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[PMID]:28934497
[Au] Autor:Wilson DM; Rieckher M; Williams AB; Schumacher B
[Ad] Endereço:Laboratory of Molecular Gerontology, National Institute on Aging, Intramural Research Program, National Institutes of Health, Baltimore, MD 21224, USA.
[Ti] Título:Systematic analysis of DNA crosslink repair pathways during development and aging in Caenorhabditis elegans.
[So] Source:Nucleic Acids Res;45(16):9467-9480, 2017 Sep 19.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:DNA interstrand crosslinks (ICLs) are generated by endogenous sources and chemotherapeutics, and pose a threat to genome stability and cell survival. Using Caenorhabditis elegans mutants, we identify DNA repair factors that protect against the genotoxicity of ICLs generated by trioxsalen/ultraviolet A (TMP/UVA) during development and aging. Mutations in nucleotide excision repair (NER) components (e.g. XPA-1 and XPF-1) imparted extreme sensitivity to TMP/UVA relative to wild-type animals, manifested as developmental arrest, defects in adult tissue morphology and functionality, and shortened lifespan. Compensatory roles for global-genome (XPC-1) and transcription-coupled (CSB-1) NER in ICL sensing were exposed. The analysis also revealed contributions of homologous recombination (BRC-1/BRCA1), the MUS-81, EXO-1, SLX-1 and FAN-1 nucleases, and the DOG-1 (FANCJ) helicase in ICL resolution, influenced by the replicative-status of the cell/tissue. No obvious or critical role in ICL repair was seen for non-homologous end-joining (cku-80) or base excision repair (nth-1, exo-3), the Fanconi-related proteins BRC-2 (BRCA2/FANCD1) and FCD-2 (FANCD2), the WRN-1 or HIM-6 (BLM) helicases, or the GEN-1 or MRT-1 (SNM1) nucleases. Our efforts uncover replication-dependent and -independent ICL repair networks, and establish nematodes as a model for investigating the repair and consequences of DNA crosslinks in metazoan development and in adult post-mitotic and proliferative germ cells.
[Mh] Termos MeSH primário: Envelhecimento
Caenorhabditis elegans/fisiologia
Reparo do DNA
[Mh] Termos MeSH secundário: Envelhecimento/efeitos dos fármacos
Envelhecimento/fisiologia
Envelhecimento/efeitos da radiação
Animais
Caenorhabditis elegans/efeitos dos fármacos
Caenorhabditis elegans/crescimento & desenvolvimento
Caenorhabditis elegans/efeitos da radiação
Proteínas de Caenorhabditis elegans/genética
DNA/química
Reparo do DNA/efeitos dos fármacos
Reparo do DNA/efeitos da radiação
Feminino
Recombinação Homóloga
Masculino
Mutação
Trioxsaleno/farmacologia
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Caenorhabditis elegans Proteins); 9007-49-2 (DNA); Y6UY8OV51T (Trioxsalen)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170922
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx660


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[PMID]:28130555
[Au] Autor:Lehmann J; Seebode C; Smolorz S; Schubert S; Emmert S
[Ad] Endereço:Clinic and Policlinic for Dermatology and Venereology, University Medical Centre Rostock, Strempelstrasse 13, 18057, Rostock, Germany.
[Ti] Título:XPF knockout via CRISPR/Cas9 reveals that ERCC1 is retained in the cytoplasm without its heterodimer partner XPF.
[So] Source:Cell Mol Life Sci;74(11):2081-2094, 2017 Jun.
[Is] ISSN:1420-9071
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The XPF/ERCC1 heterodimeric complex is essentially involved in nucleotide excision repair (NER), interstrand crosslink (ICL), and double-strand break repair. Defects in XPF lead to severe diseases like xeroderma pigmentosum (XP). Up until now, XP-F patient cells have been utilized for functional analyses. Due to the multiple roles of the XPF/ERCC1 complex, these patient cells retain at least one full-length allele and residual repair capabilities. Despite the essential function of the XPF/ERCC1 complex for the human organism, we successfully generated a viable immortalised human XPF knockout cell line with complete loss of XPF using the CRISPR/Cas9 technique in fetal lung fibroblasts (MRC5Vi cells). These cells showed a markedly increased sensitivity to UVC, cisplatin, and psoralen activated by UVA as well as reduced repair capabilities for NER and ICL repair as assessed by reporter gene assays. Using the newly generated knockout cells, we could show that human XPF is markedly involved in homologous recombination repair (HRR) but dispensable for non-homologous end-joining (NHEJ). Notably, ERCC1 was not detectable in the nucleus of the XPF knockout cells indicating the necessity of a functional XPF/ERCC1 heterodimer to allow ERCC1 to enter the nucleus. Overexpression of wild-type XPF could reverse this effect as well as the repair deficiencies.
[Mh] Termos MeSH primário: Sistemas CRISPR-Cas/genética
Citoplasma/metabolismo
Proteínas de Ligação a DNA/metabolismo
Endonucleases/metabolismo
Técnicas de Inativação de Genes
Multimerização Proteica
[Mh] Termos MeSH secundário: Sequência de Bases
Linhagem Celular
Cisplatino/farmacologia
Citoplasma/efeitos dos fármacos
Citoplasma/efeitos da radiação
Dano ao DNA
Reparo do DNA/efeitos dos fármacos
Reparo do DNA/genética
Reparo do DNA/efeitos da radiação
Genes Reporter
Recombinação Homóloga/genética
Seres Humanos
Multimerização Proteica/efeitos dos fármacos
Multimerização Proteica/efeitos da radiação
Toxinas Biológicas/metabolismo
Trioxsaleno/farmacologia
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA-Binding Proteins); 0 (Toxins, Biological); 0 (xeroderma pigmentosum group F protein); EC 3.1.- (ERCC1 protein, human); EC 3.1.- (Endonucleases); Q20Q21Q62J (Cisplatin); Y6UY8OV51T (Trioxsalen)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170129
[St] Status:MEDLINE
[do] DOI:10.1007/s00018-017-2455-7


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[PMID]:25199638
[Au] Autor:Kobori A; Yamayoshi A; Murakami A
[Ad] Endereço:Kyoto Institute of Technology, Graduate School of Science and Technology, Department of Biomolecular Engineering, Matsugasaki, Sakyo-ku, Kyoto, Japan.
[Ti] Título:Synthesis of Oligonucleotides Containing 4,5',8-Trimethylpsoralen at the 2'-O Position and Their Cross-Linking Properties with RNAs.
[So] Source:Curr Protoc Nucleic Acid Chem;58:5.15.1-15, 2014 Sep 08.
[Is] ISSN:1934-9289
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:4,5',8-Trimethylpsoralen-conjugated oligonucleotides have been used in the study of photo-cross-linking with target oligonucleotides and in the field of the photodynamic therapy. This unit describes synthetic procedures for oligonucleotides using 2'-O-methylphosphoramidite units and an adenosine phosphoramidite unit containing a 4,5',8-trimethylpsoralen derivative attached at the 2' position of an adenosine sugar moiety via an ethoxymethylene linkage. Procedures for obtaining the photo-cross-linking efficiency of 2'-O-methyloligonucleotides containing a 4,5',8-trimethylpsoralen derivative with a target oligonucleotide under UV irradiation conditions are also described, together with the procedure for preparation of (32)P-radiolabeled RNA.
[Mh] Termos MeSH primário: Reagentes para Ligações Cruzadas/química
Oligonucleotídeos/química
Oligonucleotídeos/síntese química
RNA/química
Trioxsaleno/química
[Mh] Termos MeSH secundário: Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Cross-Linking Reagents); 0 (Oligonucleotides); 63231-63-0 (RNA); Y6UY8OV51T (Trioxsalen)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:161021
[Lr] Data última revisão:
161021
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140910
[St] Status:MEDLINE
[do] DOI:10.1002/0471142700.nc0515s58


  4 / 486 MEDLINE  
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[PMID]:25183819
[Au] Autor:Nilsen TW
[Ti] Título:Detecting RNA-RNA interactions using psoralen derivatives.
[So] Source:Cold Spring Harb Protoc;2014(9):996-1000, 2014 Sep 02.
[Is] ISSN:1559-6095
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Psoralens are tricyclic compounds that intercalate into double-stranded DNA or RNA and, on irradiation with long-wavelength (365-nm) UV light, covalently link pyrimidines on adjacent strands. More rarely, psoralen cross-links can be observed at the ends of helices (i.e., double-stranded-single-stranded boundaries). Although psoralens can, in some instances, cross-link protein to RNA, their primary application is to detect RNA-RNA base-pairing interactions. The most useful psoralen derivative is 4'-aminomethyl trioxsalen (AMT), which is soluble in H2O. This protocol describes the use of AMT to detect RNA-RNA interactions in tissue culture cells or in extracts. Cross-linked RNAs are detectable by their reduced mobility in polyacrylamide gels. Cross-links can be reversed by exposure to short-wavelength (254 nm) UV light.
[Mh] Termos MeSH primário: Reagentes para Ligações Cruzadas/farmacologia
RNA/efeitos dos fármacos
RNA/metabolismo
Trioxsaleno/farmacologia
[Mh] Termos MeSH secundário: Núcleo Celular/efeitos dos fármacos
Núcleo Celular/metabolismo
DNA/efeitos dos fármacos
Células HeLa
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cross-Linking Reagents); 63231-63-0 (RNA); 9007-49-2 (DNA); Y6UY8OV51T (Trioxsalen)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:140903
[Lr] Data última revisão:
140903
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140904
[St] Status:MEDLINE
[do] DOI:10.1101/pdb.prot080861


  5 / 486 MEDLINE  
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[PMID]:24613935
[Au] Autor:Kage-Nakadai E; Imae R; Yoshina S; Mitani S
[Ad] Endereço:Department of Physiology, Tokyo Women's Medical University, School of Medicine, 8-1, Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan; The OCU Advanced Research Institute for Natural Science and Technology, Osaka City University, 3-3-138, Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan. Electronic addre
[Ti] Título:Methods for single/low-copy integration by ultraviolet and trimethylpsoralen treatment in Caenorhabditis elegans.
[So] Source:Methods;68(3):397-402, 2014 Aug 01.
[Is] ISSN:1095-9130
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Single/low-copy transgene integration is essential for avoiding overexpression, ectopic expression and gene silencing in the germline. Here, we present an overview of a method that uses ultraviolet and trimethylpsoralen (UV/TMP) to generate single/low-copy gene integrations in Caenorhabditis elegans. Single/low-copy transgenes from extrachromosomal arrays are integrated into the genome using positive selection based on temperature sensitivity with a vps-45 rescue fragment and negative selection based on benzimidazole sensitivity with a ben-1 rescue fragment. The copy number of the integrated transgenes is determined using quantitative PCR. Our UV/TMP integration method, which is based on familiar extrachromosomal transgenics, provides a simple approach for generating single/low-copy gene integrations.
[Mh] Termos MeSH primário: Animais Geneticamente Modificados/genética
Caenorhabditis elegans/genética
Transgenes
[Mh] Termos MeSH secundário: Animais
Genoma/efeitos dos fármacos
Genoma/efeitos da radiação
Transgenes/efeitos dos fármacos
Transgenes/efeitos da radiação
Trioxsaleno/farmacologia
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
Y6UY8OV51T (Trioxsalen)
[Em] Mês de entrada:1504
[Cu] Atualização por classe:140728
[Lr] Data última revisão:
140728
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140312
[St] Status:MEDLINE


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[PMID]:24362322
[Au] Autor:Silva EB; Barbosa IJ; Barreto HM; Siqueira-Júnior JP
[Ad] Endereço:Departamento de Biologia Molecular, Universidade Federal da Paraíba, João Pessoa, Brazil. Electronic address: manubatistapb@gmail.com.
[Ti] Título:Modulation of the UVB-induced lethality by furocoumarins in Staphylococcus aureus.
[So] Source:J Photochem Photobiol B;130:260-3, 2014 Jan 05.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Furocumarins (FCs) are photoactive compounds capable of binding to DNA, and once excited by UVA light (∼365nm), they form photoadducts which can lead to mutagenicity and lethality. However, the biological effects of FCs combined with UVB light (312nm) is still little investigated. In the present study, the lethal effect of UVB light alone and combined with different concentrations of 8-methoxypsoralen (8-MOP), 4,5',8-trimethylpsoralen (TMP) and 3-carbethoxypsoralen (3-CPs) was evaluated in a strain of Staphylococcus aureus. 8-MOP-UVB and TMP-UVB were more effective in inducing lethality compared to UVB alone, indicating that these FCs act as photosensitizing agents for UVB. The increase in concentration of 8-MOP resulted in a greater mortality. On the contrary, a decrease in mortality was found with an increase in TMP concentration. 3-CPs protected bacteria against damage induced by UVB, which can be attributed to the inhibition of cyclobutyl pyrimidine dimer formation. The different modulatory effects on lethality induced by UVB shown by the FCs tested could be related to differences in the specificity of each compound for particular nucleotide sequences, as well as other chemical characteristics of each molecule could influence the number and types of adducts formed, contributing to the photosensitizing or photoprotective effects observed.
[Mh] Termos MeSH primário: Metoxaleno/farmacologia
Fármacos Fotossensibilizantes/farmacologia
Staphylococcus aureus/efeitos dos fármacos
Trioxsaleno/farmacologia
Raios Ultravioleta
[Mh] Termos MeSH secundário: Furocumarinas/farmacologia
Staphylococcus aureus/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Furocoumarins); 0 (Photosensitizing Agents); BB04Z808HM (3-carbethoxypsoralen); U4VJ29L7BQ (Methoxsalen); Y6UY8OV51T (Trioxsalen)
[Em] Mês de entrada:1411
[Cu] Atualização por classe:141120
[Lr] Data última revisão:
141120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131224
[St] Status:MEDLINE


  7 / 486 MEDLINE  
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[PMID]:24079701
[Au] Autor:Parnami N; Garg T; Rath G; Goyal AK
[Ad] Endereço:Department of Pharmaceutics, ISF College of Pharmacy , Moga, Punjab , India.
[Ti] Título:Development and characterization of nanocarriers for topical treatment of psoriasis by using combination therapy.
[So] Source:Artif Cells Nanomed Biotechnol;42(6):406-12, 2014 Dec.
[Is] ISSN:2169-141X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Psoriasis is an autoimmune, chronic, inflammatory skin disease characterized by epidermal hyperplasia, proliferation of blood vessels, and infiltration of leukocytes in dermis and epidermis. Several immunosuppressants such as methotrexate (MXT) and cyclosporine are used but they are associated with adverse effects due to down regulation of immune system. Numerous approaches have been explored to overcome the problems of conventional topical system such as high frequency of application, impermeability to skin barrier, and limited efficacy. Photodynamic therapy is another non-invasive technique currently used for skin diseases. The combination of two drugs is also commonly observed to achieve more effective therapy. In the present study, antipsoriatic activity of niosomal formulations for the treatment of psoriasis in combination with narrow and broad band UV radiation had been explored in experimental animal model.
[Mh] Termos MeSH primário: Lipossomos/administração & dosagem
Nanosferas/administração & dosagem
Paraceratose/terapia
Psoríase/terapia
[Mh] Termos MeSH secundário: Administração Tópica
Animais
Terapia Combinada
Modelos Animais de Doenças
Seres Humanos
Metotrexato/administração & dosagem
Camundongos Endogâmicos
Procedimentos Ortoceratológicos
Paraceratose/tratamento farmacológico
Paraceratose/radioterapia
Fotoquimioterapia
Psoríase/tratamento farmacológico
Psoríase/radioterapia
Trioxsaleno/administração & dosagem
Terapia Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Liposomes); Y6UY8OV51T (Trioxsalen); YL5FZ2Y5U1 (Methotrexate)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:141114
[Lr] Data última revisão:
141114
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131002
[St] Status:MEDLINE
[do] DOI:10.3109/21691401.2013.837474


  8 / 486 MEDLINE  
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[PMID]:24010339
[Au] Autor:Ma H; Ma Q; Li W; Meng X; Li J; Bai H; Jiao Y; Zhang X
[Ad] Endereço:Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
[Ti] Título:[Simultaneous determination of eight furocoumarines in cosmetics by high performance liquid chromatography and verification by liquid chromatography-tandem mass spectrometry].
[So] Source:Se Pu;31(5):416-22, 2013 May.
[Is] ISSN:1000-8713
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:A method using high performance liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the simultaneous determination of eight furocoumarines (8-hydroxypsoralen, psoralen, isopsoralen, 8-methoxypsoralen, 5-methoxypsoralen, trioxsalen, imperatorin and isoimperatorin) in cosmetics. The cosmetic samples, including cream, lotion, shampoo, powder and lipstick, were supersonically extracted with appropriate solvents. The extract was centrifuged, and the supernatant was filtered through a membrane, and then separated on an Agilent Zorbax SB-Phenyl chromatographic column (250 mm x 4.6 mm, 5 microm) by gradient elution at a flow rate of 1.0 mL/min with methanol-acetonitrile-water as mobile phases. The column temperature was set at 30 degrees C. The wavelength of detection was 250 nm. The limits of quantification (LOQs) were 0.25 mg/kg for 8-hydroxypsoralen and 0.5 mg/kg for psoralen, isopsoralen, 8-methoxypsoralen, 5-methoxypsoralen, trioxsalen, imperatorin and isoimperatorin. The recoveries at three spiked levels were in the range of 85.0% - 105.8% with the relative standard deviations (RSDs) of 0.41% - 7.90%. The intra-day precision (n=6) was less than 1%, and the inter-day precision (n = 6) was less than 2% for the peak areas of the eight furocoumarines in a mixed standard solution. The method is accurate, simple, rapid and suitable for the determination of the eight furocoumarines in various cosmetic samples.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão
Cosméticos/análise
Furocumarinas/análise
Espectrometria de Massas em Tandem
[Mh] Termos MeSH secundário: Ficusina
Metoxaleno/análogos & derivados
Trioxsaleno
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cosmetics); 0 (Furocoumarins); 0ZMV066080 (isoimperatorin); 2009-24-7 (xanthotoxol); 4FVK84C92X (5-methoxypsoralen); CZZ080D7BD (angelicin); K713N25C78 (imperatorin); KTZ7ZCN2EX (Ficusin); U4VJ29L7BQ (Methoxsalen); Y6UY8OV51T (Trioxsalen)
[Em] Mês de entrada:1507
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130910
[St] Status:MEDLINE


  9 / 486 MEDLINE  
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[PMID]:23835446
[Au] Autor:Raviprakash K; Sun P; Raviv Y; Luke T; Martin N; Kochel T
[Ad] Endereço:Viral & Rickettsial Diseases Department; Naval Medical Research Center; Silver Spring, MD USA.
[Ti] Título:Dengue virus photo-inactivated in presence of 1,5-iodonaphthylazide (INA) or AMT, a psoralen compound (4'-aminomethyl-trioxsalen) is highly immunogenic in mice.
[So] Source:Hum Vaccin Immunother;9(11):2336-41, 2013 Nov.
[Is] ISSN:2164-554X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Two novel methods of dengue virus inactivation using iodonaphthyl azide (INA) and aminomethyl trioxsalen (AMT) were compared with traditional virus inactivation by formaldehyde. The AMT inactivated dengue-2 virus retained its binding to a panel of 5 monoclonal antibodies specific for dengue-2 envelope protein, whereas inactivation by formaldehyde and INA led to 30-50% decrease in binding. All three inactivated viruses elicited high level virus neutralizing antibodies in vaccinated mice. However, only mice vaccinated with AMT inactivated virus mounted T cell responses similar to live, uninactivated virus.
[Mh] Termos MeSH primário: Azidas/farmacologia
Vacinas contra Dengue/imunologia
Vírus da Dengue/efeitos dos fármacos
Vírus da Dengue/imunologia
Desinfetantes/farmacologia
Trioxsaleno/análogos & derivados
Inativação de Vírus
[Mh] Termos MeSH secundário: Animais
Anticorpos Neutralizantes/sangue
Anticorpos Antivirais/sangue
Vacinas contra Dengue/administração & dosagem
Formaldeído/farmacologia
Luz
Camundongos Endogâmicos BALB C
Linfócitos T/imunologia
Trioxsaleno/farmacologia
Vacinas de Produtos Inativados/administração & dosagem
Vacinas de Produtos Inativados/imunologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Antibodies, Neutralizing); 0 (Antibodies, Viral); 0 (Azides); 0 (Dengue Vaccines); 0 (Disinfectants); 0 (Vaccines, Inactivated); 1HG84L3525 (Formaldehyde); 63785-44-4 (iodonaphthylazide); 64358-50-5 (aminomethyltrioxsalen); Y6UY8OV51T (Trioxsalen)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130710
[St] Status:MEDLINE


  10 / 486 MEDLINE  
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[PMID]:23825018
[Au] Autor:Hartman MR; Yang D; Tran TN; Lee K; Kahn JS; Kiatwuthinon P; Yancey KG; Trotsenko O; Minko S; Luo D
[Ad] Endereço:Department of Biological & Environmental Engineering, Cornell University, Ithaca, NY 14853, USA.
[Ti] Título:Thermostable branched DNA nanostructures as modular primers for polymerase chain reaction.
[So] Source:Angew Chem Int Ed Engl;52(33):8699-702, 2013 Aug 12.
[Is] ISSN:1521-3773
[Cp] País de publicação:Germany
[La] Idioma:eng
[Mh] Termos MeSH primário: Materiais Biocompatíveis/química
Primers do DNA/química
DNA/química
Hidrogéis/química
Nanoestruturas/química
Reação em Cadeia da Polimerase/métodos
[Mh] Termos MeSH secundário: Materiais Biocompatíveis/metabolismo
Reagentes para Ligações Cruzadas
DNA/metabolismo
Primers do DNA/metabolismo
Ficusina/química
Hidrogéis/metabolismo
Modelos Moleculares
Estrutura Molecular
Nanotecnologia
Conformação de Ácido Nucleico
Temperatura Ambiente
Trioxsaleno/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Cross-Linking Reagents); 0 (DNA Primers); 0 (Hydrogels); 9007-49-2 (DNA); KTZ7ZCN2EX (Ficusin); Y6UY8OV51T (Trioxsalen)
[Em] Mês de entrada:1310
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130705
[St] Status:MEDLINE
[do] DOI:10.1002/anie.201302175



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