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[PMID]:28460467
[Au] Autor:Chen P; Zhang JY; Sha BB; Ma YE; Hu T; Ma YC; Sun H; Shi JX; Dong ZM; Li P
[Ad] Endereço:Cancer Chemoprevention Collaborative Innovation Center in Henan Province, Zhengzhou University, Zhengzhou, Henan, 450001, China.
[Ti] Título:Luteolin inhibits cell proliferation and induces cell apoptosis via down-regulation of mitochondrial membrane potential in esophageal carcinoma cells EC1 and KYSE450.
[So] Source:Oncotarget;8(16):27471-27480, 2017 Apr 18.
[Is] ISSN:1949-2553
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In current study, we investigated the anti-tumor effect of luteolin in human ESCC cell lines in vitro and in vivo and tried to explore the potential mechanisms. Results from flow cytometry showed that luteolin could induce apoptosis and caspase-3 activation and induce cell cycle arrest at G2/M phase in a dose- and time-dependent manner in EC1 and KYSE450 cells. JC-1 test results showed that membrane potential of mitochondria after luteolin treatment was down-regulated and this was an indicator for intrinsic apoptosis. Western Blot results showed the expression of cell cycle regulatory protein p21 and p53 increased and three apoptosis related proteins that participate in mitochondrial apoptotic pathway, namely, Bim, CYT-c and cPARP, also increased in luteolin treated cells compared with control groups. We further confirmed that luteolin could significantly inhibit the growth of ESCC tumors in xenograft mouse models and no evidence of systemic toxicity was observed. Our results suggest that luteolin can induce cell apoptosis and cell cycle arrest in G2/M phase through mitochondrial pathway in EC1 and KYSE450 cell lines and proper utilization of luteolin might be a practical approach in ESCC chemotherapy.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Luteolina/farmacologia
Potencial da Membrana Mitocondrial/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Proteína 11 Semelhante a Bcl-2/genética
Proteína 11 Semelhante a Bcl-2/metabolismo
Caspase 3/metabolismo
Ciclo Celular/efeitos dos fármacos
Pontos de Checagem do Ciclo Celular/efeitos dos fármacos
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Inibidor de Quinase Dependente de Ciclina p21/genética
Inibidor de Quinase Dependente de Ciclina p21/metabolismo
Modelos Animais de Doenças
Neoplasias Esofágicas
Feminino
Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos
Seres Humanos
Camundongos
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/metabolismo
Poli(ADP-Ribose) Polimerases/genética
Poli(ADP-Ribose) Polimerases/metabolismo
Proteína Supressora de Tumor p53/genética
Proteína Supressora de Tumor p53/metabolismo
Ensaios Antitumorais Modelo de Xenoenxerto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bcl-2-Like Protein 11); 0 (Cyclin-Dependent Kinase Inhibitor p21); 0 (Tumor Suppressor Protein p53); EC 2.4.2.30 (Poly(ADP-ribose) Polymerases); EC 3.4.22.- (Caspase 3); KUX1ZNC9J2 (Luteolin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.18632/oncotarget.15832


  2 / 1296 MEDLINE  
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[PMID]:28449600
[Au] Autor:Adaszynska-Skwirzynska M; Dzieciol M
[Ad] Endereço:a Faculty of Biotechnology and Animal Husbandry , West Pomeranian University of Technology , Szczecin , Poland.
[Ti] Título:Comparison of phenolic acids and flavonoids contents in various cultivars and parts of common lavender (Lavandula angustifolia) derived from Poland.
[So] Source:Nat Prod Res;31(21):2575-2580, 2017 Nov.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of study was to compare the content of phenolic acids and flavonoids in two cultivars of Lavandula angustifolia: 'Blue River' and 'Ellagance Purple', including flowers and leafy stalks. Total phenolics and total flavonoids contents were determined by UV-Vis spectroscopy. The contents of total phenolics in leafy stalks (3.71-4.06 mg g d.m.) were higher than in flowers (1.13-1.14 mg g d.m.). Similarly, higher total contents of flavonoids were determined in leafy stalks (3.41-3.51 mg g d.m.), as compared with flowers (0.86-0.91 mg g d.m.). Phenolic acids and flavonoids were identified and quantified using HPLC and UPLC methods. Three phenolic acids were determined: rosmarinic, ferulic and caffeic acid. Lavender extracts contained also flavonoids from group of apigenin, luteolin and quercetin. Higher amounts of luteolin diglucuronide and luteolin glucuronide were found in leafy stalks in comparison to flowers. Obtained results indicate that leafy stalks of lavender can be also valuable source of antioxidant compounds.
[Mh] Termos MeSH primário: Flavonoides/análise
Hidroxibenzoatos/análise
Lavandula/química
[Mh] Termos MeSH secundário: Apigenina/análise
Ácidos Cafeicos/análise
Cromatografia Líquida de Alta Pressão/métodos
Flavonoides/química
Flores/química
Hidroxibenzoatos/química
Luteolina/análise
Óleos Voláteis/análise
Fenóis/análise
Polônia
Quercetina/análise
Espectrofotometria Ultravioleta/métodos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Caffeic Acids); 0 (Flavonoids); 0 (Hydroxybenzoates); 0 (Oils, Volatile); 0 (Phenols); 29656-58-4 (phenolic acid); 7V515PI7F6 (Apigenin); 9IKM0I5T1E (Quercetin); KUX1ZNC9J2 (Luteolin); U2S3A33KVM (caffeic acid)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2017.1320792


  3 / 1296 MEDLINE  
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[PMID]:28681916
[Au] Autor:Song F; Wei C; Zhou L; Qin A; Yang M; Tickner J; Huang Y; Zhao J; Xu J
[Ad] Endereço:Research Centre for Regenerative Medicine, Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning, Guangxi, China.
[Ti] Título:Luteoloside prevents lipopolysaccharide-induced osteolysis and suppresses RANKL-induced osteoclastogenesis through attenuating RANKL signaling cascades.
[So] Source:J Cell Physiol;233(2):1723-1735, 2018 Feb.
[Is] ISSN:1097-4652
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bone destruction or osteolysis marked by excessive osteoclastic bone resorption is a very common medical condition. Identification of agents that can effectively suppress excessive osteoclast formation and function is crucial for prevention and treatment of osteolytic conditions such as periprosthetic joint infection and periprosthetic loosening. Luteoloside, a flavonoid, is a natural bioactive compound with anti-inflammation and anti-tumor properties. However, the effect of Luteoloside on inflammation-induced osteolysis is unknown. Here, we found that Luteoloside exhibited a strong inhibitory effect on lipopolysaccharide (LPS)-induced osteolysis in vivo. In addition, Luteoloside suppressed RANKL-induced osteoclast differentiation and abrogated bone resorption in a dose-dependent manner. Further, we found that the anti-osteoclastic and anti-resorptive actions of Luteoloside are mediated via blocking NFATc1 activity and the attenuation of RANKL-mediated Ca signaling as well as NF-κB and MAPK pathways. Taken together, this study shows that Luteoloside may be a potential therapeutic agent for osteolytic bone diseases associated with abnormal osteoclast formation and function in inflammatory conditions.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Glucosídeos/farmacologia
Lipopolissacarídeos
Luteolina/farmacologia
Osteoclastos/efeitos dos fármacos
Osteogênese/efeitos dos fármacos
Osteólise/prevenção & controle
Ligante RANK/metabolismo
Crânio/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Sinalização do Cálcio/efeitos dos fármacos
Modelos Animais de Doenças
Relação Dose-Resposta a Droga
Durapatita/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Proteínas Quinases Ativadas por Mitógeno/metabolismo
NF-kappa B/metabolismo
Fatores de Transcrição NFATC/metabolismo
Osteoclastos/metabolismo
Osteoclastos/patologia
Osteólise/induzido quimicamente
Osteólise/metabolismo
Osteólise/patologia
Células RAW 264.7
Crânio/metabolismo
Crânio/patologia
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Glucosides); 0 (Lipopolysaccharides); 0 (NF-kappa B); 0 (NFATC Transcription Factors); 0 (Nfatc1 protein, mouse); 0 (RANK Ligand); 0 (Tnfsf11 protein, mouse); 91D9GV0Z28 (Durapatite); 98J6XDS46I (luteolin-7-glucoside); EC 2.7.11.24 (Mitogen-Activated Protein Kinases); KUX1ZNC9J2 (Luteolin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170707
[St] Status:MEDLINE
[do] DOI:10.1002/jcp.26084


  4 / 1296 MEDLINE  
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[PMID]:28878808
[Au] Autor:Gutiérrez-Venegas G; Torras-Ceballos A; Gómez-Mora JA; Fernández-Rojas B
[Ad] Endereço:Laboratorio de Bioquímica de la División de Estudios de Posgrado de la Facultad de Odontología, Universidad Nacional Autónoma de México Ciudad Universitaria, 04510 México DF, Mexico.
[Ti] Título:Luteolin, quercetin, genistein and quercetagetin inhibit the effects of lipopolysaccharide obtained from in H9c2 cardiomyoblasts.
[So] Source:Cell Mol Biol Lett;22:19, 2017.
[Is] ISSN:1689-1392
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: One of the microorganisms from dental plaque associated with severe inflammatory responses in infectious endocarditis is . It is a Gram-negative bacteria harvested from chronic periodontitis patients. Lipopolysaccharide (LPS) obtained from promotes the expressions of interleukin-1 (IL-1), IL-6 and tumor necrosis factor alpha (TNF-α). Flavonoids are thought to participate in processes that control inflammation, such as the expression of cyclooxygenase-2 (COX-2). METHODS: We investigated the effects of luteolin, quercetin, genistein and quercetagetin on cardiomyoblasts treated with LPS alone or in combination with following inhibitors p38 (SB203580), ERK (PD98059), JNK (SP600125) and PKC (Calphostin C) for 1 h. The kinase activation and COX-2 expression levels were determined at the gene and protein levels. RESULTS: These flavonoids are considered to inhibit the activation of mitogen-activated protein kinase (MAPK) and the degradation of inhibitor of kappa B-alpha (IκB-α). They also play a role in COX-2 expression. CONCLUSION: We conclude that the tested flavonoids inhibit inflammatory responses induced by LPS in H9c2 cells.
[Mh] Termos MeSH primário: Cromonas/farmacologia
Ciclo-Oxigenase 2/efeitos dos fármacos
Flavonoides/farmacologia
Lipopolissacarídeos/toxicidade
Proteínas Quinases Ativadas por Mitógeno/efeitos dos fármacos
Miócitos Cardíacos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Ciclo-Oxigenase 2/genética
Regulação da Expressão Gênica
Genisteína/farmacologia
Inflamação
Luteolina/farmacologia
Proteínas Quinases Ativadas por Mitógeno/antagonistas & inibidores
Miócitos Cardíacos/enzimologia
Miócitos Cardíacos/patologia
Porphyromonas gingivalis/química
Quercetina/farmacologia
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromones); 0 (Flavonoids); 0 (Lipopolysaccharides); 9IKM0I5T1E (Quercetin); DH2M523P0H (Genistein); EC 1.14.99.1 (Cyclooxygenase 2); EC 2.7.11.24 (Mitogen-Activated Protein Kinases); KUX1ZNC9J2 (Luteolin); SV68G507VO (quercetagetin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170908
[St] Status:MEDLINE
[do] DOI:10.1186/s11658-017-0047-z


  5 / 1296 MEDLINE  
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[PMID]:28825481
[Au] Autor:Mary V; Haris P; Varghese MK; Aparna P; Sudarsanakumar C
[Ad] Endereço:School of Pure and Applied Physics, Mahatma Gandhi University , Kottayam, Kerala 686560, India.
[Ti] Título:Experimental Probing and Molecular Dynamics Simulation of the Molecular Recognition of DNA Duplexes by the Flavonoid Luteolin.
[So] Source:J Chem Inf Model;57(9):2237-2249, 2017 Sep 25.
[Is] ISSN:1549-960X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Luteolin (C H O ) is an important flavonoid found in many fruits, plants, medicinal herbs, and vegetables exhibiting many pharmacological properties. The anticancer, antitumor, antioxidant, and anti-inflammatory activities of luteolin have been reported. The pharmacological action of small molecules is dependent upon its interaction with biomacromolecules. The interactions of small molecules with DNA play a major role in the transcription and translation process. In this work, we explored the energetic profile of DNA-luteolin interaction by isothermal titration calorimetry (ITC). The effect of temperature and salt concentration on DNA binding was examined by UV-Vis method. The mode of interaction was further probed by UV melting temperature analysis and differential scanning calorimetry. An atomic level insight on the recognition of luteolin with DNA was achieved by employing molecular dynamics (MD) simulation on luteolin in complex with AT- and GC-rich DNA sequences. AMBER force field proves to be appropriate in providing an understanding on the binding mode and specificity of luteolin with duplex DNA. MD results suggest a minor groove binding of luteolin with DNA and the binding free energy obtained is in agreement with the experimental results.
[Mh] Termos MeSH primário: DNA/metabolismo
Luteolina/metabolismo
Simulação de Dinâmica Molecular
[Mh] Termos MeSH secundário: Animais
Bovinos
DNA/química
Ligações de Hidrogênio
Conformação de Ácido Nucleico
Desnaturação de Ácido Nucleico
Termodinâmica
Temperatura de Transição
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-49-2 (DNA); KUX1ZNC9J2 (Luteolin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170822
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jcim.6b00747


  6 / 1296 MEDLINE  
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[PMID]:28655612
[Au] Autor:Zang M; Hu L; Zhang B; Zhu Z; Li J; Zhu Z; Yan M; Liu B
[Ad] Endereço:Department of General Surgery, Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, People's Republic of China.
[Ti] Título:Luteolin suppresses angiogenesis and vasculogenic mimicry formation through inhibiting Notch1-VEGF signaling in gastric cancer.
[So] Source:Biochem Biophys Res Commun;490(3):913-919, 2017 Aug 26.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gastric cancer is a great threat to the health of the people worldwide and lacks effective therapeutic regimens. Luteolin is one of Chinese herbs and presents in many fruits and green plants. In our previous study, we observed that luteolin inhibited cell migration and promoted cell apoptosis in gastric cancer. In the present study, luteolin significantly inhibited tube formation of human umbilical vein endothelial cells (HUVECs) through decreasing cell migration and proliferation of HUVECs in a dose-dependent manner. Vasculogenic mimicry (VM) tubes formed by gastric cancer cells were also inhibited with luteolin treatment. To explore how luteolin inhibited tubes formation, ELISA assay for VEGF was performed. Both of the VEGF secretion from Hs-746T cells and HUVECs were significantly decreased subsequent to luteolin treatment. In addition, cell migration was increased with the interaction between gastric cancer cells and HUVECs in co-culture assays. However, the promoting effects were abolished subsequent to luteolin treatment. Furthermore, luteolin inhibited VEGF secretion through suppressing Notch1 expression in gastric cancer. Overexpression of Notch1 in gastric cancer cells partially rescued the effects on cell migration, proliferation, HUVECs tube formation, and VM formation induced by luteolin treatment. In conclusion, luteolin inhibits angiogenesis and VM formation in gastric cancer through suppressing VEGF secretion dependent on Notch1 expression.
[Mh] Termos MeSH primário: Inibidores da Angiogênese/farmacologia
Luteolina/farmacologia
Neovascularização Patológica/tratamento farmacológico
Receptor Notch1/metabolismo
Neoplasias Gástricas/irrigação sanguínea
Neoplasias Gástricas/tratamento farmacológico
Fator A de Crescimento do Endotélio Vascular/metabolismo
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Células Endoteliais da Veia Umbilical Humana
Seres Humanos
Neovascularização Patológica/metabolismo
Neovascularização Patológica/patologia
Neovascularização Fisiológica/efeitos dos fármacos
Transdução de Sinais/efeitos dos fármacos
Estômago/irrigação sanguínea
Estômago/efeitos dos fármacos
Estômago/patologia
Neoplasias Gástricas/metabolismo
Neoplasias Gástricas/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Angiogenesis Inhibitors); 0 (NOTCH1 protein, human); 0 (Receptor, Notch1); 0 (Vascular Endothelial Growth Factor A); KUX1ZNC9J2 (Luteolin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE


  7 / 1296 MEDLINE  
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[PMID]:28602506
[Au] Autor:Wang J; Geng S; Wang B; Shao Q; Fang Y; Wei Y
[Ad] Endereço:State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 3(rd) Ring North East Road, Chaoyang District, Beijing 100029, China.
[Ti] Título:Magnetic nanoparticles and high-speed countercurrent chromatography coupled in-line and using the same solvent system for separation of quercetin-3-O-rutinoside, luteoloside and astragalin from a Mikania micrantha extract.
[So] Source:J Chromatogr A;1508:42-52, 2017 Jul 28.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A new in-line method of magnetic nanoparticles (MNPs) coupled with high-speed countercurrent chromatography (HSCCC) using a same solvent system during the whole separation process was established to achieve the rapid separation of flavonoids from Mikania micrantha. The adsorption and desorption capacities of five different MNPs for flavonoid standards and Mikania micrantha crude extract were compared and the most suitable magnetic nanoparticle Fe O @SiO @DIH@EMIMLpro was selected as the in-line MNP column. An in-line separation system was established by combining this MNP column with HSCCC through a six-way valve. The comparison between two solvent systems n-hexane-ethyl acetate-methanol-water (3:5:3:5, v/v) and ethyl acetate-methanol-water (25:1:25, v/v) showed that the latter solvent system was more suitable for simultaneously in-line separating three flavonoids quercetin-3-O-rutinoside, luteoloside and astragalin from Mikania micrantha. The purities of these three compounds with the ethyl acetate-methanol-water solvent system were 95.13%, 98.54% and 98.19% respectively. Results showed the established in-line separation system of MNP-HSCCC was efficient, recyclable and served to isolate potential flavonoids with similar polarities from natural complex mixtures. The in-line combination of magnetic nanoparticles with high-speed countercurrent chromatography eluting with the same solvent system during the whole separation process was established for the first time.
[Mh] Termos MeSH primário: Distribuição Contracorrente/métodos
Glucosídeos/isolamento & purificação
Quempferóis/isolamento & purificação
Luteolina/isolamento & purificação
Mikania/química
Extratos Vegetais/isolamento & purificação
Quercetina/análogos & derivados
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão/métodos
Distribuição Contracorrente/instrumentação
Glucosídeos/química
Quempferóis/química
Luteolina/química
Nanopartículas de Magnetita/química
Extratos Vegetais/química
Quercetina/química
Quercetina/isolamento & purificação
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucosides); 0 (Kaempferols); 0 (Magnetite Nanoparticles); 0 (Plant Extracts); 0 (quercetin-3-O-rutinoside); 98J6XDS46I (luteolin-7-glucoside); 9IKM0I5T1E (Quercetin); APM8UQ3Z9O (astragalin); KUX1ZNC9J2 (Luteolin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170613
[St] Status:MEDLINE


  8 / 1296 MEDLINE  
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[PMID]:28594846
[Au] Autor:Eudes A; Dutta T; Deng K; Jacquet N; Sinha A; Benites VT; Baidoo EEK; Richel A; Sattler SE; Northen TR; Singh S; Simmons BA; Loqué D
[Ad] Endereço:Joint BioEnergy Institute, EmeryStation East, Emeryville, California, United States of America.
[Ti] Título:SbCOMT (Bmr12) is involved in the biosynthesis of tricin-lignin in sorghum.
[So] Source:PLoS One;12(6):e0178160, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lignin in plant biomass represents a target for engineering strategies towards the development of a sustainable bioeconomy. In addition to the conventional lignin monomers, namely p-coumaryl, coniferyl and sinapyl alcohols, tricin has been shown to be part of the native lignin polymer in certain monocot species. Because tricin is considered to initiate the polymerization of lignin chains, elucidating its biosynthesis and mechanism of export to the cell wall constitute novel challenges for the engineering of bioenergy crops. Late steps of tricin biosynthesis require two methylation reactions involving the pathway intermediate selgin. It has recently been demonstrated in rice and maize that caffeate O-methyltransferase (COMT) involved in the synthesis syringyl (S) lignin units derived from sinapyl alcohol also participates in the synthesis of tricin in planta. In this work, we validate in sorghum (Sorghum bicolor L.) that the O-methyltransferase responsible for the production of S lignin units (SbCOMT / Bmr12) is also involved in the synthesis of lignin-linked tricin. In particular, we show that biomass from the sorghum bmr12 mutant contains lower level of tricin incorporated into lignin, and that SbCOMT can methylate the tricin precursors luteolin and selgin. Our genetic and biochemical data point toward a general mechanism whereby COMT is involved in the synthesis of both tricin and S lignin units.
[Mh] Termos MeSH primário: Vias Biossintéticas
Flavonoides/biossíntese
Lignina/biossíntese
Proteínas de Plantas/metabolismo
Sorghum/metabolismo
[Mh] Termos MeSH secundário: Biomassa
Celulose/metabolismo
Cromonas/metabolismo
Flavonoides/química
Lignina/química
Luteolina/metabolismo
Metanol/química
Metilação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromones); 0 (Flavonoids); 0 (Plant Proteins); 5627PY99ZO (tricetin); 9004-34-6 (Cellulose); 9005-53-2 (Lignin); D51JZL38TQ (tricin); KUX1ZNC9J2 (Luteolin); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170609
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178160


  9 / 1296 MEDLINE  
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[PMID]:28573229
[Au] Autor:Al-Rimawi F; Abu-Lafi S; Abbadi J; Alamarneh AAA; Sawahreh RA; Odeh I
[Ad] Endereço:Chemistry Department, Faculty of Science and Technology, Al-Quds University, P.O. Box 20002, Jerusalem, Palestine.
[Ti] Título:ANALYSIS OF PHENOLIC AND FLAVONOIDS OF WILD PLANT EXTRACTS BY LC/PDA AND LC/MS AND THEIR ANTIOXIDANT ACTIVITY.
[So] Source:Afr J Tradit Complement Altern Med;14(2):130-141, 2017.
[Is] ISSN:2505-0044
[Cp] País de publicação:Nigeria
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Ephedra is among Palestinian medicinal plants that are traditionally used in folkloric medicine for treating many diseases. Ephedra is known to have antibacterial and antioxidant effects. The goal of this study is to evaluate the antioxidant activity of different extracts from the plant growing wild in Palestine, and to analyze their phenolic and flavonoid constituents by HPLC/PDA and HPLC/MS. MATERIALS AND METHODS: Samples of the plant grown wild in Palestine were extracted with three different solvents namely, 100% water, 80% ethanol, and 100% ethanol. The extracts were analyzed for their total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (AA), as well as phenolic and flavonoids content by HPLC/PDA/MS. RESULTS: The results revealed that the polarity of the extraction solvent affects the TPC, TFC, and AA of extracts. It was found that both TPC and AA are highest for plant extracted with 80% ethanol, followed by 100% ethanol, and finally with 100% water. TFC however was highest in the following order: 100% ethanol > 80% ethanol > water. Pearson correlation indicated that there is a significant correlation between AA and TPC, but there is no correlation between AA and TFC. Simultaneous HPLC-PDA and UHPLC-MS analysis of the ethanolic plant extracts revealed the presence of Luteolin-7-O-glucuronide flavone, Myricetin 3-rhamnoside and some other major polyphenolic compounds that share myricetin skeleton. CONCLUSION: extract is rich in potent falvonoid glycosidic compounds as revealed by their similar overlaid UV-Vis spectra and UHPLC-MS results. On the basis of these findings, it is concluded that constitutes a natural source of potent antioxidants that may prevent many diseases and could be potentially used in food, cosmetics, and pharmaceutical products.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Ephedra/química
Flavonoides/farmacologia
Fenóis/farmacologia
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Antioxidantes/análise
Cromatografia Líquida de Alta Pressão
Flavonas/análise
Flavonas/farmacologia
Flavonoides/análise
Glicosídeos/análise
Glicosídeos/farmacologia
Luteolina/análise
Luteolina/farmacologia
Espectrometria de Massas/métodos
Oxirredução
Fenóis/análise
Extratos Vegetais/química
Polifenóis/análise
Polifenóis/farmacologia
Solventes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Flavones); 0 (Flavonoids); 0 (Glycosides); 0 (Phenols); 0 (Plant Extracts); 0 (Polyphenols); 0 (Solvents); 0 (luteolin-7-O-glucuronide); 76XC01FTOJ (myricetin); KUX1ZNC9J2 (Luteolin); S2V45N7G3B (flavone)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170816
[Lr] Data última revisão:
170816
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170603
[St] Status:MEDLINE
[do] DOI:10.21010/ajtcam.v14i2.14


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[PMID]:28429944
[Au] Autor:Lee D; Park HL; Lee SW; Bhoo SH; Cho MH
[Ad] Endereço:Graduate School of Biotechnology and ‡Crop Biotech Institute, Kyung Hee University , Yongin 17104, Republic of Korea.
[Ti] Título:Biotechnological Production of Dimethoxyflavonoids Using a Fusion Flavonoid O-Methyltransferase Possessing Both 3'- and 7-O-Methyltransferase Activities.
[So] Source:J Nat Prod;80(5):1467-1474, 2017 May 26.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Although they are less abundant in nature, methoxyflavonoids have distinct physicochemical and pharmacological properties compared to common nonmethylated flavonoids. Thus, enzymatic conversion and biotransformation using genetically engineered microorganisms of flavonoids have been attempted for the efficient production of methoxyflavonoids. Because of their regiospecificity, more than two flavonoid O-methyltransferases (FOMTs) and enzyme reactions are required to biosynthesize di(or poly)-methoxyflavonoids. For the one-step biotechnological production of bioactive di-O-methylflavonoids, we generated a multifunctional FOMT fusing a 3'-OMT (SlOMT3) and a 7-OMT (OsNOMT). The SlOMT3/OsNOMT fusion enzyme possessed both 3'- and 7-OMT activities to diverse flavonoid substrates, which were comparable to those of individual SlOMT3 and OsNOMT. The SlOMT3/OsNOMT enzyme also showed 3'- and 7-OMT activity for 7- or 3'-O-methylflavonoids, respectively, suggesting that the fusion enzyme can sequentially methylate flavonoids into di-O-methylflavonoids. The biotransformation of the flavonoids quercetin, luteolin, eriodictyol, and taxifolin using SlOMT3/OsNOMT-transformed Escherichia coli generated corresponding di-O-methylflavonoids, rhamnazin, velutin, 3',7-di-O-methyleriodictyol, and 3',7-di-O-methyltaxifolin, respectively. These results indicate that dimethoxyflavonoids may be efficiently produced from nonmethylated flavonoid precursors through a one-step biotransformation using the engineered E. coli harboring the SlOMT3/OsNOMT fusion gene.
[Mh] Termos MeSH primário: Escherichia coli/genética
Flavanonas/química
Flavonoides/metabolismo
Luteolina/química
Metiltransferases/metabolismo
Quercetina/análogos & derivados
[Mh] Termos MeSH secundário: Escherichia coli/química
Flavanonas/metabolismo
Flavonoides/química
Flavonoides/isolamento & purificação
Luteolina/metabolismo
Metilação
Metiltransferases/química
Estrutura Molecular
Quercetina/química
Quercetina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3',7-di-O-methyleriodictyol); 0 (3',7-di-O-methyltaxifolin); 0 (Flavanones); 0 (Flavonoids); 51857-11-5 (7-O-methyleriodictyol); 9IKM0I5T1E (Quercetin); 9SOB9E3987 (taxifolin); EC 2.1.1.- (Methyltransferases); KUX1ZNC9J2 (Luteolin); Q520486B8Y (eriodictyol)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170422
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.6b01164



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