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Pesquisa : D03.383.312.600 [Categoria DeCS]
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  1 / 3119 MEDLINE  
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[PMID]:29293328
[Au] Autor:Bhalsod GD; Chuang YH; Jeon S; Gui W; Li H; Ryser ET; Guber AK; Zhang W
[Ad] Endereço:Cook County Unit, University of Illinois Extension , Arlington Heights, Illinois 60004, United States.
[Ti] Título:Uptake and Accumulation of Pharmaceuticals in Overhead- and Surface-Irrigated Greenhouse Lettuce.
[So] Source:J Agric Food Chem;66(4):822-830, 2018 Jan 31.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Understanding the uptake and accumulation of pharmaceuticals in vegetables under typical irrigation practices is critical to risk assessment of crop irrigation with reclaimed water. This study investigated the pharmaceutical residues in greenhouse lettuce under overhead and soil-surface irrigations using pharmaceutical-contaminated water. Compared to soil-surface irrigation, overhead irrigation substantially increased the pharmaceutical residues in lettuce shoots. The increased residue levels persisted even after washing for trimethoprim, monensin sodium, and tylosin, indicating their strong sorption to the shoots. The postwashing concentrations in fresh shoots varied from 0.05 ± 0.04 µg/kg for sulfadiazine to 345 ± 139 µg/kg for carbamazepine. Root concentration factors ranged from 0.04 ± 0.14 for tylosin to 19.2 ± 15.7 for sulfamethoxazole. Translocation factors in surface-irrigated lettuce were low for sulfamethoxalzole, trimethoprim, monensin sodium, and tylosin (0.07-0.15), but high for caffeine (4.28 ± 3.01) and carbamazepine (8.15 ± 2.87). Carbamazepine was persistent in soil and hyperaccumulated in shoots.
[Mh] Termos MeSH primário: Irrigação Agrícola/métodos
Resíduos de Drogas/análise
Alface/metabolismo
Preparações Farmacêuticas/metabolismo
Poluentes Químicos da Água/metabolismo
[Mh] Termos MeSH secundário: Carbamazepina/análise
Contaminação de Alimentos
Alface/química
Monensin/análise
Preparações Farmacêuticas/análise
Folhas de Planta/química
Raízes de Plantas/química
Solo/química
Poluentes do Solo/análise
Poluentes do Solo/metabolismo
Sulfametoxazol/análise
Trimetoprima/análise
Tilosina/análise
Verduras
Poluentes Químicos da Água/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pharmaceutical Preparations); 0 (Soil); 0 (Soil Pollutants); 0 (Water Pollutants, Chemical); 33CM23913M (Carbamazepine); 906O0YJ6ZP (Monensin); AN164J8Y0X (Trimethoprim); JE42381TNV (Sulfamethoxazole); YEF4JXN031 (Tylosin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04355


  2 / 3119 MEDLINE  
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[PMID]:28468971
[Au] Autor:Olson MR; Ulrich BJ; Hummel SA; Khan I; Meuris B; Cherukuri Y; Dent AL; Janga SC; Kaplan MH
[Ad] Endereço:Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202; olsonmr@iupui.edu mkaplan2@iupui.edu.
[Ti] Título:Paracrine IL-2 Is Required for Optimal Type 2 Effector Cytokine Production.
[So] Source:J Immunol;198(11):4352-4359, 2017 06 01.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:IL-2 is a pleiotropic cytokine that promotes the differentiation of Th cell subsets, including Th1, Th2, and Th9 cells, but it impairs the development of Th17 and T follicular helper cells. Although IL-2 is produced by all polarized Th subsets to some level, how it impacts cytokine production when effector T cells are restimulated is unknown. We show in this article that Golgi transport inhibitors (GTIs) blocked IL-9 production. Mechanistically, GTIs blocked secretion of IL-2 that normally feeds back in a paracrine manner to promote STAT5 activation and IL-9 production. IL-2 feedback had no effect on Th1- or Th17-signature cytokine production, but it promoted Th2- and Th9-associated cytokine expression. These data suggest that the use of GTIs results in an underestimation of the presence of type 2 cytokine-secreting cells and highlight IL-2 as a critical component in optimal cytokine production by Th2 and Th9 cells in vitro and in vivo.
[Mh] Termos MeSH primário: Citocinas/biossíntese
Interleucina-2/metabolismo
Interleucina-9/biossíntese
Comunicação Parácrina
Células Th2/imunologia
[Mh] Termos MeSH secundário: Animais
Brefeldina A/farmacologia
Diferenciação Celular
Citocinas/imunologia
Interleucina-2/secreção
Interleucina-9/antagonistas & inibidores
Interleucina-9/imunologia
Ativação Linfocitária
Camundongos
Camundongos Endogâmicos C57BL
Monensin/farmacologia
Inibidores da Síntese de Proteínas/farmacologia
Ionóforos de Próton/farmacologia
Fator de Transcrição STAT5/metabolismo
Células Th1/imunologia
Células Th17/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cytokines); 0 (Interleukin-2); 0 (Interleukin-9); 0 (Protein Synthesis Inhibitors); 0 (Proton Ionophores); 0 (STAT5 Transcription Factor); 20350-15-6 (Brefeldin A); 906O0YJ6ZP (Monensin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180127
[Lr] Data última revisão:
180127
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1601792


  3 / 3119 MEDLINE  
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[PMID]:28549691
[Au] Autor:Kim BY; Son Y; Choi J; Eo SK; Park YC; Kim K
[Ad] Endereço:Department of Pharmacology, Pusan National University-School of Medicine, Yangsan, Gyeongnam 50612, Republic of Korea.
[Ti] Título:27-Hydroxycholesterol upregulates the production of heat shock protein 60 of monocytic cells.
[So] Source:J Steroid Biochem Mol Biol;172:29-35, 2017 Sep.
[Is] ISSN:1879-1220
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Investigating differentially expressed proteins in a milieu rich in cholesterol oxidation products, we found via mass spectrometry-based proteomics that surface levels of heat shock protein 60 (HSP60) were upregulated on monocytic cells in the presence of 27-hydroxycholesterol (27OHChol). The elevated levels of cytoplasmic membrane HSP60 were verified via Western blot analysis and visualized by confocal microscopy. Treatment with 27OHChol also resulted in increased levels of cellular HSP60 without altering its transcription. Cholesterol, however, did not affect cell-surface levels and cellular amount of HSP60. GSK 2033, an LXR antagonist, inhibited expression of live X receptor α, but not of HSP60, induced by 27OHChol. Treatment with 27OHChol also resulted in increased release of HSP60 from monocytic cells, but the release was significantly reduced by inhibitors of endoplasmic reticulum-Golgi protein trafficking, brefeldin A and monensin. Results of the current study indicate that 27OHChol upregulates not only cell-surface and cellular levels of HSP60 but also its release from monocytic cells, thereby contributing to activation of the immune system.
[Mh] Termos MeSH primário: Chaperonina 60/genética
Hidroxicolesteróis/farmacocinética
Proteínas Mitocondriais/genética
Monócitos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Brefeldina A/farmacologia
Linhagem Celular
Membrana Celular/efeitos dos fármacos
Membrana Celular/imunologia
Membrana Celular/metabolismo
Chaperonina 60/agonistas
Chaperonina 60/metabolismo
Retículo Endoplasmático/efeitos dos fármacos
Retículo Endoplasmático/imunologia
Retículo Endoplasmático/metabolismo
Regulação da Expressão Gênica
Complexo de Golgi/efeitos dos fármacos
Complexo de Golgi/imunologia
Complexo de Golgi/metabolismo
Seres Humanos
Hidroxicolesteróis/metabolismo
Imunidade Celular
Receptores X do Fígado/antagonistas & inibidores
Receptores X do Fígado/genética
Receptores X do Fígado/metabolismo
Proteínas Mitocondriais/agonistas
Proteínas Mitocondriais/metabolismo
Monensin/farmacologia
Monócitos/citologia
Monócitos/imunologia
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Sulfonamidas/farmacologia
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2,4,6-trimethyl-N-((3'-(methylsulfonyl)-4-biphenylyl)methyl)-N-((5-(trifluoromethyl)-2-furanyl)methyl)benzenesulfonamide); 0 (Chaperonin 60); 0 (HSPD1 protein, human); 0 (Hydroxycholesterols); 0 (Liver X Receptors); 0 (Mitochondrial Proteins); 0 (Sulfonamides); 20350-15-6 (Brefeldin A); 6T2NA6P5SQ (27-hydroxycholesterol); 906O0YJ6ZP (Monensin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170528
[St] Status:MEDLINE


  4 / 3119 MEDLINE  
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[PMID]:28434981
[Au] Autor:Anvari S; Grimbergen A; Davis CM; Makedonas G
[Ad] Endereço:Division of Immunology/Allergy/Rheumatology, Texas Children's Hospital, USA; Baylor College of Medicine, Houston, TX, USA.
[Ti] Título:Protein transport inhibitors downregulate the expression of LAG-3 on regulatory T cells.
[So] Source:J Immunol Methods;447:47-51, 2017 Aug.
[Is] ISSN:1872-7905
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Modern immunologic studies demand increasing complexity because of a need to improve our understanding of the relationship between a cell's phenotype and its function. Regulatory T cells (Tregs) have been defined by a narrow set of phenotypic markers, however their actual functional capacity has not been determined at the single-cell level. Although the lymphocyte activation gene 3 (LAG-3; CD223) is a key marker for the identification of exhausted T cells, it may be useful also in resolving Treg subpopulations by indicating distinct functional breadths. Here we define the experimental conditions necessary for the optimal detection by flow cytometry of LAG-3 expression on activated Tregs. We stimulated human PBMCs with either PMA/ionomycin or Staphylococcal Enterotoxin B (SEB) and analyzed CD4+CD25+FoxP3+ Tregs for LAG-3 expression in concert with other Treg phenotypic markers. We prescribe a 24-hour stimulation period for the optimal detection of LAG-3 on Tregs. Furthermore, we determine LAG-3 protein expression on Tregs is compromised when the cells are treated with brefeldin A (BFA) and monensin. Therefore, the simultaneous assessment of Treg phenotype and function is complicated by the use of protein transport inhibitors.
[Mh] Termos MeSH primário: Antígenos CD/genética
Brefeldina A/farmacologia
Monensin/farmacologia
Linfócitos T Reguladores/efeitos dos fármacos
Linfócitos T Reguladores/imunologia
[Mh] Termos MeSH secundário: Antígenos CD/imunologia
Biomarcadores
Células Cultivadas
Regulação para Baixo
Enterotoxinas/farmacologia
Citometria de Fluxo
Seres Humanos
Imunofenotipagem
Ionomicina/farmacologia
Leucócitos Mononucleares/efeitos dos fármacos
Leucócitos Mononucleares/imunologia
Ativação Linfocitária
Transporte Proteico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD); 0 (Biomarkers); 0 (CD223 antigen); 0 (Enterotoxins); 20350-15-6 (Brefeldin A); 39424-53-8 (enterotoxin B, staphylococcal); 56092-81-0 (Ionomycin); 906O0YJ6ZP (Monensin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170425
[St] Status:MEDLINE


  5 / 3119 MEDLINE  
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[PMID]:28386903
[Au] Autor:Doydora SA; Sun P; Cabrera M; Mantripragada N; Rema J; Pavlostathis SG; Huang CH; Thompson A
[Ad] Endereço:Department of Crop and Soil Sciences, University of Georgia, 3111 Miller Plant Sciences Building, 120 Carlton Street, Athens, GA, 30602-7272, USA.
[Ti] Título:Long-term broiler litter amendments can alter the soil's capacity to sorb monensin.
[So] Source:Environ Sci Pollut Res Int;24(15):13466-13473, 2017 May.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Monensin is a common antiparasitic drug given to poultry that contaminates poultry manure and bedding material (broiler litter). As broiler litter is commonly applied to agricultural fields as fertilizer, monensin could be released beyond the farm if it is not retained or degraded in the soil. This study aimed to assess the impact of long-term surface application of broiler litter (i.e., 17 years) on the capacity of pasture soil to sorb monensin. The soils were exposed to a range of monensin concentrations (0.18 to 1.81 µmol L ), solution pH (pH 4-9), and temperatures (15, 25, and 35 °C) and monensin was measured as loss from solution (i.e., sorption). Soils receiving long-term litter applications were hypothesized to retain more monensin than unamended soils because they have higher organic matter concentrations. However, soils from broiler litter-amended fields sorbed 46% less monensin than soils from unamended fields, likely because broiler litter also increased soil pH. The sorption of monensin to soil was strongly influenced by pH, with an order of magnitude greater sorption at pH 4 than at pH 9. Both soils had similar capacity to sorb monensin under similar solution pH, despite differences in organic carbon content (with the broiler litter-amended having 25% greater relative to the unamended soil). Temperature did not significantly impact monensin sorption for either soil. Our findings suggest increasing soil pH, for instance through liming, could enhance mobility of monensin.
[Mh] Termos MeSH primário: Esterco
Solo
[Mh] Termos MeSH secundário: Animais
Galinhas
Monensin
Poluentes do Solo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Manure); 0 (Soil); 0 (Soil Pollutants); 906O0YJ6ZP (Monensin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170408
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-017-8727-9


  6 / 3119 MEDLINE  
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[PMID]:28386840
[Au] Autor:Carvalho VV; Paulino MF; Detmann E; Chizzotti ML; Martins LS; Silva AG; Lopes SA; Moura FH
[Ad] Endereço:Animal Science Department, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil. victor.carvalho@ufv.br.
[Ti] Título:Effects of supplements containing different additives on nutritional and productive performance of beef cattle grazing tropical grass.
[So] Source:Trop Anim Health Prod;49(5):983-988, 2017 Jun.
[Is] ISSN:1573-7438
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A grazing trial was carried out to evaluate the inclusion of three feed additives in supplements (crude protein, CP 230 g/kg dry matter, DM) on the performance, voluntary intake, and digestibility of beef heifers grazing Brachiaria decumbens (CP 81 and neutral detergent fiber, NDF 615 g/kg DM). Thirty-five Nellore heifers (21 months of age and 383 ± 6.29 kg of body weight, BW) were used in a completely randomized design. The treatments were as follows: no supplement (control); supplement fed at 1 kg/animal/day without additives (S); supplement with monensin (S + M); supplement with yeast culture (S + YC); and supplement with enzyme complex (S + EC). All of the supplemented heifers had greater (P < 0.1) average daily gain (∼0.186 kg/day) compared to the control treatment (0.014 kg/day). Average daily gain and final BW were similar (P > 0.1) among supplemented heifers. Monensin inclusion in the supplement decreased (P < 0.1) forage DM (expressed as g/kg BW) and NDF intake (expressed as kg/day and as g/kg BW). All of the feed additive inclusions decreased (P < 0.1) NDF digestibility. In conclusion, the heifers' performance was improved by concentrate supplementation. However, the inclusion of additives did not enhance this effect.
[Mh] Termos MeSH primário: Bovinos/fisiologia
Digestão/efeitos dos fármacos
Monensin/metabolismo
Fermento Seco/metabolismo
[Mh] Termos MeSH secundário: Ração Animal/análise
Fenômenos Fisiológicos da Nutrição Animal
Animais
Brachiaria
Bovinos/crescimento & desenvolvimento
Dieta/veterinária
Fibras na Dieta/metabolismo
Feminino
Herbivoria
Monensin/administração & dosagem
Nitrogênio/metabolismo
Distribuição Aleatória
Fermento Seco/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dietary Fiber); 906O0YJ6ZP (Monensin); N762921K75 (Nitrogen)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170408
[St] Status:MEDLINE
[do] DOI:10.1007/s11250-017-1286-8


  7 / 3119 MEDLINE  
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[PMID]:28366563
[Au] Autor:Bouhlel J; Ratel J; Abouelkaram S; Mercier F; Travel A; Baéza E; Jondreville C; Dervilly-Pinel G; Marchand P; Le Bizec B; Dubreil E; Mompelat S; Verdon E; Inthavong C; Guérin T; Rutledge DN; Engel E
[Ad] Endereço:INRA, UR370 QuaPA, MASS laboratory, Saint-Genès-Champanelle, France; INRA, UMR GENIAL, AgroParisTech, Université Paris-Saclay, Massy, France.
[Ti] Título:Solid-phase microextraction set-up for the analysis of liver volatolome to detect livestock exposure to micropollutants.
[So] Source:J Chromatogr A;1497:9-18, 2017 May 12.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Starting from a critical analysis of a first "proof of concept" study on the utility of the liver volatolome for detecting livestock exposure to environmental micropollutants (Berge et al., 2011), the primary aim of this paper is to improve extraction conditions so as to obtain more representative extracts by using an extraction temperature closer to livestock physiological conditions while minimizing analytical variability and maximizing Volatile Organic Compound (VOC) abundancies. Levers related to extraction conditions and sample preparation were assessed in the light of both abundance and coefficient of variation of 22 candidate VOC markers identified in earlier volatolomic studies. Starting with a CAR/PDMS fiber and a 30min extraction, the reduction of SPME temperature to 40°C resulted in a significant decrease in the area of 14 candidate VOC markers (p<0.05), mainly carbonyls and alcohols but also a reduction in the coefficient of variation for 17 of them. In order to restore VOC abundances and to minimize variability, two approaches dealing with sample preparation were investigated. By increasing sample defrosting time at 4°C from 0 to 24h yielded higher abundances and lower variabilities for 15 and 13 compounds, respectively. Lastly, by using additives favouring the release of VOCs (1.2g of NaCl) the sensitivity of the analysis was improved with a significant increase in VOC abundances of more than 50% for 13 out of the 22 candidate markers. The modified SPME parameters significantly enhanced the abundances while decreasing the analytical variability for most candidate VOC markers. The second step was to validate the ability of the revised SPME protocol to discriminate intentionally contaminated broiler chickens from controls, under case/control animal testing conditions. After verification of the contamination levels of the animals by national reference laboratories, data analysis by a multivariate chemometric method (Common Components and Specific Weights Analysis - ComDim) showed that the liver volatolome could reveal dietary exposure of broilers to a group of environmental pollutants (PCBs), a veterinary treatment (monensin), and a pesticide (deltamethrin), thus confirming the usefulness of this analytical set-up.
[Mh] Termos MeSH primário: Poluentes Ambientais/análise
Fígado/química
Gado/metabolismo
Microextração em Fase Sólida/métodos
Compostos Orgânicos Voláteis/análise
[Mh] Termos MeSH secundário: Álcoois/análise
Animais
Galinhas/metabolismo
Monensin/análise
Nitrilos/análise
Praguicidas/análise
Piretrinas/análise
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alcohols); 0 (Environmental Pollutants); 0 (Nitriles); 0 (Pesticides); 0 (Pyrethrins); 0 (Volatile Organic Compounds); 2JTS8R821G (decamethrin); 906O0YJ6ZP (Monensin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170509
[Lr] Data última revisão:
170509
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE


  8 / 3119 MEDLINE  
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[PMID]:28352944
[Au] Autor:Thabet A; Honscha W; Daugschies A; Bangoura B
[Ad] Endereço:Institute of Parasitology, Faculty of Veterinary Medicine, Centre for Infectious Diseases, University of Leipzig, An den Tierkliniken 35, 04103, Leipzig, Germany.
[Ti] Título:Quantitative proteomic studies in resistance mechanisms of Eimeria tenella against polyether ionophores.
[So] Source:Parasitol Res;116(5):1553-1559, 2017 May.
[Is] ISSN:1432-1955
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Polyether ionophores are widely used to treat and control coccidiosis in chickens. Widespread use of anticoccidials resulted in worldwide resistance. Mechanisms of resistance development and expansion are complex and poorly understood. Relative proteomic quantification using LC-MS/MS was used to compare sensitive reference strains (Ref-1, Ref-2) with putatively resistant and moderately sensitive field strains (FS-R, FS-mS) of Eimeria tenella after isotopic labelling with tandem mass tags (TMT). Ninety-seven proteins were identified, and 25 of them were regulated. Actin was significantly upregulated in resistant strains in comparison with their sensitive counterparts. On the other hand, microneme protein (MIC4) was downregulated in resistant strains. Optimization of labelling E. tenella sporozoites by TMT might identify further proteins that play a role in the obvious complex mechanism leading to resistance against Monensin.
[Mh] Termos MeSH primário: Galinhas/parasitologia
Coccidiose/tratamento farmacológico
Coccidiose/veterinária
Coccidiostáticos/farmacologia
Eimeria tenella/efeitos dos fármacos
Ionóforos/farmacologia
Doenças das Aves Domésticas/tratamento farmacológico
[Mh] Termos MeSH secundário: Actinas/biossíntese
Animais
Resistência a Medicamentos
Monensin/farmacologia
Proteômica
Proteínas de Protozoários/biossíntese
Esporozoítos/efeitos dos fármacos
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins); 0 (Coccidiostats); 0 (Ionophores); 0 (Protozoan Proteins); 906O0YJ6ZP (Monensin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171007
[Lr] Data última revisão:
171007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170330
[St] Status:MEDLINE
[do] DOI:10.1007/s00436-017-5432-z


  9 / 3119 MEDLINE  
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[PMID]:28215897
[Au] Autor:Drong C; Bühler S; Frahm J; Hüther L; Meyer U; von Soosten D; Gessner DK; Eder K; Sauerwein H; Dänicke S
[Ad] Endereço:Institute of Animal Nutrition, Friedrich-Loeffler-Institut (FLI), Federal Research Institute for Animal Health, Bundesallee 50, 38116 Braunschweig, Germany.
[Ti] Título:Effects of body condition, monensin, and essential oils on ruminal lipopolysaccharide concentration, inflammatory markers, and endoplasmatic reticulum stress of transition dairy cows.
[So] Source:J Dairy Sci;100(4):2751-2764, 2017 Apr.
[Is] ISSN:1525-3198
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Evidence exists that dairy cows experience inflammatory-like phenomena in the transition period. Rumen health and alterations in metabolic processes and gene networks in the liver as the central metabolic organ might be key factors for cows' health and productivity in early lactation. This study made use of an animal model to generate experimental groups with different manifestations of postpartal fat mobilization and ketogenesis. In total, 60 German Holstein cows were allocated 6 wk antepartum to 3 high-body condition score (BCS) groups (BCS 3.95) and 1 low-BCS group (LC; BCS 2.77). High-BCS cows were fed an antepartal forage-to-concentrate ratio of 40:60 on dry matter basis, in contrast to 80:20 in the LC group, and received a monensin controlled-release capsule (HC/MO), a blend of essential oils (HC/EO), or formed a control group (HC). We evaluated serum haptoglobin, kynurenine, tryptophan, ruminal lipopolysaccharide concentration and mRNA abundance of nuclear factor kappa B (NF-κB), nuclear factor E2-related factor 2 (Nrf2), and endoplasmatic reticulum stress-induced unfolded protein response (UPR) target genes in liver biopsy samples from d -42 until +56 relative to calving. Nearly all parameters were highly dependent on time, with greatest variation near calving. The ruminal lipopolysaccharide concentration and evaluated target genes were not generally influenced by antepartal BCS and feeding management. The kynurenine-to-tryptophan ratio was higher in LC than in HC/MO treatment on d 7. Ruminal lipopolysaccharide concentration was higher in HC/MO than in the HC group, but not increased in HC/EO group. Abundance of UPR target gene X-box binding protein 1 was higher in HC/MO than in HC/EO group on d 7. Hepatic mRNA abundance of Nrf2 target gene glutathione peroxidase 3 was higher, whereas expression of NF-κB target gene haptoglobin tended to be higher in LC than in HC/EO cows. The HC/MO cows showed the most prominent increase in the abundance of glutathione peroxidase 3 and haptoglobin after calving in comparison to antepartal values. Results indicate the presence of inflammatory-like phenomena near calving. Simultaneously, alterations in UPR and Nrf2 target genes with antioxidative properties and haptoglobin occurred, being most prominent in LC and HC/MO group.
[Mh] Termos MeSH primário: Monensin/farmacologia
Óleos Voláteis/farmacologia
[Mh] Termos MeSH secundário: Animais
Bovinos
Dieta/veterinária
Feminino
Lactação
Leite/química
Retículo
Rúmen/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oils, Volatile); 906O0YJ6ZP (Monensin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170518
[Lr] Data última revisão:
170518
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170221
[St] Status:MEDLINE


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[PMID]:28161182
[Au] Autor:Schären M; Drong C; Kiri K; Riede S; Gardener M; Meyer U; Hummel J; Urich T; Breves G; Dänicke S
[Ad] Endereço:Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, Bundesallee 50, 38116 Brunswick, Germany.
[Ti] Título:Differential effects of monensin and a blend of essential oils on rumen microbiota composition of transition dairy cows.
[So] Source:J Dairy Sci;100(4):2765-2783, 2017 Apr.
[Is] ISSN:1525-3198
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In response to oral application, monensin alters the rumen microbiota, increasing ruminal propionate production and energy availability in the animal. Data from different studies indicate that the susceptibility of rumen bacteria to monensin is mainly cell-wall dependent but tracing its activity to specific microbial groups has been challenging. Several studies have shown a similar effect for essential oils but results are inconsistent. To investigate the influence of monensin and a blend of essential oils (BEO, containing thymol, guaiacol, eugenol, vanillin, salicylaldehyde, and limonene) on the rumen microbiome, rumen liquid samples were collected orally on d 56 postpartum from cows that had either received a monensin controlled-release capsule 3 wk antepartum, a diet containing a BEO from 3 wk antepartum onward, or a control diet (n = 12). The samples were analyzed for pH, volatile fatty acid, ammonia, and lipopolysaccharide concentrations and protozoal counts. A 16S rRNA gene fingerprinting analysis (PCR-single-strand conformation polymorphism) and sequencing revealed that the BEO treatment had no effect on the rumen microbiota, whereas monensin decreased bacterial diversity. Twenty-three bacterial species-level operational taxonomic units were identified for which monensin caused a significant decrease in their relative abundance, all belonging to the phyla Bacteroidetes (uncultured BS11 gut group and BS9 gut group) and Firmicutes (Lachnospiraceae, Ruminococcaceae, and Erysipelotrichaceae). Ten bacterial operational taxonomic units belonging to the phyla Actinobacteria (Coriobacteriaceae), Bacteroidetes (Prevotella), Cyanobacteria (SHA-109), and Firmicutes (Lachnospiraceae and Ruminococcaceae) increased in relative abundance due to the monensin treatment. These results confirm the hypothesis that varying effects depending on cell-wall constitution and thickness might apply for monensin sensitivity rather than a clear-cut difference between gram-negative and gram-positive bacteria. No effect of monensin on the archaea population was observed, confirming the assumption that reported inhibition of methanogenesis is most likely caused through a decrease in substrate availability, rather than by a direct effect on the methanogens. The data support the hypothesis that the observed increase in ruminal molar propionate proportions due to monensin may be caused by a decrease in abundance of non-producers and moderate producers of propionate and an increase in abundance of succinate and propionate producers.
[Mh] Termos MeSH primário: Monensin/farmacologia
Rúmen/metabolismo
[Mh] Termos MeSH secundário: Animais
Bovinos
Dieta/veterinária
Feminino
Fermentação
Microbiota/efeitos dos fármacos
Óleos Voláteis/farmacologia
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oils, Volatile); 0 (RNA, Ribosomal, 16S); 906O0YJ6ZP (Monensin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170518
[Lr] Data última revisão:
170518
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170206
[St] Status:MEDLINE



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