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[PMID]:28470518
[Au] Autor:Chen D; Eyupoglu IY; Savaskan N
[Ad] Endereço:Translational Cell Biology and Neurooncology Laboratory of the Universitätsklinikum Erlangen (UKER), Friedrich-Alexander University of Erlangen - Nürnberg (FAU), and Department of Neurosurgery of the Universitätsklinikum Erlangen, Universitätsklinikum Erlangen (UKER), Friedrich-Alexander University
[Ti] Título:Ferroptosis and Cell Death Analysis by Flow Cytometry.
[So] Source:Methods Mol Biol;1601:71-77, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cell death and its recently discovered regulated form ferroptosis are characterized by distinct morphological, electrophysiological, and pharmacological features. In particular ferroptosis can be induced by experimental compounds and clinical drugs (i.e., erastin, sulfasalazine, sorafenib, and artesunate) in various cell types and cancer cells. Pharmacologically, this cell death process can be inhibited by iron chelators and lipid peroxidation inhibitors. Relevance of this specific cell death form has been found in different pathological conditions such as cancer, neurotoxicity, neurodegeneration, and ischemia. Distinguishing cell viability and cell death is essential for experimental and clinical applications and a key component in flow cytometry experiments. Dead cells can compromise the integrity of the data by nonspecific binding of antibodies and dyes. Therefore it is essential that dead cells are robustly and reproducibly identified and characterized by means of cytometry application. Here we describe a procedure to detect and quantify cell death and its specific form ferroptosis based on standard flow cytometry techniques.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Citometria de Fluxo/métodos
Ferro/metabolismo
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/farmacologia
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Dactinomicina/análogos & derivados
Dactinomicina/química
Seres Humanos
Indicadores e Reagentes/química
Necrose
Niacinamida/análogos & derivados
Niacinamida/farmacologia
Compostos de Fenilureia/farmacologia
Piperazinas/farmacologia
Propídio/química
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Indicators and Reagents); 0 (Phenylurea Compounds); 0 (Piperazines); 0 (erastin); 1CC1JFE158 (Dactinomycin); 25X51I8RD4 (Niacinamide); 36015-30-2 (Propidium); 7240-37-1 (7-aminoactinomycin D); 9ZOQ3TZI87 (sorafenib); E1UOL152H7 (Iron)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6960-9_6


  2 / 17436 MEDLINE  
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[PMID]:29390471
[Au] Autor:Niu G; Yuan LJ; Gong FQ; Yang J; Zhu CX; Shen HW
[Ad] Endereço:Department of Gynecology and Obstetrics.
[Ti] Título:Early pregnancy following multidrug regimen chemotherapy in a gestational trophoblastic neoplasia patient: A case report.
[So] Source:Medicine (Baltimore);96(51):e9221, 2017 Dec.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RATIONALE: Gestational trophoblastic neoplasia is a group of rare tumors that can be cured using chemotherapy. The use of artificial contraception for at least 1 year is recommended not only due to the high recurrence rate in the first year after treatment, but also because of the unclear genetic toxic effects of multidrug regimen chemotherapy on reproductive cells. There is no consensus about the contraception duration, but most patients want to have children. PATIENT CONCERNS: This case involved a 33-year-old female suffering from gestational trophoblastic neoplasia and 5-fluorouracil + actinomycin-D chemotherapy. She became pregnant 1 month after finishing the chemotherapy. DIAGNOSIS: Gestational trophoblastic neoplasia. INTERVENTIONS: No treatment during pregnancy. OUTCOMES: The patient had a full-term normal delivery, and the baby showed normal development and growth after a follow-up of 48 months. LESSONS: Pregnancy soon after chemotherapy can be viable with rigorous prenatal care.
[Mh] Termos MeSH primário: Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico
Doença Trofoblástica Gestacional/tratamento farmacológico
Complicações Neoplásicas na Gravidez/tratamento farmacológico
Resultado da Gravidez
[Mh] Termos MeSH secundário: Adulto
Gonadotropina Coriônica/sangue
Dactinomicina/administração & dosagem
Relação Dose-Resposta a Droga
Esquema de Medicação
Feminino
Desenvolvimento Fetal/fisiologia
Fluoruracila/administração & dosagem
Idade Gestacional
Doença Trofoblástica Gestacional/diagnóstico
Seres Humanos
Saúde do Lactente
Recém-Nascido
Masculino
Gravidez
Complicações Neoplásicas na Gravidez/diagnóstico
Cuidado Pré-Natal/métodos
Nascimento a Termo
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Chorionic Gonadotropin); 1CC1JFE158 (Dactinomycin); U3P01618RT (Fluorouracil)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180203
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000009221


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[PMID]:29277804
[Au] Autor:Bacalbasa N; Balescu I; Brasoveanu V; Anca AF
[Ad] Endereço:"Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania.
[Ti] Título:Debulking Surgery for Pelvic Recurrence After Surgically-treated Tubal Gestational Choriocarcinoma - A Case Report and Literature Review.
[So] Source:Anticancer Res;38(1):423-426, 2018 01.
[Is] ISSN:1791-7530
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Gestational choriocarcinomas are very rare malignancies, with only few cases being reported so far. Moreover, the presence of pelvic recurrences after surgically-treated gestational choriocarcinomas is even scarcer situations. We present the case of a 19-year-old patient who was initially submitted to surgery with preoperative diagnosis of ectopic pregnancy. At that moment a left salpingectomy was performed, and the histopathological studies revealed the presence of a left tubal gestational choriocarcinoma. The patient was submitted to adjuvant chemotherapy with methotrexate and dactinomycin. However, six months later she was diagnosed with a pelvic recurrence so she was resubmitted to surgery, debulking to no residual disease being successfully performed. The histopathological studies confirmed the presence of a recurrent tumor with gestational choriocarcinoma structure.
[Mh] Termos MeSH primário: Coriocarcinoma/cirurgia
Procedimentos Cirúrgicos de Citorredução/métodos
Neoplasias das Tubas Uterinas/cirurgia
Doença Trofoblástica Gestacional/cirurgia
Complicações Neoplásicas na Gravidez/cirurgia
Salpingectomia
[Mh] Termos MeSH secundário: Adulto
Antibióticos Antineoplásicos/uso terapêutico
Antimetabólitos Antineoplásicos/uso terapêutico
Coriocarcinoma/tratamento farmacológico
Dactinomicina/uso terapêutico
Feminino
Seres Humanos
Metotrexato/uso terapêutico
Recidiva Local de Neoplasia
Gravidez
Gravidez Ectópica/diagnóstico
Adulto Jovem
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibiotics, Antineoplastic); 0 (Antimetabolites, Antineoplastic); 1CC1JFE158 (Dactinomycin); YL5FZ2Y5U1 (Methotrexate)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171227
[St] Status:MEDLINE


  4 / 17436 MEDLINE  
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[PMID]:29190286
[Au] Autor:Tchelidze P; Benassarou A; Kaplan H; O'Donohue MF; Lucas L; Terryn C; Rusishvili L; Mosidze G; Lalun N; Ploton D
[Ad] Endereço:Faculty of Exact and Life Sciences, Department of Morphology, Tbilisi State University, Tbilisi, Georgia.
[Ti] Título:Nucleolar sub-compartments in motion during rRNA synthesis inhibition: Contraction of nucleolar condensed chromatin and gathering of fibrillar centers are concomitant.
[So] Source:PLoS One;12(11):e0187977, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The nucleolus produces the large polycistronic transcript (47S precursor) containing the 18S, 5.8S and 28S rRNA sequences and hosts most of the nuclear steps of pre-rRNA processing. Among numerous components it contains condensed chromatin and active rRNA genes which adopt a more accessible conformation. For this reason, it is a paradigm of chromosome territory organization. Active rRNA genes are clustered within several fibrillar centers (FCs), in which they are maintained in an open configuration by Upstream Binding Factor (UBF) molecules. Here, we used the reproducible reorganization of nucleolar components induced by the inhibition of rRNA synthesis by Actinomycin D (AMD) to address the steps of the spatiotemporal reorganization of FCs and nucleolar condensed chromatin. To reach that goal, we used two complementary approaches: i) time-lapse confocal imaging of cells expressing one or several GFP-tagged proteins (fibrillarin, UBF, histone H2B) and ii) ultrastructural identification of nucleolar components involved in the reorganization. Data obtained by time lapse confocal microscopy were analyzed through detailed 3D imaging. This allowed us to demonstrate that AMD treatment induces no fusion and no change in the relative position of the different nucleoli contained in one nucleus. In contrast, for each nucleolus, we observed step by step gathering and fusion of both FCs and nucleolar condensed chromatin. To analyze the reorganization of FCs and condensed chromatin at a higher resolution, we performed correlative light and electron microscopy electron microscopy (CLEM) imaging of the same cells. We demonstrated that threads of intranucleolar condensed chromatin are localized in a complex 3D network of vacuoles. Upon AMD treatment, these structures coalesce before migrating toward the perinucleolar condensed chromatin, to which they finally fuse. During their migration, FCs, which are all linked to ICC, are pulled by the latter to gather as caps disposed at the periphery of nucleoli.
[Mh] Termos MeSH primário: Compartimento Celular
Nucléolo Celular/metabolismo
Cromatina/metabolismo
RNA Ribossômico/antagonistas & inibidores
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Dactinomicina/farmacologia
Seres Humanos
Microscopia Eletrônica de Transmissão
RNA Ribossômico/biossíntese
RNA Ribossômico/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromatin); 0 (RNA, Ribosomal); 1CC1JFE158 (Dactinomycin)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0187977


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[PMID]:28450600
[Au] Autor:Bennett JW; Eveleigh D; Goodman RM
[Ad] Endereço:School of Environmental and Biological Sciences, Rutgers University, New Brunswick, NJ 08901, USA.
[Ti] Título:H. Boyd Woodruff (1917-2017).
[So] Source:Science;356(6336):381, 2017 Apr 28.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Antibacterianos/história
Indústria Farmacêutica/história
Microbiologia Industrial/história
[Mh] Termos MeSH secundário: Actinomyces/crescimento & desenvolvimento
Actinomyces/metabolismo
Dactinomicina/metabolismo
História do Século XX
História do Século XXI
Estados Unidos
[Pt] Tipo de publicação:BIOGRAPHY; HISTORICAL ARTICLE; JOURNAL ARTICLE
[Ps] Nome de pessoa como assunto:Woodruff HB
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 1CC1JFE158 (Dactinomycin)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1126/science.aan3952


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[PMID]:28461032
[Au] Autor:Kong Y; Yang J; Jiang F; Zhao J; Ren T; Li J; Wang X; Feng F; Wan X; Xiang Y
[Ad] Endereço:Departments of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Shuaifuyuan, Wangfujing, Dongcheng District, 100730 Beijing, PR China.
[Ti] Título:Clinical characteristics and prognosis of ultra high-risk gestational trophoblastic neoplasia patients: A retrospective cohort study.
[So] Source:Gynecol Oncol;146(1):81-86, 2017 07.
[Is] ISSN:1095-6859
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: The gestational trophoblastic neoplasia (GTN) patients with the International Federation of Gynecology and Obstetrics (FIGO) score≥12 are defined as ultra high-risk GTN. This study aims to investigate the clinical characteristics, the treatment efficiency, and the prognosis of ultra high-risk GTN patients. METHODS: Between January 2002 and December 2015, medical record data of 143 GTN patients with FIGO score≥12 at Peking Union Medical College Hospital (PUMCH) were reviewed. Ratios were compared using chi-square test, and prognostic risk factors were analyzed by univariate analysis and multivariate analysis. RESULTS: Among the 143 ultra high-risk GTN patients, 94 (65.7%) patients had achieved complete remission and 15.9% (15/94) patients relapsed after complete remission. The 5-year overall survival (OS) rate of the entire cohort approached 67.9%. The results of the multivariate analysis revealed that non-molar antecedent pregnancy [Relative risk (RR) 4.689, 95% CI 1.448-15.189, P=0.010], brain metastases (RR 2.280, 95% CI 1.248-4.163, P=0.007), previous failed multiagent chemotherapy (RR 5.345, 95% CI 2.222-12.857, P=0.000) and surgery (RR 0.336, 95% CI 0.177-0.641, P=0.001) all had influence on the prognosis of ultra high-risk GTN patients. CONCLUSIONS: GTN patients with FIGO score≥12 have a poor prognosis. More emphasis should be placed on non-molar antecedent pregnancy, brain metastases, and previous multiagent chemotherapy failure. Moreover, salvage surgery may improve the prognosis. Floxuridine-based multiagent chemotherapy is effective with manageable toxicity for ultra high-risk GTN patients.
[Mh] Termos MeSH primário: Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico
Doença Trofoblástica Gestacional/diagnóstico
Doença Trofoblástica Gestacional/tratamento farmacológico
[Mh] Termos MeSH secundário: Adolescente
Adulto
Estudos de Coortes
Dactinomicina/administração & dosagem
Etoposídeo/administração & dosagem
Feminino
Floxuridina/administração & dosagem
Seres Humanos
Meia-Idade
Gravidez
Prognóstico
Estudos Retrospectivos
Fatores de Risco
Terapia de Salvação
Vincristina/administração & dosagem
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
039LU44I5M (Floxuridine); 1CC1JFE158 (Dactinomycin); 5J49Q6B70F (Vincristine); 6PLQ3CP4P3 (Etoposide)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171211
[Lr] Data última revisão:
171211
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


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[PMID]:28843054
[Au] Autor:Furtwängler R; Kager L; Melchior P; Rübe C; Ebinger M; Nourkami-Tutdibi N; Niggli F; Warmann S; Hubertus J; Amman G; Leuschner I; Vokuhl C; Graf N; Frühwald MC
[Ad] Endereço:Department of Pediatric Hematology and Oncology, Saarland University Hospital, Homburg/Saar, Germany.
[Ti] Título:High-dose treatment for malignant rhabdoid tumor of the kidney: No evidence for improved survival-The Gesellschaft für Pädiatrische Onkologie und Hämatologie (GPOH) experience.
[So] Source:Pediatr Blood Cancer;65(1), 2018 Jan.
[Is] ISSN:1545-5017
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Malignant rhabdoid tumor of the kidney (MRTK) is the most aggressive childhood renal tumor with overall survival (OS) rates ranging from 22% to 42%. Whether high-dose chemotherapy with autologous stem-cell transplantation (HDSCT) in an intensive first-line treatment offers additional benefit is an ongoing discussion. METHODS: A retrospective analysis of all 58 patients with MRTK from Austria, Switzerland, and Germany treated in the framework of consecutive, prospective renal/rhabdoid tumor studies SIOP9/GPO, SIOP93-01/GPOH (where SIOP is International Society of Pediatric Oncology and GPOH is German Society of Pediatric Oncology and Hematology), SIOP2001/GPOH, and European Rhabdoid Tumor Registry from 1991 to 2014. RESULTS: Median age at diagnosis was 11 months. Fifty percent of patients had metastases or multifocal disease at diagnosis (Stage IV). Local stage distribution was as follows: not done/I/II/III-1/6/11/40. Fifteen (26%) patients underwent upfront surgery. Thirty-seven (64%) patients achieved a complete remission, 17 (29%) relapsed, 34 (59%) died of disease progression, and two (3%) died of treatment-related complication. Mean time to the first event was 3.5 months. Two-year EFS/OS (where EFS is event-free survival) for the whole group was 37 ± 6%/38 ± 6%. Metastases/multifocal disease, younger age, and local stage III were associated with significantly inferior survival. Eleven (19%) patients underwent HDSCT (carboplatin + thiotepa, n = 6; carboplatin + etoposide + melphalan, n = 4; others, n = 1); 2-year OS in this group was 60 ± 15% compared to 34 ± 8% in the non-HDSCT group (P = 0.064). However, the time needed from radiologic to histologic diagnosis, stem-cell harvest, and HDSCT must also be taken into account to avoid selection bias by excluding the highest risk group with early progression (<90 days). Thus, 2-year EFS only for patients without progression until day 90 was 60 ± 16% consolidated by HDSCT compared to 62 ± 11% without (P = 0.8). CONCLUSION: Our retrospective analysis suggests comparable outcomes for patients with and without HDSCT, if adjusted for early disease progression.
[Mh] Termos MeSH primário: Protocolos de Quimioterapia Combinada Antineoplásica/administração & dosagem
Neoplasias Renais/tratamento farmacológico
Neoplasias Renais/mortalidade
Sistema de Registros
Tumor Rabdoide/tratamento farmacológico
Tumor Rabdoide/mortalidade
[Mh] Termos MeSH secundário: Adolescente
Fatores Etários
Criança
Pré-Escolar
Dactinomicina/administração & dosagem
Intervalo Livre de Doença
Feminino
Seres Humanos
Lactente
Recém-Nascido
Masculino
Estudos Prospectivos
Taxa de Sobrevida
Vincristina/administração & dosagem
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE; MULTICENTER STUDY
[Nm] Nome de substância:
1CC1JFE158 (Dactinomycin); 5J49Q6B70F (Vincristine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171129
[Lr] Data última revisão:
171129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170827
[St] Status:MEDLINE
[do] DOI:10.1002/pbc.26746


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[PMID]:28834127
[Au] Autor:Kinoshita M; Yamada A; Sawa D; Kamimura S; Miyachi M; Moritake H
[Ad] Endereço:Division of Pediatrics, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
[Ti] Título:Successful treatment of metastatic alveolar rhabdomyosarcoma with MGMT gene promoter methylation by temozolomide-based combination chemotherapy.
[So] Source:Pediatr Blood Cancer;65(1), 2018 Jan.
[Is] ISSN:1545-5017
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A 3-year-old male presented with a large retroperitoneal mass and multiple metastases. Biopsy results suggested alveolar rhabdomyosarcoma bearing a methylated O6-methylguanine-DNA methyltransferase (MGMT) gene promoter. Serum microRNA-206 levels were elevated and remained high after three cycles of vincristine, dactinomycin, and cyclophosphamide (VAC). Replacement of vincristine, irinotecan, and temozolomide (VIT) for VAC induced a marked tumor reduction and normalization of the miR-206 levels. The patient completed 14 cycles of VIT with local radiotherapy and has been in remission for 31 months. Temozolomide could be effective for tumors with a methylated MGMT gene promoter. Individualized therapy is warranted for such patients.
[Mh] Termos MeSH primário: Protocolos de Quimioterapia Combinada Antineoplásica/administração & dosagem
Quimiorradioterapia
Metilação de DNA
Metilases de Modificação do DNA/metabolismo
Enzimas Reparadoras do DNA/metabolismo
DNA de Neoplasias/metabolismo
Regiões Promotoras Genéticas
Rabdomiossarcoma Alveolar
Proteínas Supressoras de Tumor/metabolismo
[Mh] Termos MeSH secundário: Camptotecina/administração & dosagem
Camptotecina/análogos & derivados
Pré-Escolar
Ciclofosfamida/administração & dosagem
Dacarbazina/administração & dosagem
Dacarbazina/análogos & derivados
Dactinomicina/administração & dosagem
Seres Humanos
Masculino
MicroRNAs/metabolismo
Metástase Neoplásica
RNA Neoplásico/metabolismo
Rabdomiossarcoma Alveolar/metabolismo
Rabdomiossarcoma Alveolar/patologia
Rabdomiossarcoma Alveolar/terapia
Vincristina/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Neoplasm); 0 (MIRN206 microRNA, human); 0 (MicroRNAs); 0 (RNA, Neoplasm); 0 (Tumor Suppressor Proteins); 1CC1JFE158 (Dactinomycin); 5J49Q6B70F (Vincristine); 7673326042 (irinotecan); 7GR28W0FJI (Dacarbazine); 8N3DW7272P (Cyclophosphamide); EC 2.1.1.- (DNA Modification Methylases); EC 2.1.1.63 (MGMT protein, human); EC 6.5.1.- (DNA Repair Enzymes); XT3Z54Z28A (Camptothecin); YF1K15M17Y (temozolomide)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171129
[Lr] Data última revisão:
171129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170824
[St] Status:MEDLINE
[do] DOI:10.1002/pbc.26750


  9 / 17436 MEDLINE  
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[PMID]:28910374
[Au] Autor:Fehrholz M; Seidenspinner S; Kunzmann S
[Ad] Endereço:University Children's Hospital, University of Wuerzburg, Wuerzburg, Germany.
[Ti] Título:Expression of surfactant protein B is dependent on cell density in H441 lung epithelial cells.
[So] Source:PLoS One;12(9):e0184556, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Expression of surfactant protein (SP)-B, which assures the structural stability of the pulmonary surfactant film, is influenced by various stimuli, including glucocorticoids; however, the role that cell-cell contact plays in SP-B transcription remains unknown. The aim of the current study was to investigate the impact of cell-cell contact on SP-B mRNA and mature SP-B expression in the lung epithelial cell line H441. METHODS: Different quantities of H441 cells per growth area were either left untreated or incubated with dexamethasone. The expression of SP-B, SP-B transcription factors, and tight junction proteins were determined by qPCR and immunoblotting. The influence of cell density on SP-B mRNA stability was investigated using the transcription inhibitor actinomycin D. RESULTS: SP-B mRNA and mature SP-B expression levels were significantly elevated in untreated and dexamethasone-treated H441 cells with increasing cell density. High cell density as a sole stimulus was found to barely have an impact on SP-B transcription factor and tight junction mRNA levels, while its stimulatory ability on SP-B mRNA expression could be mimicked using SP-B-negative cells. SP-B mRNA stability was significantly increased in high-density cells, but not by dexamethasone alone. CONCLUSION: SP-B expression in H441 cells is dependent on cell-cell contact, which increases mRNA stability and thereby potentiates the glucocorticoid-mediated induction of transcription. Loss of cell integrity might contribute to reduced SP-B secretion in damaged lung cells via downregulation of SP-B transcription. Cell density-mediated effects should thus receive greater attention in future cell culture-based research.
[Mh] Termos MeSH primário: Células Epiteliais/citologia
Pulmão/citologia
Proteína B Associada a Surfactante Pulmonar/genética
Proteínas de Junções Íntimas/genética
[Mh] Termos MeSH secundário: Células A549
Contagem de Células
Linhagem Celular
Dactinomicina/farmacologia
Dexametasona/farmacologia
Regulação para Baixo
Células Epiteliais/metabolismo
Seres Humanos
Pulmão/metabolismo
Proteína B Associada a Surfactante Pulmonar/metabolismo
Estabilidade de RNA
RNA Mensageiro/química
RNA Mensageiro/metabolismo
Proteínas de Junções Íntimas/metabolismo
Transcrição Genética/efeitos dos fármacos
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pulmonary Surfactant-Associated Protein B); 0 (RNA, Messenger); 0 (Tight Junction Proteins); 1CC1JFE158 (Dactinomycin); 7S5I7G3JQL (Dexamethasone)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170915
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184556


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[PMID]:28885339
[Au] Autor:Yang M; Peng L
[Ad] Endereço:Department of Respiratory and Critical Care Medicine, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
[Ti] Título:Role of chemotherapy and thrombolysis in treatment of choriocarcinoma accompanied with pulmonary embolism: A case report with literature review.
[So] Source:Medicine (Baltimore);96(36):e7866, 2017 Sep.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RATIONALE: Choriocarcinoma accompanied with pulmonary embolism (PE) is rare and difficult to diagnose and treat. There are about 25 cases reported in literature, which presented variable clinical characteristics and prognosis. PATIENT CONCERNS: We reported a case of choriocarcinoma presenting PE as the first manifestation in a 26-year-old Chinese female. DIAGNOSES: Four months before her admission to our hospital, she presented to a local hospital with respiratory manifestations, and was first diagnosed as bronchitis and treated with antibiotics without improvement, and subsequently suspected of having pulmonary tuberculosis and receive anti-tuberculosis therapy, with no response. Upon admission to our hospital, spiral computed tomography-pulmonary angiography revealed complete occlusion of right pulmonary artery, leading to PE. She received thrombolysis and anticoagulant therapy, without improvement. Further interrogation of the patient revealed a history of irregular vaginal bleeding and multiple pregnancies and abortions. Following the laboratory report of a significantly elevated level of serum beta-human chorionic gonadotropin (ß-HCG) combined with other clinical and laboratory findings, a diagnosis of choriocarcinoma accompanied with PE was established based on the criteria formulated by the International Federation of Gynecology and Obstetrics and guideline of European Society for Medical Oncology. INTERVENTIONS: With 1 cycle of chemotherapy with etoposide-methotrexateactinomycin D-cyclophosphamide-vincristine (EMA-CO), her dyspnea and other symptoms improved, with a significant decrease in the serum ß-HCG level and pulmonary artery pressure. OUTCOMES: Unfortunately, she showed bone marrow inhibition, could not continue further chemotherapy, and finally died after discharging. We reviewed 25 similar cases in the literature, and found that all 17 cases receiving chemotherapy showed complete recovery while 6 of 8 cases without chemotherapy died during hospitalization. LESSONS: This case report illustrates the challenges in diagnosis of choriocarcinoma presenting with respiratory manifestations, and highlights the importance of early diagnosis and timely appropriate chemotherapy in management of this disease.
[Mh] Termos MeSH primário: Anticoagulantes/uso terapêutico
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico
Coriocarcinoma/tratamento farmacológico
Embolia Pulmonar/tratamento farmacológico
Neoplasias Uterinas/tratamento farmacológico
[Mh] Termos MeSH secundário: Adulto
Coriocarcinoma/complicações
Coriocarcinoma/diagnóstico
Gonadotropina Coriônica Humana Subunidade beta/sangue
Ciclofosfamida/uso terapêutico
Dactinomicina/uso terapêutico
Etoposídeo/uso terapêutico
Feminino
Seres Humanos
Metotrexato/uso terapêutico
Gravidez
Embolia Pulmonar/etiologia
Neoplasias Uterinas/complicações
Neoplasias Uterinas/diagnóstico
Vincristina/uso terapêutico
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anticoagulants); 0 (Chorionic Gonadotropin, beta Subunit, Human); 1CC1JFE158 (Dactinomycin); 5J49Q6B70F (Vincristine); 6PLQ3CP4P3 (Etoposide); 8N3DW7272P (Cyclophosphamide); YL5FZ2Y5U1 (Methotrexate)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000007866



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