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[PMID]:29336193
[Au] Autor:Prandina A; Herfindal L; Radix S; Rongved P; Døskeland SO; Le Borgne M; Perret F
[Ad] Endereço:a Université de Lyon, Université Claude Bernard Lyon 1, Faculté de Pharmacie - ISPB, EA 4446 Bioactive Molecules and Medicinal Chemistry, SFR Santé Lyon-Est CNRS UMS3453 - INSERM US7 , Lyon Cedex , France.
[Ti] Título:Enhancement of iodinin solubility by encapsulation into cyclodextrin nanoparticles.
[So] Source:J Enzyme Inhib Med Chem;33(1):370-375, 2018 Dec.
[Is] ISSN:1475-6374
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phenazine is known to regroup planar nitrogen-containing heterocyclic compounds. It was used here to enhance the bioavailability of the biologically important compound iodinin, which is near insoluble in aqueous solutions. Its water solubility has led to the development of new formulations using diverse amphiphilic α-cyclodextrins (CDs). With the per-[6-desoxy-6-(3-perfluorohexylpropanethio)-2,3-di-O-methyl]-α-CD, we succeeded to get iodinin-loaded nanoformulations with good parameters such as a size of 97.9 nm, 62% encapsulation efficiency and efficient control release. The study presents an interesting alternative to optimizing the water solubility of iodinin by chemical modifications of iodinin.
[Mh] Termos MeSH primário: Nanopartículas/química
Fenazinas/química
alfa-Ciclodextrinas/química
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Linhagem Celular Tumoral
Relação Dose-Resposta a Droga
Estrutura Molecular
Tamanho da Partícula
Fenazinas/farmacologia
Ratos
Solubilidade
Relação Estrutura-Atividade
Propriedades de Superfície
alfa-Ciclodextrinas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phenazines); 0 (alpha-Cyclodextrins); 0 (phenazine); G1239096EO (iodinin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE
[do] DOI:10.1080/14756366.2017.1421638


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[PMID]:29227933
[Au] Autor:Krishnaiah M; de Almeida NR; Udumula V; Song Z; Chhonker YS; Abdelmoaty MM; do Nascimento VA; Murry DJ; Conda-Sheridan M
[Ad] Endereço:Department of Pharmaceutical Sciences, College of Pharmacy, University of Nebraska Medical Center, Omaha, NE 68198, USA.
[Ti] Título:Synthesis, biological evaluation, and metabolic stability of phenazine derivatives as antibacterial agents.
[So] Source:Eur J Med Chem;143:936-947, 2018 Jan 01.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Drug-resistant pathogens are a major cause of hospital- and community-associated bacterial infections in the United States and around the world. These infections are increasingly difficult to treat due to the development of antibiotic resistance and the formation of bacterial biofilms. In the paper, a series of phenazines were synthesized and evaluated for their in vitro antimicrobial activity against Gram positive (methicillin resistant staphylococcus aureus, MRSA) and Gram negative (Escherichia coli, E. coli) bacteria. The compound 6,9-dichloro-N-(methylsulfonyl)phenazine-1-carboxamide (18c) proved to be the most active molecule (MIC = 16 µg/mL) against MRSA whereas 9-methyl-N-(methylsulfonyl)phenazine-1-carboxamide (30e) showed good activity against both MRSA (MIC = 32 µg/mL) and E. coli (MIC = 32 µg/mL). Molecule 18c also demonstrated significant biofilm dispersion and inhibition against S. aureus. Preliminary studies indicate the molecules do not disturb bacterial membranes and there activity is not directly linked to the generation of reactive oxygen species. Compound 18c displayed minor toxicity against mammalian cells. Metabolic stability studies of the most promising compounds indicate stability towards phase I and phase II metabolizing enzymes.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Positivas/efeitos dos fármacos
Fenazinas/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/química
Antibacterianos/metabolismo
Biofilmes/efeitos dos fármacos
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Seres Humanos
Testes de Sensibilidade Microbiana
Estrutura Molecular
Fenazinas/química
Fenazinas/metabolismo
Teoria Quântica
Espécies Reativas de Oxigênio/metabolismo
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Phenazines); 0 (Reactive Oxygen Species); 0 (phenazine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180101
[Lr] Data última revisão:
180101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE


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[PMID]:28802172
[Au] Autor:Sen S; Sett R; Paul BK; Guchhait N
[Ad] Endereço:Department of Chemistry, University of Calcutta, Kolkata 700 009, India.
[Ti] Título:Interaction of phenazinium-based photosensitizers with the 'N' and 'B' isoforms of human serum albumin: Effect of methyl substitution.
[So] Source:J Photochem Photobiol B;174:217-228, 2017 Sep.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The present work is focused on exploring the interaction of two phenazinium-based biological photosensitizers, phenosafranin (PSF) and safranin-O (SO), with human serum albumin (HSA), with particular emphasis on the physiologically significant NB conformational transition of the protein on the dye:HSA interaction. In addition, the presence of methyl substitution on the planar phenazinium ring in SO paves way for looking into the effect of simple chemical manipulation (that is, methyl substitution on the dye nucleus) on the dye:protein interaction behavior as a function of various (pH-induced) isoforms of HSA. Our results reveal a significantly stronger binding interaction of SO with the B isoform of HSA (at pH9.0) compared to that with the N isoform (at pH7.4). On the contrary, the PSF:HSA interaction is found to be reasonably insensitive to the aforesaid conformational transition of HSA. However, the probable binding location of both the dye molecules (PSF and SO) is found to be within the protein scaffolds (domain IB). This is further quantified from the modulation of fluorescence decay behavior of the dyes within the protein scaffolds. It is important to note that the rotational relaxation behavior of the protein-bound dyes reveals an unusual 'dip-rise-dip', an observation not reported earlier. Such unusual anisotropy decay is meticulously analyzed by an associated (or multicomponent) exponential decay model which emphasizes on the fractional contributions from differential classes of fluorophore populations characterized by the fast (due to unbound or solvent exposed part of the fluorophore) and slow (due to embedded or bound part) motions, in combination with their different local mobilities. Furthermore, the translational diffusion of the dye molecules in the presence of the protein in different isoforms (N-form or B-form) at a single molecule level is also measured by Fluorescence Correlation Spectroscopy (FCS).
[Mh] Termos MeSH primário: Fenazinas/química
Fenazinas/metabolismo
Fármacos Fotossensibilizantes/química
Fármacos Fotossensibilizantes/metabolismo
Albumina Sérica/metabolismo
[Mh] Termos MeSH secundário: Corantes Fluorescentes/química
Corantes Fluorescentes/metabolismo
Seres Humanos
Modelos Moleculares
Conformação Molecular
Ligação Proteica
Isoformas de Proteínas/metabolismo
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fluorescent Dyes); 0 (Phenazines); 0 (Photosensitizing Agents); 0 (Protein Isoforms); 0 (Serum Albumin); 81-93-6 (phenosafranine); XTX0YXU2HV (safranine T)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170813
[St] Status:MEDLINE


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[PMID]:28625706
[Au] Autor:Neeraja M; Lakshmi V; Padmasri C; Padmaja K
[Ad] Endereço:Dept. of Microbiology, Nizam's Institute of Medical Sciences, Hyderabad, Telangana, India.
[Ti] Título:Utility of Acridine Orange staining for detection of bacteria from positive blood cultures.
[So] Source:J Microbiol Methods;139:215-217, 2017 Aug.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The diagnostic performance of AO stain was evaluated for the detection of bacteria and or fungi from positive blood cultures. The sensitivity of Gram stain (GS) was 98.26% while Acridine Orange (AO) stain proved to be more sensitive (100%) with a Positive and Negative Predictive Value of 100% each. The specificity of both the stains was 100%. Overall agreement between the two stains was 98.23% (688/700). The organisms that were missed by GS and positive by AO were Candida species (Sutton, 2006) and Gram negative bacilli (GNB) (Sutton, 2006). Sensitivity of GS was 82.35% and AO was 100% among mixed cultures. Immediate reporting of the results of AO stain would have a significant impact on clinical management of patients with serious blood stream infections.
[Mh] Termos MeSH primário: Laranja Acridina
Bacteriemia/microbiologia
Bactérias/isolamento & purificação
Hemocultura
Corantes
[Mh] Termos MeSH secundário: Bacteriemia/diagnóstico
Hemocultura/normas
Corantes/química
Violeta de Genciana
Bactérias Gram-Negativas/isolamento & purificação
Bactérias Gram-Negativas/metabolismo
Seres Humanos
Fenazinas
Sensibilidade e Especificidade
Coloração e Rotulagem/economia
Leveduras/isolamento & purificação
Leveduras/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coloring Agents); 0 (Gram's stain); 0 (Phenazines); F30N4O6XVV (Acridine Orange); J4Z741D6O5 (Gentian Violet)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE


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[PMID]:28575838
[Au] Autor:Qiao YJ; Qiao Y; Zou L; Wu XS; Liu JH
[Ad] Endereço:Faculty of Materials & Energy, Chongqing Key Laboratory for Advanced Materials & Technologies of Clean Energies, Southwest University, Chongqing 400715, China.
[Ti] Título:Biofilm promoted current generation of Pseudomonas aeruginosa microbial fuel cell via improving the interfacial redox reaction of phenazines.
[So] Source:Bioelectrochemistry;117:34-39, 2017 Oct.
[Is] ISSN:1878-562X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Bacteria biofilm plays a key role in current generation of microbial fuel cells (MFCs), especially for the start-up stage. However, the detailed mechanism of the biofilm promoting the power generation is not very clear so far, especially for those exoelectrogens who rely on the self-excreted electron mediators for extracellular electron transfer. In this work, a biofilm formation inhibitor-sodium houttuyfonate (SH) is used to build a "non-biofilm" anode of Pseudomonas aeruginosa (P. aeruginosa) without affecting the bacteria growth during the MFC operation. According to the comparison results of the "non-biofilm" anode and biofilm-covered anode on current generation, phenazines concentration variation and anodic electrocatalysis, the biofilm on the anode not only provides plenty of bacterial cells for catalysis but also promotes the interfacial phenazine redox reaction through accumulating the self-generated mediators on anode for fast interfacial electron transfer. This work proves that the biofilm assisted electron mediator accumulation will benefit such kind of exoelectrogens to sustain sufficient electron mediators for extracellular electron transfer.
[Mh] Termos MeSH primário: Fontes de Energia Bioelétrica/microbiologia
Biofilmes
Condutividade Elétrica
Fenazinas/metabolismo
Pseudomonas aeruginosa/fisiologia
[Mh] Termos MeSH secundário: Eletroquímica
Eletrodos
Transporte de Elétrons
Pseudomonas aeruginosa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phenazines)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170815
[Lr] Data última revisão:
170815
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170603
[St] Status:MEDLINE


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[PMID]:28549584
[Au] Autor:Lan T; Zhao H; Xiang B; Wang J; Liu Y
[Ad] Endereço:Department of Orthodontics, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
[Ti] Título:Suture compression induced midpalatal suture chondrocyte apoptosis with increased caspase-3, caspase-9, Bad, Bak, Bax and Bid expression.
[So] Source:Biochem Biophys Res Commun;489(2):179-186, 2017 Jul 22.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Previous studies found bone resorption and chondrocytes loss in mouse models of mid-palatal suture when given continuous compressive force, although chondrocytes response remained unknown. Herein, we design this study to determine how continuous compression force induces chondrocytes apoptosis. METHODS: Thirty C57BL/6 male mice (aged 6 weeks) were randomly assigned into controls (not ligated to a spring), blank controls (ligated with no compression) and the compression group (ligated with 20-g compression). After 4 d, palatal tissues were sampled and stained by TB and safranin-O. Tunel staining measured the percentage of apoptotic chondrocytes, and immunohistochemistry was performed to label apoptosis-associated proteins (e.g., Bcl-2, Bcl-xl, Bax, Bak, Bid, Bad, caspase-3, caspase-8 and caspase-9). Intergroup comparison was made by the rank sum test, and P < 0.05 was defined as statistical significance. RESULTS: After 7d of induction, TB and safranin-O staining revealed that the cartilage area in the compression group was significantly decreased, while the control group remained largely unaltered. Tunel staining showed that apoptotic cell numbers in the mid-palatal suture were significantly higher than the control group. Immunohistochemistry showed that mice in the compression group had significantly increased expression of caspase-3, caspase-9, Bad, Bak, Bax and Bid; However, caspase-8 remained unaltered. No expression of Bcl-2 and Bcl-xl was detected. CONCLUSIONS: Continuous compression force induces chondrocytes apoptosis in the mid-palatal suture. This process might be associated with the mitochondrial pathway.
[Mh] Termos MeSH primário: Apoptose
Condrócitos/metabolismo
Condrócitos/patologia
Pressão/efeitos adversos
Suturas/efeitos adversos
Regulação para Cima
[Mh] Termos MeSH secundário: Animais
Proteína Agonista de Morte Celular de Domínio Interatuante com BH3/biossíntese
Caspase 3/biossíntese
Caspase 9/biossíntese
Imuno-Histoquímica
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Fenazinas
Cloreto de Tolônio
Proteína Killer-Antagonista Homóloga a bcl-2/biossíntese
Proteína X Associada a bcl-2/biossíntese
Proteína de Morte Celular Associada a bcl/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (BH3 Interacting Domain Death Agonist Protein); 0 (Bad protein, mouse); 0 (Bak1 protein, mouse); 0 (Bax protein, mouse); 0 (Bid protein, mouse); 0 (Phenazines); 0 (bcl-2 Homologous Antagonist-Killer Protein); 0 (bcl-2-Associated X Protein); 0 (bcl-Associated Death Protein); 15XUH0X66N (Tolonium Chloride); EC 3.4.22.- (Casp3 protein, mouse); EC 3.4.22.- (Casp9 protein, mouse); EC 3.4.22.- (Caspase 3); EC 3.4.22.- (Caspase 9); XTX0YXU2HV (safranine T)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170528
[St] Status:MEDLINE


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[PMID]:28494227
[Au] Autor:Berger C; Rosenbaum MA
[Ad] Endereço:Institute of Applied Microbiology - iAMB, Aachen Biology and Biotechnology - ABBt, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany. Electronic address: carola.berger@rwth-aachen.de.
[Ti] Título:Spontaneous quorum sensing mutation modulates electroactivity of Pseudomonas aeruginosa PA14.
[So] Source:Bioelectrochemistry;117:1-8, 2017 Oct.
[Is] ISSN:1878-562X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Pseudomonas aeruginosa is able to interact with the anode of a bioelectrochemical system through redox active phenazines. Earlier studies showed that this interaction is strain and carbon source dependent. With a spontaneously formed ΔlasR mutant of P. aeruginosa PA14 and the wildtype, we investigated the connection between the complex quorum sensing network and current production. Depending on the carbon source, phenazine production and subsequently current generation are effected differently in these two populations. In glucose-fed cultures, the lack of the LasR regulator led to a shift in phenazine concentration, relative composition, and time profiles. In contrast, with the common fermentation product 2,3-butanediol as carbon substrate, no phenazine production was detected for the ΔlasR mutant. For the wildtype, this carbon source is known to induce phenazine synthesis and elevated current production. This work supports the earlier hypothesis of a signaling link between 2,3-butanediol and the quorum-sensing regulatory system and extends this hypothesis to predict a lasR-dependent interaction. The wildtype and mutant population were also evaluated in direct competition, showing strong initial dominance of the wildtype but a higher survival rate of the ΔlasR mutant in later stages of growth. We found no evidence for strong social interactions between these two subpopulations.
[Mh] Termos MeSH primário: Condutividade Elétrica
Mutação
Pseudomonas aeruginosa/citologia
Pseudomonas aeruginosa/genética
Percepção de Quorum/genética
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Eletroquímica
Fenazinas/metabolismo
Pseudomonas aeruginosa/metabolismo
Transativadores/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (LasR protein, Pseudomonas aeruginosa); 0 (Phenazines); 0 (Trans-Activators); 0 (phenazine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170815
[Lr] Data última revisão:
170815
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170512
[St] Status:MEDLINE


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[PMID]:28482217
[Au] Autor:Kumar P; Dasari S; Patra AK
[Ad] Endereço:Department of Chemistry, Indian Institute of Technology Kanpur, Kanpur 208016, Uttar Pradesh, India.
[Ti] Título:Ruthenium(II) complexes of saccharin with dipyridoquinoxaline and dipyridophenazine: Structures, biological interactions and photoinduced DNA damage activity.
[So] Source:Eur J Med Chem;136:52-62, 2017 Aug 18.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Ruthenium complexes trans-[Ru(sac) (dpq) ] (1) and trans-[Ru(sac) (dppz) ] (2) where sac is artificial sweetener saccharin (o-sulfobenzimide; 1,2-benzothiazole-3(2H)-one1,1-dioxide (Hsac)), dpq = dipyrido[3,2-d:2',3'-f]quinoxaline and dppz = dipyrido[3,2-a:2',3'-c]phenazine have been synthesized and thoroughly characterized using various analytical and spectral techniques. Saccharin known to act as carbonic anhydrase IX (CA IX) inhibitor which is a biomarker for highly aggressive and proliferative tumor in hypoxic stress, so inhibition of CA IX is a potential strategy for anticancer chemotherapy. The solid state structures, photophysical properties, photostability, DNA and protein binding affinity, and DNA photocleavage activity were explored. The structural analysis revealed Ru(II) centre is in discrete mononuclear, distorted octahedral {RuN } coordination geometry with two monoanionic nitrogen donor saccharinate ligands and two neutral bidentate nitrogen donors ligands dpq and dppz. cis-[Ru(sac) (dppz) ] (cis-2) geometrical isomer was also isolated and structurally characterized by X-ray crystallography. The photo-induced dissociation of monodentate saccharin ligand is observed when irradiated at UV-A light of 365 nm. The complexes show significant binding affinity to the calf thymus DNA (K âˆ¼ 10 M ) through significant intercalation through planar dpq and dppz ligands. Interaction of complexes 1 and 2 with bovine serum albumin (BSA) showed remarkable tryptophan emission quenching (K ∼10 M ). The complexes showed appreciable photoinduced DNA cleavage activity upon irradiation of low power UV-A light of 365 nm from supercoiled (SC) to its nicked circular (NC) form at micromolar complex concentrations. Photocleavage mechanistic studies in presence of O reveals involvement of reactive oxygen species (ROS) mediated through ligand-centered ππ* and/or MLCT excited states generated upon photoactivation leads to nicking of supercoiled DNA to nicked circular form. In absence of O , we also observed photocleavage of DNA through formation of photoinduced ligand dissociated Ru-DNA complex involving PACT pathway.
[Mh] Termos MeSH primário: DNA/efeitos dos fármacos
Compostos Organometálicos/farmacologia
Fenazinas/farmacologia
Quinoxalinas/farmacologia
Rutênio/farmacologia
Sacarina/farmacologia
[Mh] Termos MeSH secundário: Animais
Bovinos
Dano ao DNA
Relação Dose-Resposta a Droga
Estrutura Molecular
Compostos Organometálicos/síntese química
Compostos Organometálicos/química
Fenazinas/química
Processos Fotoquímicos
Quinoxalinas/química
Rutênio/química
Sacarina/química
Relação Estrutura-Atividade
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Organometallic Compounds); 0 (Phenazines); 0 (Quinoxalines); 7UI0TKC3U5 (Ruthenium); 9007-49-2 (DNA); 91080-16-9 (calf thymus DNA); FST467XS7D (Saccharin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170509
[St] Status:MEDLINE


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[PMID]:28383281
[Au] Autor:Jaaffar AKM; Parejko JA; Paulitz TC; Weller DM; Thomashow LS
[Ad] Endereço:First and second authors: Department of Plant Pathology, Washington State University, Pullman 99164-6430; and third, fourth, and fifth authors: United States Department of Agriculture-Agricultural Research Service, Wheat Health, Genetics and Quality Research Unit, Pullman, WA 99164-6430.
[Ti] Título:Sensitivity of Rhizoctonia Isolates to Phenazine-1-Carboxylic Acid and Biological Control by Phenazine-Producing Pseudomonas spp.
[So] Source:Phytopathology;107(6):692-703, 2017 06.
[Is] ISSN:0031-949X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Rhizoctonia solani anastomosis groups (AG)-8 and AG-2-1 and R. oryzae are ubiquitous in cereal-based cropping systems of the Columbia Plateau of the Inland Pacific Northwest and commonly infect wheat. AG-8 and R. oryzae, causal agents of Rhizoctonia root rot and bare patch, are most commonly found in fields in the low-precipitation zone, whereas R. solani AG-2-1 is much less virulent on wheat and is distributed in fields throughout the low-, intermediate-, and high-precipitation zones. Fluorescent Pseudomonas spp. that produce the antibiotic phenazine-1-carboxylic acid (PCA) also are abundant in the rhizosphere of crops grown in the low-precipitation zone but their broader geographic distribution and effect on populations of Rhizoctonia is unknown. To address these questions, we surveyed the distribution of PCA producers (Phz ) in 59 fields in cereal-based cropping systems throughout the Columbia Plateau. Phz Pseudomonas spp. were detected in 37 of 59 samples and comprised from 0 to 12.5% of the total culturable heterotrophic aerobic rhizosphere bacteria. The frequency with which individual plants were colonized by Phz pseudomonads ranged from 0 to 100%. High and moderate colonization frequencies of Phz pseudomonads were associated with roots from fields located in the driest areas whereas only moderate and low colonization frequencies were associated with crops where higher annual precipitation occurs. Thus, the geographic distribution of Phz pseudomonads overlaps closely with the distribution of R. solani AG-8 but not with that of R. oryzae or R. solani AG-2-1. Moreover, linear regression analysis demonstrated a highly significant inverse relationship between annual precipitation and the frequency of rhizospheres colonized by Phz pseudomonads. Phz pseudomonads representative of the four major indigenous species (P. aridus, P. cerealis, P. orientalis, and P. synxantha) suppressed Rhizoctonia root rot of wheat when applied as seed treatments. In vitro, mean 50% effective dose values for isolates of AG-8 and AG-2-1 from fields with high and low frequencies of phenazine producers did not differ significantly, nor was there a correlation between virulence of an isolate and sensitivity to PCA, resulting in rejection of the hypothesis that tolerance in Rhizoctonia spp. to PCA develops in nature upon exposure to Phz pseudomonads.
[Mh] Termos MeSH primário: Hordeum/microbiologia
Doenças das Plantas/prevenção & controle
Pseudomonas/química
Rhizoctonia/efeitos dos fármacos
[Mh] Termos MeSH secundário: Agentes de Controle Biológico
Produtos Agrícolas
Grãos Comestíveis/microbiologia
Geografia
Concentração de Íons de Hidrogênio
Fenazinas/metabolismo
Fenazinas/farmacologia
Doenças das Plantas/microbiologia
Raízes de Plantas/microbiologia
Pseudomonas/fisiologia
Rhizoctonia/crescimento & desenvolvimento
Rizosfera
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Biological Control Agents); 0 (Phenazines); 2538-68-3 (1-phenazinecarboxylic acid)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170720
[Lr] Data última revisão:
170720
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE
[do] DOI:10.1094/PHYTO-07-16-0257-R


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[PMID]:28351621
[Au] Autor:Tochigi Y; Iwasaki Y; Sano M; Yasuda H; Katayama K; Suzuki H
[Ad] Endereço:Laboratory of Veterinary Physiology, Department of Basic Veterinary Medicine, Division of Functional Morphology, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan.
[Ti] Título:Critical roles of Astrin in the mitosis of immature rat Sertoli cells.
[So] Source:Biochem Biophys Res Commun;486(4):958-964, 2017 May 13.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Male hypogonadism (hgn/hgn) rats show testicular hypoplasia accompanied by dysplastic development of seminiferous tubules due to loss-of-function mutation of the gene encoding Astrin, which is required for mitotic progression in the division cycle of HeLa cells. In the present study, we examined the cytological base leading to the decrease of Sertoli cells in hgn/hgn testes. In hgn/hgn testes on postnatal day 3, anti-phospho-histone H3 (Ser10) (pH3)-positive mitotic phase and TUNEL-positive apoptosis increased in GATA4-positive Sertoli cells. Isolated immature Sertoli cells from hgn/hgn testes showed increased pH3-assessed mitotic index accompanied by decreased 5-bromo-2'-deoxyuridine-incorporation and increased TUNEL-positive apoptosis, suggesting mitotic delay and cell death. In the visualization of mitotic progression by nocodazole (NOC)-mediated cell cycle arrest and subsequent release, hgn/hgn rat-derived Sertoli cells failed to make the transition from prometaphase to metaphase, and the cells with micronuclei and TUNEL-positive cells gradually increased in a time-dependent manner. Western blot analysis detected ≈142 kDa protein expected as Astrin in extracts of +/+ and +/hgn testes and cultured normal Sertoli cells but not in extracts of hgn/hgn testes. CLASP1 was detected in extracts of both normal and hgn/hgn testes, whereas it was localized in kinetochore of normal mitotic Sertoli cells but diffused in cytoplasm of hgn/hgn Sertoli cells. These results indicate that Astrin is required for normal mitotic progression in immature Sertoli cells and that the most severe type of testicullar dysplasia in hgn/hgn rats is caused by mitotic cell death of immature Sertoli cells due to lack of Astrin.
[Mh] Termos MeSH primário: Azul Alciano/metabolismo
Apoptose/fisiologia
Cinetocoros/metabolismo
Proteínas Associadas aos Microtúbulos/metabolismo
Mitose/fisiologia
Fenazinas/metabolismo
Fenotiazinas/metabolismo
Resorcinóis/metabolismo
Células de Sertoli/fisiologia
[Mh] Termos MeSH secundário: Animais
Núcleo Celular/metabolismo
Células Cultivadas
Masculino
Camundongos Endogâmicos
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CLASP1 protein, rat); 0 (Microtubule-Associated Proteins); 0 (Phenazines); 0 (Phenothiazines); 0 (Resorcinols); 83097-09-0 (astrin); P4448TJR7J (Alcian Blue)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170619
[Lr] Data última revisão:
170619
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170330
[St] Status:MEDLINE



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