[PMID]: | 28514904 |
[Au] Autor: | Park SH; Sung YY; Nho KJ; Kim DS; Kim HK |
[Ad] Endereço: | * Mibyeong Research Center, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon 305-811, Republic of Korea. |
[Ti] Título: | Effects of Viola mandshurica on Atherosclerosis and Hepatic Steatosis in ApoE[Formula: see text] via the AMPK Pathway. |
[So] Source: | Am J Chin Med;45(4):757-772, 2017. |
[Is] ISSN: | 0192-415X |
[Cp] País de publicação: | Singapore |
[La] Idioma: | eng |
[Ab] Resumo: | Atherosclerosis was previously thought to be a disease that primarily involves lipid accumulation in the arterial wall. In this report, we investigated the effect of Viola mandshurica W. Becker (V. mandshurica) water extract on atherosclerosis in apolipoprotein E deficient (ApoE[Formula: see text]) mice. The administration of V. mandshurica to high-fat diet-fed mice reduced body weight, liver weight, and serum levels of lipids (total cholesterol, low-density lipoprotein-cholesterol, triglycerides), glucose, alanine transaminase, and aspartate transaminase. Histopathologic analyses of the aorta and liver revealed that V. mandshurica attenuated atherosclerotic lesions and reduced lipid accumulation, inflammatory responses and fatty acid synthesis. V. mandshurica also increased phosphorylation of adenosine monophosphate-activated protein kinase (AMPK), thereby reducing acetyl-CoA carboxylase (ACC) in liver tissue and inhibiting sterol regulatory element-binding protein 1c (SREBP-1c). V. mandshurica reduced protein expression levels of adhesion molecules (intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and E-selectin) as well as ACC, fatty acid synthase, and SREBP-1c. In addition, quantitative analysis of V. mandshurica by high-performance liquid chromatography revealed the presence of esculetin and scopoletin. Esculetin and scopoletin reduced adhesion molecules in human aortic smooth muscle cells. Our results indicate that the anti-atherosclerotic effects of V. mandshurica may be associated with activation of the AMPK pathway. Therefore, AMPK-dependent phosphorylation of SREBP-1c by V. mandshurica may be an effective therapeutic strategy for combatting atherosclerosis and hepatic steatosis. |
[Mh] Termos MeSH primário: |
Proteínas Quinases Ativadas por AMP/metabolismo Aterosclerose/tratamento farmacológico Aterosclerose/etiologia Fígado Gorduroso/tratamento farmacológico Fígado Gorduroso/etiologia Terapia de Alvo Molecular Fitoterapia Extratos Vegetais/farmacologia Extratos Vegetais/uso terapêutico Viola/química
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[Mh] Termos MeSH secundário: |
Acetil-CoA Carboxilase/metabolismo Animais Molécula 1 de Adesão Celular Moléculas de Adesão Celular/metabolismo Modelos Animais de Doenças Imunoglobulinas/metabolismo Metabolismo dos Lipídeos/efeitos dos fármacos Fígado/metabolismo Masculino Camundongos Endogâmicos C57BL Fosforilação/efeitos dos fármacos Extratos Vegetais/química Escopoletina/isolamento & purificação Escopoletina/farmacologia Escopoletina/uso terapêutico Transdução de Sinais Proteína de Ligação a Elemento Regulador de Esterol 1/metabolismo Umbeliferonas/isolamento & purificação Umbeliferonas/farmacologia Umbeliferonas/uso terapêutico
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Cadm1 protein, mouse); 0 (Cell Adhesion Molecule-1); 0 (Cell Adhesion Molecules); 0 (Immunoglobulins); 0 (Plant Extracts); 0 (Srebf1 protein, mouse); 0 (Sterol Regulatory Element Binding Protein 1); 0 (Umbelliferones); EC 2.7.11.31 (AMP-Activated Protein Kinases); EC 6.4.1.2 (Acetyl-CoA Carboxylase); KLF1HS0SXJ (Scopoletin); SM2XD6V944 (esculetin) |
[Em] Mês de entrada: | 1709 |
[Cu] Atualização por classe: | 171116 |
[Lr] Data última revisão:
| 171116 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170519 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1142/S0192415X17500409 |
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