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  1 / 1535 MEDLINE  
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[PMID]:27777042
[Au] Autor:Li YB; Li XR; Yang T; Wang JX; Zhao XF
[Ad] Endereço:Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong 250100, China.
[Ti] Título:The steroid hormone 20-hydroxyecdysone promotes switching from autophagy to apoptosis by increasing intracellular calcium levels.
[So] Source:Insect Biochem Mol Biol;79:73-86, 2016 12.
[Is] ISSN:1879-0240
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Autophagy regulates cell survival (or cell death in several cases), whereas apoptosis regulates cell death. However, the relationship between autophagy and apoptosis and the regulative mechanism is unclear. We report that steroid hormone 20-hydroxyecdysone (20E) promotes switching from autophagy to apoptosis by increasing intracellular calcium levels in the midgut of the lepidopteran insect Helicoverpa armigera. Autophagy and apoptosis sequentially occurred during midgut programmed cell death under 20E regulation, in which lower concentrations of 20E induced microtubule-associated protein 1 light chain 3-phosphatidylethanolamine (LC3-II, also known as autophagy-related gene 8, ATG8) expression and autophagy. High concentrations of 20E induced cleavage of ATG5 to NtATG5 and pro-caspase-3 to active caspase-3, which led to a switch from autophagy to apoptosis. Blocking autophagy by knockdown of ATG5, ATG7, or ATG12, or with the autophagy inhibitor 3-methyladenine, inhibited 20E-induced autophagy and apoptosis. Blocking apoptosis by using the apoptosis inhibitor Ac-DEVD-CHO did not prevent 20E-induced autophagy, suggesting that apoptosis relies on autophagy. ATG5 knockdown resulted in abnormal pupation and delayed pupation time. High concentrations of 20E induced high levels of intracellular Ca , NtATG5, and active caspase-3, which mediated the switch from autophagy to apoptosis. Blocking 20E-mediated increase of cellular Ca caused a decrease of NtATG5 and active caspase-3 and repressed the transformation from autophagy to apoptosis, thereby promoting cell survival. 20E induces an increase in the concentration of intracellular Ca , thereby switching autophagic cell survival to apoptotic cell death.
[Mh] Termos MeSH primário: Apoptose
Autofagia
Cálcio/metabolismo
Ecdisterona/metabolismo
Mariposas/fisiologia
[Mh] Termos MeSH secundário: Animais
Trato Gastrointestinal/fisiologia
Espaço Intracelular/metabolismo
Larva/crescimento & desenvolvimento
Larva/fisiologia
Mariposas/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
5289-74-7 (Ecdysterone); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161106
[St] Status:MEDLINE


  2 / 1535 MEDLINE  
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[PMID]:28854664
[Au] Autor:Su NY; Monteagudo EJ
[Ad] Endereço:Department of Entomology and Nematology, Ft. Lauderdale Research and Education Center, University of Florida, Institute of Food and Agricultural Sciences, Ft. Lauderdale, FL 33314.
[Ti] Título:Hyperecdysonism in the Formosan Subterranean Termite and Eastern Subterranean Termite (Isoptera: Rhinotermitidae).
[So] Source:J Econ Entomol;110(4):1736-1739, 2017 08 01.
[Is] ISSN:1938-291X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Effects of ecdysone, 20-hydroxyecdysone (20E), and an ecdysone agonist, halofenozide, were tested against the Formosan subterranean termite, Coptotermes formosanus Shiraki, and the eastern subterranean termite, Reticulitermes flavipes (Kollar), in a 12-d no choice assay. Approximately 22-26% of R. flavipes and C. formosanus exhibited symptoms of hyperecdysonism, that is, "jackknife" position, when exposed to ecdysone and 20E at 1,000 ppm, respectively. High mortalities were recorded for both termite species in ecdysone and 20E at 100 and 1,000 ppm, but only at 10,000 ppm for halofenizide. Termites are known to move back to the central nest before the onset of ecdysis, and those that ingested lethal doses of chitin synthesis inhibitors (CSIs) die near the royal pairs, which partially accounts for the success of CSI baits to eliminate subterranean termite colonies. Because ecdysteroids and their agonists induce molting in termites, incorporation of these compounds into baits could potentially achieve the same colony elimination. This study showed that lethal time (12 d) of ecdysteroids and ecdysone agonist is shorter than that of a CSI (45 d); hence, the baiting time should be reduced by more than a month when they are incorporated in termite baits.
[Mh] Termos MeSH primário: Benzoatos
Ecdisona
Ecdisterona
Hidrazinas
Controle de Insetos
Inseticidas
Isópteros
[Mh] Termos MeSH secundário: Animais
Relação Dose-Resposta a Droga
Muda/efeitos dos fármacos
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzoates); 0 (Hydrazines); 0 (Insecticides); 3604-87-3 (Ecdysone); 5289-74-7 (Ecdysterone); C81K20PELV (N-4-chlorobenzoyl-N'-benzoyl-N'-tert-butylhydrazine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.1093/jee/tox178


  3 / 1535 MEDLINE  
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[PMID]:28849998
[Au] Autor:Song Y; Evenseth LM; Iguchi T; Tollefsen KE
[Ad] Endereço:a Norwegian Institute for Water Research (NIVA) , Gaustadalléen , Oslo , Norway.
[Ti] Título:Release of chitobiase as an indicator of potential molting disruption in juvenile Daphnia magna exposed to the ecdysone receptor agonist 20-hydroxyecdysone.
[So] Source:J Toxicol Environ Health A;80(16-18):954-962, 2017.
[Is] ISSN:1528-7394
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:During arthropod molting, the old exoskeleton is degraded and recycled by the molting fluid. Chitobiase, a major chitinolytic enzyme in the molting fluid, has been widely used as a biomarker to indicate endocrine disruption of molting in arthropods under environmental stress. Although release of chitobiase was extensively studied in organisms exposed to molting-inhibiting chemicals, enzymic association with molting and response of the molting hormone receptor, ecdysone receptor (EcR), is not well understood. The present study was therefore conducted to identify potential linkages between release of chitobiase, molting frequency, and EcR activation in a freshwater crustacean Daphnia magna after short-term (96 hr) exposure to endogenous molting hormone 20-hydroxyecdysone (20E). A suite of bioassays was used for this purpose, including the chitobiase activity, molting frequency, viability, and in vitro EcR activation. Effect concentrations were compared between different assays analyzed. Results showed that exposure to 20E reduced chitobiase release and molting frequency in a concentration-dependent manner. Exposure to as low as 250 nM 20E significantly decreased release of chitobiase after 72 hr exposure, whereas adverse effects on molting frequency and incomplete molting-associated mortality required higher 20E exposure concentrations. The EcR reporter assay further demonstrated that as low as 100 nM 20E may activate EcR in vitro. Data suggest that release of chitobiase may be employed as a sensitive indicator of potential molting disruption in crustaceans after exposure to EcR agonists such as 20E.
[Mh] Termos MeSH primário: Acetilglucosaminidase/metabolismo
Daphnia/efeitos dos fármacos
Ecdisterona/toxicidade
Muda/efeitos dos fármacos
Receptores de Esteroides/metabolismo
[Mh] Termos MeSH secundário: Animais
Daphnia/crescimento & desenvolvimento
Disruptores Endócrinos/toxicidade
Determinação de Ponto Final
Feminino
Receptores de Esteroides/agonistas
Poluentes Químicos da Água/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Endocrine Disruptors); 0 (Receptors, Steroid); 0 (Water Pollutants, Chemical); 0 (ecdysone receptor); 5289-74-7 (Ecdysterone); EC 3.2.1.52 (Acetylglucosaminidase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE
[do] DOI:10.1080/15287394.2017.1352215


  4 / 1535 MEDLINE  
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[PMID]:28782527
[Au] Autor:Kaieda Y; Masuda R; Nishida R; Shimell M; O'Connor MB; Ono H
[Ad] Endereço:Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
[Ti] Título:Glue protein production can be triggered by steroid hormone signaling independent of the developmental program in Drosophila melanogaster.
[So] Source:Dev Biol;430(1):166-176, 2017 10 01.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Steroid hormones regulate life stage transitions, allowing animals to appropriately follow a developmental timeline. During insect development, the steroid hormone ecdysone is synthesized and released in a regulated manner by the prothoracic gland (PG) and then hydroxylated to the active molting hormone, 20-hydroxyecdysone (20E), in peripheral tissues. We manipulated ecdysteroid titers, through temporally controlled over-expression of the ecdysteroid-inactivating enzyme, CYP18A1, in the PG using the GeneSwitch-GAL4 system in the fruit fly Drosophila melanogaster. We monitored expression of a 20E-inducible glue protein gene, Salivary gland secretion 3 (Sgs3), using a Sgs3:GFP fusion transgene. In wild type larvae, Sgs3-GFP expression is activated at the midpoint of the third larval instar stage in response to the rising endogenous level of 20E. By first knocking down endogenous 20E levels during larval development and then feeding 20E to these larvae at various stages, we found that Sgs3-GFP expression could be triggered at an inappropriate developmental stage after a certain time lag. This stage-precocious activation of Sgs3 required expression of the Broad-complex, similar to normal Sgs3 developmental regulation, and a small level of nutritional input. We suggest that these studies provide evidence for a tissue-autonomic regulatory system for a metamorphic event independent from the primary 20E driven developmental progression.
[Mh] Termos MeSH primário: Drosophila melanogaster/crescimento & desenvolvimento
Drosophila melanogaster/metabolismo
Ecdisterona/metabolismo
Proteínas do Grude Salivar de Drosophila/metabolismo
[Mh] Termos MeSH secundário: Estruturas Animais/efeitos dos fármacos
Estruturas Animais/metabolismo
Animais
Proteínas de Fluorescência Verde/metabolismo
Larva/efeitos dos fármacos
Larva/crescimento & desenvolvimento
Mifepristona/farmacologia
Modelos Biológicos
Progesterona/análogos & derivados
Transdução de Sinais
Fatores de Tempo
Transgenes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Glue Proteins, Drosophila); 147336-22-9 (Green Fluorescent Proteins); 320T6RNW1F (Mifepristone); 4G7DS2Q64Y (Progesterone); 5289-74-7 (Ecdysterone)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170808
[St] Status:MEDLINE


  5 / 1535 MEDLINE  
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[PMID]:28621470
[Au] Autor:Lin Y; Liu H; Yang C; Gu J; Shen G; Zhang H; Chen E; Han C; Zhang Y; Xu Y; Wu J; Xia Q
[Ad] Endereço:State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
[Ti] Título:The POU homeodomain transcription factor POUM2 and broad complex isoform 2 transcription factor induced by 20-hydroxyecdysone collaboratively regulate vitellogenin gene expression and egg formation in the silkworm Bombyx mori.
[So] Source:Insect Mol Biol;26(5):496-506, 2017 Oct.
[Is] ISSN:1365-2583
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Vitellogenin (Vg) is a source of nutrition for embryo development. Our previous study showed that the silkworm (Bombyx mori) transcription factor broad complex isoform 2 (BmBrC-Z2) regulates gene expression of the Vg gene (BmVg) by induction with 20-hydroxyecdysone (20E). However, the mechanism by which 20E regulates BmVg expression was not clarified. In this study, cell transfection experiments showed that the BmVg promoter containing the POU homeodomain transcription factor POUM2 (POUM2) and BrC-Z2 cis-response elements (CREs) showed a more significant response to 20E than that harbouring only the BrC-Z2 or POUM2 CRE. An electrophoretic mobility shift assay and chromatin immunoprecipitation assay showed that BmPOUM2 could bind to the POUM2 CRE of the BmVg promoter. Over-expression of BmPOUM2 and BmBrC-Z2 in B. mori embryo-derived cell line (BmE) could enhance the activity of the BmVg promoter carrying both the POUM2 and BrC-Z2 CREs following 20E induction. Quantitative PCR and immunofluorescence histochemistry showed that the expression pattern and tissue localization of BmPOUM2 correspond to those of BmVg. Glutathione S-transferase pull-down and co-immunoprecipitation assays confirmed that BmPOUM2 interacts only with BmBrC-Z2 to regulate BmVg expression. Down-regulation of BmPOUM2 in female silkworm by RNA interference significantly reduced BmVg expression, leading to abnormal egg formation. In summary, these results indicate that BmPOUM2 binds only to BmBrC-Z2 to collaboratively regulate BmVg expression by 20E induction to control vitellogenesis and egg formation in the silkworm. Moreover, these findings suggest that homeodomain protein POUM2 plays a novel role in regulating insect vitellogenesis.
[Mh] Termos MeSH primário: Bombyx/metabolismo
Ecdisterona/metabolismo
Óvulo/crescimento & desenvolvimento
Fatores de Transcrição/metabolismo
Vitelogeninas/metabolismo
[Mh] Termos MeSH secundário: Animais
Bombyx/genética
Feminino
Regulação da Expressão Gênica
Proteínas de Insetos/genética
Proteínas de Insetos/metabolismo
Metamorfose Biológica
Regiões Promotoras Genéticas
Vitelogeninas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 0 (Transcription Factors); 0 (Vitellogenins); 5289-74-7 (Ecdysterone)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170617
[St] Status:MEDLINE
[do] DOI:10.1111/imb.12315


  6 / 1535 MEDLINE  
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[PMID]:28597953
[Au] Autor:Liu HW; Wang LL; Meng Z; Tang X; Li YS; Xia QY; Zhao P
[Ad] Endereço:State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
[Ti] Título:A clip domain serine protease involved in moulting in the silkworm, Bombyx mori: cloning, characterization, expression patterns and functional analysis.
[So] Source:Insect Mol Biol;26(5):507-521, 2017 Oct.
[Is] ISSN:1365-2583
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Clip domain serine proteases (CLIPs), characterized by one or more conserved clip domains, are essential components of extracellular signalling cascades in various biological processes, especially in innate immunity and the embryonic development of insects. Additionally, CLIPs may have additional non-immune functions in insect development. In the present study, the clip domain serine protease gene Bombyx mori serine protease 95 (BmSP95), which encodes a 527-residue protein, was cloned from the integument of B. mori. Bioinformatics analysis indicated that BmSP95 is a typical CLIP of the subfamily D and possesses a clip domain at the N terminus, a trypsin-like serine protease (tryp_spc) domain at the C terminus and a conserved proline-rich motif between these two domains. At the transcriptional level, BmSP95 is expressed in the integument during moulting and metamorphosis, and the expression pattern is consistent with the fluctuating 20-hydroxyecdysone (20E) titre in B. mori. At the translational level, BmSP95 protein is synthesized in the epidermal cells, secreted as a zymogen and activated in the moulting fluid. Immunofluorescence revealed that BmSP95 is distributed into the old endocuticle in the moulting stage. The expression of BmSP95 was upregulated by 20E. Moreover, expression of BmSP95 was downregulated by pathogen infection. RNA interference-mediated silencing of BmSP95 led to delayed moulting from pupa to moth. These results suggest that BmSP95 is involved in integument remodelling during moulting and metamorphosis.
[Mh] Termos MeSH primário: Bombyx/fisiologia
Proteínas de Insetos/metabolismo
Muda
Serina Proteases/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Bacillus
Beauveria
Ecdisterona/metabolismo
Proteínas de Insetos/genética
Proteínas de Insetos/isolamento & purificação
Larva/enzimologia
Dados de Sequência Molecular
Filogenia
Pupa/enzimologia
Interferência de RNA
Análise de Sequência de DNA
Serina Proteases/genética
Serina Proteases/isolamento & purificação
Serratia marcescens
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 5289-74-7 (Ecdysterone); EC 3.4.- (Serine Proteases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170610
[St] Status:MEDLINE
[do] DOI:10.1111/imb.12312


  7 / 1535 MEDLINE  
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[PMID]:28572000
[Au] Autor:Wang P; Zhuo XR; Tang L; Liu XS; Wang YF; Wang GX; Yu XQ; Wang JL
[Ad] Endereço:Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China.
[Ti] Título:C-type lectin interacting with ß-integrin enhances hemocytic encapsulation in the cotton bollworm, Helicoverpa armigera.
[So] Source:Insect Biochem Mol Biol;86:29-40, 2017 Jul.
[Is] ISSN:1879-0240
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The encapsulation reaction in invertebrates is analogous to granuloma formation in vertebrates, and this reaction is severely compromised when ecdysone signaling is blocked. However, the molecular mechanism underlying the encapsulation reaction and its regulation by ecdysone remains obscure. In our previous study, we found that the C-type lectin HaCTL3, from the cotton bollworm Helicoverpa armigera, is involved in anti-bacterial immune response, acting as a pattern recognition receptor (PRR). In the current study, we demonstrate that HaCTL3 is involved in defense against parasites and directly binds to the surface of nematodes. Our in vitro and in vivo studies indicate that HaCTL3 enhances hemocytic encapsulation and melanization, whereas H. armigera ß-integrin (Haß-integrin), located on the surface of hemocytes, participates in encapsulation. Additionally, co-immunoprecipitation experiments reveal HaCTL3 interacts with Haß-integrin, and knockdown of Haß-integrin leads to reduced encapsulation of HaCTL3-coated beads. These results indicate that Haß-integrin serves as a hemocytic receptor of HaCTL3 during the encapsulation reaction. Furthermore, we demonstrate that 20-hydroxyecdysone (20E) treatment dramatically induces the expression of HaCTL3, and knockdown of the 20E receptor (EcR)/ultraspiracle (USP), abrogates this response. Overall, this study provides the first evidence of the presence of a hemocytic receptor (Haß-integrin), that interacts with the PRR HaCTL3 to facilitate encapsulation reaction in insects and demonstrates the regulation of this process by the steroid hormone ecdysone.
[Mh] Termos MeSH primário: Interações Hospedeiro-Parasita/imunologia
Cadeias beta de Integrinas/metabolismo
Lectinas Tipo C/metabolismo
Mariposas/imunologia
Nematoides/imunologia
[Mh] Termos MeSH secundário: Animais
Ecdisterona
Hemócitos/metabolismo
Melaninas/metabolismo
Mariposas/metabolismo
Mariposas/parasitologia
Coelhos
Receptores de Esteroides/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Integrin beta Chains); 0 (Lectins, C-Type); 0 (Melanins); 0 (Receptors, Steroid); 0 (ecdysone receptor); 5289-74-7 (Ecdysterone)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170603
[St] Status:MEDLINE


  8 / 1535 MEDLINE  
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[PMID]:28490635
[Au] Autor:Zeng B; Huang Y; Xu J; Shiotsuki T; Bai H; Palli SR; Huang Y; Tan A
[Ad] Endereço:From the Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
[Ti] Título:The FOXO transcription factor controls insect growth and development by regulating juvenile hormone degradation in the silkworm, .
[So] Source:J Biol Chem;292(28):11659-11669, 2017 Jul 14.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Forkhead box O (FOXO) functions as the terminal transcription factor of the insulin signaling pathway and regulates multiple physiological processes in many organisms, including lifespan in insects. However, how FOXO interacts with hormone signaling to modulate insect growth and development is largely unknown. Here, using the transgene-based CRISPR/Cas9 system, we generated and characterized mutants of the silkworm FOXO ( ) to elucidate its physiological functions during development of this lepidopteran insect. The mutant (FOXO-M) exhibited growth delays from the first larval stage and showed precocious metamorphosis, pupating at the end of the fourth instar (trimolter) rather than at the end of the fifth instar as in the wild-type (WT) animals. However, different from previous reports on precocious metamorphosis caused by juvenile hormone (JH) deficiency in silkworm mutants, the total developmental time of the larval period in the FOXO-M was comparable with that of the WT. Exogenous application of 20-hydroxyecdysone (20E) or of the JH analog rescued the trimolter phenotype. RNA-seq and gene expression analyses indicated that genes involved in JH degradation but not in JH biosynthesis were up-regulated in the FOXO-M compared with the WT animals. Moreover, we identified several FOXO-binding sites in the promoter of genes coding for JH-degradation enzymes. These results suggest that FOXO regulates JH degradation rather than its biosynthesis, which further modulates hormone homeostasis to control growth and development in In conclusion, we have uncovered a pivotal role for FOXO in regulating JH signaling to control insect development.
[Mh] Termos MeSH primário: Bombyx/metabolismo
Hidrolases de Éster Carboxílico/metabolismo
Epóxido Hidrolases/metabolismo
Proteína Forkhead Box O1/metabolismo
Hormônios Juvenis/metabolismo
Metamorfose Biológica
Fosfotransferases (Aceptor do Grupo Álcool)/metabolismo
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Bombyx/efeitos dos fármacos
Bombyx/crescimento & desenvolvimento
Sistemas CRISPR-Cas
Hidrolases de Éster Carboxílico/genética
Ecdisterona/farmacologia
Indução Enzimática/efeitos dos fármacos
Epóxido Hidrolases/genética
Feminino
Proteína Forkhead Box O1/genética
Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos
Hidrólise/efeitos dos fármacos
Proteínas de Insetos/genética
Proteínas de Insetos/metabolismo
Hormônios Juvenis/química
Masculino
Metamorfose Biológica/efeitos dos fármacos
Metoprene/farmacologia
Muda/efeitos dos fármacos
Mutação
Fosfotransferases (Aceptor do Grupo Álcool)/genética
Regiões Promotoras Genéticas/efeitos dos fármacos
Elementos de Resposta/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Forkhead Box Protein O1); 0 (Insect Proteins); 0 (Juvenile Hormones); 5289-74-7 (Ecdysterone); 8B830OJ2UX (Methoprene); EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor)); EC 2.7.1.- (juvenile hormone diol kinase); EC 3.1.1.- (Carboxylic Ester Hydrolases); EC 3.1.1.- (juvenile hormone esterase); EC 3.3.2.- (Epoxide Hydrolases); EC 3.3.2.- (juvenile hormone epoxide hydrolase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170803
[Lr] Data última revisão:
170803
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170512
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.777797


  9 / 1535 MEDLINE  
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[PMID]:28479230
[Au] Autor:Thussagunpanit J; Jutamanee K; Homvisasevongsa S; Suksamrarn A; Yamagami A; Nakano T; Asami T
[Ad] Endereço:Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan; Department of Botany, Faculty of Science, 50 Kasetsart University, Ladyao, Chatuchak, Bangkok 10900, Thailand. Electronic address: ju
[Ti] Título:Characterization of synthetic ecdysteroid analogues as functional mimics of brassinosteroids in plant growth.
[So] Source:J Steroid Biochem Mol Biol;172:1-8, 2017 Sep.
[Is] ISSN:1879-1220
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Brassinosteroids (BRs) are plant steroidal hormones that play important roles in many stages of plant growth. Several plant species produce ecdysteroids, which are known as insect molting steroid hormones. In this study, we evaluated the biological activities of three hydroxysteroidal compounds, 20-hydroxyecdysone (ECD), 7,8-dihydro-8α-20-hydroxyecdysone (DHECD), and 7,8-dihydro-5α,8α-20-hydroxyecdysone (α-DHECD), and compared their activities with that of brassinolide (BL), the most potent BR. In rice, DHECD and α-DHECD enhanced the degree of lamina inclination, as do BRs. In Arabidopsis thaliana, DHECD and α-DHECD increased hypocotyl length in the wild-type, and also partially overcame the hypocotyl shortening in the wild-type caused by 0.3µM brassinazole, a specific BR biosynthesis inhibitor. DHECD and α-DHECD partially reduced dwarfism in the BR-biosynthesis-deficient mutant det2. Treatment with DHECD or α-DHECD downregulated the expression of the BR biosynthesis genes DWF4 and CPD, which are generally, suppressed by BR, and upregulated the expression of TCH4 and SAUR-AC1, which are generally promoted by BR. However, their regulated activities were less effective than BL. Moreover, the 10 M DHECD and α-DHECD induced the accumulation of dephosphorylated BIL1/BZR1 that enhanced BR signaling as a master transcription factor. In contrast, ECD did not affect rice lamina bending, Arabidopsis hypocotyl elongation, the expression levels of BR-related genes and BIL1/BZR1 phosphorylation status. Based on these results, we hypothesize that both DHECD and α-DHECD have functional activities similar to those of BR.
[Mh] Termos MeSH primário: Arabidopsis/efeitos dos fármacos
Materiais Biomiméticos/farmacologia
Brassinosteroides/farmacologia
Ecdisterona/farmacologia
Oryza/efeitos dos fármacos
Reguladores de Crescimento de Planta/farmacologia
Esteroides Heterocíclicos/farmacologia
[Mh] Termos MeSH secundário: Arabidopsis/genética
Arabidopsis/crescimento & desenvolvimento
Arabidopsis/metabolismo
Proteínas de Arabidopsis/genética
Proteínas de Arabidopsis/metabolismo
Materiais Biomiméticos/síntese química
Sistema Enzimático do Citocromo P-450/genética
Sistema Enzimático do Citocromo P-450/metabolismo
Ecdisterona/análogos & derivados
Ecdisterona/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Regulação da Expressão Gênica de Plantas
Glicosiltransferases/genética
Glicosiltransferases/metabolismo
Hipocótilo/efeitos dos fármacos
Hipocótilo/genética
Hipocótilo/crescimento & desenvolvimento
Hipocótilo/metabolismo
Proteínas Nucleares/genética
Proteínas Nucleares/metabolismo
Oryza/genética
Oryza/crescimento & desenvolvimento
Oryza/metabolismo
Fosforilação/efeitos dos fármacos
Reação em Cadeia da Polimerase em Tempo Real
Sementes/efeitos dos fármacos
Sementes/genética
Sementes/crescimento & desenvolvimento
Sementes/metabolismo
Transdução de Sinais
Esteroide Hidroxilases/genética
Esteroide Hidroxilases/metabolismo
Triazóis/antagonistas & inibidores
Triazóis/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (BZR1 protein, Arabidopsis); 0 (Brassinosteroids); 0 (DET2 protein, Arabidopsis); 0 (DWF4 protein, Arabidopsis); 0 (Nuclear Proteins); 0 (Plant Growth Regulators); 0 (SAUR-AC1 protein, Arabidopsis); 0 (Steroids, Heterocyclic); 0 (Triazoles); 5289-74-7 (Ecdysterone); 9035-51-2 (Cytochrome P-450 Enzyme System); EC 1.14.- (CPD protein, Arabidopsis); EC 1.14.- (Steroid Hydroxylases); EC 2.4.- (Glycosyltransferases); EC 2.4.1.- (TCH4 protein, Arabidopsis); N9XRW3TF90 (brassinazole); Y9IQ1L53OX (brassinolide)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170509
[St] Status:MEDLINE


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[PMID]:28428023
[Au] Autor:Ventura T; Bose U; Fitzgibbon QP; Smith GG; Shaw PN; Cummins SF; Elizur A
[Ad] Endereço:GeneCology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, 4 Locked Bag, Maroochydore, Queensland 4558, Australia. Electronic address: tventura@usc.edu.au.
[Ti] Título:CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development.
[So] Source:J Steroid Biochem Mol Biol;171:262-269, 2017 Jul.
[Is] ISSN:1879-1220
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cytochrome P450s (CYP450s) are a rapidly evolving family of enzymes, making it difficult to identify bona fide orthologs with notable lineage-specific exceptions. In ecdysozoans, a small number of the most conserved orthologs include enzymes which metabolize ecdysteroids. Ecdysone pathway components were recently shown in a decapod crustacean but with a notable absence of shade, which is important for converting ecdysone to its active form, 20-hydroxyecdysone (20HE), suggesting that another CYP450 performs a similar function in crustaceans. A CYPome temporal expression analysis throughout metamorphosis performed in this research highlights several un-annotated CYP450s displaying differential expression and provides information into expression patterns of annotated CYP450s. Using the expression patterns in the Eastern spiny lobster Sagmariasus verreauxi, followed by 3D modelling and finally activity assays in vitro, we were able to conclude that a group of CYP450s, conserved across decapod crustaceans, function as the insect shade. To emphasize the fact that these genes share the function with shade but are phylogenetically distinct, we name this enzyme system Shed.
[Mh] Termos MeSH primário: Proteínas de Artrópodes/metabolismo
Sistema Enzimático do Citocromo P-450/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Metamorfose Biológica
Modelos Moleculares
Palinuridae/enzimologia
[Mh] Termos MeSH secundário: Animais
Proteínas de Artrópodes/química
Proteínas de Artrópodes/genética
Células COS
Cercopithecus aethiops
Biologia Computacional
Sistema Enzimático do Citocromo P-450/química
Sistema Enzimático do Citocromo P-450/genética
Bases de Dados de Compostos Químicos
Bases de Dados Genéticas
Bases de Dados de Proteínas
Ecdisterona/química
Ecdisterona/metabolismo
Perfilação da Expressão Gênica
Hidroxilação
Anotação de Sequência Molecular
Estrutura Molecular
Peso Molecular
Palinuridae/crescimento & desenvolvimento
Filogenia
Conformação Proteica
Proteínas Recombinantes/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Proteins); 0 (Recombinant Proteins); 5289-74-7 (Ecdysterone); 9035-51-2 (Cytochrome P-450 Enzyme System)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170422
[St] Status:MEDLINE



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