly play a role in the anticancer effects observed with piperlongumine. Structure-activity relationships of piperlongumine analogs suggest that the lactam (piperidonic) ring of piperlongumine A may be replaced by the linear olefin -NHCO-CH =CH to improve both in vitro inhibitory efficiency against immunoproteasome and cellular toxicity.
[Mh] Termos MeSH primário: Apoptose/imunologia
Dioxolanos/química
Dioxolanos/imunologia
Imunoproteínas/química
Imunoproteínas/imunologia
Complexo de Endopeptidases do Proteassoma/química
Complexo de Endopeptidases do Proteassoma/imunologia
[Mh] Termos MeSH secundário: Apoptose/efeitos dos fármacos
Dioxolanos/administração & dosagem
Relação Dose-Resposta a Droga
Avaliação Pré-Clínica de Medicamentos
Células HeLa
Seres Humanos
Ligação Proteica
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Dioxolanes); 0 (Immunoproteins); EC 3.4.25.1 (Proteasome Endopeptidase Complex); HN39MC8KIO (piperlonguminine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180123
[St] Status:MEDLINE


  2 / 1331 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29406096
[Au] Autor:Dong X; Zhang L; Chen M; Yang Z; Zuo Z; Wang C
[Ad] Endereço:State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, China. Electronic address: 402127413@qq.com.
[Ti] Título:Exposure to difenoconazole inhibits reproductive ability in male marine medaka (Oryzias melastigma).
[So] Source:J Environ Sci (China);63:126-132, 2018 Jan.
[Is] ISSN:1001-0742
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Difenoconazole (DFZ) is a triazole fungicide which has been detected in the aquatic environment, including estuaries and embayments. However, few studies addressing the reproductive toxicity and transgenerational effects of DFZ on marine fishes are available. The present study was conducted to investigate the effects of DFZ on male marine medaka (Oryzias melastigma). After exposure of the embryo to 1, 10, 100 and 1000ng/L DFZ for 180days, the gonadosomatic index was significantly decreased in the 1000ng/L treatment. The number of sperm was reduced while the abundances of spermatocytes and spermatogonia in the testes were increased in all the treatments. The mRNA levels of salmon-type gnrh (sgnrh), the luteinizing hormone (lhß) and the follicle-stimulating hormone (fshß) genes in the brain all exhibited a significant down-regulation, the expression of androgen receptors (arα and arß) was decreased and that of estrogen receptor ß and cytochrome P450 aromatase (cyp19B) was increased in the testes. The expression levels of cyp19A and cyp19B were increased in the liver. The decrease of ars mRNA levels might be one of the reasons causing the reduction of sperm. The down-regulation of sgnrh, lhß and fshß mRNA levels suggested that DFZ might impact the spermatogenesis via the brain-pituitary-gonad pathway. The decrease of the fertilization success, the hatch ability and the swim-up success in the F1 generation indicated that DFZ pollution at environmental levels might cause a decrease of wild fish populations.
[Mh] Termos MeSH primário: Dioxolanos/toxicidade
Fungicidas Industriais/toxicidade
Oryzias/fisiologia
Triazóis/toxicidade
[Mh] Termos MeSH secundário: Animais
Aromatase/metabolismo
Masculino
Receptores Androgênicos
Reprodução/efeitos dos fármacos
Testículo
Poluentes Químicos da Água/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dioxolanes); 0 (Fungicides, Industrial); 0 (Receptors, Androgen); 0 (Triazoles); 0 (Water Pollutants, Chemical); D9612XCH4P (difenoconazole); EC 1.14.14.1 (Aromatase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180207
[St] Status:MEDLINE


  3 / 1331 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:29061824
[Au] Autor:Chou PY; Lee MM; Lin SY; Sheu MJ
[Ad] Endereço:Department of Nursing, Hung Kuang University, Taichung, Taiwan, R.O.C.
[Ti] Título:Pipoxolan Exhibits Antitumor Activity Toward Oral Squamous Cell Carcinoma Through Reactive Oxygen Species-mediated Apoptosis.
[So] Source:Anticancer Res;37(11):6391-6400, 2017 11.
[Is] ISSN:1791-7530
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Pipoxolan is frequently prescribed as a smooth muscle relaxant. Pipoxolan has also been shown to have anticancer activity. Our study investigated whether pipoxolan induced apoptosis in oral squamous cell carcinoma (OSCC). Cell cytotoxicity was evaluated by the MTT assay. Cell apoptosis and cell-cycle distribution were measured by annexin V/propidium iodide (PI) double staining and flow cytometry, respectively. Apoptotic-related proteins were assessed by western blotting. Reactive oxygen species (ROS) generation and mitochondrial membrane potential (MMP) were measured with fluorescent probes. Following exposure of TW206 OSCC cells to pipoxolan, a time-dependently decrease in MMP and an increase in ROS were observed. However, these effects were significantly abrogated by the free radical scavenger N-acetyl-L-cysteine. Since high levels of ROS were produced early in the treatment, intracellular ROS seemed to play a key role in pipoxolan-induced apoptosis. In HSC-3 OSCC cells, our results demonstrated that pipoxolan treatment caused a time-dependent increase of protein expression of active caspase-3 and -9, cytosolic cytochrome c, cleavage of poly (ADP-ribose) polymerase, and B-cell lymphoma 2 (BCL2)-like protein 4 (BAX). However, expression of BCL2 itself was reduced. Clearly, such an increase in BAX/BCL2 ratio would be associated with apoptosis. In addition, pipoxolan markedly suppressed the protein expression of phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K) and phosphorylation of protein kinase B (AKT). These data suggest that pipoxolan acts against HSC-3 in vitro via intrinsic apoptotic signaling pathways, and inhibition of PI3K/AKT signaling.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Carcinoma de Células Escamosas/metabolismo
Dioxolanos/farmacologia
Neoplasias Bucais/metabolismo
Espécies Reativas de Oxigênio/metabolismo
[Mh] Termos MeSH secundário: Apoptose
Proteínas Reguladoras de Apoptose/metabolismo
Carcinoma de Células Escamosas/tratamento farmacológico
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Ensaios de Seleção de Medicamentos Antitumorais
Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos
Seres Humanos
Metaloproteinases da Matriz/metabolismo
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Neoplasias Bucais/tratamento farmacológico
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Apoptosis Regulatory Proteins); 0 (Dioxolanes); 0 (Reactive Oxygen Species); 493GZJ5T6I (pipoxolan); EC 3.4.24.- (Matrix Metalloproteinases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171025
[St] Status:MEDLINE


  4 / 1331 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28802122
[Au] Autor:Wiemann J; Karasch J; Loesche A; Heller L; Brandt W; Csuk R
[Ad] Endereço:Martin-Luther University Halle-Wittenberg, Organic Chemistry, Kurt-Mothes Str. 2, D-06120 Halle (Saale), Germany.
[Ti] Título:Piperlongumine B and analogs are promising and selective inhibitors for acetylcholinesterase.
[So] Source:Eur J Med Chem;139:222-231, 2017 Oct 20.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Piperlongumine B (19), an alkaloid previously isolated from long pepper (Piper longum) has been synthesized for the first time in a short sequence and in good yield together with 19 analogs. Screening of these compounds in Ellman's assays showed several of them to be good inhibitors of acetylcholinesterase while being less active for butyrylcholinesterase. Activity of the compounds increased with the ring size of the heterocycle, and a maximum of activity was observed for an analog holding 12 methylene groups in the aliphatic side chain. These compounds may be regarded as promising candidates for the development of efficient inhibitors of acetylcholinesterase being useful for the treatment of Alzheimer's disease.
[Mh] Termos MeSH primário: Acetilcolinesterase/metabolismo
Inibidores da Colinesterase/farmacologia
Dioxolanos/farmacologia
[Mh] Termos MeSH secundário: Animais
Butirilcolinesterase/metabolismo
Inibidores da Colinesterase/síntese química
Inibidores da Colinesterase/química
Dioxolanos/síntese química
Dioxolanos/química
Relação Dose-Resposta a Droga
Electrophorus/metabolismo
Estrutura Molecular
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cholinesterase Inhibitors); 0 (Dioxolanes); EC 3.1.1.7 (Acetylcholinesterase); EC 3.1.1.8 (Butyrylcholinesterase); HN39MC8KIO (piperlonguminine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170813
[St] Status:MEDLINE


  5 / 1331 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28778471
[Au] Autor:Zhang Q; Cao R; Liu A; Lei S; Li Y; Yang J; Li S; Xiao J
[Ad] Endereço:School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, Liaoning, China; Laboratory of Computer-Aided Drug Design and Discovery, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China. Electronic address: qiyanzhang1113@163.com.
[Ti] Título:Design, synthesis and evaluation of 2,2-dimethyl-1,3-dioxolane derivatives as human rhinovirus 3C protease inhibitors.
[So] Source:Bioorg Med Chem Lett;27(17):4061-4065, 2017 09 01.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The human rhinovirus (HRV) is the most significant cause of the common cold all over the world. The maturation and replication of this virus entirely depend on the activity of a virus-encoded 3C protease. Due to the high conservation among different serotypes and the minimal homology existing between 3C protease and known mammalian enzymes, 3C protease has been regarded as an attractive target for the treatment of HRV infections. In this study, we identified a novel (4R,5R)-N -(2-((3-methoxyphenyl)amino)ethyl)-2,2-dimethyl-N -(naphthalen-2-yl)-1,3-dioxolane-4,5-dicarboxamide (7a) to be a HRV 3C protease inhibitor via virtual screening. Further research has been focused on the design, synthesis and in vitro biological evaluation of 7a derivatives. The studies revealed that compound 7d has an IC value of 2.50±0.7µM against HRV 3C protease, and it thus could serve as a promising compound for the development of novel anti-rhinoviral medicines.
[Mh] Termos MeSH primário: Dioxolanos/farmacologia
Desenho de Drogas
Inibidores de Proteases/farmacologia
Rhinovirus/enzimologia
Proteínas Virais/antagonistas & inibidores
[Mh] Termos MeSH secundário: Cisteína Endopeptidases/metabolismo
Dioxolanos/síntese química
Dioxolanos/química
Relação Dose-Resposta a Droga
Seres Humanos
Estrutura Molecular
Inibidores de Proteases/síntese química
Inibidores de Proteases/química
Relação Estrutura-Atividade
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Dioxolanes); 0 (Protease Inhibitors); 0 (Viral Proteins); EC 3.4.22.- (Cysteine Endopeptidases); EC 3.4.22.28 (3C proteases); Y57RBG19JL (formal glycol)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170806
[St] Status:MEDLINE


  6 / 1331 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28686911
[Au] Autor:Zou Y; Yan C; Zhang H; Xu J; Zhang D; Huang Z; Zhang Y
[Ad] Endereço:State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China; Jiangsu Key Laboratory of Drug Discovery for Metabolic Diseases, China Pharmaceutical University, Nanjing 210009, PR China.
[Ti] Título:Synthesis and evaluation of N-heteroaromatic ring-based analogs of piperlongumine as potent anticancer agents.
[So] Source:Eur J Med Chem;138:313-319, 2017 Sep 29.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Piperlongumine (PL) selectively targets a wide spectrum of cancer cells and induces their death by triggering various pathways, including apoptosis, necrosis and autophagy. However, the poor solubility is a serious concern for intensive study and clinical application. We synthesized its analogs 1-9 by replacement of the trimethoxyphenyl of PL with an N-heteroaromatic ring and/or not introduction of 2-Cl. These compounds improved aqueous solubility and displayed potent anticancer activity. The most active compound 9 selectively enhanced ROS levels in colon cancer cells and inhibited the cell proliferation but sparing non-tumor colon cells. Importantly, 9 significantly repressed tumor growth in an HCT-116 xenograft mouse model, suggesting that these N-heteroaromatic ring-based analogs of PL warrant further investigation.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Dioxolanos/farmacologia
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/síntese química
Antineoplásicos/química
Linhagem Celular
Proliferação Celular/efeitos dos fármacos
Dioxolanos/síntese química
Dioxolanos/química
Relação Dose-Resposta a Droga
Ensaios de Seleção de Medicamentos Antitumorais
Células HCT116
Seres Humanos
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Nus
Estrutura Molecular
Neoplasias Experimentais/tratamento farmacológico
Neoplasias Experimentais/patologia
Solubilidade
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Dioxolanes); HN39MC8KIO (piperlonguminine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170708
[St] Status:MEDLINE


  7 / 1331 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Texto completo
[PMID]:28521067
[Au] Autor:Brigo F; Igwe SC; Bragazzi NL
[Ad] Endereço:Department of Neuroscience, Biomedicine and Movement, University of Verona, P.le L.A. Scuro, 10, Verona, Verona, Italy, 37134.
[Ti] Título:Antiepileptic drugs for the treatment of infants with severe myoclonic epilepsy.
[So] Source:Cochrane Database Syst Rev;5:CD010483, 2017 05 18.
[Is] ISSN:1469-493X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: This is an updated version of the original Cochrane review published in 2015, Issue 10.Severe myoclonic epilepsy in infants (SMEI), also known as Dravet syndrome, is a rare, refractory form of epilepsy, for which stiripentol (STP) has been recently licensed as add-on therapy. OBJECTIVES: To evaluate the efficacy and tolerability of STP and other antiepileptic drug treatments (including ketogenic diet) for patients with SMEI. SEARCH METHODS: For the latest update we searched the Cochrane Epilepsy Group Specialized Register (20 December 2016), the Cochrane Central Register of Controlled Trials (CENTRAL) via the Cochrane Register of Studies Online (CRSO, 20 December 2016), MEDLINE (Ovid, 1946 to 20 December 2016) and ClinicalTrials.gov (20 December 2016). Previously we searched the World Health Organization (WHO) International Clinical Trials Registry Platform ICTRP, but this was not usable at the time of this update. We also searched the bibliographies of identified studies for additional references. We handsearched selected journals and conference proceedings and imposed no language restrictions. SELECTION CRITERIA: Randomised controlled trials (RCTs) or quasi-randomised controlled trials; double- or single-blinded or unblinded trials; and parallel-group studies. Administration of at least one antiepileptic drug therapy given singly (monotherapy) or in combination (add-on therapy) compared with add-on placebo or no add-on treatment. DATA COLLECTION AND ANALYSIS: Review authors independently selected trials for inclusion according to predefined criteria, extracted relevant data and evaluated the methodological quality of trials. We assessed the following outcomes: 50% or greater seizure reduction, seizure freedom, adverse effects, proportion of dropouts and quality of life. We assessed outcomes by using a Mantel-Haenszel meta-analysis to calculate risk ratios (RRs) with 95% confidence intervals (95% CIs). MAIN RESULTS: Since the last version of this review no new studies have been found. Specifically, we found no RCTs assessing drugs other than STP. The review includes two RCTs evaluating use of STP (total of 64 children). Both studies were generally at unclear risk of bias. A significantly higher proportion of participants had 50% or greater reduction in seizure frequency in the STP group compared with the placebo group (22/33 versus 2/31; RR 10.40, 95% CI 2.64 to 40.87). A significantly higher proportion of participants achieved seizure freedom in the STP group compared with the placebo group (12/33 versus 1/31; RR 7.93, 95% CI 1.52 to 41.21). Investigators found no significant differences in proportions of dropouts from the STP group compared with the placebo group (2/33 versus 8/31; RR 0.24, 95% CI 0.06 to 1.03). Only one study explicitly reported the occurrence of side effects, noting that higher proportions of participants in the STP group experienced side effects than in the placebo group (100% versus 25%; RR 3.73, 95% CI 1.81 to 7.67). We rated the quality of the evidence as low to moderate according to GRADE criteria, as most information is from studies judged to be at an unclear risk of bias. AUTHORS' CONCLUSIONS: Data derived from two small RCTs indicate that STP is significantly better than placebo with regards to 50% or greater reduction in seizure frequency and seizure freedom. Adverse effects occurred more frequently with STP. Additional adequately powered studies with long-term follow-up should be conducted to unequivocally establish the long-term efficacy and tolerability of STP in the treatment of patients with SMEI.
[Mh] Termos MeSH primário: Anticonvulsivantes/uso terapêutico
Dioxolanos/uso terapêutico
Epilepsias Mioclônicas/tratamento farmacológico
[Mh] Termos MeSH secundário: Adolescente
Anticonvulsivantes/efeitos adversos
Criança
Pré-Escolar
Dioxolanos/efeitos adversos
Feminino
Seres Humanos
Masculino
Pacientes Desistentes do Tratamento/estatística & dados numéricos
Ensaios Clínicos Controlados Aleatórios como Assunto
Convulsões/tratamento farmacológico
[Pt] Tipo de publicação:JOURNAL ARTICLE; META-ANALYSIS; REVIEW
[Nm] Nome de substância:
0 (Anticonvulsants); 0 (Dioxolanes); R02XOT8V8I (stiripentol)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171117
[Lr] Data última revisão:
171117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170519
[St] Status:MEDLINE
[do] DOI:10.1002/14651858.CD010483.pub4


  8 / 1331 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28434764
[Au] Autor:Zhang Y; Ma H; Wu Y; Wu Z; Yao Z; Zhang W; Zhuang C; Miao Z
[Ad] Endereço:Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.
[Ti] Título:Novel non-trimethoxylphenyl piperlongumine derivatives selectively kill cancer cells.
[So] Source:Bioorg Med Chem Lett;27(11):2308-2312, 2017 06 01.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Piperlongumine (PL) is a natural alkaloid with broad biological activities. Twelve analogues have been designed and synthesized with non-substituted benzyl rings or heterocycles in this work. Most of the compounds showed better anticancer activities than the parent PL without apparent toxicity in normal cells. Elevation of cellular ROS levels was one of the main anticancer mechanisms of these compounds. Cell apoptosis and cell cycle arrest for the best compound ZM90 were evaluated and similar mechanism of action with PL was demonstrated. The SAR was also characterized, providing worthy directions for further optimization of PL compounds.
[Mh] Termos MeSH primário: Dioxolanos/farmacologia
[Mh] Termos MeSH secundário: Apoptose/efeitos dos fármacos
Linhagem Celular Tumoral
Dioxolanos/química
Ensaios de Seleção de Medicamentos Antitumorais
Seres Humanos
Espécies Reativas de Oxigênio/metabolismo
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Dioxolanes); 0 (Reactive Oxygen Species); HN39MC8KIO (piperlonguminine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171124
[Lr] Data última revisão:
171124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170425
[St] Status:MEDLINE


  9 / 1331 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28432496
[Au] Autor:Dong X; Zuo Z; Guo J; Li H; Zhang L; Chen M; Yang Z; Wang C
[Ad] Endereço:State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, PR China.
[Ti] Título:Reproductive effects of life-cycle exposure to difenoconazole on female marine medaka (Oryzias melastigma).
[So] Source:Ecotoxicology;26(6):772-781, 2017 Aug.
[Is] ISSN:1573-3017
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Difenoconazole (DFZ) is a widely used triazole fungicide which has been detected in some estuaries and embayments. This study was conducted to investigate the effects of DFZ on ovarian development in female marine medaka (Oryzias melastigma). After 180 days exposure of the embryo to DFZ (0, 1, 10, 100 and 1000 ng/L), the gonadosomatic index and percentage of mature oocytes produced were significantly reduced in the 1, 10 and 100 ng/L treatments but not the 1000 ng/L treatment compared to the control, thus exhibiting a U-shaped dose response curve. The relative mRNA levels of brain follicle-stimulating hormone, ovarian cytochrome P450 aromatase (CYP19s), hepatic estrogen receptors and vitellogenin, and the ratio of 17ß-estradiol to testosterone in the muscle, also showed a U-shaped dose response, which was consistent with the development of oocytes. In addition, glutathione S-transferase activity in the ovary showed a U-shaped dose-response. These results gave an explanation for this U-shaped dose-response. The egg number produced, the hatch ability and the swim-up success in the F1 generation all showed a U-shaped dose response, indicating that exposure to DFZ at low concentrations can cause a decrease of fecundity and viability of the next generation. Thus, a more extensive evaluation of the impact of DFZ on marine fish reproduction at realistic environmental concentrations is needed.
[Mh] Termos MeSH primário: Dioxolanos/toxicidade
Fungicidas Industriais/toxicidade
Oryzias/fisiologia
Triazóis/toxicidade
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Estradiol/metabolismo
Feminino
Glutationa Transferase/metabolismo
Estágios do Ciclo de Vida
Oryzias/crescimento & desenvolvimento
Ovário/efeitos dos fármacos
Vitelogeninas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dioxolanes); 0 (Fungicides, Industrial); 0 (Triazoles); 0 (Vitellogenins); 0 (Water Pollutants, Chemical); 4TI98Z838E (Estradiol); D9612XCH4P (difenoconazole); EC 2.5.1.18 (Glutathione Transferase)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170423
[St] Status:MEDLINE
[do] DOI:10.1007/s10646-017-1808-1


  10 / 1331 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28402027
[Au] Autor:Kang D; Zhang H; Chen Y; Wang F; Shi L; Hu D; Zhang K
[Ad] Endereço:State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, 550025, China.
[Ti] Título:Simultaneous determination of difenoconazole, trifloxystrobin and its metabolite trifloxystrobin acid residues in watermelon under field conditions by GC-MS/MS.
[So] Source:Biomed Chromatogr;31(11), 2017 Nov.
[Is] ISSN:1099-0801
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An optimized quick, easy, cheap, effective, rugged and safe method for the simultaneous determination of difenoconazole, trifloxystrobin and its metabolite trifloxystrobin acid residues in watermelon and soil was developed and validated by gas chromatography with tandem mass spectrometry. The samples were extracted with acetonitrile (1% formic acid) and cleaned up by dispersive solid-phase extraction with octadecylsilane sorbent. The limit of quantification of the method was 0.01 mg/kg, and the limit of detection was 0.003 mg/kg for all three analytes. The recoveries of the fungicides in watermelon, pulp and soil were 72.32-99.20% for difenoconazole, 74.68-87.72% for trifloxystrobin and 78.59-92.66% for trifloxystrobin acid with relative standard deviations of 1.34-14.04%. The dissipation dynamics of difenoconazole and trifloxystrobin in watermelon and soil followed the first-order kinetics with half-lives of 3.2-8.8 days in both locations. The final residue levels of difenoconazole and trifloxystrobin were below 0.1 mg/kg (maximum residue level [MRL] set by China) and 0.2 mg/kg (MRL set by European Union), respectively, in pulp samples collected 14 days after the last application. These results could help Chinese authorities to establish MRL of trifloxystrobin in watermelon and provide guidance for the safe and proper application of both fungicides on watermelon.
[Mh] Termos MeSH primário: Acetatos/análise
Citrullus/química
Dioxolanos/análise
Fungicidas Industriais/análise
Cromatografia Gasosa-Espectrometria de Massas/métodos
Iminas/análise
Resíduos de Praguicidas/análise
Triazóis/análise
[Mh] Termos MeSH secundário: Limite de Detecção
Modelos Lineares
Metacrilatos/análise
Reprodutibilidade dos Testes
Estrobilurinas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Dioxolanes); 0 (Fungicides, Industrial); 0 (Imines); 0 (Methacrylates); 0 (Pesticide Residues); 0 (Strobilurins); 0 (Triazoles); D9612XCH4P (difenoconazole); F625Z36B2D (trifloxystrobin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170413
[St] Status:MEDLINE
[do] DOI:10.1002/bmc.3987



página 1 de 134 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde