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[PMID]:29202363
[Au] Autor:Guo C; Xie C; Ding P; Qin G; Mo W; Cao X; Zheng S
[Ad] Endereço:Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education), The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China. Electronic address: cheng_guo@zju.edu.cn.
[Ti] Título:Quantification of glycocholic acid in human serum by stable isotope dilution ultra performance liquid chromatography electrospray ionization tandem mass spectrometry.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1072:315-319, 2018 Jan 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A rapid, accurate and sensitive stable isotope dilution ultra performance liquidchromatography electrospray ionization tandem mass spectrometry (ID-UPLC-ESI-MS/MS) method for the determination of glycocholic acid (GCA) in human serum was developed and validated. Serum samples were spiked with D -glycocholic acid and then pretreated with protein precipitation. The analysis was performed on a Waters BEH C18 column (100 mm×2.1mm, 1.7µm), followed by ESI-MS/MS detection in negative ion mode under multiple reaction monitoring mode. The calibration curves covered a concentration range from 0.2 to 400ng/mL. The limit of detection and limit of quantification was 0.01ng/mL and 0.05ng/mL, respectively. The method showed satisfactory precision on intra-day (2.3-6.1%) and inter-day (2.4-4.6%) analyses and achieved good recovery at three spiked levels (103.7-114.3%). Moreover, this established method was successfully applied for quantification of GCA in serum samples from healthy volunteers, patients with hepatocellular carcinoma (HCC) and patients with other cancers. We demonstrated that the level of GCA in patients with HCC was significantly higher not only than that in healthy controls, but also than that in patients with other cancer, whereas no significant difference of GCA level was observed between healthy control group and other cancers group.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Ácido Glicocólico/sangue
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Biomarcadores Tumorais/sangue
Carcinoma Hepatocelular/sangue
Feminino
Seres Humanos
Limite de Detecção
Modelos Lineares
Neoplasias Hepáticas/sangue
Masculino
Meia-Idade
Neoplasias/sangue
Reprodutibilidade dos Testes
Espectrometria de Massas por Ionização por Electrospray/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); G59NX3I3RT (Glycocholic Acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE


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[PMID]:28668637
[Au] Autor:D'Onofrio M; Barracchia CG; Bortot A; Munari F; Zanzoni S; Assfalg M
[Ad] Endereço:Department of Biotechnology, University of Verona, 37134 Verona, Italy.
[Ti] Título:Molecular differences between human liver fatty acid binding protein and its T94A variant in their unbound and lipid-bound states.
[So] Source:Biochim Biophys Acta;1865(9):1152-1159, 2017 09.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Liver fatty acid binding protein (L-FABP) is an abundant cytosolic protein playing a central role in intracellular lipid trafficking. The L-FABP T94A variant, originating from one of the most common polymorphisms in the FABP family, is associated with several lipid-related disorders. However, the molecular factors that determine the observed functional differences are currently unknown. In our work, we performed a high resolution comparative molecular analysis of L-FABP T94T and L-FABP T94A in their unbound states and in the presence of representative ligands of the fatty acid and bile acid classes. We collected residue-resolved NMR spectral fingerprints of the two variants, and compared secondary structures, backbone dynamics, side chain arrangements, binding site occupation, and intermolecular contacts. We found that threonine to alanine replacement did not result in strongly perturbed structural and dynamic features, although differences in oleic acid binding by the two variants were detected. Based on chemical shift perturbations at sites distant from position 94 and on differences in intermolecular contacts, we suggest that long-range communication networks in L-FABP propagate the effect of amino acid substitution at sites relevant for ligand binding or biomolecular recognition.
[Mh] Termos MeSH primário: Proteínas de Ligação a Ácido Graxo/química
Ácido Glicocólico/metabolismo
Ácido Oleico/metabolismo
Polimorfismo de Nucleotídeo Único
[Mh] Termos MeSH secundário: Regulação Alostérica
Substituição de Aminoácidos
Sítios de Ligação
Proteínas de Ligação a Ácido Graxo/genética
Seres Humanos
Modelos Moleculares
Ressonância Magnética Nuclear Biomolecular
Ligação Proteica
Conformação Proteica
Proteínas Recombinantes/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (FABP1 protein, human); 0 (Fatty Acid-Binding Proteins); 0 (Recombinant Proteins); 2UMI9U37CP (Oleic Acid); G59NX3I3RT (Glycocholic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170703
[St] Status:MEDLINE


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[PMID]:28647962
[Au] Autor:Yu Y; Zhou CL; Yu TT; Han XJ; Shi HY; Wang HZ; Shen JJ; He J
[Ad] Endereço:Department of Obstetrics, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
[Ti] Título:[Effect of endoplasmic reticulum stress in trophocytes on the pathogenesis of intrahepatic cholestasis of pregnancy].
[So] Source:Zhonghua Fu Chan Ke Za Zhi;52(6):392-397, 2017 Jun 25.
[Is] ISSN:0529-567X
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To evaluate the effect of endoplasmic reticulum stress in trophocytes, in patients with intrahepatic cholestasis of pregnancy (ICP). Sixty-one pregnant women who were hospitalized in Women's Hospital, School of Medicine, Zhejiang University from January to December 2015 were recruited. Thirty-one women who were diagnosed as ICP were defined as the ICP group and 30 healthy pregnant women were defined as the control group. The localization and expression intensity of glucose regulated protein 78 (GRP-78) in placental tissues were detected by immunohistochemistry technique. Electronic microscope was used to observe ultra-microstructure change of the endoplasmic reticulum in trophocytes and cell line Swan71. Reverse transcription (RT)-PCR and western blot were used to investigate the expression of GRP-78 mRNA and protein in Swan 71 cell. (1) GRP-78 protein was mainly expressed in the cytoplasm of cytotrophoblasts and syncytiotrophoblasts. The protein expression of GRP-78 in placentas of the ICP group (13.2±2.4) was significantly higher than that in the control group (7.8±1.3, 0.01). (2) The volume of endoplasmie reticulum did not increase and the microvilli developed well, with no swelling and no expansion of endoplasmic reticulum in the control group.In the ICP group, microvilli injury, endoplasmic reticulum edema were found; the volume of endoplasmic reticulum increased, with dilation, vacuolation and significant degranulation. After treated with 100 µmol/L cholyglycine for 24 hours, universal dilatation of the endoplasmic reticulum were seen in the Swan71 cells. (3) In Swan71 cells, cholylglycine displayed a concentration-dependent up-regulation on the expression of GRP-78. The expressions of GRP-78 mRNA in 0, 25, 50, 100 µmol/L cholylglycine experimental group were 1.01±0.17, 2.17±0.16, 5.47±0.36, 5.65±0.82, respectively. The expression of GRP-78 protein in 0, 25, 50, 100 µmol/L cholylglycine experimental group were 1.01±0.04, 1.17±0.15, 1.33±0.13, 1.73±0.13, respectively. The expression of GRP-78 mRNA and protein in 100 and 50 µmol/L cholylglycine experimental group were significantly higher than 0 µmol/L (all 0.01). The obvious expansion of endoplasmic reticulum and the increased expression of GRP-78 in trophocytes indicated that endoplasmic reticulum stress of trophocytes may be involved in the pathogenesis of ICP.
[Mh] Termos MeSH primário: Colestase Intra-Hepática/patologia
Estresse do Retículo Endoplasmático
Retículo Endoplasmático/metabolismo
Proteínas de Choque Térmico/genética
Placenta/metabolismo
Complicações na Gravidez/patologia
[Mh] Termos MeSH secundário: Animais
Western Blotting
Estudos de Casos e Controles
Feminino
Ácido Glicocólico
Proteínas de Choque Térmico/metabolismo
Seres Humanos
Gravidez
Terceiro Trimestre da Gravidez
RNA Mensageiro/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Trofoblastos
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Heat-Shock Proteins); 0 (RNA, Messenger); 0 (molecular chaperone GRP78); G59NX3I3RT (Glycocholic Acid)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170627
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.0529-567X.2017.06.007


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[PMID]:28591793
[Au] Autor:Thöni V; Pfister A; Melmer A; Enrich B; Salzmann K; Kaser S; Lamina C; Ebenbichler CF; Hackl H; Tilg H; Moschen AR
[Ad] Endereço:Department of Medicine, Division of Internal Medicine I (Gastroenterology, Endocrinology, and Metabolism), Medical University of Innsbruck, Innsbruck A-6020, Austria.
[Ti] Título:Dynamics of Bile Acid Profiles, GLP-1, and FGF19 After Laparoscopic Gastric Banding.
[So] Source:J Clin Endocrinol Metab;102(8):2974-2984, 2017 Aug 01.
[Is] ISSN:1945-7197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Context: An increase of bile acids (BAs), fibroblast growth factor 19 (FGF19), and glucagon-like peptide 1 (GLP-1) has been implicated in metabolic improvements after Roux-en-Y gastric bypass and vertical sleeve gastrectomy. However, data are still conflicting regarding their role after laparoscopic adjustable gastric banding (LAGB). Objective: To assess the fasting BA, FGF19, and GLP-1 concentrations in plasma before and after LAGB and to test for correlations with immunometabolic parameters. Furthermore, hepatic farnesoid X receptor (FXR) expression and regulation of FXR-dependent genes were analyzed. Design and Setting: Observational study at the University Hospital Innsbruck. Patients: Twenty obese patients. Interventions: Fasting plasma samples were taken before, 3, 6, and 12 months after LAGB. Liver biopsies were obtained at surgery and after 6 months postoperatively. Main Outcome Measures: BA profiles, GLP-1 and FGF19 levels, hepatic FXR expression and regulation of FXR target genes were determined. Results: Total, conjugated, and secondary BAs transiently increased 3 months after LAGB (P < 0.01). Only one BA, glycolithocholic acid sulfate, remained significantly elevated throughout the whole follow-up period (P < 0.05). GLP-1 had increased transiently 3 months after surgery (P < 0.01), whereas FGF19 levels increased continuously (P < 0.05). Insulin, homeostasis model assessment index, C-reactive protein, FGF19, and GLP-1 correlated positively with different BAs. No differences were seen in hepatic FXR expression and FXR-regulated genes. Conclusions: Our study results, not only identified LAGB-induced changes in BAs and BA-induced hormones, but also revealed associations between changes in BA profile with GLP-1 and FGF19.
[Mh] Termos MeSH primário: Ácidos e Sais Biliares/sangue
Fatores de Crescimento de Fibroblastos/sangue
Peptídeo 1 Semelhante ao Glucagon/sangue
Fígado/metabolismo
Obesidade Mórbida/sangue
Receptores Citoplasmáticos e Nucleares/metabolismo
[Mh] Termos MeSH secundário: Adulto
Cirurgia Bariátrica
Proteína C-Reativa/metabolismo
Feminino
Regulação da Expressão Gênica
Ácido Glicocólico/análogos & derivados
Ácido Glicocólico/sangue
Seres Humanos
Imuno-Histoquímica
Insulina/sangue
Resistência à Insulina
Laparoscopia
Masculino
Obesidade Mórbida/cirurgia
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Nm] Nome de substância:
0 (Bile Acids and Salts); 0 (FGF19 protein, human); 0 (Insulin); 0 (Receptors, Cytoplasmic and Nuclear); 0 (farnesoid X-activated receptor); 15324-64-8 (sulfolithocholylglycine); 62031-54-3 (Fibroblast Growth Factors); 89750-14-1 (Glucagon-Like Peptide 1); 9007-41-4 (C-Reactive Protein); G59NX3I3RT (Glycocholic Acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE
[do] DOI:10.1210/jc.2017-00235


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[PMID]:28538412
[Au] Autor:Yu XH; Sun J; Wang Y; Zhou YB
[Ad] Endereço:Second Department of Cardiovascular, The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, China.
[Ti] Título:Biomarkers of unstable angina pectoris and yangxin decoction intervention: An exploratory metabonomics study of blood plasma.
[So] Source:Medicine (Baltimore);96(21):e6998, 2017 May.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: This study aimed to explore the related metabolic biomarkers and to observe the effects of Yangxin Decoction (YXD) on plasma metabolism of patients with unstable angina (UA). METHODS: In total, 10 patients with UA (intervention group) and 10 healthy participants (control group) were recruited for this study from January 2009 to December 2010. Plasma samples from both groups were analyzed using liquid chromatography mass spectrometry (LC-MS). Principle component analysis (PCA) and partial least squares (PLS) were used to explore the correlations between metabolic markers in patients with UA. RESULTS: The LC-MS results indicated that the serum levels of 5 potential metabolic markers, namely, ceramide, glycocholic acid, allocholic acid, lithocholic acid, and leukotriene (LT) B4, were significantly higher in the intervention group than those in the control group. CONCLUSION: The results of this study demonstrated potential metabolic markers that can be used to distinguish and diagnose patients with UA.
[Mh] Termos MeSH primário: Angina Instável/sangue
Angina Instável/tratamento farmacológico
Medicamentos de Ervas Chinesas/uso terapêutico
[Mh] Termos MeSH secundário: Administração Oral
Biomarcadores/sangue
Análise Química do Sangue
Ceramidas/sangue
Ácidos Cólicos/sangue
Cromatografia Líquida
Feminino
Ácido Glicocólico/sangue
Seres Humanos
Leucotrieno B4/sangue
Ácido Litocólico/sangue
Masculino
Espectrometria de Massas
Metabolômica
Meia-Idade
Análise de Componente Principal
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Ceramides); 0 (Cholic Acids); 0 (Drugs, Chinese Herbal); 0 (yangxin decoction); 1HGW4DR56D (Leukotriene B4); 2464-18-8 (allocholic acid); 5QU0I8393U (Lithocholic Acid); G59NX3I3RT (Glycocholic Acid)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170629
[Lr] Data última revisão:
170629
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170525
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000006998


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[PMID]:27601896
[Au] Autor:Ling G; Zhang T; Zhang P; Sun J; He Z
[Ad] Endereço:Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, People's Republic of China.
[Ti] Título:Synergistic and complete reversal of the multidrug resistance of mitoxantrone hydrochloride by three-in-one multifunctional lipid-sodium glycocholate nanocarriers based on simultaneous BCRP and Bcl-2 inhibition.
[So] Source:Int J Nanomedicine;11:4077-91, 2016.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:Multidrug resistance (MDR) is a severe obstacle to successful chemotherapy due to its complicated nature that involves multiple mechanisms, such as drug efflux by transporters (P-glycoprotein and breast cancer resistance protein, BCRP) and anti-apoptotic defense (B-cell lymphoma, Bcl-2). To synergistically and completely reverse MDR by simultaneous inhibition of pump and non-pump cellular resistance, three-in-one multifunctional lipid-sodium glycocholate (GcNa) nanocarriers (TMLGNs) have been designed for controlled co-delivery of water-soluble cationic mitoxantrone hydrochloride (MTO), cyclosporine A (CsA - BCRP inhibitor), and GcNa (Bcl-2 inhibitor). GcNa and dextran sulfate were incorporated as anionic compounds to enhance the encapsulation efficiency of MTO (up to 97.8%±1.9%) and sustain the release of cationic MTO by electrostatic interaction. The results of a series of in vitro and in vivo investigations indicated that the TMLGNs were taken up by the resistant cancer cells by an endocytosis pathway that escaped the efflux induced by BCRP, and the simultaneous release of CsA with MTO further efficiently inhibited the efflux of the released MTO by BCRP; meanwhile GcNa induced the apoptosis process, and an associated synergistic antitumor activity and reversion of MDR were achieved because the reversal index was almost 1.0.
[Mh] Termos MeSH primário: Membro 2 da Subfamília G de Transportadores de Cassetes de Ligação de ATP/antagonistas & inibidores
Resistência a Medicamentos Antineoplásicos/efeitos dos fármacos
Ácido Glicocólico/administração & dosagem
Lipídeos/química
Mitoxantrona/administração & dosagem
Proteínas de Neoplasias/antagonistas & inibidores
Proteínas Proto-Oncogênicas c-bcl-2/antagonistas & inibidores
[Mh] Termos MeSH secundário: Transportadores de Cassetes de Ligação de ATP/metabolismo
Membro 1 da Subfamília B de Cassetes de Ligação de ATP/metabolismo
Animais
Antineoplásicos/farmacologia
Apoptose
Linhagem Celular Tumoral
Clatrina/química
Resistência a Múltiplos Medicamentos/efeitos dos fármacos
Endocitose
Seres Humanos
Concentração Inibidora 50
Lipídeos/farmacologia
Linfoma de Células B/tratamento farmacológico
Células MCF-7
Masculino
Tamanho da Partícula
Ratos
Ratos Wistar
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ABCG2 protein, human); 0 (ATP Binding Cassette Transporter, Sub-Family G, Member 2); 0 (ATP-Binding Cassette, Sub-Family B, Member 1); 0 (Antineoplastic Agents); 0 (BCL2 protein, human); 0 (Clathrin); 0 (Lipids); 0 (Neoplasm Proteins); 0 (Proto-Oncogene Proteins c-bcl-2); BZ114NVM5P (Mitoxantrone); G59NX3I3RT (Glycocholic Acid)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160908
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S95767


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[PMID]:27519826
[Au] Autor:Maroni A; Del Curto MD; Salmaso S; Zema L; Melocchi A; Caliceti P; Gazzaniga A
[Ad] Endereço:Università degli Studi di Milano, Dipartimento di Scienze Farmaceutiche, Sezione di Tecnologia e Legislazione Farmaceutiche "M.E. Sangalli", Via G. Colombo 71, 20133 Milano, Italy.
[Ti] Título:In vitro and in vivo evaluation of an oral multiple-unit formulation for colonic delivery of insulin.
[So] Source:Eur J Pharm Biopharm;108:76-82, 2016 Nov.
[Is] ISSN:1873-3441
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A multiple-unit formulation for time-dependent colonic release of insulin was obtained by coating insulin and sodium glycocholate immediate-release minitablets with: (i) Methocel® E50, a low-viscosity hydroxypropyl methylcellulose (inner coating), (ii) 5:1 w/w Eudragit® NE/Explotab® V17, a mixture of a neutral polymethacrylate with a pore-forming superdisintegrant (intermediate coating), and (iii) Aqoat® AS, enteric-soluble hydroxypropyl methylcellulose acetate succinate (outer coating). Sodium glycocholate was added as a permeation enhancer while the inner, intermediate and outer coatings were aimed, respectively, at delaying the onset of release through swelling/erosion processes, extending the duration of the lag phase by slowing down water penetration into the underlying functional layer, and overcoming variable gastric residence time. In vitro studies showed that neither insulin nor sodium glycocholate were released from the three-layer system during 2h of testing in 0.1N HCl, while complete release of the protein and of the enhancer occurred in phosphate buffer, pH 6.8, after consistent lag phases. No significant changes were noticed in the release profiles following twelve-month storage at 4°C. Oral administration of the novel formulation to diabetic rats elicited a peak in the plasma insulin concentration after 6h, which was associated with a sharp decrease in the glycemic levels. The relative bioavailability and pharmacological availability of such a formulation, as determined vs. the uncoated tablets, were 2.2 and 10.3, respectively. Based on these results, the three-layer system presented was considered a potentially interesting tool for oral colonic delivery of insulin and adjuvant compounds.
[Mh] Termos MeSH primário: Colo/efeitos dos fármacos
Preparações de Ação Retardada/química
Insulina/administração & dosagem
Metilcelulose/química
[Mh] Termos MeSH secundário: Adjuvantes Farmacêuticos
Administração Oral
Animais
Área Sob a Curva
Glicemia/química
Tampões (Química)
Bovinos
Diabetes Mellitus Experimental/tratamento farmacológico
Portadores de Fármacos/química
Sistemas de Liberação de Medicamentos
Ácido Glicocólico/química
Concentração de Íons de Hidrogênio
Masculino
Microscopia Eletrônica de Varredura
Ratos
Ratos Sprague-Dawley
Sódio/química
Comprimidos
Temperatura Ambiente
Viscosidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adjuvants, Pharmaceutic); 0 (Blood Glucose); 0 (Buffers); 0 (Delayed-Action Preparations); 0 (Drug Carriers); 0 (Insulin); 0 (Tablets); 9004-67-5 (Methylcellulose); 9NEZ333N27 (Sodium); G59NX3I3RT (Glycocholic Acid)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170924
[Lr] Data última revisão:
170924
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160814
[St] Status:MEDLINE


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[PMID]:27169745
[Au] Autor:Xu H; Zhang L; Kang H; Zhang J; Liu J; Liu S
[Ad] Endereço:Department of Clinical Laboratory, Tianjin Third Central Hospital, Tianjin, China.
[Ti] Título:Serum Metabonomics of Mild Acute Pancreatitis.
[So] Source:J Clin Lab Anal;30(6):990-998, 2016 Nov.
[Is] ISSN:1098-2825
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Mild acute pancreatitis (MAP) is a common acute abdominal disease, and exhibits rising incidence in recent decades. As an important component of systemic biology, metabonomics is a new discipline developed following genomics and proteomics. In this study, the objective was to analyze the serum metabonomics of patients with MAP, aiming to screen metabolic markers with potential diagnostic values. METHODS: An analysis platform with ultra performance liquid chromatography-high-resolution mass spectrometry was used to screen the difference metabolites related to MAP diagnosis and disease course monitoring. RESULTS: A total of 432 endogenous metabolites were screened out from 122 serum samples, and 49 difference metabolites were verified, among which 12 difference metabolites were identified by nonparametric test. After material identification, eight metabolites exhibited reliable results, and their levels in MAP serum were higher than those in healthy serum. Four metabolites exhibited gradual downward trend with treatment process going on, and the differences were statistically significant (P < 0.05). CONCLUSION: Metabonomic analysis has revealed eight metabolites with potential diagnostic values toward MAP, among which four metabolites can be used to monitor the disease course.
[Mh] Termos MeSH primário: Amilases/sangue
Decanoatos/sangue
Lipase/sangue
Metabolômica/métodos
Pancreatite/sangue
[Mh] Termos MeSH secundário: Doença Aguda
Adulto
Idoso
Cromatografia Líquida
Feminino
Ácido Glicocólico/sangue
Seres Humanos
Imagem por Ressonância Magnética
Masculino
Espectrometria de Massas
Meia-Idade
Pancreatite/diagnóstico por imagem
Análise de Componente Principal
Curva ROC
Esfingosina/análogos & derivados
Esfingosina/sangue
Máquina de Vetores de Suporte
Tironinas/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-tetradecanone); 0 (Decanoates); 0 (Thyronines); EC 3.1.1.3 (Lipase); EC 3.2.1.- (Amylases); G59NX3I3RT (Glycocholic Acid); NGZ37HRE42 (Sphingosine); OWA98U788S (safingol)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160513
[St] Status:MEDLINE
[do] DOI:10.1002/jcla.21969


  9 / 729 MEDLINE  
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[PMID]:27061917
[Au] Autor:Li QF; Zhan YM; Zhong YG; Zhang B; Ge CQ
[Ad] Endereço:Department of Hepatobiliary Surgery, the Second Hospital of Baoding, Baoding, Hebei Province, China.
[Ti] Título:Macromolecular Crowding Agents-Assisted Imprinted Polymers For Analysis Of Glycocholic Acid In Human Plasma And Urine.
[So] Source:Biomed Chromatogr;30(11):1706-1713, 2016 Nov.
[Is] ISSN:1099-0801
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Glycocholic acid (GCA) is a newly identified biomarker for hepatocellular carcinoma (HCC) patients. In this study, a method based on macromolecular crowding strategy has been applied for preparation of a molecularly imprinted polymer (MIP), which possesses high adsorption capacity for GCA. Polymethyl methacrylate was used as a macromolecular crowding agent, N-(3-aminopropyl)-methacrylamide hydrochloride as a functional monomer and ethylene dimethacrylate as a cross-linker. The morphology and binding characteristics of MIP were assessed by scanning electron microscopy and absorption experiments. The MIP was used as an adsorbent material to separate GCA, and the molecularly imprinted solid-phase extraction (MISPE) was carefully optimized. The MISPE combined with high-performance liquid chromatographic analysis was successfully used to determine the GCA in plasma and urine samples. When spiked levels ranged from 0.2 to 20 µmol L , the recoveries were between 94.3 and 100.5%. As a proof of principle, this proposed method has been validated on a small subset of HCC patients (n = 10) and healthy volunteers (n = 10). The average GCA concentrations of HCC patients in plasma and urine were about 25 and 2.8 times than that of healthy volunteers. Copyright © 2016 John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: Acrilamidas/química
Ácido Glicocólico/sangue
Ácido Glicocólico/urina
Impressão Molecular/métodos
Extração em Fase Sólida/métodos
[Mh] Termos MeSH secundário: Adsorção
Carcinoma Hepatocelular/sangue
Carcinoma Hepatocelular/urina
Cromatografia Líquida de Alta Pressão/métodos
Reagentes para Ligações Cruzadas/química
Ácido Glicocólico/análise
Seres Humanos
Limite de Detecção
Neoplasias Hepáticas/sangue
Neoplasias Hepáticas/urina
Metacrilatos/química
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acrylamides); 0 (Cross-Linking Reagents); 0 (Methacrylates); 7BK5G69305 (ethylene dimethacrylate); G59NX3I3RT (Glycocholic Acid)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170124
[Lr] Data última revisão:
170124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160411
[St] Status:MEDLINE
[do] DOI:10.1002/bmc.3737


  10 / 729 MEDLINE  
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[PMID]:26613247
[Au] Autor:Horváth G; Bencsura Á; Simon Á; Tochtrop GP; DeKoster GT; Covey DF; Cistola DP; Toke O
[Ad] Endereço:Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary.
[Ti] Título:Structural determinants of ligand binding in the ternary complex of human ileal bile acid binding protein with glycocholate and glycochenodeoxycholate obtained from solution NMR.
[So] Source:FEBS J;283(3):541-55, 2016 Feb.
[Is] ISSN:1742-4658
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: Besides aiding digestion, bile salts are important signal molecules exhibiting a regulatory role in metabolic processes. Human ileal bile acid binding protein (I-BABP) is an intracellular carrier of bile salts in the epithelial cells of the distal small intestine and has a key role in the enterohepatic circulation of bile salts. Positive binding cooperativity combined with site selectivity of glycocholate and glycochenodeoxycholate, the two most abundant bile salts in the human body, make human I-BABP a unique member of the family of intracellular lipid binding proteins. Solution NMR structure of the ternary complex of human I-BABP with glycocholate and glycochenodeoxycholate reveals an extensive network of hydrogen bonds and hydrophobic interactions stabilizing the bound bile salts. Conformational changes accompanying bile salt binding affects four major regions in the protein including the C/D, E/F and G/H loops as well as the helical segment. Most of these protein regions coincide with a previously described network of millisecond time scale fluctuations in the apo protein, a motion absent in the bound state. Comparison of the heterotypic doubly ligated complex with the unligated form provides further evidence of a conformation selection mechanism of ligand entry. Structural and dynamic aspects of human I-BABP-bile salt interaction are discussed and compared with characteristics of ligand binding in other members of the intracellular lipid binding protein family. PROTEIN DATA BANK ACCESSION NUMBERS: The coordinates of the 10 lowest energy structures of the human I-BABP : GCDA : GCA complex as well as the distance restraints used to calculate the final ensemble have been deposited in the Brookhaven Protein Data Bank with accession number 2MM3.
[Mh] Termos MeSH primário: Proteínas de Transporte/química
Ácido Glicoquenodesoxicólico/química
Ácido Glicocólico/química
Glicoproteínas de Membrana/química
[Mh] Termos MeSH secundário: Sítios de Ligação
Seres Humanos
Ligações de Hidrogênio
Interações Hidrofóbicas e Hidrofílicas
Ligantes
Espectroscopia de Ressonância Magnética
Estrutura Molecular
Soluções
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (Ligands); 0 (Membrane Glycoproteins); 0 (Solutions); 0 (bile acid binding proteins); 640-79-9 (Glycochenodeoxycholic Acid); G59NX3I3RT (Glycocholic Acid)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:160206
[Lr] Data última revisão:
160206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151128
[St] Status:MEDLINE
[do] DOI:10.1111/febs.13610



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