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[PMID]:29370193
[Au] Autor:Kalita D; Holm DG; LaBarbera DV; Petrash JM; Jayanty SS
[Ad] Endereço:San Luis Valley Research Center, Department of Horticulture and Landscape Architecture, Colorado State University, Center, United States of America.
[Ti] Título:Inhibition of α-glucosidase, α-amylase, and aldose reductase by potato polyphenolic compounds.
[So] Source:PLoS One;13(1):e0191025, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Diabetes mellitus is a chronic disease that is becoming a serious global health problem. Diabetes has been considered to be one of the major risks of cataract and retinopathy. Synthetic and natural product inhibitors of carbohydrate degrading enzymes are able to reduce type 2 diabetes and its complications. For a long time, potatoes have been portrayed as unhealthy for diabetic patients by some nutritionist due to their high starch content. However, purple and red potato cultivars have received considerable attention from consumers because they have high levels of polyphenolic compounds that have potent antioxidant activities. In this study, we screened the total phenolics (TP) and total anthocyanins (TA) and analyzed the phenolic and anthocyanin compounds in selected potato cultivars and advanced selections with distinct flesh colors (purple, red, yellow and white). Purple and red potato cultivars had higher levels of TP and TA than tubers with other flesh colors. Chlorogenic acid is the predominant phenolic acid, and major anthocyanin is composed of the derivatives of petunidin, peonidin, malvidin and pelargonidin. We tested the potential inhibitory effect of potato extracts on the activities of α-amylase and α-glucosidase, which were targeted to develop antidiabetic therapeutic agents. We also measured inhibitory effect of potato extracts on aldose reductase (AR) which is a key enzyme that has been a major drug target for the development of therapies to treat diabetic complications. Purple flesh tubers extract showed the most effective inhibition of α-amylase, α-glucosidase, and aldose reductase with IC50 values 25, 42, and 32 µg/ml, respectively. Kinetic studies showed that anthocyanins are noncompetitive inhibitors of these enzymes, whereas phenolic acids behaved as mixed inhibitors for α-amylase and α-glucosidase and noncompetitive inhibitors for AR. This study supports the development of a positive and healthful image of potatoes, which is an important issue for consumers.
[Mh] Termos MeSH primário: Aldeído Redutase/antagonistas & inibidores
Inibidores Enzimáticos/farmacologia
Polifenóis/farmacologia
Solanum tuberosum/química
alfa-Amilases/antagonistas & inibidores
alfa-Glucosidases/efeitos dos fármacos
[Mh] Termos MeSH secundário: Antocianinas/análise
Antocianinas/farmacologia
Cromatografia Líquida
Espectrometria de Massas
Polifenóis/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Enzyme Inhibitors); 0 (Polyphenols); EC 1.1.1.21 (Aldehyde Reductase); EC 3.2.1.1 (alpha-Amylases); EC 3.2.1.20 (alpha-Glucosidases)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191025


  2 / 5451 MEDLINE  
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[PMID]:28471391
[Au] Autor:Benito Á; Calderón F; Benito S
[Ad] Endereço:Departamento de Química y Tecnología de Alimentos, Universidad Politécnica de Madrid, Ciudad Universitaria S/N, 28040 Madrid, Spain. angel@urbinavinos.com.
[Ti] Título:The Combined Use of Schizosaccharomyces pombe and Lachancea thermotolerans-Effect on the Anthocyanin Wine Composition.
[So] Source:Molecules;22(5), 2017 May 04.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The most popular methodology to make red wine is through the combined use of yeast and lactic acid bacteria, for alcoholic fermentation and malolactic fermentation respectively. This classic winemaking practice produces stable red wines from a microbiological point of view. This study aims to investigate a recent red winemaking biotechnology, which through the combined use of and is used as an alternative to the classic malolactic fermentation. In this new methodology, totally consumes malic acid, while produces lactic acid, avoiding excessive deacidification of musts with low acidity in warm viticulture areas such as Spain. This new methodology has been reported to be a positive alternative to malolactic fermentation in low acidity wines, since it has the advantage to produce wines with a more fruity flavor, less acetic acid, less ethyl carbamate originators and less biogenic amines than the traditional wines produced via conventional fermentation techniques. The study focuses on unexplored facts related to this novel biotechnology such as color and anthocyanin profile.
[Mh] Termos MeSH primário: Antocianinas/análise
Saccharomycetales/metabolismo
Schizosaccharomyces/metabolismo
Vinho/análise
[Mh] Termos MeSH secundário: Fermentação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE


  3 / 5451 MEDLINE  
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[PMID]:28480755
[Au] Autor:He Y; Wen L; Liu J; Li Y; Zheng F; Min W; Yue H; Pan P
[Ad] Endereço:a Department of Food Science and Engineering , Jilin Agricultural University , Changchun , China.
[Ti] Título:Optimisation of pulsed electric fields extraction of anthocyanin from Beibinghong Vitis Amurensis Rupr.
[So] Source:Nat Prod Res;32(1):23-29, 2018 Jan.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Beibinghong Vitis amurensis Rupr has wide plantation area, high productivity and rich anthocyanin. Common hot-extraction has poor deficiency and destroys anthocyanin severely. For Beibinghong V. amurensis Rupr as materials, response surface-optimised electric fields were used, the structure of Beibinghong was observed by SEM, antioxidant activity was measured by DPPH, ABTS and reducing force, the component of anthocyanin was analyzed by HPLC-MS. We found the content of total anthocyanin extracted by pulsed electric fields was 166.65 ± 3.88 mg/100 g.FW. Total anthocyanin from Beibinghong had high antioxidant activity, also contained multiple steady anthocyanin of delphinidin 3-O-glucoside, cyanidin 3-O-glucoside, petunidin 3-O-glucoside, peonidin 3-O-glucoside, malvidin 3-O-glucoside, delphinidin-3-O-(6-O-acetyl) glucoside and delphinidin-3-O-(6-O-p-coumaroyl) glucoside et al. In conclusion, the optimised pulsed electric fields method can quickly and efficiently extract several kinds of anthocyanins from V. amurensis Rupr. This study promoted the intensive processing of V. amurensis Rupr and widened the practical application of pulsed electric field technology.
[Mh] Termos MeSH primário: Antocianinas/isolamento & purificação
Fracionamento Químico/métodos
Vitis/química
[Mh] Termos MeSH secundário: Antocianinas/análise
Antocianinas/farmacologia
Antioxidantes/isolamento & purificação
Antioxidantes/farmacologia
Cromatografia Líquida de Alta Pressão/métodos
Estimulação Elétrica
Glucosídeos/análise
Espectrometria de Massas/métodos
Microscopia Eletrônica de Varredura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Antioxidants); 0 (Glucosides); 0 (delphinidin 3-O-glucopyranoside); 3419IIT8I0 (peonidin 3-glucoside); 6988-81-4 (petunidin-3-glucoside); 7084-24-4 (cyanidin 3-O-glucoside); 7228-78-6 (malvidin-3-glucoside)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170509
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2017.1324963


  4 / 5451 MEDLINE  
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[PMID]:28658976
[Au] Autor:Haddar W; Ben Ticha M; Meksi N; Guesmi A
[Ad] Endereço:a Faculté des Sciences , Université de Monastir, Unité Chimie Appliquée - Environnement UR13ES63 , Monastir , Tunisia.
[Ti] Título:Application of anthocyanins as natural dye extracted from Brassica oleracea L. var. capitata f. rubra: dyeing studies of wool and silk  fibres.
[So] Source:Nat Prod Res;32(2):141-148, 2018 Jan.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Brassica oleracea L. var. capitata f. rubra (Red Cabbage) dye is composed mainly of natural pigment called anthocyanins used as a natural colourant. Wool and silk fibres were dyed with the aqueous extract obtained from red cabbage. The dyeing process was investigated and the combined effects of dyeing conditions on the colour yield parameter (K/S) were studied. Resulted fastness to wash, rubbing and light of the dyed fabrics were evaluated. Chemical Oxygen Demand (COD) and Biological Oxygen Demand (BOD of the residual effluent were measured. Best dyeing conditions were found to be: 50 g/100 mL, pH 2, 60 min and 100 °C, respectively, for the red cabbage weight, pH, dyeing duration and temperature. Good fastnesses properties were found in both cases: for wool and silk fabrics. It was found also that the calculated biodegradability ratio (COD/BOD ) of the residual bath of dyeing wool and silk with red cabbage extract are lower than 1.5 which means that these baths are biodegradable.
[Mh] Termos MeSH primário: Antocianinas/química
Brassica/química
Corantes/isolamento & purificação
Seda

[Mh] Termos MeSH secundário: Animais
Antocianinas/isolamento & purificação
Biodegradação Ambiental
Análise da Demanda Biológica de Oxigênio
Corantes/química
Temperatura Ambiente
Têxteis
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Coloring Agents); 0 (Silk)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170630
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2017.1342080


  5 / 5451 MEDLINE  
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[PMID]:29360842
[Au] Autor:Liu F; Yang Y; Gao J; Ma C; Bi Y
[Ad] Endereço:College of Life Science, Shandong Normal University, Jinan, China.
[Ti] Título:A comparative transcriptome analysis of a wild purple potato and its red mutant provides insight into the mechanism of anthocyanin transformation.
[So] Source:PLoS One;13(1):e0191406, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, a red mutant was obtained through in vitro regeneration of a wild purple potato. High-performance liquid chromatography and Mass spectrometry analysis revealed that pelargonidin-3-O-glucoside and petunidin-3-O-glucoside were main anthocyanins in the mutant and wild type tubers, respectively. In order to thoroughly understand the mechanism of anthocyanin transformation in two materials, a comparative transcriptome analysis of the mutant and wild type was carried out through high-throughput RNA sequencing, and 295 differentially expressed genes (DEGs) were obtained. Real-time qRT-PCR validation of DEGs was consistent with the transcriptome date. The DEGs mainly influenced biological and metabolic pathways, including phenylpropanoid biosynthesis and translation, and biosynthesis of flavone and flavonol. In anthocyanin biosynthetic pathway, the analysis of structural genes expressions showed that three genes, one encoding phenylalanine ammonia-lyase, one encoding 4-coumarate-CoA ligase and one encoding flavonoid 3',5'-hydroxylasem were significantly down-regulated in the mutant; one gene encoding phenylalanine ammonia-lyase was significantly up-regulated. Moreover, the transcription factors, such as bZIP family, MYB family, LOB family, MADS family, zf-HD family and C2H2 family, were significantly regulated in anthocyanin transformation. Response proteins of hormone, such as gibberellin, abscisic acid and brassinosteroid, were also significantly regulated in anthocyanin transformation. The information contributes to discovering the candidate genes in anthocyanin transformation, which can serve as a comprehensive resource for molecular mechanism research of anthocyanin transformation in potatoes.
[Mh] Termos MeSH primário: Antocianinas/biossíntese
Antocianinas/genética
Solanum tuberosum/genética
Solanum tuberosum/metabolismo
[Mh] Termos MeSH secundário: Vias Biossintéticas/genética
Coenzima A Ligases/genética
Sistema Enzimático do Citocromo P-450/genética
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Genes de Plantas
Glucosídeos/biossíntese
Glucosídeos/genética
Sequenciamento de Nucleotídeos em Larga Escala
Mutação
Fenilalanina Amônia-Liase/genética
Pigmentação/genética
Reguladores de Crescimento de Planta/genética
Proteínas de Plantas/genética
Tubérculos/genética
Tubérculos/metabolismo
RNA de Plantas/genética
Fatores de Transcrição/genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Glucosides); 0 (Plant Growth Regulators); 0 (Plant Proteins); 0 (RNA, Plant); 0 (Transcription Factors); 6988-81-4 (petunidin-3-glucoside); 8H1WZY9R6P (pelargonidin-3-glucoside); 9035-51-2 (Cytochrome P-450 Enzyme System); EC 1.14.- (flavonoid 3',5'-hydroxylase); EC 4.3.1.24 (Phenylalanine Ammonia-Lyase); EC 6.2.1.- (Coenzyme A Ligases); EC 6.2.1.12 (4-coumarate-CoA ligase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180124
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191406


  6 / 5451 MEDLINE  
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[PMID]:29333856
[Au] Autor:Xie Y; Zhu X; Li Y; Wang C
[Ad] Endereço:School of Food Science and Technology, Henan University of Technology , Zhengzhou, Henan 450001, People's Republic of China.
[Ti] Título:Analysis of the pH-Dependent Fe(III) Ion Chelating Activity of Anthocyanin Extracted from Black Soybean [Glycine max (L.) Merr.] Coats.
[So] Source:J Agric Food Chem;66(5):1131-1139, 2018 Feb 07.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Fe(III) chelating activity of anthocyanin extracted from black soybean coats was investigated at pH 3.0, 5.0, 6.5, 7.0, and 7.4 with fluorescence spectroscopy and microscale thermophoresis (MST). Cyanidin-3-glucoside (C3G) was determined to be 98% of the total anthocyanin by high-performance liquid chromatography. The binding affinity (K ) exhibited significant pH-dependent behavior: K was 9.7167 × 10 , 1.0837 × 10 , 1.4284 × 10 , 5.4550 × 10 , and 3.0269 × 10 M at pH 3.0, 5.0, 6.5, 7.0, and 7.4, respectively (p < 0.05). The MST data showed that ΔG < 0 and ΔH < 0, demonstrating that chelation is spontaneous and exothermic. Because both ΔH and ΔS < 0, the chelation involves hydrogen bonds and/or van der Waals forces for pH 3.0, 5.0, and 6.5. Electrostatic interactions contributed to chelation at pH 7.0 and 7.4 with ΔH < 0 and ΔS > 0. With the formation of chelates, C3G improved the solubility of Fe(III) at pH 6.5, 7.0, and 7.4 to enhance the ferric ion bioavailability, except for aggregation observed at pH 5.0.
[Mh] Termos MeSH primário: Antocianinas/química
Antocianinas/isolamento & purificação
Compostos Férricos/química
Quelantes de Ferro/química
Feijão de Soja/química
[Mh] Termos MeSH secundário: Disponibilidade Biológica
Ligações de Hidrogênio
Concentração de Íons de Hidrogênio
Solubilidade
Espectrometria de Fluorescência
Eletricidade Estática
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Ferric Compounds); 0 (Iron Chelating Agents)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180116
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04719


  7 / 5451 MEDLINE  
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[PMID]:29309888
[Au] Autor:Li Y; Fang J; Qi X; Lin M; Zhong Y; Sun L
[Ad] Endereço:Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, PR China.
[Ti] Título:A key structural gene, AaLDOX, is involved in anthocyanin biosynthesis in all red-fleshed kiwifruit (Actinidia arguta) based on transcriptome analysis.
[So] Source:Gene;648:31-41, 2018 Mar 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Study on kiwifruit (Actinidia chinensis and A. deliciosa) color mainly concentrated in green and yellow-fleshed cultivars, less about molecular mechanism of red-fleshed trait formation, rarely in all red-fleshed fruit. Using 'Tianyuanhong' and 'Yongfengyihao' ('TY', a kind of all red-fleshed cultivar, from Actinidia arguta; 'YF', a kind of all green-fleshed cultivar, also from Actinidia arguta) as experimental material, we performed RNA-seq to obtain 202,742 unigenes with an average length of 603bp and N50 of 873bp via transcriptome data analysis. Of these unigenes, 72,508 (35.76%) were annotated and 997 were assigned to secondary metabolic pathways, of which 104 unigenes were involved in flavonoid and anthocyanin biosynthesis. According to the parameter log2fold-change and p-adjusted, 12 differentially expressed structural genes were selected for performing expression profiles and cluster analysis. Physiological traits including color ration, hue angle, and anthocyanin content were also investigated. From the results, we concluded AaLDOX (genes encoding leucoanthocyanidin dioxygenase) maybe the key gene controlling anthocyanin biosynthesis in flesh of 'TY' kiwifruit, which promoted accumulation of anthocyanin, finally leading to the red flesh coloration.
[Mh] Termos MeSH primário: Actinidia/genética
Antocianinas/biossíntese
Frutas/genética
Perfilação da Expressão Gênica/métodos
Oxigenases/genética
Proteínas de Plantas/genética
[Mh] Termos MeSH secundário: Actinidia/metabolismo
Vias Biossintéticas/genética
Frutas/metabolismo
Regulação Enzimológica da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Ontologia Genética
Oxigenases/metabolismo
Proteínas de Plantas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Plant Proteins); EC 1.13.- (Oxygenases); EC 1.14.11.- (leucoanthocyanidin dioxygenase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180109
[St] Status:MEDLINE


  8 / 5451 MEDLINE  
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[PMID]:29037704
[Au] Autor:Mazewski C; Liang K; Gonzalez de Mejia E
[Ad] Endereço:Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, USA. Electronic address: cmazews2@illlinois.edu.
[Ti] Título:Comparison of the effect of chemical composition of anthocyanin-rich plant extracts on colon cancer cell proliferation and their potential mechanism of action using in vitro, in silico, and biochemical assays.
[So] Source:Food Chem;242:378-388, 2018 Mar 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The objective was to compare the anti-proliferative effect of anthocyanin-rich plant extracts on human colon cancer cells and determine their mechanism of action. Eleven extracts were tested: red (RG) and purple grape, purple sweet potato, purple carrot, black and purple bean, black lentil (BL), black peanut, sorghum (SH), black rice, and blue wheat. HCT-116 and HT-29 inhibition correlated with total phenolics (r=0.87 and 0.77, respectively), delphinidin-3-O-glucoside concentration with HT-29 inhibition (r=0.69). The concentration inhibition fifty (IC ) for BL, SH, RG on HT-29 and HCT-116 cell proliferation ranged 0.9-2.0mg/mL. Extracts decreased expression of anti-apoptotic proteins (survivin, cIAP-2, XIAP), induced apoptosis, and arrested cells in G1. Anthocyanins exhibited tyrosine kinase inhibitory potential in silico and biochemically; cyanidin-3-O-glucoside had one of the highest binding affinities with all kinases, especially ABL1 (-8.5kcal/mol). Cyanidin-3-O-glucoside and delphinidin-3-O-glucoside inhibited EGFR (IC =0.10 and 2.37µM, respectively). Cyanidin-3-O-glucoside was the most potent anthocyanin on kinase inhibition.
[Mh] Termos MeSH primário: Antocianinas/farmacologia
Proliferação Celular/efeitos dos fármacos
Neoplasias do Colo/tratamento farmacológico
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Neoplasias do Colo/fisiopatologia
Glucosídeos/farmacologia
Células HCT116
Células HT29
Seres Humanos
Fenóis/farmacologia
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Glucosides); 0 (Phenols); 0 (Plant Extracts); 0 (delphinidin 3-O-glucopyranoside); 7084-24-4 (cyanidin 3-O-glucoside)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171018
[St] Status:MEDLINE


  9 / 5451 MEDLINE  
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[PMID]:29223045
[Au] Autor:Aybastier Ö; Dawbaa S; Demir C
[Ad] Endereço:University of Uludag, Faculty of Science and Arts, Department of Chemistry, 16059, Bursa, Turkey.
[Ti] Título:Investigation of antioxidant ability of grape seeds extract to prevent oxidatively induced DNA damage by gas chromatography-tandem mass spectrometry.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1072:328-335, 2018 Jan 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Phenolic compounds have been studied elaborately for their efficacy to improve health and to protect against a wide variety of diseases. Herein this study, different analysis methods were implemented to evaluate the antioxidant properties of catechin and cyanidin using their standard substances and as they found in the grape seeds extracts. Total phenol contents were 107.39±8.94mg GAE/g dw of grape seeds for grape seed extract (GSE) and 218.32±10.66mg GAE/g dw of grape seeds for acid-hydrolyzed grape seed extract (AcGSE). The extracts were analyzed by HPLC-DAD system and the results showed the presence of catechin, gallic acid, chlorogenic acid and ellagic acid in the processed methanolic extract and cyanidin, gallic acid and ellagic acid in the processed acidified methanolic extract. The protective abilities of catechin and cyanidin were tested against the oxidation of DNA. The results showed that cyanidin has better protection of DNA against oxidation than catechin. GSE and AcGSE were revealed to inhibit the oxidatively induced DNA damage. GSE decreased about 57% of damage caused by the Fenton control sample. This study could show new aspects of the antioxidant profiles of cyanidin and catechin.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Dano ao DNA/efeitos dos fármacos
Cromatografia Gasosa-Espectrometria de Massas/métodos
Extratos Vegetais/farmacologia
Vitis/química
[Mh] Termos MeSH secundário: Animais
Antocianinas
Antioxidantes/química
Catequina
Bovinos
DNA/efeitos dos fármacos
Fenóis
Extratos Vegetais/química
Sementes/química
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Antioxidants); 0 (Phenols); 0 (Plant Extracts); 7732ZHU564 (cyanidin); 8R1V1STN48 (Catechin); 9007-49-2 (DNA); 91080-16-9 (calf thymus DNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171210
[St] Status:MEDLINE


  10 / 5451 MEDLINE  
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[PMID]:29217480
[Au] Autor:Aichinger G; Puntscher H; Beisl J; Kütt ML; Warth B; Marko D
[Ad] Endereço:Department of Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, Waehringerstr. 38, A-1090 Vienna, Austria.
[Ti] Título:Delphinidin protects colon carcinoma cells against the genotoxic effects of the mycotoxin altertoxin II.
[So] Source:Toxicol Lett;284:136-142, 2018 Mar 01.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Alternaria spp. are ubiquitous molds that are able to produce toxic secondary metabolites which may contaminate food globally. One of those is the mycotoxin altertoxin II (ATX-II), a genotoxic and mutagenic compound. In recent years, different flavonoids that may co-occur with mycotoxins in food were demonstrated to temper toxic effects of molds, mostly through their anti-oxidant properties. Thus, in this study, we assessed the influence of the berry anthocyanidin delphinidin on the toxicity of ATX-II in HT-29 colon carcinoma cells. We performed coupled SRB/WST-1 cytotoxicity assays which revealed only weak antagonistic interactions, and single-cell gel electrophoresis ("comet") assays, where we observed a potent protective effect of delphinidin on the DNA-damaging properties of ATX-II. Furthermore, we investigated the mechanism for this interaction. In the DCF assay delphinidin was found to reduce intracellular oxidative stress levels, which might contribute partly to the latter protection. However, LC-MS experiments showed that co-incubation of the mycotoxin with either delphinidin or its potential degradation product phloroglucinol aldehyde significantly decreased ATX-II concentrations in aqueous solutions, indicating that a direct chemical reaction of ATX-II with these components is likely responsible for the observed loss of toxicity. Our results indicate that delphinidin - and possibly other anthocyanins as well - might play a role in the protection of the gut from Alternaria-induced genotoxicity.
[Mh] Termos MeSH primário: Antocianinas/farmacologia
Antioxidantes/farmacologia
Benzo(a)Antracenos/toxicidade
Dano ao DNA/efeitos dos fármacos
Mutagênicos/toxicidade
[Mh] Termos MeSH secundário: Alternaria/crescimento & desenvolvimento
Alternaria/metabolismo
Benzo(a)Antracenos/isolamento & purificação
Contagem de Células
Técnicas de Cultura de Células
Sobrevivência Celular/efeitos dos fármacos
Ensaio Cometa
Relação Dose-Resposta a Droga
Microbiologia de Alimentos
Células HT29
Seres Humanos
Estrutura Molecular
Mutagênicos/isolamento & purificação
Estresse Oxidativo/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Antioxidants); 0 (Benz(a)Anthracenes); 0 (Mutagens); 56257-59-1 (altertoxin II); EM6MD4AEHE (delphinidin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE



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