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Pesquisa : D03.633.300.046.250.150 [Categoria DeCS]
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  1 / 2008 MEDLINE  
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[PMID]:29277813
[Au] Autor:Kusuzaki K; Takai T; Yoshimura H; Inoue K; Takai S; Baldini N
[Ad] Endereço:Department of Musculoskeletal Oncology, Tenri, Japan 18kusu43@gmail.com.
[Ti] Título:Clinical Trial of Radiotherapy After Intravenous Injection of Acridine Orange for Patients with Cancer.
[So] Source:Anticancer Res;38(1):481-489, 2018 01.
[Is] ISSN:1791-7530
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:AIM: We previously found that low-dose X-ray treatment after systemic administration of acridine orange (AO), which is known to have a low toxicity in animals, inhibited tumor growth in experimental studies using mouse osteosarcoma. In this pilot study, we planned to verify the toxicity of intravenous injection of low-dose AO in humans and investigate the anticancer effect of radiation after systemic AO administration (iAOR) for human cancer. PATIENTS AND METHODS: Eight patients with terminal cancer were treated with iAOR. RESULTS: None of the patients exhibited an adverse effect from AO injection. Three out of the five patients who received a full course of iAOR exhibited clinical or image-based responses, whereas two patients did not. CONCLUSION: The systemic administration of AO was confirmed not to be toxic in humans, and iAOR was suggested to be potentially effective against radioresistant cancer.
[Mh] Termos MeSH primário: Laranja Acridina/toxicidade
Laranja Acridina/uso terapêutico
Mutagênicos/uso terapêutico
Neoplasias/tratamento farmacológico
Neoplasias/radioterapia
[Mh] Termos MeSH secundário: Seres Humanos
Mutagênicos/toxicidade
Projetos Piloto
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mutagens); F30N4O6XVV (Acridine Orange)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171227
[St] Status:MEDLINE


  2 / 2008 MEDLINE  
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[PMID]:28750078
[Au] Autor:Kwizera R; Akampurira A; Williams D; Boulware DR; Meya DB; ASTRO-CM Study Team
[Ad] Endereço:Infectious Diseases Institute, College of Health Sciences, Makerere University, Kampala, Uganda.
[Ti] Título:Acridine orange fluorescent microscopy is more sensitive than India ink light microscopy in the rapid detection of cryptococcosis among CrAg positive HIV patients.
[So] Source:PLoS One;12(7):e0182108, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: India ink microscopy on cerebrospinal fluid is still utilized in resource limited settings for the diagnosis of cryptococcal meningitis despite its poor sensitivity. We hypothesized that staining fungal nucleic acids with fluorescent dyes instead of the capsule with India ink might improve sensitivity for the diagnosis of cryptococcal meningitis. METHODS: We enrolled 96 HIV-infected participants with cryptococcal meningitis who provided 194 CSF specimens at serial time points in Kampala, Uganda. Cryptococcosis was diagnosed by cerebrospinal fluid (CSF) cryptococcal antigen (CrAg) test and only positive samples were included. We stained CSF with India ink and acridine orange. We cultured the same samples on standard fungal media. We compared acridine orange to CrAg, India ink and CSF culture. RESULTS: Acridine orange was more sensitive (96%) than India ink (79%) with reference to CSF CrAg. Acridine orange and India ink had a statistically significant difference (P<0.001) with a 25% correlation for detection of Cryptococcus yeasts. India ink had more negative results (22%) than acridine orange (4%). The sensitivity for India ink increased (86%) while that of acridine orange did not change (97%) when compared to CSF culture. However, both India ink and acridine orange had poor predictive values with reference to culture. CONCLUSION: Acridine orange is a better alternative to India ink in the rapid detection of cryptococcosis among CrAg positive HIV patients.
[Mh] Termos MeSH primário: Laranja Acridina/química
Antígenos de Fungos/imunologia
Carbono/química
Criptococose/diagnóstico
Cryptococcus/imunologia
Infecções por HIV/complicações
Infecções por HIV/microbiologia
Microscopia de Fluorescência/métodos
[Mh] Termos MeSH secundário: Adulto
Criptococose/microbiologia
Cryptococcus/citologia
Feminino
Seres Humanos
Masculino
Meningite Criptocócica/diagnóstico
Meningite Criptocócica/microbiologia
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Fungal); 0 (chinese ink); 7440-44-0 (Carbon); F30N4O6XVV (Acridine Orange)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182108


  3 / 2008 MEDLINE  
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[PMID]:28750048
[Au] Autor:Patiño-Ruiz M; Ganea C; Fendler K; Calinescu O
[Ad] Endereço:Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Frankfurt am Main, Germany.
[Ti] Título:Competition is the basis of the transport mechanism of the NhaB Na+/H+ exchanger from Klebsiella pneumoniae.
[So] Source:PLoS One;12(7):e0182293, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Na+/H+ exchange is essential for survival of all organisms, having a role in the regulation of the intracellular Na+ concentration, pH and cell volume. Furthermore, Na+/H+ exchangers were shown to be involved in the virulence of the bacterium Yersinia pestis, indicating they might be potential targets for novel antibiotic treatments. The model system for Na+/H+ exchangers is the NhaA transporter from Escherichia coli, EcNhaA. Therefore, the general transport mechanism of NhaA exchangers is currently well characterized. However, much less is known about NhaB exchangers, with only a limited number of studies available. The pathogen Klebsiella pneumoniae, which is a major source of nosocomial infection, possesses three electrogenic Na+/H+ exchangers, KpNhaA1, KpNhaA2 and KpNhaB, none of which have been previously investigated. Our aim in this study was to functionally characterize KpNhaB using solid supported membrane-based electrophysiology as the main investigation technique, and thus provide the first electrophysiological investigation of an NhaB Na+/H+ exchanger. We found that NhaB can be described by the same competition-based mechanism that was shown to be valid for electrogenic NhaA and NapA, and for electroneutral NhaP Na+/H+ exchangers. For comparison we also characterized the activity of KpNhaA1 and KpNhaA2 and found that the three exchangers have complementary activity profiles, which is likely a survival advantage for K. pneumoniae when faced with environments of different salinity and pH. This underlines their importance as potential antibiotic drug targets.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Klebsiella pneumoniae/metabolismo
Trocadores de Sódio-Hidrogênio/metabolismo
[Mh] Termos MeSH secundário: Laranja Acridina/metabolismo
Sequência de Aminoácidos
Proteínas de Bactérias/química
Transporte Biológico/efeitos dos fármacos
Membrana Celular/efeitos dos fármacos
Membrana Celular/metabolismo
Escherichia coli/metabolismo
Concentração de Íons de Hidrogênio
Cinética
Lítio/farmacologia
Viabilidade Microbiana/efeitos dos fármacos
Alinhamento de Sequência
Sódio/farmacologia
Trocadores de Sódio-Hidrogênio/química
Especificidade por Substrato/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Sodium-Hydrogen Exchangers); 9FN79X2M3F (Lithium); 9NEZ333N27 (Sodium); F30N4O6XVV (Acridine Orange)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182293


  4 / 2008 MEDLINE  
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[PMID]:28683399
[Au] Autor:Romio KB; Dos Santos KF; da Silva RJ; Pedro MFC; Kalck AS; da Silva Sousa M; Possamai LM; Souto PCS; Silva JR; de Souza NC
[Ad] Endereço:Grupo de Materiais Nanoestruturados, Universidade Federal de Mato Grosso, Barra do Garças, Mato Grosso, Brazil.
[Ti] Título:Incorporation of triclosan and acridine orange into liposomes for evaluating the susceptibility of Candida albicans.
[So] Source:J Photochem Photobiol B;173:514-521, 2017 Aug.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Candida albicans is responsible for many of the infections affecting immunocompromised individuals. Although most C. albicans are susceptible to antifungal drugs, uncontrolled use of these drugs has promoted the development of resistance to current antifungals. The clinical implication of resistant strains has led to the search for safer and more effective drugs as well as alternative approaches, such as controlled drug release using liposomes and photodynamic inactivation (PDI), to eliminate pathogens by combining light and photosensitizers. In this study, we used layer-by-layer (LBL) assembly to immobilize triclosan and acridine orange encapsulated in liposomes and investigated the possibility of controlled release using light. Experiments were carried out to examine the susceptibility of C. albicans to PDI. The effects of laser irradiation were investigated by fluorescence microscopy, atomic force microscopy, and release kinetics. Liposomes were successfully prepared and immobilized using the self-assembly LBL technique. Triclosan was released more quickly when the LBL film was irradiated. The release rate was approximately 40% higher in irradiated films (fluence of 15J/cm ) than in non-irradiated films. The results of the susceptibility experiments and surface morphological analysis indicated that C. albicans cell death is caused by photodynamic inactivation. Liposomes containing triclosan and acridine orange may be useful for inactivating C. albicans using light. Our results lay the foundation for the development of new clinical strategies to control resistant strains.
[Mh] Termos MeSH primário: Laranja Acridina/química
Antifúngicos/farmacologia
Candida albicans/efeitos dos fármacos
Lipossomos/química
Fármacos Fotossensibilizantes/química
Triclosan/química
[Mh] Termos MeSH secundário: Laranja Acridina/metabolismo
Laranja Acridina/farmacologia
Antifúngicos/química
Candida albicans/efeitos da radiação
Liberação Controlada de Fármacos/efeitos da radiação
Lasers
Lipossomos/metabolismo
Microscopia de Força Atômica
Microscopia de Fluorescência
Fármacos Fotossensibilizantes/metabolismo
Fármacos Fotossensibilizantes/farmacologia
Triclosan/metabolismo
Triclosan/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Liposomes); 0 (Photosensitizing Agents); 4NM5039Y5X (Triclosan); F30N4O6XVV (Acridine Orange)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170707
[St] Status:MEDLINE


  5 / 2008 MEDLINE  
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[PMID]:28625706
[Au] Autor:Neeraja M; Lakshmi V; Padmasri C; Padmaja K
[Ad] Endereço:Dept. of Microbiology, Nizam's Institute of Medical Sciences, Hyderabad, Telangana, India.
[Ti] Título:Utility of Acridine Orange staining for detection of bacteria from positive blood cultures.
[So] Source:J Microbiol Methods;139:215-217, 2017 Aug.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The diagnostic performance of AO stain was evaluated for the detection of bacteria and or fungi from positive blood cultures. The sensitivity of Gram stain (GS) was 98.26% while Acridine Orange (AO) stain proved to be more sensitive (100%) with a Positive and Negative Predictive Value of 100% each. The specificity of both the stains was 100%. Overall agreement between the two stains was 98.23% (688/700). The organisms that were missed by GS and positive by AO were Candida species (Sutton, 2006) and Gram negative bacilli (GNB) (Sutton, 2006). Sensitivity of GS was 82.35% and AO was 100% among mixed cultures. Immediate reporting of the results of AO stain would have a significant impact on clinical management of patients with serious blood stream infections.
[Mh] Termos MeSH primário: Laranja Acridina
Bacteriemia/microbiologia
Bactérias/isolamento & purificação
Hemocultura
Corantes
[Mh] Termos MeSH secundário: Bacteriemia/diagnóstico
Hemocultura/normas
Corantes/química
Violeta de Genciana
Bactérias Gram-Negativas/isolamento & purificação
Bactérias Gram-Negativas/metabolismo
Seres Humanos
Fenazinas
Sensibilidade e Especificidade
Coloração e Rotulagem/economia
Leveduras/isolamento & purificação
Leveduras/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coloring Agents); 0 (Gram's stain); 0 (Phenazines); F30N4O6XVV (Acridine Orange); J4Z741D6O5 (Gentian Violet)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE


  6 / 2008 MEDLINE  
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[PMID]:28335646
[Au] Autor:Bragagni M; Carta F; Osman SM; AlOthman Z; Supuran CT
[Ad] Endereço:a Dipartimento Neurofarba, Sezione di Scienze Farmaceutiche e Nutraceutiche , Università degli Studi di Firenze , Sesto Fiorentino , Florence Italy.
[Ti] Título:Synthesis of an acridine orange sulfonamide derivative with potent carbonic anhydrase IX inhibitory action.
[So] Source:J Enzyme Inhib Med Chem;32(1):701-706, 2017 Dec.
[Is] ISSN:1475-6374
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Acridine orange (AO) a fluorescent cationic dye used for the management of human musculoskeletal sarcomas, due to its strong tumoricidal action and accumulation in the acidic environment typical of hypoxic tumors, was used for the preparation of a primary sulfonamide derivative. The rationale behind the drug design is the fact that hypoxic, acidic tumors overexpress carbonic anhydrase (CA, EC 4.2.1.1) isoforms, such as CA IX, which is involved in pH regulation, proliferation, cell migration and invasion, and this enzyme is strongly inhibited by primary sulfonamides. The AO-sulfonamide derivative was indeed a potent, low nanomolar CA IX inhibitor whereas its inhibition of the cytosolic isoforms CA I and II was in the micromolar range. A second transmembrane, tumor-associated isoform, CA XII, was also effectively inhibited by the AO-sulfonamide derivative, making this compound an interesting theranostic agent for the management of hypoxic tumors.
[Mh] Termos MeSH primário: Laranja Acridina/química
Anidrase Carbônica IX/antagonistas & inibidores
Inibidores da Anidrase Carbônica/síntese química
Inibidores da Anidrase Carbônica/farmacologia
Sulfonamidas/química
Sulfonamidas/farmacologia
[Mh] Termos MeSH secundário: Antígenos de Neoplasias/metabolismo
Anidrase Carbônica IX/metabolismo
Inibidores da Anidrase Carbônica/química
Relação Dose-Resposta a Droga
Seres Humanos
Isoenzimas/antagonistas & inibidores
Isoenzimas/metabolismo
Estrutura Molecular
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Neoplasm); 0 (Carbonic Anhydrase Inhibitors); 0 (Isoenzymes); 0 (Sulfonamides); EC 4.2.1.1 (CA9 protein, human); EC 4.2.1.1 (Carbonic Anhydrase IX); F30N4O6XVV (Acridine Orange)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170404
[Lr] Data última revisão:
170404
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE
[do] DOI:10.1080/14756366.2017.1302441


  7 / 2008 MEDLINE  
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[PMID]:28264914
[Au] Autor:Plemel JR; Caprariello AV; Keough MB; Henry TJ; Tsutsui S; Chu TH; Schenk GJ; Klaver R; Yong VW; Stys PK
[Ad] Endereço:Department of Clinical Neurosciences and the Hotchkiss Brain Institute, University of Calgary, Calgary, AB T2N 4N1, Canada.
[Ti] Título:Unique spectral signatures of the nucleic acid dye acridine orange can distinguish cell death by apoptosis and necroptosis.
[So] Source:J Cell Biol;216(4):1163-1181, 2017 Apr 03.
[Is] ISSN:1540-8140
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cellular injury and death are ubiquitous features of disease, yet tools to detect them are limited and insensitive to subtle pathological changes. Acridine orange (AO), a nucleic acid dye with unique spectral properties, enables real-time measurement of RNA and DNA as proxies for cell viability during exposure to various noxious stimuli. This tool illuminates spectral signatures unique to various modes of cell death, such as cells undergoing apoptosis versus necrosis/necroptosis. This new approach also shows that cellular RNA decreases during necrotic, necroptotic, and apoptotic cell death caused by demyelinating, ischemic, and traumatic injuries, implying its involvement in a wide spectrum of tissue pathologies. Furthermore, cells with pathologically low levels of cytoplasmic RNA are detected earlier and in higher numbers than with standard markers including TdT-mediated dUTP biotin nick-end labeling and cleaved caspase 3 immunofluorescence. Our technique highlights AO-labeled cytoplasmic RNA as an important early marker of cellular injury and a sensitive indicator of various modes of cell death in a range of experimental models.
[Mh] Termos MeSH primário: Laranja Acridina/metabolismo
Apoptose/fisiologia
Morte Celular/fisiologia
Necrose/patologia
Ácidos Nucleicos/metabolismo
[Mh] Termos MeSH secundário: Animais
Caspase 3/metabolismo
Linhagem Celular
Nucleotídeos de Desoxiuracil/metabolismo
Seres Humanos
Marcação In Situ das Extremidades Cortadas/métodos
Camundongos Endogâmicos C57BL
Necrose/metabolismo
RNA/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Deoxyuracil Nucleotides); 0 (Nucleic Acids); 1173-82-6 (deoxyuridine triphosphate); 63231-63-0 (RNA); EC 3.4.22.- (Caspase 3); F30N4O6XVV (Acridine Orange)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170308
[St] Status:MEDLINE
[do] DOI:10.1083/jcb.201602028


  8 / 2008 MEDLINE  
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[PMID]:28262028
[Au] Autor:Iessi E; Logozzi M; Lugini L; Azzarito T; Federici C; Spugnini EP; Mizzoni D; Di Raimo R; Angelini DF; Battistini L; Cecchetti S; Fais S
[Ad] Endereço:a Anti-Tumour Drugs Section, Department of Drug Research and Medicines Evaluation , National Institute of Health , Rome , Italy.
[Ti] Título:Acridine Orange/exosomes increase the delivery and the effectiveness of Acridine Orange in human melanoma cells: A new prototype for theranostics of tumors.
[So] Source:J Enzyme Inhib Med Chem;32(1):648-657, 2017 Dec.
[Is] ISSN:1475-6374
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Specifically targeted drug delivery systems with low immunogenicity and toxicity are deemed to increase efficacy of cancer chemotherapy. Acridine Orange (AO) is an acidophilic dye with a strong tumoricidal action following excitation with a light source at 466 nm. However, to date the clinical use of AO is limited by the potential side effects elicited by systemic administration. The endogenous nanocarrier exosomes have been recently introduced as a natural delivery system for therapeutic molecules. In this article, we show the outcome of the administration to human melanoma cells of AO charged Exosomes (Exo-AO), in both monolayer and spheroid models. The results showed an extended drug delivery time of Exo-AO to melanoma cells as compared to the free AO, improving the cytotoxicity of AO. This study shows that Exo-AO have a great potential for a real exploitation as a new theranostic approach against tumors based on AO delivered through the exosomes.
[Mh] Termos MeSH primário: Laranja Acridina/química
Sistemas de Liberação de Medicamentos
Exossomos
Melanoma/tratamento farmacológico
Nanomedicina Teranóstica
[Mh] Termos MeSH secundário: Laranja Acridina/uso terapêutico
Antineoplásicos/química
Antineoplásicos/uso terapêutico
Linhagem Celular Tumoral
Citometria de Fluxo
Seres Humanos
Concentração de Íons de Hidrogênio
Microscopia Confocal
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); F30N4O6XVV (Acridine Orange)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170425
[Lr] Data última revisão:
170425
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170307
[St] Status:MEDLINE
[do] DOI:10.1080/14756366.2017.1292263


  9 / 2008 MEDLINE  
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[PMID]:28183660
[Au] Autor:Ramachandran R; Krishnaraj C; Sivakumar AS; Prasannakumar P; Abhay Kumar VK; Shim KS; Song CG; Yun SI
[Ad] Endereço:Centre for Advanced Studies in Botany, School of Life Sciences, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India.
[Ti] Título:Anticancer activity of biologically synthesized silver and gold nanoparticles on mouse myoblast cancer cells and their toxicity against embryonic zebrafish.
[So] Source:Mater Sci Eng C Mater Biol Appl;73:674-683, 2017 Apr 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to evaluate the anticancer activity of bioinspired silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) against mouse myoblast cancer cells (C C ). Both AgNPs and AuNPs were biologically synthesized using Spinacia oleracea Linn., aqueous leaves extract. UV-Vis. spectrophotometer, high resolution-transmission electron microscopy (HR-TEM), field emission-scanning electron microscopy (FE-SEM) and X-ray diffraction (XRD) studies supported the successful synthesis of AgNPs and AuNPs. Both these NPs have shown cytotoxicity against C C cells even at very low concentration (5µg/mL). Acridine orange/Ethidium bromide (AO/EB) dual staining confirmed the apoptotic morphological features. The levels of caspase enzymes (caspase-3 and caspase-7) were significantly up-regulated in NPs treated myoblast cells than the plant extract. Furthermore, in zebrafish embryo toxicity study, AgNPs showed 100% mortality at 3µg/mL concentration while AuNPs exhibited the same at much higher concentration (300mg/mL). Taken together, these results provide a preliminary guidance for the development of biomaterials based drugs to fight against the fatal diseases for example cancer.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Embrião não Mamífero/efeitos dos fármacos
Ouro/farmacologia
Nanopartículas Metálicas/toxicidade
Mioblastos/patologia
Prata/farmacologia
Testes de Toxicidade
Peixe-Zebra/embriologia
[Mh] Termos MeSH secundário: Laranja Acridina/metabolismo
Animais
Apoptose/efeitos dos fármacos
Caspases/metabolismo
Linhagem Celular Tumoral
Forma Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Embrião não Mamífero/anormalidades
Etídio/metabolismo
Nanopartículas Metálicas/ultraestrutura
Camundongos
Mioblastos/efeitos dos fármacos
Técnicas Fotoacústicas
Extratos Vegetais/farmacologia
Folhas de Planta/química
Spinacia oleracea/química
Coloração e Rotulagem
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Plant Extracts); 3M4G523W1G (Silver); 7440-57-5 (Gold); EC 3.4.22.- (Caspases); EN464416SI (Ethidium); F30N4O6XVV (Acridine Orange)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170903
[Lr] Data última revisão:
170903
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE


  10 / 2008 MEDLINE  
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[PMID]:28130157
[Au] Autor:Amado AM; Pazin WM; Ito AS; Kuzmin VA; Borissevitch IE
[Ad] Endereço:Departamento de Física, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes 3900, Vila Monte Alegre, CEP 14040-901 Ribeirão Preto, SP, Brazil.
[Ti] Título:Acridine orange interaction with DNA: Effect of ionic strength.
[So] Source:Biochim Biophys Acta;1861(4):900-909, 2017 04.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The study of acridine orange (AO) spectral characteristics and the quenching of its singlet and triplet excited states by TEMPO radical at its binding to DNA in the function of the DNA concentration and in the absence and presence of NaCl is reported. METHODS: The study was performed using steady-state and time resolved optical absorption and florescence, fluorescence correlation spectroscopy and resonant light scattering techniques. RESULTS: The presence of different species in equilibrium: AO monomers and aggregates bound to DNA, has been demonstrated, their relative content depending on the DNA and the AO concentrations. At high DNA concentration the AO monomers are protected against the contact with other molecules, thus reducing the AO excited state quenching. The addition of NaCl reduces the AO binding constant to DNA, thus reducing the AO and DNA aggregation. CONCLUSIONS: The interaction of AO with DNA is a complex process, including aggregation and disaggregation of both components. This modifies the AO excited state characteristics and AO accessibility to other molecules. The salt reduces the DNA effects on the AO excited state characteristics thus attenuating its effects on the AO efficacy in applications. GENERAL SIGNIFICANCE: This study demonstrates that the interaction of photosensitizers with DNA, depending on their relative concentrations, can both decrease and increase the photosensitizer efficacy in applications. The salt is able to attenuate these effects.
[Mh] Termos MeSH primário: Laranja Acridina/química
DNA/química
[Mh] Termos MeSH secundário: Concentração Osmolar
Cloreto de Sódio/química
Espectrometria de Fluorescência/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
451W47IQ8X (Sodium Chloride); 9007-49-2 (DNA); F30N4O6XVV (Acridine Orange)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170129
[St] Status:MEDLINE



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