Base de dados : MEDLINE
Pesquisa : D03.383.533 [Categoria DeCS]
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[PMID]:28841529
[Au] Autor:Ghelichpour M; Taheri Mirghaed A; Mirzargar SS; Joshaghani H; Ebrahimzadeh Mousavi H
[Ad] Endereço:Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
[Ti] Título:Modification of saltwater stress response in Cyprinus carpio (Linnaeus, 1758) pre-exposed to pesticide indoxacarb.
[So] Source:Ecotoxicol Environ Saf;147:139-143, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:To evaluate the effects of indoxacarb on saltwater stress response in Cyprinus carpio, the fish were pre-exposed to indoxacarb (0, 0.75, 1.5 and 3mg/L denoted as CP, 0.75IT, 1.5IT and 3IT, respectively) for 21 days and then released to saltwater. A negative control (CN) group was included (the fish were held in indoxacarb-free water for the entire experiment). The fish were sampled immediately (0h) and 24, 48 and 72h after the salinity exposure for the analysis of plasma cortisol, glucose and sodium, chloride, potassium and calcium levels. All fish pre-exposed to 3mg/L indoxacarb, died after the first day of salinity challenge. CP showed typical cortisol response after the salinity challenge, but, cortisol response of the fish pre-exposed to indoxacarb (0.75IT and 1.5IT) was blocked. Plasma glucose increased significantly in all groups compared to the CN; however, this elevation had no consistent trend in 0.75IT and 1.5IT which indicated interference in glucose response due to indoxacarb exposure. Plasma sodium increased (compared to CN) in all groups after the salinity challenge. However, elevation in plasma chloride and potassium was significantly different among the groups and the indoxacarb-treated fish showed slightly sooner ionic disturbance. The results clearly indicate that indoxacarb impairs stress response of C. carpio and the fish may not be able to respond normally to additional stressors, which threatens their survival.
[Mh] Termos MeSH primário: Carpas/metabolismo
Hidrocortisona/sangue
Osmorregulação/efeitos dos fármacos
Oxazinas/toxicidade
Praguicidas/toxicidade
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Carpas/sangue
Cloretos/sangue
Relação Dose-Resposta a Droga
Potássio/sangue
Salinidade
Sódio/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chlorides); 0 (Oxazines); 0 (Pesticides); 0 (Water Pollutants, Chemical); 52H0D26MWR (indoxacarb); 9NEZ333N27 (Sodium); RWP5GA015D (Potassium); WI4X0X7BPJ (Hydrocortisone)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170826
[St] Status:MEDLINE


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[PMID]:28942278
[Au] Autor:Demirci Ö; Güven K; Asma D; Ögüt S; Ugurlu P
[Ad] Endereço:Science Faculty, Department of Biology, Dicle University, 21280, Turkey. Electronic address: ozdem22@gmail.com.
[Ti] Título:Effects of endosulfan, thiamethoxam, and indoxacarb in combination with atrazine on multi-biomarkers in Gammarus kischineffensis.
[So] Source:Ecotoxicol Environ Saf;147:749-758, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Studies addressing the toxicity of pesticides towards non-target organisms focus on the median lethal concentration and biochemical response of individual pesticides. However, when determining environmental risks, it is important to test the combined effects of pesticides, such as insecticides and herbicides, which are frequently used together in agricultural areas. Here we aimed to investigate the toxic effects of the combined use of the herbicide atrazine and the insecticides, endosulfan, indoxacarb, and thiamethoxam on Gammarus kischineffensis. To do this, we tested the activities of oxidative stress, detoxification, and neurotoxicity biomarkers. Compared to atrazine alone, we detected higher glutathione-S-transferase, catalase and superoxide dismutase activities (oxidative stress biomarkers) when atrazine was combined with either endosulfan or indoxacarb. However, higher IBR values were determined in organisms where pesticide mixtures were used according to individual use. Based on these results, mixtures of atrazine and other pesticides may cause synergistic effects and may be evidence of increased toxicity and oxidative stress.
[Mh] Termos MeSH primário: Anfípodes/efeitos dos fármacos
Monitoramento Ambiental/métodos
Herbicidas/toxicidade
Inseticidas/toxicidade
Poluentes do Solo/toxicidade
[Mh] Termos MeSH secundário: Anfípodes/enzimologia
Animais
Atrazina/toxicidade
Biomarcadores/análise
Relação Dose-Resposta a Droga
Sinergismo Farmacológico
Endossulfano/toxicidade
Dose Letal Mediana
Neonicotinoides/toxicidade
Nitrocompostos/toxicidade
Oxazinas/toxicidade
Estresse Oxidativo/efeitos dos fármacos
Tiazóis/toxicidade
Fatores de Tempo
Testes de Toxicidade Aguda
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Herbicides); 0 (Insecticides); 0 (Neonicotinoids); 0 (Nitro Compounds); 0 (Oxazines); 0 (Soil Pollutants); 0 (Thiazoles); 52H0D26MWR (indoxacarb); 747IC8B487 (thiamethoxam); OKA6A6ZD4K (Endosulfan); QJA9M5H4IM (Atrazine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170925
[St] Status:MEDLINE


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[PMID]:29318312
[Au] Autor:Sharma V; Jaiswal PK; Yadav DK; Saran M; Prikhodko J; Mathur M; Swami AK; Mashevskaya IV; Chaudhary S
[Ti] Título:Microwave-assisted One-pot Efficient Synthesis of Functionalized 2-Oxo-2-phenylethylidenes-linked 2-Oxobenzo[1,4]oxazines and 2-Oxoquino[1,4]oxalines: Synthetic Applications, Antioxidant Activity, SAR and Cytotoxic Studies.
[So] Source:Acta Chim Slov;64(4):988-1004, 2017 Dec.
[Is] ISSN:1318-0207
[Cp] País de publicação:Slovenia
[La] Idioma:eng
[Ab] Resumo:A microwave-assisted, environmentally benign green protocol for the synthesis of functionalized (Z)-3-(2-oxo-2-phenylethylidene)-3, 4-dihydro-2H-benzo[b][1,4]oxazin-2-ones (11a-n) in excellent yields (upto 97%) and (Z)-3-(2-oxo-2-phenylethylidene)-3,4-dihydroquinoxalin-2(1H)-ones (14a-h) (upto 96% yield) are reported. The practical applicability of developed methodology were also confirmed by the gram scale synthesis of 11a, 14c and 14e; synthesis of anticancer alkaloid Cephalandole A 16 (89% yield). All the synthesized compounds 11a-n, 14a-h and 16 were assessed for their in vitro antioxidant activities in DPPH radical scavenging and FRAP assay. In DPPH assay, compounds 11a, 14c and 14e, the most active compounds of the series, were found to show IC50 value of 10.20 ± 0.08 µg/mL, 9.89 ± 0.15 µg/mL and 8.97 ± 0.13 µg/mL, respectively in comparison with standard reference (ascorbic acid, IC50 = 4.57 µg/mL). Whereas, in FRAP antioxidant assay seven compounds (11c, 11e, 11i, 11k, 11l, 14d and 14h) displayed higher antioxidant activity in comparison to the reference standard BHT (C0.5FRAP = 546.2 µM). Moreover, the cytotoxic studies of the compounds 11a, 14c, 14e and 14h were found to be non-toxic in nature in 3T3 fibroblast cell lines using MTT assay.
[Mh] Termos MeSH primário: Antineoplásicos/síntese química
Antioxidantes/síntese química
Oxazinas/síntese química
Quinoxalinas/síntese química
[Mh] Termos MeSH secundário: Células 3T3
Animais
Antineoplásicos/farmacologia
Antioxidantes/farmacologia
Camundongos
Micro-Ondas
Oxazinas/farmacologia
Quinoxalinas/farmacologia
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Antioxidants); 0 (Oxazines); 0 (Quinoxalines)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE


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[PMID]:29231221
[Au] Autor:Cozzoli L; Gjonaj L; Stuart MCA; Poolman B; Roelfes G
[Ad] Endereço:Stratingh Institute for Chemistry, Nijenborgh 4, 9747 AG Groningen, The Netherlands. j.g.roelfes@rug.nl.
[Ti] Título:Responsive DNA G-quadruplex micelles.
[So] Source:Chem Commun (Camb);54(3):260-263, 2018 Jan 02.
[Is] ISSN:1364-548X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel and versatile design of DNA-lipid conjugates is presented. The assembly of the DNA headgroups into G-quadruplex structures is essential for the formation of micelles and their stability. By hybridization with a complementary oligonucleotide the micelles were destabilized, resulting in cargo release. In combination with a hairpin DNA aptamer as complementary strand, the release is obtained selectively by the presence of ATP.
[Mh] Termos MeSH primário: DNA/química
Portadores de Fármacos/química
Quadruplex G
Lipídeos/química
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/química
Animais
Aptâmeros de Nucleotídeos/genética
Carbocianinas/química
Bovinos
DNA/genética
Liberação Controlada de Fármacos
Corantes Fluorescentes/química
Micelas
Hibridização de Ácido Nucleico
Oxazinas/química
Soroalbumina Bovina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aptamers, Nucleotide); 0 (Carbocyanines); 0 (Drug Carriers); 0 (Fluorescent Dyes); 0 (Lipids); 0 (Micelles); 0 (Oxazines); 27432CM55Q (Serum Albumin, Bovine); 40957-95-7 (3,3'-dioctadecylindocarbocyanine); 68006-80-4 (3,3'-dioctadecyloxacarbocyanine); 8L70Q75FXE (Adenosine Triphosphate); 9007-49-2 (DNA); P476F1L81G (nile red)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.1039/c7cc07899d


  5 / 6869 MEDLINE  
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[PMID]:28470516
[Au] Autor:Ivanov DP; Grabowska AM; Garnett MC
[Ad] Endereço:Cancer Biology, Division of Cancer and Stem Cells, School of Medicine, Queen's Medical Centre, University of Nottingham, Nottingham, NG7 2UH, UK. delyan.ivanov@nottingham.ac.uk.
[Ti] Título:High-Throughput Spheroid Screens Using Volume, Resazurin Reduction, and Acid Phosphatase Activity.
[So] Source:Methods Mol Biol;1601:43-59, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mainstream adoption of physiologically relevant three-dimensional models has been slow in the last 50 years due to long, manual protocols with poor reproducibility, high price, and closed commercial platforms. This chapter describes high-throughput, low-cost, open methods for spheroid viability assessment which use readily available reagents and open-source software to analyze spheroid volume, metabolism, and enzymatic activity. We provide two ImageJ macros for automated spheroid size determination-for both single images and images in stacks. We also share an Excel template spreadsheet allowing users to rapidly process spheroid size data, analyze plate uniformity (such as edge effects and systematic seeding errors), detect outliers, and calculate dose-response. The methods would be useful to researchers in preclinical and translational research planning to move away from simplistic monolayer studies and explore 3D spheroid screens for drug safety and efficacy without substantial investment in money or time.
[Mh] Termos MeSH primário: Sobrevivência Celular
Ensaios de Triagem em Larga Escala/métodos
Indicadores e Reagentes/metabolismo
Esferoides Celulares/fisiologia
[Mh] Termos MeSH secundário: Fosfatase Ácida/metabolismo
Encéfalo/citologia
Linhagem Celular Tumoral
Relação Dose-Resposta a Droga
Avaliação Pré-Clínica de Medicamentos
Ensaios de Triagem em Larga Escala/economia
Seres Humanos
Processamento de Imagem Assistida por Computador
Oxazinas/química
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Software
Esferoides Celulares/citologia
Esferoides Celulares/metabolismo
Fatores de Tempo
Xantenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indicators and Reagents); 0 (Oxazines); 0 (Xanthenes); 1FN9YD6968 (resazurin); EC 3.1.3.2 (Acid Phosphatase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6960-9_4


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[PMID]:28470513
[Au] Autor:Präbst K; Engelhardt H; Ringgeler S; Hübner H
[Ad] Endereço:Institute of Bioprocess Engineering, Friedrich-Alexander University Erlangen-Nürnberg, Paul-Gordan-Str. 3, 91052, Erlangen, Germany. konstantin.praebst@fau.de.
[Ti] Título:Basic Colorimetric Proliferation Assays: MTT, WST, and Resazurin.
[So] Source:Methods Mol Biol;1601:1-17, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This chapter describes selected assays for the evaluation of cellular viability and proliferation of cell cultures. The underlying principle of these assays is the measurement of a biochemical marker to evaluate the cell's metabolic activity. The formation of the omnipresent reducing agents NADH and NADPH is used as a marker for metabolic activity in the following assays. Using NADH and NADPH as electron sources, specific dyes are biochemically reduced which results in a color change that can be determined with basic photometrical methods. The assays selected for this chapter include MTT, WST, and resazurin. They are applicable for adherent or suspended cell lines, easy to perform, and comparably economical. Detailed protocols and notes for easier handling and avoiding pitfalls are enclosed to each assay.
[Mh] Termos MeSH primário: Contagem de Células/métodos
Proliferação Celular
Sobrevivência Celular
Colorimetria/métodos
Indicadores e Reagentes/química
Oxazinas/química
Sais de Tetrazólio/química
Tiazóis/química
Xantenos/química
[Mh] Termos MeSH secundário: Bioensaio
Calibragem
Células HeLa
Seres Humanos
NAD/análise
NADP/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium monosodium salt); 0 (Indicators and Reagents); 0 (Oxazines); 0 (Tetrazolium Salts); 0 (Thiazoles); 0 (Xanthenes); 0U46U6E8UK (NAD); 1FN9YD6968 (resazurin); 53-59-8 (NADP); EUY85H477I (thiazolyl blue)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6960-9_1


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[PMID]:29262703
[Au] Autor:Gyori J; Farkas A; Stolyar O; Székács A; Mörtl M; Vehovszky Á
[Ad] Endereço:1 Department of Experimental Zoology, MTA Centre for Ecological Research, Balaton Limnological Institute , H-8237 Tihany, POB 35 , Hungary.
[Ti] Título:Inhibitory effects of four neonicotinoid active ingredients on acetylcholine esterase activity.
[So] Source:Acta Biol Hung;68(4):345-357, 2017 Dec.
[Is] ISSN:0236-5383
[Cp] País de publicação:Hungary
[La] Idioma:eng
[Ab] Resumo:There is a great concern about the decline of pollinators, and neonicotinoids emerging bee disorders are assumed to play a significant role. Since changes in learning ability has been observed in honey bees exposed to some acetylcholine esterase (AChE) inhibitors, we therefore, tested in vitro the effect of four neonicotinoids on purified eel AChE. AChE activity was inhibited in a concentration-dependent manner, and calculated IC values for thiamethoxam (IC = 414 µM) and clothianidin (IC = 160 µM) were found to be much higher compared to acetamiprid (IC = 75.2 µM) and thiacloprid (IC = 87.8 µM). The Lineweaver-Burk reciprocal plots for acetamiprid shows unchanged V and increased K values with inhibitor concentrations, while analysis of Michaelis-Menten plots shows predominantly competitive mechanism. The inhibition constant value (K = 24.3 µM) indicates strong binding of the acetamiprid complex to AChE. Finally, the four tested neonicotinoids are not a uniform group regarding their blocking ability. Our results suggest a previously not established, direct AChE blocking mechanism of neonicotinoids tested, thus the neuronal AChE enzyme is likely among the direct targets of the neonicotinoid insecticides. We conclude, that these AChE inhibitory effects may also contribute to toxic effects on the whole exposed animal.
[Mh] Termos MeSH primário: Acetilcolinesterase/química
Inibidores da Colinesterase/química
Electrophorus
Proteínas de Peixes
Guanidinas/química
Neonicotinoides/química
Nitrocompostos/química
Oxazinas/química
Tiazóis/química
[Mh] Termos MeSH secundário: Animais
Proteínas de Peixes/antagonistas & inibidores
Proteínas de Peixes/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cholinesterase Inhibitors); 0 (Fish Proteins); 0 (Guanidines); 0 (Neonicotinoids); 0 (Nitro Compounds); 0 (Oxazines); 0 (Thiazoles); 2V9906ABKQ (clothianidin); 5HL5N372P0 (acetamiprid); 747IC8B487 (thiamethoxam); EC 3.1.1.7 (Acetylcholinesterase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1556/018.68.2017.4.1


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[PMID]:27776597
[Au] Autor:Pina RZ; Caleffi-Ferracioli KR; Campanerut-Sá PA; Ghiraldi-Lopez LD; Pavan FR; Siqueira VL; Scodro RB; Cardoso RF
[Ad] Endereço:Post-Graduate Programme in Health Sciences, State University of Maringá, Maringá, Brazil.
[Ti] Título:Pyrazinamide susceptibility testing in Mycobacterium tuberculosis using the fast resazurin microtiter assay plate.
[So] Source:Int J Tuberc Lung Dis;20(11):1535-1538, 2016 Nov.
[Is] ISSN:1815-7920
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:SETTING: Department of Clinical Analysis and Biomedicine, State University of Maringa, Maringa, PR, Brazil. OBJECTIVE: To evaluate the performance of the resazurin microtiter assay (REMA) plate at pH 5.5 in detecting Mycobacterium tuberculosis susceptibility to pyrazinamide (PZA). DESIGN: The minimal inhibitory concentration (MIC) of PZA in M. tuberculosis H Rv and M. bovis AN5 reference strains and in 34 clinical M. tuberculosis isolates (26 PZA-susceptible and eight PZA-resistant) was determined using REMA at pH 5.5 and compared to REMA at pH 6.0. RESULTS: REMA at pH 5.5 was helpful in discriminating PZA-susceptible from resistant M. tuberculosis isolates when â©¿50 µg/ml PZA was considered as the cut-off for PZA susceptibility. Furthermore, it provided results in 8 days. However, two PZA-resistant isolates failed to grow at pH 5.5. CONCLUSION: As the REMA method is rapid, inexpensive, easy to perform and read, it would be of great usefulness in low-income countries for detecting PZA-resistant M. tuberculosis. REMA at pH 5.6-5.9 should be evaluated on an extended panel of clinical M. tuberculosis isolates with a greater range of MIC values in different laboratories for a better understanding of its utility in differentiating PZA-resistant from PZA-susceptible isolates.
[Mh] Termos MeSH primário: Antituberculosos/farmacologia
Farmacorresistência Bacteriana
Testes de Sensibilidade Microbiana/métodos
Mycobacterium tuberculosis/efeitos dos fármacos
Pirazinamida/farmacologia
[Mh] Termos MeSH secundário: Brasil
Seres Humanos
Concentração de Íons de Hidrogênio
Oxazinas
Xantenos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antitubercular Agents); 0 (Oxazines); 0 (Xanthenes); 1FN9YD6968 (resazurin); 2KNI5N06TI (Pyrazinamide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


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[PMID]:29303272
[Au] Autor:Chai XH; Meng Z; Cao PR; Jiang J; Liu YF
[Ad] Endereço:State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University , 1800 Lihu Road, Wuxi 214122, Jiangsu, P
[Ti] Título:Comparative Analysis of Small-Molecule Diffusivity in Different Fat Crystal Network.
[So] Source:J Agric Food Chem;66(4):1015-1022, 2018 Jan 31.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Oil migration and fat recrystallization in fat-structured food materials can result in significant deterioration in food quality. Consequently, it is important to monitor and quantify the diffusivities of the migrants in fat crystal network. The diffusion coefficients of Nile red dye in liquid oils through fully hydrogenated palm kernel oil (FHPKO)/triolein (OOO) and fully hydrogenated soybean oil (FHSO)/triolein (OOO) systems were evaluated by the fluorescence recovery after photobleaching (FRAP) method. The effective diffusion coefficients (D ) and mobile fraction (M ) increased with the decrease of solid fat contents (SFC), with the changes of microstructure from more densely to slightly larger packed clusters for both FHPKO/OOO and FHSO/OOO systems. In addition, microstructural parameters of these systems were estimated by the image analysis. The results showed that the diffusion of dye and liquid oil was affected by the microstructure. The higher D was associated with lower fractal dimensions, larger crystal thickness, and larger average particle sizes. Finally, higher-permeability coefficients were calculated according to Darcy's Law, and it was significantly correlated to the D .
[Mh] Termos MeSH primário: Óleos Vegetais/química
[Mh] Termos MeSH secundário: Cristalização
Difusão
Recuperação de Fluorescência Após Fotodegradação
Corantes Fluorescentes
Microscopia Confocal
Oxazinas
Óleo de Palmeira/química
Óleo de Soja/química
Trioleína/química
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fluorescent Dyes); 0 (Oxazines); 0 (Plant Oils); 122-32-7 (Triolein); 5QUO05548Z (Palm Oil); 8001-22-7 (Soybean Oil); P476F1L81G (nile red)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04677


  10 / 6869 MEDLINE  
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[PMID]:28748329
[Au] Autor:Gajger IT; Sakac M; Gregorc A
[Ad] Endereço:Laboratory for Honeybee Diseases - NRL, Department for Biology and Pathology of Fish and Bees, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000, Zagreb, Croatia. ivana.tlak@vef.hr.
[Ti] Título:Impact of Thiamethoxam on Honey Bee Queen (Apis mellifera carnica) Reproductive Morphology and Physiology.
[So] Source:Bull Environ Contam Toxicol;99(3):297-302, 2017 Sep.
[Is] ISSN:1432-0800
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:High honey bee losses around the world have been linked in part by the regular use of neonicotinoids in agriculture. In light of the current situation, the aim of this study was to investigate the effects of thiamethoxam on the development of the reproductive system and physiology in the honey bee queen. Two experimental groups of honey bee queen larvae were treated with thiamethoxam during artificial rearing, applied via artificial feed in two cycles. In the first rearing cycle, honey bee larvae received a single treatment dose (4.28 ng thiamethoxam/queen larva on the 4th day after larvae grafting in artificial queen cells), while the second honey bee queen rearing cycle received a double treatment dose (total of 8.56 ng thiamethoxam/queen larva on the 4th and 5th day after larvae grafting in artificial queen cells). After emerging, queens were anesthetized and weighed, and after mating with drones were anesthetized, weighed, and sectioned. Ovary mass and number of stored sperm were determined. Body weight differed between untreated and treated honey bee queens. The results also show a decrease in the number of sperm within honey bee queen spermathecae that received the double thiamethoxam dose.
[Mh] Termos MeSH primário: Abelhas
Inseticidas/toxicidade
Neonicotinoides/toxicidade
Nitrocompostos/toxicidade
Oxazinas/toxicidade
Tiazóis/toxicidade
[Mh] Termos MeSH secundário: Animais
Feminino
Larva/efeitos dos fármacos
Masculino
Reprodução/efeitos dos fármacos
Espermatozoides/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insecticides); 0 (Neonicotinoids); 0 (Nitro Compounds); 0 (Oxazines); 0 (Thiazoles); 747IC8B487 (thiamethoxam)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1007/s00128-017-2144-0



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