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[PMID]:29261720
[Au] Autor:Citro V; Cimmaruta C; Liguori L; Viscido G; Cubellis MV; Andreotti G
[Ad] Endereço:Dipartimento di Biologia, Università Federico II, Napoli, Italy.
[Ti] Título:A mutant of phosphomannomutase1 retains full enzymatic activity, but is not activated by IMP: Possible implications for the disease PMM2-CDG.
[So] Source:PLoS One;12(12):e0189629, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The most frequent disorder of glycosylation, PMM2-CDG, is caused by a deficiency of phosphomannomutase activity. In humans two paralogous enzymes exist, both of them require mannose 1,6-bis-phosphate or glucose 1,6-bis-phosphate as activators, but only phospho-mannomutase1 hydrolyzes bis-phosphate hexoses. Mutations in the gene encoding phosphomannomutase2 are responsible for PMM2-CDG. Although not directly causative of the disease, the role of the paralogous enzyme in the disease should be clarified. Phosphomannomutase1 could have a beneficial effect, contributing to mannose 6-phosphate isomerization, or a detrimental effect, hydrolyzing the bis-phosphate hexose activator. A pivotal role in regulating mannose-1phosphate production and ultimately protein glycosylation might be played by inosine monophosphate that enhances the phosphatase activity of phosphomannomutase1. In this paper we analyzed human phosphomannomutases by conventional enzymatic assays as well as by novel techniques such as 31P-NMR and thermal shift assay. We characterized a triple mutant of phospomannomutase1 that retains mutase and phosphatase activity, but is unable to bind inosine monophosphate.
[Mh] Termos MeSH primário: Defeitos Congênitos da Glicosilação/enzimologia
Defeitos Congênitos da Glicosilação/genética
Inosina Monofosfato/farmacologia
Mutação/genética
Fosfotransferases (Fosfomutases)/deficiência
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Difosfonatos/farmacologia
Ativação Enzimática/efeitos dos fármacos
Ensaios Enzimáticos
Estabilidade Enzimática/efeitos dos fármacos
Seres Humanos
Ligantes
Espectroscopia de Ressonância Magnética
Simulação de Acoplamento Molecular
Fosfotransferases (Fosfomutases)/química
Fosfotransferases (Fosfomutases)/genética
Alinhamento de Sequência
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Diphosphonates); 0 (Ligands); 131-99-7 (Inosine Monophosphate); EC 5.4.2.- (Phosphotransferases (Phosphomutases)); EC 5.4.2.8 (phosphomannomutase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189629


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[PMID]:28340557
[Au] Autor:Yamamoto N; Kawahara R; Akeda Y; Shanmugakani RK; Yoshida H; Hagiya H; Hara N; Nishi I; Yukawa S; Asada R; Sasaki Y; Maeda K; Sakamoto N; Hamada S; Tomono K
[Ad] Endereço:Department of Infection Control and Prevention, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan.
[Ti] Título:Development of selective medium for IMP-type carbapenemase-producing Enterobacteriaceae in stool specimens.
[So] Source:BMC Infect Dis;17(1):229, 2017 Mar 24.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Identification of carbapenemase-producing Enterobacteriaceae (CPE) in faecal specimens is challenging. This fact is particularly critical because low-level carbapenem-resistant organisms such as IMP-producing CPE are most prevalent in Japan. We developed a modified selective medium more suitable for IMP-type CPE. METHODS: Fifteen reference CPE strains producing different types of ß-lactamases were used to evaluate the commercially available CHROMagar KPC and chromID CARBA as well as the newly prepared MC-ECC medium (CHROMagar ECC supplemented with meropenem, cloxacillin, and ZnSO ) and M-ECC medium (CHROMagar ECC supplemented with meropenem and ZnSO ). A total of 1035 clinical samples were then examined to detect CPE using chromID CARBA and M-ECC medium. RESULTS: All tested strains producing NDM-, KPC-, and OXA-48-carbapenemases were successfully cultured in the media employed. Although most of the IMP-positive strains did not grow in CHROMagar KPC, chromID CARBA, or MC-ECC, all tested strains grew on M-ECC. When faecal samples were applied to the media, M-ECC medium allowed the best growth of IMP-type CPE with a significantly higher sensitivity (99.3%) than that of chromID CARBA (13.9%). CONCLUSIONS: M-ECC medium was determined as the most favourable selective medium for the detection of IMP-type CPE as well as other types of CPE.
[Mh] Termos MeSH primário: Proteínas de Bactérias
Técnicas de Tipagem Bacteriana/métodos
Meios de Cultura
Infecções por Enterobacteriaceae
Enterobacteriaceae
Inosina Monofosfato/metabolismo
beta-Lactamases
[Mh] Termos MeSH secundário: Enterobacteriaceae/enzimologia
Enterobacteriaceae/isolamento & purificação
Enterobacteriaceae/metabolismo
Infecções por Enterobacteriaceae/diagnóstico
Infecções por Enterobacteriaceae/microbiologia
Fezes/microbiologia
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Culture Media); 131-99-7 (Inosine Monophosphate); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (carbapenemase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170621
[Lr] Data última revisão:
170621
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170326
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2312-1


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[PMID]:28334294
[Au] Autor:Blonde GD; Spector AC
[Ad] Endereço:Department of Psychology and Program in Neuroscience, Florida State University, Tallahassee, FL, USA.
[Ti] Título:An Examination of the Role of L-Glutamate and Inosine 5'-Monophosphate in Hedonic Taste-Guided Behavior by Mice Lacking the T1R1 + T1R3 Receptor.
[So] Source:Chem Senses;42(5):393-404, 2017 Jun 01.
[Is] ISSN:1464-3553
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The heterodimeric T1R1 + T1R3 receptor is considered critical for normal signaling of L-glutamate and 5'-ribonucleotides in the oral cavity. However, some taste-guided responsiveness remains in mice lacking one subunit of the receptor, suggesting that other receptors are sufficient to support some behaviors. Here, mice lacking both receptor subunits (KO) and wild-type (WT, both n = 13) mice were tested in a battery of behavioral tests. Mice were trained and tested in gustometers with a concentration series of Maltrin-580, a maltodextrin, in a brief-access test (10-s trials) as a positive control. Similar tests followed with monosodium glutamate (MSG) with and without the ribonucleotide inosine 5'-monophosphate (IMP), but always in the presence of the epithelial sodium channel blocker amiloride (A). Brief-access tests were repeated following short-term (30-min) and long-term (48-h) exposures to MSG + A + IMP and were also conducted with sodium gluconate replacing MSG. Finally, progressive ratio tests were conducted with Maltrin-580 or MSG + A + IMP, to assess appetitive behavior while minimizing satiation. Overall, MSG generated little concentration-dependent responding in either food-restricted WT or KO mice, even in combination with IMP. However, KO mice licked less to the amino acid stimuli, a measure of consummatory behavior in the brief-access tests. In contrast, both groups initiated a similar number of trials and had a similar breakpoint in the progressive ratio task, both measures of appetitive (approach) behavior. Collectively, these results suggest that while the T1R1 + T1R3 receptor is necessary for consummatory responding to MSG (+IMP), other receptors are sufficient to maintain appetitive responding to this "umami" stimulus complex in food-restricted mice.
[Mh] Termos MeSH primário: Ácido Glutâmico/farmacologia
Inosina Monofosfato/análogos & derivados
Filosofia
Receptores Acoplados a Proteínas-G/deficiência
Paladar/efeitos dos fármacos
Paladar/fisiologia
[Mh] Termos MeSH secundário: Animais
Feminino
Ácido Glutâmico/administração & dosagem
Inosina Monofosfato/administração & dosagem
Inosina Monofosfato/farmacologia
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, G-Protein-Coupled); 0 (taste receptors, type 1); 131-99-7 (Inosine Monophosphate); 29168-29-4 (inosine 5'-phosphosulfate); 3KX376GY7L (Glutamic Acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.1093/chemse/bjx015


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[PMID]:28202999
[Au] Autor:Xu XX; Cao Y; Fu KY; Xie QF
[Ad] Endereço:Department of Prosthodontics & Center for Oral and Jaw Functional Diagnosis, Treatment and Research, Peking University School and Hospital of Stomatology & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology,
[Ti] Título:[Changes of productions of energy metabolism in masseter of rats induced by occlusal interference].
[So] Source:Beijing Da Xue Xue Bao Yi Xue Ban;49(1):25-30, 2017 02 18.
[Is] ISSN:1671-167X
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To investigate the effect of occlusal interference on the energy metabolism of masticatory muscle by studying the changes of adenosine triphosphate (ATP), adenosine diphosphate (ADP), inosine monophosphate (IMP), phosphocreatine, creatine, lactate and pH level in masseter muscles of rats after occlusal interference. METHODS: Fifty male Sprague-Dawley rats were randomly assigned into experimental group (n=40) and control group (n=10). In experimental group, 0.4 mm thick metal crown was cemented to the upper right first molar of the rat, and maintained for 3, 7, 10, 14 d separately (n=10 for each time point). No occlusal interference was applied for control group. Bilateral masseter muscles of all the rats were acquired under general anesthesia. The samples of 5 rats in each group were fully homogenized with 0.4 mol/L perchlorate (10 mL/g). The homogenates were centrifuged, filtered and analyzed for ATP, ADP, IMP, phosphocreatine, creatine and lactate content by high performance liquid chromatography. The other samples in each group were mixed with homogenates containing 5 mmol/L sodium iodoacetate (10 mL/g), then homogenized and measured for pH value by pH meter in thermostatic water bathunder 37 degrees centigrade. RESULTS: Compared with control group, ATP content in bilateral masseter of the rats increased 3 d after occlusal interference [right side:(5.36±0.13) µmol/g,left side:(5.77±0.25) µmol/g] (P<0.05), and back to normal on 7, 10 and 14 d. There was an increase in IMP [right side:(0.21±0.03) µmol/g,left side:(0.19±0.03) µmol/g]and creatine content [right side:(24.76±2.94) µmol/g,left side:(27.75±2.23) µmol/g]in bilateral masseter of the rats 7 d after occlusal interference (P<0.05) and no difference was detected on 3, 10, and 14. Phosphocreatine content in bilateral masseter started to decline 7 d after occlusal interference and maintained the low level on 10 and 14 d [right side:(10.70±0.71) µmol/g, (11.57±0.52) µmol/g, (10.74±1.39) µmol/g, left side:(10.05±0.57) µmol/g, (10.75±1.12)µmol/g, (10.61±1.15) µmol/g](P<0.05). No change of ADP, lactate or pH level in bilateral muscles of the rats after occlusal interference was observed (P>0.05). CONCLUSION: Occlusal interference influences the content of energy metabolites in masticatory muscle of rats, which may be related to the pathological process of masticatory muscles induced by occlusal interference, such as muscle pain, dysfunction and altered fiber architecture.
[Mh] Termos MeSH primário: Metabolismo Energético/fisiologia
Má Oclusão/fisiopatologia
Músculo Masseter/fisiopatologia
[Mh] Termos MeSH secundário: Difosfato de Adenosina/metabolismo
Trifosfato de Adenosina/metabolismo
Animais
Creatina/metabolismo
Metabolismo Energético/genética
Concentração de Íons de Hidrogênio
Inosina Monofosfato/metabolismo
Ácido Láctico/metabolismo
Masculino
Dente Molar/patologia
Fosfocreatina/metabolismo
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
020IUV4N33 (Phosphocreatine); 131-99-7 (Inosine Monophosphate); 33X04XA5AT (Lactic Acid); 61D2G4IYVH (Adenosine Diphosphate); 8L70Q75FXE (Adenosine Triphosphate); MU72812GK0 (Creatine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170217
[St] Status:MEDLINE


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[PMID]:28167555
[Au] Autor:Matsumura Y; Peirano G; Motyl MR; Adams MD; Chen L; Kreiswirth B; DeVinney R; Pitout JD
[Ad] Endereço:Departments of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada.
[Ti] Título:Global Molecular Epidemiology of IMP-Producing Enterobacteriaceae.
[So] Source:Antimicrob Agents Chemother;61(4), 2017 Apr.
[Is] ISSN:1098-6596
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:International data on the molecular epidemiology of with IMP carbapenemases are lacking. We performed short-read (Illumina) whole-genome sequencing on a global collection of 38 IMP-producing clinical (2008 to 2014). IMP-producing (7 varieties within 11 class 1 integrons) were mainly present in the South Pacific and Asia. Specific -containing integrons (In809 with , In722 with , and In687 with ) were circulating among different bacteria in countries such as Australia, Japan, and Thailand. In1312 with was present in from Japan and from Brazil. ( = 22) was the most common species; clonal complex 14 (CC14) from Philippines and Japan was the most common clone and contained In1310 with and In1321 with The complex ( = 9) consisted of and cluster III. CC78 (from Taiwan) containing In73 with was the most common clone among the complex. This study highlights the importance of surveillance programs using the latest molecular techniques for providing insight into the characteristics and global distribution of with genes.
[Mh] Termos MeSH primário: Enterobacteriaceae/enzimologia
Enterobacteriaceae/genética
Inosina Monofosfato/metabolismo
beta-Lactamases/metabolismo
[Mh] Termos MeSH secundário: Brasil
Citrobacter freundii/enzimologia
Citrobacter freundii/genética
Klebsiella pneumoniae/enzimologia
Klebsiella pneumoniae/genética
Testes de Sensibilidade Microbiana
Epidemiologia Molecular
beta-Lactamases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
131-99-7 (Inosine Monophosphate); EC 3.5.2.6 (beta-Lactamases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170208
[St] Status:MEDLINE


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[PMID]:27596420
[Au] Autor:Li S; Chen L; Hu Y; Fang G; Zhao M; Guo Y; Pang Z
[Ad] Endereço:College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China.
[Ti] Título:Enzymatic production of 5'-inosinic acid by AMP deaminase from a newly isolated Aspergillus oryzae.
[So] Source:Food Chem;216:275-81, 2017 Feb 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:5'-adenylic acid deaminase (AMP deaminase), an important enzyme for the food industry, can catalyze the irreversible hydrolysis of adenosine monophosphate (AMP) to inosine monophosphate (IMP) and ammonia. In this study, a new strain was screened that efficiently produces 3191.6U/g of AMP deaminase at 32°C. After purification, the optimal temperature and pH of the AMP deaminase were found to be 40°C and 6.0, respectively, but it was partially inhibited by Fe(3+), Cu(2+), Al(3+), and Zn(2+). With amplification of the AMP deaminase production system, 6mL of crude enzyme could produce 2.00mg/g of IMP from 2.04mg/g of dried yeast with an 84.8% molar yield after 40min. These results provide a new insight into AMP deaminase production and offer a potential platform for producing 5'-IMP.
[Mh] Termos MeSH primário: AMP Desaminase/análise
AMP Desaminase/biossíntese
Aspergillus oryzae/isolamento & purificação
Inosina Monofosfato/análise
Inosina Monofosfato/biossíntese
[Mh] Termos MeSH secundário: Ativação Enzimática/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
131-99-7 (Inosine Monophosphate); EC 3.5.4.6 (AMP Deaminase)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170104
[Lr] Data última revisão:
170104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160907
[St] Status:MEDLINE


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[PMID]:27461065
[Au] Autor:Suzuki K; Shioura H; Yokota S; Katoh K; Roh SG; Iida F; Komatsu T; Syoji N; Sakuma H; Yamada S
[Ad] Endereço:Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
[Ti] Título:Search for an index for the taste of Japanese Black cattle beef by panel testing and chemical composition analysis.
[So] Source:Anim Sci J;88(3):421-432, 2017 Mar.
[Is] ISSN:1740-0929
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:To search for an index for chemical composition related to superior taste in Japanese Black beef, we conducted panel tests and analyzed the chemical composition of seven beef brands. Thirty-five sirloin beefs from five heifers were used in this study, sold under seven beef brands graded as more than A4 on the Japanese Meat Grade scale. The chemical composition analyses assessed both raw and roasted meat, the latter of which was roasted under the same conditions as those used for the panel test. Results of the panel test and chemical composition analyses revealed that fatty acid composition, sugar content, adenosine triphosphage (ATP)-related compounds, amino acid composition and odor composition in the sirloin meat differed among beef brands. Furthermore, the correlations of chemical compositions between roasted and raw meat were significantly high. Sugar content and ATP-related compounds in roasted meat were significantly correlated with the item 'overall evaluation' of the panel test. ATP-related compounds, such as inosinic acid, carnosine and taurine, in roasted and raw meat were correlated significantly with the item 'umami intensity' of the panel test. These results suggest that the composition of these components is important for an index related to the overall evaluation of beef.
[Mh] Termos MeSH primário: Aminoácidos/análise
Carboidratos/análise
Bovinos
Ácidos Graxos/análise
Qualidade dos Alimentos
Carne
Paladar/fisiologia
[Mh] Termos MeSH secundário: Animais
Carnosina/análise
Culinária/métodos
Feminino
Seres Humanos
Inosina Monofosfato/análise
Carne/análise
Característica Quantitativa Herdável
Taurina/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Carbohydrates); 0 (Fatty Acids); 131-99-7 (Inosine Monophosphate); 1EQV5MLY3D (Taurine); 8HO6PVN24W (Carnosine)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170420
[Lr] Data última revisão:
170420
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160728
[St] Status:MEDLINE
[do] DOI:10.1111/asj.12663


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[PMID]:27613871
[Au] Autor:Rosenberg MM; Redfield AG; Roberts MF; Hedstrom L
[Ad] Endereço:From the Departments of Biology.
[Ti] Título:Substrate and Cofactor Dynamics on Guanosine Monophosphate Reductase Probed by High Resolution Field Cycling 31P NMR Relaxometry.
[So] Source:J Biol Chem;291(44):22988-22998, 2016 10 28.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Guanosine-5'-monophosphate reductase (GMPR) catalyzes the reduction of GMP to IMP and ammonia with concomitant oxidation of NADPH. Here we investigated the structure and dynamics of enzyme-bound substrates and cofactors by measuring P relaxation rates over a large magnetic field range using high resolution field cycling NMR relaxometry. Surprisingly, these experiments reveal differences in the low field relaxation profiles for the monophosphate of GMP compared with IMP in their respective NADP complexes. These complexes undergo partial reactions that mimic different steps in the overall catalytic cycle. The relaxation profiles indicate that the substrate monophosphates have distinct interactions in E·IMP·NADP and E·GMP·NADP complexes. These findings were not anticipated by x-ray crystal structures, which show identical interactions for the monophosphates of GMP and IMP in several inert complexes. In addition, the motion of the cofactor is enhanced in the E·GMP·NADP complex. Last, the motions of the substrate and cofactor are coordinately regulated; the cofactor has faster local motions than GMP in the deamination complex but is more constrained than IMP in that complex, leading to hydride transfer. These results show that field cycling can be used to investigate the dynamics of protein-bound ligands and provide new insights into how portions of the substrate remote from the site of chemical transformation promote catalysis.
[Mh] Termos MeSH primário: Coenzimas/química
Proteínas de Escherichia coli/química
Escherichia coli/enzimologia
GMP Redutase/química
[Mh] Termos MeSH secundário: Biocatálise
Coenzimas/metabolismo
Cristalografia por Raios X
Escherichia coli/química
Escherichia coli/genética
Proteínas de Escherichia coli/genética
Proteínas de Escherichia coli/metabolismo
GMP Redutase/genética
GMP Redutase/metabolismo
Nucleotídeos de Guanina/química
Nucleotídeos de Guanina/metabolismo
Inosina Monofosfato/química
Inosina Monofosfato/metabolismo
Cinética
Espectroscopia de Ressonância Magnética
NADP/química
NADP/metabolismo
Ligação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Coenzymes); 0 (Escherichia coli Proteins); 0 (Guanine Nucleotides); 131-99-7 (Inosine Monophosphate); 53-59-8 (NADP); EC 1.7.1.7 (GMP Reductase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160911
[St] Status:MEDLINE


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[PMID]:27601249
[Au] Autor:Yamashita K; Uchiyama Y; Ofuji A; Mimura H; Okumiya S; Takaki A; Sone T; Ito S
[Ad] Endereço:Graduate School of Health Sciences, Kumamoto University, 4-24-1 Kuhonji, Chuo-ku, Kumamoto 862-0796, Japan; Fujifilm RI Pharma Co. Ltd., Kyobashi 14-1 2-chome, Chuo-ku, Tokyo 104-0031, Japan.
[Ti] Título:Fully automatic input function determination program for simple noninvasive (123)I-IMP microsphere cerebral blood flow quantification method.
[So] Source:Phys Med;32(9):1180-5, 2016 Sep.
[Is] ISSN:1724-191X
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:We recently developed a simple noninvasive (123)I-IMP microsphere (SIMS) method using chest dynamic planar images and brain single photon emission computed tomography. The SIMS method is an automatic analysis method, except for the process of setting the region of interest (ROI) of the input function. If a fully automatic ROI setting algorithm can be developed to determine the input function for the SIMS method, repeatability and reproducibility of the analysis of regional cerebral blood flow (rCBF) of the SIMS method can be guaranteed. The purpose of this study is to develop a fully automatic input function determination program for the SIMS method and to confirm the clinical usefulness of this program. The automatic input function determination program consists of two ROI setting programs for the PA and lung regions, and it is developed using the image phase analysis of a chest RI angiogram. To confirm the clinical usefulness of this program, the rCBF in 34 patients measured using the automatic method were compared with the values obtained through the manual setting method. Input functions by the automatic and manual methods were approximately equal. A good correlation was observed between the rCBF values obtained by the automatic method and those obtained by the manual setting method (r=0.96, p<0.01). Further, the total time taken for the automatic SIMS analysis is 1-2min as compared to 20-30min for the current analysis, and therefore, this technique contributes to the improvement of the throughput of nuclear medical examinations.
[Mh] Termos MeSH primário: Circulação Cerebrovascular
Processamento de Imagem Assistida por Computador/métodos
Inosina Monofosfato/química
Radioisótopos do Iodo/química
Tomografia Computadorizada de Emissão de Fóton Único
[Mh] Termos MeSH secundário: Idoso
Angiografia
Área Sob a Curva
Automação
Feminino
Seres Humanos
Pulmão/diagnóstico por imagem
Masculino
Microesferas
Meia-Idade
Reconhecimento Automatizado de Padrão
Artéria Pulmonar/diagnóstico por imagem
Compostos Radiofarmacêuticos
Análise de Regressão
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Iodine Radioisotopes); 0 (Radiopharmaceuticals); 131-99-7 (Inosine Monophosphate)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170330
[Lr] Data última revisão:
170330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160908
[St] Status:MEDLINE


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[PMID]:27539573
[Au] Autor:Li S; Qian Y; Liang Y; Chen X; Zhao M; Guo Y; Pang Z
[Ad] Endereço:College of Light Industry and Food Engineering, Guangxi University, Nanning, 530004, China.
[Ti] Título:Overproduction, Purification and Characterization of Adenylate Deaminase from Aspergillus oryzae.
[So] Source:Appl Biochem Biotechnol;180(8):1635-1643, 2016 Dec.
[Is] ISSN:1559-0291
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Adenylate deaminase (AMPD, EC 3.5.4.6) is an aminohydrolase that widely used in the food and medicine industries. In this study, the gene encoding Aspergillus oryzae AMPD was cloned and expressed in Escherichia coli. Induction with 0.75 mM isopropyl ß-D-l-thiogalactopyranoside resulted in an enzyme activity of 1773.9 U/mL. Recombinant AMPD was purified to electrophoretic homogeneity using nickel affinity chromatography, and its molecular weight was calculated as 78.6 kDa. Purified AMPD exhibited maximal activity at 35 °C, pH 6.0 and 30 mM K , with apparent K and V values of 2.7 × 10 M and 77.5 µmol/mg/min under these conditions. HPLC revealed that recombinant AMPD could effectively catalyse the synthesis of inosine-5'-monophosphate (IMP) with minimal by-products, indicating high specificity and suggesting that it could prove useful for IMP production.
[Mh] Termos MeSH primário: AMP Desaminase/isolamento & purificação
AMP Desaminase/metabolismo
Aspergillus oryzae/enzimologia
[Mh] Termos MeSH secundário: Monofosfato de Adenosina/química
Monofosfato de Adenosina/metabolismo
Aspergillus oryzae/efeitos dos fármacos
Biocatálise/efeitos dos fármacos
Clonagem Molecular
Eletroforese em Gel de Poliacrilamida
Estabilidade Enzimática/efeitos dos fármacos
Concentração de Íons de Hidrogênio
Inosina Monofosfato/química
Inosina Monofosfato/metabolismo
Íons
Cinética
Metais/farmacologia
Peso Molecular
Proteínas Recombinantes/metabolismo
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ions); 0 (Metals); 0 (Recombinant Proteins); 131-99-7 (Inosine Monophosphate); 415SHH325A (Adenosine Monophosphate); EC 3.5.4.6 (AMP Deaminase)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170113
[Lr] Data última revisão:
170113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160820
[St] Status:MEDLINE



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