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Pesquisa : D03.633.100.759.138.525.700 [Categoria DeCS]
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[PMID]:28737742
[Au] Autor:Osugi A; Kojima M; Takebayashi Y; Ueda N; Kiba T; Sakakibara H
[Ad] Endereço:RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro, Tsurumi, Yokohama 230-0045, Japan.
[Ti] Título:Systemic transport of trans-zeatin and its precursor have differing roles in Arabidopsis shoots.
[So] Source:Nat Plants;3:17112, 2017 Jul 24.
[Is] ISSN:2055-0278
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Organ-to-organ signal transmission is essential for higher organisms to ensure coordinated biological reactions during metabolism and morphogenesis. Similar to organs in animals, plant organs communicate by various signalling molecules. Among them, cytokinins, a class of phytohormones, play a key role as root-to-shoot long-distance signals, regulating various growth and developmental processes in shoots . Previous studies have proposed that trans-zeatin-riboside, a type of cytokinin precursor, is a major long-distance signalling form in xylem vessels and its action depends on metabolic conversion via the LONELY GUY enzyme in proximity to the site of action . Here we report an additional long-distance signalling form of cytokinin: trans-zeatin, an active form. Grafting between various cytokinin biosynthetic and transportation mutants revealed that root-to-shoot translocation of trans-zeatin, a minor component of xylem cytokinin, controls leaf size but not meristem activity-related traits, whereas that of trans-zeatin riboside is sufficient for regulating both traits. Considering the ratio of trans-zeatin to trans-zeatin-riboside in xylem and their delivery rate change in response to environmental conditions, this dual long-distance cytokinin signalling system allows plants to fine-tune the manner of shoot growth to adapt to fluctuating environments.
[Mh] Termos MeSH primário: Arabidopsis/metabolismo
Isopenteniladenosina/análogos & derivados
Brotos de Planta/metabolismo
Zeatina/metabolismo
[Mh] Termos MeSH secundário: Citocininas/metabolismo
Isopenteniladenosina/metabolismo
Transdução de Sinais
Xilema/metabolismo
Zeatina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokinins); 7724-76-7 (Isopentenyladenosine); 7I6OOJ9GR6 (Zeatin); 7LG4D082A9 (zeatin riboside)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170725
[St] Status:MEDLINE
[do] DOI:10.1038/nplants.2017.112


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[PMID]:28061413
[Au] Autor:Zhang X; Yang X; Wang H; Li Q; Wang H; Li Y
[Ad] Endereço:Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, Yunnan Province 650500, China.
[Ti] Título:A significant positive correlation between endogenous trans-zeatin content and total arsenic in arsenic hyperaccumulator Pteris cretica var. nervosa.
[So] Source:Ecotoxicol Environ Saf;138:199-205, 2017 Apr.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A pot experiment was conducted to compare the content of endogenous trans-zeatin (Z), plant arsenic (As) uptake and physiological indices in the fronds of As-hyperaccumulator (Pteris cretica var. nervosa) and non-hyperaccumulator (Pteris ensiformis). Furthermore, a stepwise regression method was used to study the relationship among determined indices, and the time-course effect of main indices was also investigated under 100mg/kg As stress with time extension. In the 100-200mg/kg As treatments, plant height showed no significant difference and endogenous Z content significantly increased in P. cretica var. nervosa compared to the control, but a significant decrease of height and endogenous Z was observed in P. ensiformis. The concentrations of As (III) and As (V) increased significantly in the fronds of two plants, but this increase was much higher in P. cretica var. nervosa. Compared to the control, the contents of chlorophyll and soluble protein were significantly increased in P. cretica var. nervosa but decreased in P. ensiformis in the 200mg/kg As treatment, respectively. A significant positive correlation was found between the contents of endogenous Z and total As in P. cretica var. nervosa, but such a correlation was not found in P. ensiformis. Additionally, in the time-course effect experiment, a peak value of each index was appeared in the 43rd day in two plants, except for chlorophyll in P. ensiformis, but this value was significantly higher in P. cretica var. nervosa than that in P. ensiformis. In conclusion, a higher endogenous Z content contributed to As accumulation of P. cretica var. nervosa under As stress.
[Mh] Termos MeSH primário: Arsênico/metabolismo
Arsênico/farmacologia
Pteris/metabolismo
Poluentes do Solo/metabolismo
Poluentes do Solo/farmacologia
Zeatina/metabolismo
[Mh] Termos MeSH secundário: Arsênico/análise
Biodegradação Ambiental
Clorofila/metabolismo
Pteris/química
Pteris/crescimento & desenvolvimento
Fatores de Tempo
Zeatina/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil Pollutants); 1406-65-1 (Chlorophyll); 7I6OOJ9GR6 (Zeatin); N712M78A8G (Arsenic)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170107
[St] Status:MEDLINE


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[PMID]:27865843
[Au] Autor:Feng Y; Zhang X; Wu T; Xu X; Han Z; Wang Y
[Ad] Endereço:Institute for Horticultural Plants, College of Horticulture, China Agricultural University, Beijing, 100193, China; Key Laboratory of Stress Physiology and Molecular Biology for Fruit Trees in Beijing Municipality, China Agricultural University, Beijing, 100193, China; Key Laboratory of Biology and
[Ti] Título:Methylation effect on IPT5b gene expression determines cytokinin biosynthesis in apple rootstock.
[So] Source:Biochem Biophys Res Commun;482(4):604-609, 2017 Jan 22.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The molecular mechanism of dwarfing in the apple rootstock is poorly understood, and has been attributed to low root cytokinin biosynthesis levels. Here we identified differences in root trans-zeatin content and expression of cytokinin metabolic pathway genes between dwarfing and vigorous rootstocks. Specifically, a stable low expression of IPT5b was identified in the dwarfing rootstocks. Bisulfite sequencing showed that two CpG islands (CpG2 and CpG4) in the IPT5b promoter region showed higher methylation levels in the M9 rootstock (dwarfing) compared to the Mr (vigorous) rootstock. Furthermore, IPT5b expression increased when M9 rootstocks were treated with 5-azaC, a methylation blocker, indicating that methylation levels influence IPT5b expression. In conclusion, we found low IPT5b expression with high level methylations in promoter region, leading to poor root trans-zeatin biosynthesis in the M9 rootstock, which may induce dwarfing.
[Mh] Termos MeSH primário: Alquil e Aril Transferases/genética
Citocininas/genética
Metilação de DNA
Regulação da Expressão Gênica de Plantas
Malus/genética
Proteínas de Plantas/genética
Raízes de Plantas/genética
[Mh] Termos MeSH secundário: Alquil e Aril Transferases/metabolismo
Vias Biossintéticas
Ilhas de CpG
Citocininas/metabolismo
Malus/crescimento & desenvolvimento
Malus/metabolismo
Modelos Moleculares
Filogenia
Proteínas de Plantas/metabolismo
Raízes de Plantas/crescimento & desenvolvimento
Raízes de Plantas/metabolismo
Zeatina/genética
Zeatina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokinins); 0 (Plant Proteins); 7I6OOJ9GR6 (Zeatin); EC 2.5.- (Alkyl and Aryl Transferases); EC 2.5.1.27 (adenylate isopentenyltransferase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170529
[Lr] Data última revisão:
170529
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161121
[St] Status:MEDLINE


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[PMID]:27780273
[Au] Autor:Zhang W; Cao Z; Zhou Q; Chen J; Xu G; Gu J; Liu L; Wang Z; Yang J; Zhang H
[Ad] Endereço:Jiangsu Key Laboratory of Crop Genetics and Physiology, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, China.
[Ti] Título:Grain Filling Characteristics and Their Relations with Endogenous Hormones in Large- and Small-Grain Mutants of Rice.
[So] Source:PLoS One;11(10):e0165321, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study determined if the variation in grain filling parameters between two different spikelet types of rice (Oryza sativa L.) is regulated by the hormonal levels in the grains. Two rice mutants, namely, a large-grain mutant (AZU-M) and a small-grain mutant (ZF802-M), and their respective wild types (AZU-WT and ZF802-WT) were grown in the field. The endosperm cell division rate, filling rate, and hormonal levels: zeatin + zeatin riboside (Z+ZR), indo-3-acetic acid (IAA), polyamines (PAs), and abscisic acid (ABA) were determined. The results showed that there was no significant difference between the filling and endosperm cell division rates. These rates were synchronous between the superior and inferior spikelets for both mutants. However, the abovementioned parameters were significantly different between the two spikelet types for the two wild types. The superior spikelets filled faster and their filling rate was higher compared to the inferior ones. Changes in the concentrations of plant hormones were consistent with the observed endosperm cell division rate and the filling rate for both types of spikelets of mutant and wild type plants. Regression analysis showed a significant positive correlation between cell division and filling rates with the concentrations of the investigated hormones. Exogenous chemical application verified the role of ABA, IAA, and PAs in grain filling. The results indicate that poor filling of inferior spikelets in rice occurs primarily due to the reduced hormone concentrations therein, leading to lower division rate of endosperm cells, fewer endosperm cells, slower filling rate, and smaller grain weight.
[Mh] Termos MeSH primário: Oryza/metabolismo
Reguladores de Crescimento de Planta/análise
[Mh] Termos MeSH secundário: Ácido Abscísico/análise
Ácido Abscísico/isolamento & purificação
Grãos Comestíveis/crescimento & desenvolvimento
Grãos Comestíveis/metabolismo
Endosperma/efeitos dos fármacos
Endosperma/metabolismo
Ensaio de Imunoadsorção Enzimática
Mitoguazona/farmacologia
Oryza/crescimento & desenvolvimento
Reguladores de Crescimento de Planta/isolamento & purificação
Plantas Geneticamente Modificadas/crescimento & desenvolvimento
Plantas Geneticamente Modificadas/metabolismo
Poliaminas/análise
Poliaminas/isolamento & purificação
Putrescina/farmacologia
Espermidina/farmacologia
Zeatina/análise
Zeatina/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Growth Regulators); 0 (Polyamines); 72S9A8J5GW (Abscisic Acid); 7I6OOJ9GR6 (Zeatin); OD5Q0L447W (Mitoguazone); U87FK77H25 (Spermidine); V10TVZ52E4 (Putrescine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170614
[Lr] Data última revisão:
170614
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0165321


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[PMID]:27613625
[Au] Autor:Li Q; Yang X; Wang H; Wang H; He S
[Ad] Endereço:Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, Yunnan, 650500, People's Republic of China.
[Ti] Título:Endogenous trans-zeatin content in plants with different metal-accumulating ability: a field survey.
[So] Source:Environ Sci Pollut Res Int;23(23):23422-23435, 2016 Dec.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A field survey was conducted to evaluate soil metal pollution and endogenous trans-zeatin content in the leaves of plants growing at six sites in a metal-polluted area located in Gejiu, Yunnan, China. Five plant species were collected, and the physicochemical properties and concentrations of five metals in the soil were analyzed. The trans-zeatin content in plant leaves was measured by high-performance liquid chromatography. Based on the Nemerow pollution index, the six sites were classified into four levels of pollution (i.e., low, medium, high, and severely high). The degree of soil metal pollution was cadmium (Cd) > arsenic (As) > lead (Pb) > zinc (Zn) > copper (Cu). The leaf trans-zeatin content in Pteris vittata (an arsenic hyperaccumulator) increased significantly by 98.6 % in soil with a severely high level of pollution compared with soil at a low level of pollution. However, in non-hyperaccumulators Bidens pilosa var. radiata and Ageratina adenophora, a significant decrease in leaf trans-zeatin content of 35.6 and 87.6 %, respectively, was observed. The leaf trans-zeatin content in Artemisia argyi also decreased significantly by 73.6 % in high metal-polluted soil compared with that in medium metal-polluted soil. Furthermore, significant correlations were observed between leaf trans-zeatin content in Pteris vittata and As, Pb, and Cd concentrations in the soil; however, either no correlation or a negative one was observed in the other plant species. Therefore, a high content of trans-zeatin in the leaves of Pteris vittata may play an important role in its normal growth and tolerance to metals.
[Mh] Termos MeSH primário: Arsênico/metabolismo
Asteraceae/metabolismo
Metais Pesados/metabolismo
Pteris/metabolismo
Poluentes do Solo/metabolismo
Zeatina/metabolismo
[Mh] Termos MeSH secundário: China
Cromatografia Líquida de Alta Pressão
Folhas de Planta/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Metals, Heavy); 0 (Soil Pollutants); 7I6OOJ9GR6 (Zeatin); N712M78A8G (Arsenic)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160911
[St] Status:MEDLINE


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[PMID]:27157127
[Au] Autor:Muraro D; Larrieu A; Lucas M; Chopard J; Byrne H; Godin C; King J
[Ad] Endereço:Centre for Plant Integrative Biology, University of Nottingham, Loughborough LE12 5RD, UK; The Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, UK; Mathematical Institute, University of Oxford, Oxford OX2 6GG, UK. Electronic address: Daniele.Muraro@maths.ox.ac.uk.
[Ti] Título:A multi-scale model of the interplay between cell signalling and hormone transport in specifying the root meristem of Arabidopsis thaliana.
[So] Source:J Theor Biol;404:182-205, 2016 Sep 07.
[Is] ISSN:1095-8541
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The growth of the root of Arabidopsis thaliana is sustained by the meristem, a region of cell proliferation and differentiation which is located in the root apex and generates cells which move shootwards, expanding rapidly to cause root growth. The balance between cell division and differentiation is maintained via a signalling network, primarily coordinated by the hormones auxin, cytokinin and gibberellin. Since these hormones interact at different levels of spatial organisation, we develop a multi-scale computational model which enables us to study the interplay between these signalling networks and cell-cell communication during the specification of the root meristem. We investigate the responses of our model to hormonal perturbations, validating the results of our simulations against experimental data. Our simulations suggest that one or more additional components are needed to explain the observed expression patterns of a regulator of cytokinin signalling, ARR1, in roots not producing gibberellin. By searching for novel network components, we identify two mutant lines that affect significantly both root length and meristem size, one of which also differentially expresses a central component of the interaction network (SHY2). More generally, our study demonstrates how a multi-scale investigation can provide valuable insight into the spatio-temporal dynamics of signalling networks in biological tissues.
[Mh] Termos MeSH primário: Arabidopsis/metabolismo
Meristema/citologia
Meristema/metabolismo
Modelos Biológicos
Reguladores de Crescimento de Planta/metabolismo
Transdução de Sinais
[Mh] Termos MeSH secundário: Arabidopsis/efeitos dos fármacos
Proteínas de Arabidopsis/metabolismo
Transporte Biológico/efeitos dos fármacos
Giberelinas/metabolismo
Ácidos Indolacéticos/metabolismo
Meristema/efeitos dos fármacos
Tamanho do Órgão/efeitos dos fármacos
Reprodutibilidade dos Testes
Transdução de Sinais/efeitos dos fármacos
Triazóis/farmacologia
Zeatina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Gibberellins); 0 (Indoleacetic Acids); 0 (Plant Growth Regulators); 0 (Triazoles); 6PLV42R3ZA (paclobutrazol); 7I6OOJ9GR6 (Zeatin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160510
[St] Status:MEDLINE


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[PMID]:27095406
[Au] Autor:Hluska T; Dobrev PI; Tarkowská D; Frébortová J; Zalabák D; Kopecný D; Plíhal O; Kokás F; Briozzo P; Zatloukal M; Motyka V; Galuszka P
[Ad] Endereço:Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University in Olomouc, Slechtitelu 27, 783 71 Olomouc, Czech Republic.
[Ti] Título:Cytokinin metabolism in maize: Novel evidence of cytokinin abundance, interconversions and formation of a new trans-zeatin metabolic product with a weak anticytokinin activity.
[So] Source:Plant Sci;247:127-37, 2016 Jun.
[Is] ISSN:1873-2259
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Cytokinins (CKs) are an important group of phytohormones. Their tightly regulated and balanced levels are essential for proper cell division and plant organ development. Here we report precise quantification of CK metabolites and other phytohormones in maize reproductive organs in the course of pollination and kernel maturation. A novel enzymatic activity dependent on NADP(+) converting trans-zeatin (tZ) to 6-(3-methylpyrrol-1-yl)purine (MPP) was detected. MPP shows weak anticytokinin properties and inhibition of CK dehydrogenases due to their ability to bind to an active site in the opposite orientation than substrates. Although the physiological significance of tZ side-chain cyclization is not anticipated as the MPP occurrence in maize tissue is very low, properties of the novel CK metabolite indicate its potential for utilization in plant in vitro tissue culture. Furthermore, feeding experiments with different isoprenoid CKs revealed distinct preferences in glycosylation of tZ and cis-zeatin (cZ). While tZ is preferentially glucosylated at the N9 position, cZ forms mainly O-glucosides. Since O-glucosides, in contrast to N9-glucosides, are resistant to irreversible cleavage catalyzed by CK dehydrogenases, the observed preference of maize CK glycosyltransferases to O-glycosylate zeatin in the cis-position might be a reason why cZ derivatives are over-accumulated in different maize tissues and organs.
[Mh] Termos MeSH primário: Citocininas/metabolismo
Reguladores de Crescimento de Planta/metabolismo
Terpenos/metabolismo
Zea mays/metabolismo
[Mh] Termos MeSH secundário: Citocininas/análise
Citocininas/isolamento & purificação
Regulação da Expressão Gênica de Plantas
Glicosilação
Glicosiltransferases/metabolismo
Oxirredutases/metabolismo
Reguladores de Crescimento de Planta/análise
Reguladores de Crescimento de Planta/isolamento & purificação
Proteínas de Plantas/metabolismo
Polinização
Plântulas/crescimento & desenvolvimento
Plântulas/metabolismo
Sementes/crescimento & desenvolvimento
Sementes/metabolismo
Terpenos/análise
Terpenos/isolamento & purificação
Zea mays/crescimento & desenvolvimento
Zeatina/análise
Zeatina/isolamento & purificação
Zeatina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cytokinins); 0 (Plant Growth Regulators); 0 (Plant Proteins); 0 (Terpenes); 7I6OOJ9GR6 (Zeatin); EC 1.- (Oxidoreductases); EC 2.4.- (Glycosyltransferases)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160421
[St] Status:MEDLINE


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[PMID]:26661753
[Au] Autor:Roche J; Love J; Guo Q; Song J; Cao M; Fraser K; Huege J; Jones C; Novák O; Turnbull MH; Jameson PE
[Ad] Endereço:School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch, New Zealand.
[Ti] Título:Metabolic changes and associated cytokinin signals in response to nitrate assimilation in roots and shoots of Lolium perenne.
[So] Source:Physiol Plant;156(4):497-511, 2016 Apr.
[Is] ISSN:1399-3054
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Ab] Resumo:The efficiency of inorganic nitrogen (N) assimilation is a critical component of fertilizer use by plants and of forage production in Lolium perenne, an important pasture species worldwide. We present a spatiotemporal description of nitrate use efficiency in terms of metabolic responses and carbohydrate remobilization, together with components of cytokinin signal transduction following nitrate addition to N-impoverished plants. Perennial ryegrass (L. perenne cv. Grasslands Nui) plants were grown for 10 weeks in unfertilized soil and then treated with nitrate (5 mM) hydroponically. Metabolomic analysis by gas chromatography-mass spectrometry and liquid chromatography-tandem mass spectrometry revealed a dynamic interaction between N and carbon metabolism over a week-long time course represented by the relative abundance of amino acids, tricarboxylic acid intermediates and stored water-soluble carbohydrates (WSCs). The initial response to N addition was characterized by a rapid remobilization of carbon stores from the low-molecular weight WSC, along with an increase in N content and assimilation into free amino acids. Subsequently, the shoot became the main source of carbon through remobilization of a large pool of high-molecular weight WSC. Associated quantification of cytokinin levels and expression profiling of putative cytokinin response regulator genes by quantitative reverse transcription polymerase chain reaction support a role for cytokinin in the mediation of the response to N addition in perennial ryegrass. The presence of high levels of cis-zeatin-type cytokinins is discussed in the context of hormonal homeostasis under the stress of steady-state N deficiency.
[Mh] Termos MeSH primário: Carbono/metabolismo
Citocininas/metabolismo
Lolium/fisiologia
Nitratos/farmacologia
Reguladores de Crescimento de Planta/metabolismo
Transdução de Sinais
[Mh] Termos MeSH secundário: Transporte Biológico
Fertilizantes
Cromatografia Gasosa-Espectrometria de Massas
Hidroponia
Lolium/efeitos dos fármacos
Metabolômica
Nitratos/metabolismo
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/fisiologia
Brotos de Planta/efeitos dos fármacos
Brotos de Planta/fisiologia
Solo/química
Zeatina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cytokinins); 0 (Fertilizers); 0 (Nitrates); 0 (Plant Growth Regulators); 0 (Soil); 7440-44-0 (Carbon); 7I6OOJ9GR6 (Zeatin)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151215
[St] Status:MEDLINE
[do] DOI:10.1111/ppl.12412


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[PMID]:26644353
[Au] Autor:Sliwiak J; Dolot R; Michalska K; Szpotkowski K; Bujacz G; Sikorski M; Jaskolski M
[Ad] Endereço:Center for Biocrystallographic Research, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
[Ti] Título:Crystallographic and CD probing of ligand-induced conformational changes in a plant PR-10 protein.
[So] Source:J Struct Biol;193(1):55-66, 2016 Jan.
[Is] ISSN:1095-8657
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plant pathogenesis-related class 10 (PR-10) proteins are a family of abundant proteins initially identified as elements of the plant defense system. The key structural feature suggesting PR-10 functionality is a huge hydrophobic cavity created in the protein interior by a scaffold composed of an extended ß-sheet wrapped around a long and flexible C-terminal α-helix. Several crystallographic and NMR studies have shown that the cavity can accommodate a variety of small molecule ligands, including phytohormones. The article describes ∼1.3 Å resolution crystal structures of a Lupinus luteus PR-10 isoform LlPR-10.1A, in its free form and in complex with trans-zeatin, a naturally occurring plant hormone belonging to the cytokinin group. Moreover we present the structure of the same protein where the saturation with zeatin is not complete. This set of three crystal structures allows us to track the structural adaptation of the protein upon trans-zeatin docking, as well as the sequence of the ligand-binding events, step-by-step. In addition, titration of LlPR-10.1A with trans-zeatin monitored in solution by CD spectra, confirmed the pattern of structural adaptations deduced from the crystallographic studies. The ligand-biding mode shows no similarity to other zeatin complexes of PR-10 proteins. The present work, which describes the first atomic models of the same PR-10 protein with and without a physiological ligand, reveals that the conformation of LlPR-10.1A undergoes a significant structural rearrangement upon trans-zeatin binding.
[Mh] Termos MeSH primário: Reguladores de Crescimento de Planta/metabolismo
Proteínas de Plantas/metabolismo
[Mh] Termos MeSH secundário: Cristalografia por Raios X
Ligantes
Modelos Moleculares
Conformação Proteica
Isoformas de Proteínas/metabolismo
Zeatina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ligands); 0 (Plant Growth Regulators); 0 (Plant Proteins); 0 (Protein Isoforms); 0 (pathogenesis-related proteins, plant); 7I6OOJ9GR6 (Zeatin)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151209
[St] Status:MEDLINE


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[PMID]:26507364
[Au] Autor:Shang XL; Xie RR; Tian H; Wang QL; Guo FQ
[Ad] Endereço:The National Key Laboratory of Plant Molecular Genetics and National Center of Plant Gene Research (Shanghai), Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China.
[Ti] Título:Putative zeatin O-glucosyltransferase OscZOG1 regulates root and shoot development and formation of agronomic traits in rice.
[So] Source:J Integr Plant Biol;58(7):627-41, 2016 Jul.
[Is] ISSN:1744-7909
[Cp] País de publicação:China (Republic : 1949- )
[La] Idioma:eng
[Ab] Resumo:As a ubiquitous reaction, glucosylation controls the bioactivity of cytokinins in plant growth and development. Here we show that genetic manipulation of zeatin-O-glucosylation regulates the formation of important agronomic traits in rice by manipulating the expression of OscZOG1 gene, encoding a putative zeatin O-glucosyltransferase. We found that OscZOG1 was preferentially expressed in shoot and root meristematic tissues and nascent organs. The growth of lateral roots was stimulated in the overexpression lines, but inhibited in RNA interference lines. In shoots, knockdown of OscZOG1 expression by RNA interference significantly improved tillering, panicle branching, grain number per panicle and seed size, which are important agronomic traits for grain yield. In contrast, constitutive expression of OscZOG1 leads to negative effects on the formation of the grain-yielding traits with a marked increase in the accumulation levels of cis-zeatin O-glucoside (cZOG) in the transgenic rice plants. In this study, our findings demonstrate the feasibility of improving the critical yield-determinant agronomic traits, including tiller number, panicle branches, total grain number per panicle and grain weight by downregulating the expression level of OscZOG1. Our results suggest that modulating the levels of cytokinin glucosylation can function as a fine-tuning switch in regulating the formation of agronomic traits in rice.
[Mh] Termos MeSH primário: Glucosiltransferases/metabolismo
Oryza/enzimologia
Oryza/crescimento & desenvolvimento
Raízes de Plantas/crescimento & desenvolvimento
Brotos de Planta/crescimento & desenvolvimento
Característica Quantitativa Herdável
Zeatina/metabolismo
[Mh] Termos MeSH secundário: Regulação da Expressão Gênica de Plantas
Inflorescência/enzimologia
Meristema/enzimologia
Meristema/genética
Oryza/anatomia & histologia
Oryza/genética
Folhas de Planta/enzimologia
Raízes de Plantas/genética
Brotos de Planta/genética
Plantas Geneticamente Modificadas
Interferência de RNA
Plântulas/genética
Plântulas/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
7I6OOJ9GR6 (Zeatin); EC 2.4.1.- (Glucosyltransferases)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170411
[Lr] Data última revisão:
170411
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151029
[St] Status:MEDLINE
[do] DOI:10.1111/jipb.12444



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