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  1 / 105 MEDLINE  
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[PMID]:28090749
[Au] Autor:Bachas S; Kohrs B; Wade H
[Ad] Endereço:Laboratory of RNA Biophysics and Cellular Physiology, Biochemistry and Biophysics Center, National Institutes of Health, 50 South Drive, MSC, Bethesda, MD, 20892-8012, USA.
[Ti] Título:Unconventional Coupling between Ligand Recognition and Allosteric Control in the Multidrug Resistance Gene Regulator, BmrR.
[So] Source:ChemMedChem;12(6):426-430, 2017 Mar 17.
[Is] ISSN:1860-7187
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:BmrR is a multidrug resistance (MDR) regulator that responds to diverse ligands. To obtain insight into signal recognition, allosteric control, and cooperativity, we used a quantitative in vitro transcription assay to determine the ligand-dependent activation profiles for a diverse set of cations, zwitterions, and uncharged ligands. As for many other biological switch systems, the data are well described by a modified Hill equation. Parameters extracted from curve fits to the data include L , R and N. We found that L values correlate directly with ΔG values, suggesting that the parameter reflects binding, whereas R and N reflect allosteric control and cooperativity, respectively. Our results suggest unconventional coupling between ligand binding and allosteric control, with weakly interacting ligands exhibiting the highest levels of activation. Such properties are in stark contrast to those often exhibited by biological switch proteins, whereby ligand binding and allostery are tightly coupled, yielding both high selectivity and ultrasensitivity. We propose that weakened coupling, as observed for BmrR, may be important for providing robust activation responses to unrelated ligands. We also propose that other MDR proteins and other polyspecific switch systems will show similar features.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Ligantes
Transativadores/metabolismo
[Mh] Termos MeSH secundário: Regulação Alostérica
Aminoquinolinas/química
Aminoquinolinas/farmacologia
Bacillus subtilis/metabolismo
Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos
Regiões Promotoras Genéticas
Quinaldinas/química
Quinaldinas/farmacologia
Termodinâmica
Transativadores/química
Transativadores/genética
Ativação Transcricional/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (4-amino-2-methylquinoline); 0 (Aminoquinolines); 0 (Bacterial Proteins); 0 (BmrR protein, bacteria); 0 (Ligands); 0 (Quinaldines); 0 (Trans-Activators)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170630
[Lr] Data última revisão:
170630
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170117
[St] Status:MEDLINE
[do] DOI:10.1002/cmdc.201700017


  2 / 105 MEDLINE  
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[PMID]:27748837
[Au] Autor:Liu CY; Hsieh CH; Kim SH; Wang JP; Ni YL; Su CL; Yao CF; Fang K
[Ad] Endereço:Department of Life Science, National Taiwan Normal University, Taipei 116, Taiwan, R.O.C.
[Ti] Título:An indolylquinoline derivative activates DNA damage response and apoptosis in human hepatocellular carcinoma cells.
[So] Source:Int J Oncol;49(6):2431-2441, 2016 Dec.
[Is] ISSN:1791-2423
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Human liver cancer is one of the most frequently diagnosed cancers worldwide. The development of resistance to therapy limits the application against the disease. To improve treatment, new effective anticancer agents are constantly pursued. Previously, we reported that an indolylquinoline, 3-((7-ethyl-1H-indol-3-yl)-methyl)-2-methylquinoline (EMMQ), is effective in suppressing the growth of human lung cancer by impairing mitochondria functions. The present study revealed that EMMQ inhibited cell growth and induced apoptosis in liver cancer cells, but not in normal cells. This study demonstrated that EMMQ induced DNA damage by activating p53 and γ-H2AX and cell arrest by suppressing cyclin D1 and CDK2. Damaged DNA injured mitochondrial functions by lowering the membrane potential and producing reactive oxygen species. The subsequent mitochondrial cytochrome c release attenuated pro-survival signals and increased apoptotic characteristics. Introduction of p53 shRNA abrogated drug effects by reducing DNA damage while maintaining mitochondria integrity. In brief, the study demonstrates that the effectiveness of EMMQ accentuated apoptosis of hepatocarcinoma cells by activating p53. Based on these collective findings, the study offered a new perspective of EMMQ that was shown to be a promising candidate to treat liver cancer.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Carcinoma Hepatocelular/patologia
Dano ao DNA/efeitos dos fármacos
Indóis/farmacologia
Neoplasias Hepáticas/patologia
Fígado/patologia
Quinaldinas/farmacologia
Quinolinas/farmacologia
Proteína Supressora de Tumor p53/genética
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Ciclina D1/antagonistas & inibidores
Quinase 2 Dependente de Ciclina/antagonistas & inibidores
Citocromos c/secreção
Ativação Enzimática/efeitos dos fármacos
Pontos de Checagem da Fase G1 do Ciclo Celular/efeitos dos fármacos
Células Hep G2
Histonas/metabolismo
Seres Humanos
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/genética
Quinaldinas/química
Interferência de RNA
RNA Interferente Pequeno/genética
Espécies Reativas de Oxigênio/metabolismo
Proteína Supressora de Tumor p53/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3-((7-ethyl-1H-indol-3-yl)-methyl)-2-methylquinoline); 0 (CCND1 protein, human); 0 (H2AFX protein, human); 0 (Histones); 0 (Indoles); 0 (Quinaldines); 0 (Quinolines); 0 (RNA, Small Interfering); 0 (Reactive Oxygen Species); 0 (TP53 protein, human); 0 (Tumor Suppressor Protein p53); 136601-57-5 (Cyclin D1); 9007-43-6 (Cytochromes c); DVG30M0M87 (2-methylquinoline); EC 2.7.11.22 (CDK2 protein, human); EC 2.7.11.22 (Cyclin-Dependent Kinase 2)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170622
[Lr] Data última revisão:
170622
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161018
[St] Status:MEDLINE
[do] DOI:10.3892/ijo.2016.3717


  3 / 105 MEDLINE  
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[PMID]:27347919
[Au] Autor:Le ST; Yasuoka C; Asahara H; Nishiwaki N
[Ad] Endereço:Faculty of Applied Sciences, Ton Duc Thang University, 19 Nguyen Huu Tho Stress, Tan Phong Ward, District 7, Ho Chi Minh City 700000, Vietnam. lethisong@tdt.edu.vn.
[Ti] Título:Dual Behavior of Iodine Species in Condensation of Anilines and Vinyl Ethers Affording 2-Methylquinolines.
[So] Source:Molecules;21(7), 2016 Jun 25.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:A metal-free, mild and efficient method for the synthesis of 2-methylquinolines was successfully developed by condensation of anilines with vinyl ethers in the presence of catalytic amount of iodine. Modification of both pyridine and benzene moieties was easily achieved by changing only the vinyl ether and aniline. In this reaction, the iodine species was revealed to show dual behavior; molecular iodine serves as an oxidant, while its reduced form, hydrogen iodide, activates the vinyl ether. The redox reaction between these iodine species enables the use of a catalytic amount of iodine in this synthetic method.
[Mh] Termos MeSH primário: Compostos de Anilina/química
Iodo/química
Quinaldinas/química
Compostos de Vinila/química
[Mh] Termos MeSH secundário: Catálise
Espectroscopia de Ressonância Magnética
Estrutura Molecular
Oxirredução
Quinaldinas/síntese química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aniline Compounds); 0 (Quinaldines); 0 (Vinyl Compounds); 2H2T044E11 (vinyl ether); 9679TC07X4 (Iodine); DVG30M0M87 (2-methylquinoline)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170418
[Lr] Data última revisão:
170418
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160628
[St] Status:MEDLINE


  4 / 105 MEDLINE  
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[PMID]:26053552
[Au] Autor:Xie H; Liao Y; Chen S; Chen Y; Deng GJ
[Ad] Endereço:Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 411105, China. gjdeng@xtu.edu.cn.
[Ti] Título:Copper-catalyzed efficient direct amidation of 2-methylquinolines with amines.
[So] Source:Org Biomol Chem;13(25):6944-8, 2015 Jul 07.
[Is] ISSN:1477-0539
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel Cu-catalyzed direct amidation of 2-methylquinolines with amines is described. This method afforded an efficient approach for the synthesis of biologically important aromatic amides from readily available coupling partners using molecular oxygen as the oxidant.
[Mh] Termos MeSH primário: Amidas/síntese química
Aminas/química
Cobre/química
Hidrocarbonetos Aromáticos/síntese química
Quinaldinas/química
[Mh] Termos MeSH secundário: Amidas/química
Aminas/síntese química
Catálise
Hidrocarbonetos Aromáticos/química
Oxidantes/química
Oxirredução
Oxigênio/química
Quinaldinas/síntese química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amides); 0 (Amines); 0 (Hydrocarbons, Aromatic); 0 (Oxidants); 0 (Quinaldines); 789U1901C5 (Copper); DVG30M0M87 (2-methylquinoline); S88TT14065 (Oxygen)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:150618
[Lr] Data última revisão:
150618
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150609
[St] Status:MEDLINE
[do] DOI:10.1039/c5ob00915d


  5 / 105 MEDLINE  
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[PMID]:25415851
[Au] Autor:Xiao F; Chen S; Chen Y; Huang H; Deng GJ
[Ad] Endereço:Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 411105, China. 494707822@163.com gjdeng@xtu.edu.cn.
[Ti] Título:Efficient 2-sulfolmethyl quinoline formation from 2-methylquinolines and sodium sulfinates under transition-metal free conditions.
[So] Source:Chem Commun (Camb);51(4):652-4, 2015 Jan 14.
[Is] ISSN:1364-548X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An efficient procedure for 2-sulfolmethyl quinoline preparation from 2-methylquinolines and sodium sulfinates under transition-metal free conditions is described. Halogen functional groups were well tolerated to give the corresponding products in good to high yields.
[Mh] Termos MeSH primário: Quinaldinas/síntese química
Ácidos Sulfínicos/química
Compostos de Enxofre/síntese química
[Mh] Termos MeSH secundário: Halogênios/química
Quinaldinas/química
Sódio/química
Compostos de Enxofre/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Halogens); 0 (Quinaldines); 0 (Sulfinic Acids); 0 (Sulfur Compounds); 9NEZ333N27 (Sodium); DVG30M0M87 (2-methylquinoline)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:141216
[Lr] Data última revisão:
141216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141122
[St] Status:MEDLINE
[do] DOI:10.1039/c4cc07546c


  6 / 105 MEDLINE  
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[PMID]:24704486
[Au] Autor:Yan Q; Li L; Li W; Wang L; Sun WH
[Ad] Endereço:Key Laboratory of Engineering Plastics and Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
[Ti] Título:Synthesis, characterization and fluorescent properties of 5-(aryliminomethyl)quinaldine-8-ol derivatives and their trinuclear zinc complexes.
[So] Source:Spectrochim Acta A Mol Biomol Spectrosc;128:790-7, 2014 Jul 15.
[Is] ISSN:1873-3557
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A series of 5-[1-(arylimino)methyl]quinaldine-8-ol derivatives L1-L5 and their trinuclear zinc(II) complexes (C1-C5) were synthesized. The compounds L1-L5 were fully characterized by the FT-IR spectra, NMR measurement and elemental analysis, meanwhile the zinc complexes C1-C5 were characterized by the FT-IR spectra and elemental analysis as well as the single crystal X-ray diffraction of a representative complex C3, which revealed a trinuclear zinc complex bearing six organic ligands. The fluorescent properties of both organic compounds and the zinc complexes have been carefully investigated by the UV-Vis absorption in various solvents, indicating the significant influences of the solvents and also double exponential decays.
[Mh] Termos MeSH primário: Corantes Fluorescentes/química
Corantes Fluorescentes/síntese química
Quinaldinas/química
Quinaldinas/síntese química
Zinco
[Mh] Termos MeSH secundário: Estrutura Molecular
Espectrofotometria Ultravioleta/métodos
Espectroscopia de Infravermelho com Transformada de Fourier/métodos
Difração de Raios X/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fluorescent Dyes); 0 (Quinaldines); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:140424
[Lr] Data última revisão:
140424
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140408
[St] Status:MEDLINE


  7 / 105 MEDLINE  
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[PMID]:24117081
[Au] Autor:Wang L; Li Y; Duan J
[Ad] Endereço:a State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering , Tongji University , Shanghai , People's Republic of China.
[Ti] Título:Biodegradation of 2-methylquinoline by Klebsiella pneumoniae TJ-A isolated from acclimated activated sludge.
[So] Source:J Environ Sci Health A Tox Hazard Subst Environ Eng;49(1):27-38, 2014.
[Is] ISSN:1532-4117
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bacterial strain Klebsiella pneumoniae TJ-A, which was capable of utilizing 2-methylquinoline as the sole carbon and energy source, was isolated from acclimated activated sludge under aerobic conditions. Effects of temperature and initial pH on the biodegradation of 2-methylquinoline by Klebsiella pneumoniae TJ-A were investigated. The optimal temperature and initial pH were 30°C and 7.5, respectively. The degradation process was well described by the Haldane model. Then 1, 2, 3, 4-tetrahydro-2-methylquinoline, 4-ethyl-benzenamine and N-butyl-benzenamine were metabolites detected during the degradation of 2-methylquinoline. 2-Methylquinoline was initially hydroxylated at C-4 to form 2-methyl-4-hydroxy-quinoline, and then to form 2-methyl-4-quinolinol as a result of tautomerism. Hydrogenation of heterocyclic ring between the position 2 and 3 produced 2, 3-dihydro-2-methyl-4-quinolinol. The carbon-carbon bond between the position 2 and 3 in the heterocyclic ring cleaved and then formed 2-ethyl-N-ethyl-benzenamine. Tautomerism might result in the formation of N-butyl-benzenamine. The 4-ethyl-benzenamine was produced as a result of losing one ethyl group from N-butyl-benzenamine. The bacterial strain Klebsiella pneumoniae TJ-A was the priority species in the aerobic activated sludge responsible for the degradation of 2-methylquinoline.
[Mh] Termos MeSH primário: Klebsiella pneumoniae/metabolismo
Quinaldinas/metabolismo
Esgotos/microbiologia
[Mh] Termos MeSH secundário: Aerobiose
Biodegradação Ambiental
Concentração de Íons de Hidrogênio
Klebsiella pneumoniae/isolamento & purificação
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Quinaldines); 0 (Sewage); DVG30M0M87 (2-methylquinoline)
[Em] Mês de entrada:1405
[Cu] Atualização por classe:160526
[Lr] Data última revisão:
160526
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131015
[St] Status:MEDLINE
[do] DOI:10.1080/10934529.2013.824228


  8 / 105 MEDLINE  
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[PMID]:24218841
[Au] Autor:Wang L; Li Y; Duan J
[Ad] Endereço:State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China.
[Ti] Título:Biodegradation of 2-methylquinoline by Enterobacter aerogenes TJ-D isolated from activated sludge.
[So] Source:J Environ Sci (China);25(7):1310-8, 2013 Jul 01.
[Is] ISSN:1001-0742
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Bacterial strain Enterobacter aerogenes TJ-D capable of utilizing 2-methylquinoline as the sole carbon and energy source was isolated from acclimated activated sludge under denitrifying conditions. The ability to degrade 2-methylquinoline by E. aerogenes TJ-D was investigated under denitrifying conditions. Under optimal conditions of temperature (35 degrees C) and initial pH 7, 2-methylquinoline of 100 mg/L was degraded within 176 hr. The degradation of 2-methylquinoline by E. aerogenes TJ-D could be well described by the Haldane model (R2 > 0.91). During the degradation period of 2-methylquinoline (initial concentration 100 mg/L), nitrate was almost completely consumed (the removal efficiency was 98.5%), while nitrite remained at low concentration (< 0.62 mg/L) during the whole denitrification period. 1,2,3,4-Tetrahydro-2-methylquinoline, 4-ethyl-benzenamine, N-butyl-benzenamine, N-ethyl-benzenamine and 2,6-diethyl-benzenamine were metabolites produced during the degradation. The degradation pathway of 2-methylquinoline by E. aerogenes TJ-D was proposed. 2-Methylquinoline is initially hydroxylated at C-4 to form 2-methyl-4-hydroxy-quinoline, and then forms 2-methyl-4-quinolinol as a result of tautomerism. Hydrogenation of the heterocyclic ring at positions 2 and 3 produces 2,3-dihydro-2-methyl-4-quinolinol. The carbon-carbon bond at position 2 and 3 in the heterocyclic ring may cleave and form 2-ethyl-N-ethyl-benzenamine. Tautomerism may result in the formation of 2,6-diethyl-benzenamine and N-butyl-benzenamine. 4-Ethyl-benzenamine and N-ethyl-benzenamine were produced as a result of losing one ethyl group from the above molecules.
[Mh] Termos MeSH primário: Enterobacter aerogenes/metabolismo
Quinaldinas/metabolismo
Poluentes Químicos da Água/metabolismo
[Mh] Termos MeSH secundário: Sequência de Bases
Biodegradação Ambiental
DNA Bacteriano/genética
Desnitrificação
Enterobacter aerogenes/genética
Enterobacter aerogenes/isolamento & purificação
Concentração de Íons de Hidrogênio
Dados de Sequência Molecular
Filogenia
Análise de Sequência de DNA
Esgotos/microbiologia
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Quinaldines); 0 (Sewage); 0 (Water Pollutants, Chemical); DVG30M0M87 (2-methylquinoline)
[Em] Mês de entrada:1402
[Cu] Atualização por classe:131113
[Lr] Data última revisão:
131113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131114
[St] Status:MEDLINE


  9 / 105 MEDLINE  
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[PMID]:23841224
[Au] Autor:Fagerer SR; Nielsen S; Ibáñez A; Zenobi R
[Ad] Endereço:Department of Chemistry and Applied Biosciences, ETH Zurich, CH-8093 Zurich, Switzerland.
[Ti] Título:Matrix-assisted laser desorption/ionization matrices for negative mode metabolomics.
[So] Source:Eur J Mass Spectrom (Chichester);19(1):39-47, 2013.
[Is] ISSN:1469-0667
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Matrix-assisted laser desorption/ionization (MALDI) has been shown to be highly sensitive for analyzing low-mass compounds such as metabolites if the right matrix is used. 9-aminoacridine (9AA) is the most commonly employed matrix for negative mode MALDI-MS in metabolomics. However, matrix interferences and the strongly varying sensitivity for different metabolites make a search for alternative matrices desirable, in order to identify compounds with a different chemical background and/or favoring a different range of analytes. We tested the performance of a series of potential negative mode MALDI matrices with a mix of 29 metabolites containing amino acids, nucleotide phosphates and Krebs cycle intermediates. While ethacridine lactate was found to provide limits of detection (LODs) in the low femtomole range for nucleotide phosphates, amino acids and Krebs cycle intermediates in the low picomole range, 4-amino-2-methylquinoline showed LODs in the picomole range for most metabolites, but is capable of ionizing a broader range of analytes than both 9AA and ethacridine.
[Mh] Termos MeSH primário: Metabolômica/métodos
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
[Mh] Termos MeSH secundário: Aminacrina/química
Aminoácidos/análise
Aminoácidos/química
Aminoquinolinas/química
Etacridina/química
Limite de Detecção
Nucleotídeos/análise
Nucleotídeos/química
Quinaldinas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (4-amino-2-methylquinoline); 0 (Amino Acids); 0 (Aminoquinolines); 0 (Nucleotides); 0 (Quinaldines); 78OY3Z0P7Z (Aminacrine); WIX85M1A6R (Ethacridine)
[Em] Mês de entrada:1308
[Cu] Atualização por classe:160919
[Lr] Data última revisão:
160919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130712
[St] Status:MEDLINE


  10 / 105 MEDLINE  
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[PMID]:23275246
[Au] Autor:Niewerth H; Parschat K; Rauschenberg M; Ravoo BJ; Fetzner S
[Ad] Endereço:Institute of Molecular Microbiology and Biotechnology, Westfalian Wilhelms-University Münster, Münster, Germany.
[Ti] Título:The PaaX-type repressor MeqR2 of Arthrobacter sp. strain Rue61a, involved in the regulation of quinaldine catabolism, binds to its own promoter and to catabolic promoters and specifically responds to anthraniloyl coenzyme A.
[So] Source:J Bacteriol;195(5):1068-80, 2013 Mar.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The genes coding for quinaldine catabolism in Arthrobacter sp. strain Rue61a are clustered on the linear plasmid pAL1 in two upper pathway operons (meqABC and meqDEF) coding for quinaldine conversion to anthranilate and a lower pathway operon encoding anthranilate degradation via coenzyme A (CoA) thioester intermediates. The meqR2 gene, located immediately downstream of the catabolic genes, codes for a PaaX-type transcriptional repressor. MeqR2, purified as recombinant fusion protein, forms a dimer in solution and shows specific and cooperative binding to promoter DNA in vitro. DNA fragments recognized by MeqR2 contained a highly conserved palindromic motif, 5'-TGACGNNCGTcA-3', which is located at positions -35 to -24 of the two promoters that control the upper pathway operons, at positions +4 to +15 of the promoter of the lower pathway genes and at positions +53 to +64 of the meqR2 promoter. Disruption of the palindrome abolished MeqR2 binding. The dissociation constants (K(D)) of MeqR2-DNA complexes as deduced from electrophoretic mobility shift assays were very similar for the four promoters tested (23 nM to 28 nM). Anthraniloyl-CoA was identified as the specific effector of MeqR2, which impairs MeqR2-DNA complex formation in vitro. A binding stoichiometry of one effector molecule per MeqR2 monomer and a K(D) of 22 nM were determined for the effector-protein complex by isothermal titration calorimetry (ITC). Quantitative reverse transcriptase PCR analyses suggested that MeqR2 is a potent regulator of the meqDEF operon; however, additional regulatory systems have a major impact on transcriptional control of the catabolic operons and of meqR2.
[Mh] Termos MeSH primário: Arthrobacter/genética
Arthrobacter/metabolismo
Proteínas de Bactérias/metabolismo
Coenzima A/metabolismo
Regiões Promotoras Genéticas
Quinaldinas/metabolismo
Proteínas Repressoras/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Coenzima A/genética
DNA Bacteriano/genética
DNA Bacteriano/metabolismo
Proteínas de Ligação a DNA/metabolismo
Ensaio de Desvio de Mobilidade Eletroforética
Regulação Bacteriana da Expressão Gênica
Ligação Proteica
Proteínas Recombinantes de Fusão/metabolismo
Proteínas Repressoras/genética
Transcrição Genética
ortoaminobenzoatos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (DNA-Binding Proteins); 0 (Quinaldines); 0 (Recombinant Fusion Proteins); 0 (Repressor Proteins); 0 (ortho-Aminobenzoates); 0YS975XI6W (anthranilic acid); DVG30M0M87 (2-methylquinoline); SAA04E81UX (Coenzyme A)
[Em] Mês de entrada:1304
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130101
[St] Status:MEDLINE
[do] DOI:10.1128/JB.01547-12



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