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Pesquisa : D04.210.500.054.079.429.824.664 [Categoria DeCS]
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[PMID]:29016690
[Au] Autor:Wu GC; Li HW; Tey WG; Lin CJ; Chang CF
[Ad] Endereço:Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan.
[Ti] Título:Expression profile of amh/Amh during bi-directional sex change in the protogynous orange-spotted grouper Epinephelus coioides.
[So] Source:PLoS One;12(10):e0185864, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gonadal differentiation is tightly regulated by the initial sex determining gene and the downstream sex-related genes in vertebrates. However, sex change in fish can alter the sexual fate from one sex to the other. Chemical-induced maleness in the protogynous orange-spotted grouper is transient, and a reversible sex change occurs after the chemical treatment is withdrawn. We used these characteristics to study Amh signaling during bi-directional sex change in the grouper. We successfully induced the female-to-male sex change by chemical (aromatase inhibitor, AI, or methyltestosterone, MT) treatment. A dormant gonad (a low proliferation rate of early germ cells and no characteristics of both sexes) was found during the transient phase of reversible male-to-female sex change after the withdrawal of chemical administration. Our results showed that amh (anti-mullerian hormone) and its receptor amhr2 (anti-mullerian hormone receptor type 2) were significantly increased in the gonads during the process of female-to-male sex change. Amh is expressed in the Sertoli cells surrounding the type A spermatogonia in the female-to-male grouper. Male-related gene (dmrt1 and sox9) expression was immediately decreased in MT-terminated males during the reversible male-to-female sex change. However, Amh expression was found in the surrounding cells of type A spermatogonia-like cells during the transient phase of reversible male-to-female sex change. This phenomenon is correlated with the dormancy of type A spermatogonia-like cells. Thus, Amh signaling is suggested to play roles in regulating male differentiation during the female-to-male sex change and in inhibiting type-A spermatogonia-like cell proliferation/differentiation during the reversible male-to-female sex change. We suggest that Amh signaling might play dual roles during bi-directional sex change in grouper.
[Mh] Termos MeSH primário: Hormônio Antimülleriano/genética
Inibidores da Aromatase/farmacologia
Regulação da Expressão Gênica no Desenvolvimento
Metiltestosterona/farmacologia
Receptores de Peptídeos/genética
Receptores de Fatores de Crescimento Transformadores beta/genética
Diferenciação Sexual/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Hormônio Antimülleriano/metabolismo
Feminino
Masculino
Ovário/citologia
Ovário/efeitos dos fármacos
Ovário/metabolismo
Perciformes
Receptores de Peptídeos/metabolismo
Receptores de Fatores de Crescimento Transformadores beta/metabolismo
Fatores de Transcrição SOX9/genética
Fatores de Transcrição SOX9/metabolismo
Células de Sertoli/citologia
Células de Sertoli/efeitos dos fármacos
Células de Sertoli/metabolismo
Processos de Determinação Sexual
Diferenciação Sexual/genética
Transdução de Sinais
Espermatogônias/citologia
Espermatogônias/efeitos dos fármacos
Espermatogônias/metabolismo
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aromatase Inhibitors); 0 (DMRT1 protein); 0 (Receptors, Peptide); 0 (Receptors, Transforming Growth Factor beta); 0 (SOX9 Transcription Factor); 0 (Transcription Factors); 0 (anti-Mullerian hormone receptor); 80497-65-0 (Anti-Mullerian Hormone); V9EFU16ZIF (Methyltestosterone)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171011
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185864


  2 / 1007 MEDLINE  
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[PMID]:28717101
[Au] Autor:Kawamoto T; Ito Y; Morita O; Honda H
[Ad] Endereço:Safety Science Research, Kao Corporation.
[Ti] Título:Mechanism-based risk assessment strategy for drug-induced cholestasis using the transcriptional benchmark dose derived by toxicogenomics.
[So] Source:J Toxicol Sci;42(4):427-436, 2017.
[Is] ISSN:1880-3989
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Cholestasis is one of the major causes of drug-induced liver injury (DILI), which can result in withdrawal of approved drugs from the market. Early identification of cholestatic drugs is difficult due to the complex mechanisms involved. In order to develop a strategy for mechanism-based risk assessment of cholestatic drugs, we analyzed gene expression data obtained from the livers of rats that had been orally administered with 12 known cholestatic compounds repeatedly for 28 days at three dose levels. Qualitative analyses were performed using two statistical approaches (hierarchical clustering and principle component analysis), in addition to pathway analysis. The transcriptional benchmark dose (tBMD) and tBMD 95% lower limit (tBMDL) were used for quantitative analyses, which revealed three compound sub-groups that produced different types of differential gene expression; these groups of genes were mainly involved in inflammation, cholesterol biosynthesis, and oxidative stress. Furthermore, the tBMDL values for each test compound were in good agreement with the relevant no observed adverse effect level. These results indicate that our novel strategy for drug safety evaluation using mechanism-based classification and tBMDL would facilitate the application of toxicogenomics for risk assessment of cholestatic DILI.
[Mh] Termos MeSH primário: Clorpromazina/administração & dosagem
Clorpromazina/toxicidade
Colestase/induzido quimicamente
Ciclosporina/administração & dosagem
Ciclosporina/toxicidade
Diclofenaco/administração & dosagem
Diclofenaco/toxicidade
Medição de Risco/métodos
Toxicogenética/métodos
[Mh] Termos MeSH secundário: Administração Oral
Animais
Colesterol/biossíntese
Relação Dose-Resposta a Droga
Flutamida/administração & dosagem
Flutamida/toxicidade
Expressão Gênica
Seres Humanos
Imipramina/administração & dosagem
Imipramina/toxicidade
Inflamação/genética
Cetoconazol/administração & dosagem
Cetoconazol/toxicidade
Fígado
Metiltestosterona/administração & dosagem
Metiltestosterona/toxicidade
Estresse Oxidativo/genética
Ratos
Sulindaco/administração & dosagem
Sulindaco/toxicidade
Tamoxifeno/administração & dosagem
Tamoxifeno/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
094ZI81Y45 (Tamoxifen); 144O8QL0L1 (Diclofenac); 184SNS8VUH (Sulindac); 76W6J0943E (Flutamide); 83HN0GTJ6D (Cyclosporine); 97C5T2UQ7J (Cholesterol); OGG85SX4E4 (Imipramine); R9400W927I (Ketoconazole); U42B7VYA4P (Chlorpromazine); V9EFU16ZIF (Methyltestosterone)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170719
[St] Status:MEDLINE
[do] DOI:10.2131/jts.42.427


  3 / 1007 MEDLINE  
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[PMID]:28399454
[Au] Autor:Murray CM; Merchant M; Easter M; Padilla S; Garrigós DB; Sasa Marin M; Guyer C
[Ad] Endereço:Department of Biology, Tennessee Technological University, PO Box 5063, Cookeville, TN 38505, USA. Electronic address: cmmurray@tntech.edu.
[Ti] Título:Detection of a synthetic sex steroid in the American crocodile (Crocodylus acutus): Evidence for a novel environmental androgen.
[So] Source:Chemosphere;180:125-129, 2017 Aug.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Endocrine-disrupting contaminants (EDC's) are well known to alter sexual differentiation among vertebrates via estrogenic effects during development, particularly in organisms characterized by temperature-dependent sex determination. However, substances producing androgenic effects typically lack potency when tested in laboratory settings and are virtually unstudied in field settings. Here, we assay levels of a synthetic androgen, 17α-methyltestosterone (MT), in a heavily male-biased population of American crocodiles in the Tempisque River Basin of Costa Rica based on the recent hypothesis that this chemical is an EDC in developing crocodilian embryos. The presence of MT was documented in all field-collected samples of egg yolk and in plasma of all age classes in among population of crocodiles. Hatchlings exhibited higher plasma MT concentrations (102.1 ± 82.8 ng/mL) than juveniles (33.8 ± 51.5) and adults (25.9 ± 20.8 ng/mL). Among populations, crocodiles captured in the Tempisque River (62.9 ± 73.7 ng/mL) were higher in MT concentration than those from Tarcoles (13.3 ± 11.4 ng/mL) and negative controls (0.001 ± 0.0002 ng/mL). A mechanism for the bio-transport of MT and its subsequent effects is proposed.
[Mh] Termos MeSH primário: Jacarés e Crocodilos/metabolismo
Monitoramento Ambiental
Hormônios Esteroides Gonadais/metabolismo
Poluentes Químicos da Água/metabolismo
[Mh] Termos MeSH secundário: Androgênios
Animais
Costa Rica
Disruptores Endócrinos
Estrogênios
Feminino
Masculino
Metiltestosterona
Rios
Esteroides
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Androgens); 0 (Endocrine Disruptors); 0 (Estrogens); 0 (Gonadal Steroid Hormones); 0 (Steroids); 0 (Water Pollutants, Chemical); V9EFU16ZIF (Methyltestosterone)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE


  4 / 1007 MEDLINE  
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[PMID]:28322549
[Au] Autor:Abo-Al-Ela HG; El-Nahas AF; Mahmoud S; Ibrahim EM
[Ad] Endereço:Animal Health Research Institute , Shibin Al-Kom Branch, Agriculture Research Centre, El-Minufiya, Egypt.
[Ti] Título:Vitamin C Modulates the Immunotoxic Effect of 17α-Methyltestosterone in Nile Tilapia.
[So] Source:Biochemistry;56(14):2042-2050, 2017 Apr 11.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The synthetic androgen 17α-methyltestosterone (MT) is profusely used and practically needed in the production of all-male Nile tilapia fry; however, such androgenic hormones badly disrupt the immune system. This study aimed to alleviate or counteract the immunotoxic effect of MT using vitamin C (ascorbic acid or vit C). Our results show that the highest phagocytic activity (PA), phagocytic index (PI), and lysozyme activity were detected in the vit C group and the MT plus vit C group. Furthermore, PA and PI were significantly suppressed, but lysozyme activity was stronger in the MT group than in the control. No differences were detected in the differential leukocyte count among the studied groups. Moreover, vit C obviously reduced the upregulated expression level of the innate immune-related genes, interleukin 1ß (il1ß), interleukin 8 (il8), tumor necrosis factor α (tnfα), CC-chemokine, Toll-like receptor 7 (tlr7), immunoglobulin M (IgM) heavy chain, and cellular apoptosis susceptibility (cas) induced by MT, excluding tnfα in the liver and CC-chemokine and tlr7 in the kidney. The micronucleus frequency was found to significantly improve in the vit C plus MT group in comparison to that in the MT group. Normal histoarchitecture of the liver, kidney, and spleen was observed in all the groups, except for the frequently observed melanomacrophage centers in the spleen and kidney of the fish that were treated with vit C and vit C plus MT. More importantly, our findings demonstrate that the upregulation of immune-related genes is not necessarily a sign of a stimulated or enhanced immune system.
[Mh] Termos MeSH primário: Ácido Ascórbico/farmacologia
Disruptores Endócrinos/farmacologia
Proteínas de Peixes/imunologia
Imunidade Inata/efeitos dos fármacos
Metiltestosterona/antagonistas & inibidores
[Mh] Termos MeSH secundário: Animais
Ciclídeos
Proteína Substrato Associada a Crk/genética
Proteína Substrato Associada a Crk/imunologia
Proteínas de Peixes/genética
Regulação da Expressão Gênica
Imunoglobulina M/genética
Imunoglobulina M/imunologia
Interleucina-1beta/genética
Interleucina-1beta/imunologia
Interleucina-8/genética
Interleucina-8/imunologia
Rim/efeitos dos fármacos
Rim/imunologia
Rim/metabolismo
Contagem de Leucócitos
Leucócitos
Fígado/efeitos dos fármacos
Fígado/imunologia
Fígado/metabolismo
Masculino
Metiltestosterona/farmacologia
Testes para Micronúcleos
Muramidase/genética
Muramidase/imunologia
Fagocitose/efeitos dos fármacos
Baço/efeitos dos fármacos
Baço/imunologia
Baço/metabolismo
Receptor 7 Toll-Like/genética
Receptor 7 Toll-Like/imunologia
Fator de Necrose Tumoral alfa/genética
Fator de Necrose Tumoral alfa/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Crk-Associated Substrate Protein); 0 (Endocrine Disruptors); 0 (Fish Proteins); 0 (Immunoglobulin M); 0 (Interleukin-1beta); 0 (Interleukin-8); 0 (Toll-Like Receptor 7); 0 (Tumor Necrosis Factor-alpha); EC 3.2.1.17 (Muramidase); PQ6CK8PD0R (Ascorbic Acid); V9EFU16ZIF (Methyltestosterone)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.6b01284


  5 / 1007 MEDLINE  
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[PMID]:28275215
[Au] Autor:Li Z; Liu X; Sun Y; Liu J; Liu Y; Wang M; Zhang Q; Wang X
[Ad] Endereço:Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China. lizanlxm@163.com.
[Ti] Título:Roles of GATA6 during Gonadal Development in Japanese Flounder: Gonadogenesis, Regulation of Gender-Related Genes, Estrogen Formation and Gonadal Function Maintenance.
[So] Source:Int J Mol Sci;18(1), 2017 Jan 16.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:GATA-binding protein 6 (GATA6), a highly-conserved transcription factor of the GATA family plays an important role in gonadal cell proliferation, differentiation and endoderm development. In this study, the full-length cDNA of GATA6 of (Japanese flounder) was obtained. Phylogenetic, gene structure and synteny analyses demonstrated that GATA6 of is homologous to that of teleosts and tetrapods. The GATA6 transcript showed higher expression in testis than in ovary, demonstrating a sexually dimorphic gene expression. During embryonic development, the expression of GATA6 increased at the blastula stage, demonstrating that GATA6 is involved in morphogenesis. Results of in situ hybridization showed that GATA6 signals were detected in Sertoli cells, oogonia and oocytes. Moreover, 17α methyl testosterone, a male hormone, could moderately upregulate GATA6 and downregulate aromatase CYP19A1 in testis cells. These results suggest that GATA6 may play an important role in gonadal development in . This study provides valuable information on the function of GATA6, laying the foundation for further development of breeding techniques in this species.
[Mh] Termos MeSH primário: Estrogênios/metabolismo
Linguado/embriologia
Linguado/genética
Fator de Transcrição GATA6/metabolismo
Gônadas/embriologia
Caracteres Sexuais
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Aromatase/genética
Aromatase/metabolismo
Sequência de Bases
Células Cultivadas
Cromossomos/genética
DNA Complementar/genética
DNA Complementar/isolamento & purificação
Desenvolvimento Embrionário/efeitos dos fármacos
Desenvolvimento Embrionário/genética
Feminino
Fator de Transcrição GATA6/química
Fator de Transcrição GATA6/genética
Perfilação da Expressão Gênica
Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos
Genoma
Gônadas/efeitos dos fármacos
Gônadas/metabolismo
Hibridização In Situ
Masculino
Metiltestosterona/farmacologia
Filogenia
Domínios Proteicos
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Alinhamento de Sequência
Homologia Estrutural de Proteína
Sintenia
Testículo/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Estrogens); 0 (GATA6 Transcription Factor); 0 (RNA, Messenger); EC 1.14.14.1 (Aromatase); V9EFU16ZIF (Methyltestosterone)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170424
[Lr] Data última revisão:
170424
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170310
[St] Status:MEDLINE


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[PMID]:28244568
[Au] Autor:Forte R; Pesce C; De Vito G; Boreham CA
[Ad] Endereço:Roberta Forte Department of Human Health and Movement Sciences, University of Rome «Foro Italico¼, Roma 00135, Italy telephone +39 6 36733367 fax +39 6 36733362 e-mail: roberta.forte@uniroma4.it.
[Ti] Título:The Body Fat-Cognition Relationship in Healthy Older Individuals: Does Gynoid vs Android Distribution Matter?
[So] Source:J Nutr Health Aging;21(3):284-291, 2017.
[Is] ISSN:1760-4788
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To examine the relationship between regional and whole body fat accumulation and core cognitive executive functions. DESIGN: Cross-sectional study. SETTINGS AND PARTICIPANTS: 78 healthy men and women aged between 65 and 75 years recruited through consumer's database. MEASUREMENTS: DXA measured percentage total body fat, android, gynoid distribution and android/gynoid ratio; inhibition and working memory updating through Random Number Generation test and cognitive flexibility by Trail Making test. First-order partial correlations between regional body fat and cognitive executive function were computed partialling out the effects of whole body fat. Moderation analysis was performed to verify the effect of gender on the body fat-cognition relationship. RESULTS: Results showed a differentiated pattern of fat-cognition relationship depending on fat localization and type of cognitive function. Statistically significant relationships were observed between working memory updating and: android fat (r = -0.232; p = 0.042), gynoid fat (r = 0.333; p = 0.003) and android/gynoid ratio (r = -0.272; p = 0.017). Separating genders, the only significant relationship was observed in females between working memory updating and gynoid fat (r = 0.280; p = 0.045). In spite of gender differences in both working memory updating and gynoid body fat levels, moderation analysis did not show an effect of gender on the relationship between gynoid fat and working memory updating. CONCLUSIONS: Results suggest a protective effect of gynoid body fat and a deleterious effect of android body fat. Although excessive body fat increases the risk of developing CDV, metabolic and cognitive problems, maintaining a certain proportion of gynoid fat may help prevent cognitive decline, particularly in older women. Guidelines for optimal body composition maintenance for the elderly should not target indiscriminate weight loss, but weight maintenance through body fat/lean mass control based on non-pharmacological tools such as physical exercise, known to have protective effects against CVD risk factors and age-related cognitive deterioration.
[Mh] Termos MeSH primário: Tecido Adiposo/metabolismo
Distribuição da Gordura Corporal
Cognição/fisiologia
Disfunção Cognitiva/fisiopatologia
Função Executiva/fisiologia
Obesidade/metabolismo
[Mh] Termos MeSH secundário: Absorciometria de Fóton/métodos
Idoso
Antropometria/métodos
Índice de Massa Corporal
Peso Corporal
Estudos Transversais
Exercício
Feminino
Seres Humanos
Masculino
Metiltestosterona/sangue
Fatores de Risco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
V9EFU16ZIF (Methyltestosterone)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170301
[St] Status:MEDLINE
[do] DOI:10.1007/s12603-016-0783-1


  7 / 1007 MEDLINE  
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[PMID]:28243861
[Au] Autor:He Y; Fang J; Xue L; Wu J; Dawar FU; Mei J
[Ad] Endereço:College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Huazhong Agricultural University, Wuhan, 430070, China. heyan@mail.hzau.edu.cn.
[Ti] Título:Potential contributions of heat shock proteins and related genes in sexual differentiation in yellow catfish (Pelteobagrus fulvidraco).
[So] Source:Fish Physiol Biochem;43(2):465-475, 2017 Apr.
[Is] ISSN:1573-5168
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Sex determination and differentiation in ectotherms are very complicated affairs and usually affected by both genetic and environmental factors. Because of their temperature-sensitive expression, heat shock proteins (HSPs) are good candidates for temperature-dependent sex determination (TSD). Similar to most thermosensitive fish species, the male to female ratio increases with temperature in yellow catfish (Pelteobagrus fulvidraco). Yellow catfish is also a type of sexual size dimorphic fish, and the male individuals grow much faster than females of the same age. Therefore, research of sex differentiation in yellow catfish is important in aquiculture. In this attempt, a total of seven HSPs and related genes were identified from transcriptomes of yellow catfish by 454 pyrosequencing and Solexa sequencing that we did previously, including five genes with complete open reading frame (ORF). Phylogenetically, all these genes were compared with their counterparts from other vertebrates. All these genes were sex-biased expressed in gonads. Hspa5, Hip, and Cdc37 were expressed more highly in ovary than in testis, whereas Hsp90α, Hspb2, Hspb8, and Hspbp1 were expressed more highly in testis than in ovary. Additionally, the expression of these genes was assessed after 17α-methyltestosterone (MT) and 17α-ethinylestradiol (EE2) treatment, respectively. Our result showed that working as co-chaperones, these HSPs and related genes may regulate sex steroid receptor activities to influence gonad development in yellow catfish. Our work would help in the understanding of the mechanism of sexual differentiation in teleosts.
[Mh] Termos MeSH primário: Peixes-Gato/genética
Proteínas de Peixes/genética
Proteínas de Choque Térmico/genética
Diferenciação Sexual/genética
[Mh] Termos MeSH secundário: Animais
Peixes-Gato/fisiologia
Etinilestradiol/farmacologia
Feminino
Masculino
Metiltestosterona/farmacologia
Ovário/efeitos dos fármacos
Ovário/metabolismo
Filogenia
Testículo/efeitos dos fármacos
Testículo/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); 0 (Heat-Shock Proteins); 423D2T571U (Ethinyl Estradiol); V9EFU16ZIF (Methyltestosterone)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170301
[St] Status:MEDLINE
[do] DOI:10.1007/s10695-016-0303-6


  8 / 1007 MEDLINE  
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[PMID]:27902922
[Au] Autor:Abo-Al-Ela HG; El-Nahas AF; Mahmoud S; Ibrahim EM
[Ad] Endereço:Animal Health Research Institute, Shibin Al-Kom Branch, Agriculture Research Centre, El-Minufiya, Egypt; Department of Animal Wealth Development, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt. Electronic address: aboalela@ahri.gov.eg.
[Ti] Título:The extent to which immunity, apoptosis and detoxification gene expression interact with 17 alpha-methyltestosterone.
[So] Source:Fish Shellfish Immunol;60:289-298, 2017 Jan.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Innate immunity is the first line of defence against invasion by foreign pathogens. One widely used synthetic androgen for the production of all-male fish, particularly commercially valuable Nile tilapia, Oreochromis niloticus, is 17 alpha-methyltestosterone (MT). The present study investigates the effect of MT on innate immunity, cellular apoptosis and detoxification and the mortality rate, during and after the feeding of fry with 0-, 40-and 60-mg MT/kg. Expression analysis was completed on interleukin 1 beta (il1ß), interleukin 8 (il8), tumour necrosis factor alpha (tnfα), CXC2- and CC-chemokines, interferon (ifn), myxovirus resistance (mx), toll-like receptor 7 (tlr7), immunoglobulin M heavy chain (IgM heavy chain), vitellogenin (vtg), cellular apoptosis susceptibility (cas) and glutathione S-transferase α1 (gstα1). Expression analysis revealed that MT had a significant impact on these genes, and this impact varied from induction to repression during and after the treatment. Linear regression analysis showed a significant association between the majority of the tested gene transcript levels and mortality rates on the 7 and 21 days of hormonal treatment and 2 weeks following hormonal cessation. The results are thoroughly discussed in this article. This is the first report concerning the hazardous effect of MT on a series of genes involved in immunity, apoptosis and detoxification in the Nile tilapia fry.
[Mh] Termos MeSH primário: Apoptose
Ciclídeos/genética
Ciclídeos/imunologia
Imunidade Inata
[Mh] Termos MeSH secundário: Animais
Ciclídeos/metabolismo
Relação Dose-Resposta a Droga
Proteínas de Peixes/genética
Proteínas de Peixes/metabolismo
Expressão Gênica
Inativação Metabólica
Longevidade
Masculino
Metiltestosterona/metabolismo
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); 0 (RNA, Messenger); V9EFU16ZIF (Methyltestosterone)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170324
[Lr] Data última revisão:
170324
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE


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[PMID]:27344345
[Au] Autor:Vatakuti S; Olinga P; Pennings JL; Groothuis GM
[Ad] Endereço:Division of Pharmacokinetics, Toxicology and Targeting, Groningen Research Institute for Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV, Groningen, The Netherlands.
[Ti] Título:Validation of precision-cut liver slices to study drug-induced cholestasis: a transcriptomics approach.
[So] Source:Arch Toxicol;91(3):1401-1412, 2017 Mar.
[Is] ISSN:1432-0738
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Hepatotoxicity is one of the major reasons for withdrawal of drugs from the market. Therefore, there is a need to screen new drugs for hepatotoxicity in humans at an earlier stage. The aim of this study was to validate human precision-cut liver slices (PCLS) as an ex vivo model to predict drug-induced cholestasis and identify the possible mechanisms of cholestasis-induced toxicity using gene expression profiles. Five hepatotoxicants, which are known to induce cholestasis (alpha-naphthyl isothiocyanate, chlorpromazine, cyclosporine, ethinyl estradiol and methyl testosterone) were used at concentrations inducing low (<30 %) and medium (30-50 %) toxicity, based on ATP content. Human PCLS were incubated with the drugs in the presence of a non-toxic concentration (60 µM) of a bile acid mixture (portal vein concentration and composition) as model for bile acid-induced cholestasis. Regulated genes include bile acid transporters and cholesterol transporters. Pathway analysis revealed that hepatic cholestasis was among the top ten regulated pathways, and signaling pathways such as farnesoid X receptor- and liver X receptor-mediated responses, which are known to play a role in cholestasis, were significantly affected by all cholestatic compounds. Other significantly affected pathways include unfolded protein response and protein ubiquitination implicating the role of endoplasmic reticulum stress. This study shows that human PCLS incubated in the presence of a physiological bile acid mixture correctly reflect the pathways affected in drug-induced cholestasis in the human liver. In the future, this human PCLS model can be used to identify cholestatic adverse drug reactions of new chemical entities.
[Mh] Termos MeSH primário: Colestase/induzido quimicamente
Fígado/efeitos dos fármacos
[Mh] Termos MeSH secundário: 1-Naftilisotiocianato/toxicidade
Idoso
Clorpromazina/efeitos adversos
Colestase/genética
Ciclosporina/efeitos adversos
Relação Dose-Resposta a Droga
Etinilestradiol/efeitos adversos
Feminino
Perfilação da Expressão Gênica/métodos
Seres Humanos
Masculino
Metiltestosterona/efeitos adversos
Meia-Idade
Transdução de Sinais/efeitos dos fármacos
Transdução de Sinais/genética
Transcriptoma/efeitos dos fármacos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
423D2T571U (Ethinyl Estradiol); 551-06-4 (1-Naphthylisothiocyanate); 83HN0GTJ6D (Cyclosporine); U42B7VYA4P (Chlorpromazine); V9EFU16ZIF (Methyltestosterone)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170306
[Lr] Data última revisão:
170306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160627
[St] Status:MEDLINE
[do] DOI:10.1007/s00204-016-1778-8


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[PMID]:27802873
[Au] Autor:Fatima S; Adams M; Wilkinson R
[Ad] Endereço:Institute for Marine and Antarctic Studies (IMAS), University of Tasmania, Locked Bag 1370, Launceston, Australia. Electronic address: shafaq.fatima2013@gmail.com.
[Ti] Título:Sex reversal of brook trout (Salvelinus fontinalis) by 17α-methyltestosterone exposure: A serial experimental approach to determine optimal timing and delivery regimes.
[So] Source:Anim Reprod Sci;175:39-47, 2016 Dec.
[Is] ISSN:1873-2232
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Commercial culture of Brook trout (Salvelinus fontinalis) in Tasmania was partly abandoned due to sexual maturation of male fish early on during the estuarine rearing phase. Maturation adversely affects body mass, flesh quality and immunocompetency effectively. Sex reversal techniques such as the in-feed addition of a synthetic androgen have proven difficult to adapt in brook trout. An appropriate timing, duration and delivery vehicle for administration of 17α-methyltestosterone (MT) to produce phenotypic males (neomales) from genotypically female brook trout required further investigation. In this study, groups of brook trout eggs (n=1000) maintained at 9.5±0.15-10±0.14°C, were immersed in MT (400µgL ) for four hours on two alternate days (two immersions/group) staggered over a two week period surrounding the hatch of embryos (control groups excluded). The groups were then split and half received MT-supplemented feed for 60days and the other a standard diet. Following an 11 month on-growing period sex phenotypes were determined by gross & histological gonad morphology. The highest proportion of male phenotypes (75%) was found in fish immersed six and four days pre-hatch and subsequently fed a normal diet. Fish fed a MT supplemented diet and immersed in MT showed significantly higher proportions of sterile fish. These data indicate that a pre-hatch immersion-only regime (4-6days pre-hatch at 9.5°C) should be pursued as a target for optimization studies to further refine the effective concentration and duration of exposure to MT for the successful production of neo-male brook trout.
[Mh] Termos MeSH primário: Transtornos do Desenvolvimento Sexual/induzido quimicamente
Metiltestosterona/farmacologia
Truta/fisiologia
[Mh] Termos MeSH secundário: Ração Animal
Animais
Esquema de Medicação
Feminino
Gônadas/efeitos dos fármacos
Gônadas/fisiologia
Masculino
Análise para Determinação do Sexo
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
V9EFU16ZIF (Methyltestosterone)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170118
[Lr] Data última revisão:
170118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161103
[St] Status:MEDLINE



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