Base de dados : MEDLINE
Pesquisa : D03.633.300.633.416 [Categoria DeCS]
Referências encontradas : 4551 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 456 ir para página                         

  1 / 4551 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29317622
[Au] Autor:Zwama M; Yamasaki S; Nakashima R; Sakurai K; Nishino K; Yamaguchi A
[Ad] Endereço:Laboratory of Cell Membrane Structural Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka, 567-0047, Japan.
[Ti] Título:Multiple entry pathways within the efflux transporter AcrB contribute to multidrug recognition.
[So] Source:Nat Commun;9(1):124, 2018 01 09.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AcrB is the major multidrug exporter in Escherichia coli. Although several substrate-entrances have been identified, the specificity of these various transport paths remains unclear. Here we present evidence for a substrate channel (channel 3)  from the central cavity of the AcrB trimer, which is connected directly to the deep pocket without first passing the switch-loop and the proximal pocket . Planar aromatic cations, such as ethidium, prefer channel 3 to channels 1 and 2. The efflux through channel 3 increases by targeted mutations and is not in competition with the export of drugs such as minocycline and erythromycin through channels 1 and 2. A switch-loop mutant, in which the pathway from the proximal to the deep pocket is hindered, can export only channel 3-utilizing drugs. The usage of multiple entrances thus contributes to the recognition and transport of a wide range of drugs with different physicochemical properties.
[Mh] Termos MeSH primário: Farmacorresistência Bacteriana Múltipla/genética
Proteínas de Escherichia coli/genética
Proteínas Associadas à Resistência a Múltiplos Medicamentos/genética
Transdução de Sinais/genética
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Escherichia coli/efeitos dos fármacos
Escherichia coli/genética
Escherichia coli/metabolismo
Proteínas de Escherichia coli/química
Proteínas de Escherichia coli/metabolismo
Etídio/química
Etídio/metabolismo
Testes de Sensibilidade Microbiana
Modelos Moleculares
Proteínas Associadas à Resistência a Múltiplos Medicamentos/química
Proteínas Associadas à Resistência a Múltiplos Medicamentos/metabolismo
Mutação
Domínios Proteicos
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (AcrB protein, E coli); 0 (Anti-Bacterial Agents); 0 (Escherichia coli Proteins); 0 (Multidrug Resistance-Associated Proteins); EN464416SI (Ethidium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02493-1


  2 / 4551 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29325850
[Au] Autor:Nejat Dehkordi M; Akerman B
[Ad] Endereço:Department of Basic Science, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran; Department of Chemical and Biological Engineering, Chalmers University of Technology, SE-412 96, Gothenburg, Sweden. Electronic address: m.nejat@iaushk.ac.ir.
[Ti] Título:Interaction of DNA with water soluble complex of Nickle and formation of DNA cross-links.
[So] Source:Chem Biol Interact;282:55-62, 2018 Feb 25.
[Is] ISSN:1872-7786
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Interaction of double stranded DNA with bulky and hydrophobic Salen type Schiff base complex: [N, N' Bis [3- tert-butyl-5-[triphenyl-phosphonium - methyl] - salicylidene] 1,2 ethylene-diamine nickel(III) acetate (refer to Ni Salen complex) was extensively investigated using the spectroscopic techniques and gel electrophoresis. Absorption titration experiment showed the hypochromic effect and the significant red shift of the complex absorption. In competition experiments with ethidium bromide (EB), Ni Salen complex exhibited non-competitive binding at high concentrations. UV-vis absorption and fluorescence emission data agreed on a binding constant of (1.64 ±â€¯0.01) µM , thereby showing the strong interaction of the complex with DNA; also, a binding site size of 2.33 ±â€¯0.01 base pairs per complex was achieved. Thermal denaturation experiment showed that T of calf thymus-DNA was increased by approximately 10 °C at a molar ratio of the dye/base of 0.2. The CD spectra of DNA exhibited an increase in both positive and negative peaks without any shift in the position of bands upon addition of the complex. The amplitude of the LD spectra of DNA was decreased in the presence of the complex. Reduced linear dichroism (LD ) revealed that the transition moment of complex was parallel to the DNA helix axis. Gel electrophoresis experiments confirmed that Ni Salen complex had no nuclease/DNA cleaving activity; also, DNA-DNA cross links were formed at high concentrations of complex, leading to the aggregation of DNA.
[Mh] Termos MeSH primário: DNA/química
Níquel/química
RNA de Cadeia Dupla/química
Água/química
[Mh] Termos MeSH secundário: Animais
Sítios de Ligação
Bovinos
Eletroforese em Gel de Ágar/métodos
Etídio/química
Etilenodiaminas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ethylenediamines); 0 (RNA, Double-Stranded); 059QF0KO0R (Water); 7OV03QG267 (Nickel); 9007-49-2 (DNA); 91080-16-9 (calf thymus DNA); 94-93-9 (disalicylaldehyde ethylenediamine); EN464416SI (Ethidium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE


  3 / 4551 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29287112
[Au] Autor:Warren EB; Aicher AE; Fessel JP; Konradi C
[Ad] Endereço:Department of Pharmacology, Vanderbilt University, Nashville, Tennessee, United States of America.
[Ti] Título:Mitochondrial DNA depletion by ethidium bromide decreases neuronal mitochondrial creatine kinase: Implications for striatal energy metabolism.
[So] Source:PLoS One;12(12):e0190456, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mitochondrial DNA (mtDNA), the discrete genome which encodes subunits of the mitochondrial respiratory chain, is present at highly variable copy numbers across cell types. Though severe mtDNA depletion dramatically reduces mitochondrial function, the impact of tissue-specific mtDNA reduction remains debated. Previously, our lab identified reduced mtDNA quantity in the putamen of Parkinson's Disease (PD) patients who had developed L-DOPA Induced Dyskinesia (LID), compared to PD patients who had not developed LID and healthy subjects. Here, we present the consequences of mtDNA depletion by ethidium bromide (EtBr) treatment on the bioenergetic function of primary cultured neurons, astrocytes and neuron-enriched cocultures from rat striatum. We report that EtBr inhibition of mtDNA replication and transcription consistently reduces mitochondrial oxygen consumption, and that neurons are significantly more sensitive to EtBr than astrocytes. EtBr also increases glycolytic activity in astrocytes, whereas in neurons it reduces the expression of mitochondrial creatine kinase mRNA and levels of phosphocreatine. Further, we show that mitochondrial creatine kinase mRNA is similarly downregulated in dyskinetic PD patients, compared to both non-dyskinetic PD patients and healthy subjects. Our data support a hypothesis that reduced striatal mtDNA contributes to energetic dysregulation in the dyskinetic striatum by destabilizing the energy buffering system of the phosphocreatine/creatine shuttle.
[Mh] Termos MeSH primário: Corpo Estriado/metabolismo
Creatina Quinase/metabolismo
DNA Mitocondrial/metabolismo
Metabolismo Energético
Etídio/farmacologia
Mitocôndrias/enzimologia
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Glicólise
Seres Humanos
Consumo de Oxigênio
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Nm] Nome de substância:
0 (DNA, Mitochondrial); EC 2.7.3.2 (Creatine Kinase); EN464416SI (Ethidium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171230
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190456


  4 / 4551 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28789597
[Au] Autor:Li X; Grigalavicius M; Li Y; Li X; Zhong Y; Huang R; Yu D; Berge V; Goscinski MA; Kvalheim G; Nesland JM; Suo Z
[Ad] Endereço:1 Department of Pathology, The Norwegian Radium Hospital, Oslo University Hospital and University of Oslo, Oslo, Norway.
[Ti] Título:MtDNA depletion influences the transition of CD44 subtypes in human prostate cancer DU145 cells.
[So] Source:Tumour Biol;39(8):1010428317713671, 2017 Aug.
[Is] ISSN:1423-0380
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Our earlier study revealed that long-term ethidium bromide application causes mitochondrial DNA depletion in human prostate cancer DU145 cell line (DU145 ), and this DU145 subline appears to have expanded CD44 cell population than its parental wild type DU145 cells (DU145 ). Increasing evidence suggests that CD44 cells are highly cancer stem cell like, but it is not clear about their dynamic transition between CD44 and CD44 phenotypes in prostate cancer cells, and how it is affected by mitochondrial DNA depletion. To address these questions, four cell subpopulations were isolated from both DU145 and DU145 cell lines based on their CD44 expression level and mitochondrial membrane potential. The cell motility and colony formation capability of the fluorescence activated cell sorting-sorted cell subpopulations were further examined. It was discovered in the DU145 cells that CD44 cells could transit into both CD44 and CD44 phenotypes and that CD44 cells were prone to sustain their CD44 phenotype as renewal. However, such transition principle was altered in the DU145 cells, in which CD44 cells showed similar capability to sustain a CD44 phenotype, while the transition of CD44 cells to CD44 were suppressed. It is concluded that mitochondrial DNA depletion in the human prostate cancer DU145 cells influences their renewal and CD44 subphenotype transition. Such alterations may be the driving force for the enrichment of CD44 DU145 cells after the mitochondrial DNA depletion, although the molecular mechanisms remain unclear.
[Mh] Termos MeSH primário: Linhagem da Célula/genética
Proliferação Celular/genética
DNA Mitocondrial/genética
Neoplasias da Próstata/genética
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Movimento Celular/genética
Etídio/farmacologia
Citometria de Fluxo
Regulação Neoplásica da Expressão Gênica/genética
Seres Humanos
Receptores de Hialuronatos/biossíntese
Receptores de Hialuronatos/genética
Masculino
Células-Tronco Neoplásicas/efeitos dos fármacos
Neoplasias da Próstata/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CD44 protein, human); 0 (DNA, Mitochondrial); 0 (Hyaluronan Receptors); EN464416SI (Ethidium)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.1177/1010428317713671


  5 / 4551 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
[PMID]:28591344
[Au] Autor:Jordão CP; Fernandes T; Tanaka LY; Bechara LRG; de Sousa LGO; Oliveira EM; Ramires PR
[Ad] Endereço:Unidade de Reabilitação, Instituto do Coracao (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BR.
[Ti] Título:Aerobic Swim Training Restores Aortic Endothelial Function by Decreasing Superoxide Levels in Spontaneously Hypertensive Rats.
[So] Source:Clinics (Sao Paulo);72(5):310-316, 2017 May.
[Is] ISSN:1980-5322
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE:: We aimed to determine whether aerobic training decreases superoxide levels, increases nitric oxide levels, and improves endothelium-dependent vasodilation in the aortas of spontaneously hypertensive rats. METHODS:: Spontaneously hypertensive rats (SHR) and Wistar Kyoto rats (WKY) were distributed into 2 groups: sedentary (SHRsd and WKYsd, n=10 each) and swimming-trained (SHRtr, n=10 and WKYtr, n=10, respectively). The trained group participated in training sessions 5 days/week for 1 h/day with an additional work load of 4% of the animal's body weight. After a 10-week sedentary or aerobic training period, the rats were euthanized. The thoracic aortas were removed to evaluate the vasodilator response to acetylcholine (10-10 to 10-4 M) with or without preincubation with L-NG-nitro-L-arginine methyl ester hydrochloride (L-NAME; 10-4 M) in vitro. The aortic tissue was also used to assess the levels of the endothelial nitric oxide synthase and nicotinamide adenine dinucleotide oxidase subunit isoforms 1 and 4 proteins, as well as the superoxide and nitrite contents. Blood pressure was measured using a computerized tail-cuff system. RESULTS:: Aerobic training significantly increased the acetylcholine-induced maximum vasodilation observed in the SHRtr group compared with the SHRsd group (85.9±4.3 vs. 71.6±5.2%). Additionally, in the SHRtr group, superoxide levels were significantly decreased, nitric oxide bioavailability was improved, and the levels of the nicotinamide adenine dinucleotide oxidase subunit isoform 4 protein were decreased compared to the SHRsd group. Moreover, after training, the blood pressure of the SHRtr group decreased compared to the SHRsd group. Exercise training had no effect on the blood pressure of the WKYtr group. CONCLUSIONS:: In SHR, aerobic swim training decreased vascular superoxide generation by nicotinamide adenine dinucleotide oxidase subunit isoform 4 and increased nitric oxide bioavailability, thereby improving endothelial function.
[Mh] Termos MeSH primário: Aorta Torácica/fisiopatologia
Endotélio Vascular/fisiopatologia
Hipertensão/fisiopatologia
Condicionamento Físico Animal/fisiologia
Superóxidos/análise
Natação/fisiologia
[Mh] Termos MeSH secundário: Animais
Western Blotting
Etídio/análogos & derivados
Teste de Esforço
Fluorescência
Hemodinâmica
Masculino
NAD/análise
NG-Nitroarginina Metil Éster/análise
NG-Nitroarginina Metil Éster/metabolismo
Óxido Nítrico Sintase Tipo III/análise
Óxido Nítrico Sintase Tipo III/metabolismo
Nitritos/análise
Nitritos/metabolismo
Distribuição Aleatória
Ratos Endogâmicos SHR
Valores de Referência
Reprodutibilidade dos Testes
Superóxidos/metabolismo
Fatores de Tempo
Vasodilatação/fisiologia
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nitrites); 0U46U6E8UK (NAD); 104821-25-2 (dihydroethidium); 11062-77-4 (Superoxides); EC 1.14.13.39 (Nitric Oxide Synthase Type III); EN464416SI (Ethidium); V55S2QJN2X (NG-Nitroarginine Methyl Ester)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE


  6 / 4551 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28526754
[Au] Autor:Strassburger K; Lorbeer FK; Lutz M; Graf F; Boutros M; Teleman AA
[Ad] Endereço:German Cancer Research Center (DKFZ), Heidelberg 69120, Germany.
[Ti] Título:Oxygenation and adenosine deaminase support growth and proliferation of cultured wing imaginal discs.
[So] Source:Development;144(13):2529-2538, 2017 07 01.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The wing imaginal disc has been an important model system over past decades for discovering novel biology related to development, signaling and epithelial morphogenesis. Novel experimental approaches have been enabled using a culture setup that allows cultures of wing discs. Current setups, however, are not able to sustain both growth and cell-cycle progression of wing discs We discover here a setup that requires both oxygenation of the tissue and adenosine deaminase activity in the medium, and supports both growth and proliferation of wing discs for 9 h. Nonetheless, further work will be required to extend the duration of the culturing and to enable live imaging of the cultured discs in the future.
[Mh] Termos MeSH primário: Adenosina Desaminase/metabolismo
Drosophila melanogaster/citologia
Drosophila melanogaster/enzimologia
Discos Imaginais/citologia
Oxigênio/metabolismo
Asas de Animais/citologia
[Mh] Termos MeSH secundário: Animais
Proliferação Celular
Células Cultivadas
Ecdisona/metabolismo
Etídio/metabolismo
Corpo Adiposo/citologia
Corpo Adiposo/metabolismo
Insulina/metabolismo
Hormônios Juvenis/metabolismo
Fase S
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insulin); 0 (Juvenile Hormones); 3604-87-3 (Ecdysone); EC 3.5.4.4 (Adenosine Deaminase); EN464416SI (Ethidium); S88TT14065 (Oxygen)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170521
[St] Status:MEDLINE
[do] DOI:10.1242/dev.147538


  7 / 4551 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28272881
[Au] Autor:Brown K; Li W; Kaur P
[Ad] Endereço:Department of Biology, Georgia State University , Atlanta, Georgia 30303, United States.
[Ti] Título:Role of Aromatic and Negatively Charged Residues of DrrB in Multisubstrate Specificity Conferred by the DrrAB System of Streptomyces peucetius.
[So] Source:Biochemistry;56(13):1921-1931, 2017 Apr 04.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Resistance to the anticancer antibiotics, doxorubicin and daunorubicin, in the producer organism Streptomyces peucetius is conferred by an ABC transporter made of two proteins, DrrA and DrrB, which together form a dedicated exporter for these two antibiotics. Surprisingly, however, the DrrAB system exhibits broad substrate specificity overlapping with well-studied multidrug resistance transporters, including P-glycoprotein. Therefore, it provides an excellent model for studying the molecular basis of multispecificity in a prototype efflux system with the potential to unravel the origin and evolution of multidrug resistance. It has been suggested that multispecificity in multidrug exporters may be generally determined by the number and location of aromatic residues. Strategically placed negatively charged residues may also be critical for binding of cationic lipophilic drugs. We selected 13 aromatic and four negatively charged residues on the basis of their location in and/or near the predicted drug-binding pocket of DrrB for analysis. Indeed, mutations of most tested residues drastically inhibited doxorubicin efflux. Interestingly, several mutants lost resistance to doxorubicin and verapamil simultaneously but retained resistance to Hoechst 33342 and/or ethidium bromide, suggesting the presence of overlapping as well as independent drug-binding sites in a common drug-binding pocket of DrrB. This study provides the first comprehensive analysis of residues involved in drug binding in a bacterial multidrug resistance protein of the ABC superfamily, and it shows strong similarity in the molecular mechanism of polyspecific drug recognition between DrrAB and Pgp. Altogether, we conclude that aromatic residue-based multidrug specificity is conserved across domains and over long evolutionary periods. The significance of these findings is discussed.
[Mh] Termos MeSH primário: Transportadores de Cassetes de Ligação de ATP/química
Membro 1 da Subfamília B de Cassetes de Ligação de ATP/química
Proteínas de Bactérias/química
Daunorrubicina/química
Doxorrubicina/química
Verapamil/química
[Mh] Termos MeSH secundário: Transportadores de Cassetes de Ligação de ATP/genética
Transportadores de Cassetes de Ligação de ATP/metabolismo
Membro 1 da Subfamília B de Cassetes de Ligação de ATP/genética
Membro 1 da Subfamília B de Cassetes de Ligação de ATP/metabolismo
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Benzimidazóis/química
Benzimidazóis/metabolismo
Daunorrubicina/metabolismo
Doxorrubicina/metabolismo
Farmacorresistência Bacteriana Múltipla/genética
Escherichia coli/genética
Escherichia coli/metabolismo
Etídio/química
Etídio/metabolismo
Evolução Molecular
Expressão Gênica
Modelos Moleculares
Mutação
Ligação Proteica
Estrutura Secundária de Proteína
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Eletricidade Estática
Streptomyces/genética
Streptomyces/metabolismo
Homologia Estrutural de Proteína
Especificidade por Substrato
Verapamil/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ATP-Binding Cassette, Sub-Family B, Member 1); 0 (Bacterial Proteins); 0 (Benzimidazoles); 0 (DrrB protein, Streptomyces peucetius); 0 (Recombinant Proteins); 80168379AG (Doxorubicin); CJ0O37KU29 (Verapamil); EN464416SI (Ethidium); P976261J69 (bisbenzimide ethoxide trihydrochloride); ZS7284E0ZP (Daunorubicin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170309
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.6b01155


  8 / 4551 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28198513
[Au] Autor:Canela HM; Takami LA; Ferreira ME
[Ad] Endereço:Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
[Ti] Título:SYBR safe efficiently replaces ethidium bromide in Aspergillus fumigatus gene disruption.
[So] Source:Genet Mol Res;16(1), 2017 Feb 08.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Invasive aspergillosis is a disease responsible for high mortality rates, caused mainly by Aspergillus fumigatus. The available drugs are limited and this disease continues to occur at an unacceptable frequency. Gene disruption is essential in the search for new drug targets. An efficient protocol for A. fumigatus gene disruption was described but it requires ethidium bromide, a genotoxic agent, for DNA staining. Therefore, the present study tested SYBR safe , a non-genotoxic DNA stain, in A. fumigatus gene disruption protocol. The chosen gene was cipC, which has already been disrupted successfully in our laboratory. A deletion cassette was constructed in Saccharomyces cerevisiae and used in A. fumigatus transformation. There was no statistical difference between the tested DNA stains. The success rate of S. cerevisiae transformation was 63.3% for ethidium bromide and 70% for SYBR safe . For A. fumigatus gene disruption, the success rate for ethidium bromide was 100 and 97% for SYBR safe . In conclusion, SYBR safe efficiently replaced ethidium bromide, making this dye a safe and efficient alternative for DNA staining in A. fumigatus gene disruption.
[Mh] Termos MeSH primário: Aspergillus fumigatus/efeitos dos fármacos
Aspergillus fumigatus/genética
Dano ao DNA/efeitos dos fármacos
Etídio/toxicidade
Corantes Fluorescentes/toxicidade
[Mh] Termos MeSH secundário: Saccharomyces cerevisiae/efeitos dos fármacos
Saccharomyces cerevisiae/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fluorescent Dyes); EN464416SI (Ethidium)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170316
[Lr] Data última revisão:
170316
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170216
[St] Status:MEDLINE
[do] DOI:10.4238/gmr16019583


  9 / 4551 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28183660
[Au] Autor:Ramachandran R; Krishnaraj C; Sivakumar AS; Prasannakumar P; Abhay Kumar VK; Shim KS; Song CG; Yun SI
[Ad] Endereço:Centre for Advanced Studies in Botany, School of Life Sciences, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India.
[Ti] Título:Anticancer activity of biologically synthesized silver and gold nanoparticles on mouse myoblast cancer cells and their toxicity against embryonic zebrafish.
[So] Source:Mater Sci Eng C Mater Biol Appl;73:674-683, 2017 Apr 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to evaluate the anticancer activity of bioinspired silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) against mouse myoblast cancer cells (C C ). Both AgNPs and AuNPs were biologically synthesized using Spinacia oleracea Linn., aqueous leaves extract. UV-Vis. spectrophotometer, high resolution-transmission electron microscopy (HR-TEM), field emission-scanning electron microscopy (FE-SEM) and X-ray diffraction (XRD) studies supported the successful synthesis of AgNPs and AuNPs. Both these NPs have shown cytotoxicity against C C cells even at very low concentration (5µg/mL). Acridine orange/Ethidium bromide (AO/EB) dual staining confirmed the apoptotic morphological features. The levels of caspase enzymes (caspase-3 and caspase-7) were significantly up-regulated in NPs treated myoblast cells than the plant extract. Furthermore, in zebrafish embryo toxicity study, AgNPs showed 100% mortality at 3µg/mL concentration while AuNPs exhibited the same at much higher concentration (300mg/mL). Taken together, these results provide a preliminary guidance for the development of biomaterials based drugs to fight against the fatal diseases for example cancer.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Embrião não Mamífero/efeitos dos fármacos
Ouro/farmacologia
Nanopartículas Metálicas/toxicidade
Mioblastos/patologia
Prata/farmacologia
Testes de Toxicidade
Peixe-Zebra/embriologia
[Mh] Termos MeSH secundário: Laranja Acridina/metabolismo
Animais
Apoptose/efeitos dos fármacos
Caspases/metabolismo
Linhagem Celular Tumoral
Forma Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Embrião não Mamífero/anormalidades
Etídio/metabolismo
Nanopartículas Metálicas/ultraestrutura
Camundongos
Mioblastos/efeitos dos fármacos
Técnicas Fotoacústicas
Extratos Vegetais/farmacologia
Folhas de Planta/química
Spinacia oleracea/química
Coloração e Rotulagem
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Plant Extracts); 3M4G523W1G (Silver); 7440-57-5 (Gold); EC 3.4.22.- (Caspases); EN464416SI (Ethidium); F30N4O6XVV (Acridine Orange)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170903
[Lr] Data última revisão:
170903
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE


  10 / 4551 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28117677
[Au] Autor:Wang X; Yan M; Wang Q; Wang H; Wang Z; Zhao J; Li J; Zhang Z
[Ad] Endereço:School of Pharmacy, Jining Medical University, 669 Xueyuan Road, Rizhao 276800, Shandong, China. wxcwayne@126.com.
[Ti] Título:In Vitro DNA-Binding, Anti-Oxidant and Anticancer Activity of Indole-2-Carboxylic Acid Dinuclear Copper(II) Complexes.
[So] Source:Molecules;22(1), 2017 Jan 20.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Indole-2-carboxylic acid copper complex (ICA-Cu) was successfully prepared and characterized through elemental analysis, IR, UV-Vis, ¹H-NMR, TG analysis, and molar conductance, and its molecular formula was [Cu2(C9H6O2N)4(H2O)2]·2H2O. The binding ability of ICA-Cu to calf thymus DNA (CT-DNA) was examined by fluorescence spectrometry and the viscosity method. The results indicated that, upon the addition of increasing amounts of CT-DNA, the excitation and emission intensity of ICA-Cu decreased obviously and the excitation spectra shifted towards a long wavelength. ICA-Cu could displace ethidium bromide (EB) from the EB-DNA system, making the fluorescence intensity of the EB-DNA system decrease sharply; the quenching constant value was 3.99 × 104 M . The emission intensity of the ICA-Cu-DNA system was nearly constant, along with the addition of Na⁺ in a series of concentrations. The fluorescence of the complex could be protected after the complex interacted with DNA. A viscosity measurement further supported the result that the ICA-Cu complex may interact with DNA in an intercalative binding mode. The antioxidant activities of ICA-Cu were evaluated by a 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, a hydroxyl radical (OH) scavenging assay, and a 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay. The ICA-Cu exhibited the highest inhibitory effects on the ABTS radical (94% inhibition at 60 µM), followed by OH and DPPH radicals (the degrees of inhibition being 71% and 56%, respectively). The in vitro cytotoxicity activity of ICA-Cu against two human breast cancer cell lines, MDA-MB-231 and MCF-7, was investigated by 3-[4,5-dimethyltiazol2-yl]-2.5-diphenyl-tetrazolium bromide (MTT) assay and cellular morphological analysis. The results showed that, upon increasing the concentration of ICA-Cu, an increase was observed in growth-inhibitory activity and the inhibition percentage were greater than 90% at 20 µM in both cell lines. Also, cellular morphological changes in the two cell lines agreed with the cytotoxicity results.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Neoplasias da Mama/tratamento farmacológico
Cobre/química
Indóis/química
Indóis/farmacologia
[Mh] Termos MeSH secundário: Animais
Antioxidantes/química
Bovinos
Linhagem Celular Tumoral
DNA/metabolismo
Etídio/metabolismo
Seres Humanos
Células MCF-7
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Indoles); 1477-50-5 (indole-2-carboxylic acid); 789U1901C5 (Copper); 9007-49-2 (DNA); 91080-16-9 (calf thymus DNA); EN464416SI (Ethidium)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170523
[Lr] Data última revisão:
170523
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170125
[St] Status:MEDLINE



página 1 de 456 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde