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[PMID]:28854790
[Au] Autor:Habip Z; Sohrabi P; Saribas S; Caliskan R; Demirci M; Karakullukcu A; Atalik K; Yuksel P; Uysal O; Kosan E; Bahar Tokman H; Kocazeybek B
[Ti] Título:Neopterin and soluble CD14 levels as indicators of immune activation in cases with low anti-HCV reactivity and true HCV infection.
[So] Source:Acta Virol;61(3):264-272, 2017.
[Is] ISSN:0001-723X
[Cp] País de publicação:Slovakia
[La] Idioma:eng
[Ab] Resumo:Neopterin and soluble CD14 (sCD14) are detected at high levels in hepatitis C virus (HCV) infections. We aimed to evaluate the role of these plasma immune activation biomarkers, for the indirect assessment of immune activation status of patients with low anti-HCV reactivity and a HCV infection. Low anti-HCV reactivity group (LRG, n: 70), true positive HCV infection group (THG, 30) and healthy control group (HCG, 30) were analyzed in this study. We have used ELISA, HCV RIBA/LIA and HCV-RNA methods. Mean neopterin levels were significantly lower in LRG than THG (p <0.001). In contrast, those values were not significantly different from those of HCG (p >0.05). Mean sCD14 were significantly higher in LRG than THG and HCG (p <0.05, p <0.001). Values of 3.95 µg/ml and 5.36 nmol/l for sCD14 and neopterin resulted in the maximum area under the receiver operating characteristic curves (ROC), which were 0.859 (95% CI, 0.745 to 0.935; <0.0001) and 0.788 (95% CI, 0.663 to 0.883; <0.0001), respectively. These cut-offs corresponded to a sensitivity of 73.3% and a specificity of 73.3% for neopterin and of 100% and 76.7% for sCD14. Our results suggest that a specific immunoactivation might be caused by true positive HCV infection. Due to the significant results sCD14 in LRG might be non-specifically affected by some underlying atypical immunohematological pathologies. Only neopterin might be used to exclude low anti-HCV reactivity from a true HCV infection. The use of neopterin but not sCD14 in combination with fourth-generation EIA/CMIA combo tests will be useful when nucleic acid tests are not available for screening blood donors at blood banks.
[Mh] Termos MeSH primário: Hepacivirus/imunologia
Hepatite C/imunologia
Receptores de Lipopolissacarídeos/imunologia
Receptores de Lipopolissacarídeos/metabolismo
Neopterina/imunologia
Neopterina/metabolismo
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Biomarcadores/metabolismo
Estudos de Casos e Controles
Estudos Transversais
Feminino
Hepatite C/metabolismo
Seres Humanos
Masculino
Meia-Idade
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY
[Nm] Nome de substância:
0 (Biomarkers); 0 (Lipopolysaccharide Receptors); 670-65-5 (Neopterin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.4149/av_2017_304


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[PMID]:28592710
[Au] Autor:Sipahi H; Girgin G; Palabiyik SS; Tutkun E; Yilmaz OH; Baydar T
[Ad] Endereço:Department of Toxicology, Faculty of Pharmacy, University of Yeditepe.
[Ti] Título:Possible changes of New-Generation inflammation markers with occupational lead exposure.
[So] Source:J Occup Health;59(4):345-351, 2017 Jul 27.
[Is] ISSN:1348-9585
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: Occupational lead (Pb) exposure is still an important health problem in the world. Long-term Pb exposure causes several adverse effects. The aim of this study was to investigate the changes of inflammation markers with chronic Pb exposure by analyzing neopterin levels and kynurenine (Kyn) to tryptophan (Trp) ratio that reflects indolamine 2,3-dioxygenase activity and to compare with healthy volunteers' parameters. METHODS: Blood lead levels (BLLs) were analyzed by atomic absorption spectrometry. Urinary neopterin and serum Kyn and Trp levels were analyzed by high-performance liquid chromatography. RESULTS: According to our results, mean BLL of the 29 workers was 20.4±9.6 µg/dl. Urinary neopterin levels, serum Kyn levels, and Kyn/Trp of Pb workers (188±52 µmol/mol creatinine, 2.70±0.66 µM, and 43.19±10.38 µmol/mmol, respectively) were significantly higher than controls (144±35 µmol/mol creatinine, 2.08±0.34 µM, and 32.24±7.69 µmol/mmol, respectively). Pb-exposed workers were divided into further three groups according to their BLLs: as 10-19 µg/dl (n=18), 20-29 µg/dl (n=8), and 30-49 µg/dl (n=3). Neopterin levels of the workers with BLL of 30-49 µg/dl were significantly higher than those of BLL with 10-29 µg/dl, while Trp levels decreased. Kyn/Trp of workers with BLL of 30-49 µg/dl were elevated significantly compared with the workers with BLL<30 µg/dl. In addition to neopterin, Kyn and Kyn/Trp levels were positively influenced by Pb exposure. CONCLUSIONS: Increased level of inflammation markers confirms the adverse effects of Pb even low BLLs, and we suggest that monitoring BLLs with inflammation markers could help to prevent serious occupational health problems.
[Mh] Termos MeSH primário: Cinurenina/sangue
Chumbo/sangue
Neopterina/urina
Exposição Ocupacional/análise
Triptofano/sangue
[Mh] Termos MeSH secundário: Adulto
Biomarcadores/sangue
Biomarcadores/urina
Cromatografia Líquida de Alta Pressão
Hospitais
Seres Humanos
Indolamina-Pirrol 2,3,-Dioxigenase
Inflamação/sangue
Inflamação/urina
Masculino
Meia-Idade
Exposição Ocupacional/efeitos adversos
Turquia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Indoleamine-Pyrrole 2,3,-Dioxygenase); 2P299V784P (Lead); 343-65-7 (Kynurenine); 670-65-5 (Neopterin); 8DUH1N11BX (Tryptophan)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170908
[Lr] Data última revisão:
170908
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170609
[St] Status:MEDLINE
[do] DOI:10.1539/joh.16-0273-OA


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[PMID]:28592604
[Au] Autor:Colston JM; Peñataro Yori P; Colantuoni E; Moulton LH; Ambikapathi R; Lee G; Rengifo Trigoso D; Siguas Salas M; Kosek MN
[Ad] Endereço:Departments of International Health and.
[Ti] Título:A methodologic framework for modeling and assessing biomarkers of environmental enteropathy as predictors of growth in infants: an example from a Peruvian birth cohort.
[So] Source:Am J Clin Nutr;106(1):245-255, 2017 Jul.
[Is] ISSN:1938-3207
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Environmental enteropathy (EE) impairs the gut's absorptive capacity and immune function and causes decelerations in statural growth that manifest gradually over time. To illustrate an approach for assessing emerging biomarkers of EE, we separately assessed the associations between 3 such markers and subsequent nutritional status. Stool samples were routinely collected between January 2010 and November 2014 from a cohort of 303 Peruvian infants and analyzed for concentrations of the biomarkers α-1-antitrypsin (AAT), myeloperoxidase, and neopterin. For each marker, a mixed-effects linear regression model was fitted for length-for-age scores (LAZs) obtained from anthropometric assessments that incorporated covariate predictors, polynomial terms for age, and product interaction terms to test associations over varying lag lengths. The biomarkers' contribution to the models was assessed with the use of the likelihood ratio test and partial statistics. Test statistics for the combined inclusion of the 4-model terms that involved the biomarker were highly statistically significant for AAT (28.71; < 0.0001) and myeloperoxidase (62.79; < 0.0001) over a 3-mo lag and moderately so for neopterin (13.97; = 0.0074). AAT and myeloperoxidase seemed to interact strongly with age, with the magnitude and direction of the effect varying considerably over the first 3 y of life. The largest proportion of the variance explained by any biomarker (2.8%) and the largest difference in LAZ predicted between the 5th and 95th percentile (0.25) was by myeloperoxidase over a 2-mo lag. Of the 3 fecal biomarkers studied, 2 that related to intestinal function-AAT and myeloperoxidase-were associated with small but highly statistically significant differences in future statural growth trajectories in infants in this cohort, lending further evidence to the EE hypothesis that increased gut permeability and inflammation adversely affects subsequent nutritional status. This association exhibited a complex interaction with age. This trial was registered at clinicaltrials.gov as NCT02441426.
[Mh] Termos MeSH primário: Proteínas de Transporte/metabolismo
Transtornos do Crescimento/etiologia
Infecção/complicações
Enteropatias/complicações
Neopterina/metabolismo
Estado Nutricional
Peroxidase/metabolismo
[Mh] Termos MeSH secundário: Biomarcadores/metabolismo
Estatura
Pré-Escolar
Meio Ambiente
Exposição Ambiental
Fezes
Feminino
Transtornos do Crescimento/metabolismo
Seres Humanos
Lactente
Infecção/metabolismo
Infecção/patologia
Inflamação
Enteropatias/metabolismo
Enteropatias/patologia
Intestinos/metabolismo
Intestinos/patologia
Masculino
Desnutrição/complicações
Modelos Biológicos
Permeabilidade
Peru
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (C3orf15 protein, human); 0 (Carrier Proteins); 670-65-5 (Neopterin); EC 1.11.1.7 (Peroxidase)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170609
[St] Status:MEDLINE
[do] DOI:10.3945/ajcn.116.151886


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[PMID]:28524767
[Au] Autor:Valdiglesias V; Sánchez-Flores M; Maseda A; Lorenzo-López L; Marcos-Pérez D; López-Cortón A; Strasser B; Fuchs D; Laffon B; Millán-Calenti JC; Pásaro E
[Ad] Endereço:a Universidade da Coruña, DICOMOSA Group , Department of Psychology, Area of Psychobiology , A Coruña , Spain.
[Ti] Título:Immune biomarkers in older adults: Role of physical activity.
[So] Source:J Toxicol Environ Health A;80(13-15):605-620, 2017.
[Is] ISSN:1528-7394
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Aging is associated with a decline in the normal functioning of the immune system. Several studies described the relationship between immunological alterations, including immunosenescence and inflammation, and aging or age-related outcomes, such as sarcopenia, depression, and neurodegenerative disorders. Physical activity is known to improve muscle function and to exert a number of benefits on older adult health, including reduced risk for heart and metabolic system chronic diseases. However, the positive influence of physical activity on the immune system has not been elucidated. In order to shed light on the role of physical activity in immune responses of older individuals, a number of immunological parameters comprising % lymphocyte subsets (CD3 , CD4 , CD8 , CD19 , and CD16 56 ) and serum levels of neopterin and tryptophan metabolism products were evaluated in peripheral blood samples of older adults performing normal (N = 170) or reduced (N = 89) physical activity. In addition, the potential influence of other clinical and epidemiological factors was also considered. Results showed that subjects with reduced physical activity displayed significantly higher levels of CD4 /CD8 ratio, kynurenine/tryptophan ratio, and serum neopterin, along with lower %CD19 cells and tryptophan concentrations. Further, some immunological biomarkers were associated with cognitive impairment and functional status. These data contribute to reinforce the postulation that physical activity supports healthy aging, particularly by helping to protect the immunological system from aging-related changes.
[Mh] Termos MeSH primário: Exercício
Sistema Imunitário/fisiologia
Subpopulações de Linfócitos/fisiologia
[Mh] Termos MeSH secundário: Atividades Cotidianas
Fatores Etários
Idoso
Idoso de 80 Anos ou mais
Envelhecimento/imunologia
Envelhecimento/fisiologia
Biomarcadores/sangue
Relação CD4-CD8
Disfunção Cognitiva/sangue
Disfunção Cognitiva/imunologia
Exercício/fisiologia
Feminino
Seres Humanos
Cinurenina/sangue
Masculino
Neopterina/sangue
Inquéritos e Questionários
Triptofano/sangue
Triptofano/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 343-65-7 (Kynurenine); 670-65-5 (Neopterin); 8DUH1N11BX (Tryptophan)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170520
[St] Status:MEDLINE
[do] DOI:10.1080/15287394.2017.1286898


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[PMID]:28495136
[Au] Autor:Raheja UK; Fuchs D; Lowry CA; Stephens SH; Pavlovich MA; Mohyuddin H; Yousufi H; Ryan KA; O'Connell J; Brenner LA; Punzalan C; Hoisington AJ; Nijjar GK; Groer M; Shuldiner AR; Pollin TI; Stiller JW; Mitchell BD; Postolache TT
[Ad] Endereço:Mood and Anxiety Program, University of Maryland School of Medicine, 685 W. Baltimore Street, Suite# 930, Baltimore, MD 21201, USA; Child and Adolescent Psychiatry Residency Program, Department of Psychiatry and Behavioral Sciences, Emory University School of Medicine, 12 Executive Park Drive, Atlan
[Ti] Título:Heritability of plasma neopterin levels in the Old Order Amish.
[So] Source:J Neuroimmunol;307:37-41, 2017 Jun 15.
[Is] ISSN:1872-8421
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: We examined the heritability of neopterin, a biomarker for cell-mediated immunity and oxidative stress, and potentially for psychiatric disorders, in the Old Order Amish. METHODS: Plasma neopterin levels were determined in 2015 Old Order Amish adults. Quantitative genetic procedures were used to estimate heritability of neopterin. RESULTS: Heritability of log-neopterin was estimated at 0.07 after adjusting for age, gender, and household (p=0.03). The shared household effect was 0.06 (p<0.02). CONCLUSIONS: We found a low heritability of neopterin and small household effect, suggesting that non-household environmental factors are more important determinants of variance of neopterin levels in the Amish.
[Mh] Termos MeSH primário: Envelhecimento/sangue
Amish/estatística & dados numéricos
Neopterina/sangue
[Mh] Termos MeSH secundário: Adulto
Envelhecimento/imunologia
Meio Ambiente
Feminino
Seres Humanos
Masculino
Meia-Idade
Neopterina/genética
Estudos Retrospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
670-65-5 (Neopterin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE


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[PMID]:28397440
[Au] Autor:Sapa A; Rak A; Wybieralska M; Machon J; Krzywonos-Zawadzka A; Zawadzki K; Welna M; Wozniak M
[Ad] Endereço:Department of Clinical Chemistry, Wroclaw Medical University, Poland.
[Ti] Título:Diagnostic usefulness of sCD163, procalcitonin and neopterin for sepsis risk assessment in critically ill patients.
[So] Source:Adv Clin Exp Med;26(1):101-108, 2017 Jan-Feb.
[Is] ISSN:1899-5276
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Sepsis is one of the most common causes of hospitalization and it is characterized by a high mortality rate in spite of the great progress in diagnosis and treatment achieved in recent years. Early diagnosis of sepsis is one of the most important elements of effective treatment. The clinical symptoms are not specific and biomarkers are considered to be useful tools in sepsis diagnostics. OBJECTIVES: The aim of our study was to evaluate the diagnostic value of sCD163 as a marker of sepsis and a comparison of it with procalcitonin and neopterin in ICU patients. MATERIAL AND METHODS: Concentrations of PCT, sCD163 and NPT were measured in 52 serum samples collected from 30 patients of the Department of Anesthesiology and Intensive Therapy of the University Hospital in Wroclaw. Venous blood was collected on the 1st and 3rd day of hospitalization. The Human CD163 Quantikine ELISA Kit was used to determine the concentrations of sCD163. Neopterin concentrations were measured by a Neopterin ELISA kit. PCT was measured at the University Center of Laboratory Diagnostics in Wroclaw using an automatic VIDAS® B.R.A.H.M.S. PCT assay. RESULTS: Our study showed that there was a significant difference between the values obtained in the study and the reference group for PCT (p < 0.0001), sCD163 (p = 0.0001) and NPT (p = 0.0001), whereas there was no difference observed between the samples obtained on the 1st and 3rd day (p = 0.5129). The area under the ROC curve was 0.847, and was comparable to the AUC of procalcitonin (0.840), and slightly higher than the AUC of neopterin (0.763), although these differences were not significant (p = 0.2990 and p = 0.9329, respectively). CONCLUSIONS: sCD163 and neopterin are promising parameters in the diagnosis of sepsis, and their value in the diagnosis of sepsis in critically ill patients may be comparable to procalcitonin.
[Mh] Termos MeSH primário: Antígenos CD/sangue
Antígenos de Diferenciação Mielomonocítica/sangue
Biomarcadores/sangue
Calcitonina/sangue
Neopterina/sangue
Receptores de Superfície Celular/sangue
Sepse/diagnóstico
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Área Sob a Curva
Estado Terminal
Diagnóstico Precoce
Ensaio de Imunoadsorção Enzimática
Feminino
Seres Humanos
Masculino
Meia-Idade
Curva ROC
Medição de Risco
Sensibilidade e Especificidade
Sepse/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD); 0 (Antigens, Differentiation, Myelomonocytic); 0 (Biomarkers); 0 (CD163 antigen); 0 (Receptors, Cell Surface); 670-65-5 (Neopterin); 9007-12-9 (Calcitonin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170622
[Lr] Data última revisão:
170622
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE
[do] DOI:10.17219/acem/63251


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[PMID]:28380116
[Au] Autor:Takatani M; Crispim ME; Fraiji N; Stefani MM; Kiesslich D
[Ad] Endereço:Universidade Federal do Amazonas, Faculdade de Medicina, Departamento de Clínica Cirúrgica, Manaus, Amazonas, Brazil.
[Ti] Título:Clinical and laboratory features of HTLV-I asymptomatic carriers and patients with HTLV-I-associated myelopathy/tropical spastic paraparesis from the Brazilian Amazon.
[So] Source:Rev Inst Med Trop Sao Paulo;59:e5, 2017 Apr 03.
[Is] ISSN:1678-9946
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Clinical and laboratory parameters including blood and cerebrospinal fluid (CSF) neopterin were investigated in human-T-lymphotropic-virus-type-I associated-myelopathy/tropical-spastic-paraparesis-HAM/TSP and in HTLV-I carriers. HAM/TSP (n = 11, 2 males/9 females, median age = 48 years), recently diagnosed HTLV-I carriers (n = 21, 15 females/6 males, median age = 44 years), healthy individuals (n = 20, 10 males/10 females, median age = 34.6 years) from the Brazilian Amazon (Manaus, Amazonas State) were investigated. Neopterin was measured (IBL ELISA Neopterin, Germany) in serum samples of all the participants, in CSF of 9 HAM/TSP patients as well as in 6 carriers. In HAM/TSP patients, CSF cell counts, protein and glucose were measured, the Osame's motor-disability-score/OMDS was determined, and brain/spinal cord magnetic-resonance-imaging (MRI) was performed. HAM/TSP patients had normal CSF glucose, leukocyte counts; and normal protein levels predominated. Brain-MRI showed white-matter lesions in 7 out of 11 HAM/TSP patients. OMDS varied from 2-8: 9 were able to walk, 2 were wheel-chair-users. The median serum neopterin concentration in HAM/TSP patients was 6.6 nmol/ L; min. 2.8- max. 12.5 nmol/ L); was lower in carriers (4.3 nmol/L; min. 2.7- max. 7.2 nmol/ L) as well as in healthy participants (4.7 nmol/ L; min. 2.7- max. 8.0 nmol/ L) (p < 0.05). CSF neopterin concentrations in HAM/TSP patients were higher than in serum samples, and higher compared to carriers (p < 0.05). Carriers had similar serum-CSF neopterin concentrations compared to healthy participants. Variable clinical and laboratory profiles were seen in HAM/TSP patients, however our results support the neopterin measurement as a potential biomarker of disease activity.
[Mh] Termos MeSH primário: Vírus 1 Linfotrópico T Humano
Neopterina/sangue
Neopterina/líquido cefalorraquidiano
Paraparesia Espástica Tropical/sangue
Paraparesia Espástica Tropical/líquido cefalorraquidiano
[Mh] Termos MeSH secundário: Adulto
Idoso
Biomarcadores/sangue
Biomarcadores/líquido cefalorraquidiano
Brasil
Portador Sadio
Estudos de Casos e Controles
Feminino
Seres Humanos
Imagem por Ressonância Magnética
Masculino
Meia-Idade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 670-65-5 (Neopterin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170601
[Lr] Data última revisão:
170601
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170406
[St] Status:MEDLINE


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[PMID]:28109742
[Au] Autor:Santagata S; Di Carlo E; Carducci C; Leuzzi V; Angeloni A; Carducci C
[Ad] Endereço:Department of Experimental Medicine, Sapienza University of Rome, Viale del Policlinico 155, 00161 Rome, Italy. Electronic address: silviasantagata1@gmail.com.
[Ti] Título:Development of a new UPLC-ESI-MS/MS method for the determination of biopterin and neopterin in dried blood spot.
[So] Source:Clin Chim Acta;466:145-151, 2017 Mar.
[Is] ISSN:1873-3492
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: (6R)-5,6,7,8-tetrahydrobiopterin (BH4) deficiencies are rare inherited defects of synthesis or regeneration of BH4. Due to the resulting hyperphenylalaninemia (HPA), some of them are detected by newborn screening and require the assessment of the pattern of neopterin (Neo) and biopterin (Bio) excretion in urine to be confirmed. Aim of present study was to develop a method for the measurement of these diagnostic biomarkers in dried blood spot (DBS). METHODS: After DBS extraction, samples were filtered and injected into the UPLC column coupled with a tandem mass spectrometer working in positive electrospray ionization. RESULTS: The chromatographic separation was accomplished in 6min. The LoQ was 0.57 and 1.45nmol/l of blood for Neo and Bio respectively and the response was linear over the range 0-100nmol/l of blood. The within- and between-day imprecision was <6.4 and 10.8% respectively. Reference ranges for newborns, infants and children/adult were established. The method was tested in 11 patients affected by BH4 defects. CONCLUSIONS: The assessment of Neo and Bio in DBS is reliable and sensitive and may be proposed as a second tier test for the newborns with hyperphenylalaninemia (HPA) as well as a new potential diagnostic tool for symptomatic subjects with BH4 deficiencies.
[Mh] Termos MeSH primário: Biopterina/sangue
Teste em Amostras de Sangue Seco/métodos
Neopterina/sangue
Fenilcetonúrias/diagnóstico
[Mh] Termos MeSH secundário: Adolescente
Criança
Pré-Escolar
Cromatografia Líquida/métodos
Teste em Amostras de Sangue Seco/normas
Seres Humanos
Lactente
Recém-Nascido
Triagem Neonatal/métodos
Fenilcetonúrias/sangue
Valores de Referência
Espectrometria de Massas em Tandem/métodos
Espectrometria de Massas em Tandem/normas
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
22150-76-1 (Biopterin); 670-65-5 (Neopterin)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170826
[Lr] Data última revisão:
170826
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170123
[St] Status:MEDLINE


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[PMID]:28107600
[Au] Autor:Pichler R; Fritz J; Heidegger I; Steiner E; Culig Z; Klocker H; Fuchs D
[Ad] Endereço:Urological Laboratory and Division of Experimental Urology, Department of Urology, Biocenter, Medical University Innsbruck, Innsbruck, Austria.
[Ti] Título:Predictive and prognostic role of serum neopterin and tryptophan breakdown in prostate cancer.
[So] Source:Cancer Sci;108(4):663-670, 2017 Apr.
[Is] ISSN:1349-7006
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The γ-interferon-induced enzymes indoleamine 2,3-dioxygenase and GTP-cyclohydrolase are key players in tumor immune escape mechanisms. We quantified serum levels of neopterin and tryptophan breakdown (tryptophan, kynurenine, and kynurenine-to-tryptophan ratio) in addition to prostate-specific antigen (PSA) in newly diagnosed prostate cancer (PCa) patients (n = 100) before radical prostatectomy (RP) as well as at time of biochemical recurrence (BCR) after RP (n = 50) in comparison to healthy men (n = 49). Effects of biomarkers on the risk of PCa diagnosis on transrectal biopsy, worse histopathological characteristics of the RP specimens, and cancer-specific survival (CSS) after BCR were investigated. Neopterin (hazard ratio [HR], 2.46; 95% confidence interval [CI], 1.08-5.61; P = 0.032) and kynurenine (HR, 2.93; 95% CI, 1.26-6.79; P = 0.012) levels were univariately associated with CSS. When adjusted for other biomarkers, only neopterin remained an independent predictor of CSS (HR, 2.56; 95% CI, 1.07-6.12; P = 0.035). Only PSA was associated with an increased risk of PCa diagnosis on biopsy, univariately (odds ratio, 3.14; 95% CI, 1.68-5.88; P < 0.001) as well when adjusted for other biomarkers (odds ratio, 3.29; 95% CI, 1.70-6.35; P < 0.001). Moreover, only preoperative PSA was able to predict positive surgical margin (area under the receiver operating characteristic curve [AUC] = 0.71; 95% CI, 0.59-0.82; P = 0.001), higher Gleason score (AUC = 0.75; 95% CI, 0.66-0.85; P < 0.001) and extraprostatic involvement (AUC = 0.79; 95% CI, 0.69-0.88; P < 0.001) at RP specimens, respectively. Although serum neopterin and tryptophan breakdown cannot be considered as biomarkers in detecting PCa or in predicting worse final pathological findings, neopterin levels are useful for stratifying patients into different prognostic groups after BCR.
[Mh] Termos MeSH primário: Cinurenina/sangue
Neopterina/sangue
Neoplasias da Próstata/sangue
Triptofano/sangue
[Mh] Termos MeSH secundário: Adulto
Idoso
Biomarcadores Tumorais/sangue
Seres Humanos
Estimativa de Kaplan-Meier
Modelos Logísticos
Masculino
Meia-Idade
Análise Multivariada
Avaliação de Resultados (Cuidados de Saúde)/métodos
Avaliação de Resultados (Cuidados de Saúde)/estatística & dados numéricos
Período Pós-Operatório
Valor Preditivo dos Testes
Período Pré-Operatório
Prognóstico
Modelos de Riscos Proporcionais
Antígeno Prostático Específico/sangue
Prostatectomia
Neoplasias da Próstata/diagnóstico
Neoplasias da Próstata/cirurgia
Curva ROC
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 343-65-7 (Kynurenine); 670-65-5 (Neopterin); 8DUH1N11BX (Tryptophan); EC 3.4.21.77 (Prostate-Specific Antigen)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170121
[St] Status:MEDLINE
[do] DOI:10.1111/cas.13171


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[PMID]:28005163
[Au] Autor:Pichler R; Gruenbacher G; Culig Z; Brunner A; Fuchs D; Fritz J; Gander H; Rahm A; Thurnher M
[Ad] Endereço:Department of Urology, Research Group of Urologic Oncology, Medical University of Innsbruck, Anichstrasse 35, 6020, Innsbruck, Austria. Renate.Pichler@i-med.ac.at.
[Ti] Título:Intratumoral Th2 predisposition combines with an increased Th1 functional phenotype in clinical response to intravesical BCG in bladder cancer.
[So] Source:Cancer Immunol Immunother;66(4):427-440, 2017 Apr.
[Is] ISSN:1432-0851
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Th1-type immunity is considered to be required for efficient response to BCG in bladder cancer, although Th2 predisposition of BCG responders has recently been reported. The aim was to evaluate the relationship of Th1 and Th2 components in 23 patients undergoing BCG treatment. Peripheral blood, serum and urine samples were prospectively collected at baseline, during and after BCG. Th1 (neopterin, tryptophan, kynurenine, kynurenine-to-tryptophan ratio (KTR), IL-12, IFN-γ, soluble TNF-R75 and IL-2Rα) and Th2 (IL-4, IL-10) biomarkers as well as CD4 expression in T helper (Th), effector and regulatory T cells were determined. Local immune cell subsets were measured on formalin-fixed, paraffin-embedded cancer tissue by immunohistochemistry to examine expression of transcription factors that control Th1 (T-bet) and Th2-type (GATA3) immunity. We confirmed a Th2 predisposition with a mean GATA3/T-bet ratio of 5.51. BCG responders showed significantly higher levels of urinary (p = 0.003) and serum neopterin (p = 0.012), kynurenine (p = 0.015), KTR (p = 0.005), IFN-γ (p = 0.005) and IL-12 (p = 0.003) during therapy, whereas levels of IL-10 decreased significantly (p < 0.001) compared to non-responders. GATA3/T-bet ratio correlated positively with serum neopterin (p = 0.008), IFN-γ (p = 0.013) and KTR (p = 0.018) after the first BCG instillation. We observed a significant increase in CD4 expression in the Th cell population (p < 0.05), with only a modest tendency toward higher frequency in responders compared to non-responders (p = 0.303). The combined assessment of GATA3/T-bet ratio, neopterin and KTR may be a useful biomarker in predicting BCG response. Th2-promoting factors such as GATA3 may trigger Th1-type immune responses and thus contribute to the BCG success.
[Mh] Termos MeSH primário: Imunoterapia/métodos
Mycobacterium bovis/imunologia
Células Th1/imunologia
Células Th2/imunologia
Neoplasias da Bexiga Urinária/terapia
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Biomarcadores/metabolismo
Citocinas/metabolismo
Feminino
Fator de Transcrição GATA3
Seres Humanos
Masculino
Meia-Idade
Neopterina/metabolismo
Proteínas com Domínio T-Box/genética
Proteínas com Domínio T-Box/metabolismo
Equilíbrio Th1-Th2
Resultado do Tratamento
Neoplasias da Bexiga Urinária/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Cytokines); 0 (GATA3 Transcription Factor); 0 (GATA3 protein, human); 0 (T-Box Domain Proteins); 0 (T-box transcription factor TBX21); 670-65-5 (Neopterin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161223
[St] Status:MEDLINE
[do] DOI:10.1007/s00262-016-1945-z



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