Base de dados : MEDLINE
Pesquisa : D03.633.100.759.138.525.400 [Categoria DeCS]
Referências encontradas : 761 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 77 ir para página                         

  1 / 761 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28323427
[Au] Autor:Osgerby L; Lai YC; Thornton PJ; Amalfitano J; Le Duff CS; Jabeen I; Kadri H; Miccoli A; Tucker JHR; Muqit MMK; Mehellou Y
[Ad] Endereço:School of Chemistry, University of Birmingham , Birmingham, B15 2TT, U.K.
[Ti] Título:Kinetin Riboside and Its ProTides Activate the Parkinson's Disease Associated PTEN-Induced Putative Kinase 1 (PINK1) Independent of Mitochondrial Depolarization.
[So] Source:J Med Chem;60(8):3518-3524, 2017 Apr 27.
[Is] ISSN:1520-4804
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Since loss of function mutations of PINK1 lead to early onset Parkinson's disease, there has been growing interest in the discovery of small molecules that amplify the kinase activity of PINK1. We herein report the design, synthesis, serum stability, and hydrolysis of four kinetin riboside ProTides. These ProTides, along with kinetin riboside, activated PINK1 in cells independent of mitochondrial depolarization. This highlights the potential of modified nucleosides and their phosphate prodrugs as treatments for neurodegenerative diseases.
[Mh] Termos MeSH primário: Adenosina/farmacologia
Cinetina/farmacologia
Mitocôndrias/enzimologia
Doença de Parkinson/enzimologia
Proteínas Quinases/metabolismo
[Mh] Termos MeSH secundário: Animais
Espectroscopia de Ressonância Magnética Nuclear de Carbono-13
Seres Humanos
Espectroscopia de Prótons por Ressonância Magnética
Espectrometria de Massas por Ionização por Electrospray
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (kinetin riboside); EC 2.7.- (Protein Kinases); EC 2.7.11.1 (PTEN-induced putative kinase); K72T3FS567 (Adenosine); P39Y9652YJ (Kinetin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170620
[Lr] Data última revisão:
170620
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jmedchem.6b01897


  2 / 761 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28278649
[Au] Autor:Kubica P; Szopa A; Ekiert H
[Ad] Endereço:a Department of Pharmaceutical Botany, Medical College , Jagiellonian University , Kraków , Poland.
[Ti] Título:Production of verbascoside and phenolic acids in biomass of Verbena officinalis L. (vervain) cultured under different in vitro conditions.
[So] Source:Nat Prod Res;31(14):1663-1668, 2017 Jul.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Methanolic extracts from the biomass of Verbena officinalis cultured under continuous artificial light and in darkness on 12 variants of the Murashige and Skoog medium containing different concentrations (0.5-3.0 mg/L) of plant growth regulators: 6-benzyladenine, kinetin, 1-naphthaleneacetic acid and indole-3-butyric acid, were analysed for the amounts of verbascoside and phenolic acids, before and after acid hydrolysis, using the HPLC-DAD method. The amounts of verbascoside were very high (max. 2454.12 mg/100 g DW - light, and 2135.59 mg/100 g DW - darkness). The total amounts of phenolic acids reached a maximum of 46.02 mg/100 g DW (free phenolic acids) and 141.05 mg/100 g DW (bound compounds). The main metabolites were: ferulic, o-coumaric and caffeic acids. The maximum amount of verbascoside was 3.28 times higher than in extracts from the herb of the parent plant. The cultures could be proposed as a potential biotechnological source for selected biologically active compounds.
[Mh] Termos MeSH primário: Biomassa
Hidroxibenzoatos/síntese química
Extratos Vegetais/química
Verbena/química
[Mh] Termos MeSH secundário: Compostos de Benzil
Ácidos Cafeicos/metabolismo
Cromatografia Líquida de Alta Pressão
Glucosídeos
Indóis
Cinetina
Ácidos Naftalenoacéticos
Fenóis
Reguladores de Crescimento de Planta/análise
Purinas
Verbena/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzyl Compounds); 0 (Caffeic Acids); 0 (Glucosides); 0 (Hydroxybenzoates); 0 (Indoles); 0 (Naphthaleneacetic Acids); 0 (Phenols); 0 (Plant Extracts); 0 (Plant Growth Regulators); 0 (Purines); 061SKE27JP (indolebutyric acid); 29656-58-4 (phenolic acid); 33T7G7757C (1-naphthaleneacetic acid); 3TGX09BD5B (acteoside); KXG6A989PS (benzylaminopurine); P39Y9652YJ (Kinetin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2017.1286477


  3 / 761 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28153445
[Au] Autor:Voller J; Béres T; Zatloukal M; Kaminski PA; Niemann P; Dolezal K; Dzubák P; Hajdúch M; Strnad M
[Ad] Endereço:Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany ASCR & Palacký University, Slechtitelu 27, CZ-78371 Olomouc, Czechia. Electronic address: jiri.voller@upol.cz.
[Ti] Título:The natural cytokinin 2OH3MeOBAR induces cell death by a mechanism that is different from that of the "classical" cytokinin ribosides.
[So] Source:Phytochemistry;136:156-164, 2017 Apr.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cytokinin ribosides (N -substituted adenosines) have demonstrated anticancer activity in various cultured cell lines, several xenografts and even a small clinical trial. Effects of kinetin riboside, N -benzyladenosine (BAR) and N -isopentenyladenosine on various parameters related to apoptosis have also been reported, but not directly compared with those of the highly active naturally occurring aromatic cytokinins oTR (ortho-topolin riboside) and 2OH3MeOBAR (N -(2-hydroxy-3-methoxybenzyl)adenosine). Here we show that 2OH3MeOBAR is the most active cytokinin riboside studied to date (median, 1st quartile, 3rd quartile and range of GI50 in tests with the NCI60 cell panel: 0.19, 0.10, 0.43 and 0.02 to 15.7 µM, respectively) and it differs from other cytokinins by inducing cell death without causing pronounced ATP depletion. Analysis of NCI60 test data suggests that its activity is independent of p53 status. Further we demonstrate that its 5'-monophosphate, the dominant cancer cell metabolite, inhibits the candidate oncogene DNPH1. Synthesis, purification, HPLC-MS identification and HPLC-UV quantification of 2OH3MeOBAR metabolites are also reported.
[Mh] Termos MeSH primário: Adenosina/farmacologia
Citocininas/farmacologia
[Mh] Termos MeSH secundário: Adenosina/análogos & derivados
Adenosina/química
Apoptose/efeitos dos fármacos
Morte Celular/efeitos dos fármacos
Citocininas/química
Glicosídeos/farmacologia
Isopenteniladenosina/farmacologia
Cinetina/farmacologia
Estrutura Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokinins); 0 (Glycosides); 0 (N6-(2-hydroxy-3-methoxybenzyl)adenosine); 0 (kinetin riboside); 0 (ortho-topolin riboside); 7724-76-7 (Isopentenyladenosine); K72T3FS567 (Adenosine); P39Y9652YJ (Kinetin)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170321
[Lr] Data última revisão:
170321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170204
[St] Status:MEDLINE


  4 / 761 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27999977
[Au] Autor:Kolachevskaya OO; Sergeeva LI; Floková K; Getman IA; Lomin SN; Alekseeva VV; Rukavtsova EB; Buryanov YI; Romanov GA
[Ad] Endereço:Laboratory of Signaling Systems, Institute of Plant Physiology RAS, Moscow, 127276, Russia.
[Ti] Título:Auxin synthesis gene tms1 driven by tuber-specific promoter alters hormonal status of transgenic potato plants and their responses to exogenous phytohormones.
[So] Source:Plant Cell Rep;36(3):419-435, 2017 Mar.
[Is] ISSN:1432-203X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: Ectopic auxin overproduction in transgenic potato leads to enhanced productivity accompanied with concerted and occasional changes in hormonal status, and causing altered response of transformants to exogenous auxin or cytokinin. Previously, we generated potato transformants expressing Agrobacterium-derived auxin synthesis gene tms1 driven by tuber-specific patatin gene promoter (B33-promoter). Here, we studied the endogenous hormonal status and the response to exogenous phytohormones in tms1 transformants cultured in vitro. Adding indole-3-acetic acid (IAA) or kinetin to culture medium affected differently tuberization of tms1-transformed and control plants, depending also on sucrose content in the medium. Exogenous phytohormones ceased to stimulate the tuber initiation in transformants at high (5-8%) sucrose concentration, while in control plants the stimulation was observed in all experimental settings. Furthermore, exogenous auxin partly inhibited the tuber initiation, and exogenous cytokinin reduced the average tuber weight in most transformants at high sucrose content. The elevated auxin level in tubers of the transformants was accompanied with a decrease in content of cytokinin bases and their ribosides in tubers and most shoots. No concerted changes in contents of abscisic, jasmonic, salicylic acids and gibberellins in tubers were detected. The data on hormonal status indicated that the enhanced productivity of tms1 transformants was due to auxin and not mediated by other phytohormones. In addition, exogenous cytokinin was shown to upregulate the expression of genes encoding orthologs of auxin receptors. Overall, the results showed that tms1 expression and local increase in IAA level in transformants affect both the balance of endogenous cytokinins and the dynamics of tuberization in response to exogenous hormones (auxin, cytokinin), the latter reaction depending also on the carbohydrate supply. We introduce a basic model for the hormonal network controlling tuberization.
[Mh] Termos MeSH primário: Genes de Plantas
Ácidos Indolacéticos/metabolismo
Reguladores de Crescimento de Planta/farmacologia
Proteínas de Plantas/genética
Tubérculos/genética
Regiões Promotoras Genéticas
Solanum tuberosum/genética
[Mh] Termos MeSH secundário: Biomassa
Citocininas/metabolismo
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Cinetina/farmacologia
Especificidade de Órgãos/efeitos dos fármacos
Proteínas de Plantas/metabolismo
Tubérculos/efeitos dos fármacos
Plantas Geneticamente Modificadas
Receptores de Superfície Celular/genética
Receptores de Superfície Celular/metabolismo
Solanum tuberosum/efeitos dos fármacos
Transformação Genética/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokinins); 0 (Indoleacetic Acids); 0 (Plant Growth Regulators); 0 (Plant Proteins); 0 (Receptors, Cell Surface); 0 (auxin receptor, plant); P39Y9652YJ (Kinetin)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161222
[St] Status:MEDLINE
[do] DOI:10.1007/s00299-016-2091-y


  5 / 761 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27979283
[Au] Autor:Ranjitha K; Shivashankara KS; Sudhakar Rao DV; Oberoi HS; Roy TK; Bharathamma H
[Ad] Endereço:Division of Post Harvest Technology and Agricultural Engineering, ICAR-Indian Institute of Horticultural Research, Hessaraghatta, Bengaluru 560089, India. Electronic address: ranjiha@iihr.res.in.
[Ti] Título:Improvement in shelf life of minimally processed cilantro leaves through integration of kinetin pretreatment and packaging interventions: Studies on microbial population dynamics, biochemical characteristics and flavour retention.
[So] Source:Food Chem;221:844-854, 2017 Apr 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Effect of integrating optimized combination of pretreatment with packaging on shelf life of minimally processed cilantro leaves (MPCL) was appraised through analysis of their sensory attributes, biochemical characteristics, microbial population and flavour profile during storage. Minimally pretreated cilantro leaves pretreated with 50ppm kinetin and packed in 25µ polypropylene bags showed a shelf life of 21days. Optimized combination helped in efficiently maintaining sensory parameters, flavour profile, and retention of antioxidants in MPCL until 21days. Studies conducted on the effect of optimized combination on microbial population and flavour profile revealed that among different microorganisms, pectinolysers had a significant effect on spoilage of MPCL and their population of ⩽3.59logcfu/g was found to be acceptable. Principal component analysis of headspace volatiles revealed that (E)-2-undecenal, (E)-2-hexadecenal, (E)-2-tetradecenal & (E)-2-tetradecen-1-ol in stored samples clustered with fresh samples and therefore, could be considered as freshness indicators for MPCL.
[Mh] Termos MeSH primário: Coriandrum/microbiologia
Embalagem de Alimentos
Conservação de Alimentos
Cinetina/química
Folhas de Planta/microbiologia
[Mh] Termos MeSH secundário: Contagem de Colônia Microbiana
Comportamento do Consumidor
Relação Dose-Resposta a Droga
Contaminação de Alimentos/prevenção & controle
Microbiologia de Alimentos
Qualidade dos Alimentos
Seres Humanos
Paladar
Compostos Orgânicos Voláteis/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Volatile Organic Compounds); P39Y9652YJ (Kinetin)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161217
[St] Status:MEDLINE


  6 / 761 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27942841
[Au] Autor:Kazmierczak A; Doniak M; Bernat P
[Ad] Endereço:Department of Cytophysiology, Faculty of Biology and Environmental Protection The University of Lódz, Pomorska 141/143, 90-236, Lódz, Poland. kazand@biol.uni.lodz.pl.
[Ti] Título:Membrane-related hallmarks of kinetin-induced PCD of root cortex cells.
[So] Source:Plant Cell Rep;36(2):343-353, 2017 Feb.
[Is] ISSN:1432-203X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: Changes in cellular membrane potential and their fluidisation are the hallmarks of cell death induction with kinetin in root cortex. Programmed cell death (PCD), one of the essential processes in plant development, is still poorly understood. In this paper, the scientific plant model, V. faba ssp. minor seedling roots after kinetin application which triggers off programmed death of cortex cells, was used to recognise membrane-related aspects of plant cell death. Spectrophotometric, reflectometric and microscopic studies showed that the PCD induced by kinetin is accompanied by higher potassium ions leakage from roots, loss of plasma and ER membrane potentials (expressed by their lower amounts and higher index of fatty acid unsaturation), malformation of nuclear envelope, lower total lipid amount and formation of their peroxides, lower amount of phospholipids and changes in their composition. The results showed that potassium ions leakage, expressed in percentage of their amounts, and loss of plasma and ER membrane potential, expressed in percentage of their fluorescence intensity, together with the nuclear chromatin double staining with ethidium bromide and acridine orange, might be direct and universal methods for detecting specific plant PCD hallmarks and estimation of PCD intensity (percentage of dying and dead cells).
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Membrana Celular/metabolismo
Cinetina/farmacologia
Células Vegetais/metabolismo
Raízes de Plantas/citologia
[Mh] Termos MeSH secundário: Membrana Celular/efeitos dos fármacos
Condutividade Elétrica
Ácidos Graxos/metabolismo
Fluorescência
Fosfolipídeos/química
Células Vegetais/efeitos dos fármacos
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids); 0 (Phospholipids); P39Y9652YJ (Kinetin)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE
[do] DOI:10.1007/s00299-016-2085-9


  7 / 761 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27542499
[Au] Autor:Nashalian O; Yaylayan VA
[Ad] Endereço:Department of Food Science and Agricultural Chemistry, McGill University, 21,111 Lakeshore, Ste. Anne de Bellevue, Quebec H9X 3V9, Canada.
[Ti] Título:Reactivity of nitrogen atoms in adenine and (Ade)2Cu complexes towards ribose and 2-furanmethanol: Formation of adenosine and kinetin.
[So] Source:Food Chem;215:463-9, 2017 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:To explore the interaction of nucleosides and nucleobases in the context of the Maillard reaction and to identify the selectivity of purine nitrogen atoms towards various electrophiles, model systems composed of adenine or adenosine, glycine, ribose and/or 2-furanmethanol (with and without copper) were studied in aqueous solutions heated at 110°C for 2h and subsequently analyzed by ESI/qTOF/MS/MS in addition to isotope labelling techniques. The results indicated that ribose selectively formed mono-ribosylated N(6) adenine, but in the presence of (Ade)2Cu complex the reaction mixture generated mono-, di- and tri-substituted sugar complexes and their hydrolysis products of mono-ribosylated N(6) and N(9) adenine adducts and di-ribosylated N(6,9) adenine. Furthermore, the reaction of 2-furanmethanol with adenine in the presence of ribose generated kinetin and its isomer, while its reaction with adenosine generated kinetin riboside, as confirmed by comparing the MS/MS profiles of these adducts to those of commercial standards.
[Mh] Termos MeSH primário: Adenina/química
Adenosina/química
Cinetina/química
Reação de Maillard
Nitrogênio/química
Ribose/química
[Mh] Termos MeSH secundário: Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
681HV46001 (Ribose); JAC85A2161 (Adenine); K72T3FS567 (Adenosine); N762921K75 (Nitrogen); P39Y9652YJ (Kinetin)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160821
[St] Status:MEDLINE


  8 / 761 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27511192
[Au] Autor:Cheng WH; Zhu HG; Tian WG; Zhu SH; Xiong XP; Sun YQ; Zhu QH; Sun J
[Ad] Endereço:The Key Laboratory of Oasis Eco-Agriculture, College of Agriculture, Shihezi University, Shihezi, 832000, Xinjiang, China.
[Ti] Título:De novo transcriptome analysis reveals insights into dynamic homeostasis regulation of somatic embryogenesis in upland cotton (G. hirsutum L.).
[So] Source:Plant Mol Biol;92(3):279-92, 2016 Oct.
[Is] ISSN:1573-5028
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Plant regeneration via somatic embryogenesis (SE) is the key step for genetic improvement of cotton (Gossypium hirsutum L.) through genetic engineering mediated by Agrobacteria, but the molecular mechanisms underlying SE in cotton is still unclear. Here, RNA-Sequencing was used to analyze the genes expressed during SE and their expression dynamics using RNAs isolated from non-embryogenic callus (NEC), embryogenic callus (EC) and somatic embryos (SEs). A total of 101, 670 unigenes were de novo assembled. The genes differentially expressed (DEGs) amongst NEC, EC and SEs were identified, annotated and classified. More DEGs were found between SEs and EC than between EC and NEC. A significant number of DEGs were related to hormone homeostasis, stress and ROS responses, and metabolism of polyamines. To confirm the expression dynamics of selected DEGs involved in various pathways, experiments were set up to investigate the effects of hormones (Indole-3-butytric acid, IBA; Kinetin, KT), polyamines, H2O2 and stresses on SE. Our results showed that exogenous application of IBA and KT positively regulated the development of EC and SEs, and that polyamines and H2O2 promoted the conversion of EC into SEs. Furthermore, we found that low and moderate stress is beneficial for proliferation of EC and SEs formation. Together, our global analysis of transcriptomic dynamics reveals that hormone homeostasis, polyamines, and stress response synergistically regulating SE in cotton.
[Mh] Termos MeSH primário: Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Gossypium/genética
Homeostase/genética
[Mh] Termos MeSH secundário: Gossypium/embriologia
Cinetina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
P39Y9652YJ (Kinetin)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170224
[Lr] Data última revisão:
170224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160812
[St] Status:MEDLINE
[do] DOI:10.1007/s11103-016-0511-6


  9 / 761 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27459816
[Au] Autor:Liu P; Fan S; Wu G; Zhao R; Liu W; Zhao X
[Ti] Título:[Determination of 6 kinds of plant growth regulator in bean sprout by ultra high performance liquid chromatography-tandem mass spectrometry].
[So] Source:Wei Sheng Yan Jiu;45(3):483-9, 2016 May.
[Is] ISSN:1000-8020
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: A method for the simultaneous determination of 6 plant growth regulator (PGR) residues in bean sprout was developed by ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). 6-Benzylaminopurine, isopentennyladenine, 4-chlorophenoxyacetic acid, 4-fluorophenoxyacetic acid, indole-3- acetic acid and indole-3-butyric acid were concerned. METHODS: Bean sprout samples were extracted by acetonitrile and QuEChERS extraction kit, purified by C18 powers. After centrifugation, the sample liquids was diluted 10 times by ultrapure water. The chromatographic analysis was carried out on an waters acquity UPLC BEH C18 column( 100 mm x 2.1 mm, 1.7 microm). The analyzer confirmed and quantified by mass spectrum of triple quadrupole in the multiple reaction monitoring (MRM) mode and quantified by matrix-matched external standard method. RESULTS: The calibration curves showed good linearity in each range with correlation coefficients greater than 0.998. 3 levels spiked recoveries were carried out using blank bean sprout extraction as substrate, the recoveries ranged from 84.2% to 107.5%, the relative standard deviations (RSDs) ranged from 3.08% to 12.71%. The qualitative limits of detections (S/N = 3) were 0.03-3.0 microg/kg and the quantitative limits(S/N = 10) were 0.1-10.0 microg/kg for the 6 PGRs. CONSLUSION: The method is simple and easy to operate, with less organic reagent, high sensitivity and good stability. It is suitable for the detection of 6 kinds of plant growth regulators in bean sprouts.
[Mh] Termos MeSH primário: Phaseolus/química
Reguladores de Crescimento de Planta/análise
[Mh] Termos MeSH secundário: Ácido 2,4-Diclorofenoxiacético/análogos & derivados
Ácido 2,4-Diclorofenoxiacético/análise
Compostos de Benzil
Cromatografia Líquida de Alta Pressão
Ácidos Indolacéticos/análise
Indóis/análise
Isopenteniladenosina/análise
Cinetina/análise
Purinas
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzyl Compounds); 0 (Indoleacetic Acids); 0 (Indoles); 0 (Plant Growth Regulators); 0 (Purines); 061SKE27JP (indolebutyric acid); 2365-40-4 (N(6)-(delta(2)-isopentenyl)adenine); 2577AQ9262 (2,4-Dichlorophenoxyacetic Acid); 4EMM3U5P3K (4-chlorophenoxyacetic acid); 6U1S09C61L (indoleacetic acid); 7724-76-7 (Isopentenyladenosine); KXG6A989PS (benzylaminopurine); P39Y9652YJ (Kinetin)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160728
[St] Status:MEDLINE


  10 / 761 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27428370
[Au] Autor:Eser A; Aydemir T
[Ad] Endereço:Celal Bayar University, Faculty of Science and Letters, Department of Chemistry, 45140, Manisa Turkey.
[Ti] Título:The effect of kinetin on wheat seedlings exposed to boron.
[So] Source:Plant Physiol Biochem;108:158-164, 2016 Nov.
[Is] ISSN:1873-2690
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:The objective of this study was to examine relationship between boron (B) induced oxidative stress and antioxidant system in boron sensitive and tolerant wheat cultivars Bezostaya and Kutluk, and also to investigate whether Kinetin (KN) enhances the level of antioxidant system, relative growth, concentration of hydrogen peroxide (H O ), malondialdehyde (MDA) and proline and chlorophyll content in both cultivars exposed to B stress. B treatments diminished growth and chlorophyll content whereas, it enhanced accumulation of H O , MDA and proline, and various antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX) and lipoxygenase (LOX) in the shoot and root of both cultivars. However, the follow-up application of KN to the B stressed plants improved growth and chlorophyll content and further enhanced the mentioned antioxidant enzymes and level of H O , MDA and proline. This study thus suggests that KN improves B tolerance of the studied cultivars grown under B toxicity.
[Mh] Termos MeSH primário: Boro/toxicidade
Cinetina/farmacologia
Plântulas/efeitos dos fármacos
Triticum/efeitos dos fármacos
[Mh] Termos MeSH secundário: Antioxidantes/metabolismo
Ascorbato Peroxidases/metabolismo
Boro/farmacocinética
Catalase/metabolismo
Peróxido de Hidrogênio/metabolismo
Lipoxigenase/metabolismo
Malondialdeído/metabolismo
Estresse Oxidativo/efeitos dos fármacos
Peroxidase/metabolismo
Proteínas de Plantas/metabolismo
Prolina/metabolismo
Plântulas/crescimento & desenvolvimento
Plântulas/metabolismo
Superóxido Dismutase/metabolismo
Distribuição Tecidual
Triticum/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Plant Proteins); 4Y8F71G49Q (Malondialdehyde); 9DLQ4CIU6V (Proline); BBX060AN9V (Hydrogen Peroxide); EC 1.11.1.- (guaiacol peroxidase); EC 1.11.1.11 (Ascorbate Peroxidases); EC 1.11.1.6 (Catalase); EC 1.11.1.7 (Peroxidase); EC 1.13.11.12 (Lipoxygenase); EC 1.15.1.1 (Superoxide Dismutase); N9E3X5056Q (Boron); P39Y9652YJ (Kinetin)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170813
[Lr] Data última revisão:
170813
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160719
[St] Status:MEDLINE



página 1 de 77 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde