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Pesquisa : D04.210.500.247 [Categoria DeCS]
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[PMID]:28899902
[Au] Autor:Thompson D; Morrice N; Grant L; Le Sommer S; Lees EK; Mody N; Wilson HM; Delibegovic M
[Ad] Endereço:School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen, U.K. m.delibegovic@abdn.ac.uk dthompson@abdn.ac.uk.
[Ti] Título:Pharmacological inhibition of protein tyrosine phosphatase 1B protects against atherosclerotic plaque formation in the LDLR mouse model of atherosclerosis.
[So] Source:Clin Sci (Lond);131(20):2489-2501, 2017 Oct 01.
[Is] ISSN:1470-8736
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cardiovascular disease (CVD) is the most prevalent cause of mortality among patients with type 1 or type 2 diabetes, due to accelerated atherosclerosis. Recent evidence suggests a strong link between atherosclerosis and insulin resistance, due to impaired insulin receptor (IR) signalling. Here, we demonstrate that inhibiting the activity of protein tyrosine phosphatase 1B (PTP1B), the major negative regulator of the IR prevents and reverses atherosclerotic plaque formation in an LDLR mouse model of atherosclerosis. Acute (single dose) or chronic PTP1B inhibitor (trodusquemine) treatment of LDLR mice decreased weight gain and adiposity, improved glucose homeostasis and attenuated atherosclerotic plaque formation. This was accompanied by a reduction in both, circulating total cholesterol and triglycerides, a decrease in aortic monocyte chemoattractant protein-1 (MCP-1) expression levels and hyperphosphorylation of aortic Akt/PKB and AMPKα. Our findings are the first to demonstrate that PTP1B inhibitors could be used in prevention and reversal of atherosclerosis development and reduction in CVD risk.
[Mh] Termos MeSH primário: Aorta/efeitos dos fármacos
Doenças da Aorta/prevenção & controle
Aterosclerose/prevenção & controle
Colestanos/administração & dosagem
Inibidores Enzimáticos/administração & dosagem
Placa Aterosclerótica
Proteína Tirosina Fosfatase não Receptora Tipo 1/antagonistas & inibidores
Receptores de LDL/deficiência
Espermina/análogos & derivados
[Mh] Termos MeSH secundário: Proteínas Quinases Ativadas por AMP/metabolismo
Animais
Aorta/enzimologia
Aorta/patologia
Doenças da Aorta/enzimologia
Doenças da Aorta/genética
Doenças da Aorta/patologia
Aterosclerose/enzimologia
Aterosclerose/genética
Aterosclerose/patologia
Biomarcadores/sangue
Glicemia/efeitos dos fármacos
Glicemia/metabolismo
Quimiocina CCL2/metabolismo
Colesterol/sangue
Dieta Hiperlipídica
Modelos Animais de Doenças
Esquema de Medicação
Predisposição Genética para Doença
Homeostase
Masculino
Camundongos Knockout
Fenótipo
Fosforilação
Proteína Tirosina Fosfatase não Receptora Tipo 1/metabolismo
Proteínas Proto-Oncogênicas c-akt/metabolismo
Receptores de LDL/genética
Transdução de Sinais/efeitos dos fármacos
Espermina/administração & dosagem
Fatores de Tempo
Triglicerídeos/sangue
Perda de Peso
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3-N-1(spermine)-7, 24-dihydroxy-5-cholestane 24-sulfate); 0 (Biomarkers); 0 (Blood Glucose); 0 (Ccl2 protein, mouse); 0 (Chemokine CCL2); 0 (Cholestanes); 0 (Enzyme Inhibitors); 0 (Receptors, LDL); 0 (Triglycerides); 2FZ7Y3VOQX (Spermine); 97C5T2UQ7J (Cholesterol); EC 2.7.11.1 (AMPK alpha1 subunit, mouse); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt); EC 2.7.11.31 (AMP-Activated Protein Kinases); EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 1); EC 3.1.3.48 (Ptpn1 protein, mouse)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE
[do] DOI:10.1042/CS20171066


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[PMID]:28139298
[Au] Autor:Wang L; Jiang XL; Zhang WM; Li F; Khan AA; Liu X; Yu K; Wang MK
[Ad] Endereço:School of Pharmacy and Bioengineering, Chengdu University, Chengdu, 610106, China; Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of S
[Ti] Título:Homo-aro-cholestane, furostane and spirostane saponins from the tubers of Ophiopogon japonicus.
[So] Source:Phytochemistry;136:125-132, 2017 Apr.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phytochemical investigation of the tubers of Ophiopogon japonicus led to the isolation of five previously undescribed steroidal saponins, ophiojaponins A-E, together with twelve known ones. The structures of these isolated compounds were elucidated by detailed spectroscopic analyses and chemical methods. Ophiojaponins A-C are rare naturally occurring C steroidal glycosides possessing a homo-cholestane skeleton with an aromatized ring E. Ruscogenin 1-O-α-L-rhamnopyranosyl-(1 â†’ 2)-4-O-sulfo-ß-D-fucopyranosido-3-O-ß-D-glucopyranoside was isolated as single component and its full spectroscopic data was reported for the first time. The isolated steroidal saponins were evaluated for their cytotoxicities against two human tumor cell lines MG-63 and SNU387. Among them, five known spirostane-type glycosides showed cytotoxic activity against both MG-63 and SNU387 cell lines with IC values ranging from 0.76 to 27.0 µM.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/isolamento & purificação
Colestanos/isolamento & purificação
Medicamentos de Ervas Chinesas/isolamento & purificação
Ophiopogon/química
Tubérculos/química
Saponinas/isolamento & purificação
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/farmacologia
Colestanos/química
Colestanos/farmacologia
Ensaios de Seleção de Medicamentos Antitumorais
Medicamentos de Ervas Chinesas/química
Medicamentos de Ervas Chinesas/farmacologia
Estrutura Molecular
Saponinas/química
Saponinas/farmacologia
Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Cholestanes); 0 (Drugs, Chinese Herbal); 0 (Saponins)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170321
[Lr] Data última revisão:
170321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170201
[St] Status:MEDLINE


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[PMID]:27343991
[Au] Autor:Dalenc F; Iuliano L; Filleron T; Zerbinati C; Voisin M; Arellano C; Chatelut E; Marquet P; Samadi M; Roché H; Poirot M; Silvente-Poirot S
[Ad] Endereço:Department of Medical Oncology, Institut Claudius Regaud, IUCT-Oncopole, Toulouse, France; Inserm UMR 1037, Team "Cholesterol metabolism and therapeutic innovations", Cancer Research Center of Toulouse, Toulouse, France. Electronic address: dalenc.florence@iuct-oncople.fr.
[Ti] Título:Circulating oxysterol metabolites as potential new surrogate markers in patients with hormone receptor-positive breast cancer: Results of the OXYTAM study.
[So] Source:J Steroid Biochem Mol Biol;169:210-218, 2017 May.
[Is] ISSN:1879-1220
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Accumulating evidence indicates that cholesterol oxygenation products, also known as oxysterols (OS), are involved in breast cancer (BC) promotion. The impact of Tam, as well as aromatase inhibitors (AI), an alternative BC endocrine therapy (ET), on OS metabolism in patients is currently unknown. We conducted a prospective clinical study in BC patients receiving Tam (n=15) or AI (n=14) in adjuvant or in metastatic settings. The primary end point was the feasibility of detecting and quantifying 11 different OS in the circulation of patients before and after 28days of treatment with Tam or AI. Key secondary end points were the measurements of variations in the concentrations of OS according to differences between patients and treatments. OS profiling in the serum of patients was determined by gas chromatography coupled to mass spectrometry. OS profiling was conducted in all patients both at baseline and during treatment regimens. An important inter-individual variability was observed for each OS. Interestingly 5,6ß-epoxycholesterol relative concentrations significantly increased in the entire population (p=0.0109), while no increase in Cholestane-triol (CT) levels was measured. Interestingly, we found that, in contrast to AI, Tam therapy significantly decreased blood levels of 24-hydroxycholesterol (24-HC), 7α-HC and 25-HC (a tumor promoter) (p=0.0007, p=0.0231 and p=0.0231, respectively), whereas 4ß-HC levels increased (p=0.0010). Interestingly, levels of 27-HC (a tumor promoter) significantly increased in response to AI (p=0.0342), but not Tam treatment. According to these results, specific OS are promising candidate markers of Tam and AI efficacy. Thus, further clinical investigations are needed to confirm the use of oxysterols as biomarkers of both prognosis and/or the efficacy of ET.
[Mh] Termos MeSH primário: Neoplasias da Mama/sangue
Oxisteróis/metabolismo
[Mh] Termos MeSH secundário: Adulto
Idoso
Androstadienos/uso terapêutico
Aromatase/metabolismo
Inibidores da Aromatase/uso terapêutico
Biomarcadores/sangue
Índice de Massa Corporal
Neoplasias da Mama/metabolismo
Colestanos/sangue
Colesterol/análogos & derivados
Colesterol/metabolismo
Estudos de Viabilidade
Feminino
Cromatografia Gasosa-Espectrometria de Massas
Hormônios/química
Seres Humanos
Meia-Idade
Metástase Neoplásica
Nitrilos/uso terapêutico
Estresse Oxidativo
Oxisteróis/sangue
Projetos Piloto
Prognóstico
Estudos Prospectivos
Reprodutibilidade dos Testes
Transdução de Sinais
Tamoxifeno/uso terapêutico
Triazóis/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (5,6-epoxycholesterol); 0 (Androstadienes); 0 (Aromatase Inhibitors); 0 (Biomarkers); 0 (Cholestanes); 0 (Hormones); 0 (Nitriles); 0 (Oxysterols); 0 (Triazoles); 094ZI81Y45 (Tamoxifen); 7LKK855W8I (letrozole); 97C5T2UQ7J (Cholesterol); EC 1.14.14.1 (Aromatase); NY22HMQ4BX (exemestane)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160626
[St] Status:MEDLINE


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Bydlowski, Sergio Paulo
Texto completo
[PMID]:27133385
[Au] Autor:Silva SF; Levy D; Ruiz JLM; de Melo TC; Isaac C; Fidelis ML; Rodrigues A; Bydlowski SP
[Ad] Endereço:Laboratory of Genetics and Molecular Hematology (LIM31), University of São Paulo School of Medicine, Av.Dr.Enéas de Carvalho Aguiar, 155, 1st floor, room 43, 05403-000, São Paulo/SP, Brazil.
[Ti] Título:Oxysterols in adipose tissue-derived mesenchymal stem cell proliferation and death.
[So] Source:J Steroid Biochem Mol Biol;169:164-175, 2017 May.
[Is] ISSN:1879-1220
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Mesenchymal stem cells (MSCs) are multipotent cells characterized by self-renewal and cellular differentiation capabilities. Oxysterols comprise a very heterogeneous group derived from cholesterol through enzymatic and non-enzymatic oxidation. Potent effects in cell death processes, including cytoxicity and apoptosis induction, were described in several cell lines. Very little is known about the effects of oxysterols in MSCs. 7-ketocholesterol (7-KC), one of the most important oxysterols, was shown to be cytotoxic to human adipose tissue-derived MSCs. Here, we describe the short-term (24h) cytotoxic effects of cholestan-3α-5ß-6α-triol, 3,5 cholestan-7-one, (3α-5ß-6α)- cholestane-3,6-diol, 7-oxocholest-5-en-3ß-yl acetate, and 5ß-6ß epoxy-cholesterol, on MSCs derived from human adipose tissue. MSCs were isolated from adipose tissue obtained from three young, healthy women. Oxysterols, with the exception of 3,5 cholestan-7-one and 7-oxocholest-5-en-3ß-yl acetate, led to a complex mode of cell death that include apoptosis, necrosis and autophagy, depending on the type of oxysterol and concentration, being cholestan-3α-5ß-6α-triol the most effective. Inhibition of proliferation was also promoted by these oxysterols, but no changes in cell cycle were observed.
[Mh] Termos MeSH primário: Tecido Adiposo/citologia
Células Mesenquimais Estromais/citologia
Células Mesenquimais Estromais/efeitos dos fármacos
Oxisteróis/farmacologia
[Mh] Termos MeSH secundário: Actinas/metabolismo
Adulto
Apoptose
Autofagia
Caspase 3/metabolismo
Caspase 7/metabolismo
Ciclo Celular
Proliferação Celular
Sobrevivência Celular
Colestanos/farmacologia
Feminino
Técnica Indireta de Fluorescência para Anticorpo
Seres Humanos
Cetocolesteróis/farmacologia
Potenciais da Membrana
Meia-Idade
Mitocôndrias/metabolismo
Necrose
Oxirredução
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins); 0 (Cholestanes); 0 (Ketocholesterols); 0 (Oxysterols); EC 3.4.22.- (CASP3 protein, human); EC 3.4.22.- (CASP7 protein, human); EC 3.4.22.- (Caspase 3); EC 3.4.22.- (Caspase 7); O7676FE78M (7-ketocholesterol)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160503
[St] Status:MEDLINE


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[PMID]:27102612
[Au] Autor:Kasimov MR; Zakyrjanova GF; Giniatullin AR; Zefirov AL; Petrov AM
[Ad] Endereço:Department of Normal Physiology, Kazan State Medical University, Kazan 420012, Russia.
[Ti] Título:Similar oxysterols may lead to opposite effects on synaptic transmission: Olesoxime versus 5α-cholestan-3-one at the frog neuromuscular junction.
[So] Source:Biochim Biophys Acta;1861(7):606-16, 2016 Jul.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Cholesterol oxidation products frequently have a high biological activity. In the present study, we have used microelectrode recording of end plate currents and FM-based optical detection of synaptic vesicle exo-endocytosis to investigate the effects of two structurally similar oxysterols, olesoxime (cholest-4-en-3-one, oxime) and 5É‘-cholestan-3-one (5É‘Ch3), on neurotransmission at the frog neuromuscular junction. Olesoxime is an exogenous, potentially neuroprotective, substance and 5É‘Ch3 is an intermediate product in cholesterol metabolism, which is elevated in the case of cerebrotendinous xanthomatosis. We found that olesoxime slightly increased evoked neurotransmitter release in response to a single stimulus and significantly reduced synaptic depression during high frequency activity. The last effect was due to an increase in both the number of synaptic vesicles involved in exo-endocytosis and the rate of synaptic vesicle recycling. In contrast, 5É‘Ch3 reduced evoked neurotransmitter release during the low- and high frequency synaptic activities. The depressant action of 5É‘Ch3 was associated with a reduction in the number of synaptic vesicles participating in exo- and endocytosis during high frequency stimulation, without a change in rate of the synaptic vesicle recycling. Of note, olesoxime increased the staining of synaptic membranes with the B-subunit of cholera toxin and the formation of fluorescent ganglioside GM1 clusters, and decreased the fluorescence of 22-NBD-cholesterol, while 5É‘Ch3 had the opposite effects, suggesting that the two oxysterols have different effects on lipid raft stability. Taken together, these data show that these two structurally similar oxysterols induce marked different changes in neuromuscular transmission which are related with the alteration in synaptic vesicle cycle.
[Mh] Termos MeSH primário: Colestanos/farmacologia
Colestenonas/farmacologia
Potenciais Pós-Sinápticos Excitadores/efeitos dos fármacos
Músculo Esquelético/efeitos dos fármacos
Junção Neuromuscular/efeitos dos fármacos
Transmissão Sináptica/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Toxina da Cólera/química
Endocitose/efeitos dos fármacos
Potenciais Pós-Sinápticos Excitadores/fisiologia
Exocitose/efeitos dos fármacos
Gangliosídeo G(M1)/análogos & derivados
Gangliosídeo G(M1)/química
Microdomínios da Membrana/efeitos dos fármacos
Microeletrodos
Músculo Esquelético/inervação
Músculo Esquelético/fisiologia
Junção Neuromuscular/fisiologia
Rana ridibunda
Coloração e Rotulagem
Vesículas Sinápticas/efeitos dos fármacos
Técnicas de Cultura de Tecidos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cholestanes); 0 (Cholestenones); 0 (ganglioside GM1alpha); 37758-47-7 (G(M1) Ganglioside); 9012-63-9 (Cholera Toxin); A6778U5IFY (olesoxime); H6B6EPA52O (coprostanone)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160423
[St] Status:MEDLINE


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[PMID]:27101075
[Au] Autor:Pujol CA; Sepúlveda CS; Richmond V; Maier MS; Damonte EB
[Ad] Endereço:Laboratorio de Virología, Departamento de Química Biológica- IQUIBICEN (CONICET-UBA), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, Piso 4, 1428, Buenos Aires, Argentina.
[Ti] Título:Polyhydroxylated sulfated steroids derived from 5α-cholestanes as antiviral agents against herpes simplex virus.
[So] Source:Arch Virol;161(7):1993-9, 2016 Jul.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Twelve polyhydroxylated sulfated steroids synthesized from a 5α-cholestane skeleton with different substitutions in C-2, C-3 and C-6 were evaluated for cytotoxicity and antiviral activity against herpes simplex virus (HSV) by a virus plaque reduction assay. Four compounds elicited a selective inhibitory effect against HSV. The disodium salt of 2ß,3α-dihydroxy-6E-hydroximine-5α-cholestane-2,3-disulfate, named compound 7, was the most effective inhibitor of HSV-1, HSV-2 and pseudorabies virus (PrV) strains, including acyclovir-resistant variants, in human and monkey cell lines. Preliminary mechanistic studies demonstrated that compound 7 did not affect the initial steps of virus entry but inhibited a subsequent event in the infection process of HSV.
[Mh] Termos MeSH primário: Antivirais/farmacologia
Colestanos/farmacologia
Herpesvirus Humano 1/efeitos dos fármacos
Herpesvirus Humano 2/efeitos dos fármacos
Esteroides/farmacologia
[Mh] Termos MeSH secundário: Animais
Antivirais/química
Linhagem Celular
Colestanos/química
Herpes Genital/virologia
Herpes Simples/virologia
Herpesvirus Humano 1/fisiologia
Herpesvirus Humano 2/fisiologia
Seres Humanos
Estrutura Molecular
Esteroides/química
Relação Estrutura-Atividade
Internalização do Vírus/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Cholestanes); 0 (Steroids)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170203
[Lr] Data última revisão:
170203
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160422
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-016-2867-y


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[PMID]:27056839
[Au] Autor:Lagari VS; Levis S
[Ad] Endereço:University of Miami School of Medicine, Miami, Florida, USA; Miami Veterans Affairs Healthcare System, Miami, Florida, USA.
[Ti] Título:Implementation of a feasible monthly vitamin D intervention in homebound older adults using a Meals-on-Wheels programme.
[So] Source:Evid Based Nurs;19(3):96, 2016 07.
[Is] ISSN:1468-9618
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Pacientes Domiciliares
Vitamina D
[Mh] Termos MeSH secundário: Colestanos
Serviços de Alimentação
Seres Humanos
Vitaminas
[Pt] Tipo de publicação:JOURNAL ARTICLE; COMMENT
[Nm] Nome de substância:
0 (Cholestanes); 0 (Vitamins); 1406-16-2 (Vitamin D)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170503
[Lr] Data última revisão:
170503
[Sb] Subgrupo de revista:IM; N
[Da] Data de entrada para processamento:160409
[St] Status:MEDLINE
[do] DOI:10.1136/eb-2016-102331


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[PMID]:26976651
[Au] Autor:Rárová L; Steigerová J; Kvasnica M; Bartunek P; Krízová K; Chodounská H; Kolár Z; Sedlák D; Oklestkova J; Strnad M
[Ad] Endereço:Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University, Slechtitelu 27, 78371 Olomouc, Czech Republic. Electronic address: lucie.rarova@upol.cz.
[Ti] Título:Structure activity relationship studies on cytotoxicity and the effects on steroid receptors of AB-functionalized cholestanes.
[So] Source:J Steroid Biochem Mol Biol;159:154-69, 2016 May.
[Is] ISSN:1879-1220
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Structure-activity relationship analysis and profiling of a library of AB-functionalized cholestane derivatives closely related to brassinosteroids (BRs) were performed to examine their antiproliferative activities and activities on steroid hormone receptors. Some of the compounds were found to have strong cytotoxic activity in several human normal and cancer cell lines. The presence of a 3-hydroxy or 3-oxo group and 2,3-vicinal diol or 3,4-vicinal diol moiety were found to be necessary for optimum biological activity, as well as a six-membered B ring. According to the profiling of all steroid receptors in both agonist and antagonist mode, the majority of the cholestanes were weakly active or inactive compared to the natural ligands. Estrogenic activity was detected for two compounds, two compounds possessed antagonistic properties on estrogen receptors and seven compounds showed agonistic activity. Two active cholestane derivatives were shown to strongly influence cell viability, proliferation, cell cycle distribution, apoptosis and molecular pathways responsible for these processes in hormone-sensitive/insensitive (MCF7/MDA-MB-468) breast cancer cell lines.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Colestanos/farmacologia
Receptores de Esteroides/metabolismo
[Mh] Termos MeSH secundário: Antineoplásicos/química
Apoptose
Ciclo Celular/efeitos dos fármacos
Linhagem Celular Tumoral
Sobrevivência Celular
Colestanos/química
Ensaios de Seleção de Medicamentos Antitumorais
Seres Humanos
Concentração Inibidora 50
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Cholestanes); 0 (Receptors, Steroid)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:160404
[Lr] Data última revisão:
160404
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160316
[St] Status:MEDLINE


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[PMID]:26827630
[Au] Autor:D'yakonov VA; Tuktarova RA; Islamov II; Khalilov LM; Dzhemilev UM
[Ad] Endereço:Institute of Petrochemistry and Catalysis, Russian Academy of Sciences, pr. Oktyabrya 141, 450075 Ufa, Russian Federation. Electronic address: DyakonovVA@rambler.ru.
[Ti] Título:Catalytic cyclometallation in steroid chemistry IV: Efficient method for the synthesis of tetrahydrothiophene, tetrahydroselenophen and cyclopentanone derivatives of (5α)-cholestane.
[So] Source:Steroids;108:77-84, 2016 Apr.
[Is] ISSN:1878-5867
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Catalytic cycloalumination of (3ß,5α)-3-vinylcholestane and (3α,5α)-3-allylcholestane with Et3Al catalyzed by Cp2ZrCl2 was performed for the first time to give previously unknown aluminacyclopentanes in ∼90% yield; these products were converted in situ to carbo- and heterocyclic (5α)-cholestane derivatives.
[Mh] Termos MeSH primário: Colestanos/química
Colestanos/síntese química
Ciclopentanos/química
Compostos Organosselênicos/química
Tiofenos/química
[Mh] Termos MeSH secundário: Catálise
Técnicas de Química Sintética
Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cholestanes); 0 (Cyclopentanes); 0 (Organoselenium Compounds); 0 (Thiophenes); 0 (tetrahydroselenophen); 220W81TN3S (cyclopentanone); 744EHT13FM (tetrahydrothiophene)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160202
[St] Status:MEDLINE


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[PMID]:26790753
[Au] Autor:Romanello M; Zampieri S; Bortolotti N; Deroma L; Sechi A; Fiumara A; Parini R; Borroni B; Brancati F; Bruni A; Russo CV; Bordugo A; Bembi B; Dardis A
[Ad] Endereço:Regional Coordinator Centre for Rare Diseases, University Hospital Santa Maria della Misericordia, Udine, Italy. Electronic address: romanello.milena@aoud.sanita.fvg.it.
[Ti] Título:Comprehensive Evaluation of Plasma 7-Ketocholesterol and Cholestan-3ß,5α,6ß-Triol in an Italian Cohort of Patients Affected by Niemann-Pick Disease due to NPC1 and SMPD1 Mutations.
[So] Source:Clin Chim Acta;455:39-45, 2016 Apr 01.
[Is] ISSN:1873-3492
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Niemann-Pick C disease (NPCD) is a rare autosomal recessive neurovisceral disorder with a heterogeneous clinical presentation. Cholestan-3ß,5α,6ß-triol and 7-ketocholesterol have been proposed as biomarkers for the screening of NPCD. In this work, we assessed oxysterols levels in a cohort of Italian patients affected by NPCD and analyzed the obtained results in the context of the clinical, biochemical and molecular data. In addition, a group of patients affected by Niemann-Pick B disease (NPBD) were also analyzed. NPC patients presented levels of both oxysterols way above the cut off value, except for 5 siblings presenting the variant biochemical phenotype who displayed levels of 3ß,5α,6ß-triol below or just above the cut-off value; 2 of them presented also normal levels of 7-KC. Both oxysterols were extremely high in a patient presenting the neonatal systemic lethal phenotype. All NPB patients showed increased oxysterols levels. In conclusion, the reported LC-MS/MS assay provides a robust non-invasive screening tool for NPCD. However, false negative results can be obtained in patients expressing the variant biochemical phenotype. These data strengthen the concept that the results should always be interpreted in the context of the patients' clinical picture and filipin staining and/or genetic studies might still be undertaken in patients with normal levels of oxysterols if symptoms are highly suggestive of NPCD. Both oxysterols are significantly elevated in NPB patients; thus a differential diagnosis should always be performed in patients presenting isolated hepatosplenomegaly, a common clinical sign of both NPCD and NPBD.
[Mh] Termos MeSH primário: Proteínas de Transporte/genética
Colestanos/sangue
Cetocolesteróis/sangue
Glicoproteínas de Membrana/genética
Mutação
Doenças de Niemann-Pick/sangue
Esfingomielina Fosfodiesterase/genética
[Mh] Termos MeSH secundário: Calibragem
Estudos de Coortes
Seres Humanos
Itália
Doenças de Niemann-Pick/genética
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (Cholestanes); 0 (Ketocholesterols); 0 (Membrane Glycoproteins); 0 (NPC1 protein, human); EC 3.1.4.12 (Sphingomyelin Phosphodiesterase); EC 3.1.4.12 (sphingomyelin phosphodiesterase 1, human); O7676FE78M (7-ketocholesterol)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160122
[St] Status:MEDLINE



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