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[PMID]:28453010
[Au] Autor:Sethi S; Ooe M; Sakamoto T; Fujimoto K
[Ad] Endereço:School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan. kenzo@jaist.ac.jp.
[Ti] Título:Effect of nucleobase change on cytosine deamination through DNA photo-cross-linking reaction via 3-cyanovinylcarbazole nucleoside.
[So] Source:Mol Biosyst;13(6):1152-1156, 2017 Jun 01.
[Is] ISSN:1742-2051
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Photo-chemical deamination of cytosine using 3-cyanovinylcarbazole nucleoside ( K) mediated photo-cross-linking is a technique for site-directed mutagenesis. Using this technique in vivo requires the elimination of a high-temperature incubation step; instead, incubation should be carried out under physiological conditions. To improve the reactivity of K mediated photo-cross-link induced deamination of cytosine under physiological conditions, an evaluation of base pairing in cytosine was carried out with respect to its deamination. Guanine was replaced with 4 different counter bases (inosine, 2-aminopurine, 5-nitroindole, and nebularine), showing distinct hydrogen bonding patterns with target cytosine, which was incorporated at the -1 position with respect to K in the K-modified photo-responsive oligodeoxyribonucleotides to ascertain the role of hydrogen bonding in deamination under physiological conditions. Among the counter bases, inosine showed the highest acceleration towards the photo-induced deamination reaction.
[Mh] Termos MeSH primário: Citosina/química
DNA/química
Guanina/química
Nucleosídeos/química
[Mh] Termos MeSH secundário: 2-Aminopurina/química
Pareamento de Bases
Desaminação
Ligações de Hidrogênio
Indóis/química
Estrutura Molecular
Mutagênese Sítio-Dirigida
Oligodesoxirribonucleotídeos/química
Nucleosídeos de Purina/química
Ribonucleosídeos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indoles); 0 (Nucleosides); 0 (Oligodeoxyribonucleotides); 0 (Purine Nucleosides); 0 (Ribonucleosides); 452-06-2 (2-Aminopurine); 5Z93L87A1R (Guanine); 8J337D1HZY (Cytosine); 9007-49-2 (DNA); B8B604PS4P (nebularine); O2BHX6EDBN (5-nitroindole)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1039/c7mb00082k


  2 / 9595 MEDLINE  
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[PMID]:29274339
[Au] Autor:Alniss H; Zamiri B; Khalaj M; Pearson CE; Macgregor RB
[Ad] Endereço:College of Pharmacy, University of Sharjah, P.O. Box 27272, Sharjah, United Arab Emirates.
[Ti] Título:Thermodynamic and spectroscopic investigations of TMPyP4 association with guanine- and cytosine-rich DNA and RNA repeats of C9orf72.
[So] Source:Biochem Biophys Res Commun;495(4):2410-2417, 2018 01 22.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: An expansion of the hexanucleotide repeat (GGGGCC)n·(GGCCCC)n in the C9orf72 promoter has been shown to be the cause of Amyotrophic lateral sclerosis and frontotemporal dementia (ALS-FTD). The C9orf72 repeat can form four-stranded structures; the cationic porphyrin (TMPyP4) binds and distorts these structures. METHODS: Isothermal titration calorimetry (ITC), and circular dichroism (CD) were used to study the binding of TMPyP4 to the C-rich and G-rich DNA and RNA oligos containing the hexanucleotide repeat at pH 7.5 and 0.1 M K . RESULTS: The CD spectra of G-rich DNA and RNA TMPyP4 complexes showed features of antiparallel and parallel G-quadruplexes, respectively. The shoulder at 260 nm in the CD spectrum becomes more intense upon formation of complexes between TMPyP4 and the C-rich DNA. The peak at 290 nm becomes more intense in the c-rich RNA molecules, suggesting induction of an i-motif structure. The ITC data showed that TMPyP4 binds at two independent sites for all DNA and RNA molecules. CONCLUSIONS: For DNA, the data are consistent with TMPyP4 stacking on the terminal tetrads and intercalation. For RNA, the thermodynamics of the two binding modes are consistent with groove binding and intercalation. In both cases, intercalation is the weaker binding mode. These findings are considered with respect to the structural differences of the folded DNA and RNA molecules and the energetics of the processes that drive site-specific recognition by TMPyP4; these data will be helpful in efforts to optimize the specificity and affinity of the binding of porphyrin-like molecules.
[Mh] Termos MeSH primário: Proteína C9orf72/química
Proteína C9orf72/genética
Citosina/química
DNA/química
Guanina/química
RNA/química
Sequências Repetitivas de Ácido Nucleico
[Mh] Termos MeSH secundário: Composição de Bases
Sítios de Ligação
Calorimetria
Dicroísmo Circular
DNA/genética
Ligação Proteica
RNA/genética
Relação Estrutura-Atividade
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (C9orf72 Protein); 5Z93L87A1R (Guanine); 63231-63-0 (RNA); 8J337D1HZY (Cytosine); 9007-49-2 (DNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171224
[St] Status:MEDLINE


  3 / 9595 MEDLINE  
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[PMID]:29242886
[Au] Autor:Romero EE; Hernandez FE
[Ad] Endereço:Department of Chemistry, University of Central Florida, P. O. Box 162366, Orlando, Florida 32816-2366, USA. florencio.hernandez@ucf.edu.
[Ti] Título:Solvent effect on the intermolecular proton transfer of the Watson and Crick guanine-cytosine and adenine-thymine base pairs: a polarizable continuum model study.
[So] Source:Phys Chem Chem Phys;20(2):1198-1209, 2018 Jan 03.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Herein we present our results on the study of the double proton transfer (DPT) mechanism in the adenine-thymine (AT) and guanine-cytosine (GC) base pairs, both in gas phase and in solution. The latter was modeled using the polarizable continuum method (PCM) in different solvents. According to our DFT calculations, the DPT may occur for both complexes in a stepwise mechanism in condensate phase. In gas phase only the GC base pair exhibits a concerted DPT mechanism. Using the Wigner's tunneling corrections to the transition state theory we demonstrate that such corrections are important for the prediction of the rate constants of both systems in gas and in condensate phase. We also show that (i) as the polarity of the medium decreases the equilibrium constant of the DPT reaction increases in both complexes, and (ii) that the equilibrium constant in the GC complex is four orders of magnitude larger than in AT. This observation suggests that the spontaneous mutations in DNA base pairs are more probable in GC than in AT.
[Mh] Termos MeSH primário: Pareamento de Bases
DNA/química
Modelos Moleculares
[Mh] Termos MeSH secundário: Adenina
Citosina
Guanina
Prótons
Solventes
Timina
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Protons); 0 (Solvents); 5Z93L87A1R (Guanine); 8J337D1HZY (Cytosine); 9007-49-2 (DNA); JAC85A2161 (Adenine); QR26YLT7LT (Thymine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1039/c7cp05356h


  4 / 9595 MEDLINE  
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[PMID]:29230450
[Au] Autor:Ren W; Zheng K; Liao C; Yang J; Zhao J
[Ad] Endereço:Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. jzhao@iccas.ac.cn.
[Ti] Título:Charge evolution during the unfolding of a single DNA i-motif.
[So] Source:Phys Chem Chem Phys;20(2):916-924, 2018 Jan 03.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The effective charge and evolution of single chains of a DNA i-motif during its unfolding process are investigated at the single molecule level. Using fluorescence correlation spectroscopy and photon counting histograms, the single chain dimensions and electrical potential of cytosine-rich human telomeric oligonucleotides are monitored, during their unfolding from the i-motif to the random coil state. It is discovered that the effective charge density of the DNA chain is very sensitive to conformation changes and the results remarkably expose the existence of an intermediate state of the unfolding process. A huge difference in pH value exists in the vicinity of the DNA chain and the bulk solution, depending on the salt concentration, as reflected by a down-shift in the pH value of unfolding. The presence of an external salt in the solution helps to stabilize the i-motif structure at low pH values due to the reduction of the effective charge density. It can also destabilize the folded structure in the pH range of the conformation transition due to the elevation of the local pH value, encouraging the deprotonation of the cytosine groups. These results provide new information for understanding the structure and stability of i-motif DNA, and its biological function, as well as the building blocks for smart nanomaterials.
[Mh] Termos MeSH primário: DNA/química
Conformação de Ácido Nucleico
Motivos de Nucleotídeos
Dobramento de Proteína
Telômero/química
[Mh] Termos MeSH secundário: Citosina/química
Oligonucleotídeos
Desnaturação Proteica
Espectrometria de Fluorescência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oligonucleotides); 8J337D1HZY (Cytosine); 9007-49-2 (DNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.1039/c7cp06235d


  5 / 9595 MEDLINE  
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[PMID]:29320657
[Au] Autor:Hibbert KA; Shepard JO; Lane RJ; Azar MM
[Ad] Endereço:From the Departments of Medicine (K.A.H., R.J.L.), Radiology (J.-A.O.S.), and Pathology (M.M.A.), Massachusetts General Hospital, and the Departments of Medicine (K.A.H., R.J.L.), Radiology (J.-A.O.S.), and Pathology (M.M.A.), Harvard Medical School - both in Boston.
[Ti] Título:Case 1-2018. A 39-Year-Old Woman with Rapidly Progressive Respiratory Failure.
[So] Source:N Engl J Med;378(2):182-190, 2018 01 11.
[Is] ISSN:1533-4406
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Infecções por Adenoviridae/diagnóstico
Adenoviridae/isolamento & purificação
Pulmão/patologia
Infecções Oportunistas/diagnóstico
Insuficiência Respiratória/etiologia
[Mh] Termos MeSH secundário: Infecções por Adenoviridae/tratamento farmacológico
Adulto
Antivirais/efeitos adversos
Antivirais/uso terapêutico
Citosina/efeitos adversos
Citosina/análogos & derivados
Citosina/uso terapêutico
Diagnóstico Diferencial
Feminino
Seres Humanos
Hospedeiro Imunocomprometido
Leucemia Mieloide Aguda/complicações
Leucemia Mieloide Aguda/terapia
Pulmão/diagnóstico por imagem
Nasofaringe/virologia
Infecções Oportunistas/tratamento farmacológico
Organofosfonatos/efeitos adversos
Organofosfonatos/uso terapêutico
Radiografia Torácica
Insuficiência Renal/induzido quimicamente
Transplante de Células-Tronco/efeitos adversos
Tomografia Computadorizada por Raios X
Carga Viral
[Pt] Tipo de publicação:CASE REPORTS; CLINICAL CONFERENCE; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Organophosphonates); 8J337D1HZY (Cytosine); JIL713Q00N (cidofovir)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180118
[Lr] Data última revisão:
180118
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE
[do] DOI:10.1056/NEJMcpc1712222


  6 / 9595 MEDLINE  
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[PMID]:27770361
[Au] Autor:Guevara MÁ; de María N; Sáez-Laguna E; Vélez MD; Cervera MT; Cabezas JA
[Ad] Endereço:Department of Forest Ecology and Genetic, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria - Centro de InvestigaciónForestal (INIA-CIFOR), Ctra. de La Coruña Km 7,5, Madrid, 28040, Spain.
[Ti] Título:Analysis of DNA Cytosine Methylation Patterns Using Methylation-Sensitive Amplification Polymorphism (MSAP).
[So] Source:Methods Mol Biol;1456:99-112, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Different molecular techniques have been developed to study either the global level of methylated cytosines or methylation at specific gene sequences. One of them is the methylation-sensitive amplified polymorphism technique (MSAP) which is a modification of amplified fragment length polymorphism (AFLP). It has been used to study methylation of anonymous CCGG sequences in different fungi, plants, and animal species. The main variation of this technique resides on the use of isoschizomers with different methylation sensitivity (such as HpaII and MspI) as a frequent-cutter restriction enzyme. For each sample, MSAP analysis is performed using both EcoRI/HpaII- and EcoRI/MspI-digested samples. A comparative analysis between EcoRI/HpaII and EcoRI/MspI fragment patterns allows the identification of two types of polymorphisms: (1) methylation-insensitive polymorphisms that show common EcoRI/HpaII and EcoRI/MspI patterns but are detected as polymorphic amplified fragments among samples and (2) methylation-sensitive polymorphisms which are associated with the amplified fragments that differ in their presence or absence or in their intensity between EcoRI/HpaII and EcoRI/MspI patterns. This chapter describes a detailed protocol of this technique and discusses the modifications that can be applied to adjust the technology to different species of interest.
[Mh] Termos MeSH primário: Análise do Polimorfismo de Comprimento de Fragmentos Amplificados
Citosina/metabolismo
Metilação de DNA
Epigênese Genética
Epigenômica/métodos
[Mh] Termos MeSH secundário: Ilhas de CpG
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
8J337D1HZY (Cytosine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180112
[Lr] Data última revisão:
180112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE


  7 / 9595 MEDLINE  
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[PMID]:27770358
[Au] Autor:Bilichak A; Kovalchuk I
[Ad] Endereço:Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, AB, Canada. a.bilichak@gmail.com.
[Ti] Título:Analysis of Global Genome Methylation Using the Cytosine-Extension Assay.
[So] Source:Methods Mol Biol;1456:73-79, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:DNA methylation is a reversible covalent chemical modification of DNA intended to regulate chromatin structure and gene expression in a cell- and tissue-specific manner and in response to the environment. Cytosine methylation is predominantly occurring in plants, and cytosine nucleotides in plants can be methylated at symmetrical (CpG and CpHpG) and nonsymmetrical sites. Although there exists a number of various methods for the detection of cytosine methylation, most of them are either laborious or expensive or both. Here, we describe a quick inexpensive method for the analysis of global genome methylation using a cytosine-extension assay. The assay can be used for the analysis of the total level of CpG, CpHpG, and CpHpH methylation in a given sample of plant DNA.
[Mh] Termos MeSH primário: Citosina/metabolismo
Metilação de DNA
Epigenômica/métodos
Genoma
[Mh] Termos MeSH secundário: Ilhas de CpG
Enzimas de Restrição do DNA/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
8J337D1HZY (Cytosine); EC 3.1.21.- (DNA Restriction Enzymes)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180112
[Lr] Data última revisão:
180112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE


  8 / 9595 MEDLINE  
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[PMID]:29190698
[Au] Autor:Foksinski M; Zarakowska E; Gackowski D; Skonieczna M; Gajda K; Hudy D; Szpila A; Bialkowski K; Starczak M; Labejszo A; Czyz J; Rzeszowska-Wolny J; Olinski R
[Ad] Endereço:Department of Clinical Biochemistry, Faculty of Pharmacy, L. Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Bydgoszcz, Poland.
[Ti] Título:Profiles of a broad spectrum of epigenetic DNA modifications in normal and malignant human cell lines: Proliferation rate is not the major factor responsible for the 5-hydroxymethyl-2'-deoxycytidine level in cultured cancerous cell lines.
[So] Source:PLoS One;12(11):e0188856, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Active demethylation of 5-methylcytosine moiety in DNA occurs by its sequential oxidation to 5-hydroxymethylcytosine, 5-formylcytosine and 5-carboxycytosine, catalysed by enzymes of the Ten-Eleven Translocation family proteins (TETs 1, 2 and 3). Here we analyzed for the first time all the intermediate products of DNA demethylation pathway in the form of deoxynucleosides (5-methyl-2'-deoxycytidine, 5-(hydroxymethyl)-2'-deoxycytidine, 5-formyl-2'-deoxycytidine and 5-carboxy-2'-deoxycytidine as well as 5-(hydroxymethyl)-2'-deoxyuridine) using automated isotope-dilution online two-dimensional ultra-performance liquid chromatography with tandem mass spectrometry. DNA was isolated from human malignant cell lines of colon adenocarcinoma (HCT 116), melanoma (Me45), myelogenous leukemia bone marrow blasts (K562), EBV-positive Burkitt's lymphoma lymphoblasts (Raji), EBV-negative Burkitt's lymphoma lymphoblasts (male-CA46 and female-ST486), as well as normal neonatal dermal fibroblasts (NHDF-Neo). The expression levels of TET1, TET2, TET3, SMUG1, and TDG genes were also assayed by RT-qPCR. Our results show a global erasure of 5-hydroxymethyl-2'-deoxycytidine and 5-carboxy-2'-deoxycytidine in DNA of cultured cells compared with DNA from primary malignant tissue. Moreover, malignant cells in culture have a quite different DNA epigenetic profile than cultured normal cells, and different types of malignant cells display different and characteristic profiles of DNA epigenetic marks. Similar analyses of a broader spectrum of epigenetic modifications, not restricted to 5-methyl-2'-deoxycytidine, could lead to better understanding of the mechanism(s) responsible for emergence of different types of cancer cells.
[Mh] Termos MeSH primário: Proliferação Celular/efeitos dos fármacos
DNA/genética
Desoxicitidina/análogos & derivados
Epigênese Genética
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Cromatografia Líquida
Citosina/análise
DNA/química
Desoxicitidina/farmacologia
Seres Humanos
Reação em Cadeia da Polimerase em Tempo Real
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Espectrometria de Massas em Tandem
Timina/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (5-hydroxymethyl-2'-deoxycytidine); 0W860991D6 (Deoxycytidine); 8J337D1HZY (Cytosine); 9007-49-2 (DNA); QR26YLT7LT (Thymine)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188856


  9 / 9595 MEDLINE  
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[PMID]:29176672
[Au] Autor:Iwan K; Rahimoff R; Kirchner A; Spada F; Schröder AS; Kosmatchev O; Ferizaj S; Steinbacher J; Parsa E; Müller M; Carell T
[Ad] Endereço:Center for Integrated Protein Science Munich CiPSM at the Department of Chemistry, Ludwig-Maximilians-Universität München, Munich, Germany.
[Ti] Título:5-Formylcytosine to cytosine conversion by C-C bond cleavage in vivo.
[So] Source:Nat Chem Biol;14(1):72-78, 2018 Jan.
[Is] ISSN:1552-4469
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tet enzymes oxidize 5-methyl-deoxycytidine (mdC) to 5-hydroxymethyl-dC (hmdC), 5-formyl-dC (fdC) and 5-carboxy-dC (cadC) in DNA. It was proposed that fdC and cadC deformylate and decarboxylate, respectively, to dC over the course of an active demethylation process. This would re-install canonical dC bases at previously methylated sites. However, whether such direct C-C bond cleavage reactions at fdC and cadC occur in vivo remains an unanswered question. Here we report the incorporation of synthetic isotope- and (R)-2'-fluorine-labeled dC and fdC derivatives into the genome of cultured mammalian cells. Following the fate of these probe molecules using UHPLC-MS/MS provided quantitative data about the formed reaction products. The data show that the labeled fdC probe is efficiently converted into the corresponding labeled dC, most likely after its incorporation into the genome. Therefore, we conclude that fdC undergoes C-C bond cleavage in stem cells, leading to the direct re-installation of unmodified dC.
[Mh] Termos MeSH primário: Citosina/análogos & derivados
DNA/metabolismo
Desoxicitidina/metabolismo
[Mh] Termos MeSH secundário: Animais
Isótopos de Carbono
Linhagem Celular
Cromatografia Líquida de Alta Pressão
Citosina/química
Citosina/metabolismo
DNA/química
DNA (Citosina-5-)-Metiltransferase 1/metabolismo
Desmetilação
Desoxicitidina/química
Metilação
Camundongos
Isótopos de Nitrogênio
Oxirredução
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (5-formylcytosine); 0 (Carbon Isotopes); 0 (Nitrogen Isotopes); 0W860991D6 (Deoxycytidine); 8J337D1HZY (Cytosine); 9007-49-2 (DNA); EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferase 1)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171225
[Lr] Data última revisão:
171225
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1038/nchembio.2531


  10 / 9595 MEDLINE  
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[PMID]:28741224
[Au] Autor:Paul A; Dasgupta P; Roy D; Chaudhuri S
[Ad] Endereço:Division of Plant Biology, Bose Institute, P 1/12 C.I.T. Scheme VII M, Kolkata, 700054, India.
[Ti] Título:Comparative analysis of Histone modifications and DNA methylation at OsBZ8 locus under salinity stress in IR64 and Nonabokra rice varieties.
[So] Source:Plant Mol Biol;95(1-2):63-88, 2017 Sep.
[Is] ISSN:1573-5028
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Rice being an important cereal crop is highly sensitive to salinity stress causing growth retardation and loss in productivity. However, certain rice genotypes like Nonabokra and Pokkali show a high level of tolerance towards salinity stress compared to IR64 variety. This differential response of tolerant varieties towards salinity stress may be a cumulative effect of genetic and epigenetic factors. In this study, we have compared the salinity-induced changes in chromatin modifications at the OsBZ8 locus in salt-tolerant Nonabokra and salt-sensitive IR64 rice varieties. Expression analysis indicates that the OsBZ8 gene is highly induced in Nonabokra plants even in the absence of salt stress, whereas in IR64, the expression significantly increases only during salt stress. Sequence analysis and nucleosomal arrangement within the region -2000 to +1000 of OsBZ8 gene show no difference between the two rice varieties. However, there was a considerable difference in histone modifications and DNA methylation at the locus between these varieties. In Nonabokra, the upstream region was hyperacetylated at H3K9 and H3K27, and this acetylation did not change during salt stress. However, in IR64, histone acetylation was observed only during salt stress. Moreover, the upstream region of OsBZ8 gene has highly dynamic nucleosome arrangement in Nonabokra, compared to IR64. Furthermore, loss of DNA methylation was observed at OsBZ8 locus in Nonabokra control plants along with low H3K27me3 and high H3K4me3. Control IR64 plants show high DNA methylation and enriched H3K27me3. Collectively these results indicate a significant difference in chromatin modifications between the rice varieties that regulates differential expression of OsBZ8 gene during salt stress.
[Mh] Termos MeSH primário: Metilação de DNA/genética
Loci Gênicos
Histonas/metabolismo
Oryza/genética
Oryza/fisiologia
Processamento de Proteína Pós-Traducional
Salinidade
Estresse Fisiológico/efeitos dos fármacos
[Mh] Termos MeSH secundário: Acetilação/efeitos dos fármacos
Citosina/metabolismo
Metilação de DNA/efeitos dos fármacos
Inibidores Enzimáticos/farmacologia
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Genes de Plantas
Lisina/metabolismo
Modelos Biológicos
Nucleossomos/efeitos dos fármacos
Nucleossomos/metabolismo
Oryza/efeitos dos fármacos
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Regiões Promotoras Genéticas/genética
Processamento de Proteína Pós-Traducional/efeitos dos fármacos
Tolerância a Sal/efeitos dos fármacos
Tolerância a Sal/genética
Cloreto de Sódio/farmacologia
Estresse Fisiológico/genética
Transcrição Genética/efeitos dos fármacos
Regulação para Cima/efeitos dos fármacos
Regulação para Cima/genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzyme Inhibitors); 0 (Histones); 0 (Nucleosomes); 0 (Plant Proteins); 451W47IQ8X (Sodium Chloride); 8J337D1HZY (Cytosine); K3Z4F929H6 (Lysine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1007/s11103-017-0636-2



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