Base de dados : MEDLINE
Pesquisa : D04.210.500.155.580.130.500.236 [Categoria DeCS]
Referências encontradas : 2004 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 201 ir para página                         

  1 / 2004 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28977033
[Au] Autor:Zholobenko AV; Mouithys-Mickalad A; Dostal Z; Serteyn D; Modriansky M
[Ad] Endereço:Department of Medical Chemistry & Biochemistry, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.
[Ti] Título:On the causes and consequences of the uncoupler-like effects of quercetin and dehydrosilybin in H9c2 cells.
[So] Source:PLoS One;12(10):e0185691, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Quercetin and dehydrosilybin are polyphenols which are known to behave like uncouplers of respiration in isolated mitochondria. Here we investigated whether the effect is conserved in whole cells. Following short term incubation, neither compound uncouples mitochondrial respiration in whole H9c2 cells below 50µM. However, following hypoxia, or long term incubation, leak (state IV with oligomycin) oxygen consumption is increased by quercetin. Both compounds partially protected complex I respiration, but not complex II in H9c2 cells following hypoxia. In a permeabilised H9c2 cell model, the increase in leak respiration caused by quercetin is lowered by increased [ADP] and is increased by adenine nucleotide transporter inhibitor, atractyloside, but not bongkrekic acid. Both quercetin and dehydrosilybin dissipate mitochondrial membrane potential in whole cells. In the case of quercetin, the effect is potentiated post hypoxia. Genetically encoded Ca++ sensors, targeted to the mitochondria, enabled the use of fluorescence microscopy to show that quercetin decreased mitochondrial [Ca++] while dehydrosilybin did not. Likewise, quercetin decreases accumulation of [Ca++] in mitochondria following hypoxia. Fluorescent probes were used to show that both compounds decrease plasma membrane potential and increase cytosolic [Ca++]. We conclude that the uncoupler-like effects of these polyphenols are attenuated in whole cells compared to isolated mitochondria, but downstream effects are nevertheless apparent. Results suggest that the effect of quercetin observed in whole and permeabilised cells may originate in the mitochondria, while the mechanism of action of cardioprotection by dehydrosilybin may be less dependent on mitochondrial uncoupling than originally thought. Rather, protective effects may originate due to interactions at the plasma membrane.
[Mh] Termos MeSH primário: Quercetina/farmacologia
Silimarina/farmacologia
[Mh] Termos MeSH secundário: Animais
Cálcio/metabolismo
Linhagem Celular
Digitonina/farmacologia
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Microscopia Confocal
Microscopia de Fluorescência
Translocases Mitocondriais de ADP e ATP/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Silymarin); 0 (dehydrosilybin); 9068-80-8 (Mitochondrial ADP, ATP Translocases); 9IKM0I5T1E (Quercetin); KOO5CM684H (Digitonin); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171005
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185691


  2 / 2004 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28348078
[Au] Autor:Lin S; Voyton C; Morris MT; Ackroyd PC; Morris JC; Christensen KA
[Ad] Endereço:From the Departments of Chemistry and.
[Ti] Título:pH regulation in glycosomes of procyclic form .
[So] Source:J Biol Chem;292(19):7795-7805, 2017 May 12.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Here we report the use of a fluorescein-tagged peroxisomal targeting sequence peptide (F-PTS1, acetyl-C{K(FITC)}GGAKL) for investigating pH regulation of glycosomes in live procyclic form When added to cells, this fluorescent peptide is internalized within vesicular structures, including glycosomes, and can be visualized after 30-60 min. Using F-PTS1 we are able to observe the pH conditions inside glycosomes in response to starvation conditions. Previous studies have shown that in the absence of glucose, the glycosome exhibits mild acidification from pH 7.4 ± 0.2 to 6.8 ± 0.2. Our results suggest that this response occurs under proline starvation as well. This pH regulation is found to be independent from cytosolic pH and requires a source of Na ions. Glycosomes were also observed to be more resistant to external pH changes than the cytosol; placement of cells in acidic buffers (pH 5) reduced the pH of the cytosol by 0.8 ± 0.1 pH units, whereas glycosomal pH decreases by 0.5 ± 0.1 pH units. This observation suggests that regulation of glycosomal pH is different and independent from cytosolic pH regulation. Furthermore, pH regulation is likely to work by an active process, because cells depleted of ATP with 2-deoxyglucose and sodium azide were unable to properly regulate pH. Finally, inhibitor studies with bafilomycin and EIPA suggest that both V-ATPases and Na /H exchangers are required for glycosomal pH regulation.
[Mh] Termos MeSH primário: Microcorpos/química
Trypanosoma brucei brucei/química
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/química
Amilorida/análogos & derivados
Amilorida/química
Animais
Citosol/química
Desoxiglucose/química
Digitonina/química
Glucose/química
Homeostase
Concentração de Íons de Hidrogênio
Macrolídeos/química
Microscopia de Fluorescência
Potássio/química
Prolina/química
Domínios Proteicos
Proteínas de Protozoários/química
Azida Sódica/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Macrolides); 0 (Protozoan Proteins); 116764-51-3 (bafilomycin A); 7DZO8EB0Z3 (Amiloride); 8L70Q75FXE (Adenosine Triphosphate); 968JJ8C9DV (Sodium Azide); 9DLQ4CIU6V (Proline); 9G2MP84A8W (Deoxyglucose); IY9XDZ35W2 (Glucose); KOO5CM684H (Digitonin); RWP5GA015D (Potassium); VW50CE070T (ethylisopropylamiloride)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170329
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.784173


  3 / 2004 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27603739
[Au] Autor:Giner JL; Feng J; Kiemle DJ
[Ad] Endereço:Department of Chemistry, State University of New York-ESF , Syracuse, New York 13210, United States.
[Ti] Título:NMR Tube Degradation Method for Sugar Analysis of Glycosides.
[So] Source:J Nat Prod;79(9):2413-7, 2016 Sep 23.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The sugar subunits of natural glycosides can be conveniently determined by acid hydrolysis and (1)H NMR spectroscopy without isolation or derivatization. The chemical shifts, coupling constants, and integral ratios of the anomeric signals allow each monosaccharide to be identified and its molar ratio to other monosaccharides to be quantified. The NMR data for the anomeric signals of 28 monosaccharides and three disaccharides are reported. Application of the method is demonstrated with the flavonoid glycoside naringin (1), the aminoglycoside antibiotics kanamycin (2) and tobramycin (3), and the saponin digitonin (4).
[Mh] Termos MeSH primário: Glicosídeos/análise
Ressonância Magnética Nuclear Biomolecular/métodos
[Mh] Termos MeSH secundário: Digitonina/química
Dissacarídeos/química
Flavanonas/química
Glicosídeos/química
Canamicina/química
Estrutura Molecular
Monossacarídeos/química
Tobramicina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Disaccharides); 0 (Flavanones); 0 (Glycosides); 0 (Monosaccharides); 59-01-8 (Kanamycin); KOO5CM684H (Digitonin); N7TD9J649B (naringin); VZ8RRZ51VK (Tobramycin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170627
[Lr] Data última revisão:
170627
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160908
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.6b00180


  4 / 2004 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:27602576
[Au] Autor:Wheeler JR; Matheny T; Jain S; Abrisch R; Parker R
[Ad] Endereço:Department of Chemistry and Biochemistry, University of Colorado Boulder, Boulder, United States.
[Ti] Título:Distinct stages in stress granule assembly and disassembly.
[So] Source:Elife;5, 2016 Sep 07.
[Is] ISSN:2050-084X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Stress granules are non-membrane bound RNA-protein (RNP) assemblies that form when translation initiation is limited and contain a biphasic structure with stable core structures surrounded by a less concentrated shell. The order of assembly and disassembly of these two structures remains unknown. Time course analysis of granule assembly suggests that core formation is an early event in granule assembly. Stress granule disassembly is also a stepwise process with shell dissipation followed by core clearance. Perturbations that alter liquid-liquid phase separations (LLPS) driven by intrinsically disordered protein regions (IDR) of RNA binding proteins in vitro have the opposite effect on stress granule assembly in vivo. Taken together, these observations argue that stress granules assemble through a multistep process initiated by stable assembly of untranslated mRNPs into core structures, which could provide sufficient high local concentrations to allow for a localized LLPS driven by IDRs on RNA binding proteins.
[Mh] Termos MeSH primário: Grânulos Citoplasmáticos/metabolismo
Proteínas Intrinsicamente Desordenadas/genética
RNA Mensageiro/genética
Ribonucleoproteínas/genética
Saccharomyces cerevisiae/genética
[Mh] Termos MeSH secundário: Arsenitos/farmacologia
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Cicloeximida/farmacologia
Grânulos Citoplasmáticos/efeitos dos fármacos
Grânulos Citoplasmáticos/ultraestrutura
Digitonina/farmacologia
Glicóis/farmacologia
Células HeLa
Seres Humanos
Proteínas Intrinsicamente Desordenadas/metabolismo
Iniciação Traducional da Cadeia Peptídica/efeitos dos fármacos
RNA Mensageiro/metabolismo
Ribonucleoproteínas/metabolismo
Saccharomyces cerevisiae/efeitos dos fármacos
Saccharomyces cerevisiae/metabolismo
Saccharomyces cerevisiae/ultraestrutura
Compostos de Sódio/farmacologia
Estresse Fisiológico
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arsenites); 0 (Glycols); 0 (Intrinsically Disordered Proteins); 0 (RNA, Messenger); 0 (Ribonucleoproteins); 0 (Sodium Compounds); 0 (messenger ribonucleoprotein); 48OVY2OC72 (sodium arsenite); 98600C0908 (Cycloheximide); KOO5CM684H (Digitonin); ZIA319275I (hexamethylene glycol)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170613
[Lr] Data última revisão:
170613
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160908
[St] Status:MEDLINE


  5 / 2004 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27284005
[Au] Autor:Au S; Wu W; Zhou L; Theilmann DA; Panté N
[Ad] Endereço:Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada.
[Ti] Título:A new mechanism for nuclear import by actin-based propulsion used by a baculovirus nucleocapsid.
[So] Source:J Cell Sci;129(15):2905-11, 2016 Aug 01.
[Is] ISSN:1477-9137
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The transport of macromolecules into the nucleus is mediated by soluble cellular receptors of the importin ß superfamily and requires the Ran-GTPase cycle. Several studies have provided evidence that there are exceptions to this canonical nuclear import pathway. Here, we report a new unconventional nuclear import mechanism exploited by the baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV). We found that AcMNPV nucleocapsids entered the nucleus of digitonin-permeabilized cells in the absence of exogenous cytosol or under conditions that blocked the Ran-GTPase cycle. AcMNPV contains a protein that activates the Arp2/3 complex and induces actin polymerization at one end of the rod-shaped nucleocapsid. We show that inhibitors of Arp2/3 blocked nuclear import of nucleocapsids in semi-permeabilized cells. Nuclear import of nucleocapsids was also reconstituted in purified nuclei supplemented with G-actin and Arp2/3 under actin polymerization conditions. Thus, we propose that actin polymerization drives not only migration of baculovirus through the cytoplasm but also pushes the nucleocapsid through the nuclear pore complex to enter the cell nucleus. Our findings point to a very distinct role of actin-based motility during the baculovirus infection cycle.
[Mh] Termos MeSH primário: Actinas/metabolismo
Baculoviridae/metabolismo
Núcleo Celular/metabolismo
Nucleocapsídeo/metabolismo
[Mh] Termos MeSH secundário: Complexo 2-3 de Proteínas Relacionadas à Actina/metabolismo
Transporte Ativo do Núcleo Celular/efeitos dos fármacos
Baculoviridae/efeitos dos fármacos
Permeabilidade da Membrana Celular/efeitos dos fármacos
Citosol/efeitos dos fármacos
Citosol/metabolismo
Digitonina/farmacologia
Guanosina 5'-O-(3-Tiotrifosfato)/metabolismo
Células HeLa
Seres Humanos
Poro Nuclear/metabolismo
Nucleocapsídeo/efeitos dos fármacos
Nucleopolyhedrovirus/efeitos dos fármacos
Nucleopolyhedrovirus/metabolismo
Polimerização/efeitos dos fármacos
Quinazolinas/farmacologia
Proteína ran de Ligação ao GTP/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actin-Related Protein 2-3 Complex); 0 (Actins); 0 (Quinazolines); 0 (importazole); 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate)); EC 3.6.5.2 (ran GTP-Binding Protein); KOO5CM684H (Digitonin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160611
[St] Status:MEDLINE
[do] DOI:10.1242/jcs.191668


  6 / 2004 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26968775
[Au] Autor:Rivero LA; Concepción JL; Quintero-Troconis E; Quiñones W; Michels PA; Acosta H
[Ad] Endereço:Laboratorio de Enzimología de Parásitos, Facultad de Ciencias, Universidad de Los Andes, Mérida 5101, Venezuela.
[Ti] Título:Trypanosoma evansi contains two auxiliary enzymes of glycolytic metabolism: Phosphoenolpyruvate carboxykinase and pyruvate phosphate dikinase.
[So] Source:Exp Parasitol;165:7-15, 2016 Jun.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Trypanosoma evansi is a monomorphic protist that can infect horses and other animal species of economic importance for man. Like the bloodstream form of the closely related species Trypanosoma brucei, T. evansi depends exclusively on glycolysis for its free-energy generation. In T. evansi as in other kinetoplastid organisms, the enzymes of the major part of the glycolytic pathway are present within organelles called glycosomes, which are authentic but specialized peroxisomes. Since T. evansi does not undergo stage-dependent differentiations, it occurs only as bloodstream forms, it has been assumed that the metabolic pattern of this parasite is identical to that of the bloodstream form of T. brucei. However, we report here the presence of two additional enzymes, phosphoenolpyruvate carboxykinase and PPi-dependent pyruvate phosphate dikinase in T. evansi glycosomes. Their colocalization with glycolytic enzymes within the glycosomes of this parasite has not been reported before. Both enzymes can make use of PEP for contributing to the production of ATP within the organelles. The activity of these enzymes in T. evansi glycosomes drastically changes the model assumed for the oxidation of glucose by this parasite.
[Mh] Termos MeSH primário: Fosfoenolpiruvato Carboxiquinase (ATP)/metabolismo
Piruvato Ortofosfato Diquinase/metabolismo
Trypanosoma/enzimologia
[Mh] Termos MeSH secundário: Animais
Digitonina/farmacologia
Glucosefosfato Desidrogenase/isolamento & purificação
Glucosefosfato Desidrogenase/metabolismo
Glicólise
Hexoquinase/isolamento & purificação
Hexoquinase/metabolismo
Cavalos
Indicadores e Reagentes/farmacologia
Malato Desidrogenase/isolamento & purificação
Malato Desidrogenase/metabolismo
Camundongos
Microcorpos/enzimologia
Microscopia de Fluorescência
Permeabilidade/efeitos dos fármacos
Fosfoenolpiruvato Carboxiquinase (ATP)/genética
Fosfoenolpiruvato Carboxiquinase (ATP)/isolamento & purificação
Fosfoglicerato Quinase/isolamento & purificação
Fosfoglicerato Quinase/metabolismo
Fosfopiruvato Hidratase/isolamento & purificação
Fosfopiruvato Hidratase/metabolismo
Piruvato Ortofosfato Diquinase/isolamento & purificação
Coelhos
Ratos
Ratos Wistar
Trypanosoma/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indicators and Reagents); EC 1.1.1.37 (Malate Dehydrogenase); EC 1.1.1.49 (Glucosephosphate Dehydrogenase); EC 2.7.1.1 (Hexokinase); EC 2.7.2.3 (Phosphoglycerate Kinase); EC 2.7.9.1 (Pyruvate, Orthophosphate Dikinase); EC 4.1.1.49 (Phosphoenolpyruvate Carboxykinase (ATP)); EC 4.2.1.11 (Phosphopyruvate Hydratase); KOO5CM684H (Digitonin)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170118
[Lr] Data última revisão:
170118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160313
[St] Status:MEDLINE


  7 / 2004 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:26785823
[Au] Autor:Jäger F; Zoltner M; Kneuper H; Hunter WN; Palmer T
[Ad] Endereço:Divisions of Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee, UK.
[Ti] Título:Membrane interactions and self-association of components of the Ess/Type VII secretion system of Staphylococcus aureus.
[So] Source:FEBS Lett;590(3):349-57, 2016 Feb.
[Is] ISSN:1873-3468
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The Ess/Type VII protein secretion system, essential for virulence of pathogenic Staphylococcus aureus, is dependent upon the four core membrane proteins EssA, EssB, EssC and EsaA. Here, we use crosslinking and blue native PAGE analysis to show that the EssB, EssC and EsaA proteins individually form homomeric complexes. Surprisingly, these components appear unable to interact with each other, or with the EssA protein. We further show that two high molecular weight multimers of EssC detected in whole cells are not dependent upon the presence of EsxA, EsxB or any other Ess component for their assembly.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Modelos Biológicos
Staphylococcus aureus/metabolismo
Sistemas de Secreção Tipo VII/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Reagentes para Ligações Cruzadas/química
Detergentes/química
Digitonina/química
Dimerização
Formaldeído/química
Deleção de Genes
Glucosídeos/química
Peso Molecular
Eletroforese em Gel de Poliacrilamida Nativa
Octoxinol/química
Fases de Leitura Aberta
Fosforilcolina/análogos & derivados
Fosforilcolina/química
Polímeros/química
Proteínas Recombinantes de Fusão/química
Proteínas Recombinantes de Fusão/metabolismo
Proteínas Recombinantes/química
Proteínas Recombinantes/metabolismo
Solubilidade
Succinimidas/química
Sistemas de Secreção Tipo VII/química
Sistemas de Secreção Tipo VII/genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Cross-Linking Reagents); 0 (Detergents); 0 (Glucosides); 0 (Polymers); 0 (Recombinant Fusion Proteins); 0 (Recombinant Proteins); 0 (Succinimides); 0 (Type VII Secretion Systems); 107-73-3 (Phosphorylcholine); 1HG84L3525 (Formaldehyde); 53949-18-1 (dodecylphosphocholine); 69227-93-6 (dodecyl maltoside); 9002-93-1 (Octoxynol); KOO5CM684H (Digitonin); V9WYZ7QMDT (disuccinimidyl suberate); Y19UC83H8E (paraform)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160121
[St] Status:MEDLINE
[do] DOI:10.1002/1873-3468.12065


  8 / 2004 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:26698328
[Au] Autor:Shinzawa-Itoh K; Shimomura H; Yanagisawa S; Shimada S; Takahashi R; Oosaki M; Ogura T; Tsukihara T
[Ad] Endereço:From the Department of Life Science, Graduate School of Life Science, University of Hyogo, 3-2-1, Koto, Kamighori, Akoh, Hyogo, 678-1297, Japan, shinzawa@sci.u-hyogo.ac.jp.
[Ti] Título:Purification of Active Respiratory Supercomplex from Bovine Heart Mitochondria Enables Functional Studies.
[So] Source:J Biol Chem;291(8):4178-84, 2016 Feb 19.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To understand the roles of mitochondrial respiratory chain supercomplexes, methods for consistently separating and preparing supercomplexes must be established. To this end, we solubilized supercomplexes from bovine heart mitochondria with digitonin and then replaced digitonin with amphipol (A8-35), an amphiphilic polymer. Afterward, supercomplexes were separated from other complexes by sucrose density gradient centrifugation. Twenty-six grams of bovine myocardium yielded 3.2 mg of amphipol-stabilized supercomplex. The purified supercomplexes were analyzed based on their absorption spectra as well as Q10 (ubiquinone with ten isoprene units) and lipid assays. The supercomplex sample did not contain cytochrome c but did contain complexes I, III, and IV at a ratio of 1:2:1, 6 molecules of Q10, and 623 atoms of phosphorus. When cytochrome c was added, the supercomplex exhibited KCN-sensitive NADH oxidation; thus, the purified supercomplex was active. Reduced complex IV absorbs at 444 nm, so we measured the resonance Raman spectrum of the reduced amphipol-solubilized supercomplex and the mixture of amphipol-solubilized complexes I1, III2, and IV1 using an excitation wavelength of 441.6 nm, allowing measurement precision comparable with that obtained for complex IV alone. Use of the purified active sample provides insights into the effects of supercomplex formation.
[Mh] Termos MeSH primário: Digitonina/química
Complexo de Proteínas da Cadeia de Transporte de Elétrons/isolamento & purificação
Mitocôndrias Cardíacas/enzimologia
Proteínas Musculares/isolamento & purificação
Miocárdio/enzimologia
Polímeros/química
Propilaminas/química
[Mh] Termos MeSH secundário: Animais
Bovinos
Complexo de Proteínas da Cadeia de Transporte de Elétrons/química
Proteínas Musculares/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Electron Transport Chain Complex Proteins); 0 (Muscle Proteins); 0 (Polymers); 0 (Propylamines); 0 (amphipol A8-35); KOO5CM684H (Digitonin)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:160224
[Lr] Data última revisão:
160224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151225
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M115.680553


  9 / 2004 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26564236
[Au] Autor:Mito M; Kawaguchi T; Hirose T; Nakagawa S
[Ad] Endereço:RNA Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako 351-0198, Japan.
[Ti] Título:Simultaneous multicolor detection of RNA and proteins using super-resolution microscopy.
[So] Source:Methods;98:158-165, 2016 Apr 01.
[Is] ISSN:1095-9130
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A number of non-membranous cellular bodies have been identified in higher eukaryotes, and these bodies contain a specific set of proteins and RNAs that are used to fulfill their functions. The size of these RNA-containing cellular bodies is usually on a submicron scale, making it difficult to observe fine structures using optical microscopy due to the diffraction limitation of visible light. Recently, microscope companies have released super-resolution microscopes that were developed using different principles, enabling the observation of sub-micron structures not resolvable in conventional fluorescent microscopy. Here, we describe multi-color fluorescent in situ hybridization techniques optimized for the simultaneous detection of RNA and proteins using super-resolution microscopy, namely structured illumination microscopy (SIM).
[Mh] Termos MeSH primário: Hibridização in Situ Fluorescente/métodos
Microscopia de Fluorescência/métodos
Sondas RNA/química
RNA Longo não Codificante/análise
Transcrição Genética
[Mh] Termos MeSH secundário: Anticorpos/química
Biotina/química
Linhagem Celular
DNA Helicases/análise
DNA Helicases/genética
DNA Helicases/metabolismo
Digitonina/química
Fluoresceína-5-Isotiocianato/química
Haptenos/química
Seres Humanos
Imagem Tridimensional
Proteínas Associadas à Matriz Nuclear/análise
Proteínas Associadas à Matriz Nuclear/genética
Proteínas Associadas à Matriz Nuclear/metabolismo
Proteínas Nucleares/análise
Proteínas Nucleares/genética
Proteínas Nucleares/metabolismo
Fatores de Transcrição de Octâmero/análise
Fatores de Transcrição de Octâmero/genética
Fatores de Transcrição de Octâmero/metabolismo
Fator de Processamento Associado a PTB/análise
Fator de Processamento Associado a PTB/genética
Fator de Processamento Associado a PTB/metabolismo
RNA Longo não Codificante/genética
RNA Longo não Codificante/metabolismo
Proteína FUS de Ligação a RNA/análise
Proteína FUS de Ligação a RNA/genética
Proteína FUS de Ligação a RNA/metabolismo
Proteínas de Ligação a RNA/análise
Proteínas de Ligação a RNA/genética
Proteínas de Ligação a RNA/metabolismo
Coloração e Rotulagem/métodos
Fatores de Transcrição/análise
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies); 0 (FUS protein, human); 0 (Haptens); 0 (NEAT1 long non-coding RNA, human); 0 (NONO protein, human); 0 (Nuclear Matrix-Associated Proteins); 0 (Nuclear Proteins); 0 (Octamer Transcription Factors); 0 (PTB-Associated Splicing Factor); 0 (RNA Probes); 0 (RNA, Long Noncoding); 0 (RNA-Binding Protein FUS); 0 (RNA-Binding Proteins); 0 (Transcription Factors); 6SO6U10H04 (Biotin); EC 3.6.1.- (SMARCA4 protein, human); EC 3.6.4.- (DNA Helicases); I223NX31W9 (Fluorescein-5-isothiocyanate); KOO5CM684H (Digitonin)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170722
[Lr] Data última revisão:
170722
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151114
[St] Status:MEDLINE


  10 / 2004 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26518518
[Au] Autor:Berger ML
[Ad] Endereço:Center for Brain Research, Medical University of Vienna, A-1090 Vienna, Austria.
[Ti] Título:Soaping the NMDA receptor: Various types of detergents influence differently [(3)H]MK-801 binding to rat brain membranes.
[So] Source:Biochim Biophys Acta;1858(1):116-22, 2016 Jan.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Membranes prepared from rat brain were treated with increasing concentrations of cationic, neutral, anionic and zwitterionic surfactants. Potent inactivation of [(3)H]MK-801 binding to NMDA receptors (NRs) was provided by the cation cetyl pyridinium (IC50 25 µM) and the neutral digitonin (IC50 37 µM). A 2 h incubation of rat brain membranes at 24°C with 100 µM of the neutral Triton X-100 resulted in about 50% reversible inhibition (without inactivation). Reversible inhibition was also effected by the anion deoxycholate (IC50 700 µM), and by the zwitterions N-lauryl sulfobetaine (12-SB(±), 400 µM) and CHAPS (1.5 mM), with inactivation at higher concentrations. Keeping the NR cation channel in the closed state significantly protected against inactivation by cations and by 12-SB(±), but not by the other detergents. Inactivation depended differentially on the amount of the membranes, on the duration of the treatment, and on the temperature. Varying the amount of membranes by a factor 8 yielded for cetyl trimethylammonium (16-NMe3(+)) IC50s of inactivation from 10 to 80 µM, while for deoxycholate the IC50 of inactivation was 1.2 mM for all tissue quantities. Some compounds inactivated within a few min (16-NMe3(+), digitonin, CHAPS), while inactivation by others took at least half an hour (Triton X-100, deoxycholate, 12-SB(±)). These last 3 ones also exhibited the steepest temperature dependence. Knowledge about the influence of various parameters is helpful in selecting appropriate conditions allowing the treatment of brain membranes with amphiphiles without risking irreversible inactivation.
[Mh] Termos MeSH primário: Membrana Celular/efeitos dos fármacos
Detergentes/química
Maleato de Dizocilpina/química
Antagonistas de Aminoácidos Excitatórios/química
Receptores de N-Metil-D-Aspartato/química
[Mh] Termos MeSH secundário: Animais
Membrana Celular/química
Córtex Cerebral/química
Compostos de Cetrimônio/química
Compostos de Cetrimônio/farmacologia
Ácidos Cólicos/química
Ácidos Cólicos/farmacologia
Ácido Desoxicólico/química
Ácido Desoxicólico/farmacologia
Detergentes/farmacologia
Digitonina/química
Digitonina/farmacologia
Maleato de Dizocilpina/farmacologia
Antagonistas de Aminoácidos Excitatórios/farmacologia
Hipocampo/química
Masculino
Octoxinol/química
Octoxinol/farmacologia
Compostos de Amônio Quaternário/química
Compostos de Amônio Quaternário/farmacologia
Ratos
Ratos Wistar
Receptores de N-Metil-D-Aspartato/antagonistas & inibidores
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cetrimonium Compounds); 0 (Cholic Acids); 0 (Detergents); 0 (Excitatory Amino Acid Antagonists); 0 (Quaternary Ammonium Compounds); 0 (Receptors, N-Methyl-D-Aspartate); 005990WHZZ (Deoxycholic Acid); 14933-08-5 (zwittergent 3-12); 6LR8C1B66Q (Dizocilpine Maleate); 9002-93-1 (Octoxynol); KOO5CM684H (Digitonin); QBP25342AG (3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate); Z7FF1XKL7A (cetrimonium)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151101
[St] Status:MEDLINE



página 1 de 201 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde