Base de dados : MEDLINE
Pesquisa : D04.210.500.745.745 [Categoria DeCS]
Referências encontradas : 330 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 33 ir para página                         

  1 / 330 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28073043
[Au] Autor:Yang Y; Hu X; Cheng L; Tang W; Zhao W; Yang Y; Zuo J
[Ad] Endereço:Laboratory of Immunology and Virology, Experiment Center For Science and Technology, Shanghai University of Traditional Chinese Medicine, China.
[Ti] Título:Periplocoside A ameliorated type II collagen-induced arthritis in mice via regulation of the balance of Th17/Treg cells.
[So] Source:Int Immunopharmacol;44:43-52, 2017 Mar.
[Is] ISSN:1878-1705
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Periplocoside A (PSA) has been extracted from the Chinese herbal medicine Periploca sepium Bge to treat rheumatoid arthritis (RA) via immune regulation. We previously found that PSA exhibits immunosuppressive activity both in vitro and in vivo. Balanced regulation of helper T 17 (Th17)/regulatory T (Treg) cells is the current therapeutic direction for the treatment of RA. The present study investigated the mechanism of PSA in treating collagen-induced arthritis (CIA). The therapeutic effects and potential pharmacological mechanisms of PSA were specifically clarified by examining its effects on CIA in DBA/1 mice. PSA administration significantly relieved the severity of the arthritis, and preventive administration of PSA reduced the incidence of arthritis in the mice with CIA and relieved joint damage in terms of morphology. PSA was also able to reduce the levels of anti-collagen II (CII) antibodies and pro-inflammatory cytokines in the serum. As a result, the proportion of Th17 cells decreased, and the proportion of Treg cells increased. A follow-up study of the ex vivo immunological reactions induced by a specific antigen found that PSA suppressed lymphocyte proliferation, inhibited the differentiation and reactivity of Th17 cells, and promoted the proportion of Treg cells among helper T cells. PSA also exhibited pharmacological effect in regulating the balance between Th17 and Treg cells in CIA through relevant signalling pathways. Thus, PSA played a specific role in CIA treatment. In particular, our results suggest that the therapeutic effects of PSA on RA are partially realized via the regulation of the balance of Th17/Treg cells.
[Mh] Termos MeSH primário: Artrite Experimental/tratamento farmacológico
Artrite Reumatoide/tratamento farmacológico
Glicosídeos/uso terapêutico
Imunossupressores/uso terapêutico
Articulações/efeitos dos fármacos
Pregnenos/uso terapêutico
Linfócitos T Reguladores/imunologia
Células Th17/imunologia
[Mh] Termos MeSH secundário: Animais
Formação de Anticorpos/efeitos dos fármacos
Diferenciação Celular/efeitos dos fármacos
Células Cultivadas
Colágeno Tipo II/imunologia
Seres Humanos
Articulações/imunologia
Masculino
Medicina Tradicional Chinesa
Camundongos
Camundongos Endogâmicos DBA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Collagen Type II); 0 (Glycosides); 0 (Immunosuppressive Agents); 0 (Pregnenes); 114828-46-5 (periplocoside A)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170512
[Lr] Data última revisão:
170512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170111
[St] Status:MEDLINE


  2 / 330 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27756147
[Au] Autor:Gupta MR; Kumar A; Khare NK
[Ad] Endereço:a Department of Chemistry , University of Lucknow , Lucknow , India.
[Ti] Título:Isolation and identification of two novel compounds from Marsdenia roylei and their quantum chemical calculations.
[So] Source:Nat Prod Res;31(7):749-757, 2017 Apr.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two new compounds 1 (Methyl 26-di-deoxy-6-methyl-ß-D-allohexopyranoside) and 3 (3, 11, 12, 20 tetra-O-acetyl-pregn-5-ene-14ß-ol) have been isolated from the chloroform-soluble extract of the whole plant of Marsdenia roylei (family: Asclepiadaceae) and their structures were determined by 1D, 2D NMR and ESI-MS as well as by chemical modification. We have calculated the molecular geometries, local reactivity descriptors by the density functional theory with B3LYP functional with basis set 6-311G+(d,2p) of 1, 3 and 4 (deacylated 3). The H and C NMR chemical shifts of 1, 3 and 4 were calculated using Gauge-Including Atomic Orbital approach and these values are correlated with the experimental observations.
[Mh] Termos MeSH primário: Marsdenia/química
[Mh] Termos MeSH secundário: Acilação
Clorofórmio/química
Glucosídeos
Espectroscopia de Ressonância Magnética
Modelos Moleculares
Estrutura Molecular
Extratos Vegetais/química
Pregnenos
Teoria Quântica
Espectrometria de Massas por Ionização por Electrospray
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3,11,12,20 tetra-O-acetyl-pregn-5-ene-14beta-ol); 0 (Glucosides); 0 (Plant Extracts); 0 (Pregnenes); 0 (methyl 26-di-deoxy-6-methyl-beta-D-allohexopyranoside); 7V31YC746X (Chloroform)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161021
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2016.1241999


  3 / 330 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:27153092
[Au] Autor:Feng M; He Z; Wang Y; Yan X; Zhang J; Hu Z; Wu W
[Ad] Endereço:Key Laboratory of Botanical Pesticide R & D in Shaanxi Province, Yangling 712100, Shaanxi, China. fengmx2010@126.com.
[Ti] Título:Isolation of the Binding Protein of Periplocoside E from BBMVs in Midgut of the Oriental Amyworm Mythimna separata Walker (Lepidoptera: Noctuidae) through Affinity Chromatography.
[So] Source:Toxins (Basel);8(5), 2016 05 04.
[Is] ISSN:2072-6651
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Periplocosides, which are insecticidal compounds isolated from the root bark of Periploca sepium Bunge, can affect the digestive system of insects. However, the mechanism though which periplocosides induces a series of symptoms remains unknown. In this study, affinity chromatography was conducted by coupling periplocoside E-semi-succinic acid ester with epoxy amino hexyl (EAH) sepharose 4B. Sodium dodecyl sulfonate-polyacrylamide gelelectrophoresis (SDS-PAGE) was performed to analyze the fraction eluted by periplocoside E. Eight binding proteins (luciferin 4-monooxygenase, aminopeptidase N, aminopeptidase N3, nicotinamide adenine dinucleotide health (NADH) dehydrogenase subunit 5, phosphatidylinositol 3-phosphate 3-phosphatase myotubularin, actin, uncharacterized family 31 glucosidase KIAA1161, and 2OG-Fe(2) oxygenase superfamily protein) were obtained and identified through liquid chromatography/quadrupole-time of flight-mass spectrometry (LC/Q-TOF-MS) analysis of the midgut epithelium cells of Mythimna separata larvae. Aminopeptidase N and N3 are potential putative targets of periplocosides. This study establishes the foundation for further research on the mechanism of action and target localization of periplocosides in agricultural pests.
[Mh] Termos MeSH primário: Proteínas de Transporte/isolamento & purificação
Proteínas de Insetos/isolamento & purificação
Lepidópteros
[Mh] Termos MeSH secundário: Animais
Cromatografia de Afinidade
Ésteres
Inseticidas
Intestinos/química
Larva
Microvilosidades
Oligossacarídeos
Pregnenos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (Esters); 0 (Insect Proteins); 0 (Insecticides); 0 (Oligosaccharides); 0 (Pregnenes); 0 (periplocoside E)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171122
[Lr] Data última revisão:
171122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160507
[St] Status:MEDLINE


  4 / 330 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27045628
[Au] Autor:Kasimanickam RK; Whittier WD; Hall JB; Kastelic JP
[Ad] Endereço:Department of Veterinary Clinical Sciences, Washington State University, Pullman, Washington, USA. Electronic address: ramkasi@vetmed.wsu.edu.
[Ti] Título:Estrous synchronization strategies to optimize beef heifer reproductive performance after reproductive tract scoring.
[So] Source:Theriogenology;86(3):831-8, 2016 Aug.
[Is] ISSN:1879-3231
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Three experiments comparing four estrous synchronization protocols were conducted to determine estrous expression rate and artificial insemination pregnancy rate (AI-PR) in heifers with a range (1-5) of reproductive tract scores (RTSs). At enrollment (Day 0), 1783 Angus cross beef heifers from six locations were given body condition score and RTS. The four protocols were: (1) HRTS-DPGF group-heifers with RTS 5 received prostaglandin F2α (PGF; Dinoprost 25 mg; im) on Days 0 and 14; (2) HRTS-CIDR-PGF group-heifers with RTS 5 received a CIDR (1.3-g progesterone) insert on Day 7, followed by CIDR removal and PGF on Day 14; (3) LRTS-CIDR-PGF group-heifers with RTS 4 or less received a CIDR insert on Day 7, followed by CIDR removal and PGF on Day 14; and (4) HRTS-Select-Synch group-heifers with RTS 5 received 100 µg of gonadorelin diacetate tetrahydrate (gonadotropin releasing homone; im) on Day 7 and PGF on Day 14. In all groups, heifers observed in estrus were artificially inseminated (within 120 hours after PGF) using the AM-PM rule. In Experiment 1, estrus expression rates were 82.2% (282/343) and 88.5% (184/208) for HRTS-DPGF and LRTS-CIDR-PGF, respectively (P < 0.05), whereas AI-PR were 51.3% (176/343) and 59.1% (123/208; P < 0.1). In Experiment 2, estrus expression rates were 79.6 (168/211), 86.9 (186/214) and 84.2% (176/209) for HRTS-DPGF, HRTS-CIDR-PGF, and LRTS-CIDR-PGF groups (P > 0.1) and AI-PR were 52.1 (110/211), 60.3 (129/214), and 58.4% (122/209; P > 0.05). In Experiment 3, estrus expression rates were 77.5 (131/169), 85.5 (142/166), and 83.3% (219/263) for HRTS-DPGF, HRTS-Select-Synch and LRTS-CIDR-PGF (P > 0.05) and AI-PR were 53.3 (90/169), 60.2 (100/166), and 58.6% (154/263; P > 0.1). Overall, estrus expression rates for HRTS-DPGF, HRTS-Select-Synch, LRTS-CIDR-PGF, and HRTS-CIDR-PGF groups were 80.4 (581/723), 85.5 (142/166), 85.1 (579/680), and 86.9% (186/214), respectively; higher for heifers in LRTS-CIDR-PGF and HRTS-CIDR-PGF groups compared to heifers in HRTS-DPGF group (P < 0.05). The AI-PR for heifers in HRTS-DPGF was lower (52.0 [376/723]) compared with HRTS-Select-Synch (60.2 [100/166]), LRTS-CIDR-PGF (58.7 [399/680]), and HRTS-CIDR-PGF (60.3 [129/214]); P < 0.05). In conclusion, heifers achieved greater AI-PR after CIDR-PGF or HRTS-Select-Synch estrous synchronization protocols. Even though acceptable AI-PRs achieved in heifers with RTS 5 that were subjected to a double PGF protocol, the reproductive performance was reduced compared with other protocols used in this study.
[Mh] Termos MeSH primário: Bovinos/fisiologia
Dinoprosta/farmacologia
Sincronização do Estro/métodos
Genitália Feminina/anatomia & histologia
Hormônio Liberador de Gonadotropina/farmacologia
Progesterona/farmacologia
[Mh] Termos MeSH secundário: Animais
Bovinos/anatomia & histologia
Dinoprosta/administração & dosagem
Esquema de Medicação
Quimioterapia Combinada
Feminino
Hormônio Liberador de Gonadotropina/administração & dosagem
Inseminação Artificial/veterinária
Gravidez
Pregnenos
Progesterona/administração & dosagem
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pregnenes); 2681-56-3 (20-hydroxy-3-oxo-4-pregnen-21-carboxylic acid); 33515-09-2 (Gonadotropin-Releasing Hormone); 4G7DS2Q64Y (Progesterone); B7IN85G1HY (Dinoprost)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170412
[Lr] Data última revisão:
170412
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160406
[St] Status:MEDLINE


  5 / 330 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26135914
[Au] Autor:Li JL; Zhou J; Chen ZH; Guo SY; Li CQ; Zhao WM
[Ad] Endereço:†Department of Natural Product Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
[Ti] Título:Bioactive C21 Steroidal Glycosides from the Roots of Cynanchum otophyllum That Suppress the Seizure-like Locomotor Activity of Zebrafish Caused by Pentylenetetrazole.
[So] Source:J Nat Prod;78(7):1548-55, 2015 Jul 24.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Six new C21 steroidal glycosides, cynotophyllosides A-F (1-6), together with 16 known compounds, were isolated from the roots of Cynanchum otophyllum. The structures of the new compounds were elucidated by spectroscopic analysis and chemical methods. The three major components, otophylloside F (15), otophylloside B (17), and rostratamine 3-O-ß-D-oleandropyranosyl-(1→4)-ß-D-cymaropyranosyl-(1→4)-ß-D-cymaropyranoside (18), suppressed the seizure-like locomotor activity caused by pentylenetetrazole in zebrafish. Preliminary structure-activity relation studies revealed that a pregnene skeleton with a C-12 ester group (ikemaoyl > cinnamoyl > hydroxy > p-hydroxybenzoyl) and a C-3 sugar chain consisting of three 2,6-dideoxysaccharide units is essential for this suppressive activity.
[Mh] Termos MeSH primário: Cynanchum/química
Medicamentos de Ervas Chinesas/isolamento & purificação
Medicamentos de Ervas Chinesas/farmacologia
Glicosídeos/química
Glicosídeos/isolamento & purificação
Glicosídeos/farmacologia
Atividade Motora/efeitos dos fármacos
Pentilenotetrazol/farmacologia
Pregnenos/isolamento & purificação
Pregnenos/farmacologia
Convulsões/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Modelos Animais de Doenças
Medicamentos de Ervas Chinesas/química
Estrutura Molecular
Ressonância Magnética Nuclear Biomolecular
Raízes de Plantas/química
Pregnenos/química
Relação Estrutura-Atividade
Peixe-Zebra
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 0 (Glycosides); 0 (Pregnenes); WM5Z385K7T (Pentylenetetrazole)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:150724
[Lr] Data última revisão:
150724
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150703
[St] Status:MEDLINE
[do] DOI:10.1021/np501058b


  6 / 330 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:26084169
[Au] Autor:Liu Y; Ouyang Y; Wang ZQ; Qiao L; Li S; Zhao SH; Liu MY
[Ti] Título:[A new C21 steroidal saponins from Periplocae Cortex].
[So] Source:Zhongguo Zhong Yao Za Zhi;40(3):455-7, 2015 Feb.
[Is] ISSN:1001-5302
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To study the chemical constituents of Periplocae Cortex, the separation and purification of 70% alcohol extract were carried out by column chromatographies on AB-8 macroporous resin, silica gel and preparative HPLC. The structure of the compounds were identified by NMR and TOF-MS. A new compound was isolated and identified as 21-O-methyl-Δ5-pregnene-3ß, 14ß, 17ß, 21-tetraol-20-one-3-O-ß-D-oleandropyranosyl(1-->4)-ß-D-cymaropyranosyl-(1-->4)-ß-D-cymaropyranosyl (1), named as periplocoside P.
[Mh] Termos MeSH primário: Glicosídeos/isolamento & purificação
Periploca/química
Pregnenos/isolamento & purificação
Saponinas/isolamento & purificação
[Mh] Termos MeSH secundário: Glicosídeos/química
Pregnenos/química
Saponinas/química
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Glycosides); 0 (Pregnenes); 0 (Saponins); 0 (periplocoside P)
[Em] Mês de entrada:1507
[Cu] Atualização por classe:150618
[Lr] Data última revisão:
150618
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150619
[St] Status:MEDLINE


  7 / 330 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:24838379
[Au] Autor:Hwang IH; Kulkarni R; Yang MH; Choo SJ; Zhou W; Lee SM; Jang TS; Jeong GS; Chang HW; Na M
[Ad] Endereço:Department of Chemistry, University of Iowa, Iowa City, IA, 52242, USA.
[Ti] Título:Complete NMR assignments of undegraded asterosaponins from Asterias amurensis.
[So] Source:Arch Pharm Res;37(10):1252-63, 2014 Oct.
[Is] ISSN:0253-6269
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Four asterosaponins, thornasteroside A (1), versicoside A (2), anasteroside B (3), and asteronylpentaglycoside sulfate (4), were isolated from the predatory starfish Asterias amurensis Lütken. Unlike previous studies focusing on structure elucidation by degradation of the complex saponin molecules, complete nuclear magnetic resonance (NMR) assignment for the intact molecules was accomplished using 600 MHz high magnetic field NMR. The complete set of NMR assignments can help in the structure elucidation of asterosaponins isolated in low yields without resorting to chemical degradation. Furthermore, this approach can be extended to other complex steroidal saponins, which may accelerate the discovery of bioactive secondary metabolites from this invasive starfish species.
[Mh] Termos MeSH primário: Colestenonas/química
Glicosídeos/química
Compostos Policíclicos/química
Pregnenos/química
Saponinas/química
[Mh] Termos MeSH secundário: Animais
Asterias
Colestenonas/isolamento & purificação
Colestenonas/farmacologia
Inibidores da Síntese de Ácidos Graxos/química
Inibidores da Síntese de Ácidos Graxos/isolamento & purificação
Inibidores da Síntese de Ácidos Graxos/farmacologia
Glicosídeos/isolamento & purificação
Glicosídeos/farmacologia
Espectroscopia de Ressonância Magnética
Estrutura Molecular
Compostos Policíclicos/isolamento & purificação
Compostos Policíclicos/farmacologia
Pregnenos/isolamento & purificação
Pregnenos/farmacologia
Saponinas/isolamento & purificação
Saponinas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cholestenones); 0 (Fatty Acid Synthesis Inhibitors); 0 (Glycosides); 0 (Polycyclic Compounds); 0 (Pregnenes); 0 (Saponins); 0 (anasteroside B); 0 (asterosaponin 1); 0 (thornasteroside A); 0 (versicoside A)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:140926
[Lr] Data última revisão:
140926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140520
[St] Status:MEDLINE
[do] DOI:10.1007/s12272-014-0374-9


  8 / 330 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
[PMID]:24658800
[Au] Autor:Yan Y; Rempel DL; Holy TE; Gross ML
[Ti] Título:Mass spectrometry combinations for structural characterization of sulfated-steroid metabolites.
[So] Source:J Am Soc Mass Spectrom;25(5):869-79, 2014 May.
[Is] ISSN:1879-1123
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Steroid conjugates, which often occur as metabolites, are challenging to characterize. One application is female-mouse urine, where steroid conjugates serve as important ligands for the pheromone-sensing neurons. Although the two with the highest abundance in mouse urine were previously characterized with mass spectrometry (MS) and NMR to be sulfated steroids, many more exist but remain structurally unresolved. Given that their physical and chemical properties are similar, they are likely to have a sulfated steroid ring structure. Because these compounds occur in trace amounts in mouse urine and elsewhere, their characterization by NMR will be difficult. Thus, MS methods become the primary approach for determining structure. Here, we show that a combination of MS tools is effective for determining the structures of sulfated steroids. Using 4-pregnene analogs, we explored high-resolving power MS (HR-MS) to determine chemical formulae; HD exchange MS (HDX-MS) to determine number of active, exchangeable hydrogens (e.g., OH groups); methoxyamine hydrochloride (MOX) derivatization MS, or reactive desorption electrospray ionization with hydroxylamine to determine the number of carbonyl groups; and tandem MS (MS(n)), high-resolution tandem MS (HRMS/MS), and GC-MS to obtain structural details of the steroid ring. From the fragmentation studies, we deduced three major fragmentation rules for this class of sulfated steroids. We also show that a combined MS approach is effective for determining structure of steroid metabolites, with important implications for targeted metabolomics in general and for the study of mouse social communication in particular.
[Mh] Termos MeSH primário: Hidrocortisona/análogos & derivados
Pregnenos/metabolismo
[Mh] Termos MeSH secundário: Métodos Analíticos de Preparação de Amostras
Animais
Feminino
Cromatografia Gasosa-Espectrometria de Massas
Hidrocortisona/química
Hidrocortisona/metabolismo
Hidroxilaminas/química
Indicadores e Reagentes/química
Camundongos
Estrutura Molecular
Peso Molecular
Pregnenos/química
Pregnenos/urina
Espectrometria de Massas por Ionização por Electrospray
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Hydroxylamines); 0 (Indicators and Reagents); 0 (Pregnenes); 1253-43-6 (cortisol 21-sulfate); 9TZH4WY30J (methoxyamine); WI4X0X7BPJ (Hydrocortisone)
[Em] Mês de entrada:1505
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140325
[St] Status:MEDLINE


  9 / 330 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:24648170
[Au] Autor:Wang HL; Qin N; Liu J; Jin MN; Zhang X; Jin MH; Kong D; Jiang SD; Duan HQ
[Ad] Endereço:School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, China.
[Ti] Título:Synthesis and antimetastatic effects of E-salignone amide derivatives.
[So] Source:Drug Dev Res;75(2):76-87, 2014 Mar.
[Is] ISSN:1098-2299
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The preparation of novel E-salignone derivatives and their biological evaluation as potential antimetastatic agents is described. The E-salignone amide derivatives were prepared from epiandrosterone and androsterone, and characterized by analytical (1) H NMR, (13) C NMR, and mass spectrometry. The derivatives were evaluated for antimetastatic activity in MDA-MB-231 cells by using a transwell assay. Comparing with the positive control, LY294002, compounds 19b, 19d, and 19e exhibited significant inhibitory effects on the EGF-induced invasion of MB-MDA-231 cells. Moreover, compound 19b also had antimigration effects in wound-healing assay. Compound 19b may represent a novel antimetastatic agent for treating breast cancer.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/síntese química
Antineoplásicos Fitogênicos/farmacologia
Neoplasias da Mama/prevenção & controle
Pregnenos/síntese química
Pregnenos/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/isolamento & purificação
Neoplasias da Mama/patologia
Movimento Celular/efeitos dos fármacos
Cromonas/farmacologia
Feminino
Seres Humanos
Estrutura Molecular
Morfolinas/farmacologia
Invasividade Neoplásica
Metástase Neoplásica
Pachysandra/química
Pregnenos/química
Pregnenos/isolamento & purificação
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Chromones); 0 (Morpholines); 0 (Pregnenes); 31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140321
[St] Status:MEDLINE
[do] DOI:10.1002/ddr.21157


  10 / 330 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:24258707
[Au] Autor:Misra A; Kushwaha HN; Gautam N; Singh B; Verma PC; Pratap R; Singh SK
[Ad] Endereço:Pharmacokinetics and Metabolism Division, CSIR-Central Drug Research Institute, Lucknow, India.
[Ti] Título:Bioanalytical LC-MS/MS method development and validation of novel antidiabetic candidate S007-1261 in rat plasma and its application to pharmacokinetic and oral bioavailability studies.
[So] Source:Drug Res (Stuttg);64(8):399-405, 2014 Aug.
[Is] ISSN:2194-9387
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A sensitive and selective LC-MS/MS method has been developed and validated for CDRI antidiabetic candidate S007-1261 in rat plasma using 16-dehydropregnenolone as an internal standard. The API 4000 triple quadrupole LC-MS/MS system was operated under multiple reaction monitoring mode using electrospray ionization technique in positive mode. The sample processing method involves 2-step liquid-liquid extraction using n-hexane as an extracting solvent. The analyte was chromatographed on RP 18, waters column (3.5 µm, 2.1 mm i.d. × 30 mm) with guard using acetonitrile and ammonium acetate buffer (pH 5.0, 10 mM) in 90:10 (v/v) composition at a flow rate of 0.40 mL min(-1). The chromatographic run time was 5.30 min. Calibration curve shows linearity over concentration range 1.56-200 ng mL(-1). The lower limit of detection was 0.39 ng mL(-1) and lower limit of quantitation was 1.56 ng mL(-1). The inter- and intra-day accuracy and precision were found to be within the assay variability limits as per US FDA guidelines. The absolute recovery of S007-1261 was found to be >90%. S007-1261 does not show any stability problems as it was stable at room temperature for 8 h. S007-1261 was also stable up to 3 freeze-thaw cycles and can be stored up to 30 days at -60 °C. The assay was successfully applied to both oral (40 mg kg(-1)) and intravenous (10 mg kg(-1)) pharmacokinetic studies in male Sprague-Dawley rats. The oral bioavailability of S007-1261 was found to be 33.61%.
[Mh] Termos MeSH primário: Hipoglicemiantes/sangue
Hipoglicemiantes/farmacocinética
Oximas/sangue
Oximas/farmacocinética
Pregnenos/sangue
Pregnenos/farmacocinética
[Mh] Termos MeSH secundário: Administração Oral
Animais
Disponibilidade Biológica
Calibragem
Meia-Vida
Hipoglicemiantes/administração & dosagem
Injeções Intravenosas
Masculino
Oximas/administração & dosagem
Pregnenos/administração & dosagem
Pregnenolona/análogos & derivados
Controle de Qualidade
Ratos
Ratos Sprague-Dawley
Padrões de Referência
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Hypoglycemic Agents); 0 (Oximes); 0 (Pregnenes); 0 (S007-1261); 7349506P5S (16-dehydropregnenolone); 73R90F7MQ8 (Pregnenolone)
[Em] Mês de entrada:1504
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131122
[St] Status:MEDLINE
[do] DOI:10.1055/s-0033-1358739



página 1 de 33 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde