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  1 / 2032 MEDLINE  
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[PMID]:29273417
[Au] Autor:Cherukupalli S; Hampannavar GA; Chinnam S; Chandrasekaran B; Sayyad N; Kayamba F; Reddy Aleti R; Karpoormath R
[Ad] Endereço:Department of Pharmaceutical Chemistry, College of Health Sciences, University of KwaZulu-Natal, Durban 4000, South Africa.
[Ti] Título:An appraisal on synthetic and pharmaceutical perspectives of pyrazolo[4,3-d]pyrimidine scaffold.
[So] Source:Bioorg Med Chem;26(2):309-339, 2018 01 15.
[Is] ISSN:1464-3391
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Pyrazolo[4,3-d]pyrimidine, a fused heterocycle bearing pyrazole and pyrimidine portions has gained a significant attention in the field of bioorganic and medicinal chemistry. Pyrazolo[4,3-d]pyrimidine derivatives have demonstrated numerous pharmacological activities particularly, anti-cancer, anti-infectious, phosphodiesterase inhibitors, adenosine antagonists and cytokinin antagonists etc. This review extensively unveils the synthetic and pharmacological diversity with special emphasis on structural variations around pyrazolo[4,3-d]pyrimidine scaffold. This endeavour has thus uncovered the medicinal worthiness of pyrazolo[4,3-d]pyrimidine framework. To the best of our knowledge this review is the first compilation on synthetic, medicinal and structure activity relationship (SAR) aspects of pyrazolo[4,3-d]pyrimidines since 1956.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Antineoplásicos/farmacologia
Inibidores Enzimáticos/farmacologia
Neoplasias/tratamento farmacológico
Pirazóis/farmacologia
Pirimidinas/farmacologia
[Mh] Termos MeSH secundário: Adenosina/antagonistas & inibidores
Adenosina/metabolismo
Anti-Infecciosos/síntese química
Anti-Infecciosos/química
Antineoplásicos/síntese química
Antineoplásicos/química
Citocininas/antagonistas & inibidores
Citocininas/metabolismo
Inibidores Enzimáticos/síntese química
Inibidores Enzimáticos/química
Seres Humanos
Estrutura Molecular
Diester Fosfórico Hidrolases/metabolismo
Pirazóis/síntese química
Pirazóis/química
Pirimidinas/síntese química
Pirimidinas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Antineoplastic Agents); 0 (Cytokinins); 0 (Enzyme Inhibitors); 0 (Pyrazoles); 0 (Pyrimidines); 271-80-7 (pyrazolo(3,4-d)pyrimidine); EC 3.1.4.- (Phosphoric Diester Hydrolases); K72T3FS567 (Adenosine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171224
[St] Status:MEDLINE


  2 / 2032 MEDLINE  
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[PMID]:28467152
[Au] Autor:Kushwah S; Laxmi A
[Ad] Endereço:a National Institute of Plant Genome Research , New Delhi , India.
[Ti] Título:The interaction between glucose and cytokinin signaling in controlling Arabidopsis thaliana seedling root growth and development.
[So] Source:Plant Signal Behav;12(5):e1312241, 2017 May 04.
[Is] ISSN:1559-2324
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cytokinin (CK) and glucose (GLC) control several common responses in plants. There is an extensive overlap between CK and GLC signal transduction pathways in Arabidopsis. Physiologically, both GLC and CK could regulate root length in light. CK interacts with GLC via HXK1 dependent pathway for root length control. Wild-type (WT) roots cannot elongate in the GLC free medium while CK-receptor mutant ARABIDOPSIS HISTIDINE KINASE4 (ahk4) and type B ARR triple mutant ARABIDOPSIS RESPONSE REGULATOR1, 10,11 (arr1, 10,11) roots could elongate even in the absence of GLC as compared with the WT. The root hair initiation was also found defective in CK signaling mutants ahk4, arr1,10,11 and arr3,4,5,6,8,9 on increasing GLC concentration (up to 3%); and lesser number of root hairs were visible even at 5% GLC as compared with the WT. Out of 941 BAP regulated genes, 103 (11%) genes were involved in root growth and development. Out of these 103 genes, 60 (58%) genes were also regulated by GLC. GLC could regulate 5736 genes, which include 327 (6%) genes involved in root growth and development. Out of these 327 genes, 60 (18%) genes were also regulated by BAP. Both GLC and CK signaling cannot alter root length in light in auxin signaling mutant AUXIN RESPONSE3/INDOLE-3-ACETIC ACID17 (axr3/iaa17) suggesting that they may involve auxin signaling component as a nodal point. Therefore CK- and GLC- signaling are involved in controlling different aspects of root growth and development such as root length, with auxin signaling components working as downstream target.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/crescimento & desenvolvimento
Arabidopsis/metabolismo
Citocininas/metabolismo
Glucose/metabolismo
Plântulas/crescimento & desenvolvimento
Plântulas/metabolismo
[Mh] Termos MeSH secundário: Arabidopsis/genética
Proteínas de Arabidopsis/genética
Proteínas de Ligação a DNA/genética
Proteínas de Ligação a DNA/metabolismo
Regulação da Expressão Gênica de Plantas/genética
Regulação da Expressão Gênica de Plantas/fisiologia
Proteínas Nucleares/genética
Proteínas Nucleares/metabolismo
Proteínas Quinases/genética
Proteínas Quinases/metabolismo
Receptores de Superfície Celular/genética
Receptores de Superfície Celular/metabolismo
Plântulas/genética
Transdução de Sinais/genética
Transdução de Sinais/fisiologia
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ARR1 protein, Arabidopsis); 0 (ARR10 protein, Arabidopsis); 0 (ARR11 protein, Arabidopsis); 0 (AXR3 protein, Arabidopsis); 0 (Arabidopsis Proteins); 0 (Cytokinins); 0 (DNA-Binding Proteins); 0 (Nuclear Proteins); 0 (Receptors, Cell Surface); 0 (Transcription Factors); EC 2.7.- (Protein Kinases); EC 2.7.3.- (WOL protein, Arabidopsis); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171218
[Lr] Data última revisão:
171218
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1080/15592324.2017.1312241


  3 / 2032 MEDLINE  
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[PMID]:28778443
[Au] Autor:Yao X; Liu S; Ding W; Yue P; Jiang Q; Zhao M; Hu F; Zhang H
[Ad] Endereço:Department of Neurosurgery, Tongji Hospital, Tongji Medical College, Huazhong, University of Science and Technology, Wuhan, 430030, PR China.
[Ti] Título:TLR4 signal ablation attenuated neurological deficits by regulating microglial M1/M2 phenotype after traumatic brain injury in mice.
[So] Source:J Neuroimmunol;310:38-45, 2017 Sep 15.
[Is] ISSN:1872-8421
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Traumatic brain injury (TBI) initiates inflammatory responses that result in an enduring cascade of secondary neuronal loss and behavioural impairment. Toll-like receptor 4 (TLR4), predominantly expressed by microglia, recognizes damage-associated molecular patterns (DAMPs) and regulates inflammatory processes. Interestingly, the switch of microglial M1/M2 phenotypes after TBI is highly important regarding damage and restoration of neurological function. Therefore, we investigated the role and mechanisms of the TLR4 signalling pathway in regulating microglial M1/M2 phenotypes. Using a controlled cortical impact (CCI) model, we found that TLR4 knockout (KO) mice exhibited decreased infarct volumes and improved outcomes in behavioural tests. In addition, mice lacking TLR4 had higher expression of M2 phenotype biomarkers but lower expression of M1 phenotype biomarkers. Compared with microglia derived from wild-type (WT) mice, increased expression of M2 phenotype biomarkers and decreased expression of M1 phenotype biomarkers were also noted in primary cultures of microglia from TLR4 KO mice. In TLR4 KO mice, the expression levels of downstream signalling molecules of TLR4, such as active Rac-1 and phospho-AKT, were higher, while MyD88 and phospho-NF-κB p65 expression levels were lower than in WT mice. Our results demonstrate that the absence of TLR4 induces microglial polarization toward the M2 phenotype and promotes microglial migration and, in turn, alleviates the development of neuroinflammation, which indicates potential neuroprotective effects in the TBI mouse model. Furthermore, up-regulation of IL-4 expression in TLR4 KO mice could contribute to anti-inflammatory functions and promote microglial polarization toward the M2 phenotype, which might be mediated by active Rac-1 expression. Taken together, TLR4 deficiency contributes to regulating microglia to switch to the M2 phenotype, which ameliorates neurological impairment after TBI.
[Mh] Termos MeSH primário: Lesões Encefálicas Traumáticas/complicações
Lesões Encefálicas Traumáticas/patologia
Citocininas/metabolismo
Microglia/metabolismo
Transdução de Sinais/genética
Receptor 4 Toll-Like/deficiência
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Edema Encefálico/etiologia
Edema Encefálico/genética
Infarto Encefálico/etiologia
Movimento Celular/genética
Polaridade Celular/genética
Células Cultivadas
Citocininas/genética
Modelos Animais de Doenças
Regulação da Expressão Gênica/genética
Masculino
Aprendizagem em Labirinto/fisiologia
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Microglia/classificação
Doenças do Sistema Nervoso/etiologia
Doenças do Sistema Nervoso/genética
Navegação Espacial
Receptor 4 Toll-Like/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokinins); 0 (Tlr4 protein, mouse); 0 (Toll-Like Receptor 4)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170806
[St] Status:MEDLINE


  4 / 2032 MEDLINE  
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[PMID]:28737742
[Au] Autor:Osugi A; Kojima M; Takebayashi Y; Ueda N; Kiba T; Sakakibara H
[Ad] Endereço:RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro, Tsurumi, Yokohama 230-0045, Japan.
[Ti] Título:Systemic transport of trans-zeatin and its precursor have differing roles in Arabidopsis shoots.
[So] Source:Nat Plants;3:17112, 2017 Jul 24.
[Is] ISSN:2055-0278
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Organ-to-organ signal transmission is essential for higher organisms to ensure coordinated biological reactions during metabolism and morphogenesis. Similar to organs in animals, plant organs communicate by various signalling molecules. Among them, cytokinins, a class of phytohormones, play a key role as root-to-shoot long-distance signals, regulating various growth and developmental processes in shoots . Previous studies have proposed that trans-zeatin-riboside, a type of cytokinin precursor, is a major long-distance signalling form in xylem vessels and its action depends on metabolic conversion via the LONELY GUY enzyme in proximity to the site of action . Here we report an additional long-distance signalling form of cytokinin: trans-zeatin, an active form. Grafting between various cytokinin biosynthetic and transportation mutants revealed that root-to-shoot translocation of trans-zeatin, a minor component of xylem cytokinin, controls leaf size but not meristem activity-related traits, whereas that of trans-zeatin riboside is sufficient for regulating both traits. Considering the ratio of trans-zeatin to trans-zeatin-riboside in xylem and their delivery rate change in response to environmental conditions, this dual long-distance cytokinin signalling system allows plants to fine-tune the manner of shoot growth to adapt to fluctuating environments.
[Mh] Termos MeSH primário: Arabidopsis/metabolismo
Isopenteniladenosina/análogos & derivados
Brotos de Planta/metabolismo
Zeatina/metabolismo
[Mh] Termos MeSH secundário: Citocininas/metabolismo
Isopenteniladenosina/metabolismo
Transdução de Sinais
Xilema/metabolismo
Zeatina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokinins); 7724-76-7 (Isopentenyladenosine); 7I6OOJ9GR6 (Zeatin); 7LG4D082A9 (zeatin riboside)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170725
[St] Status:MEDLINE
[do] DOI:10.1038/nplants.2017.112


  5 / 2032 MEDLINE  
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[PMID]:28718648
[Au] Autor:Shiono K; Hashizaki R; Nakanishi T; Sakai T; Yamamoto T; Ogata K; Harada KI; Ohtani H; Katano H; Taira S
[Ad] Endereço:Department of Bioscience and Biotechnology, Fukui Prefectural University , 4-1-1 Matsuoka-Kenjojima, Eiheiji, Fukui 910-1195, Japan.
[Ti] Título:Multi-imaging of Cytokinin and Abscisic Acid on the Roots of Rice (Oryza sativa) Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry.
[So] Source:J Agric Food Chem;65(35):7624-7628, 2017 Sep 06.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plant hormones act as important signaling molecules that regulate responses to abiotic stress as well as plant growth and development. Because their concentrations of hormones control the physiological responses in the target tissue, it is important to know the distributions and concentrations in the tissues. However, it is difficult to determine the hormone concentration on the plant tissue as a result of the limitations of conventional methods. Here, we report the first multi-imaging of two plant hormones, one of cytokinin [i.e., trans-zeatin (tZ)] and abscisic acid (ABA) using a new technology, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) imaging. Protonated signals of tZ (m/z 220.1) and ABA (m/z 265.3) were chosen on longitudinal sections of rice roots for MS imaging. tZ was broadly distributed about 40 mm behind the root apex but was barely detectable at the apex, whereas ABA was mainly detected at the root apex. Multi-imaging using MALDI-TOF-MS enabled the visualization of the localization and quantification of plant hormones. Thus, this tool is applicable to a wide range of plant species growing under various environmental conditions.
[Mh] Termos MeSH primário: Ácido Abscísico/metabolismo
Citocininas/metabolismo
Oryza/metabolismo
Reguladores de Crescimento de Planta/metabolismo
Raízes de Plantas/química
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
[Mh] Termos MeSH secundário: Transporte Biológico
Oryza/química
Raízes de Plantas/metabolismo
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokinins); 0 (Plant Growth Regulators); 72S9A8J5GW (Abscisic Acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170719
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b02255


  6 / 2032 MEDLINE  
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[PMID]:28665127
[Au] Autor:Mik V; Micková Z; Dolezal K; Frébort I; Pospísil T
[Ad] Endereço:Department of Chemical Biology and Genetics and ‡Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University , Olomouc 771 47, Czech Republic.
[Ti] Título:Activity of (+)-Discadenine as a Plant Cytokinin.
[So] Source:J Nat Prod;80(7):2136-2140, 2017 Jul 28.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Discadenine (1), a self-spore germination inhibitor from the cellular slim mold Dictyostelium discoideum, is structurally related to the plant hormone cytokinin. This compound was synthesized from l-aspartic acid, and its activities were confirmed by three classical cytokinin bioassays as well as by using binding and activation assays with the Arabidopsis cytokinin receptors AHK3 and CRE1/AHK4.
[Mh] Termos MeSH primário: Adenina/análogos & derivados
Arabidopsis/metabolismo
Dictyostelium/química
[Mh] Termos MeSH secundário: Adenina/síntese química
Adenina/química
Adenina/farmacologia
Ácido Aspártico/química
Citocininas/química
Citocininas/metabolismo
Estrutura Molecular
Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokinins); 30KYC7MIAI (Aspartic Acid); 62061-49-8 (discadenine); JAC85A2161 (Adenine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170701
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.6b01165


  7 / 2032 MEDLINE  
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[PMID]:28594957
[Au] Autor:Maniga A; Ghisaura S; Perrotta L; Marche MG; Cella R; Albani D
[Ad] Endereço:Department of Agriculture, University of Sassari, Sassari, Italy.
[Ti] Título:Distinctive features and differential regulation of the DRTS genes of Arabidopsis thaliana.
[So] Source:PLoS One;12(6):e0179338, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In plants and protists, dihydrofolate reductase (DHFR) and thymidylate synthase (TS) are part of a bifunctional enzyme (DRTS) that allows efficient recycling of the dihydrofolate resulting from TS activity. Arabidopsis thaliana possesses three DRTS genes, called AtDRTS1, AtDRTS2 and AtDRTS3, that are located downstream of three members of the sec14-like SFH gene family. In this study, a characterization of the AtDRTS genes identified alternatively spliced transcripts coding for AtDRTS isoforms which may account for monofunctional DHFR enzymes supporting pathways unrelated to DNA synthesis. Moreover, we discovered a complex differential regulation of the AtDRTS genes that confirms the expected involvement of the AtDRTS genes in cell proliferation and endoreduplication, but indicates also functions related to other cellular activities. AtDRTS1 is widely expressed in both meristematic and differentiated tissues, whereas AtDRTS2 expression is almost exclusively limited to the apical meristems and AtDRTS3 is preferentially expressed in the shoot apex, in stipules and in root cap cells. The differential regulation of the AtDRTS genes is associated to distinctive promoter architectures and the expression of AtDRTS1 in the apical meristems is strictly dependent on the presence of an intragenic region that includes the second intron of the gene. Upon activation of cell proliferation in germinating seeds, the activity of the AtDRTS1 and AtDRTS2 promoters in meristematic cells appears to be maximal at the G1/S phase of the cell cycle. In addition, the promoters of AtDRTS2 and AtDRTS3 are negatively regulated through E2F cis-acting elements and both genes, but not AtDRTS1, are downregulated in plants overexpressing the AtE2Fa factor. Our study provides new information concerning the function and the regulation of plant DRTS genes and opens the way to further investigations addressing the importance of folate synthesis with respect to specific cellular activities.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/genética
Arabidopsis/enzimologia
Arabidopsis/genética
Regulação da Expressão Gênica de Plantas
Genes de Plantas
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Arabidopsis/citologia
Arabidopsis/efeitos dos fármacos
Proteínas de Arabidopsis/química
Proteínas de Arabidopsis/metabolismo
Ciclo Celular/efeitos dos fármacos
Ciclo Celular/genética
Proliferação Celular/efeitos dos fármacos
Proliferação Celular/genética
Simulação por Computador
Citocininas/farmacologia
Regulação para Baixo/efeitos dos fármacos
Regulação para Baixo/genética
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Germinação/efeitos dos fármacos
Germinação/genética
Glucuronidase/metabolismo
Ácidos Indolacéticos/farmacologia
Íntrons/genética
Isoenzimas/química
Isoenzimas/genética
Isoenzimas/metabolismo
Meristema/efeitos dos fármacos
Meristema/genética
Fases de Leitura Aberta/genética
Plantas Geneticamente Modificadas
Regiões Promotoras Genéticas
Sementes/efeitos dos fármacos
Sementes/genética
Sementes/crescimento & desenvolvimento
Frações Subcelulares/efeitos dos fármacos
Frações Subcelulares/enzimologia
Transformação Genética/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Cytokinins); 0 (Indoleacetic Acids); 0 (Isoenzymes); EC 3.2.1.31 (Glucuronidase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170609
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179338


  8 / 2032 MEDLINE  
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[PMID]:28426669
[Au] Autor:García-Gómez ML; Azpeitia E; Álvarez-Buylla ER
[Ad] Endereço:Departamento de Ecología Funcional, Instituto de Ecología, Universidad Nacional Autónoma de México, Coyoacán, Ciudad de México, México.
[Ti] Título:A dynamic genetic-hormonal regulatory network model explains multiple cellular behaviors of the root apical meristem of Arabidopsis thaliana.
[So] Source:PLoS Comput Biol;13(4):e1005488, 2017 Apr.
[Is] ISSN:1553-7358
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The study of the concerted action of hormones and transcription factors is fundamental to understand cell differentiation and pattern formation during organ development. The root apical meristem of Arabidopsis thaliana is a useful model to address this. It has a stem cell niche near its tip conformed of a quiescent organizer and stem or initial cells around it, then a proliferation domain followed by a transition domain, where cells diminish division rate before transiting to the elongation zone; here, cells grow anisotropically prior to their final differentiation towards the plant base. A minimal model of the gene regulatory network that underlies cell-fate specification and patterning at the root stem cell niche was proposed before. In this study, we update and couple such network with both the auxin and cytokinin hormone signaling pathways to address how they collectively give rise to attractors that correspond to the genetic and hormonal activity profiles that are characteristic of different cell types along A. thaliana root apical meristem. We used a Boolean model of the genetic-hormonal regulatory network to integrate known and predicted regulatory interactions into alternative models. Our analyses show that, after adding some putative missing interactions, the model includes the necessary and sufficient components and regulatory interactions to recover attractors characteristic of the root cell types, including the auxin and cytokinin activity profiles that correlate with different cellular behaviors along the root apical meristem. Furthermore, the model predicts the existence of activity configurations that could correspond to the transition domain. The model also provides a possible explanation for apparently paradoxical cellular behaviors in the root meristem. For example, how auxin may induce and at the same time inhibit WOX5 expression. According to the model proposed here the hormonal regulation of WOX5 might depend on the cell type. Our results illustrate how non-linear multi-stable qualitative network models can aid at understanding how transcriptional regulators and hormonal signaling pathways are dynamically coupled and may underlie both the acquisition of cell fate and the emergence of hormonal activity profiles that arise during complex organ development.
[Mh] Termos MeSH primário: Arabidopsis/crescimento & desenvolvimento
Arabidopsis/genética
Regulação da Expressão Gênica de Plantas/genética
Redes Reguladoras de Genes/genética
Meristema/crescimento & desenvolvimento
Meristema/genética
[Mh] Termos MeSH secundário: Arabidopsis/metabolismo
Proteínas de Arabidopsis/genética
Proteínas de Arabidopsis/metabolismo
Diferenciação Celular/genética
Diferenciação Celular/fisiologia
Biologia Computacional
Citocininas/metabolismo
Ácidos Indolacéticos/metabolismo
Meristema/citologia
Meristema/metabolismo
Modelos Biológicos
Transdução de Sinais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Cytokinins); 0 (Indoleacetic Acids)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170421
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pcbi.1005488


  9 / 2032 MEDLINE  
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[PMID]:28419168
[Au] Autor:Yamburenko MV; Kieber JJ; Schaller GE
[Ad] Endereço:Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire, United States of America.
[Ti] Título:Dynamic patterns of expression for genes regulating cytokinin metabolism and signaling during rice inflorescence development.
[So] Source:PLoS One;12(4):e0176060, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Inflorescence development in cereals, including such important crops as rice, maize, and wheat, directly affects grain number and size and is a key determinant of yield. Cytokinin regulates meristem size and activity and, as a result, has profound effects on inflorescence development and architecture. To clarify the role of cytokinin action in inflorescence development, we used the NanoString nCounter system to analyze gene expression in the early stages of rice panicle development, focusing on 67 genes involved in cytokinin biosynthesis, degradation, and signaling. Results point toward key members of these gene families involved in panicle development and indicate that the expression of many genes involved in cytokinin action differs between the panicle and vegetative tissues. Dynamic patterns of gene expression suggest that subnetworks mediate cytokinin action during different stages of panicle development. The variation of expression during panicle development is greater among genes encoding proteins involved in cytokinin metabolism and negative regulators of the pathway than for the genes in the primary response pathway. These results provide insight into the expression patterns of genes involved in cytokinin action during inflorescence development in a crop of agricultural importance, with relevance to similar processes in other monocots. The identification of subnetworks of genes expressed at different stages of early panicle development suggests that manipulation of their expression could have substantial effects on inflorescence architecture.
[Mh] Termos MeSH primário: Citocininas/metabolismo
Regulação da Expressão Gênica de Plantas
Inflorescência/crescimento & desenvolvimento
Oryza/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Produtos Agrícolas/genética
Produtos Agrícolas/crescimento & desenvolvimento
Produtos Agrícolas/metabolismo
Citocininas/genética
Regulação da Expressão Gênica no Desenvolvimento
Genes de Plantas
Inflorescência/genética
Inflorescência/metabolismo
Redes e Vias Metabólicas
Família Multigênica
Oryza/genética
Oryza/metabolismo
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokinins); 0 (Plant Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170505
[Lr] Data última revisão:
170505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170419
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0176060


  10 / 2032 MEDLINE  
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[PMID]:28388635
[Au] Autor:Reyes-Olalde JI; Zúñiga-Mayo VM; Serwatowska J; Chavez Montes RA; Lozano-Sotomayor P; Herrera-Ubaldo H; Gonzalez-Aguilera KL; Ballester P; Ripoll JJ; Ezquer I; Paolo D; Heyl A; Colombo L; Yanofsky MF; Ferrandiz C; Marsch-Martínez N; de Folter S
[Ad] Endereço:Unidad de Genómica Avanzada (LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Irapuato, Guanajuato, México.
[Ti] Título:The bHLH transcription factor SPATULA enables cytokinin signaling, and both activate auxin biosynthesis and transport genes at the medial domain of the gynoecium.
[So] Source:PLoS Genet;13(4):e1006726, 2017 Apr.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fruits and seeds are the major food source on earth. Both derive from the gynoecium and, therefore, it is crucial to understand the mechanisms that guide the development of this organ of angiosperm species. In Arabidopsis, the gynoecium is composed of two congenitally fused carpels, where two domains: medial and lateral, can be distinguished. The medial domain includes the carpel margin meristem (CMM) that is key for the production of the internal tissues involved in fertilization, such as septum, ovules, and transmitting tract. Interestingly, the medial domain shows a high cytokinin signaling output, in contrast to the lateral domain, where it is hardly detected. While it is known that cytokinin provides meristematic properties, understanding on the mechanisms that underlie the cytokinin signaling pattern in the young gynoecium is lacking. Moreover, in other tissues, the cytokinin pathway is often connected to the auxin pathway, but we also lack knowledge about these connections in the young gynoecium. Our results reveal that cytokinin signaling, that can provide meristematic properties required for CMM activity and growth, is enabled by the transcription factor SPATULA (SPT) in the medial domain. Meanwhile, cytokinin signaling is confined to the medial domain by the cytokinin response repressor ARABIDOPSIS HISTIDINE PHOSPHOTRANSFERASE 6 (AHP6), and perhaps by ARR16 (a type-A ARR) as well, both present in the lateral domains (presumptive valves) of the developing gynoecia. Moreover, SPT and cytokinin, probably together, promote the expression of the auxin biosynthetic gene TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS 1 (TAA1) and the gene encoding the auxin efflux transporter PIN-FORMED 3 (PIN3), likely creating auxin drainage important for gynoecium growth. This study provides novel insights in the spatiotemporal determination of the cytokinin signaling pattern and its connection to the auxin pathway in the young gynoecium.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/genética
Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética
Citocininas/metabolismo
Meristema/genética
[Mh] Termos MeSH secundário: Arabidopsis/genética
Arabidopsis/crescimento & desenvolvimento
Proteínas de Arabidopsis/metabolismo
Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo
Flores/genética
Flores/crescimento & desenvolvimento
Frutas/genética
Frutas/crescimento & desenvolvimento
Regulação da Expressão Gênica de Plantas
Ácidos Indolacéticos/metabolismo
Meristema/crescimento & desenvolvimento
Sementes/genética
Sementes/crescimento & desenvolvimento
Transdução de Sinais
Triptofano Transaminase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (AHP6 protein, Arabidopsis); 0 (ARR16 protein, Arabidopsis); 0 (Arabidopsis Proteins); 0 (Basic Helix-Loop-Helix Transcription Factors); 0 (Cytokinins); 0 (Indoleacetic Acids); 0 (PIN3 protein, Arabidopsis); 0 (SPT protein, Arabidopsis); EC 2.6.1.27 (Tryptophan Transaminase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170602
[Lr] Data última revisão:
170602
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170408
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006726



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