Base de dados : MEDLINE
Pesquisa : D04.210.500.247.222.222.347.833 [Categoria DeCS]
Referências encontradas : 649 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 65 ir para página                         

  1 / 649 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28480734
[Au] Autor:Kumar A; Chand G; Agnihotri VK
[Ad] Endereço:a Academy of Scientific and Innovative Research , CSIR-Institute of Himalayan Bioresource Technology , Palampur , India.
[Ti] Título:A new oxo-sterol derivative from the rhizomes of Costus speciosus.
[So] Source:Nat Prod Res;32(1):18-22, 2018 Jan.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Chemical investigation of the rhizomes of Costus speciosus led to the isolation of a new compound, 22-ketocholesteryl palmitate (1) along with four known compounds, 24-methylenecycloartanol (2), cycloartanol (3), stigmasterol (4) and linoleic acid (5). The structure of new compound was characterised by extensive 1D-, 2D-NMR and mass spectrometry (GC-MS and HR-ESI-MS) techniques.
[Mh] Termos MeSH primário: Costus/química
Cetosteroides/química
Rizoma/química
Esteróis/química
[Mh] Termos MeSH secundário: Cromatografia Gasosa-Espectrometria de Massas
Ácido Linoleico/química
Espectroscopia de Ressonância Magnética
Estrutura Molecular
Espectrometria de Massas por Ionização por Electrospray/métodos
Estigmasterol/química
Triterpenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (24-methylenecycloartenol); 0 (Ketosteroids); 0 (Sterols); 0 (Triterpenes); 99WUK5D0Y8 (Stigmasterol); 9KJL21T0QJ (Linoleic Acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170509
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2017.1324962


  2 / 649 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29345907
[Au] Autor:Alvarez-Sala A; López-García G; Attanzio A; Tesoriere L; Cilla A; Barberá R; Alegría A
[Ad] Endereço:Nutrition and Food Science Area, Faculty of Pharmacy, University of Valencia , Avda. Vicente Andrés Estellés s/n, Burjassot, Valencia 46100, Spain.
[Ti] Título:Effects of Plant Sterols or ß-Cryptoxanthin at Physiological Serum Concentrations on Suicidal Erythrocyte Death.
[So] Source:J Agric Food Chem;66(5):1157-1166, 2018 Feb 07.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The eryptotic and hemolytic effects of a phytosterol (PS) mixture (ß-sitosterol, campesterol, stigmasterol) or ß-cryptoxanthin (ß-Cx) at physiological serum concentration and their effect against oxidative stress induced by tert-butylhydroperoxide (tBOOH) (75 and 300 µM) were evaluated. ß-Cryptoxanthin produced an increase in eryptotic cells, cell volume, hemolysis, and glutathione depletion (GSH) without ROS overproduction and intracellular Ca influx. Co-incubation of both bioactive compounds protected against ß-Cx-induced eryptosis. Under tBOOH stress, PS prevented eryptosis, reducing Ca influx, ROS overproduction and GSH depletion at 75 µM, and hemolysis at both tBOOH concentrations. ß-Cryptoxanthin showed no cytoprotective effect. Co-incubation with both bioactive compounds completely prevented hemolysis and partially prevented eryptosis as well as GSH depletion induced by ß-Cx plus tBOOH. Phytosterols at physiological serum concentrations help to prevent pro-eryptotic and hemolytic effects and are promising candidate compounds for ameliorating eryptosis-associated diseases.
[Mh] Termos MeSH primário: beta-Criptoxantina/farmacologia
Eriptose/efeitos dos fármacos
Fitosteróis/farmacologia
[Mh] Termos MeSH secundário: beta-Criptoxantina/sangue
Células Cultivadas
Colesterol/análogos & derivados
Colesterol/farmacologia
Eritrócitos/química
Eritrócitos/efeitos dos fármacos
Glutationa/sangue
Hemólise/efeitos dos fármacos
Seres Humanos
Estresse Oxidativo/efeitos dos fármacos
Sitosteroides/farmacologia
Estigmasterol/farmacologia
terc-Butil Hidroperóxido/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Beta-Cryptoxanthin); 0 (Phytosterols); 0 (Sitosterols); 5L5O665639 (campesterol); 5LI01C78DD (gamma-sitosterol); 955VYL842B (tert-Butylhydroperoxide); 97C5T2UQ7J (Cholesterol); 99WUK5D0Y8 (Stigmasterol); GAN16C9B8O (Glutathione)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180119
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b05575


  3 / 649 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29232409
[Au] Autor:Kangsamaksin T; Chaithongyot S; Wootthichairangsan C; Hanchaina R; Tangshewinsirikul C; Svasti J
[Ad] Endereço:Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
[Ti] Título:Lupeol and stigmasterol suppress tumor angiogenesis and inhibit cholangiocarcinoma growth in mice via downregulation of tumor necrosis factor-α.
[So] Source:PLoS One;12(12):e0189628, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lupeol and stigmasterol, major phytosterols in various herbal plants, possess anti-inflammatory activities and have been proposed as candidates for anti-cancer agents, but their molecular mechanisms are still unclear. Here, we investigated the effects of lupeol and stigmasterol on tumor and endothelial cells in vitro and their anti-cancer activities in vivo. Our results demonstrated that lupeol and stigmasterol suppressed cell viability, migration, and morphogenesis of human umbilical vein endothelial cells (HUVECs) but not cholangiocarcinoma (CCA) cells. Expression analyses showed that the treatment of both compounds significantly reduced the transcript level of tumor necrosis factor-α (TNF-α), and Western blot analyses further revealed a decrease in downstream effector levels of VEGFR-2 signaling, including phosphorylated forms of Src, Akt, PCL, and FAK, which were rescued by TNF-α treatment. In vivo, lupeol and stigmasterol disrupted tumor angiogenesis and reduced the growth of CCA tumor xenografts. Immunohistochemical analyses confirmed a decrease in CD31-positive vessel content and macrophage recruitment upon treatment. These findings indicate that lupeol and stigmasterol effectively target tumor endothelial cells and suppress CCA tumor growth by their anti-inflammatory activities and are attractive candidates for anti-cancer treatment of CCA tumors.
[Mh] Termos MeSH primário: Proliferação Celular/efeitos dos fármacos
Colangiocarcinoma/patologia
Regulação para Baixo/efeitos dos fármacos
Neovascularização Patológica/prevenção & controle
Triterpenos Pentacíclicos/farmacologia
Estigmasterol/farmacologia
Fator de Necrose Tumoral alfa/metabolismo
[Mh] Termos MeSH secundário: Animais
Colangiocarcinoma/irrigação sanguínea
Células Endoteliais da Veia Umbilical Humana
Seres Humanos
Camundongos
Camundongos Endogâmicos C57BL
Transdução de Sinais
Fator A de Crescimento do Endotélio Vascular/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pentacyclic Triterpenes); 0 (Tumor Necrosis Factor-alpha); 0 (Vascular Endothelial Growth Factor A); 99WUK5D0Y8 (Stigmasterol); O268W13H3O (lupeol)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189628


  4 / 649 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28739601
[Au] Autor:Fialho MFP; Brusco I; da Silva Brum E; Piana M; Boligon AA; Trevisan G; Oliveira SM
[Ad] Endereço:Neurotoxicity and Psychopharmacology Laboratory, Center of Natural and Exact Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil.
[Ti] Título: Lam. crude extract presents antinociceptive effect on an arthritic pain model in mice.
[So] Source:Biochem J;474(17):2993-3010, 2017 Aug 17.
[Is] ISSN:1470-8728
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Arthritis is a chronic inflammatory disease which reduces the life quality of affected individuals. Therapeutic tools used for treating inflammatory pain are associated with several undesirable effects. Lam., known as 'Barbasco' or 'Cambara', is mostly used in several disorders and possesses antirheumatic, anti-inflammatory, and analgesic properties. Here, we investigated the antinociceptive and anti-inflammatory effects of the crude extract applied orally and topically in acute pain models and an arthritic pain model induced by complete Freund's adjuvant (CFA) paw injection in male mice (25-30 g). The high-performance liquid chromatography (HPLC) of the extract crude revealed the presence of the lupeol, stigmasterol, and ß-sitosterol. The stability study of the gel did not show relevant changes at low temperatures. The oral treatment with the extract prevented the capsaicin-induced spontaneous nociception and the acetic acid-induced abdominal writhing, but did not alter the thermal threshold in the tail immersion test. The antinociceptive effect was not reversed by naloxone in the capsaicin test. The oral or topical treatment reversed the CFA-induced mechanical allodynia and thermal hyperalgesia with maximum inhibition ( ) of 69 ± 6 and 68 ± 5% as well as 78 ± 15 and 87 ± 12%, respectively. Moreover, the topical but not oral treatment inhibited the CFA-induced cell infiltration, but did not reduce the paw edema significantly. The oral treatment with did not cause adverse effects. These findings suggest that the oral or topical treatment with presents antinociceptive actions in an arthritic pain model without causing adverse effects.
[Mh] Termos MeSH primário: Analgésicos não Entorpecentes/uso terapêutico
Anti-Inflamatórios não Esteroides/uso terapêutico
Artrite Experimental/tratamento farmacológico
Buddleja/química
Extratos Vegetais/uso terapêutico
Folhas de Planta/química
[Mh] Termos MeSH secundário: Dor Aguda/tratamento farmacológico
Administração Cutânea
Administração Oral
Analgésicos não Entorpecentes/administração & dosagem
Analgésicos não Entorpecentes/efeitos adversos
Analgésicos não Entorpecentes/química
Animais
Anti-Inflamatórios não Esteroides/administração & dosagem
Anti-Inflamatórios não Esteroides/efeitos adversos
Anti-Inflamatórios não Esteroides/química
Brasil
Buddleja/crescimento & desenvolvimento
Estabilidade de Medicamentos
Armazenamento de Medicamentos
Etnofarmacologia
Géis
Temperatura Alta/efeitos adversos
Masculino
Camundongos
Triterpenos Pentacíclicos/administração & dosagem
Triterpenos Pentacíclicos/efeitos adversos
Triterpenos Pentacíclicos/análise
Triterpenos Pentacíclicos/uso terapêutico
Extratos Vegetais/administração & dosagem
Extratos Vegetais/efeitos adversos
Extratos Vegetais/química
Folhas de Planta/crescimento & desenvolvimento
Sitosteroides/administração & dosagem
Sitosteroides/efeitos adversos
Sitosteroides/análise
Sitosteroides/uso terapêutico
Estigmasterol/administração & dosagem
Estigmasterol/efeitos adversos
Estigmasterol/análise
Estigmasterol/uso terapêutico
Viscosidade
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Analgesics, Non-Narcotic); 0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Gels); 0 (Pentacyclic Triterpenes); 0 (Plant Extracts); 0 (Sitosterols); 5LI01C78DD (gamma-sitosterol); 99WUK5D0Y8 (Stigmasterol); O268W13H3O (lupeol)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1042/BCJ20170008


  5 / 649 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28568378
[Au] Autor:Tahsin T; Wansi JD; Al-Groshi A; Evans A; Nahar L; Martin C; Sarker SD
[Ad] Endereço:Medicinal Chemistry and Natural Products Research Group, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, James Parsons Building, Byrom Street, Liverpool, L3 3AF, UK.
[Ti] Título:Cytotoxic Properties of the Stem Bark of Citrus reticulata Blanco (Rutaceae).
[So] Source:Phytother Res;31(8):1215-1219, 2017 Aug.
[Is] ISSN:1099-1573
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The bioassay-guided fractionation of the n-hexane extract of Citrus reticulata Blanco (Rutaceae) stem bark yielded scoparone (1), xanthyletin (2), lupeol (3), ß-amyrin (4), stigmasterol (5), ß-sitosterol (6) and palmitic acid. The structures of these compounds were determined by comprehensive spectroscopic analyses, i.e., 1D and 2D NMR and EI-MS, and by comparison with the reported data. Extracts, fractions and isolated compounds 1-6 were assessed for cytotoxicity by the 3-(4,5-dimethylthiazol-2-yl)-2,5-dphenyltetrazolium bromide (MTT) assay against three human cancer cell lines, i.e., human lung adenocarcinoma cell line A549, human breast adenocarcinoma cell line MCF7 and human Caucasian prostate adenocarcinoma cell line PC3. Significant activity of the n-hexane and the dichloromethane extracts was observed against the breast cancer cell line MCF7 with IC s of 45.6 and 54.7 µg/mL, respectively. Moreover, the 70% ethyl acetate in n-hexane chromatographic fraction showed significant activity displaying IC values of 53.0, 52.4 and 49.1 µg/mL against the cancer cell lines A549, MCF7 and PC3, respectively. Encouragingly, an IC of 510.0 µg/mL against the human normal prostate cell line PNT2 indicated very low toxicity and hence favourable selectivity indices for the 70% ethyl acetate in n-hexane fraction in the range of 9.6-10.4 towards cell lines A549, MCF7 and PC3. Because compounds isolated from the above fraction only delivered IC values in the range of 18.2-96.3, 9.2-34.1 and 7.5-97.2 µg/mL against A549, MCF7 and PC3 cell lines, respectively, synergistic action between compounds is suggested. Bioassay results valorize the anticancer effectivity of the stem bark of this plant in Cameroonian pharmacopoeia. Copyright © 2017 John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Citrus/química
Casca de Planta/química
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Linhagem Celular Tumoral
Fracionamento Químico
Cumarínicos
Seres Humanos
Ácido Oleanólico/análogos & derivados
Ácido Palmítico
Triterpenos Pentacíclicos
Extratos Vegetais/química
Sitosteroides
Estigmasterol
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Coumarins); 0 (Pentacyclic Triterpenes); 0 (Plant Extracts); 0 (Sitosterols); 2V16EO95H1 (Palmitic Acid); 3N789LD38N (xanthyletine); 5LI01C78DD (gamma-sitosterol); 6SMK8R7TGJ (Oleanolic Acid); 99WUK5D0Y8 (Stigmasterol); H5841PDT4Y (scoparone); KM8353IPSO (amyrin); O268W13H3O (lupeol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170602
[St] Status:MEDLINE
[do] DOI:10.1002/ptr.5842


  6 / 649 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28381771
[Au] Autor:Lodolini EM; Polverigiani S; Ali S; Mutawea M; Qutub M; Arabasi T; Pierini F; Abed M; Neri D
[Ad] Endereço:Dipartimento di Scienze Agrarie, Alimentari e Ambientali, Università Politecnica delle Marche.
[Ti] Título:Oil Characteristics of Four Palestinian Olive Varieties.
[So] Source:J Oleo Sci;66(5):435-441, 2017 May 01.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Olive oil represents an important source of income for Palestinian farmers in local, national and international markets. Sometimes, olive oil produced in local climatic conditions, does not achieve the International Olive Council (IOC) trade standards so that international markets are precluded. The oil chemical composition and sensory profile of four Palestinian olive varieties (Nabali Baladi, Nabali Mohassan, Souri and K18) were characterized in 2010 throughout an in situ evaluation. Most of the physicchemical characteristics and the fatty acid composition of the varieties met the International Olive Council trade standards (IOC-TS) for extra virgin olive oils. Values of K for Nabali Baladi and linolenic acid for Souri slightly exceeded the limit. Eicosanoic acid exceeded the IOC-TS limits in the oils of all considered varieties. Among the sterols, the Δ-7-stigmastenol resulted too high for Nabali Baladi and Souri. Sensory profile for the tested varieties showed a reminiscence of tomato or artichoke and light to medium bitter and pungent sensations. Results represent an important baseline reference for further studies about oil composition and quality of the main Palestinian olive germplasm and provide indication of potential critical points to be controlled in order to ensure the full achievement of IOC-TS and access international markets.
[Mh] Termos MeSH primário: Azeite de Oliva/química
Azeite de Oliva/normas
[Mh] Termos MeSH secundário: Árabes
Fenômenos Químicos
Ácidos Eicosanoicos/análise
Ácidos Graxos/análise
Qualidade dos Alimentos
Estigmasterol/análise
Paladar
Ácido alfa-Linolênico/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Eicosanoic Acids); 0 (Fatty Acids); 0 (Olive Oil); 0RBV727H71 (alpha-Linolenic Acid); 99WUK5D0Y8 (Stigmasterol)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess16184


  7 / 649 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28365392
[Au] Autor:Mouritsen OG; Bagatolli LA; Duelund L; Garvik O; Ipsen JH; Simonsen AC
[Ad] Endereço:MEMPHYS - Center for Biomembrane Physics, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark. Electronic address: ogm@memphys.sdu.dk.
[Ti] Título:Effects of seaweed sterols fucosterol and desmosterol on lipid membranes.
[So] Source:Chem Phys Lipids;205:1-10, 2017 Jun.
[Is] ISSN:1873-2941
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Higher sterols are universally present in large amounts (20-30%) in the plasma membranes of all eukaryotes whereas they are universally absent in prokaryotes. It is remarkable that each kingdom of the eukaryotes has chosen, during the course of evolution, its preferred sterol: cholesterol in animals, ergosterol in fungi and yeast, phytosterols in higher plants, and e.g., fucosterol and desmosterol in algae. The question arises as to which specific properties do sterols impart to membranes and to which extent do these properties differ among the different sterols. Using a range of biophysical techniques, including calorimetry, fluorescence microscopy, vesicle-fluctuation analysis, and atomic force microscopy, we have found that fucosterol and desmosterol, found in red and brown macroalgae (seaweeds), similar to cholesterol support liquid-ordered membrane phases and induce coexistence between liquid-ordered and liquid-disordered domains in lipid bilayers. Fucosterol and desmosterol induce acyl-chain order in liquid membranes, but less effectively than cholesterol and ergosterol in the order: cholesterol>ergosterol>desmosterol>fucosterol, possibly reflecting the different molecular structure of the sterols at the hydrocarbon tail.
[Mh] Termos MeSH primário: Desmosterol/química
Bicamadas Lipídicas/química
Alga Marinha/química
Estigmasterol/análogos & derivados
[Mh] Termos MeSH secundário: Varredura Diferencial de Calorimetria
Membrana Celular
Fenômenos Mecânicos
Microscopia de Força Atômica
Microscopia de Fluorescência
Estrutura Molecular
Estigmasterol/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipid Bilayers); 313-04-2 (Desmosterol); 504ZAM710C (fucosterol); 99WUK5D0Y8 (Stigmasterol)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170620
[Lr] Data última revisão:
170620
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170403
[St] Status:MEDLINE


  8 / 649 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28351722
[Au] Autor:Yang X; Zhou J; Wang T; Zhao L; Ye G; Shi F; Li Y; Tang H; Dong Q; Zhou X; Xu M; Rong Q; Chen H; Yang X; Cai Y
[Ad] Endereço:Department of Pharmacy, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
[Ti] Título:A novel method for synthesis of α-spinasterol and its antibacterial activities in combination with ceftiofur.
[So] Source:Fitoterapia;119:12-19, 2017 Jun.
[Is] ISSN:1873-6971
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this study, we designed a novel method of the synthesis of α-spinasterol from commercially available stigmasterol and explored the combinational effect of the α-spinasterol with ceftiofur in vitro against S. pullorum cvcc533, S. pneumoniae CAU0070, E. coli, and S. aureus. α-Spinasterol was obtained by a key reaction of Bamford-Stevens reaction with a desirable yield for five steps. The combination of α-spinasterol and ceftiofur showed stronger synergetic effect against the four pathogenic strains compared with that of stigmasterol and ceftiofur alone. In time-kill analyses, at concentrations above the MICs, ceftiofur in combination with α-spinasterol exhibited time-dependency and concentration-dependency comparing to time dependency with ceftiofur alone. We conclude that the combination usage of α-spinasterol and ceftiofur is an effective and promising strategy against the four pathogenic bacterial strains in vitro.
[Mh] Termos MeSH primário: Antibacterianos/síntese química
Antibacterianos/farmacologia
Cefalosporinas/farmacologia
Estigmasterol/análogos & derivados
[Mh] Termos MeSH secundário: Sinergismo Farmacológico
Escherichia coli/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Estrutura Molecular
Staphylococcus/efeitos dos fármacos
Estigmasterol/síntese química
Estigmasterol/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Cephalosporins); 0LG993QX1A (spinasterol); 83JL932I1C (ceftiofur); 99WUK5D0Y8 (Stigmasterol)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170615
[Lr] Data última revisão:
170615
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170330
[St] Status:MEDLINE


  9 / 649 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28317412
[Au] Autor:Biradi M; Hullatti K
[Ad] Endereço:a Department of Pharmaconosy , KLE University's College of Pharmacy , Belagavi , India.
[Ti] Título:Bioactivity guided isolation of cytotoxic terpenoids and steroids from Premna serratifolia.
[So] Source:Pharm Biol;55(1):1375-1379, 2017 Dec.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Despite several phytochemical studies of Premna serratifolia Linn. (Verbenaceae), the isolation of active constituents of this plant remains to be explored. OBJECTIVE: The study isolates cytotoxic terpenoids and steroids from the leaves of Premna serratifolia. MATERIALS AND METHODS: Unsaponifiable matter of hexane soluble fraction obtained from methanol extract was subjected to isolation by column chromatography and preparative TLC. Three compounds PS-01 A, PS-01B and PS-02 A were isolated. PS-01 A and PS-01B were identified by comparative TLC with authentic marker compounds followed by NMR analysis. Further PS-01B was analyzed by HR-GCMS. PS-02 A was subjected to HR-LCMS. All isolated compounds/fractions were evaluated for cytotoxic activity by BSL bioassay and using cell lines A549, HepG2 and L6. RESULTS: Three compounds were isolated from the leaf extract by bioactivity-guided fractionation. Two of which, namely, PS-01 A (oleanolic acid) and PS-02 A (unknown) were found to be terpenoids and PS-01B was identified as steroid (stigmasterol). PS-02 A compound is to be purified and characterized further. All three compounds PS-01 A, PS-01B, PS-02 A showed cytotoxicity by BSL bioassay (LC value of 54.49, 30.83, 16.32 ppm, respectively) and by cell line study where isolate PS-02 A has shown more cytotoxicity with LC values of 66.77 and 53.72 µg/mL with A549 and HepG2 cells, respectively, when compared with other isolates. CONCLUSION: Bioactivity guided fractionation of Premna serratifolia leaves succeeded into isolation of two terpenoids and one steroid compound with significant cytotoxic activity. Here we report the isolation of these cytotoxic terpenoids/steroids from this plant for the first time which could be developed as anticancer agents.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Neoplasias Hepáticas/tratamento farmacológico
Neoplasias Pulmonares/tratamento farmacológico
Ácido Oleanólico/farmacologia
Extratos Vegetais/farmacologia
Estigmasterol/farmacologia
Verbenaceae/química
[Mh] Termos MeSH secundário: Células A549
Animais
Antineoplásicos Fitogênicos/isolamento & purificação
Antineoplásicos Fitogênicos/toxicidade
Sobrevivência Celular/efeitos dos fármacos
Cromatografia em Camada Delgada
Relação Dose-Resposta a Droga
Cromatografia Gasosa-Espectrometria de Massas
Células Hep G2
Hexanos/química
Seres Humanos
Concentração Inibidora 50
Neoplasias Hepáticas/patologia
Neoplasias Pulmonares/patologia
Espectroscopia de Ressonância Magnética
Metanol/química
Fibras Musculares Esqueléticas/efeitos dos fármacos
Fibras Musculares Esqueléticas/patologia
Ácido Oleanólico/isolamento & purificação
Ácido Oleanólico/toxicidade
Fitoterapia
Extratos Vegetais/isolamento & purificação
Extratos Vegetais/toxicidade
Folhas de Planta/química
Plantas Medicinais
Ratos
Solventes/química
Estigmasterol/isolamento & purificação
Estigmasterol/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Hexanes); 0 (Plant Extracts); 0 (Solvents); 2DDG612ED8 (n-hexane); 6SMK8R7TGJ (Oleanolic Acid); 99WUK5D0Y8 (Stigmasterol); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170321
[St] Status:MEDLINE
[do] DOI:10.1080/13880209.2017.1301491


  10 / 649 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
[PMID]:28217808
[Au] Autor:Chen K; Wang CQ; Fan YQ; Han ZH; Wang Y; Gao L; Zeng HS
[Ad] Endereço:Department of Cardiology, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
[Ti] Título:[Lipid-lowering effect of seven traditional Chinese medicine monomers in zebrafish system].
[So] Source:Sheng Li Xue Bao;69(1):55-60, 2017 Feb 25.
[Is] ISSN:0371-0874
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:The present study aimed to study lipid-lowering effect of seven traditional Chinese medicine monomers in zebrafish system. Zebrafish were fed with high fat diet to establish a hyperlipemia model, then fasted and bathed with seven traditional Chinese medicine monomers stigmasterol, triacontanol, chrysophanol, vanillic acid, shikimic acid, polydatin and oleanolic acid respectively. The oil red O staining was used to detect the blood lipids of zebrafish. Serum total cholesterol and triglyceride levels were detected to validate the lipid-lowering effect. The result showed that a zebrafish model of hyperlipemia could be established by feeding larvae zebrafish with high fat diet. Among the seven traditional Chinese medicine monomers, chrysophanol had lipid-lowering effect. Chrysophanol significantly reduced serum total cholesterol and triglyceride levels in adult zebrafish fed with high fat diet. Chrysophanol accelerated peristalsis frequency of zebrafish intestine and the excretion of high fat food. It is concluded that chrysophanol has lipid- lowering effect in zebrafish, and the mechanism of the effect may be due to the roles of chrysophanol in reducing lipid absorption from gastrointestinal tract and accelerating the excretion of food.
[Mh] Termos MeSH primário: Hiperlipidemias/tratamento farmacológico
Hipolipemiantes/farmacologia
Medicina Tradicional Chinesa
[Mh] Termos MeSH secundário: Animais
Antraquinonas/farmacologia
Dieta Hiperlipídica
Álcoois Graxos/farmacologia
Glucosídeos/farmacologia
Larva
Lipídeos/sangue
Ácido Oleanólico/farmacologia
Ácido Chiquímico/farmacologia
Estigmasterol/farmacologia
Estilbenos/farmacologia
Ácido Vanílico/farmacologia
Peixe-Zebra
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthraquinones); 0 (Fatty Alcohols); 0 (Glucosides); 0 (Hypolipidemic Agents); 0 (Lipids); 0 (Stilbenes); 29MS2WI2NU (Shikimic Acid); 6SMK8R7TGJ (Oleanolic Acid); 767RD0E90B (1-triacontanol); 99WUK5D0Y8 (Stigmasterol); GM8Q3JM2Y8 (Vanillic Acid); N1ST8V8RR2 (chrysophanic acid); XM261C37CQ (polydatin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170221
[St] Status:MEDLINE



página 1 de 65 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde