Base de dados : MEDLINE
Pesquisa : D04.210.500.578.502 [Categoria DeCS]
Referências encontradas : 4786 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 479 ir para página                         

  1 / 4786 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28235766
[Au] Autor:Thomas JD; Longen CG; Oyer HM; Chen N; Maher CM; Salvino JM; Kania B; Anderson KN; Ostrander WF; Knudsen KE; Kim FJ
[Ad] Endereço:Department of Pharmacology & Physiology, Drexel University College of Medicine, Philadelphia, Pennsylvania.
[Ti] Título:Sigma1 Targeting to Suppress Aberrant Androgen Receptor Signaling in Prostate Cancer.
[So] Source:Cancer Res;77(9):2439-2452, 2017 May 01.
[Is] ISSN:1538-7445
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Suppression of androgen receptor (AR) activity in prostate cancer by androgen depletion or direct AR antagonist treatment, although initially effective, leads to incurable castration-resistant prostate cancer (CRPC) via compensatory mechanisms including resurgence of AR and AR splice variant (ARV) signaling. Emerging evidence suggests that Sigma1 (also known as sigma-1 receptor) is a unique chaperone or scaffolding protein that contributes to cellular protein homeostasis. We reported previously that some Sigma1-selective small molecules can be used to pharmacologically modulate protein homeostasis pathways. We hypothesized that these Sigma1-mediated responses could be exploited to suppress AR protein levels and activity. Here we demonstrate that treatment with a small-molecule Sigma1 inhibitor prevented 5α- dihydrotestosterone-mediated nuclear translocation of AR and induced proteasomal degradation of AR and ARV, suppressing the transcriptional activity and protein levels of both full-length and splice-variant AR. Consistent with these data, RNAi knockdown of Sigma1 resulted in decreased AR levels and transcriptional activity. Furthermore, Sigma1 physically associated with ARV7 and AR as well as full-length AR. Treatment of mice xenografted with ARV-driven CRPC tumors with a drug-like small-molecule Sigma1 inhibitor significantly inhibited tumor growth associated with elimination of AR and ARV7 in responsive tumors. Together, our data show that Sigma1 modulators can be used to suppress AR/ARV-driven prostate cancer cells via regulation of pharmacologically responsive Sigma1-AR/ARV interactions, both and .
[Mh] Termos MeSH primário: Neoplasias de Próstata Resistentes à Castração/tratamento farmacológico
Isoformas de Proteínas/genética
Receptores Androgênicos/genética
Receptores sigma/genética
[Mh] Termos MeSH secundário: 17-Cetosteroides/metabolismo
Antagonistas de Androgênios/administração & dosagem
Androgênios/metabolismo
Androstanóis/metabolismo
Animais
Linhagem Celular Tumoral
Seres Humanos
Masculino
Camundongos
Neoplasias de Próstata Resistentes à Castração/genética
Neoplasias de Próstata Resistentes à Castração/patologia
Receptores sigma/antagonistas & inibidores
Ensaios Antitumorais Modelo de Xenoenxerto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (17-Ketosteroids); 0 (5-dihydrorubrosterone); 0 (AR protein, human); 0 (Androgen Antagonists); 0 (Androgens); 0 (Androstanols); 0 (Protein Isoforms); 0 (Receptors, Androgen); 0 (Receptors, sigma); 0 (sigma-1 receptor)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171121
[Lr] Data última revisão:
171121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170226
[St] Status:MEDLINE
[do] DOI:10.1158/0008-5472.CAN-16-1055


  2 / 4786 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28234904
[Au] Autor:Siddiqui M; Ahmad MS; Wahab AT; Yousuf S; Fatima N; Naveed Shaikh N; Rahman AU; Choudhary MI
[Ad] Endereço:H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan.
[Ti] Título:Biotransformation of a potent anabolic steroid, mibolerone, with Cunninghamella blakesleeana, C. echinulata, and Macrophomina phaseolina, and biological activity evaluation of its metabolites.
[So] Source:PLoS One;12(2):e0171476, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Seven metabolites were obtained from the microbial transformation of anabolic-androgenic steroid mibolerone (1) with Cunninghamella blakesleeana, C. echinulata, and Macrophomina phaseolina. Their structures were determined as 10ß,17ß-dihydroxy-7α,17α-dimethylestr-4-en-3-one (2), 6ß,17ß-dihydroxy-7α,17α-dimethylestr-4-en-3-one (3), 6ß,10ß,17ß-trihydroxy-7α,17α-dimethylestr-4-en-3-one (4), 11ß,17ß-dihydroxy-(20-hydroxymethyl)-7α,17α-dimethylestr-4-en-3-one (5), 1α,17ß-dihydroxy-7α,17α-dimethylestr-4-en-3-one (6), 1α,11ß,17ß-trihydroxy-7α,17α-dimethylestr-4-en-3-one (7), and 11ß,17ß-dihydroxy-7α,17α-dimethylestr-4-en-3-one (8), on the basis of spectroscopic studies. All metabolites, except 8, were identified as new compounds. This study indicates that C. blakesleeana, and C. echinulata are able to catalyze hydroxylation at allylic positions, while M. phaseolina can catalyze hydroxylation of CH2 and CH3 groups of substrate 1. Mibolerone (1) was found to be a moderate inhibitor of ß-glucuronidase enzyme (IC50 = 42.98 ± 1.24 µM) during random biological screening, while its metabolites 2-4, and 8 were found to be inactive. Mibolerone (1) was also found to be significantly active against Leishmania major promastigotes (IC50 = 29.64 ± 0.88 µM). Its transformed products 3 (IC50 = 79.09 ± 0.06 µM), and 8 (IC50 = 70.09 ± 0.05 µM) showed a weak leishmanicidal activity, while 2 and 4 were found to be inactive. In addition, substrate 1 (IC50 = 35.7 ± 4.46 µM), and its metabolite 8 (IC50 = 34.16 ± 5.3 µM) exhibited potent cytotoxicity against HeLa cancer cell line (human cervical carcinoma). Metabolite 2 (IC50 = 46.5 ± 5.4 µM) also showed a significant cytotoxicity, while 3 (IC50 = 107.8 ± 4.0 µM) and 4 (IC50 = 152.5 ± 2.15 µM) showed weak cytotoxicity against HeLa cancer cell line. Compound 1 (IC50 = 46.3 ± 11.7 µM), and its transformed products 2 (IC50 = 43.3 ± 7.7 µM), 3 (IC50 = 65.6 ± 2.5 µM), and 4 (IC50 = 89.4 ± 2.7 µM) were also found to be moderately toxic to 3T3 cell line (mouse fibroblast). Interestingly, metabolite 8 showed no cytotoxicity against 3T3 cell line. Compounds 1-4, and 8 were also evaluated for inhibition of tyrosinase, carbonic anhydrase, and α-glucosidase enzymes, and all were found to be inactive.
[Mh] Termos MeSH primário: 17-Cetosteroides/metabolismo
Antineoplásicos/metabolismo
Antiprotozoários/metabolismo
Cunninghamella/metabolismo
Nandrolona/análogos & derivados
Saccharomycetales/metabolismo
Congêneres da Testosterona/metabolismo
[Mh] Termos MeSH secundário: 17-Cetosteroides/química
17-Cetosteroides/isolamento & purificação
17-Cetosteroides/farmacologia
Animais
Antineoplásicos/química
Antineoplásicos/isolamento & purificação
Antineoplásicos/farmacologia
Antiprotozoários/química
Antiprotozoários/isolamento & purificação
Antiprotozoários/farmacologia
Biotransformação
Anidrases Carbônicas/química
Sobrevivência Celular/efeitos dos fármacos
Cromatografia Líquida de Alta Pressão
Cunninghamella/química
Cunninghamella/efeitos dos fármacos
Glucuronidase/antagonistas & inibidores
Glucuronidase/química
Células HeLa
Seres Humanos
Hidroxilação
Leishmania major/efeitos dos fármacos
Leishmania major/crescimento & desenvolvimento
Camundongos
Estrutura Molecular
Monofenol Mono-Oxigenase/química
Células NIH 3T3
Nandrolona/química
Nandrolona/metabolismo
Nandrolona/farmacologia
Saccharomycetales/química
Saccharomycetales/efeitos dos fármacos
Congêneres da Testosterona/química
Congêneres da Testosterona/isolamento & purificação
Congêneres da Testosterona/farmacologia
alfa-Glucosidases/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (17-Ketosteroids); 0 (Antineoplastic Agents); 0 (Antiprotozoal Agents); 0 (Testosterone Congeners); 6PG9VR430D (Nandrolone); 9OGY4BOR8D (mibolerone); EC 1.14.18.1 (Monophenol Monooxygenase); EC 3.2.1.20 (alpha-Glucosidases); EC 3.2.1.31 (Glucuronidase); EC 4.2.1.1 (Carbonic Anhydrases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170225
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0171476


  3 / 4786 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27864020
[Au] Autor:Liu Y; Wang Y; Chen X; Wu Q; Wang M; Zhu D; Ma Y
[Ad] Endereço:Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China; National Engineering Laboratory for Industrial Enzymes and Tianjin Engineering Research Center of Biocatalytic Technology, T
[Ti] Título:Regio- and stereoselective reduction of 17-oxosteroids to 17ß-hydroxysteroids by a yeast strain Zygowilliopsis sp. WY7905.
[So] Source:Steroids;118:17-24, 2017 Feb.
[Is] ISSN:1878-5867
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The reduction of 17-oxosteroids to 17ß-hydroxysteroids is one of the important transformations for the preparation of many steroidal drugs and intermediates. The strain Zygowilliopsis sp. WY7905 was found to catalyze the reduction of C-17 carbonyl group of androst-4-ene-3,17-dione (AD) to give testosterone (TS) as the sole product by the constitutive 17ß-hydroxysteroid dehydrogenase (17ß-HSD). The optimal conditions for the reduction were pH 8.0 and 30°C with supplementing 10g/l glucose and 1% Tween 80 (w/v). Under the optimized transformation conditions, 0.75g/l AD was reduced to a single product TS with >90% yield and >99% diastereomeric excess (de) within 24h. This strain also reduced other 17-oxosteroids such as estrone, 3ß-hydroxyandrost-5-en-17-one and norandrostenedione, to give the corresponding 17ß-hydroxysteroids, while the C-3 and C-20 carbonyl groups were intact. The absence of by-products in this microbial 17ß-reduction would facilitate the product purification. As such, the strain might serve as a useful biocatalyst for this important transformation.
[Mh] Termos MeSH primário: 17-Cetosteroides/química
17-Cetosteroides/metabolismo
Hidroxiesteroides/química
Hidroxiesteroides/metabolismo
Saccharomycetales/metabolismo
[Mh] Termos MeSH secundário: Espectroscopia de Ressonância Magnética
Nandrolona/química
Nandrolona/metabolismo
Oxirredução
Testosterona/química
Testosterona/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (17-Ketosteroids); 0 (Hydroxysteroids); 3XMK78S47O (Testosterone); 6PG9VR430D (Nandrolone)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170830
[Lr] Data última revisão:
170830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161120
[St] Status:MEDLINE


  4 / 4786 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27714952
[Au] Autor:Ayotte C; Sylvestre A; Charlebois A; Poirier D
[Ad] Endereço:INRS-Institut Armand Frappier, Laboratoire de controle du dopage, Laval, Quebec, Canada.
[Ti] Título:Detection of 5α-androst-2-en-17-one and variants: Identification of main urinary metabolites in human urine samples by GC-MS and NMR.
[So] Source:Drug Test Anal;8(11-12):1174-1185, 2016 Nov.
[Is] ISSN:1942-7611
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two steroids were identified in a supplement named D-2 following the detection of unknown compounds during the routine testing of an athlete's sample. The main glucuroconjugated metabolites were isolated from this urine by high performance liquid chromatography (HPLC) following enzymatic hydrolysis and identified by gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) analyses as being 2α-hydroxy-5α-androst-3-en-17-one (M1) and 2ß,3α-dihydroxy-5α-androstan-17-one (M2). A third metabolite, 3α,4ß-dihydroxy-5α-androstan-17-one (M3) was also detected, however in lower amounts. The precursor steroids, 5α-androst-2-en-17-one (1) and 5α-androst-3-en-17-one (2) were present in the first D-2 products offered on the Internet. Later, the corresponding 17-hydroxyl compounds were offered as such or as esters (acetate, cypionate) in different relative ratios. Both M2 and M3 were synthesized from the trans-diaxial hydrolysis of the corresponding 2α,3α- and 3α,4α-epoxides (3). These were excreted in the hours following the controlled administration of the commercial product called D-2 R to a male volunteer and were also produced from the incubation of 1 and 2 with S9 liver fractions. Some preparations contain predominantly the alkene in C-2 and, therefore, an efficient detection method must include both primary metabolites M1 and M2. The latter was found equally in the fractions extracted following the enzymatic hydrolysis with ß-glucuronidase and the chemical solvolysis, which may ease its identification. Copyright © 2016 John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: 17-Cetosteroides/análise
17-Cetosteroides/urina
Androstenos/análise
Androstenos/urina
Cromatografia Líquida de Alta Pressão/métodos
Compostos de Epóxi/química
Esteroides/química
[Mh] Termos MeSH secundário: 17-Cetosteroides/química
17-Cetosteroides/metabolismo
Androstenos/química
Androstenos/metabolismo
Doping nos Esportes
Cromatografia Gasosa-Espectrometria de Massas/métodos
Seres Humanos
Espectroscopia de Ressonância Magnética
Esteroides/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (17-Ketosteroids); 0 (Androstenes); 0 (Epoxy Compounds); 0 (Steroids); 0 (androst-3-en-17-one); 963-75-7 (androst-2-en-17-one)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170404
[Lr] Data última revisão:
170404
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161008
[St] Status:MEDLINE
[do] DOI:10.1002/dta.2105


  5 / 4786 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27571131
[Au] Autor:Gonzalez Deniselle MC; Liere P; Pianos A; Meyer M; Aprahamian F; Cambourg A; Di Giorgio NP; Schumacher M; De Nicola AF; Guennoun R
[Ad] Endereço:Unité 1195 INSERM and University Paris-Sud and University Paris Saclay (P.L., A.P., F.A., A.C., M.S., R.G.), 94276 Kremlin-Bicêtre, France; Laboratory of Neuroendocrine Biochemistry (M.C.G.-D., M.M., A.F.D.N.) and Laboratory of Neuroendocrinology (N.P.D.G.), Instituto de Biologia y Medicina Experime
[Ti] Título:Steroid Profiling in Male Wobbler Mouse, a Model of Amyotrophic Lateral Sclerosis.
[So] Source:Endocrinology;157(11):4446-4460, 2016 Nov.
[Is] ISSN:1945-7170
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Wobbler mouse is an animal model for human motoneuron diseases, especially amyotrophic lateral sclerosis (ALS), used in the investigation of both pathology and therapeutic treatment. ALS is a fatal neurodegenerative disease, characterized by the selective and progressive death of motoneurons, leading to progressive paralysis. Previous limited studies have reported steroidal hormone dysregulation in Wobbler mouse and in ALS patients, suggesting endocrine dysfunctions which may be involved in the pathogenesis of the disease. In this study, we established a steroid profiling in brain, spinal cord, plasma, adrenal glands, and testes in 2-month-old male Wobbler mice and their littermates by gas chromatography coupled to mass spectrometry. Our results show in Wobbler mice the following: 1) a marked up-regulation of corticosterone levels in adrenal glands, plasma, spinal cord regions (cervical, thoracic, lumbar) and brain; 2) a strong decrease in T levels in the testis, plasma, spinal cord, and brain; and 3) increased levels of progesterone and especially of its reduced metabolites 5α-dihydroprogesterone, allopregnanolone, and 20α-dihydroprogesterone in the brain, spinal cord, and adrenal glands. Furthermore, Wobbler mice showed a hypothalamic-pituitary-gonadal hypoactivity. Interestingly, plasma concentrations of corticosterone and T correlate well with their respective levels in cervical spinal cord in both control and Wobbler mice. T down-regulation is probably the consequence of adrenal hyperactivity, and the up-regulation of progesterone and its reduced metabolites may correspond to an endogenous protective mechanism in response to motoneuron degeneration. Our findings suggest that increased levels of corticosterone and decreased levels of T in plasma could be a signature of motoneuron degeneration.
[Mh] Termos MeSH primário: Esclerose Amiotrófica Lateral/metabolismo
[Mh] Termos MeSH secundário: 17-Cetosteroides/sangue
17-Cetosteroides/metabolismo
Glândulas Suprarrenais/metabolismo
Esclerose Amiotrófica Lateral/sangue
Androstanóis/sangue
Androstanóis/metabolismo
Animais
Encéfalo/metabolismo
Corticosterona/sangue
Corticosterona/metabolismo
Modelos Animais de Doenças
Feminino
Cromatografia Gasosa-Espectrometria de Massas
Hormônio Liberador de Gonadotropina/metabolismo
Hormônio Luteinizante/sangue
Masculino
Camundongos
Neurônios Motores/metabolismo
Neurônios Motores/fisiologia
Pregnanolona/sangue
Pregnanolona/metabolismo
Progesterona/sangue
Progesterona/metabolismo
Medula Espinal/metabolismo
Testículo/metabolismo
Testosterona/sangue
Testosterona/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (17-Ketosteroids); 0 (5-dihydrorubrosterone); 0 (Androstanols); 33515-09-2 (Gonadotropin-Releasing Hormone); 3XMK78S47O (Testosterone); 4G7DS2Q64Y (Progesterone); 9002-67-9 (Luteinizing Hormone); BXO86P3XXW (Pregnanolone); W980KJ009P (Corticosterone)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170519
[Lr] Data última revisão:
170519
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:160830
[St] Status:MEDLINE


  6 / 4786 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27466384
[Au] Autor:Corbin CJ; Legacki EL; Ball BA; Scoggin KE; Stanley SD; Conley AJ
[Ad] Endereço:Department of Population Health and ReproductionSchool of Veterinary Medicine, University of California, Davis, California, USA.
[Ti] Título:Equine 5α-reductase activity and expression in epididymis.
[So] Source:J Endocrinol;231(1):23-33, 2016 Oct.
[Is] ISSN:1479-6805
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The 5α-reductase enzymes play an important role during male sexual differentiation, and in pregnant females, especially equine species where maintenance relies on 5α-reduced progesterone, 5α-dihydroprogesterone (DHP). Epididymis expresses 5α-reductases but was not studied elaborately in horses. Epididymis from younger and older postpubertal stallions was divided into caput, corpus and cauda and examined for 5α-reductase activity and expression of type 1 and 2 isoforms by quantitative real-time polymerase chain reaction (qPCR). Metabolism of progesterone and testosterone to DHP and dihydrotestosterone (DHT), respectively, by epididymal microsomal protein was examined by thin-layer chromatography and verified by liquid chromatography tandem mass spectrometry (LC-MS/MS). Relative inhibitory potencies of finasteride and dutasteride toward equine 5α-reductase activity were investigated. Pregnenolone was investigated as an additional potential substrate for 5α-reductase, suggested previously from in vivo studies in mares but never directly examined. No regional gradient of 5α-reductase expression was observed by either enzyme activity or transcript analysis. Results of PCR experiments suggested that type 1 isoform predominates in equine epididymis. Primers for the type 2 isoform were unable to amplify product from any samples examined. Progesterone and testosterone were readily reduced to DHP and DHT, and activity was effectively inhibited by both inhibitors. Using epididymis as an enzyme source, no experimental evidence was obtained supporting the notion that pregnenolone could be directly metabolized by equine 5α-reductases as has been suggested by previous investigators speculating on alternative metabolic pathways leading to DHP synthesis in placenta during equine pregnancies.
[Mh] Termos MeSH primário: 3-Oxo-5-alfa-Esteroide 4-Desidrogenase/fisiologia
Inibidores de 5-alfa Redutase/metabolismo
Epididimo/enzimologia
[Mh] Termos MeSH secundário: 17-Cetosteroides
Androstanóis
Animais
Di-Hidrotestosterona/metabolismo
Dutasterida/metabolismo
Feminino
Finasterida/metabolismo
Cavalos
Masculino
Gravidez
Pregnenolona/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (17-Ketosteroids); 0 (5-alpha Reductase Inhibitors); 0 (5-dihydrorubrosterone); 0 (Androstanols); 08J2K08A3Y (Dihydrotestosterone); 57GNO57U7G (Finasteride); 73R90F7MQ8 (Pregnenolone); EC 1.3.99.5 (3-Oxo-5-alpha-Steroid 4-Dehydrogenase); O0J6XJN02I (Dutasteride)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160729
[St] Status:MEDLINE
[do] DOI:10.1530/JOE-16-0175


  7 / 4786 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26145461
[Au] Autor:Li Y; Cai L; Dong JW; Xing Y; Duan WH; Zhou H; Ding ZT
[Ad] Endereço:Key Laboratory of Medicinal Chemistry for Nature Resource, Ministry of Education, School of Chemical Science and Technology, Yunnan University, Kunming, Yunnan 650091, People's Republic of China.
[Ti] Título:Innovative Approach to the Accumulation of Rubrosterone by Fermentation of Asparagus filicinus with Fusarium oxysporum.
[So] Source:J Agric Food Chem;63(29):6596-602, 2015 Jul 29.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Rubrosterone, possessing various remarkable bioactivities, is an insect-molting C19-steroid. However, only very small amounts are available for biological tests due to its limited content from plant sources. Fungi of genus Fusarium have been reported to have the ability to convert C27-steroids into C19-steroids. In this study, Asparagus filicinus, containing a high content of 20-hydroxyecdysone, was utilized to accumulate rubrosterone through solid fermentation by Fusarium oxysporum. The results showed that F. oxysporum had the ability to facilitate the complete biotransformation of 20-hydroxyecdysone to rubrosterone by solid-state fermentation. The present method could be an innovative and efficient approach to accumulate rubrosterone with an outstanding conversion ratio.
[Mh] Termos MeSH primário: 17-Cetosteroides/metabolismo
Androstanóis/metabolismo
Asparagus (Planta)/metabolismo
Ecdisterona/metabolismo
Fermentação
Fusarium/metabolismo
[Mh] Termos MeSH secundário: Asparagus (Planta)/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (17-Ketosteroids); 0 (Androstanols); 19466-41-2 (rubrosterone); 5289-74-7 (Ecdysterone)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150729
[Lr] Data última revisão:
150729
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150707
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.5b02570


  8 / 4786 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25759197
[Au] Autor:Mori E; Maehara K; Iwata H; Sakajo A; Tsuchiya M; Ozawa H; Morita A; Maekawa T; Saeki A
[Ad] Endereço:Graduate School of Nursing, Chiba University, Chiba, Japan.
[Ti] Título:Comparing older and younger Japanese primiparae: fatigue, depression and biomarkers of stress.
[So] Source:Int J Nurs Pract;21 Suppl 1:10-20, 2015 Mar.
[Is] ISSN:1440-172X
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:This cohort study of primiparae was conducted to answer the following questions: Do older (≧ 35 years) and younger (20-29 years) Japanese primiparous mothers differ when comparing biomarkers of stress and measures of fatigue and depression? Are there changes in fatigue, depression and stress biomarkers when comparing older and younger mothers during the postpartum period? The Postnatal Accumulated Fatigue Scale and the Edinburgh Postnatal Depression Scale were administered in a time-series method four times: shortly after birth and monthly afterwards. Assays to measure biomarkers of stress, urinary 17-ketosteroids, urinary 17-hydroxycorticosteroids and salivary chromogranin-A, were collected shortly after delivery and at 1 month postpartum in both groups and a third time in older mothers at the 4th month. Statistical testing showed very little difference in fatigue, depression or stress biomarkers between older and younger mothers shortly after birth or 1 month later. Accumulated fatigue and depression scores of older mothers were highest 1 month after delivery. Additional cohort studies are required to characterize physical/psychological well-being of older Japanese primiparae.
[Mh] Termos MeSH primário: Depressão Pós-Parto/etiologia
Fadiga/etiologia
Idade Materna
Período Pós-Parto
Estresse Psicológico/etiologia
[Mh] Termos MeSH secundário: 17-Hidroxicorticosteroides/urina
17-Cetosteroides/urina
Adulto
Biomarcadores/análise
Cromogranina A/metabolismo
Estudos de Coortes
Depressão Pós-Parto/diagnóstico
Feminino
Seres Humanos
Japão
Paridade
Saliva/metabolismo
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (17-Hydroxycorticosteroids); 0 (17-Ketosteroids); 0 (Biomarkers); 0 (Chromogranin A)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:N
[Da] Data de entrada para processamento:150312
[St] Status:MEDLINE
[do] DOI:10.1111/ijn.12392


  9 / 4786 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:24929815
[Au] Autor:Zhao ZB; Liu Y; Yao Y
[Ad] Endereço:State Key Laboratory of Urban Water Resource and Environment, Academy of Fundamental and Interdisciplinary Science, Harbin Institute of Technology, Harbin 150080, People's Republic of China.
[Ti] Título:Computational determination of binding structures and free energies of glucose 6-phosphate dehydrogenase with novel steroid inhibitors.
[So] Source:J Mol Graph Model;51:168-72, 2014 Jun.
[Is] ISSN:1873-4243
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Glucose 6-phosphate dehydrogenase (G6PD), the first and the rate-limiting enzyme in the pentose phosphate pathway (PPP), catalyzes the oxidation of G6P to 6-phosphogluconolactone and the reduction of NADP(+) to NADPH. Its key role in cancer promotes the development of a potent and selective inhibitor that might increase cancer cell death when combined with radiotherapy. In the present study, we investigated the detailed binding modes and binding free energies for G6PD interacting with a promising series of recently developed inhibitors, i.e., the steroid derivatives, by performing molecular docking, molecular dynamics (MD) simulations, and binding free energy calculations. The docking indicates that the inhibitors occupy the binding sites of both G6P and NADP(+). The calculated binding free energies on the basis of the MD-simulated enzyme-inhibitor complexes are in good agreement with the experimental activity data for all of the examined inhibitors. The valuable insights into the detailed enzyme-inhibitor binding including the important intermolecular interactions, e.g., the hydrogen bond interaction and the hydrophobic interaction, have been provided. The computational results provide new insights into future rational design of more potent inhibitors of G6PD as a treatment for cancer.
[Mh] Termos MeSH primário: Antineoplásicos/química
Inibidores Enzimáticos/química
Glucosefosfato Desidrogenase/antagonistas & inibidores
Simulação de Acoplamento Molecular
[Mh] Termos MeSH secundário: 17-Cetosteroides/química
Domínio Catalítico
Desenho de Drogas
Glucosefosfato Desidrogenase/química
Seres Humanos
Simulação de Dinâmica Molecular
Terapia de Alvo Molecular
Neoplasias/tratamento farmacológico
Ligação Proteica
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (17-Ketosteroids); 0 (Antineoplastic Agents); 0 (Enzyme Inhibitors); EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
[Em] Mês de entrada:1502
[Cu] Atualização por classe:140628
[Lr] Data última revisão:
140628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140616
[St] Status:MEDLINE


  10 / 4786 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:24842300
[Au] Autor:Bochem AE; Holleboom AG; Romijn JA; Hoekstra M; Dallinga GM; Motazacker MM; Hovingh GK; Kuivenhoven JA; Stroes ES
[Ad] Endereço:Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands.
[Ti] Título:Adrenal Function in females with low plasma HDL-C due to mutations in ABCA1 and LCAT.
[So] Source:PLoS One;9(5):e90967, 2014.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Adrenal steroidogenesis is essential for human survival and depends on the availability of the precursor cholesterol. Male subjects with low plasma levels of high density lipoprotein (HDL) cholesterol are characterized by decreased adrenal function. Whether this is also the case in female subjects with low plasma HDL-C levels is unresolved to date. FINDINGS: 15 female ATP binding cassette transporter AI (ABCAI) and 14 female lecithin-cholesterol acyltransferase (LCAT) were included in the study. HDL-C levels were 38% and 41% lower in ABCA1 and LCAT mutation carriers compared to controls, respectively. Urinary steroid excretion of 17-ketogenic steroids or 17-hydroxy corticosteroids did not differ between 15 female ABCA1 mutation carriers (p = 0.27 vs 0.30 respectively) and 30 matched normolipidemic controls or between 14 female LCAT mutation carriers and 28 matched normolipidemic controls (p = 0.10 and 0.14, respectively). Cosyntropin testing in an unselected subgroup of 8 ABCA1 mutation carriers and 3 LCAT mutation carriers did not reveal differences between carriers and controls. CONCLUSION: Adrenal function in females with molecularly defined low HDL-C levels is not different from controls. The discrepancy with the finding of impaired steroidogenesis in males with molecularly defined low HDL-C levels underscores the importance of gender specific analyses in cholesterol-related research.
[Mh] Termos MeSH primário: Transportador 1 de Cassete de Ligação de ATP/genética
Glândulas Suprarrenais/metabolismo
HDL-Colesterol/metabolismo
Fosfatidilcolina-Esterol O-Aciltransferase/metabolismo
[Mh] Termos MeSH secundário: 17-Cetosteroides/metabolismo
Adulto
Feminino
Seres Humanos
Meia-Idade
Mutação/genética
Fosfatidilcolina-Esterol O-Aciltransferase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (17-Ketosteroids); 0 (ABCA1 protein, human); 0 (ATP Binding Cassette Transporter 1); 0 (Cholesterol, HDL); EC 2.3.1.43 (Phosphatidylcholine-Sterol O-Acyltransferase)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140521
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0090967



página 1 de 479 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde