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[PMID]: 29329333 [Au] Autor: Mohri S; Takahashi H; Sakai M; Takahashi S; Waki N; Aizawa K; Suganuma H; Ara T; Matsumura Y; Shibata D; Goto T; Kawada T [Ad] Endereço: Laboratory of Molecular Function of Food, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan. [Ti] Título: Wide-range screening of anti-inflammatory compounds in tomato using LC-MS and elucidating the mechanism of their functions. [So] Source: PLoS One;13(1):e0191203, 2018. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Obesity-induced chronic inflammation is a key factor in type 2 diabetes. A vicious cycle involving pro-inflammatory mediators between adipocytes and macrophages is a common cause of chronic inflammation in the adipose tissue. Tomato is one of the most popular vegetables and is associated with a reduced risk of diabetes. However, the molecular mechanism underlying the effect of tomato on diabetes is unclear. In this study, we focused on anti-inflammatory compounds in tomato. We found that the extract of tomato reduced plasma glucose and inflammatory markers in mice. We screened anti-inflammatory fractions in tomato using lipopolysaccharide-stimulated RAW264.7 macrophages, and active compounds were estimated by liquid chromatography-mass spectrometry over a wide range. Surprisingly, a large number of compounds including oxylipin and coumarin derivatives were estimated as anti-inflammatory compounds. Especially, 9-oxo-octadecadienoic acid and daphnetin suppressed pro-inflammatory cytokines in RAW264.7 macrophages inhibiting mitogen-activated protein kinase phosphorylation and inhibitor of kappa B α protein degradation. These findings suggest that tomato containing diverse anti-inflammatory compounds ameliorates chronic inflammation in obese adipose tissue. [Mh] Termos MeSH primário: Anti-Inflamatórios/isolamento & purificação Anti-Inflamatórios/farmacologia Lycopersicon esculentum/química [Mh] Termos MeSH secundário: Tecido Adiposo/efeitos dos fármacos Tecido Adiposo/metabolismo Animais Anti-Inflamatórios/química Glicemia/metabolismo Cromatografia Líquida de Alta Pressão Cumarínicos/química Cumarínicos/isolamento & purificação Cumarínicos/farmacologia Citocinas/metabolismo Avaliação Pré-Clínica de Medicamentos Sistema de Sinalização das MAP Quinases/efeitos dos fármacos Macrófagos/efeitos dos fármacos Macrófagos/metabolismo Masculino Camundongos Camundongos Endogâmicos C57BL Óxido Nítrico/biossíntese Obesidade/tratamento farmacológico Obesidade/metabolismo Oxilipinas/química Oxilipinas/isolamento & purificação Oxilipinas/farmacologia Extratos Vegetais/química Extratos Vegetais/isolamento & purificação Extratos Vegetais/farmacologia Células RAW 264.7 Espectrometria de Massas por Ionização por Electrospray Umbeliferonas/química Umbeliferonas/isolamento & purificação Umbeliferonas/farmacologia [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Anti-Inflammatory Agents); 0 (Blood Glucose); 0 (Coumarins); 0 (Cytokines); 0 (Oxylipins); 0 (Plant Extracts); 0 (Umbelliferones); 31C4KY9ESH (Nitric Oxide); XC84571RD2 (daphnetin) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180226 [Lr] Data última revisão: 180226 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180113 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0191203

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[PMID]: 29217385 [Au] Autor: Witaicenis A; de Oliveira ECS; Tanimoto A; Zorzella-Pezavento SFG; de Oliveira SL; Sartori A; Di Stasi LC [Ad] Endereço: Universidade Estadual Paulista (UNESP), Institute of Biosciences, Department of Pharmacology, Câmpus Botucatu, SP, Brazil. Electronic address: aline.wit@ibb.unesp.br. [Ti] Título: 4-methylesculetin, a coumarin derivative, ameliorates dextran sulfate sodium-induced intestinal inflammation. [So] Source: Chem Biol Interact;280:59-63, 2018 Jan 25. [Is] ISSN: 1872-7786 [Cp] País de publicação: Ireland [La] Idioma: eng [Ab] Resumo: 4-methylesculetin is one of the coumarin derivatives with great anti-oxidant and anti-inflammatory activities. Recent studies have shown that 4-methylesculetin has a promising potentiality to treat inflammatory diseases, especially those related to reactive oxygen species, as inflammatory bowel disease. Based on this, the present study aims to investigate the intestinal anti-inflammatory activity of 4-methylesculetin in dextran sulfate sodium (DSS) model. For this purpose, mice received DSS 5% for 5 days followed by 2 days of filtered tap water. Treated groups received orally 5 or 25 mg/kg of 4-methylesculetin daily since the first day. Macroscopic, microscopic and biochemical parameters were evaluated. 4-methylesculetin (25 mg/kg) improved microscopic parameters, decreased MPO activity, reduced the colonic levels of IL-6 and counteracted GSH depletion when compared with DSS-control group. Our results show the intestinal anti-inflammatory activity of 4-methylesculetin in DSS model, which is related to its antioxidant and anti-inflammatory properties. This way, 4-methylesculetin, is a new potential compound for treatment of both types of IBD. [Mh] Termos MeSH primário: Colite/induzido quimicamente Colo/efeitos dos fármacos Cumarínicos/farmacologia Sulfato de Dextrana Umbeliferonas/farmacologia [Mh] Termos MeSH secundário: Animais Colite/patologia Colite/prevenção & controle Colo/metabolismo Colo/patologia Cumarínicos/química Cumarínicos/uso terapêutico Ensaio de Imunoadsorção Enzimática Glutationa/metabolismo Interleucina-17/análise Interleucina-6/análise Masculino Camundongos Peroxidase/metabolismo Fator de Necrose Tumoral alfa/análise Umbeliferonas/química Umbeliferonas/uso terapêutico [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Coumarins); 0 (Interleukin-17); 0 (Interleukin-6); 0 (Tumor Necrosis Factor-alpha); 0 (Umbelliferones); 132H8N0A91 (4-methylesculetin); 9042-14-2 (Dextran Sulfate); EC 1.11.1.7 (Peroxidase); GAN16C9B8O (Glutathione) [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180129 [Lr] Data última revisão: 180129 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171209 [St] Status: MEDLINE

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[PMID]: 28470863 [Au] Autor: Ravikumar G; Bagheri M; Saini DK; Chakrapani H [Ad] Endereço: Department of Chemistry, Indian Institute of Science Education and Research, Dr. Homi Bhabha Road, Pune, 411008, Maharashtra, India. [Ti] Título: FLUORO/NO: A Nitric Oxide Donor with a Fluorescence Reporter. [So] Source: Chembiochem;18(15):1529-1534, 2017 08 04. [Is] ISSN: 1439-7633 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: Nitric oxide (NO) plays significant signalling roles in cells; the controlled generation of NO is of therapeutic relevance. Although a number of methods for the delivery and detection of NO are available, these events are typically mutually exclusive. Furthermore, the efficiency of delivery of NO can be compromised by detection technologies that consume NO. Here, we report FLUORO/NO, an esterase-activated diazeniumdiolate-based NO donor with an in-built fluorescence reporter. We demonstrate that this compound is capable of enhancing NO within cells in a dose-dependent manner, accompanied by a similar increase in fluorescence. The compatibility of this tool to study NO-mediated signalling as well as NO-mediated stress is demonstrated. FLUORO/NO is a convenient tool that shows NO-like activity and allows monitoring of NO release. This tool will help interrogate the redox biology of NO. [Mh] Termos MeSH primário: Cumarínicos/farmacologia Doadores de Óxido Nítrico/farmacologia Triazenos/farmacologia Triazinas/farmacologia Umbeliferonas/farmacologia [Mh] Termos MeSH secundário: Carboxilesterase/metabolismo Cumarínicos/síntese química Dano ao DNA Fluorescência Células HEK293 Células HeLa Seres Humanos Óxido Nítrico/metabolismo Doadores de Óxido Nítrico/síntese química Nitritos/análise Guanilil Ciclase Solúvel/metabolismo Estereoisomerismo Triazenos/síntese química Triazinas/síntese química Umbeliferonas/síntese química Valeratos/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Coumarins); 0 (FLUORO-NO compound); 0 (Nitric Oxide Donors); 0 (Nitrites); 0 (Triazenes); 0 (Triazines); 0 (Umbelliferones); 0 (Valerates); 31C4KY9ESH (Nitric Oxide); EC 3.1.1.1 (Carboxylesterase); EC 4.6.1.2 (Soluble Guanylyl Cyclase) [Em] Mês de entrada: 1712 [Cu] Atualização por classe: 180122 [Lr] Data última revisão: 180122 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170505 [St] Status: MEDLINE [do] DOI: 10.1002/cbic.201700155

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[PMID]: 28929747 [Au] Autor: Hopkins DH; Fraser NJ; Mabbitt PD; Carr PD; Oakeshott JG; Jackson CJ [Ad] Endereço: Research School of Chemistry, Australian National University , Canberra, Australian Capital Territory 0200, Australia. [Ti] Título: Structure of an Insecticide Sequestering Carboxylesterase from the Disease Vector Culex quinquefasciatus: What Makes an Enzyme a Good Insecticide Sponge? [So] Source: Biochemistry;56(41):5512-5525, 2017 Oct 17. [Is] ISSN: 1520-4995 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Carboxylesterase (CBE)-mediated metabolic resistance to organophosphate and carbamate insecticides is a major problem for the control of insect disease vectors, such as the mosquito. The most common mechanism involves overexpression of CBEs that bind to the insecticide with high affinity, thereby sequestering them before they can interact with their target. However, the absence of any structure for an insecticide-sequestering CBE limits our understanding of the molecular basis for this process. We present the first structure of a CBE involved in sequestration, Cqestß2 , from the mosquito disease vector Culex quinquefasciatus. Lysine methylation was used to obtain the crystal structure of Cqestß2 , which adopts a canonical α/ß-hydrolase fold that has high similarity to the target of organophosphate and carbamate insecticides, acetylcholinesterase. Sequence similarity networks of the insect carboxyl/cholinesterase family demonstrate that CBEs associated with metabolic insecticide resistance across many species share a level of similarity that distinguishes them from a variety of other classes. This is further emphasized by the structural similarities and differences in the binding pocket and active site residues of Cqestß2 and other insect carboxyl/cholinesterases. Stopped-flow and steady-state inhibition studies support a major role for Cqestß2 in organophosphate resistance and a minor role in carbamate resistance. Comparison with another isoform associated with insecticide resistance, Cqestß1, showed both enzymes have similar affinity to insecticides, despite 16 amino acid differences between the two proteins. This provides a molecular understanding of pesticide sequestration by insect CBEs and could facilitate the design of CBE-specific inhibitors to circumvent this resistance mechanism in the future. [Mh] Termos MeSH primário: Carboxilesterase/metabolismo Culex/enzimologia Proteínas de Insetos/metabolismo Inseticidas/metabolismo Modelos Moleculares [Mh] Termos MeSH secundário: Substituição de Aminoácidos Animais Sítios de Ligação Carbamatos/química Carbamatos/metabolismo Carboxilesterase/química Carboxilesterase/genética Domínio Catalítico Cristalografia por Raios X Proteínas de Insetos/química Proteínas de Insetos/genética Inseticidas/química Cinética Ligantes Conformação Molecular Mutação Organofosfatos/química Organofosfatos/metabolismo Filogenia Conformação Proteica Dobramento de Proteína Proteínas Recombinantes/química Proteínas Recombinantes/metabolismo Especificidade da Espécie Umbeliferonas/química Umbeliferonas/metabolismo [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Carbamates); 0 (Insect Proteins); 0 (Insecticides); 0 (Ligands); 0 (Organophosphates); 0 (Recombinant Proteins); 0 (Umbelliferones); EC 3.1.1.1 (Carboxylesterase) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171024 [Lr] Data última revisão: 171024 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170921 [St] Status: MEDLINE [do] DOI: 10.1021/acs.biochem.7b00774

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[PMID]: 28853883 [Au] Autor: Genovese S; Taddeo VA; Epifano F; Fiorito S; Bize C; Rives A; de Medina P [Ad] Endereço: Department of Pharmacy, University "G. D'Annunzio" of Chieti-Pescara , Via dei Vestini 31, 66100 Chieti Scalo (CH), Italy. [Ti] Título: Characterization of the Degradation Profile of Umbelliprenin, a Bioactive Prenylated Coumarin of a Ferulago Species. [So] Source: J Nat Prod;80(9):2424-2431, 2017 Sep 22. [Is] ISSN: 1520-6025 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Umbelliprenin is a secondary plant metabolite that displays promising chemopreventive, anti-inflammatory, and antigenotoxic properties. It possesses potential for applications to human welfare notably to prevent the emergence of cancer. For this purpose, stability studies are needed to define proper storage conditions and adapted formulations for this drug candidate. The identification of degradative products is a major concern for the preclinical development of umbelliprenin, providing also interesting information related to potential original phytochemicals formed in plants exposed to stressors. The stability profile of umbelliprenin under various stress conditions including exposure to heat, light, oxidation, and hydrolytic medium was assessed via HPLC/UV data. The data support that umbelliprenin undergoes inter- and intramolecular [2+2] cycloaddition under light exposure, leading respectively to a cyclobutane-umbelliprenin dimer and a 16-membered macrocycle. Their structures were characterized via MS and NMR data. It was shown that UV-A filters prevent this process, whereas UV-B filters and antioxidants are not or weakly effective. The study provides useful information for the preclinical development of umbelliprenin as an original chemopreventive agent. [Mh] Termos MeSH primário: Anti-Inflamatórios/química Anti-Inflamatórios/farmacologia Antioxidantes/química Antioxidantes/farmacologia Apiaceae/química Cumarínicos/química Cumarínicos/farmacologia Umbeliferonas/química [Mh] Termos MeSH secundário: Seres Humanos Hidrólise Estrutura Molecular Oxirredução Prenilação [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Inflammatory Agents); 0 (Antioxidants); 0 (Coumarins); 0 (Umbelliferones); A4VZ22K1WT (coumarin); MSD8N8A1LQ (umbelliprenin) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171031 [Lr] Data última revisão: 171031 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170831 [St] Status: MEDLINE [do] DOI: 10.1021/acs.jnatprod.7b00175

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[PMID]: 28850890 [Au] Autor: Han B; Xin Z; Ma S; Liu W; Zhang B; Ran L; Yi L; Ren D [Ad] Endereço: Yunnan Food Safety Research Institute, Kunming University of Science and Technology, Kunming 650500, PR China. [Ti] Título: Comprehensive characterization and identification of antioxidants in Folium Artemisiae Argyi using high-resolution tandem mass spectrometry. [So] Source: J Chromatogr B Analyt Technol Biomed Life Sci;1063:84-92, 2017 Sep 15. [Is] ISSN: 1873-376X [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Antioxidants from natural sources, such as vegetables and fruits, are attracting more and more interest. In this work, we evaluated the antioxidant potential of Folium Artemisia Argyi, a traditional Chinese herb medicine and food supplement. The total phenolic content, total flavonoid content, and antioxidant ability of the crude extracts and fractions obtained from consecutively partition of n-hexane, ethyl acetate, and n-butanol were measured and compared. Ethyl acetate fraction shows the highest total phenolic and flavonoid contents and highest antioxidant capability with regard to DPPH, ABTS, superoxide anion free radical scavenging ability, and ferric-reducing antioxidant power. In addition, the potential antioxidant components were screened by DPPH-UHPLC-MS experiments and subsequently characterized by using high-resolution tandem mass spectrometry. This work finally identified 45 antioxidants, including organic acids, phenolic compounds, flavonoids, and methoxylated flavonoids. The results suggested that Folium Artemisiae Argyi is a potential inexpensive resource of natural antioxidants. [Mh] Termos MeSH primário: Antioxidantes/análise Artemisia/química Medicamentos de Ervas Chinesas/química Espectrometria de Massas em Tandem/métodos [Mh] Termos MeSH secundário: Antioxidantes/química Ácidos Carboxílicos/análise Ácidos Carboxílicos/química Cromatografia Líquida de Alta Pressão/métodos Flavonoides/análise Flavonoides/química Fenóis/análise Fenóis/química Umbeliferonas/análise Umbeliferonas/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antioxidants); 0 (Carboxylic Acids); 0 (Drugs, Chinese Herbal); 0 (Flavonoids); 0 (Phenols); 0 (Umbelliferones); SM2XD6V944 (esculetin) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171002 [Lr] Data última revisão: 171002 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170830 [St] Status: MEDLINE

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[PMID]: 28768532 [Au] Autor: Zingue S; Michel T; Nde CBM; Njuh AN; Cisilotto J; Ndinteh DT; Clyne C; Fernandez X; Creczynski-Pasa TB; Njamen D [Ad] Endereço: Department of Life and Earth Sciences, Higher Teachers' Training College, University of Maroua, P.O. Box 55, Maroua, Cameroon. stephanezingue@gmail.com. [Ti] Título: Estrogen-like and tissue-selective effects of 7-methoxycoumarin from Ficus umbellata (Moraceae): an in vitro and in vivo study. [So] Source: BMC Complement Altern Med;17(1):383, 2017 Aug 02. [Is] ISSN: 1472-6882 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Ficus umbellata is a medicinal plant previously shown to endow estrogenic properties. Its major component was isolated and characterized as 7-methoxycoumarin (MC). Noteworthy, coumarins and the respective active metabolite 7-hydroxycoumarin analogs have shown aromatase inhibitory activity, which is of particular interest in the treatment of estrogen-dependent cancers. The present work aimed at evaluating the estrogenic/antiestrogenic effects of MC in vitro and in vivo. METHODS: To do so, in vitro assays using E-screen and reporter gene were done. In vivo, a 3-day uterotrophic assay followed by a postmenopausal-like rat model to characterize MC as well as F. umbellata aqueous extract in ovariectomized Wistar rats was performed. The investigations focused on histological (vaginal and uterine epithelial height) and morphological (uterine wet weight, vagina stratification and cornification) endpoints, bone mass, biochemical parameters and lipid profile. RESULTS: MC induced a significant (p < 0.05) MCF-7 cell proliferation at a concentration of 0.1 µM, but did not inhibit the effect induced by estradiol in both E-screen and reporter gene assays. In vivo, MC treatment did not show an uterotrophic effect in both rat models used. However, MC (1 mg/kg) induced a significant increase (p < 0.01) of vaginal epithelial height. No significant change was observed with MC in abdominal fat weight, serum lipid levels and bone weight. CONCLUSION: These results suggest that MC has a weak estrogenic activity in vitro and in vivo that accounts only in part to the estrogenicity of the whole plant extract. MC could be beneficial with regard to vagina dryness as it showed a tissue specific effect without exposing the uterus to a potential tumorigenic growth. [Mh] Termos MeSH primário: Estrogênios/metabolismo Ficus/química Fitoestrógenos/farmacologia Extratos Vegetais/farmacologia Umbeliferonas/farmacologia Útero/efeitos dos fármacos Vagina/efeitos dos fármacos [Mh] Termos MeSH secundário: Tecido Adiposo/metabolismo Animais Inibidores da Aromatase/farmacologia Osso e Ossos/efeitos dos fármacos Epitélio/efeitos dos fármacos Estradiol/metabolismo Estradiol/farmacologia Antagonistas de Estrogênios/farmacologia Feminino Células HEK293 Seres Humanos Lipídeos/sangue Células MCF-7 Ovariectomia Pós-Menopausa Ratos Wistar Útero/metabolismo Vagina/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Aromatase Inhibitors); 0 (Estrogen Antagonists); 0 (Estrogens); 0 (Lipids); 0 (Phytoestrogens); 0 (Plant Extracts); 0 (Umbelliferones); 4TI98Z838E (Estradiol) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170901 [Lr] Data última revisão: 170901 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170804 [St] Status: MEDLINE [do] DOI: 10.1186/s12906-017-1895-9

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[PMID]: 28514904 [Au] Autor: Park SH; Sung YY; Nho KJ; Kim DS; Kim HK [Ad] Endereço: * Mibyeong Research Center, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon 305-811, Republic of Korea. [Ti] Título: Effects of Viola mandshurica on Atherosclerosis and Hepatic Steatosis in ApoE[Formula: see text] via the AMPK Pathway. [So] Source: Am J Chin Med;45(4):757-772, 2017. [Is] ISSN: 0192-415X [Cp] País de publicação: Singapore [La] Idioma: eng [Ab] Resumo: Atherosclerosis was previously thought to be a disease that primarily involves lipid accumulation in the arterial wall. In this report, we investigated the effect of Viola mandshurica W. Becker (V. mandshurica) water extract on atherosclerosis in apolipoprotein E deficient (ApoE[Formula: see text]) mice. The administration of V. mandshurica to high-fat diet-fed mice reduced body weight, liver weight, and serum levels of lipids (total cholesterol, low-density lipoprotein-cholesterol, triglycerides), glucose, alanine transaminase, and aspartate transaminase. Histopathologic analyses of the aorta and liver revealed that V. mandshurica attenuated atherosclerotic lesions and reduced lipid accumulation, inflammatory responses and fatty acid synthesis. V. mandshurica also increased phosphorylation of adenosine monophosphate-activated protein kinase (AMPK), thereby reducing acetyl-CoA carboxylase (ACC) in liver tissue and inhibiting sterol regulatory element-binding protein 1c (SREBP-1c). V. mandshurica reduced protein expression levels of adhesion molecules (intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and E-selectin) as well as ACC, fatty acid synthase, and SREBP-1c. In addition, quantitative analysis of V. mandshurica by high-performance liquid chromatography revealed the presence of esculetin and scopoletin. Esculetin and scopoletin reduced adhesion molecules in human aortic smooth muscle cells. Our results indicate that the anti-atherosclerotic effects of V. mandshurica may be associated with activation of the AMPK pathway. Therefore, AMPK-dependent phosphorylation of SREBP-1c by V. mandshurica may be an effective therapeutic strategy for combatting atherosclerosis and hepatic steatosis. [Mh] Termos MeSH primário: Proteínas Quinases Ativadas por AMP/metabolismo Aterosclerose/tratamento farmacológico Aterosclerose/etiologia Fígado Gorduroso/tratamento farmacológico Fígado Gorduroso/etiologia Terapia de Alvo Molecular Fitoterapia Extratos Vegetais/farmacologia Extratos Vegetais/uso terapêutico Viola/química [Mh] Termos MeSH secundário: Acetil-CoA Carboxilase/metabolismo Animais Molécula 1 de Adesão Celular Moléculas de Adesão Celular/metabolismo Modelos Animais de Doenças Imunoglobulinas/metabolismo Metabolismo dos Lipídeos/efeitos dos fármacos Fígado/metabolismo Masculino Camundongos Endogâmicos C57BL Fosforilação/efeitos dos fármacos Extratos Vegetais/química Escopoletina/isolamento & purificação Escopoletina/farmacologia Escopoletina/uso terapêutico Transdução de Sinais Proteína de Ligação a Elemento Regulador de Esterol 1/metabolismo Umbeliferonas/isolamento & purificação Umbeliferonas/farmacologia Umbeliferonas/uso terapêutico [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Cadm1 protein, mouse); 0 (Cell Adhesion Molecule-1); 0 (Cell Adhesion Molecules); 0 (Immunoglobulins); 0 (Plant Extracts); 0 (Srebf1 protein, mouse); 0 (Sterol Regulatory Element Binding Protein 1); 0 (Umbelliferones); EC 2.7.11.31 (AMP-Activated Protein Kinases); EC 6.4.1.2 (Acetyl-CoA Carboxylase); KLF1HS0SXJ (Scopoletin); SM2XD6V944 (esculetin) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170519 [St] Status: MEDLINE [do] DOI: 10.1142/S0192415X17500409

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[PMID]: 28367450 [Au] Autor: Rubio V; García-Pérez AI; Tejedor MC; Herráez A; Diez JC [Ad] Endereço: Unidad de Bioquímica y Biología Molecular, Departamento de Biología de Sistemas, Universidad de Alcalá, Alcalá de Henares, 28871 Madrid, Spain. [Ti] Título: Esculetin Neutralises Cytotoxicity of -BHP but Not of H O on Human Leukaemia NB4 Cells. [So] Source: Biomed Res Int;2017:9491045, 2017. [Is] ISSN: 2314-6141 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The coumarin esculetin shows antioxidant action on some cell types, both by scavenging ROS and by decreasing ROS production. We have previously demonstrated the induction of apoptosis by esculetin on NB4 human leukaemia cells by an ill-defined mechanism related to ROS levels. In this work, we analyze the effect of the simultaneous treatment with esculetin and two oxidants to observe the early events in the mechanism of esculetin-induced apoptosis. Our results show that, from the early time of 15 min, esculetin acts synergistically with H O to decrease cell viability and metabolic activity and to increase apoptosis in NB4 cells. In contrast, the early oxidative effects of -BHP are neutralised by esculetin, protecting human leukaemia NB4 cells from apoptosis. Esculetin seems to restrict the increase in peroxides caused by H O or -BHP in the time interval analyzed. These results contribute to a better understanding of the cytotoxic effect caused by esculetin on NB4 cells. At the same time, the early neutralisation of exogenous oxidants could be of interest to prevent diseases related to oxidative stress imbalance. [Mh] Termos MeSH primário: Antioxidantes/farmacologia Estresse Oxidativo/efeitos dos fármacos Umbeliferonas/administração & dosagem [Mh] Termos MeSH secundário: Apoptose/efeitos dos fármacos Proliferação Celular/efeitos dos fármacos Sobrevivência Celular/efeitos dos fármacos Seres Humanos Peróxido de Hidrogênio/química Peróxido de Hidrogênio/toxicidade Leucemia/tratamento farmacológico Oxirredução Espécies Reativas de Oxigênio/metabolismo Umbeliferonas/química terc-Butil Hidroperóxido/química terc-Butil Hidroperóxido/toxicidade [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antioxidants); 0 (Reactive Oxygen Species); 0 (Umbelliferones); 955VYL842B (tert-Butylhydroperoxide); BBX060AN9V (Hydrogen Peroxide); SM2XD6V944 (esculetin) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170428 [Lr] Data última revisão: 170428 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170404 [St] Status: MEDLINE [do] DOI: 10.1155/2017/9491045

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[PMID]: 28365941 [Au] Autor: Shen JQ; Zhang ZX; Shen CF; Liao JZ [Ad] Endereço: Department of Urology, Huzhou Central Hospital, Zhejiang Province 313000, China. [Ti] Título: Anticarcinogenic effect of Umbelliferone in human prostate carcinoma: An in vitro study. [So] Source: J BUON;22(1):94-101, 2017 Jan-Feb. [Is] ISSN: 1107-0625 [Cp] País de publicação: Greece [La] Idioma: eng [Ab] Resumo: PURPOSE: To explore the chemoprotective effect of umbelliferone (UF) on prostate cancer cell lines, i.e. primary stage (LnCap) and last stage (PC3) prostate cancer together with the effect on the induction of apoptosis and alteration on cell cycle arrest. METHODS: Various concentrations of UF were evaluated against the different prostate cancer cell lines. Lipopolysaccharide (LPS) induced cytokines related factor profiling, proinflammatory cytokines, and inflammatory mediators were studied using Western blot analysis. RESULTS: UF showed significant apoptotic effect. Moreover, treatment with UF did not show apoptosis or cell cycle arrest on the non-cancerous cells including BHP-1, suggesting a selective tumor cell specific effect. UF treatment also enhanced the expression of Bax in PC3 cells, but had no significant effect on the activation of nuclear factor κB (NF-κB). Thus, the apoptosis induction was independent of NF-κB activation. CONCLUSION: The results of the present investigation confirmed the chemoprotective effect of UF in early-stage (Ln- Cap) and late-stage (PC3) prostate cancer cells. [Mh] Termos MeSH primário: Anticarcinógenos/farmacologia Umbeliferonas/farmacologia [Mh] Termos MeSH secundário: Apoptose/efeitos dos fármacos Pontos de Checagem do Ciclo Celular/efeitos dos fármacos Linhagem Celular Tumoral Seres Humanos Masculino NF-kappa B/fisiologia Proteína X Associada a bcl-2/análise [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anticarcinogenic Agents); 0 (BAX protein, human); 0 (NF-kappa B); 0 (Umbelliferones); 0 (bcl-2-Associated X Protein); 60Z60NTL4G (7-hydroxycoumarin) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170831 [Lr] Data última revisão: 170831 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170403 [St] Status: MEDLINE

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