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[PMID]: | 28689421 |
[Au] Autor: | Li X |
[Ad] Endereço: | School of Chinese Herbal Medicine and Innovative Research and Development Laboratory of TCM, Guangzhou University of Chinese Medicine , 232 Waihuan East Road, Guangzhou Higher Education Mega Center, Panyu District, Guangzhou, Guangdong 510006, People's Republic of China. |
[Ti] Título: | 2-Phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-Oxide (PTIO ) Radical Scavenging: A New and Simple Antioxidant Assay In Vitro. |
[So] Source: | J Agric Food Chem;65(30):6288-6297, 2017 Aug 02. | [Is] ISSN: | 1520-5118 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | Current in vitro antioxidant assays have several limitations, which frequently cause inconsistent results. The study develops a new antioxidant assay using the 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide radical (PTIO ). After the investigation of various factors, the experimental protocol was briefly recommended as follows: PTIO and the sample solution were added to phosphate buffer (pH 7.4, 50 mM), incubated at 37 °C for 2 h, and then spectrophotometrically measured at 557 nm. The validation test based on 20 pure compounds and 30 lyophilized aqueous extracts suggested that PTIO scavenging had a good linear relationship, stability, and reproducibility. In the ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry analysis, PTIO was observed to give m/z 234 when encountering l-ascorbic acid. As an antioxidant assay, PTIO scavenging possesses four advantages, i.e., oxygen-centered radical, physiological aqueous solution, simple and direct measurement, and less interference from the tested sample. It can also satisfactorily analyze the antioxidant structure-activity relationship. PTIO scavenging has no stereospecificity and is at least involved in H transfer. |
[Mh] Termos MeSH primário: |
Técnicas de Química Analítica/métodos Óxidos N-Cíclicos/química Depuradores de Radicais Livres/química Imidazóis/química
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[Mh] Termos MeSH secundário: |
Ácido Ascórbico/química Espectrometria de Massas Espectrofotometria Relação Estrutura-Atividade
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[Pt] Tipo de publicação: | EVALUATION STUDIES; JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Cyclic N-Oxides); 0 (Free Radical Scavengers); 0 (Imidazoles); 18390-00-6 (2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide); PQ6CK8PD0R (Ascorbic Acid) |
[Em] Mês de entrada: | 1708 |
[Cu] Atualização por classe: | 170811 |
[Lr] Data última revisão:
| 170811 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170711 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1021/acs.jafc.7b02247 |
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