Base de dados : MEDLINE
Pesquisa : D03.383.663.283.240.190 [Categoria DeCS]
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[PMID]:29373584
[Au] Autor:Zhao X; Li R; Jin H; Jin H; Wang Y; Zhang W; Wang H; Chen W
[Ad] Endereço:Tianjin Institute of Health and Environmental Medicine, Tianjin, China.
[Ti] Título:Epigallocatechin-3-gallate confers protection against corticosterone-induced neuron injuries via restoring extracellular signal-regulated kinase 1/2 and phosphatidylinositol-3 kinase/protein kinase B signaling pathways.
[So] Source:PLoS One;13(1):e0192083, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Extensive studies suggested epigallocatechin-3-gallate (EGCG) has significant neuroprotection against multiple central neural injuries, but the underlying mechanisms still remain poorly elucidated. Here we provide evidence to support the possible involvement of extracellular signal-regulated kinase 1/2 (ERK1/2) and phosphatidylinositol-3 kinase/ protein kinase B (PI3K/AKT) pathways in EGCG-mediated protection against corticosterone-induced neuron injuries. As an essential stress hormone, corticosterone could induce obvious neurotoxicity in primary hippocampal neurons. Pre-treatment with EGCG ameliorated the corticosterone-induced neuronal injuries; however, it was blocked by pharmacological inhibitors for ERK1/2 (U0126) and PI3K/AKT (LY294002). Furthermore, the results confirmed that EGCG restored the corticosterone-induced decrease of ERK1/2 and PI3K/AKT phosphorylation, and attenuated the corticosterone-induced reduction of peroxisome proliferators-activated receptor-γ coactivator-1α (PGC-1α) expression and ATP production. Taken together, these findings indicated that EGCG has significant neuroprotection against corticosterone-induced neuron injuries partly via restoring the ERK1/2 and PI3K/AKT signaling pathways as well as the PGC-1α-mediated ATP production.
[Mh] Termos MeSH primário: Catequina/análogos & derivados
Corticosterona/efeitos adversos
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Neurônios/efeitos dos fármacos
Fosfatidilinositol 3-Quinases/antagonistas & inibidores
Proteínas Proto-Oncogênicas c-akt/metabolismo
Transdução de Sinais
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/biossíntese
Animais
Catequina/farmacologia
Células Cultivadas
Hipocampo/citologia
Hipocampo/efeitos dos fármacos
Fármacos Neuroprotetores/farmacologia
Fosforilação
Ratos
Ratos Wistar
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Neuroprotective Agents); 8L70Q75FXE (Adenosine Triphosphate); 8R1V1STN48 (Catechin); BQM438CTEL (epigallocatechin gallate); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt); W980KJ009P (Corticosterone)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180127
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0192083


  2 / 8097 MEDLINE  
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[PMID]:29203747
[Au] Autor:Mielczarek-Puta M; Chrzanowska A; Otto-Slusarczyk D; Grabon W; Baranczyk-Kuzma A
[Ad] Endereço:Katedra I Zaklad Biochemii, Warszawski Uniwersytet Medyczny, Warszawa, Polska.
[Ti] Título:[Effect of antioxidants on human primary and metastatic colon cancer cells at hypoxia and normoxia].
[So] Source:Wiad Lek;70(5):946-952, 2017.
[Is] ISSN:0043-5147
[Cp] País de publicação:Poland
[La] Idioma:pol
[Ab] Resumo:THE AIM: Evaluation of some antioxidants on human colon cancer cells viability and proliferation at various oxygen levels. MATERIAL AND METHODS: Human primary (SW480) and metastatic (SW620) colon cancer cells were cultured at hypoxia (1% oxygen), tissues (10% oxygen) and atmospheric (21% oxygen) normoxia with quercetin, epigallocatechin gallate, lipoic acid, hydroxycitric acid, their mixture, and without studied compounds (control). Antioxidants were used at physiological concentrations. The cell viability was determined by trypan blue dye exclusion and proliferation by MTT assay. RESULTS: The viability of each line ranged from 80% to 97%, and it was independent on the compound and oxygen availability. At hypoxia the cell count of both lines was lower than for the controls in the presence of each studied compound. At tissue normoxia the cell count of primary cancer cells was decreased only with epigallocatechin gallate, whereas metastatic cells were sensitive for each antioxidant. CONCLUSIONS: Our results indicated, that the studied antioxidants were not cytotoxic at physiological levels for both pirmary and metastatic colon cancer. Their cytostatic effect depend on the type of cell, oxygen availability and antioxidant concentration.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Hipóxia Celular/efeitos dos fármacos
Neoplasias do Colo/tratamento farmacológico
[Mh] Termos MeSH secundário: Catequina/análogos & derivados
Catequina/farmacologia
Linhagem Celular Tumoral/efeitos dos fármacos
Citratos/farmacologia
Neoplasias do Colo/patologia
Seres Humanos
Metástase Neoplásica
Oxigênio/farmacologia
Ácido Tióctico/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Citrates); 73Y7P0K73Y (Thioctic Acid); 8R1V1STN48 (Catechin); 8W94T9026R (hydroxycitric acid); BQM438CTEL (epigallocatechin gallate); S88TT14065 (Oxygen)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE


  3 / 8097 MEDLINE  
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[PMID]:29197967
[Au] Autor:Allegri L; Rosignolo F; Mio C; Filetti S; Baldan F; Damante G
[Ad] Endereço:Department of Medical Area, University of Udine, 33100, Udine, Italy.
[Ti] Título:Effects of nutraceuticals on anaplastic thyroid cancer cells.
[So] Source:J Cancer Res Clin Oncol;144(2):285-294, 2018 Feb.
[Is] ISSN:1432-1335
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:PURPOSE: The anaplastic thyroid carcinoma (ATC) is the most aggressive thyroid cancer with a high mortality rate. Since nutraceuticals may exert beneficial effects on tumor biology, here, effects of four of these compounds [resveratrol, genistein, curcumin and epigallocatechin-3-gallate (EGCG)] on ATC cell lines were investigated. METHODS: Two ATC-derived cell lines were used: SW1736 and 8505C. Cell viability and in vitro aggressiveness was tested by MTT and soft agar assays. Apoptosis was investigated by Western Blot, using an anti-cleaved-PARP antibody. mRNA and miRNA levels were quantified by real-time PCR. RESULTS: All tested nutraceuticals caused in both cell lines decrease of cell viability and increase of apoptosis. In contrast, only curcumin reduced in vitro aggressiveness in both SW1736 and 8505C cell lines, while genistein and EGCG determined a reduction of colony formation only in 8505C cells. Effects on genes related to the thyroid-differentiated phenotype were also tested: resveratrol and genistein administration determined the increment of almost all tested mRNAs in both cell lines. Instead curcumin and EGCG treatments had opposite effects in the two cell lines, causing the increment of almost all the mRNAs in 8505C cells and their reduction in SW1736. Finally, effects of nutraceuticals on levels of several miRNAs, known as important in thyroid cancer progression (hsa-miR-221, hsa-miR-222, hsa-miR-21, hsa-miR-146b, hsa-miR-204), were tested. Curcumin induced a strong and significant reduction of all miR analyzed, except for has-miR-204, in both cell lines. CONCLUSIONS: Altogether, our results clearly indicate the anti-cancer proprieties of curcumin, suggesting the promising use of this nutraceutical in ATC treatment. Resveratrol, genistein and EGCG have heterogeneous effects on molecular features of ATC cells.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Suplementos Nutricionais
Carcinoma Anaplásico da Tireoide/tratamento farmacológico
Neoplasias da Glândula Tireoide/tratamento farmacológico
[Mh] Termos MeSH secundário: Apoptose/efeitos dos fármacos
Catequina/análogos & derivados
Catequina/farmacologia
Diferenciação Celular/efeitos dos fármacos
Processos de Crescimento Celular/efeitos dos fármacos
Linhagem Celular Tumoral
Curcumina/farmacologia
Genisteína/farmacologia
Seres Humanos
MicroRNAs/biossíntese
MicroRNAs/genética
Estilbenos/farmacologia
Carcinoma Anaplásico da Tireoide/genética
Carcinoma Anaplásico da Tireoide/patologia
Neoplasias da Glândula Tireoide/genética
Neoplasias da Glândula Tireoide/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (MicroRNAs); 0 (Stilbenes); 8R1V1STN48 (Catechin); BQM438CTEL (epigallocatechin gallate); DH2M523P0H (Genistein); IT942ZTH98 (Curcumin); Q369O8926L (resveratrol)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171204
[St] Status:MEDLINE
[do] DOI:10.1007/s00432-017-2555-7


  4 / 8097 MEDLINE  
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[PMID]:28465354
[Au] Autor:Farabegoli F; Govoni M; Spisni E; Papi A
[Ad] Endereço:Department of Pharmacology and Biotechnology (FaBiT), University of Bologna, Bologna, Italy fulvia.farabegoli@unibo.it.
[Ti] Título:EGFR inhibition by (-)-epigallocatechin-3-gallate and IIF treatments reduces breast cancer cell invasion.
[So] Source:Biosci Rep;37(3), 2017 Jun 30.
[Is] ISSN:1573-4935
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Epidermal growth factor receptor (EGFR) expression is an important marker in breast carcinoma pathology and is considered a pivotal molecule for cancer cell proliferation, invasion and metastasis. We investigated the effects of epigallocatechin-3-gallate (EGCG), the most active green tea catechin, in combination with 6-OH-11-O-hydroxyphenanthrene (IIF), a synthetic retinoid X receptor-γ (RXRγ) agonist, on three breast carcinoma cell lines: MCF-7, MCF-7TAM and MDA-MB-231. EGFR and AKT activation and molecular markers of cell motility and migration (CD44, extracellular matrix metalloproteinase (MMP) inducer (EMMPRIN), MMP-2, MMP-9 and tissue inhibitor of metalloproteinases (TIMPs)) were studied after EGCG and IIF treatments. The EGCG + IIF treatment was the most active in down-regulating EGFR phosphorylation at Tyr in all the investigated cell lines; p473AKT was also down-regulated in MCF-TAM cells. EGCG + IIF was also the most active treatment in reducing the expression of markers of invasion and migration in all the three cell lines: CD44, EMMPRIN, MMP-2 and -9 expression decreased, whereas TIMPs were up-regulated. Zymography and scratch assay also confirmed the reduced invasion tendency. We considered that EGCG and IIF treatments could alter the molecular network based on EGFR, CD44 and EMMPRIN expression interdependence and reduced the migration tendency in MCF-7, MCF-7TAM and MDA-MB-231 cells. These events only occurred in association with AKT inactivation in MCF-7TAM cells. In conclusion, the combination of EGCG and IIF significantly attenuated the invasive behaviour of breast carcinoma cells.
[Mh] Termos MeSH primário: Neoplasias da Mama/tratamento farmacológico
Catequina/análogos & derivados
Movimento Celular/efeitos dos fármacos
Invasividade Neoplásica/patologia
Fenantrenos/farmacologia
Receptor do Fator de Crescimento Epidérmico/antagonistas & inibidores
[Mh] Termos MeSH secundário: Basigina/metabolismo
Neoplasias da Mama/metabolismo
Neoplasias da Mama/patologia
Catequina/farmacologia
Linhagem Celular Tumoral
Regulação para Baixo/efeitos dos fármacos
Feminino
Seres Humanos
Células MCF-7
Metaloproteinase 2 da Matriz/metabolismo
Metaloproteinase 9 da Matriz/metabolismo
Fosforilação/efeitos dos fármacos
Inibidores de Proteínas Quinases/farmacologia
Proteínas Proto-Oncogênicas c-akt/metabolismo
Transdução de Sinais/efeitos dos fármacos
Inibidor Tecidual de Metaloproteinase-1/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (6-OH-11-O-hydroxyphenanthrene); 0 (Phenanthrenes); 0 (Protein Kinase Inhibitors); 0 (Tissue Inhibitor of Metalloproteinase-1); 136894-56-9 (Basigin); 8R1V1STN48 (Catechin); BQM438CTEL (epigallocatechin gallate); EC 2.7.10.1 (EGFR protein, human); EC 2.7.10.1 (Receptor, Epidermal Growth Factor); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt); EC 3.4.24.24 (Matrix Metalloproteinase 2); EC 3.4.24.35 (Matrix Metalloproteinase 9)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  5 / 8097 MEDLINE  
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[PMID]:29355013
[Au] Autor:Zhang W; Li X; Hua F; Chen W; Wang W; Chu GX; Bao GH
[Ad] Endereço:Natural Products Laboratory, International Joint Lab of Tea Chemistry and Health Effects, State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , Hefei, 230036 People's Republic of China.
[Ti] Título:Interaction between Ester-Type Tea Catechins and Neutrophil Gelatinase-Associated Lipocalin: Inhibitory Mechanism.
[So] Source:J Agric Food Chem;66(5):1147-1156, 2018 Feb 07.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tea is thought to alleviate neurotoxicity due to the antioxidative effect of ester-type tea catechins (ETC). Neutrophil gelatinase-associated lipocalin (NGAL) can sensitize ß-amyloid (Aß) induced neurotoxicity, and inhibitors of NGAL may relieve associated symptoms. As such, the interactions of ETC with NGAL were investigated by fluorescence spectrometry and molecular simulation. NGAL fluorescence is quenched regularly when being added with six processing types of tea infusion (SPTT) and ETC. Thermodynamic analyses suggest that ETC with more catechol moieties has a stronger binding capacity with NGAL especially in the presence of Fe . (-)-Epicatechin 3-O-caffeoate (ECC), a natural product isolated from Zijuan green tea, shows the strongest binding ability with NGAL (K = 15.21 ± 8.68 nM in the presence of Fe ). All ETC are effective in protecting nerve cells against H O or Aß induced injury. The inhibitory mechanism of ETC against NGAL supports its potential use in attenuation of neurotoxicity.
[Mh] Termos MeSH primário: Catequina/farmacologia
Lipocalina-2/farmacologia
Chá/química
[Mh] Termos MeSH secundário: Catequina/metabolismo
Interações Medicamentosas
Ésteres
Peróxido de Hidrogênio/farmacologia
Quelantes de Ferro
Lipocalina-2/antagonistas & inibidores
Lipocalina-2/metabolismo
Modelos Moleculares
Neurônios/efeitos dos fármacos
Fármacos Neuroprotetores
Espectrometria de Fluorescência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Esters); 0 (Iron Chelating Agents); 0 (Lipocalin-2); 0 (Neuroprotective Agents); 0 (Tea); 8R1V1STN48 (Catechin); BBX060AN9V (Hydrogen Peroxide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180123
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b05399


  6 / 8097 MEDLINE  
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[PMID]:29254289
[Au] Autor:Gerges Geagea A; Rizzo M; Eid A; Hajj Hussein I; Zgheib Z; Zeenny MN; Jurjus R; Uzzo ML; Spatola GF; Bonaventura G; Leone A; Massaad-Massade L; Jurjus A
[Ad] Endereço:Department of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut, Lebanon.
[Ti] Título:Tea catechins induce crosstalk between signaling pathways and stabilize mast cells in ulcerative colitis.
[So] Source:J Biol Regul Homeost Agents;31(4):865-877, 2017 Oct-Dec.
[Is] ISSN:0393-974X
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:It is well documented that nutraceuticals, in general, and Green tea catechins, in particular, possess a potential therapeutic value in inflammatory bowel diseases (IBD) due to their anti-oxidative and anti-inflammatory effects. This study aimed to investigate the possible mechanism of action of catechins in a rat model of colitis induced by 2.4.6 trinitrobenzene sulfonic acid (TNBS). Thirty-five young adult Sprague-Dawley rats were divided into four groups: normal control (n=5), catechins (n=9), TNBS (n=9) and TNBS plus catechins (n=12) treated. Catechin in the form of Epigallocatechin-3-gallate (EGCG) was administered daily by intraperitoneal injection, 1 week before the induction date of UC. Biopsies of the descending colon were collected on days 3, 10 and 17, and partly frozen for molecular studies or fixed for light microscopy. The status of intestinal tissue alterations and mast cells number were also assessed, as well as the mRNA expressions of IL-6, TNF-a and NF-kB, and determination of ROS expression. Histological data depicted a significant amelioration in the TNBS- and EGCG-treated rats compared to the non-treated animals. Catechin expressed strong anti-inflammatory and anti-oxidant effects, ameliorated ulcerative colitis and stabilized mast cells. The mechanism of action occurred basically through the NF-kB pathway and possibly through a crosstalk with other pathways.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Antioxidantes/farmacologia
Catequina/análogos & derivados
Colite/tratamento farmacológico
Colo/efeitos dos fármacos
Transdução de Sinais/efeitos dos fármacos
Chá/química
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/isolamento & purificação
Antioxidantes/isolamento & purificação
Catequina/isolamento & purificação
Catequina/farmacologia
Colite/induzido quimicamente
Colite/imunologia
Colite/patologia
Colo/imunologia
Colo/patologia
Regulação da Expressão Gênica
Interleucina-6/genética
Interleucina-6/imunologia
Masculino
Mastócitos/efeitos dos fármacos
Mastócitos/imunologia
Mastócitos/patologia
NF-kappa B/genética
NF-kappa B/imunologia
Ratos
Ratos Sprague-Dawley
Espécies Reativas de Oxigênio/imunologia
Espécies Reativas de Oxigênio/metabolismo
Ácido Trinitrobenzenossulfônico
Fator de Necrose Tumoral alfa/genética
Fator de Necrose Tumoral alfa/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antioxidants); 0 (Interleukin-6); 0 (NF-kappa B); 0 (Reactive Oxygen Species); 0 (Tea); 0 (Tumor Necrosis Factor-alpha); 8R1V1STN48 (Catechin); 8T3HQG2ZC4 (Trinitrobenzenesulfonic Acid); BQM438CTEL (epigallocatechin gallate)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE


  7 / 8097 MEDLINE  
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[PMID]:29223045
[Au] Autor:Aybastier Ö; Dawbaa S; Demir C
[Ad] Endereço:University of Uludag, Faculty of Science and Arts, Department of Chemistry, 16059, Bursa, Turkey.
[Ti] Título:Investigation of antioxidant ability of grape seeds extract to prevent oxidatively induced DNA damage by gas chromatography-tandem mass spectrometry.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1072:328-335, 2018 Jan 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Phenolic compounds have been studied elaborately for their efficacy to improve health and to protect against a wide variety of diseases. Herein this study, different analysis methods were implemented to evaluate the antioxidant properties of catechin and cyanidin using their standard substances and as they found in the grape seeds extracts. Total phenol contents were 107.39±8.94mg GAE/g dw of grape seeds for grape seed extract (GSE) and 218.32±10.66mg GAE/g dw of grape seeds for acid-hydrolyzed grape seed extract (AcGSE). The extracts were analyzed by HPLC-DAD system and the results showed the presence of catechin, gallic acid, chlorogenic acid and ellagic acid in the processed methanolic extract and cyanidin, gallic acid and ellagic acid in the processed acidified methanolic extract. The protective abilities of catechin and cyanidin were tested against the oxidation of DNA. The results showed that cyanidin has better protection of DNA against oxidation than catechin. GSE and AcGSE were revealed to inhibit the oxidatively induced DNA damage. GSE decreased about 57% of damage caused by the Fenton control sample. This study could show new aspects of the antioxidant profiles of cyanidin and catechin.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Dano ao DNA/efeitos dos fármacos
Cromatografia Gasosa-Espectrometria de Massas/métodos
Extratos Vegetais/farmacologia
Vitis/química
[Mh] Termos MeSH secundário: Animais
Antocianinas
Antioxidantes/química
Catequina
Bovinos
DNA/efeitos dos fármacos
Fenóis
Extratos Vegetais/química
Sementes/química
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Antioxidants); 0 (Phenols); 0 (Plant Extracts); 7732ZHU564 (cyanidin); 8R1V1STN48 (Catechin); 9007-49-2 (DNA); 91080-16-9 (calf thymus DNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171210
[St] Status:MEDLINE


  8 / 8097 MEDLINE  
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[PMID]:29227650
[Au] Autor:Wang X; Guo L; Gao L; Shi X; Zhao X; Ma X; Xia T; Wang Y
[Ad] Endereço:Life Science College and §State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , Hefei, Anhui 230036, People's Republic of China.
[Ti] Título:Molecular Evidence for Catechin Synthesis and Accumulation in Tea Buds (Camellia sinensis).
[So] Source:J Agric Food Chem;66(1):63-69, 2018 Jan 10.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Early spring buds of the Camellia sinensis variety Shuchazao were separated into two parts, including the shoot tip (ST) and non-expanded young leaves (YL), in which the synthesis and accumulation of catechins in the two parts were assessed by high-performance liquid chromatography (HPLC), p-dimethylaminocinnamaldehyde (DMACA) staining, quantitative real-time polymerase chain reaction (qRT-PCR), and in situ hybridization. HPLC showed that (-)-epigallocatechin-3-gallate (EGCG) and (-)-epicatechin-3-gallate (ECG) amounts in YL were increased significantly by 74.0 and 71.8%, respectively. The results of DMACA staining indicated that catechins in buds accumulated mainly in mesophyll cells and the bud shaft of YL. Meanwhile, qRT-PCR demonstrated that the relative expression levels of genes related to flavonoid metabolism, including CsPAL1, CsC4H1, CsC4H2, CsCHS2, CsF3'5'H1, CsDFR1, CsDFR2, and CsANR1, were significantly higher in YL than in the ST. In situ hybridization revealed that CsDFR1, CsDFR2, CsLAR, and CsANR1 were expressed in leaf primordia and YL but not in the apical meristem. These findings highlight the synthesis and accumulation patterns of catechins in different parts of the ST in C. sinensis, providing a theoretical basis for the assessment of synthesis, accumulation, and transfer patterns of catechins in tea plants.
[Mh] Termos MeSH primário: Camellia sinensis/metabolismo
Catequina/metabolismo
Folhas de Planta/metabolismo
[Mh] Termos MeSH secundário: Catequina/biossíntese
Catequina/genética
Cromatografia Líquida de Alta Pressão/métodos
Flavonoides/metabolismo
Regulação da Expressão Gênica de Plantas
Hibridização In Situ/métodos
Folhas de Planta/genética
Folhas de Planta/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flavonoids); 35HDD3NRIE (flavan-3-ol); 8R1V1STN48 (Catechin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b03205


  9 / 8097 MEDLINE  
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[PMID]:29313327
[Au] Autor:Chai WM; Huang Q; Lin MZ; Ou-Yang C; Huang WY; Wang YX; Xu KL; Feng HL
[Ad] Endereço:College of Life Science and Key Laboratory of Small Functional Organic Molecule, Ministry of Education, Jiangxi Normal University , Nanchang, Jiangxi 330022, People's Republic of China.
[Ti] Título:Condensed Tannins from Longan Bark as Inhibitor of Tyrosinase: Structure, Activity, and Mechanism.
[So] Source:J Agric Food Chem;66(4):908-917, 2018 Jan 31.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, the content, structure, antityrosinase activity, and mechanism of longan bark condensed tannins were evaluated. The findings obtained from mass spectrometry demonstrated that longan bark condensed tannins were mixtures of procyanidins, propelargonidins, prodelphinidins, and their acyl derivatives (galloyl and p-hydroxybenzoate). The enzyme analysis indicated that these mixtures were efficient, reversible, and mixed (competitive is dominant) inhibitor of tyrosinase. What's more, the mixtures showed good inhibitions on proliferation, intracellular enzyme activity and melanogenesis of mouse melanoma cells (B ). From molecular docking, the results showed the interactions between inhibitors and tyrosinase were driven by hydrogen bond, electrostatic, and hydrophobic interactions. In addition, high levels of total phenolic and extractable condensed tannins suggested that longan bark might be a good source of tyrosinase inhibitor. This study would offer theoretical basis for the development of longan bark condensed tannins as novel food preservatives and medicines of skin diseases.
[Mh] Termos MeSH primário: Inibidores Enzimáticos/farmacologia
Monofenol Mono-Oxigenase/antagonistas & inibidores
Casca de Planta/química
Sapindaceae/química
Taninos/química
Taninos/farmacologia
[Mh] Termos MeSH secundário: Animais
Antocianinas/farmacologia
Biflavonoides/farmacologia
Catequina/farmacologia
Proliferação Celular/efeitos dos fármacos
Ligações de Hidrogênio
Interações Hidrofóbicas e Hidrofílicas
Espectrometria de Massas
Melaninas/análise
Melaninas/antagonistas & inibidores
Melaninas/biossíntese
Melanoma Experimental
Camundongos
Modelos Moleculares
Simulação de Acoplamento Molecular
Oxirredutases
Parabenos/farmacologia
Proantocianidinas/farmacologia
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Eletricidade Estática
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Biflavonoids); 0 (Enzyme Inhibitors); 0 (Melanins); 0 (Parabens); 0 (Proanthocyanidins); 0 (Tannins); 4852-22-6 (procyanidin); 8R1V1STN48 (Catechin); EC 1.- (Oxidoreductases); EC 1.14.18.- (monophenolase); EC 1.14.18.1 (Monophenol Monooxygenase); EM6MD4AEHE (delphinidin); JG8Z55Y12H (4-hydroxybenzoic acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b05481


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[PMID]:27778132
[Au] Autor:Karthikeyan B; Harini L; Krishnakumar V; Kannan VR; Sundar K; Kathiresan T
[Ad] Endereço:Department of Biotechnology, Kalasalingam University, Anand Nagar, Krishnankoil, Tamil Nadu, 626 126, India.
[Ti] Título:Insights on the involvement of (-)-epigallocatechin gallate in ER stress-mediated apoptosis in age-related macular degeneration.
[So] Source:Apoptosis;22(1):72-85, 2017 Jan.
[Is] ISSN:1573-675X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Endoplasmic reticulum (ER) stress-mediated apoptosis is a well-known factor in the pathogenesis of age-related macular degeneration (AMD). ER stress leads to accumulation of misfolded proteins, which in turn activates unfolded protein response (UPR) of the cell for its survival. The prolonged UPR of ER stress promotes cell death; however, the transition between adaptation and ER stress-induced apoptosis has not been clearly understood. Hence, the present study investigates the regulatory effect of (-)-epigallocatechin gallate (EGCG) on ER stress-induced by hydrogen peroxide (H O ) and disturbance of calcium homeostasis by thapsigargin (TG) in mouse retinal pigment epithelial (MRPE) cells. The oxidant molecules influenced MRPE cells showed an increased level of intracellular calcium [Ca ] in ER and transferred to mitochondria through ER-mitochondrial tether site then increased ROS production. EGCG restores [Ca ] homeostasis by decreasing ROS production through inhibition of prohibitin1 which regulate ER-mitochondrial tether site and inhibit apoptosis. Effect of EGCG on ER stress-mediated apoptosis was elucidated by exploring the UPR signalling pathways. EGCG downregulated GRP78, CHOP, PERK, ERO1α, IRE1α, cleaved PARP, cleaved caspase 3, caspase 12 and upregulated expression of calnexinin MRPE cells. In addition to this, inhibition of apoptosis by EGCG was also confirmed with expression of proteins Akt, PTEN and GSK3ß. MRPE cells with EGCG upregulates phosphorylation of Akt at ser473 and phospho ser380 of PTEN, but phosphorylation at ser9 of GSK3ß was inhibited. Further, constitutively active (myristoylated) CA-Akt transfected in MRPE cells had an increased Akt activity in EGCG influenced cells. These findings strongly suggest that antioxidant molecules inhibit cell death through the proper balancing of [Ca ] and ROS production in order to maintain UPR of ER in MRPE cells. Thus, modulation of UPR signalling may provide a potential target for the therapeutic approaches of AMD.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Catequina/análogos & derivados
Estresse do Retículo Endoplasmático/efeitos dos fármacos
Degeneração Macular/tratamento farmacológico
Resposta a Proteínas não Dobradas/genética
[Mh] Termos MeSH secundário: Animais
Antioxidantes/metabolismo
Cálcio/metabolismo
Sinalização do Cálcio/genética
Catequina/administração & dosagem
Estresse do Retículo Endoplasmático/genética
Seres Humanos
Peróxido de Hidrogênio/toxicidade
Degeneração Macular/genética
Camundongos
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/genética
Estresse Oxidativo/efeitos dos fármacos
Espécies Reativas de Oxigênio/metabolismo
Tapsigargina/administração & dosagem
Resposta a Proteínas não Dobradas/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Reactive Oxygen Species); 67526-95-8 (Thapsigargin); 8R1V1STN48 (Catechin); BBX060AN9V (Hydrogen Peroxide); BQM438CTEL (epigallocatechin gallate); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE
[do] DOI:10.1007/s10495-016-1318-2



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