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[PMID]:28415016
[Au] Autor:Duelge KJ; Nishshanka U; De Alwis HG
[Ad] Endereço:Food and Drug Administration, Center for Veterinary Medicine, Office of Research, 8401 Muirkirk Road, Laurel, MD 20708, USA.
[Ti] Título:An LC-MS/MS method for the determination of antibiotic residues in distillers grains.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1053:81-86, 2017 May 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Antibiotics are used in ethanol production to discourage the growth of bacteria that would result in lower ethanol content and a lower quality product. A survey conducted by the FDA (FY 2010 Nationwide Survey of Distillers Grains for Antibiotic Residues, 2009 [1]) revealed that the residues of these antibiotics can remain in the distillers grains (DG) by-product, which is used as an animal feed ingredient. The low levels of antibiotic residues in DG could be a public health concern, as they could lead to antimicrobial resistance. To enable the quantitative determination of these antibiotics (erythromycin, penicillin G, virginiamycin M1 and virginiamycin S1), we developed a sensitive LC-MS/MS method. The residues were extracted from distillers grains with a mixture of acetonitrile and buffer followed by acetonitrile. The combined extract was diluted with water and washed with hexane. An aliquot was cleaned up on an Oasis HLB solid phase extraction cartridge. Extracts were analyzed by LC-tandem mass spectrometry. The method was successfully validated using a variety of different matrices such as corn DG, corn & milo DG, and deoiled corn DG. Absolute recoveries of the analytes ranged from 53 to 106%. Accuracy ranged from 90 to 101% based on calibration by matrix standards. The limits of quantitation and relative standard deviation were all satisfactory to support future surveillance studies.
[Mh] Termos MeSH primário: Ração Animal/análise
Antibacterianos/análise
Eritromicina/análise
Penicilina G/análise
Estreptogramina A/análise
Estreptogramina Grupo B/análise
Espectrometria de Massas em Tandem/métodos
Virginiamicina/análise
[Mh] Termos MeSH secundário: Acetonitrilos/química
Animais
Cromatografia Líquida/métodos
Grãos Comestíveis/química
Hexanos/química
Limite de Detecção
Extração em Fase Sólida/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Acetonitriles); 0 (Anti-Bacterial Agents); 0 (Hexanes); 0 (Streptogramin Group B); 0 (virginiamycin factor S1); 11006-76-1 (Virginiamycin); 2DDG612ED8 (n-hexane); 63937KV33D (Erythromycin); 8W4UOL59AZ (Streptogramin A); Q42T66VG0C (Penicillin G); Z072SB282N (acetonitrile)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170418
[St] Status:MEDLINE


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[PMID]:26410170
[Au] Autor:Douarre PE; Sauvage E; Poyart C; Glaser P
[Ad] Endereço:Unité de Biologie des Bactéries pathogènes à Gram-positif, Institut Pasteur, 28 Rue du Dr Roux, 75724, Paris, France CNRS, UMR3525, Paris, France.
[Ti] Título:Host specificity in the diversity and transfer of lsa resistance genes in group B Streptococcus.
[So] Source:J Antimicrob Chemother;70(12):3205-13, 2015 Dec.
[Is] ISSN:1460-2091
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: In group B Streptococcus (GBS), cross-resistance to lincosamides, streptogramin A and pleuromutilins (LSAP) is mediated by the acquisition of lsa genes. Here, we characterized the diversity, mobility and ecology of lsa genes in this species. METHODS: lsa variants were systematically identified by BLAST searches in the genomes of 531 GBS strains from different hosts and geographical origins. The associated phenotypes were determined by a microdilution MIC method. Acquisition of resistance genes was deduced from comparative genomics and phylogeny. Their mobility was tested by conjugation experiments. RESULTS: lsa(E) and three variants of lsa(C) were identified in GBS strains. Two lsa(C) variants had not been previously reported. All four variants conferred LSAP phenotypes. lsa(E) was located in a multiresistance gene cluster of a single human strain. This gene was transferred by a high-frequency recombination-type mechanism between GBS strains. Two lsa(C) variants are carried in six unrelated human strains by two similar elements specifically integrated in the oriT site of four different classes of integrative and conjugative elements (ICEs). Strikingly, the acquisition of the resistance gene always occurred by the integration of the element into a resident ICE. The third lsa(C) variant was located at the same site in the core genome of 11 genetically distant bovine strains and was likely propagated by horizontal transfer of the corresponding chromosomal region. CONCLUSIONS: lsa genes in GBS show distinct host specificities and modes of transfer. In general, their dissemination is mediated by recombination rather than by the transfer of conjugative elements.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Farmacorresistência Bacteriana
Transferência Genética Horizontal
Genes Bacterianos
Especificidade de Hospedeiro
Streptococcus agalactiae/efeitos dos fármacos
Streptococcus agalactiae/genética
[Mh] Termos MeSH secundário: Animais
Bovinos
Doenças dos Bovinos/microbiologia
Diterpenos/farmacologia
Variação Genética
Infecções por Bactérias Gram-Positivas/microbiologia
Infecções por Bactérias Gram-Positivas/veterinária
Lincosamidas/farmacologia
Testes de Sensibilidade Microbiana
Recombinação Genética
Análise de Sequência de DNA
Homologia de Sequência
Streptococcus agalactiae/isolamento & purificação
Estreptogramina A/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Diterpenes); 0 (Lincosamides); 125-65-5 (pleuromutilin); 8W4UOL59AZ (Streptogramin A)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151120
[Lr] Data última revisão:
151120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150928
[St] Status:MEDLINE
[do] DOI:10.1093/jac/dkv277


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[PMID]:25891423
[Au] Autor:Wendlandt S; Kadlec K; Feßler AT; Schwarz S
[Ad] Endereço:Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Neustadt-Mariensee, Germany.
[Ti] Título:Identification of ABC transporter genes conferring combined pleuromutilin-lincosamide-streptogramin A resistance in bovine methicillin-resistant Staphylococcus aureus and coagulase-negative staphylococci.
[So] Source:Vet Microbiol;177(3-4):353-8, 2015 Jun 12.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to investigate the genetic basis of combined pleuromutilin-lincosamide-streptogramin A resistance in 26 unrelated methicillin-resistant Staphylococcus aureus (MRSA) and coagulase-negative staphylococci (CoNS) from dairy cows suffering from mastitis. The 26 pleuromutilin-resistant staphylococcal isolates were screened for the presence of the genes vga(A), vga(B), vga(C), vga(E), vga(E) variant, sal(A), vmlR, cfr, lsa(A), lsa(B), lsa(C), and lsa(E) by PCR. None of the 26 isolates carried the genes vga(B), vga(C), vga(E), vga(E) variant, vmlR, cfr, lsa(A), lsa(B), or lsa(C). Two Staphylococcus haemolyticus and single Staphylococcus xylosus, Staphylococcus lentus, and Staphylococcus hominis were vga(A)-positive. Twelve S. aureus, two Staphylococcus warneri, as well as single S. lentus and S. xylosus carried the lsa(E) gene. Moreover, single S. aureus, S. haemolyticus, S. xylosus, and Staphylococcus epidermidis were positive for both genes, vga(A) and lsa(E). The sal(A) gene was found in a single Staphylococcus sciuri. All ABC transporter genes were located in the chromosomal DNA, except for a plasmid-borne vga(A) gene in the S. epidermidis isolate. The genetic environment of the lsa(E)-positive isolates was analyzed using previously described PCR assays. Except for the S. warneri and S. xylosus, all lsa(E)-positive isolates harbored a part of the previously described enterococcal multiresistance gene cluster. This is the first report of the novel lsa(E) gene in the aforementioned bovine CoNS species. This is also the first identification of the sal(A) gene in a S. sciuri from a case of bovine mastitis. Moreover, the sal(A) gene was shown to also confer pleuromutilin resistance.
[Mh] Termos MeSH primário: Transportadores de Cassetes de Ligação de ATP/genética
Antibacterianos/farmacologia
Mastite Bovina/microbiologia
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Staphylococcus/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Bovinos
Coagulase/genética
Diterpenos/farmacologia
Farmacorresistência Bacteriana Múltipla/genética
Feminino
Lincosamidas/farmacologia
Mastite Bovina/tratamento farmacológico
Meticilina/farmacologia
Staphylococcus aureus Resistente à Meticilina/genética
Testes de Sensibilidade Microbiana
Infecções Estafilocócicas/microbiologia
Infecções Estafilocócicas/veterinária
Staphylococcus/enzimologia
Staphylococcus epidermidis/efeitos dos fármacos
Staphylococcus epidermidis/genética
Staphylococcus haemolyticus/efeitos dos fármacos
Staphylococcus haemolyticus/genética
Staphylococcus hominis/efeitos dos fármacos
Staphylococcus hominis/genética
Estreptogramina A/farmacologia
Estreptograminas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Coagulase); 0 (Diterpenes); 0 (Lincosamides); 0 (Streptogramins); 125-65-5 (pleuromutilin); 8W4UOL59AZ (Streptogramin A); Q91FH1328A (Methicillin)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150505
[Lr] Data última revisão:
150505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150421
[St] Status:MEDLINE


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[PMID]:25759293
[Au] Autor:Zhang A; Xu C; Wang H; Lei C; Liu B; Guan Z; Yang C; Yang Y; Peng L
[Ad] Endereço:Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life science, Sichuan University, Chengdu, Sichuan 610064, PR China; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Chengdu, Sichuan 610064, PR China.
[Ti] Título:Presence and new genetic environment of pleuromutilin-lincosamide-streptogramin A resistance gene lsa(E) in Erysipelothrix rhusiopathiae of swine origin.
[So] Source:Vet Microbiol;177(1-2):162-7, 2015 May 15.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Erysipelothrix rhusiopathiae is a Gram-positive bacillus that causes erysipelas in swine. In recent years, erysipelas infection among swine in China has been increasing. A combined resistance phenotype to pleuromutilins, lincosamides, and streptogramin A (PLSA phenotype) was found in some E. rhusiopathiae isolates. The aim of this study was to identify the resistance genes responsible for the PLSA phenotype in E. rhusiopathiae strains and to map the genetic environment of the identified resistance gene. A total of 46 E. rhusiopathiae isolates from 31 pig farms in China were studied. Minimum inhibitory concentrations (MICs) of 11 antimicrobial agents were determined by broth microdilution method. Seven were highly resistant to tiamulin (MICs 32 µg/ml) and clindamycin (MICs 64 µg/ml). Resistance genes responsible for the PLSA phenotype were screened by PCR. The lsa(E), spw, lnu(B), aadE and aphA3 genes were detected in strains had the PLSA phenotype, whereas none was detected in susceptible strains. The genetic environment of lsa(E) gene was determined by whole-genome sequencing and overlapping PCR assays. A novel multiresistance gene cluster, orf1-aadE-apt-spw-lsa(E)-lnu(B)-rec-orf2-orf1-aadE-sat4-aphA3, was found. Horizontal gene transfer experiments and whole-genome sequencing suggested that the lsa(E)-carrying multiresistance gene cluster was located in the chromosome. This is the first molecular characterization of PLSA resistance in E. rhusiopathiae. The lsa(E), spw and lnu(B) genes were found in E. rhusiopathiae for the first time. A novel lsa(E)-carrying multiresistance gene cluster was found. The location of lsa(E) in different gene cluster facilitates its persistence and dissemination.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Farmacorresistência Bacteriana Múltipla/genética
Erysipelothrix/efeitos dos fármacos
Erysipelothrix/genética
Genes MDR/genética
Suínos/microbiologia
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
China
Clindamicina/farmacologia
Diterpenos/farmacologia
Lincosamidas/farmacologia
Testes de Sensibilidade Microbiana
Dados de Sequência Molecular
Família Multigênica/genética
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Estreptogramina A/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Diterpenes); 0 (Lincosamides); 125-65-5 (pleuromutilin); 3U02EL437C (Clindamycin); 8W4UOL59AZ (Streptogramin A); E38WZ4U54R (tiamulin)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150406
[Lr] Data última revisão:
150406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150312
[St] Status:MEDLINE


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[PMID]:25708513
[Au] Autor:Li L; Zhao Y; Ruan L; Yang S; Ge M; Jiang W; Lu Y
[Ad] Endereço:Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, PR China.
[Ti] Título:A stepwise increase in pristinamycin II biosynthesis by Streptomyces pristinaespiralis through combinatorial metabolic engineering.
[So] Source:Metab Eng;29:12-25, 2015 May.
[Is] ISSN:1096-7184
[Cp] País de publicação:Belgium
[La] Idioma:eng
[Ab] Resumo:Pristinamycin, which is a streptogramin antibiotic produced by Streptomyces pristinaespiralis, contains two chemically unrelated compounds, pristinamycin I (PI) and pristinamycin II (PII). Semi-synthetic derivatives of PI and PII have been approved for use in human medicine to treat a broad range of drug-resistant pathogens. In this study, we design and implement a combinatorial metabolic engineering strategy for improving PII production. First, an extra copy of the PII biosynthetic gene cluster, which was assembled using a modified Gibson assembly method for cloning large DNA fragments with high GC contents, was introduced into a high-producing strain S. pristinaespiralis HCCB10218. This duplication of the PII biosynthetic gene cluster resulted in a maximum increase in PII titer by 45%. Second, all seven cluster-situated regulatory genes (from papR1 to papR6 and spbR) were systematically manipulated. Higher PII titers were achieved by deleting either one of the two repressor genes papR3 or papR5 in combination with overexpression of both activator genes papR4 and papR6, and the resulting strains ∆papR3+R4R6 and ∆papR5+R4R6 showed maximum increases in PII production by 99% and 75%, respectively. A combination of the above two different approaches was employed. Integration of the assembled PII gene cluster (BAC-F1F15) into ∆papR5+R4R6 led to the highest PII titer improvement, which was approximately 1.5-fold higher than the parental strain. By adding the macroreticular resin, which can separate pristinamycin in situ and thereby lessen end-product feedback inhibition and toxic effects, PII titers of the final engineered strain ∆papR5+R4R6/BAC-F1F15 reached 1.13 and 1.16g/L in the Erlenmeyer flask and 5-L bioreactor, respectively, with 5.13- and 5.26-fold improvements over the parental strain. Taken together, this combinatorial strategy is an efficient method to optimize PII biosynthesis of S. pristinaespiralis and may be extended to other industrially used streptomycetes for strain improvement.
[Mh] Termos MeSH primário: Proteínas de Bactérias
Regulação Bacteriana da Expressão Gênica
Genes Bacterianos
Estreptogramina A/biossíntese
Streptomyces
[Mh] Termos MeSH secundário: Proteínas de Bactérias/biossíntese
Proteínas de Bactérias/genética
Seres Humanos
Engenharia Metabólica
Streptomyces/genética
Streptomyces/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 8W4UOL59AZ (Streptogramin A)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150513
[Lr] Data última revisão:
150513
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150225
[St] Status:MEDLINE


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[PMID]:25404695
[Au] Autor:Dun J; Zhao Y; Zheng G; Zhu H; Ruan L; Wang W; Ge M; Jiang W; Lu Y
[Ad] Endereço:Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, People's Republic of China.
[Ti] Título:PapR6, a putative atypical response regulator, functions as a pathway-specific activator of pristinamycin II biosynthesis in Streptomyces pristinaespiralis.
[So] Source:J Bacteriol;197(3):441-50, 2015 Feb.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:There are up to seven regulatory genes in the pristinamycin biosynthetic gene cluster of Streptomyces pristinaespiralis, which infers a complicated regulation mechanism for pristinamycin production. In this study, we revealed that PapR6, a putative atypical response regulator, acts as a pathway-specific activator of pristinamycin II (PII) biosynthesis. Deletion of the papR6 gene resulted in significantly reduced PII production, and its overexpression led to increased PII formation, compared to that of the parental strain HCCB 10218. However, either papR6 deletion or overexpression had very little effect on pristinamycin I (PI) biosynthesis. Electrophoretic mobility shift assays (EMSAs) demonstrated that PapR6 bound specifically to the upstream region of snaF, the first gene of the snaFE1E2GHIJK operon, which is likely responsible for providing the precursor isobutyryl-coenzyme A (isobutyryl-CoA) and the intermediate C11 αß-unsaturated thioester for PII biosynthesis. A signature PapR6-binding motif comprising two 4-nucleotide (nt) inverted repeat sequences (5'-GAGG-4 nt-CCTC-3') was identified. Transcriptional analysis showed that inactivation of the papR6 gene led to markedly decreased expression of snaFE1E2GHIJK. Furthermore, we found that a mutant (snaFmu) with base substitutions in the identified PapR6-binding sequence in the genome exhibited the same phenotype as that of the ΔpapR6 strain. Therefore, it may be concluded that pathway-specific regulation of PapR6 in PII biosynthesis is possibly exerted via controlling the provision of isobutyryl-CoA as well as the intermediate C11 αß-unsaturated thioester.
[Mh] Termos MeSH primário: Regulação Bacteriana da Expressão Gênica
Estreptogramina A/biossíntese
Streptomyces/genética
Streptomyces/metabolismo
Fatores de Transcrição/metabolismo
[Mh] Termos MeSH secundário: Sítios de Ligação
Análise Mutacional de DNA
DNA Bacteriano/metabolismo
Ensaio de Desvio de Mobilidade Eletroforética
Deleção de Genes
Expressão Gênica
Perfilação da Expressão Gênica
Família Multigênica
Proteínas Mutantes/genética
Proteínas Mutantes/metabolismo
Óperon
Regiões Promotoras Genéticas
Ligação Proteica
Fatores de Transcrição/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Mutant Proteins); 0 (Transcription Factors); 8W4UOL59AZ (Streptogramin A)
[Em] Mês de entrada:1503
[Cu] Atualização por classe:151028
[Lr] Data última revisão:
151028
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141119
[St] Status:MEDLINE
[do] DOI:10.1128/JB.02312-14


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[PMID]:25223995
[Au] Autor:Stogios PJ; Kuhn ML; Evdokimova E; Courvalin P; Anderson WF; Savchenko A
[Ad] Endereço:Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada Center for Structural Genomics of Infectious Diseases (CSGID).
[Ti] Título:Potential for reduction of streptogramin A resistance revealed by structural analysis of acetyltransferase VatA.
[So] Source:Antimicrob Agents Chemother;58(12):7083-92, 2014 Dec.
[Is] ISSN:1098-6596
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Combinations of group A and B streptogramins (i.e., dalfopristin and quinupristin) are "last-resort" antibiotics for the treatment of infections caused by Gram-positive pathogens, including methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecium. Resistance to streptogramins has arisen via multiple mechanisms, including the deactivation of the group A component by the large family of virginiamycin O-acetyltransferase (Vat) enzymes. Despite the structural elucidation performed for the VatD acetyltransferase, which provided a general molecular framework for activity, a detailed characterization of the essential catalytic and antibiotic substrate-binding determinants in Vat enzymes is still lacking. We have determined the crystal structure of S. aureus VatA in apo, virginiamycin M1- and acetyl-coenzyme A (CoA)-bound forms and provide an extensive mutagenesis and functional analysis of the structural determinants required for catalysis and streptogramin A recognition. Based on an updated genomic survey across the Vat enzyme family, we identified key conserved residues critical for VatA activity that are not part of the O-acetylation catalytic apparatus. Exploiting such constraints of the Vat active site may lead to the development of streptogramin A compounds that evade inactivation by Vat enzymes while retaining binding to their ribosomal target.
[Mh] Termos MeSH primário: Acetiltransferases/química
Antibacterianos/química
Proteínas de Bactérias/química
Estreptogramina A/química
[Mh] Termos MeSH secundário: Acetilcoenzima A/química
Acetiltransferases/antagonistas & inibidores
Acetiltransferases/genética
Sequência de Aminoácidos
Proteínas de Bactérias/antagonistas & inibidores
Proteínas de Bactérias/genética
Domínio Catalítico
Cristalografia por Raios X
Farmacorresistência Bacteriana/genética
Expressão Gênica
Bactérias Gram-Negativas/química
Bactérias Gram-Negativas/enzimologia
Bactérias Gram-Positivas/química
Bactérias Gram-Positivas/enzimologia
Modelos Moleculares
Dados de Sequência Molecular
Mutagênese Sítio-Dirigida
Ligação Proteica
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Recombinant Proteins); 72-89-9 (Acetyl Coenzyme A); 8W4UOL59AZ (Streptogramin A); EC 2.3.1.- (Acetyltransferases)
[Em] Mês de entrada:1507
[Cu] Atualização por classe:161203
[Lr] Data última revisão:
161203
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140917
[St] Status:MEDLINE
[do] DOI:10.1128/AAC.03743-14


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[PMID]:24957822
[Au] Autor:Noeske J; Huang J; Olivier NB; Giacobbe RA; Zambrowski M; Cate JH
[Ad] Endereço:Department of Molecular Cell Biology, California Institute of Quantitative Biosciences, University of California, Berkeley, California, USA.
[Ti] Título:Synergy of streptogramin antibiotics occurs independently of their effects on translation.
[So] Source:Antimicrob Agents Chemother;58(9):5269-79, 2014 Sep.
[Is] ISSN:1098-6596
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Streptogramin antibiotics are divided into types A and B, which in combination can act synergistically. We compared the molecular interactions of the streptogramin combinations Synercid (type A, dalfopristin; type B, quinupristin) and NXL 103 (type A, flopristin; type B, linopristin) with the Escherichia coli 70S ribosome by X-ray crystallography. We further analyzed the activity of the streptogramin components individually and in combination. The streptogramin A and B components in Synercid and NXL 103 exhibit synergistic antimicrobial activity against certain pathogenic bacteria. However, in transcription-coupled translation assays, only combinations that include dalfopristin, the streptogramin A component of Synercid, show synergy. Notably, the diethylaminoethylsulfonyl group in dalfopristin reduces its activity but is the basis for synergy in transcription-coupled translation assays before its rapid hydrolysis from the depsipeptide core. Replacement of the diethylaminoethylsulfonyl group in dalfopristin by a nonhydrolyzable group may therefore be beneficial for synergy. The absence of general streptogramin synergy in transcription-coupled translation assays suggests that the synergistic antimicrobial activity of streptogramins can occur independently of the effects of streptogramin on translation.
[Mh] Termos MeSH primário: Antibacterianos/uso terapêutico
Biossíntese de Proteínas/efeitos dos fármacos
Estreptograminas/uso terapêutico
[Mh] Termos MeSH secundário: Antibacterianos/administração & dosagem
Antibacterianos/farmacologia
Cristalografia por Raios X
Combinação de Medicamentos
Sinergismo Farmacológico
Enterococcus faecalis/efeitos dos fármacos
Escherichia coli/efeitos dos fármacos
Haemophilus influenzae/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Ribossomos/efeitos dos fármacos
Ribossomos/ultraestrutura
Staphylococcus aureus/efeitos dos fármacos
Streptococcus pneumoniae/efeitos dos fármacos
Estreptogramina A/administração & dosagem
Estreptogramina A/farmacologia
Estreptogramina A/uso terapêutico
Estreptogramina B/administração & dosagem
Estreptogramina B/farmacologia
Estreptogramina B/uso terapêutico
Estreptograminas/administração & dosagem
Estreptograminas/química
Estreptograminas/farmacologia
Virginiamicina/administração & dosagem
Virginiamicina/farmacologia
Virginiamicina/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Drug Combinations); 0 (Streptogramins); 0 (flopristin, linopristin drug combination); 11006-76-1 (Virginiamycin); 126602-89-9 (quinupristin-dalfopristin); 3131-03-1 (Streptogramin B); 8W4UOL59AZ (Streptogramin A)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140625
[St] Status:MEDLINE
[do] DOI:10.1128/AAC.03389-14


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[PMID]:24687494
[Au] Autor:Hot C; Berthet N; Chesneau O
[Ad] Endereço:Institut Pasteur, Unité des Membranes Bactériennes, Paris, France.
[Ti] Título:Characterization of sal(A), a novel gene responsible for lincosamide and streptogramin A resistance in Staphylococcus sciuri.
[So] Source:Antimicrob Agents Chemother;58(6):3335-41, 2014 Jun.
[Is] ISSN:1098-6596
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Natural resistance to lincosamides and streptogramins A (LSA), which is a species characteristic of Bacillus subtilis and Enterococcus faecalis, has never been documented in the Staphylococcus genus. We investigate here the molecular basis of the LSA phenotype exhibited by seven reference strains of Staphylococcus sciuri, including the type strains of the three described subspecies. By whole-genome sequencing of strain ATCC 29059, we identified a candidate gene that encodes an ATP-binding cassette protein similar to the Lsa and VmlR resistance determinants. Isolation and reverse transcription-quantitative PCR (qRT-PCR) expression studies confirmed that Sal(A) can confer a moderate resistance to lincosamides (8 times the MIC of lincomycin) and a high-level resistance to streptogramins A (64 times the MIC of pristinamycin II). The chromosomal location of sal(A) between two housekeeping genes of the staphylococcal core genome supports the gene's ancient origins and thus innate resistance to these antimicrobials within S. sciuri subspecies.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Proteínas de Bactérias/genética
Farmacorresistência Bacteriana Múltipla
Lincosamidas/farmacologia
Infecções Estafilocócicas/microbiologia
Staphylococcus/genética
Estreptogramina A/farmacologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas de Bactérias/metabolismo
Mapeamento Cromossômico
DNA Bacteriano/química
DNA Bacteriano/genética
Testes de Sensibilidade Microbiana
Dados de Sequência Molecular
Fenótipo
Filogenia
Alinhamento de Sequência
Análise de Sequência de DNA
Staphylococcus/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (Lincosamides); 8W4UOL59AZ (Streptogramin A)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170424
[Lr] Data última revisão:
170424
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140402
[St] Status:MEDLINE
[do] DOI:10.1128/AAC.02797-13


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[PMID]:24379302
[Au] Autor:Li XS; Dong WC; Wang XM; Hu GZ; Wang YB; Cai BY; Wu CM; Wang Y; Du XD
[Ad] Endereço:College of Animal Husbandry and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
[Ti] Título:Presence and genetic environment of pleuromutilin-lincosamide-streptogramin A resistance gene lsa(E) in enterococci of human and swine origin.
[So] Source:J Antimicrob Chemother;69(5):1424-6, 2014 May.
[Is] ISSN:1460-2091
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Transportadores de Cassetes de Ligação de ATP/genética
Farmacorresistência Bacteriana
Enterococcus/isolamento & purificação
Infecções por Bactérias Gram-Positivas/veterinária
Lincosamidas/farmacologia
Estreptogramina A/farmacologia
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
Diterpenos/farmacologia
Enterococcus/efeitos dos fármacos
Ordem dos Genes
Infecções por Bactérias Gram-Positivas/microbiologia
Dados de Sequência Molecular
Análise de Sequência de DNA
Suínos
Doenças dos Suínos/microbiologia
[Pt] Tipo de publicação:LETTER; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Diterpenes); 0 (Lincosamides); 125-65-5 (pleuromutilin); 8W4UOL59AZ (Streptogramin A)
[Em] Mês de entrada:1411
[Cu] Atualização por classe:140408
[Lr] Data última revisão:
140408
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140101
[St] Status:MEDLINE
[do] DOI:10.1093/jac/dkt502



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