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[PMID]:29358154
[Au] Autor:Turner AD; Waack J; Lewis A; Edwards C; Lawton L
[Ad] Endereço:Centre for Environment, Fisheries and Aquaculture Science, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, United Kingdom. Electronic address: andrew.turner@cefas.co.uk.
[Ti] Título:Development and single-laboratory validation of a UHPLC-MS/MS method for quantitation of microcystins and nodularin in natural water, cyanobacteria, shellfish and algal supplement tablet powders.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1074-1075:111-123, 2018 Feb 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A simple, rapid UHPLC-MS/MS method has been developed and optimised for the quantitation of microcystins and nodularin in wide variety of sample matrices. Microcystin analogues targeted were MC-LR, MC-RR, MC-LA, MC-LY, MC-LF, LC-LW, MC-YR, MC-WR, [Asp3] MC-LR, [Dha7] MC-LR, MC-HilR and MC-HtyR. Optimisation studies were conducted to develop a simple, quick and efficient extraction protocol without the need for complex pre-analysis concentration procedures, together with a rapid sub 5min chromatographic separation of toxins in shellfish and algal supplement tablet powders, as well as water and cyanobacterial bloom samples. Validation studies were undertaken on each matrix-analyte combination to the full method performance characteristics following international guidelines. The method was found to be specific and linear over the full calibration range. Method sensitivity in terms of limits of detection, quantitation and reporting were found to be significantly improved in comparison to LC-UV methods and applicable to the analysis of each of the four matrices. Overall, acceptable recoveries were determined for each of the matrices studied, with associated precision and within-laboratory reproducibility well within expected guidance limits. Results from the formalised ruggedness analysis of all available cyanotoxins, showed that the method was robust for all parameters investigated. The results presented here show that the optimised LC-MS/MS method for cyanotoxins is fit for the purpose of detection and quantitation of a range of microcystins and nodularin in shellfish, algal supplement tablet powder, water and cyanobacteria. The method provides a valuable early warning tool for the rapid, routine extraction and analysis of natural waters, cyanobacterial blooms, algal powders, food supplements and shellfish tissues, enabling monitoring labs to supplement traditional microscopy techniques and report toxicity results within a short timeframe of sample receipt. The new method, now accredited to ISO17025 standard, is simple, quick, applicable to multiple matrices and is highly suitable for use as a routine, high-throughout, fast turnaround regulatory monitoring tool.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Cianobactérias/química
Suplementos Nutricionais/análise
Microcistinas/análise
Peptídeos Cíclicos/análise
Frutos do Mar/análise
[Mh] Termos MeSH secundário: Animais
Produtos Biológicos/análise
Produtos Biológicos/química
Bivalves
Contaminação de Alimentos
Limite de Detecção
Modelos Lineares
Reprodutibilidade dos Testes
Comprimidos
Espectrometria de Massas em Tandem/métodos
Poluentes Químicos da Água/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biological Products); 0 (Microcystins); 0 (Peptides, Cyclic); 0 (Tablets); 0 (Water Pollutants, Chemical); 0979BIK2QU (nodularin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180124
[St] Status:MEDLINE


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[PMID]:29248874
[Au] Autor:Ma J; Li Y; Wu M; Li X
[Ad] Endereço:College of Life Science, Henan Normal University, Xinxiang, Henan 453007, China.
[Ti] Título:Oxidative stress-mediated p53/p21 pathway may be involved in microcystin-LR-induced cytotoxicity in HepG2 cells.
[So] Source:Chemosphere;194:773-783, 2018 Mar.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A previous study showed that microcystin-LR (MC-LR) exerted cytotoxicity and induced apoptosis in HepG2 cells. In the present study, we investigated whether oxidative stress-mediated p53/p21 is involved in this process to further elucidate the mechanism of cytotoxicity induced by MC-LR. Morphological evaluation showed that MC-LR induced time- and dose-dependent cytotoxicity in HepG2 cells. Biochemical assays revealed that MC-LR exposure altered the protein levels of HSP70 and HSP90, generally inhibited superoxide dismutase and catalase, reduced glutathione content, and increased the cellular malondialdehyde level of HepG2 cells, suggesting that MC-LR may induce biochemical disturbance and oxidative stress in HepG2 cells. The protein levels of p-p53 and p21 were markedly increased by MC-LR exposure in a concentration-dependent manner, suggesting that p53 and p21 may be involved in the process. Moreover, we also found that the proto-oncogene c-myc was significantly activated in HepG2 cells following MC-LR exposure, indicating that c-myc in HepG2 cells was potentially involved in response to MC-LR-induced apoptosis. These findings may contribute to further understanding the in vitro molecular mechanism of MC-LR hepatotoxicity.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Inibidor de Quinase Dependente de Ciclina p21/metabolismo
Microcistinas/farmacologia
Estresse Oxidativo/efeitos dos fármacos
Proteína Supressora de Tumor p53/metabolismo
[Mh] Termos MeSH secundário: Catalase/metabolismo
Glutationa/metabolismo
Células Hep G2
Seres Humanos
Malondialdeído/metabolismo
Proteínas Proto-Oncogênicas c-myc/metabolismo
Superóxido Dismutase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CDKN1A protein, human); 0 (Cyclin-Dependent Kinase Inhibitor p21); 0 (MYC protein, human); 0 (Microcystins); 0 (Proto-Oncogene Proteins c-myc); 0 (Tumor Suppressor Protein p53); 4Y8F71G49Q (Malondialdehyde); EC 1.11.1.6 (Catalase); EC 1.15.1.1 (Superoxide Dismutase); EQ8332842Y (cyanoginosin LR); GAN16C9B8O (Glutathione)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171218
[St] Status:MEDLINE


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[PMID]:29117589
[Au] Autor:Ma J; Li Y; Duan H; Sivakumar R; Li X
[Ad] Endereço:College of Life Science, Henan Normal University, Xinxiang, Henan 453007, China.
[Ti] Título:Chronic exposure of nanomolar MC-LR caused oxidative stress and inflammatory responses in HepG2 cells.
[So] Source:Chemosphere;192:305-317, 2018 Feb.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Low dose but long-term exposure of microcystin-LR (MC-LR) could induce human hepatitis and promote liver cancer according to epidemiological investigation results, but the exact mechanism has not been completely elucidated. In the present study, a chronic toxicity test of MC-LR exposure on HepG2 cells at 0.1-30 nM for 83 d was conducted under laboratory conditions. The western blot assay result revealed that MC-LR entered HepG2 cells, even at the concentration of 0.1 nM, after 83 d of exposure, but no cytotoxicity was observed in the HepG2 cells, as determined by the CCK-8 and LDH tests. However, the results of the DCF fluorescence assay showed that the intracellular ROS level in the 30 nM MC-LR-treated cells was significantly higher than that of the control cells, and 5 and 10 nM of MC-LR exposure totally increased the activity of SOD in HepG2 cells. These results indicate that MC-LR exposure at low concentration also induced excessive ROS in HepG2 cells. Additionally, long-term exposure of MC-LR at low concentration remarkably promoted the expression of NF-κB p65, COX-2, iNOS, TNF-α, IL-1ß, and IL-6 in the cells, suggesting that long-term MC-LR exposure at low concentration can induce inflammatory reaction to HepG2 cells, which might account for MC-induced human hepatitis. Thus, we hypothesized that the pathogenesis of human hepatitis and hepatocarcinoma caused by MCs might be closely associated with oxidative stress and inflammation.
[Mh] Termos MeSH primário: Carcinoma Hepatocelular/patologia
Inflamação/induzido quimicamente
Neoplasias Hepáticas/induzido quimicamente
Microcistinas/toxicidade
Estresse Oxidativo/efeitos dos fármacos
[Mh] Termos MeSH secundário: Toxinas Bacterianas/farmacologia
Carcinoma Hepatocelular/induzido quimicamente
Carcinoma Hepatocelular/metabolismo
Células Hep G2
Hepatite/etiologia
Hepatite/metabolismo
Hepatite/patologia
Seres Humanos
Interleucina-6/metabolismo
Microcistinas/farmacocinética
NF-kappa B/metabolismo
Espécies Reativas de Oxigênio/metabolismo
Fator de Necrose Tumoral alfa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Toxins); 0 (Interleukin-6); 0 (Microcystins); 0 (NF-kappa B); 0 (Reactive Oxygen Species); 0 (Tumor Necrosis Factor-alpha); EQ8332842Y (cyanoginosin LR)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171109
[St] Status:MEDLINE


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[PMID]:29112878
[Au] Autor:Liu G; Ke M; Fan X; Zhang M; Zhu Y; Lu T; Sun L; Qian H
[Ad] Endereço:College of Environment, Zhejiang University of Technology, Hangzhou, 310032, PR China; Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, 310032, PR China.
[Ti] Título:Reproductive and endocrine-disrupting toxicity of Microcystis aeruginosa in female zebrafish.
[So] Source:Chemosphere;192:289-296, 2018 Feb.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Microcystis aeruginosa, a primary species in cyanobacterial blooms, is ubiquitously distributed in water. Microcystins (MCs) purified from M. aeruginosa can exert reproductive toxicity in fish. However, the effects of M. aeruginosa at environmentally relevant levels on the reproductive and endocrine systems of zebrafish are still unknown. The present study investigated the reproductive and endocrine-disrupting toxicity of M. aeruginosa on female zebrafish (Danio rerio) by short-term exposure (96 h). After exposure, marked histological lesions in the liver or gonads, such as nuclear pyknosis and deformation, were observed, and the fertilization rate and hatchability of eggs spawned from treated females were both significantly lower than they were in females in the control group, suggesting the possibility of transgenerational effects of M. aeruginosa exposure. Moreover, M. aeruginosa exposure decreased the concentration of 17ß-estradiol (E2) and testosterone (T) in female zebrafish. Interestingly, the vtg1 transcriptional level significantly decreased in the liver, whereas plasma vitellogenin (VTG) protein levels increased. The present findings indicate that M. aeruginosa could modulate endocrine function by disrupting transcription of hypothalamic-pituitary-gonadal-liver (HPGL) axis-related genes, and impair the reproductive capacity of female zebrafish, suggesting that M. aeruginosa causes potential adverse effects on fish reproduction in Microcystis bloom-contaminated aquatic environments.
[Mh] Termos MeSH primário: Sistema Endócrino/efeitos dos fármacos
Microcistinas/toxicidade
Microcystis/patogenicidade
Reprodução/efeitos dos fármacos
Peixe-Zebra/microbiologia
[Mh] Termos MeSH secundário: Animais
Toxinas Bacterianas/farmacologia
Disruptores Endócrinos/farmacologia
Estradiol/análise
Estradiol/metabolismo
Feminino
Gônadas/efeitos dos fármacos
Gônadas/metabolismo
Gônadas/patologia
Fígado/efeitos dos fármacos
Fígado/metabolismo
Fígado/patologia
Microcistinas/isolamento & purificação
Microcystis/metabolismo
Testosterona/análise
Testosterona/sangue
Vitelogeninas/sangue
Vitelogeninas/metabolismo
Poluentes Químicos da Água/toxicidade
Peixe-Zebra/metabolismo
Peixe-Zebra/fisiologia
Proteínas de Peixe-Zebra/análise
Proteínas de Peixe-Zebra/efeitos dos fármacos
Proteínas de Peixe-Zebra/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Toxins); 0 (Endocrine Disruptors); 0 (Microcystins); 0 (Vitellogenins); 0 (Water Pollutants, Chemical); 0 (Zebrafish Proteins); 3XMK78S47O (Testosterone); 4TI98Z838E (Estradiol); 77238-39-2 (microcystin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171108
[St] Status:MEDLINE


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[PMID]:29100119
[Au] Autor:Dixit F; Barbeau B; Mohseni M
[Ad] Endereço:Department of Chemical and Biological Engineering, University of British Columbia, Vancouver, Canada.
[Ti] Título:Simultaneous uptake of NOM and Microcystin-LR by anion exchange resins: Effect of inorganic ions and resin regeneration.
[So] Source:Chemosphere;192:113-121, 2018 Feb.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study investigated the efficiency of a strongly basic macroporous anion exchange resin for the co-removal of Microcystin-LR (MCLR) and natural organic matter (NOM) in waters affected by toxic algal blooms. Environmental factors influencing the uptake behavior included MCLR and resin concentrations, NOM and anionic species, specifically nitrate, sulphate and bicarbonate. A860 resin exhibited an excellent adsorption capacity of 3800 µg/g; more than 60% of the MCLR removal was achieved within 10 min with a resin dosage of 200 mg/L (∼1 mL/L). Further, kinetic studies revealed that the overall removal of MCLR is influenced by both external diffusion and intra-particle diffusion. Increasing NOM concentration resulted in a significant reduction of MCLR uptake, especially at lower resin dosages, where a competitive uptake between the charged NOM fractions and MCLR was observed due to limited active sites. In addition, MCLR uptake was significantly reduced in the presence of sulphate and nitrate in the water matrix. Moreover, performance of the resin proved to be stable from one regeneration cycle to another. Approximately 80% of MCLR and 50% of dissolved organic carbon (DOC) were recovered in the regenerated brine. Evidences of resin saturation and site reduction were also observed after 2000 bed volumes (BV) of operation. For all the investigated water matrices, a resin dosage of 1000 mg/L (∼4.5 mL/L) was sufficient to lower MCLR concentration from 100 µg/L to below the World Health Organization guideline of 1 µg/L, while simultaneously providing more than 80% NOM removal.
[Mh] Termos MeSH primário: Resinas de Troca de Ânions/química
Poluentes Químicos da Água/química
Purificação da Água/métodos
[Mh] Termos MeSH secundário: Adsorção
Bicarbonatos/química
Cinética
Microcistinas/isolamento & purificação
Nitratos/química
Sulfatos/química
Purificação da Água/instrumentação
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anion Exchange Resins); 0 (Bicarbonates); 0 (Microcystins); 0 (Nitrates); 0 (Sulfates); 0 (Water Pollutants, Chemical); EQ8332842Y (cyanoginosin LR)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180123
[Lr] Data última revisão:
180123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171104
[St] Status:MEDLINE


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[PMID]:28947316
[Au] Autor:Min BH; Ravikumar Y; Lee DH; Choi KS; Kim BM; Rhee JS
[Ad] Endereço:Aquaculture Research Division, National Institute of Fisheries Science, Busan 46083, South Korea.
[Ti] Título:Age-dependent antioxidant responses to the bioconcentration of microcystin-LR in the mysid crustacean, Neomysis awatschensis.
[So] Source:Environ Pollut;232:284-292, 2018 Jan.
[Is] ISSN:1873-6424
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Microcystins (MCs) are naturally occurring algal toxins in the aquatic environment and pose a serious threat to the ecosystem. In general, aquatic populations are structured by organisms of different ages, with varying degrees of biochemical and physiological responses. In this study, juvenile and adult marine mysids (Neomysis awatschensis) were exposed to MC-Leucine Arginine (MC-LR) (0.1, 1, and 10 µg L ) for 7 days, and the bioconcentration dynamics and responses of antioxidant defense system were measured during the exposure and additional depuration periods (7 days). MC-LR bioconcentrated in a dose-dependent manner, from a threshold concentration of 1 µg L in both stages, and the levels reduced gradually during the depuration phase. Bioconcentration patterns of MC-LR were highly age-specific, as juvenile mysids showed peaks during the exposure period, whereas adults exhibited a peak on the first day of depuration. After exposure to 10 µg L concentration, elevated levels of malondialdehyde (MDA) and glutathione (GSH) were observed during the late (days 5 and 7) exposure and early (days 1 and 3) depuration periods in juvenile mysids, while adult mysids showed a peak on day 7 of the exposure period. Age-specific responses were also observed in the enzymatic activities of glutathione S-transferase (GST), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GR). Juvenile mysids showed a significant elevation in all enzymatic activities during the exposure and/or depuration phase upon exposure to 10 µg L MC-LR, but only CAT and SOD enzymes showed significant changes during the exposure and/or depuration periods in adults. Overall, our results indicate the bioconcentration potential of MC-LR and its threshold in the marine mysid, in addition to age-specific MC-LR dynamics and subsequent biochemical responses.
[Mh] Termos MeSH primário: Crustáceos/fisiologia
Microcistinas/metabolismo
Poluentes Químicos da Água/metabolismo
[Mh] Termos MeSH secundário: Animais
Antioxidantes
Catalase/metabolismo
Glutationa/metabolismo
Glutationa Peroxidase/metabolismo
Glutationa Redutase
Glutationa Transferase/metabolismo
Malondialdeído
Microcistinas/toxicidade
Estresse Oxidativo
Superóxido Dismutase/metabolismo
Poluentes Químicos da Água/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Microcystins); 0 (Water Pollutants, Chemical); 4Y8F71G49Q (Malondialdehyde); 77238-39-2 (microcystin); EC 1.11.1.6 (Catalase); EC 1.11.1.9 (Glutathione Peroxidase); EC 1.15.1.1 (Superoxide Dismutase); EC 1.8.1.7 (Glutathione Reductase); EC 2.5.1.18 (Glutathione Transferase); EQ8332842Y (cyanoginosin LR); GAN16C9B8O (Glutathione)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180119
[Lr] Data última revisão:
180119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170927
[St] Status:MEDLINE


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[PMID]:29240841
[Au] Autor:Martin RM; Dearth SP; LeCleir GR; Campagna SR; Fozo EM; Zinser ER; Wilhelm SW
[Ad] Endereço:Department of Microbiology, University of Tennessee, Knoxville, Tennessee, United States of America.
[Ti] Título:Microcystin-LR does not induce alterations to transcriptomic or metabolomic profiles of a model heterotrophic bacterium.
[So] Source:PLoS One;12(12):e0189608, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microcystins are secondary metabolites produced by several freshwater, bloom-forming cyanobacterial species. Microcystin-producing cyanobacteria co-occur with a complex community of heterotrophic bacteria. Though conflicting, studies suggest that microcystins affect the physiology of heterotrophic bacteria by inducing oxidative stress and increasing cell envelope permeability. Based on these observations, we hypothesized that exposure to microcystin should induce differential expression in genes responding to oxidative and envelope stress and trigger shifts in metabolite pools. We tested this hypothesis by exposing Escherichia coli MG1655 to 1 and 10 mg/L microcystin-LR and monitored global changes to gene expression, cellular metabolite pools, and lipid composition using RNA-sequencing and UPLC-MS. Contrary to reported studies, we observed no evidence that microcystin-LR induced oxidative or cell envelope stress in E. coli under the tested conditions. Our results suggest a potential difference in mechanism by which microcystin-LR interacts with heterotrophic bacteria vs. cyanobacteria.
[Mh] Termos MeSH primário: Escherichia coli/efeitos dos fármacos
Metaboloma
Microcistinas/toxicidade
Transcriptoma
[Mh] Termos MeSH secundário: Escherichia coli/genética
Escherichia coli/crescimento & desenvolvimento
Escherichia coli/metabolismo
Proteínas de Escherichia coli/metabolismo
Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos
Genes Bacterianos
Metabolismo dos Lipídeos
Estresse Oxidativo
Análise de Sequência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Escherichia coli Proteins); 0 (Microcystins); EQ8332842Y (cyanoginosin LR)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189608


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[PMID]:28467052
[Au] Autor:Liu W; Wang L; Yang X; Zeng H; Zhang R; Pu C; Zheng C; Tan Y; Luo Y; Feng X; Tian Y; Xiao G; Wang J; Huang Y; Luo J; Feng L; Wang F; Yuan C; Yao Y; Qiu Z; Chen JA; Wu L; Nong Q; Lin H; Shu W
[Ti] Título:Environmental Microcystin Exposure Increases Liver Injury Risk Induced by Hepatitis B Virus Combined with Aflatoxin: A Cross-Sectional Study in Southwest China.
[So] Source:Environ Sci Technol;51(11):6367-6378, 2017 Jun 06.
[Is] ISSN:1520-5851
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Three liver hazards, two confirmed-hepatitis B virus (HBV) and aflatoxin (AFB), and one rarely studied in populations-microcystin (MC), simultaneously exist in tropical and humid areas; however, there are no epidemiological data on their risks in the same population. We conducted a community-based cross-sectional survey among 5493 adults in two rural towns and statistically analyzed the comparative and combinative effects of the three factors after detecting HBsAg and HBV DNA titers, determining estimated daily intakes (EDIs) of AFB1 and MC-LR and testing serum AST and ALT as liver injury markers for each participant. We observed a HBsAg(+) rate of 7.6%, a relatively high AFB1 exposure level (mean EDI = 471.30 ng/d), and a relatively low MC-LR exposure level (mean EDI = 228.25 ng/d). ORs for abnormal AST (2.42, 95%CI = 1.69-3.45) and ALT (2.87, 95%CI = 1.91-4.29) increased in HBV infections compared with HBV-unexposed participants but did not increase in participants with separate or combined exposure to AFB1 and MC-LR (EDIs ≥ mean). Meanwhile, after adjustment for confounding factors, means of AST and ALT and ORs of abnormal AST and ALT were successively elevated after exposure to HBV, HBV&AFB1 (or HBV&MC-LR), and HBV&AFB1&MC-LR, especially in the group with detectable HBV DNA (AST: OR = 11.38, 95%CI = 3.91-33.17; ALT: OR = 17.09, 95%CI = 5.36-54.53). Notably, ORs for abnormal AST and ALT in the HBV exposed group were not significantly different from those in HBV&AFB1 or in the HBV&MC-LR exposed group but were significantly higher in the HBV&AFB1&MC-LR exposed group (P = 0.029 and P = 0.037, respectively). Our study indicated that microcystin may have the potential to increase the risk of liver injury induced by combined exposure to HBV and aflatoxin. However, in consideration of the uncertainties in the detection of the toxins and evaluation of the EDIs, more epidemiological data are expected to determine the increasing toxic effects of microcystins.
[Mh] Termos MeSH primário: Vírus da Hepatite B
Hepatite B/epidemiologia
Microcistinas
[Mh] Termos MeSH secundário: Adulto
Aflatoxinas
Idoso
China/epidemiologia
Estudos Transversais
Feminino
Seres Humanos
Masculino
Meia-Idade
População Rural
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aflatoxins); 0 (Microcystins)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171215
[Lr] Data última revisão:
171215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1021/acs.est.6b05404


  9 / 2842 MEDLINE  
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[PMID]:27776235
[Au] Autor:Shi J; Deng H; Pan H; Xu Y; Zhang M
[Ad] Endereço:Key Laboratory of Yangtze River Water Environment, College of Environmental Science and Engineering, Tongji University, China.
[Ti] Título:Epigallocatechin-3-gallate attenuates microcystin-LR induced oxidative stress and inflammation in human umbilical vein endothelial cells.
[So] Source:Chemosphere;168:25-31, 2017 Feb.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Epigallocatechin-3-gallate (EGCG) has been shown to possess anti-inflammatory effects. Microcystin-LR (MC-LR) is a potent toxin and our past research suggested that it also mediated human umbilical vein endothelial cell (HUVEC) injury. The aim of this study was to investigate the effects of EGCG on MC-LR-induced oxidative stress and inflammatory responses in HUVECs. HUVECs were stimulated with MC-LR in the presence or absence of EGCG. MC-LR (40 µM) significantly increased cell death and decreased cell viability, migration, and tube formation, whereas EGCG (50 µM) inhibited these effects. Furthermore, the results indicated that EGCG inhibited the production of reactive oxygen species (ROS), tumor necrosis factor alpha (TNF-α), and interleukin-6 (IL-6) in MC-LR-stimulated HUVECs. Compared with MC-LR, EGCG significantly increased superoxide dismutase (SOD) and glutathione (GSH) levels and decreased malondialdehyde (MDA) levels. Moreover, the analysis indicated that EGCG suppressed MC-LR-induced NF-κB activation. In conclusion, the effects of EGCG were associated with inhibition of the NF-κB signaling pathway, which resulted in decreased ROS and TNF-α, thereby attenuating MC-LR-mediated oxidative and inflammatory responses.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Antioxidantes/farmacologia
Catequina/análogos & derivados
Inflamação/tratamento farmacológico
Microcistinas/efeitos dos fármacos
[Mh] Termos MeSH secundário: Catequina/farmacologia
Células Endoteliais da Veia Umbilical Humana
Seres Humanos
Inflamação/induzido quimicamente
Interleucina-6/metabolismo
Subunidade p50 de NF-kappa B/metabolismo
Espécies Reativas de Oxigênio/metabolismo
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antioxidants); 0 (IL6 protein, human); 0 (Interleukin-6); 0 (Microcystins); 0 (NF-kappa B p50 Subunit); 0 (NFKB1 protein, human); 0 (Reactive Oxygen Species); 0 (Tumor Necrosis Factor-alpha); 8R1V1STN48 (Catechin); BQM438CTEL (epigallocatechin gallate); EQ8332842Y (cyanoginosin LR)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171212
[Lr] Data última revisão:
171212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:28787682
[Au] Autor:Gaget V; Humpage AR; Huang Q; Monis P; Brookes JD
[Ad] Endereço:Water Research Centre, Ecology and Environmental Sciences, School of Biological Sciences, University of Adelaide, South Australia, 5005, Australia. Electronic address: virginie.gaget@adelaide.edu.au.
[Ti] Título:Benthic cyanobacteria: A source of cylindrospermopsin and microcystin in Australian drinking water reservoirs.
[So] Source:Water Res;124:454-464, 2017 Nov 01.
[Is] ISSN:1879-2448
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cyanobacteria represent a health hazard worldwide due to their production of a range of highly potent toxins in diverse aquatic environments. While planktonic species have been the subject of many investigations in terms of risk assessment, little is known about benthic forms and their impact on water quality or human and animal health. This study aimed to purify isolates from environmental benthic biofilms sampled from three different drinking water reservoirs and to assess their toxin production by using the following methods: Enzyme-Linked Immunosorbent Assay (ELISA), High-Performance Liquid Chromatography (HPLC), Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) and quantitative PCR (qPCR). Microscopic observation of the isolates allowed the identification of various filamentous cyanobacterial genera: Anabaena (benthic form), Calothrix and Nostoc from the Nostocales and Geitlerinema, Leptolyngbya, Limnothrix, Lyngbya, Oxynema, Phormidium and Pseudanabaena representing non-heterocystous filamentous cyanobacteria. The Phormidium ambiguum strain AWQC-PHO021 was found to produce 739 ng/mg of dry weight (d/w) of cylindrospermopsin and 107 ng/mg (d/w) of deoxy-cylindrospermopsin. The Nostoc linckia strain AWQC-NOS001 produced 400 ng/mg (d/w) of a microcystin analogue. This is the first report of hepatotoxin production by benthic cyanobacteria in temperate Australian drinking water reservoirs. These findings indicate that water quality monitoring programs need to consider benthic cyanobacteria as a potential source of toxins.
[Mh] Termos MeSH primário: Toxinas Bacterianas/análise
Cianobactérias/química
Água Potável
Microcistinas/análise
Uracila/análogos & derivados
[Mh] Termos MeSH secundário: Animais
Austrália
Seres Humanos
Espectrometria de Massas em Tandem
Uracila/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Toxins); 0 (Drinking Water); 0 (Microcystins); 2JIZ556BA3 (cylindrospermopsin); 56HH86ZVCT (Uracil)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170809
[St] Status:MEDLINE



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