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  1 / 917 MEDLINE  
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[PMID]:28351621
[Au] Autor:Tochigi Y; Iwasaki Y; Sano M; Yasuda H; Katayama K; Suzuki H
[Ad] Endereço:Laboratory of Veterinary Physiology, Department of Basic Veterinary Medicine, Division of Functional Morphology, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan.
[Ti] Título:Critical roles of Astrin in the mitosis of immature rat Sertoli cells.
[So] Source:Biochem Biophys Res Commun;486(4):958-964, 2017 May 13.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Male hypogonadism (hgn/hgn) rats show testicular hypoplasia accompanied by dysplastic development of seminiferous tubules due to loss-of-function mutation of the gene encoding Astrin, which is required for mitotic progression in the division cycle of HeLa cells. In the present study, we examined the cytological base leading to the decrease of Sertoli cells in hgn/hgn testes. In hgn/hgn testes on postnatal day 3, anti-phospho-histone H3 (Ser10) (pH3)-positive mitotic phase and TUNEL-positive apoptosis increased in GATA4-positive Sertoli cells. Isolated immature Sertoli cells from hgn/hgn testes showed increased pH3-assessed mitotic index accompanied by decreased 5-bromo-2'-deoxyuridine-incorporation and increased TUNEL-positive apoptosis, suggesting mitotic delay and cell death. In the visualization of mitotic progression by nocodazole (NOC)-mediated cell cycle arrest and subsequent release, hgn/hgn rat-derived Sertoli cells failed to make the transition from prometaphase to metaphase, and the cells with micronuclei and TUNEL-positive cells gradually increased in a time-dependent manner. Western blot analysis detected ≈142 kDa protein expected as Astrin in extracts of +/+ and +/hgn testes and cultured normal Sertoli cells but not in extracts of hgn/hgn testes. CLASP1 was detected in extracts of both normal and hgn/hgn testes, whereas it was localized in kinetochore of normal mitotic Sertoli cells but diffused in cytoplasm of hgn/hgn Sertoli cells. These results indicate that Astrin is required for normal mitotic progression in immature Sertoli cells and that the most severe type of testicullar dysplasia in hgn/hgn rats is caused by mitotic cell death of immature Sertoli cells due to lack of Astrin.
[Mh] Termos MeSH primário: Azul Alciano/metabolismo
Apoptose/fisiologia
Cinetocoros/metabolismo
Proteínas Associadas aos Microtúbulos/metabolismo
Mitose/fisiologia
Fenazinas/metabolismo
Fenotiazinas/metabolismo
Resorcinóis/metabolismo
Células de Sertoli/fisiologia
[Mh] Termos MeSH secundário: Animais
Núcleo Celular/metabolismo
Células Cultivadas
Masculino
Camundongos Endogâmicos
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CLASP1 protein, rat); 0 (Microtubule-Associated Proteins); 0 (Phenazines); 0 (Phenothiazines); 0 (Resorcinols); 83097-09-0 (astrin); P4448TJR7J (Alcian Blue)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170619
[Lr] Data última revisão:
170619
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170330
[St] Status:MEDLINE


  2 / 917 MEDLINE  
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[PMID]:28254103
[Au] Autor:Zhang W; Wang Z; Jia S; Tian Y; Wang G; Li H; Fuxe K
[Ad] Endereço:Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.
[Ti] Título:Is There Volume Transmission Along Extracellular Fluid Pathways Corresponding to the Acupuncture Meridians?
[So] Source:J Acupunct Meridian Stud;10(1):5-19, 2017 Jan.
[Is] ISSN:2093-8152
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Volume transmission is a new major communication signaling via extracellular fluid (interstitial fluid) pathways. It was proposed by the current authors that such pathways can explain the meridian phenomena and acupuncture effects. To investigate whether meridian-like structures exist in fish body and operate via volume transmission in extracellular fluid pathways, we injected alcian blue (AB) under anesthesia into Gephyrocharax melanocheir, which has a translucent body. The migration of AB could be seen directly and was recorded by a digital camera. The fish was then embedded and cut transversely to observe the position of tracks in three dimensions. Eight longitudinal threadlike blue tracks were recognized on the fish. The positions of these threadlike tracks were similar to meridians on the human body. Transverse sections showed that these tracks distributed to different layers of distinct subcutaneous loose connective tissues and intermuscular septa. Lymphatic vessels were sometimes associated with the extracellular blue tracks where the migration of AB occurred. Extracellular fluid pathways were found on fish through their transport of AB. These pathways operating via volume transmission appeared to be similar in positions and functions to the acupuncture meridians in Chinese medicine.
[Mh] Termos MeSH primário: Characidae/fisiologia
Vasos Linfáticos/fisiologia
Meridianos
[Mh] Termos MeSH secundário: Azul Alciano/química
Azul Alciano/farmacocinética
Animais
Seres Humanos
Medicina Tradicional Chinesa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
P4448TJR7J (Alcian Blue)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170313
[Lr] Data última revisão:
170313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE


  3 / 917 MEDLINE  
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[PMID]:27647932
[Au] Autor:Osho SO; Wang T; Horn NL; Adeola O
[Ti] Título:Comparison of goblet cell staining methods in jejunal mucosa of turkey poults.
[So] Source:Poult Sci;96(3):556-559, 2017 Mar 01.
[Is] ISSN:1525-3171
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study compared the intestinal goblet cell density of turkey poults at 2 different ages using Alcian blue-periodic acid-Shiff (AB-PAS) and mucicarmine stains. Neutral mucins are stained with periodic acid-Shiff whereas acidic mucins are stained with Alcian blue. Mucicarmine and AB-PAS are specific to the mucins of epithelial origin. Mucicarmine has only been used for the assessment of goblet cells in human specimens, and it may have advantages for use in animals as a result of the methodological simplicity of staining as compared to AB-PAS. A mid-section of jejunum was taken from 80 turkey poults at 21 and 28 d, and fixed in 10% buffered formalin for 48 h. Each fixed tissue was dehydrated with ethanol, cleared with Sub-X, placed in paraffin wax, prepared on 2 slides, cleared and hydrated. The 2 slides were randomly assigned to 2 treatments which consisted of AB-PAS and mucicarmine stains in a completely randomized design. Goblet cell counts were taken from four villi per slide and the villus height was measured and averaged. There was no difference in the goblet cell density between the staining methods AB-PAS and mucicarmine at 21 or 28 d posthatch. These results show that both staining methods are viable for assessment of goblet cell density in turkey poults.
[Mh] Termos MeSH primário: Células Caliciformes/citologia
Jejuno/citologia
Coloração e Rotulagem/veterinária
Perus
[Mh] Termos MeSH secundário: Azul Alciano/química
Animais
Carmim/química
Reação do Ácido Periódico de Schiff/veterinária
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
51395-97-2 (mucicarmine); CID8Z8N95N (Carmine); P4448TJR7J (Alcian Blue)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160921
[St] Status:MEDLINE
[do] DOI:10.3382/ps/pew324


  4 / 917 MEDLINE  
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[PMID]:27526158
[Au] Autor:Kim J; Kim DH; Jung SJ; Soh KS
[Ad] Endereço:Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon, 443-270, South Korea.
[Ti] Título:Temporal Change of Alcian Blue-Stained Primo Vascular System in Lymph Vessels of Rats.
[So] Source:Adv Exp Med Biol;923:311-317, 2016.
[Is] ISSN:0065-2598
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study aims to investigate the temporal change of a vascular system now known as the primo vascular system (PVS). We used Alcian blue (AB) dye for imaging the distribution of the PVS in lymphatic vessels. The target lymph vessels were chosen as they are easily accessible from the skin, and long-term observation is possible with intact physiological conditions due to a minimal surgical procedure. AB solution was injected into the inguinal lymph node and the target lymph vessels were located along the superficial epigastric vessels. The imaging system allowed processing for extraction of images showing changes in the AB intensity of the visualized PVS components. This newly developed procedure can be used for further study on various dynamic processes of PVS in lymph vessels.
[Mh] Termos MeSH primário: Terapia por Acupuntura/métodos
Azul Alciano/administração & dosagem
Corantes/administração & dosagem
Vasos Linfáticos/anatomia & histologia
Meridianos
Coloração e Rotulagem/métodos
[Mh] Termos MeSH secundário: Animais
Injeções
Masculino
Ratos Sprague-Dawley
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Coloring Agents); P4448TJR7J (Alcian Blue)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160816
[St] Status:MEDLINE
[do] DOI:10.1007/978-3-319-38810-6_41


  5 / 917 MEDLINE  
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[PMID]:27294640
[Au] Autor:Tomaszewski M; Olchowik G; Tomaszewska M; Dworzanski W; Burdan F
[Ad] Endereço:Department of Human Anatomy, Medical University, Lublin, Poland.
[Ti] Título:The influence of caffeine administered at 10°C on bone tissue development.
[So] Source:Ann Agric Environ Med;23(2):319-23, 2016 Jun 02.
[Is] ISSN:1898-2263
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION AND OBJECTIVE: Caffeine is a natural methylxanthine widespread throughout the food industry. Many research studies have shown that caffeine readily crosses the placenta causing teratogenic and embryotoxic effects. The objective of this study was to assess the influence of caffeine, administered at 10°C, on the development of a rat's bone tissue, with particular reference to elemental bone composition using an X-ray microprobe. MATERIALS AND METHODS: The research was conducted on white rats of the Wistar strain. The fertilized females were divided into two groups: an Experimental Group (Group E) and a Control Group (Group C). The females in Group E were given caffeine orally (at 10°C) in 30 mg/day doses from the 8(th) - 21(st) day of pregnancy. The females in Group C were given water at the same temperature. The foetuses were used to assess the growth and mineralization of the skeleton. Qualitative analysis of the morphology and mineralization of bones was conducted using the alcian-alizarin method. For calcium and potassium analysis, an X-ray microprobe was used. RESULTS: By staining the skeleton using the alcian-alizarin method, changes in 47 Group E foetuses were observed. The frequency of the development variants in the Group E rats was statistically higher, compared with Group C. CONCLUSIONS: On the basis of these results, it can be concluded that caffeine in high doses disturbs the development of bone tissue. An additional factor which enhances the adverse effects of this substance on bone tissue is the temperature of the administered solution (10(o)C). In the Experimental Group, a significant decrease in the calcium level, as well as an increase in the potassium level, was observed. The X-ray microprobe can be a perfect complement to the methods which enable determination of the mineralization of osseous tissue.
[Mh] Termos MeSH primário: Desenvolvimento Ósseo/efeitos dos fármacos
Cafeína/farmacologia
[Mh] Termos MeSH secundário: Azul Alciano
Animais
Antraquinonas
Osso e Ossos/diagnóstico por imagem
Osso e Ossos/efeitos dos fármacos
Osso e Ossos/ultraestrutura
Cafeína/administração & dosagem
Cálcio/metabolismo
Feminino
Microscopia Eletrônica de Varredura
Potássio/metabolismo
Ratos
Ratos Wistar
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthraquinones); 3G6A5W338E (Caffeine); 60MEW57T9G (alizarin); P4448TJR7J (Alcian Blue); RWP5GA015D (Potassium); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170113
[Lr] Data última revisão:
170113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160614
[St] Status:MEDLINE
[do] DOI:10.5604/12321966.1203898


  6 / 917 MEDLINE  
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[PMID]:27126824
[Au] Autor:Vázquez-Vélez GE; Rodríguez-Molina JF; Quiñones-Frías MC; Pagán M; García-Arrarás JE
[Ad] Endereço:Program in Developmental Biology and Medical Scientist Training Program, Baylor College of Medicine, Houston, Texas (GEV-V)
[Ti] Título:A Proteoglycan-Like Molecule Offers Insights Into Ground Substance Changes During Holothurian Intestinal Regeneration.
[So] Source:J Histochem Cytochem;64(6):381-93, 2016 06.
[Is] ISSN:1551-5044
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Extracellular matrix remodeling is an essential component of regenerative processes in metazoans. Among these animals, holothurians (sea cucumbers) are distinguished by their great regenerative capacities. We have previously shown that fibrous collagen as well as other fibrous components disappear from the connective tissue (CT) early during intestinal regeneration, and later return as the organ primordia form. We now report on changes of the nonfibrous component of the CT. We have used Alcian Blue staining and an antibody, Proteoglycan Like-1 (PGL-1), that recognizes a proteoglycan-like antigen to identify the presence of proteoglycans in normal and regenerating intestines. Our results show that early in regeneration, the ground substance resembles that of the mesentery, the structure from where the new intestine originates. As regeneration proceeds, Alcian Blue staining and PGL-1 labeling reorganize, so that by 4 weeks the normal intestinal CT pattern is achieved. Together with our previous findings, the data suggest that CT components that might be detrimental to regeneration disappear early on, while those that might be beneficial to regeneration, such as proteoglycans, are present throughout the regenerative process.
[Mh] Termos MeSH primário: Proteoglicanas/metabolismo
Pepinos-do-Mar/fisiologia
[Mh] Termos MeSH secundário: Azul Alciano
Animais
Corantes
Tecido Conjuntivo/metabolismo
Matriz Extracelular/metabolismo
Intestinos/fisiologia
Mesentério/metabolismo
Regeneração
Coloração e Rotulagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coloring Agents); 0 (Proteoglycans); P4448TJR7J (Alcian Blue)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170904
[Lr] Data última revisão:
170904
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160430
[St] Status:MEDLINE
[do] DOI:10.1369/0022155416645781


  7 / 917 MEDLINE  
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[PMID]:26937963
[Au] Autor:Jang H; Yoon J; Gil H; Jung SJ; Kim MS; Lee JK; Kim YJ; Soh KS
[Ad] Endereço:Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon, 443-270, Korea.
[Ti] Título:Observation of a Flowing Duct in the Abdominal Wall by Using Nanoparticles.
[So] Source:PLoS One;11(3):e0150423, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The primo vascular system (PVS) is being established as a circulatory system that corresponds to acupuncture meridians. There have been two critical questions in making the PVS accepted as a novel liquid flowing system. The first one was directly to show the flow of liquid in PVS and the second one was to explain why it was not observed in the conventional histological study of animal tissues. Flow in the PVS in the abdominal cavity was previously verified by injecting Alcian blue into a primo node. However, the tracing of the dye to other subsystems of the PVS has not been done. In the current work we injected fluorescent nanoparticles (FNPs) into a primo node and traced them along a primo vessel which was inside a fat tissue in the abdominal wall. Linea alba is a white middle line in the abdominal skin of a mammal and a band of fat tissue is located in parallel to the linea alba in the parietal side of the abdominal wall of a rat. In this fat band a primo vessel runs parallel to the prominent blood vessels in the fat band and is located just inside the parietal peritoneum. About the second question on the reason why the PVS was not in conventional histological study the current work provided the answer. Histological analysis with hematoxyline and eosine, Masson's trichrome, and Toluidine blue could not discriminate the primo vessel even when we knew the location of the PVS by the trace of the FNPs. This clearly explains why the PVS is hard to observe in conventional histology: it is not a matter of resolution but the contrast. The PVS has very similar structure to the connective tissues that surround the PVS. In the current work we propose a method to find the PVS: Observation of mast cell distribution with toluidine blue staining and the PN has a high density of mast cells, while the lymph node has low density.
[Mh] Termos MeSH primário: Cavidade Abdominal/anatomia & histologia
Gordura Abdominal/anatomia & histologia
Parede Abdominal/anatomia & histologia
Pontos de Acupuntura
Nanopartículas/química
Coloração e Rotulagem/métodos
[Mh] Termos MeSH secundário: Cavidade Abdominal/irrigação sanguínea
Gordura Abdominal/irrigação sanguínea
Gordura Abdominal/citologia
Parede Abdominal/irrigação sanguínea
Azul Alciano/química
Animais
Corantes/química
Amarelo de Eosina-(YS)
Hematoxilina
Seres Humanos
Linfonodos/irrigação sanguínea
Linfonodos/citologia
Vasos Linfáticos/anatomia & histologia
Vasos Linfáticos/irrigação sanguínea
Masculino
Mastócitos/citologia
Ratos
Ratos Sprague-Dawley
Reologia
Rodaminas/química
Cloreto de Tolônio/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Coloring Agents); 0 (Rhodamines); 15XUH0X66N (Tolonium Chloride); K7G5SCF8IL (rhodamine B); P4448TJR7J (Alcian Blue); TDQ283MPCW (Eosine Yellowish-(YS)); YKM8PY2Z55 (Hematoxylin)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160304
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0150423


  8 / 917 MEDLINE  
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[PMID]:26860566
[Au] Autor:Yu F; Guo M; Deng Y; Lu Y; Chen L; Huang P; Li D
[Ad] Endereço:Cancer Research Center, Medical College, Xiamen University.
[Ti] Título:Structure-matched Phthalocyanine Ion Pair as a Red-emitting Fluorescent Optical Probe for the Analysis of Sodium Dodecylbenzenesulfonate with High Specificity and Sensitivity.
[So] Source:Anal Sci;32(2):201-5, 2016.
[Is] ISSN:1348-2246
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:We have found that a positively charged cationic copper phthalocyanine, Alcian blue (Alcian blue 8GX), can efficiently quench the fluorescence of an oppositely charged red fluorescent phthalocyanine compound with a matched molecular structure, tetrasulfonated aluminum phthalocyanine (AlS4Pc), because of the formation of an ion pair complex (AlS4Pc-Alcian blue 8GX) that exhibits almost no fluorescence. An investigation was carried out on the fluorescence recovery of AlS4Pc-Alcian blue 8GX caused by a series of anionic surfactants containing a sulfonic group (sodium dodecylbenzenesulfonate (SDBS), sodium lauryl sulfate (SLS), and sodium dodecyl sulfate (SDS)). The results showed that SDBS exhibited a significant response, and the highest sensitivity among the surfactants. Due to its high efficiency of fluorescence quenching and the high level of fluorescence recovery, direct observes can even be performed by the naked eye. The results revealed that the Alcian blue 8GX-AlS4Pc ion-pair complex fluorescent probe only responded to SDBS in the low-concentration range. Based on the new founding, this study proposed a novel principle and method of fluorescence enhancement to specifically measure the concentration of SDBS, thereby achieving a highly sensitive and highly specific determination of SDBS. Under the optimal conditions, the fluorescence intensity (I(f)) of the system and the concentration of SDBS in the range of 1 × 10(-7) - 1 × 10(-5) mol/dm(3) exhibited a good linear relationship. This method is highly sensitive, and the operation is simple and rapid. It had been applied for the quantitative analysis of SDBS in environmental water, while achieving satisfactory results compared with those of the standard method. This study developed a new application of the fluorescent phthalocyanine compounds used as molecular probes in analytical sciences.
[Mh] Termos MeSH primário: Ânions
Benzenossulfonatos/química
Corantes Fluorescentes/química
Indóis/química
[Mh] Termos MeSH secundário: Azul Alciano/química
Cobre/química
Estrutura Molecular
Óptica e Fotônica
Compostos Organometálicos/química
Dodecilsulfato de Sódio/química
Solubilidade
Espectrometria de Fluorescência/métodos
Tensoativos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anions); 0 (Benzenesulfonates); 0 (Fluorescent Dyes); 0 (Indoles); 0 (Organometallic Compounds); 0 (Surface-Active Agents); 368GB5141J (Sodium Dodecyl Sulfate); 3VEX9T7UT5 (copper phthalocyanine); 47822-79-7 (aluminum phthalocyanine); 60NSK897G9 (dodecylbenzenesulfonic acid); 789U1901C5 (Copper); P4448TJR7J (Alcian Blue); V5PUF4VLGY (phthalocyanine)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:170103
[Lr] Data última revisão:
170103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160211
[St] Status:MEDLINE
[do] DOI:10.2116/analsci.32.201


  9 / 917 MEDLINE  
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[PMID]:26836512
[Au] Autor:Schroeder JT; Bieneman AP
[Ad] Endereço:Johns Hopkins University School of Medicine, Baltimore, Maryland.
[Ti] Título:Isolation of Human Basophils.
[So] Source:Curr Protoc Immunol;112:7.24.1-8, 2016 Feb 02.
[Is] ISSN:1934-368X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Isolating human basophils from blood has long been hampered by the fact that these granulocytes represent just 1% or less of the circulating leukocyte population. We describe herein laboratory protocols that have been refined over the past ∼25 years that now enable investigators to prepare basophils for use in a variety of assays to assess the in vitro biology of these immune cells, both in IgE -dependent and -independent responses.
[Mh] Termos MeSH primário: Basófilos/citologia
[Mh] Termos MeSH secundário: Azul Alciano
Separação Celular
Centrifugação
Corantes
Seres Humanos
Povidona
Dióxido de Silício
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Coloring Agents); 65455-52-9 (Percoll); 7631-86-9 (Silicon Dioxide); FZ989GH94E (Povidone); P4448TJR7J (Alcian Blue)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160203
[St] Status:MEDLINE
[do] DOI:10.1002/0471142735.im0724s112


  10 / 917 MEDLINE  
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[PMID]:26748699
[Au] Autor:Gholkar AA; Senese S; Lo YC; Vides E; Contreras E; Hodara E; Capri J; Whitelegge JP; Torres JZ
[Ad] Endereço:Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA.
[Ti] Título:The X-Linked-Intellectual-Disability-Associated Ubiquitin Ligase Mid2 Interacts with Astrin and Regulates Astrin Levels to Promote Cell Division.
[So] Source:Cell Rep;14(2):180-8, 2016 Jan 12.
[Is] ISSN:2211-1247
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mid1 and Mid2 are ubiquitin ligases that regulate microtubule dynamics and whose mutation is associated with X-linked developmental disorders. We show that astrin, a microtubule-organizing protein, co-purifies with Mid1 and Mid2, has an overlapping localization with Mid1 and Mid2 at intercellular bridge microtubules, is ubiquitinated by Mid2 on lysine 409, and is degraded during cytokinesis. Mid2 depletion led to astrin stabilization during cytokinesis, cytokinetic defects, multinucleated cells, and cell death. Similarly, expression of a K409A mutant astrin in astrin-depleted cells led to the accumulation of K409A on intercellular bridge microtubules and an increase in cytokinetic defects, multinucleated cells, and cell death. These results indicate that Mid2 regulates cell division through the ubiquitination of astrin on K409, which is critical for its degradation and proper cytokinesis. These results could help explain how mutation of MID2 leads to misregulation of microtubule organization and the downstream disease pathology associated with X-linked intellectual disabilities.
[Mh] Termos MeSH primário: Azul Alciano/metabolismo
Ligases/metabolismo
Proteínas Associadas aos Microtúbulos/genética
Proteínas Associadas aos Microtúbulos/metabolismo
Fenazinas/metabolismo
Fenotiazinas/metabolismo
Resorcinóis/metabolismo
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
Ubiquitina/metabolismo
[Mh] Termos MeSH secundário: Divisão Celular
Citocinese
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (MID2 protein, human); 0 (Microtubule-Associated Proteins); 0 (Phenazines); 0 (Phenothiazines); 0 (Resorcinols); 0 (Transcription Factors); 0 (Ubiquitin); 83097-09-0 (astrin); EC 6.- (Ligases); P4448TJR7J (Alcian Blue)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160111
[St] Status:MEDLINE



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