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[PMID]:29244855
[Au] Autor:Glatz W; Steinwender G; Tarmann L; Malle EM; Schörkhuber M; Wackernagel W; Petrovski G; Wedrich A; Ivastinovic D
[Ad] Endereço:Department of Ophthalmology, Medical University of Graz, Graz, Austria.
[Ti] Título:Vitreous hyper-reflective dots in pseudophakic cystoid macular edema assessed with optical coherence tomography.
[So] Source:PLoS One;12(12):e0189194, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: This study compares the presence of vitreous hyper-reflective dots (VHDs) detected with optical coherence tomography (OCT) between eyes with pseudophakic cystoid macular edema (CME) and those with no CME after cataract surgery. In addition, we evaluated the impact of VHDs on the responsiveness of pseudophakic CME to cortisone treatment. SETTING: Department of Ophthalmology, Medical University of Graz, Austria. DESIGN: Retrospective, monocenter case-controlled study. METHODS: Inclusion criteria for the study group and the control group were CME and no CME within 12 weeks following uneventful phacoemulsification in otherwise healthy eyes, respectively. VHDs (number and size) and the macular thickness were assessed with OCT. Furthermore, the number of peribulbar or intravitreal steroid injections was assessed. RESULTS: A total of 284 eyes from 267 patients were analyzed, among which 119 met the inclusion criteria for the study (n = 63) and the control group (n = 56). VHDs were observed in 54 (85.7%) study eyes and 21 (37.5%, p = 0.013) control eyes. The number of VHDs was 3.9±3.4 in the study group and 0.7±1 in the control group (p<0.001). The size of the VHDs was 33.5±9.1 µm and 36.6±17.9 µm in the study and control groups, respectively (p = 0.978). Overall, the number of VHDs correlated with central subfield thickness (r = 0.584, p<0.001), cube volume (r = 0.525, p<0.001), and postoperative best-corrected visual acuity (BCVA) (r = -0.563, p<0.001). The number of VHDs did not correlate with the frequency of peribulbar or intravitreal steroid injections. CONCLUSION: VHDs occurred more often in eyes with CME than in eyes without CME following cataract surgery. In addition, the number of VHDs had an impact on the extent of macular thickening and subsequently postoperative BCVA. No correlation was found between the number of VHDs and the frequency of required peribulbar or intravitreal steroid injections.
[Mh] Termos MeSH primário: Edema Macular/diagnóstico por imagem
Facoemulsificação
Pseudofacia/diagnóstico por imagem
Corpo Vítreo/diagnóstico por imagem
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Anti-Inflamatórios/uso terapêutico
Estudos de Casos e Controles
Catarata/diagnóstico por imagem
Catarata/fisiopatologia
Cortisona/uso terapêutico
Feminino
Seres Humanos
Injeções Intravítreas
Edema Macular/tratamento farmacológico
Edema Macular/fisiopatologia
Edema Macular/cirurgia
Masculino
Meia-Idade
Período Pós-Operatório
Pseudofacia/tratamento farmacológico
Pseudofacia/fisiopatologia
Pseudofacia/cirurgia
Estudos Retrospectivos
Tomografia de Coerência Óptica
Acuidade Visual/fisiologia
Corpo Vítreo/efeitos dos fármacos
Corpo Vítreo/cirurgia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); V27W9254FZ (Cortisone)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189194


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[PMID]:29180003
[Au] Autor:Zhang Q; Chen Z; Chen S; Yu T; Wang J; Wang W; Deng H
[Ad] Endereço:Key Laboratory of Child Development and Learning Science (Southeast University), Ministry of Education, Institute of Child Development and Education, Southeast University, Nanjing 210096, China; School of early childhood education, Jiangsu Second Normal University, Nanjing 210012, China.
[Ti] Título:Correlations of hair level with salivary level in cortisol and cortisone.
[So] Source:Life Sci;193:57-63, 2018 Jan 15.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:AIMS: Contrary findings exist on the consistency between hair cortisol and salivary cortisol in assessing the basal activity of the hypothalamic-pituitary-adrenal (HPA) axis. The mismatches in temporal characteristic and the indices of hair and salivary cortisol might be potential reasons for the inconsistency. The aim of this study was to investigate the consistency between hair and salivary levels in cortisol and cortisone by directly examining the correlation between hair level and salivary level with different temporal characteristics (acute, short-term and long-term levels) and reflecting different HPA functions (basal level and reactivity level) in the well-matched time span. MAIN METHODS: A longitudinal design within a five-week period was conducted in a sample of 44 healthy female college students (mean age: 18.8yrs.; age range: 18-22yrs) of Han nationality with the exclusion criteria, such as use of oral contraceptives or glucocorticoids and bleached hairs, etc. Four saliva samples (awakening, awakening+30min, awakening+4h and awakening+9h) were collected from an identical participant on three separate days with an interval of one week and 1-cm hair segment nearest to the scalp was collected two weeks later after completing saliva collection. Cortisol and cortisone in saliva and hair were simultaneously measured with high performance liquid chromatography tandem mass spectrometry. KEY FINDINGS: There were significantly moderate correlations in cortisol and cortisone between hair level and three-day average of single-day salivary level, but low to moderate correlations between hair level and single-point and single-day salivary level. Hair cortisol and cortisone were unrelated to single-day level and three-day average of diurnal slope and cortisol awakening response of salivary cortisol and cortisone, respectively. SIGNIFICANCE: The considerable consistency between hair level and long-term salivary level in cortisol and cortisone implies that cortisol and cortisone in hair are valid biomarkers of cumulative exposure of cortisol and cortisone to retrospectively reflect long-term basal activity of the HPA system.
[Mh] Termos MeSH primário: Cortisona/análise
Hidrocortisona/análise
[Mh] Termos MeSH secundário: Adolescente
China
Cromatografia Líquida de Alta Pressão
Ritmo Circadiano/fisiologia
Cortisona/química
Grupos Étnicos
Feminino
Cabelo/química
Seres Humanos
Hidrocortisona/química
Sistema Hipotálamo-Hipofisário
Estudos Longitudinais
Sistema Hipófise-Suprarrenal
Saliva/química
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
V27W9254FZ (Cortisone); WI4X0X7BPJ (Hydrocortisone)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE


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[PMID]:29182112
[Au] Autor:Rapp M; Grauel F; Wessel LM; Illing P; Kaiser MM
[Ti] Título:Treatment outcome in 60 children with pathological fractures of the humerus caused by juvenile or aneurysmal bone cysts.
[So] Source:Acta Orthop Belg;82(4):723-729, 2016 Dec.
[Is] ISSN:0001-6462
[Cp] País de publicação:Belgium
[La] Idioma:eng
[Ab] Resumo:The treatment of pathological fractures of the humerus caused by juvenile or aneurysmal bone cysts (JBC/ABC) should be a single approach with a high success rate and low complication rate. This study evaluates how day by day treatment concepts fulfil these aims. Children below 15 years of age with a pathological fracture of the humerus caused by a JBC or ABC between 01.01.2001 and 31.12.2010, were investigated by chart review in four major paediatric trauma centres. Age, gender, fracture localisation, X-ray findings, treatment and outcome - assessed by the Capanna classification (I to IV), were analysed. 60 children [41male, 19 female; mean age: 9 years (4-14 years)] with 43 JBC and 12 ABC were included as well as five cysts, who could not be classified definitively. First treatment was non-operatively in 33 children. Of these 27 cysts did not improve; likewise the supportive installation of cortisone in six patients did not change the outcome. The first treatment consisted of elastic stable intramedullary in 13 children; up to three nail exchanges included. But only six of these reached (nearly) complete resolution (I/II). Overall the combined mechanical and biological treatment with curettage, elastic stable intramedullary nailing, (artificial) bone substitute and in some cases growth factors was performed as the 1st-line treatment in nine patients and further in 2nd or 3rd-line treatments in 13 humeral cysts. More than half of these reached a complete or nearly complete resolution of the cyst (12x I, 5x II, 1x III, 4x IV). Major complications in all operated patients were six nails not removable and two children with upper extremities length differences. Healing rates are low for non-operative treatment, elastic stable intramedullary nailing alone and by using cortisone for cysts resolution in pathological fractures of the humerus. Data support a combined mechanical and biological treatment with curettage, elastic stable intramedullary nailing, (artificial) bone substitute and the use of growth factors.
[Mh] Termos MeSH primário: Anti-Inflamatórios/uso terapêutico
Cistos Ósseos Aneurismáticos/terapia
Substitutos Ósseos/uso terapêutico
Cortisona/uso terapêutico
Fixação Intramedular de Fraturas/métodos
Fraturas Espontâneas/terapia
Fraturas do Úmero/terapia
[Mh] Termos MeSH secundário: Adolescente
Cistos Ósseos/complicações
Cistos Ósseos/diagnóstico por imagem
Cistos Ósseos/terapia
Cistos Ósseos Aneurismáticos/complicações
Cistos Ósseos Aneurismáticos/diagnóstico por imagem
Pinos Ortopédicos
Criança
Pré-Escolar
Tratamento Conservador
Feminino
Consolidação da Fratura
Fraturas Espontâneas/diagnóstico por imagem
Fraturas Espontâneas/etiologia
Seres Humanos
Fraturas do Úmero/diagnóstico por imagem
Fraturas do Úmero/etiologia
Injeções Intralesionais
Masculino
Radiografia
Estudos Retrospectivos
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Bone Substitutes); V27W9254FZ (Cortisone)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171228
[Lr] Data última revisão:
171228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE


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[PMID]:28457967
[Au] Autor:Araya S; Kratschmar DV; Tsachaki M; Stücheli S; Beck KR; Odermatt A
[Ad] Endereço:Division of Molecular and Systems Toxicology, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50, 4056 Basel, Switzerland.
[Ti] Título:DHRS7 (SDR34C1) - A new player in the regulation of androgen receptor function by inactivation of 5α-dihydrotestosterone?
[So] Source:J Steroid Biochem Mol Biol;171:288-295, 2017 07.
[Is] ISSN:1879-1220
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:DHRS7 (SDR34C1) has been associated with potential tumor suppressor effects in prostate cancer; however, its function remains largely unknown. Recent experiments using purified recombinant human DHRS7 suggested several potential substrates, including the steroids cortisone and Δ4-androstene-3,17-dione (androstenedione). However, the substrate and cofactor concentrations used in these experiments were very high and the physiological relevance of these observations needed to be further investigated. In the present study, recombinant human DHRS7 was expressed in intact HEK-293 cells in order to investigate whether glucocorticoids and androgens serve as substrates at sub-micromolar concentrations and at physiological cofactor concentrations. Furthermore, the membrane topology of DHRS7 was revisited using redox-sensitive green-fluorescent protein fusions in living cells. The results revealed that (1) cortisone is a substrate of DHRS7; however, it is not reduced to cortisol but to 20ß-dihydrocortisone, (2) androstenedione is not a relevant substrate of DHRS7, (3) DHRS7 catalyzes the oxoreduction of 5α-dihydrotestosterone (5αDHT) to 3α-androstanediol (3αAdiol), with a suppressive effect on androgen receptor (AR) transcriptional activity, and (4) DHRS7 is anchored in the endoplasmic reticulum membrane with a cytoplasmic orientation. Together, the results show that DHRS7 is a cytoplasmic oriented enzyme exhibiting 3α/20ß-hydroxysteroid dehydrogenase activity, with a possible role in the modulation of AR function. Further research needs to address the physiological relevance of DHRS7 in the inactivation of 5αDHT and AR regulation.
[Mh] Termos MeSH primário: Androgênios/metabolismo
Di-Hidrotestosterona/metabolismo
Regulação para Baixo
Retículo Endoplasmático/enzimologia
Oxirredutases/metabolismo
Receptores Androgênicos/metabolismo
[Mh] Termos MeSH secundário: Androgênios/química
Androstano-3,17-diol/química
Androstano-3,17-diol/metabolismo
Cortisona/análogos & derivados
Cortisona/química
Cortisona/metabolismo
Di-Hidrotestosterona/química
Glucocorticoides/metabolismo
Proteínas de Fluorescência Verde/genética
Proteínas de Fluorescência Verde/metabolismo
Células HEK293
Seres Humanos
Ligantes
Conformação Molecular
Oligopeptídeos/genética
Oligopeptídeos/metabolismo
Concentração Osmolar
Oxirredução
Oxirredutases/química
Oxirredutases/genética
Fragmentos de Peptídeos/química
Fragmentos de Peptídeos/genética
Fragmentos de Peptídeos/metabolismo
Transporte Proteico
Receptores Androgênicos/química
Receptores Androgênicos/genética
Proteínas Recombinantes de Fusão/metabolismo
Proteínas Recombinantes/metabolismo
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (AR protein, human); 0 (Androgens); 0 (Glucocorticoids); 0 (Ligands); 0 (Oligopeptides); 0 (Peptide Fragments); 0 (Receptors, Androgen); 0 (Recombinant Fusion Proteins); 0 (Recombinant Proteins); 08J2K08A3Y (Dihydrotestosterone); 147336-22-9 (Green Fluorescent Proteins); 25126-76-5 (Androstane-3,17-diol); 3615-87-0 (4-pregnene-17 alpha,20 beta,21-triol-3,11-dione); 98849-88-8 (FLAG peptide); EC 1.- (DHRS7 protein, human); EC 1.- (Oxidoreductases); V27W9254FZ (Cortisone)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171208
[Lr] Data última revisão:
171208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


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[PMID]:28911138
[Au] Autor:Debono M; Harrison RF; Chadarevian R; Gueroult C; Abitbol JL; Newell-Price J
[Ad] Endereço:Academic Unit of Endocrinology, University of Sheffield, Sheffield S10 2TN, United Kingdom.
[Ti] Título:Resetting the Abnormal Circadian Cortisol Rhythm in Adrenal Incidentaloma Patients With Mild Autonomous Cortisol Secretion.
[So] Source:J Clin Endocrinol Metab;102(9):3461-3469, 2017 Sep 01.
[Is] ISSN:1945-7197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Context: Adrenal incidentalomas (AIs) are found commonly on axial imaging. Around 30% exhibit autonomous cortisol secretion (ACS) associated with increased cardiovascular events and death. Objective: We hypothesized that AI/ACS patients have an abnormal cortisol rhythm that could be reversed by use of carefully timed short-acting cortisol synthesis blockade, with improvement in cardiovascular disease markers. Design, Setting, and Participants: In a phase 1/2a, prospective study (Eudract no. 2012-002586-35), we recruited six patients with AI/ACS and two control groups of six sex-, age-, and body mass index-matched individuals: (1) patients with AI and no ACS (AI/NoACS) and (2) healthy volunteers with no AI [healthy controls (HC)]. Twenty-four-hour circadian cortisol analysis was performed to determine any differences between groups and timing of intervention for cortisol lowering using the 11ß-hydroxylase inhibitor metyrapone. Circadian profiles of serum interleukin-6 (IL-6) were assessed. Results: Serum cortisol levels in group AI/ACS were significantly higher than both group AI/NoACS and group HC from 6 pm to 10 pm [area under the curve (AUC) difference: 0.81 nmol/L/h; P = 0.01] and from 10 pm to 2 am (AUC difference: 0.86 nmol/L/h; P < 0.001). In light of these findings, patients with ACS received metyrapone 500 mg at 6 pm and 250 mg at 10 pm, and cortisol rhythms were reassessed. Postintervention evening serum cortisol was lowered, similar to controls [6 pm to 10 pm (AUC difference: -0.06 nmol/L/h; P = 0.85); 10 pm to 2 am (AUC difference: 0.10 nmol/L/h; P = 0.76)]. Salivary cortisone showed analogous changes. IL-6 levels were elevated before treatment [10 pm to 2 pm (AUC difference: 0.42 pg/mL/h; P = 0.01)] and normalized post treatment. Conclusions: In AI/ACS, the evening and nocturnal cortisol exposure is increased. Use of timed evening doses of metyrapone resets the cortisol rhythm to normal. This unique treatment paradigm is associated with a reduction in the cardiovascular risk marker IL-6.
[Mh] Termos MeSH primário: Neoplasias das Glândulas Suprarrenais/metabolismo
Ritmo Circadiano
Hidrocortisona/secreção
Metirapona/administração & dosagem
[Mh] Termos MeSH secundário: Neoplasias das Glândulas Suprarrenais/tratamento farmacológico
Neoplasias das Glândulas Suprarrenais/patologia
Idoso
Área Sob a Curva
Índice de Massa Corporal
Estudos de Casos e Controles
Cortisona/sangue
Cortisona/secreção
Relação Dose-Resposta a Droga
Esquema de Medicação
Feminino
Seres Humanos
Hidrocortisona/sangue
Interleucina-6/metabolismo
Masculino
Meia-Idade
Estudos Prospectivos
Estatísticas não Paramétricas
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Interleukin-6); V27W9254FZ (Cortisone); WI4X0X7BPJ (Hydrocortisone); ZS9KD92H6V (Metyrapone)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE
[do] DOI:10.1210/jc.2017-00823


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[PMID]:28808144
[Au] Autor:Li H; Cai J; Chen R; Zhao Z; Ying Z; Wang L; Chen J; Hao K; Kinney PL; Chen H; Kan H
[Ad] Endereço:From School of Public Health, Key Lab of Public Health Safety of the Ministry of Education and Key Lab of Health Technology Assessment of the Ministry of Health, Fudan University, Shanghai, China (H.L., J.C., R.C., Z.Z., Z.Y., H.K.); Shanghai Key Laboratory of Atmospheric Particle Pollution and Prev
[Ti] Título:Particulate Matter Exposure and Stress Hormone Levels: A Randomized, Double-Blind, Crossover Trial of Air Purification.
[So] Source:Circulation;136(7):618-627, 2017 Aug 15.
[Is] ISSN:1524-4539
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Exposure to ambient particulate matter (PM) is associated with a number of adverse health outcomes, but potential mechanisms are largely unknown. Metabolomics represents a powerful approach to study global metabolic changes in response to environmental exposures. We therefore conducted this study to investigate changes in serum metabolites in response to the reduction of PM exposure among healthy college students. METHODS: We conducted a randomized, double-blind crossover trial in 55 healthy college students in Shanghai, China. Real and sham air purifiers were placed in participants' dormitories in random order for 9 days with a 12-day washout period. Serum metabolites were quantified by using gas chromatography-mass spectrometry and ultrahigh performance liquid chromatography-mass spectrometry. Between-treatment differences in metabolites were examined using orthogonal partial least square-discriminant analysis and mixed-effect models. Secondary outcomes include blood pressure, corticotropin-releasing hormone, adrenocorticotropic hormone, insulin resistance, and biomarkers of oxidative stress and inflammation. RESULTS: The average personal exposure to PMs with aerodynamic diameters ≤2.5 µm was 24.3 µg/m during the real purification and 53.1 µg/m during the sham purification. Metabolomics analysis showed that higher exposure to PMs with aerodynamic diameters ≤2.5 µm led to significant increases in cortisol, cortisone, epinephrine, and norepinephrine. Between-treatment differences were also observed for glucose, amino acids, fatty acids, and lipids. We found significantly higher blood pressure, hormones, insulin resistance, and biomarkers of oxidative stress and inflammation among individuals exposed to higher PMs with aerodynamic diameters ≤2.5 µm. CONCLUSIONS: This study suggests that higher PM may induce metabolic alterations that are consistent with activations of the hypothalamus-pituitary-adrenal and sympathetic-adrenal-medullary axes, adding potential mechanistic insights into the adverse health outcomes associated with PM. Furthermore, our study demonstrated short-term reductions in stress hormone following indoor air purification. CLINICAL TRIAL REGISTRATION: URL: http://www.clinicaltrials.gov. Unique identifier: NCT02712333.
[Mh] Termos MeSH primário: Filtros de Ar
Hormônios/sangue
Estresse Oxidativo/efeitos dos fármacos
Material Particulado/toxicidade
[Mh] Termos MeSH secundário: Biomarcadores/sangue
China
Cromatografia Líquida de Alta Pressão
Cortisona/sangue
Estudos Cross-Over
Método Duplo-Cego
Exposição Ambiental
Epinefrina/sangue
Feminino
Cromatografia Gasosa-Espectrometria de Massas
Seres Humanos
Hidrocortisona/sangue
Hipertensão/etiologia
Metabolismo dos Lipídeos/efeitos dos fármacos
Masculino
Metabolômica
Material Particulado/análise
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Biomarkers); 0 (Hormones); 0 (Particulate Matter); V27W9254FZ (Cortisone); WI4X0X7BPJ (Hydrocortisone); YKH834O4BH (Epinephrine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170816
[St] Status:MEDLINE
[do] DOI:10.1161/CIRCULATIONAHA.116.026796


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[PMID]:28797028
[Au] Autor:Cirillo N; Morgan DJ; Pedicillo MC; Celentano A; Lo Muzio L; McCullough MJ; Prime SS
[Ad] Endereço:Melbourne Dental School, The University of Melbourne, 720 Swanston Street, Carlton, Melbourne, VIC 3053, Australia.
[Ti] Título:Characterisation of the cancer-associated glucocorticoid system: key role of 11ß-hydroxysteroid dehydrogenase type 2.
[So] Source:Br J Cancer;117(7):984-993, 2017 Sep 26.
[Is] ISSN:1532-1827
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Recent studies have shown that production of cortisol not only takes place in several non-adrenal peripheral tissues such as epithelial cells but, also, the local inter-conversion between cortisone and cortisol is regulated by the 11ß-hydroxysteroid dehydrogenases (11ß-HSDs). However, little is known about the activity of this non-adrenal glucocorticoid system in cancers. METHODS: The presence of a functioning glucocorticoid system was assessed in human skin squamous cell carcinoma (SCC) and melanoma and further, in 16 epithelial cell lines from 8 different tissue types using ELISA, western blotting and immunofluorescence. 11ß-HSD2 was inhibited both pharmacologically and by siRNA technology. Naïve CD8 T cells were used to test the paracrine effects of cancer-derived cortisol on the immune system in vitro. Functional assays included cell-cell adhesion and cohesion in two- and three-dimensional models. Immunohistochemical data of 11ß-HSD expression were generated using tissue microarrays of 40 cases of human SCCs as well as a database featuring 315 cancer cases from 15 different tissues. RESULTS: We show that cortisol production is a common feature of malignant cells and has paracrine functions. Cortisol production correlated with the magnitude of glucocorticoid receptor (GR)-dependent inhibition of tumour-specific CD8 T cells in vitro. 11ß-HSDs were detectable in human skin SCCs and melanoma. Analyses of publicly available protein expression data of 11ß-HSDs demonstrated that 11ß-HSD1 and -HSD2 were dysregulated in the majority (73%) of malignancies. Pharmacological manipulation of 11ß-HSD2 activity by 18ß-glycyrrhetinic acid (GA) and silencing by specific siRNAs modulated the bioavailability of cortisol. Cortisol also acted in an autocrine manner and promoted cell invasion in vitro and cell-cell adhesion and cohesion in two- and three-dimensional models. Immunohistochemical analyses using tissue microarrays showed that expression of 11ß-HSD2 was significantly reduced in human SCCs of the skin. CONCLUSIONS: The results demonstrate evidence of a cancer-associated glucocorticoid system and show for the first time, the functional significance of cancer-derived cortisol in tumour progression.
[Mh] Termos MeSH primário: 11-beta-Hidroxiesteroide Desidrogenase Tipo 2/metabolismo
Carcinoma de Células Escamosas/enzimologia
Células Epiteliais/enzimologia
Hidrocortisona/metabolismo
Melanoma/enzimologia
Neoplasias Cutâneas/enzimologia
[Mh] Termos MeSH secundário: 11-beta-Hidroxiesteroide Desidrogenase Tipo 1/metabolismo
11-beta-Hidroxiesteroide Desidrogenase Tipo 2/análise
11-beta-Hidroxiesteroide Desidrogenase Tipo 2/antagonistas & inibidores
11-beta-Hidroxiesteroide Desidrogenase Tipo 2/genética
Hormônio Adrenocorticotrópico/farmacologia
Linfócitos T CD8-Positivos/efeitos dos fármacos
Linfócitos T CD8-Positivos/imunologia
Carcinoma de Células Escamosas/química
Adesão Celular
Proliferação Celular/efeitos dos fármacos
Cortisona/farmacologia
Meios de Cultivo Condicionados/farmacologia
Regulação para Baixo
Inativação Gênica
Ácido Glicirretínico/análogos & derivados
Ácido Glicirretínico/farmacologia
Células HT29
Seres Humanos
Hidrocortisona/imunologia
Hidrocortisona/farmacologia
Queratinócitos/efeitos dos fármacos
Queratinócitos/metabolismo
Células MCF-7
Melanoma/química
Comunicação Parácrina
Receptores de Glucocorticoides/imunologia
Receptores de Glucocorticoides/metabolismo
Neoplasias Cutâneas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media, Conditioned); 0 (Receptors, Glucocorticoid); 1449-05-4 (18alpha-glycyrrhetinic acid); 9002-60-2 (Adrenocorticotropic Hormone); EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenase Type 1); EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenase Type 2); P540XA09DR (Glycyrrhetinic Acid); V27W9254FZ (Cortisone); WI4X0X7BPJ (Hydrocortisone)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1038/bjc.2017.243


  8 / 7607 MEDLINE  
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[PMID]:28735224
[Au] Autor:Li C; Zhang Z; Liu X; Shen K; Gu P; Kang X
[Ad] Endereço:Key Laboratory of Child Development and Learning Science (Ministry of Education), School of Biological Science & Medical Engineering, Southeast University, Nanjing 210096, China. Electronic address: chenlee1203@163.com.
[Ti] Título:Simultaneous quantification of cortisol and cortisone in urines from infants with packed-fiber solid-phase extraction coupled to HPLC-MS/MS.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1061-1062:163-168, 2017 Sep 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Cortisol and cortisone are two important glucocorticoids in human body, their interconversion is controlled by two isotypes of 11ß-hydroxy steroid dehydrogenase (11ß-HSD1 and 11ß-HSD2). The ratio of urinary cortisol to cortisone can be used to assess the activity of 11ß-HSDs. An analytical method to quantify urinary cortisol and cortisone using high performance liquid chromatographic tandem mass spectrometry following a packed-fiber solid-phase extraction (PFSPE) was developed. The proposed method was validated and applied to determine the urinary cortisol and cortisone concentrations in infants. Linearity was observed in the range of 0.6-150ng/mL for cortisol and 0.8-200ng/mL for cortisone. The intra-day RSD was 2.4-4.5% for cortisol and 3.3-6.2% for cortisone. Inter-day RSD was 3.7-6.6% for cortisol and 4.3-8.2% for cortisone. The recovery was 97.8±4.6% for cortisol and 98.9±4.4% for cortisone. The established method is simple and efficient for the quantification of urinary cortisol and cortisone and for indirectly assessing the activity of 11ß-hydroxy steroid dehydrogenase.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Cortisona/urina
Hidrocortisona/urina
Extração em Fase Sólida/métodos
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Cortisona/isolamento & purificação
Seres Humanos
Hidrocortisona/isolamento & purificação
Lactente
Limite de Detecção
Modelos Lineares
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
V27W9254FZ (Cortisone); WI4X0X7BPJ (Hydrocortisone)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170724
[St] Status:MEDLINE


  9 / 7607 MEDLINE  
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[PMID]:28505491
[Au] Autor:Matsumoto A; Shimanoe C; Tanaka K; Ichiba M; Hara M
[Ad] Endereço:Department of Social Medicine, Saga University School of Medicine, Saga 849-8501, Japan.
[Ti] Título:Development of suitable method for large-scale urinary glucocorticoid analysis by liquid chromatography-mass spectrometry.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1057:62-69, 2017 Jul 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Levels of urinary glucocorticoids and their concentration ratios have been analyzed as potential markers for various pathological statuses. Large-scale studies may possibly accelerate the investigations; however, a suitable method needs to be established. Analytical conditions for measurement of urinary glucocorticoids with LCMS were examined. Electrospray ionization in the positive ion mode was applied for detection of cortisol (precursor>product ion: 363.3>121.0), cortisol-d4 (internal standard, IS, 367.4>121.1), and cortisone (361.2>163.2). To maximize ionization, acetic acid-ammonium acetate buffer (18mM) at pH 5.3 was employed as eluent A. A C18 column (100mm×2.1mm, 2.7µm) at 50°C was used for the 9.5min binary gradient separation starting with 60% eluent A with methanol being eluent B. Linear correlations were observed between the concentrations and the peak areas in the concentration range of 1-300ng/mL with correlation coefficients (r) of 0.998 and 0.997 for cortisol and cortisone, respectively, without IS adjustment, and 0.999 with IS adjustment for both cortisol and cortisone. Solid-phase extraction (SPE) using a 2mL centrifuge column was performed for the urine samples, with the original and final volumes being 100µL. The SPE of 12 urine specimens could be performed within 30min. The effect of the sample matrix on the quantification of endogenous compounds present in the urine extract was limited (coefficient of variation (CV) of IS-adjusted matrix factor: 4.4-8.1%; urine extracts of 8 individuals); however, substantial peak reduction of cortisol was observed at low concentrations. Exogenous contaminants originating from the SPE centrifuge column seemed to be a main cause for this phenomenon because the pure-water extract showed similar peak reduction. A recovery of ∼50% was obtained for both cortisol and cortisone. Adjustment with the IS improved the apparent recovery, with ∼100% being obtained for both cortisol and cortisone. The recovery rate decreased when the urine samples were concentrated in the SPE step; the reduction was greater for cortisol than for cortisone. The lower limit of quantification (LLOQ) was set at 2.5ng/mL when the injection volume was 10µL, based on the reproducibility of the standards which were measured (CV of 12 repetitions: 10.1% for 0.5ng/mL cortisol and 19.6% for 1ng/mL cortisone), the matrix effect (-55% at 2ng/mL concentrations of cortisol), and the recovery rate (∼50%). Furthermore an alternative approach for preparation of the cortisol standards was required for low concentration range (2.5-20ng/mL) because of the effect of the matrix. Degradation of original urine specimens at room temperature was minimal during the first 24h. The extracted urine samples degraded over time; however, their concentrations were corrected with the IS, allowing for analysis up to 5days after extraction. In conclusion, an analytical method for urinary glucocorticoids was established, which is fast, sensitive, and well suited for practical application to large-scale study.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão
Cortisona/urina
Glucocorticoides/urina
Hidrocortisona/urina
Espectrometria de Massas
[Mh] Termos MeSH secundário: Biomarcadores/análise
Biomarcadores/urina
Calibragem
Seres Humanos
Sensibilidade e Especificidade
Extração em Fase Sólida
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Glucocorticoids); V27W9254FZ (Cortisone); WI4X0X7BPJ (Hydrocortisone)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170516
[St] Status:MEDLINE


  10 / 7607 MEDLINE  
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[PMID]:28363270
[Ti] Título:Hip Pain: Dry Needling Versus Cortisone Injections.
[So] Source:J Orthop Sports Phys Ther;47(4):240, 2017 Apr.
[Is] ISSN:1938-1344
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Greater trochanteric pain syndrome (GTPS) is chronic, intermittent pain and tenderness on the outside of the hip. The medical community once thought that a swollen hip bursa was the source of such pain, which led to the use of corticosteroid injections to the bursa to help decrease swelling and pain. However, researchers now believe that injuries to the muscles and tendons around the hip are the actual cause of this pain, and that inflammation is often not involved. A study published in the April 2017 issue of JOSPT explores dry needling as an alternative to cortisone injections to reduce pain and improve function in patients with GTPS. J Orthop Sports Phys Ther 2017;47(4):240. doi:10.2519/jospt.2017.0504.
[Mh] Termos MeSH primário: Artralgia/terapia
Cortisona/administração & dosagem
Glucocorticoides/administração & dosagem
Articulação do Quadril
Modalidades de Fisioterapia
[Mh] Termos MeSH secundário: Bursite/terapia
Dor Crônica
Fêmur
Seres Humanos
Injeções Intra-Articulares
Agulhas
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Glucocorticoids); V27W9254FZ (Cortisone)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170402
[St] Status:MEDLINE
[do] DOI:10.2519/jospt.2017.0504



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