[PMID]: | 29484749 |
[Au] Autor: | Khosravi AD; Meghdadi H; Ghadiri AA; Alami A; Sina AH; Mirsaeidi M |
[Ad] Endereço: | Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. |
[Ti] Título: | rpoB gene mutations among Mycobacterium tuberculosis isolates from extrapulmonary sites. |
[So] Source: | APMIS;126(3):241-247, 2018 Mar. |
[Is] ISSN: | 1600-0463 |
[Cp] País de publicação: | Denmark |
[La] Idioma: | eng |
[Ab] Resumo: | The aim of this study was to analyze mutations occurring in the rpoB gene of Mycobacterium tuberculosis (MTB) isolates from clinical samples of extrapulmonary tuberculosis (EPTB). Seventy formalin-fixed, paraffin-embedded samples and fresh tissue samples from confirmed EPTB cases were analyzed. Nested PCR based on the rpoB gene was performed on the extracted DNAs, combined with cloning and subsequent sequencing. Sixty-seven (95.7%) samples were positive for nester PCR. Sequence analysis of the 81 bp region of the rpoB gene demonstrated mutations in 41 (61.2%) of 67 sequenced samples. Several point mutations including deletion mutations at codons 510, 512, 513 and 515, with 45% and 51% of the mutations in codons 512 and 513 respectively were seen, along with 26% replacement mutations at codons 509, 513, 514, 518, 520, 524 and 531. The most common alteration was Gln → His, at codon 513, presented in 30 (75.6%) isolates. This study demonstrated sequence alterations in codon 513 of the 81 bp region of the rpoB gene as the most common mutation occurred in 75.6% of molecularly confirmed rifampin-resistant strains. In addition, simultaneous mutation at codons 512 and 513 was demonstrated in 34.3% of the isolates. |
[Mh] Termos MeSH primário: |
Antibióticos Antituberculose/farmacologia Proteínas de Bactérias/genética RNA Polimerases Dirigidas por DNA/genética Farmacorresistência Bacteriana/genética Mycobacterium tuberculosis/genética Rifampina/farmacologia Tuberculose/microbiologia
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[Mh] Termos MeSH secundário: |
Adulto Idoso Idoso de 80 Anos ou mais Células Cultivadas Feminino Seres Humanos Masculino Testes de Sensibilidade Microbiana Meia-Idade Mycobacterium tuberculosis/efeitos dos fármacos Mycobacterium tuberculosis/isolamento & purificação Mutação Puntual/genética Deleção de Sequência/genética Adulto Jovem
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Antibiotics, Antitubercular); 0 (Bacterial Proteins); 0 (rpoB protein, Mycobacterium tuberculosis); EC 2.7.7.6 (DNA-Directed RNA Polymerases); VJT6J7R4TR (Rifampin) |
[Em] Mês de entrada: | 1803 |
[Cu] Atualização por classe: | 180309 |
[Lr] Data última revisão:
| 180309 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 180228 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1111/apm.12804 |
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