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  1 / 19 MEDLINE  
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[PMID]:28415016
[Au] Autor:Duelge KJ; Nishshanka U; De Alwis HG
[Ad] Endereço:Food and Drug Administration, Center for Veterinary Medicine, Office of Research, 8401 Muirkirk Road, Laurel, MD 20708, USA.
[Ti] Título:An LC-MS/MS method for the determination of antibiotic residues in distillers grains.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1053:81-86, 2017 May 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Antibiotics are used in ethanol production to discourage the growth of bacteria that would result in lower ethanol content and a lower quality product. A survey conducted by the FDA (FY 2010 Nationwide Survey of Distillers Grains for Antibiotic Residues, 2009 [1]) revealed that the residues of these antibiotics can remain in the distillers grains (DG) by-product, which is used as an animal feed ingredient. The low levels of antibiotic residues in DG could be a public health concern, as they could lead to antimicrobial resistance. To enable the quantitative determination of these antibiotics (erythromycin, penicillin G, virginiamycin M1 and virginiamycin S1), we developed a sensitive LC-MS/MS method. The residues were extracted from distillers grains with a mixture of acetonitrile and buffer followed by acetonitrile. The combined extract was diluted with water and washed with hexane. An aliquot was cleaned up on an Oasis HLB solid phase extraction cartridge. Extracts were analyzed by LC-tandem mass spectrometry. The method was successfully validated using a variety of different matrices such as corn DG, corn & milo DG, and deoiled corn DG. Absolute recoveries of the analytes ranged from 53 to 106%. Accuracy ranged from 90 to 101% based on calibration by matrix standards. The limits of quantitation and relative standard deviation were all satisfactory to support future surveillance studies.
[Mh] Termos MeSH primário: Ração Animal/análise
Antibacterianos/análise
Eritromicina/análise
Penicilina G/análise
Estreptogramina A/análise
Estreptogramina Grupo B/análise
Espectrometria de Massas em Tandem/métodos
Virginiamicina/análise
[Mh] Termos MeSH secundário: Acetonitrilos/química
Animais
Cromatografia Líquida/métodos
Grãos Comestíveis/química
Hexanos/química
Limite de Detecção
Extração em Fase Sólida/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Acetonitriles); 0 (Anti-Bacterial Agents); 0 (Hexanes); 0 (Streptogramin Group B); 0 (virginiamycin factor S1); 11006-76-1 (Virginiamycin); 2DDG612ED8 (n-hexane); 63937KV33D (Erythromycin); 8W4UOL59AZ (Streptogramin A); Q42T66VG0C (Penicillin G); Z072SB282N (acetonitrile)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170418
[St] Status:MEDLINE


  2 / 19 MEDLINE  
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Texto completo SciELO Brasil
[PMID]:27008373
[Au] Autor:Juda M; Chudzik-Rzad B; Malm A
[Ad] Endereço:Department of Pharmaceutical Microbiology, Medical University of Lublin, Lublin, Poland.
[Ti] Título:The prevalence of genotypes that determine resistance to macrolides, lincosamides, and streptogramins B compared with spiramycin susceptibility among erythromycin-resistant Staphylococcus epidermidis.
[So] Source:Mem Inst Oswaldo Cruz;111(3):155-60, 2016 Mar.
[Is] ISSN:1678-8060
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Coagulase-negative staphylococci, particularly Staphylococcus epidermidis, can be regarded as potential reservoirs of resistance genes for pathogenic strains, e.g., Staphylococcus aureus. The aim of this study was to assess the prevalence of different resistance phenotypes to macrolide, lincosamide, and streptogramins B (MLSB) antibiotics among erythromycin-resistant S. epidermidis, together with the evaluation of genes promoting the following different types of MLSB resistance:ermA, ermB, ermC,msrA, mphC, and linA/A'. Susceptibility to spiramycin was also examined. Among 75 erythromycin-resistantS. epidermidis isolates, the most frequent phenotypes were macrolides and streptogramins B (MSB) and constitutive MLSB (cMLSB). Moreover, all strains with the cMLSB phenotype and the majority of inducible MLSB (iMLSB) isolates were resistant to spiramycin, whereas strains with the MSB phenotype were sensitive to this antibiotic. The D-shape zone of inhibition around the clindamycin disc near the spiramycin disc was found for some spiramycin-resistant strains with the iMLSB phenotype, suggesting an induction of resistance to clindamycin by this 16-membered macrolide. The most frequently isolated gene was ermC, irrespective of the MLSB resistance phenotype, whereas the most often noted gene combination wasermC, mphC, linA/A'. The results obtained showed that the genes responsible for different mechanisms of MLSB resistance in S. epidermidis generally coexist, often without the phenotypic expression of each of them.
[Mh] Termos MeSH primário: Farmacorresistência Bacteriana Múltipla/genética
Genótipo
Lincosamidas/farmacologia
Macrolídeos/farmacologia
Staphylococcus epidermidis/efeitos dos fármacos
Staphylococcus epidermidis/genética
Estreptogramina Grupo B/farmacologia
[Mh] Termos MeSH secundário: Clindamicina/farmacologia
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão
Eritromicina/farmacologia
Testes Genéticos/métodos
Seres Humanos
Lincomicina/farmacologia
Fenótipo
Reação em Cadeia da Polimerase
Prevalência
Espiramicina/farmacologia
Staphylococcus epidermidis/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lincosamides); 0 (Macrolides); 0 (Streptogramin Group B); 3U02EL437C (Clindamycin); 63937KV33D (Erythromycin); 8025-81-8 (Spiramycin); BOD072YW0F (Lincomycin)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160324
[St] Status:MEDLINE


  3 / 19 MEDLINE  
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[PMID]:25706726
[Au] Autor:Aydeniz Ozansoy F; Cevahir N; Kaleli I
[Ti] Título:[Investigation of macrolide, lincosamide and streptogramin B resistance in Staphylococcus aureus strains isolated from clinical samples by phenotypical and genotypical methods].
[Ti] Título:Klinik örneklerden izole edilen Staphylococcus aureus suslarinda makrolid, linkozamid ve streptogramin B direncinin fenotipik ve genotipik yöntemlerle arastirilmasi.
[So] Source:Mikrobiyol Bul;49(1):1-14, 2015 Jan.
[Is] ISSN:0374-9096
[Cp] País de publicação:Turkey
[La] Idioma:tur
[Ab] Resumo:Staphylococcus aureus is one of the most common cause of both community and healthcare-associated infections. As staphylococci have developed resistance to various antibiotics, initially to penicillins then to methicillin and glycopeptides and have the ability to cause epidemics, they continue to be a major problem from past to present. Methicillin resistance gave rise to the use of alternative antibiotics such as macrolides, however worldwide development of macrolide resistance limited the use of these antibiotics. Macrolide resistance occurs either through target site modification (MLS(B) phenotype, encoded by erm genes), efflux pumps (MS phenotype, encoded by msrA/B genes) or decreased cell wall permeability. The aim of this study was to investigate the MLS(B) resistance of clinical S.aureus strains with phenotypic and genotypic methods. A total of 404 S.aureus strains isolated from different clinical samples (50% wound, 15% tracheal aspirate and 35% other samples) of inpatients (93.3%) and outpatients (6.7%) were included in the study. Double disc synergy test (D-test) was used for the phenotypical research and PCR was used for the genotypical research of MLS(B) resistance of isolates. One hundred fifty eight (39.1%) of the S.aureus isolates were methicillin-resistant (MRSA), and 246 (60.9%) were methicillin-susceptible (MSSA). By the use of D-test, constitutive (cMLS(B)) and inducible (iMLS(B)) clindamycin resistance were detected in 19 and 111 isolates, respectively, while five isolates were MS phenotype and 268 isolates were S phenotype (susceptible to erythromycin and clindamycin). The resistance genes of 136 isolates with MLS(B) resistance phenotype were determined genotypically and among 111 isolates showing iMLS(B) phenotype ermA gene was found in 81.9% (83 MRSA, 8 MSSA), ermC gene in 10.8% (7 MRSA, 5 MSSA), msrA gene in 10.8% (11 MRSA, 1 MSSA), msrB gene in 1.8% (2 MRSA) and ermB gene in 0.9% (1 MRSA). Among 19 strains with cMLS(B) phenotype, ermA was found in 57.9% (10 MRSA, 1 MSSA), ermC in 36.8% (6 MRSA, 1 MSSA) and ermB in 15.8% (3 MRSA). Among five strains with MS phenotype, ermA was found in 80% (2 MRSA, 2 MSSA), msrA in 75% (3 MSSA), msrB in 50% (2 MSSA) and ermC in 25% (1 MSSA) of the isolates. ErmA and ermC genes were detected together in 14 isolates, ermA, ermC and msrA genes in one isolate, ermA and msrA genes in 11 isolates, ermA, msrA and msrB genes in three isolates and ermA and ermB genes in three isolates, respectively. In this study, two MRSA isolates with MS phenotype and negative D-test had only ermA gene and among two MSSA strains, erm genes were also determined in addition to msr genes. In our study RAPD-PCR method was used to investigate the clonal similarity, however no dominance of one or a number of clonal type was observed among the isolates in which the resistance genes were identified. In conclusion, the detection of MLS(B) resistance in S.aureus isolates is likely to influence the selection of antibiotics in the treatment of the infections caused by this bacteria.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Lincosamidas/farmacologia
Macrolídeos/farmacologia
Infecções Estafilocócicas/microbiologia
Staphylococcus aureus/efeitos dos fármacos
Estreptogramina Grupo B/farmacologia
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão
Genótipo
Seres Humanos
Pacientes Internados
Staphylococcus aureus Resistente à Meticilina/classificação
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Staphylococcus aureus Resistente à Meticilina/genética
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Pacientes Ambulatoriais
Fenótipo
Reação em Cadeia da Polimerase
Técnica de Amplificação ao Acaso de DNA Polimórfico
Staphylococcus aureus/classificação
Staphylococcus aureus/genética
Staphylococcus aureus/isolamento & purificação
Traqueia/microbiologia
Ferimentos e Lesões/microbiologia
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Lincosamides); 0 (Macrolides); 0 (Streptogramin Group B)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:150224
[Lr] Data última revisão:
150224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150224
[St] Status:MEDLINE


  4 / 19 MEDLINE  
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[PMID]:24308503
[Au] Autor:Heß S; Gallert C
[Ad] Endereço:Institute of Biology for Engineers and Biotechnology of Wastewater Treatment, Karlsruhe Institute of Technology, Karlsruhe, Germany.
[Ti] Título:Demonstration of staphylococci with inducible macrolide-lincosamide-streptogramin B (MLSB ) resistance in sewage and river water and of the capacity of anhydroerythromycin to induce MLSB.
[So] Source:FEMS Microbiol Ecol;88(1):48-59, 2014 Apr.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Staphylococci causing diseases in humans and animals are well described, whereas not very much is known about the staphylococci in natural ecosystems. Due to increased consumption of antibiotics, multiresistant species are released with excrements. Therefore, 1048 staphylococci from raw and treated sewage and from receiving water bodies were isolated, identified and tested for resistance against erythromycin, clindamycin, oxacillin and ciprofloxacin. More resistant staphylococci were present in raw sewage (33.8%) than in treated sewage (24.9%) or river water (10.9%). Of all isolates, 20.2% were resistant against the macrolide erythromycin which can induce cross-resistance against lincosamides and streptogramin B antibiotics (iMLSB ). Erythromycin is metabolized to anhydroerythromycin and excreted with urine into sewage. The question arises whether anhydroerythromycin can also induce resistance against MLSB antibiotics in staphylococci. This was investigated with antibiotic susceptibility tests (D-tests) and macrodilution assays. Staphylococci with iMLSB phenotype in river water were more numerous (27.8%) than in treated sewage (18.9%). The most common MLSB resistance gene was ermC. Traces of erythromycin and anhydroerythromycin (1 ng L(-1) ) induced already resistance against clindamycin after only 10 min exposure. This is reported for the first time and is relevant for risk assessment.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Farmacorresistência Bacteriana Múltipla
Lincosamidas/farmacologia
Macrolídeos/farmacologia
Esgotos/microbiologia
Staphylococcus/efeitos dos fármacos
Estreptogramina Grupo B/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/uso terapêutico
Água Potável/microbiologia
Lincosamidas/uso terapêutico
Macrolídeos/uso terapêutico
Rios/microbiologia
Staphylococcus/classificação
Staphylococcus/genética
Estreptogramina Grupo B/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Drinking Water); 0 (Lincosamides); 0 (Macrolides); 0 (Sewage); 0 (Streptogramin Group B)
[Em] Mês de entrada:1407
[Cu] Atualização por classe:140404
[Lr] Data última revisão:
140404
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131207
[St] Status:MEDLINE
[do] DOI:10.1111/1574-6941.12268


  5 / 19 MEDLINE  
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[PMID]:24206069
[Au] Autor:Thumu SC; Halami PM
[Ad] Endereço:Food Microbiology Department, CSIR-Central Food Technological Research Institute, Mysore, India.
[Ti] Título:Phenotypic expression, molecular characterization and transferability of erythromycin resistance genes in Enterococcus spp. isolated from naturally fermented food.
[So] Source:J Appl Microbiol;116(3):689-99, 2014 Mar.
[Is] ISSN:1365-2672
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: To evaluate phenotypic resistance to macrolides-lincosamides and streptogramin B (MLSB ) antibiotics and to determine their localization as well as transferability of erythromycin resistance genes in enterococcal isolates of naturally fermented food-Idli batter. METHODS AND RESULTS: Diverse MLSB phenotypes observed among the enterococcal spp. (n = 32) were analysed through double disc and triple disc test. Standard minimum inhibitory concentration tests along with induction studies displayed synergistic or cross-resistance among MLSB antibiotics. Plasmid profiling and Southern hybridization revealed that erm(B) and msr(C) genes were localized either on chromosome or on high molecular weight plasmids and showed co-localization of these genes with lnu(B), tet(L) and tet(W) in one of the isolate. In vitro conjugation experiments demonstrated plasmid-mediated transfer of erm(B) gene from three Enterococcus durans strains to Enterococcus faecalis JH2-2. CONCLUSIONS: The study illustrated diverse MLSB phenotypes, multiple resistance genes and transferable plasmids among enterococci isolated from naturally fermented foods. SIGNIFICANCE AND IMPACT OF THE STUDY: From a public health point of view, the study identified that naturally fermented foods could represent a source of antibiotic resistance enterococci that can spread through foods. These results also suggest that vigilance may be exercised with the use of combination or novel MLSB antibiotics in treating enterococcal infections.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Enterococcus/efeitos dos fármacos
Enterococcus/genética
Eritromicina/farmacologia
Microbiologia de Alimentos
[Mh] Termos MeSH secundário: Clindamicina/farmacologia
Conjugação Genética
Farmacorresistência Bacteriana/genética
Enterococcus/isolamento & purificação
Fermentação
Genes Bacterianos
Lincosamidas/farmacologia
Macrolídeos/farmacologia
Fenótipo
Plasmídeos/genética
Estreptogramina Grupo B/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Lincosamides); 0 (Macrolides); 0 (Streptogramin Group B); 3U02EL437C (Clindamycin); 63937KV33D (Erythromycin)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:150202
[Lr] Data última revisão:
150202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131112
[St] Status:MEDLINE
[do] DOI:10.1111/jam.12386


  6 / 19 MEDLINE  
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Bachion, Maria Márcia
Texto completo SciELO Brasil
[PMID]:23295875
[Au] Autor:Martins MA; Santos Sde L; Leão LS; Araújo NP; Bachion MM
[Ad] Endereço:Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal de Goiás, Goiânia, GO. marlenianapower@hotmail.com
[Ti] Título:Prevalence of resistance phenotypes in Staphylococcus aureus and coagulase-negative isolates of venous ulcers of primary healthcare patients.
[So] Source:Rev Soc Bras Med Trop;45(6):717-22, 2012 Dec.
[Is] ISSN:1678-9849
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: In venous ulcers, the presence of Staphylococcus aureus and coagulase-negative staphylococcus resistance phenotypes can aggravate and limit the choices for treatment. METHODS: Staphylococcus isolated from 69 patients (98 ulcers) between October of 2009 and October of 2010 were tested. The macrolide, lincosamide, streptogramin B (MLS B) group resistance phenotype detection was performed using the D-test. Isolates resistant to cefoxitin and/or oxacillin (disk-diffusion) were subjected to the confirmatory test to detect minimum inhibitory concentration (MIC), using oxacillin strips (E-test®). RESULTS: The prevalence of S. aureus was 83%, and 15% of coagulase-negative staphylococcus (CoNS). In addition were detected 28% of methicillin-resistant Staphylococcus aureus (MRSA) and 47% of methicillin-resistant coagulase-negative staphylococcus (MRCoNS). Among the S. aureus, 69.6% were resistant to erythromycin, 69.6% to clindamycin, 69.6% to gentamicin, and 100% to ciprofloxacin. Considering the MRSA, 74% were highly resistant to oxacillin, MIC ≥ 256µg/mL, and the MLS Bc constitutive resistance predominated in 65.2%. Among the 20 isolates sensitive to clindamycin, 12 presented an inducible MLS B phenotype. Of the MRCoNS, 71.4%were resistant to erythromycin, ciprofloxacin and gentamicin. Considering the isolates positive for ß-lactamases, the MIC breakpoint was between 0.5 and 2µg/mL. CONCLUSIONS: The results point to a high occurrence of multi-drug resistant bacteria in venous ulcers in primary healthcare patients, thus evidencing the need for preventive measures to avoid outbreaks caused by multi-drug resistant pathogens, and the importance of healthcare professionals being able to identifying colonized versus infected venous ulcers as an essential criteria to implementing systemic antibacterial therapy.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Lincosamidas/farmacologia
Macrolídeos/farmacologia
Staphylococcus aureus/efeitos dos fármacos
Estreptogramina Grupo B/farmacologia
Úlcera Varicosa/microbiologia
[Mh] Termos MeSH secundário: Coagulase/metabolismo
Estudos Transversais
Farmacorresistência Bacteriana Múltipla
Feminino
Seres Humanos
Masculino
Staphylococcus aureus Resistente à Meticilina
Testes de Sensibilidade Microbiana/métodos
Meia-Idade
Fenótipo
Prevalência
Atenção Primária à Saúde
Staphylococcus aureus/classificação
Staphylococcus aureus/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Coagulase); 0 (Lincosamides); 0 (Macrolides); 0 (Streptogramin Group B)
[Em] Mês de entrada:1307
[Cu] Atualização por classe:130108
[Lr] Data última revisão:
130108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130109
[St] Status:MEDLINE


  7 / 19 MEDLINE  
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[PMID]:22934408
[Au] Autor:Qiu Y; Yang F; Liu Z; Lin Y; Liu S
[Ad] Endereço:Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou 350001, China. yjqiuzju@163.com
[Ti] Título:[Determination of virginiamycin M1 and S1 residues in livestock and poultry products by liquid chromatography-tandem mass spectrometry].
[So] Source:Se Pu;30(5):463-7, 2012 May.
[Is] ISSN:1000-8713
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:A liquid chromatography-tandem mass spectrometry method was established for the determination of virginiamycin M1 and S1 residues in livestock and poultry products. The sample was extracted by methanol-acetonitrile solution (1:1, v/v). The supernatant was diluted with 0.01 mol/L ammonium dihydrogen phosphate solution, then purified and concentrated on an Oasis HLB cartridge. The separation of virginiamycin M1 and S1 was performed on a Luna C18 column with the mobile phases acetonitrile and 5 mmol/L ammonium acetate aqueous solution (containing 0.1% (v/v) formic acid) in a gradient elution mode. The identification and quantification of the drugs were carried out by positive electrospray ionization (ESI + ) in the multiple reaction monitoring (MRM) mode using external standard method. The calibration curves showed good linearity in the range of 0.15-10.0 microg/L with correlation coefficients (r2) above 0. 999. The limits of quantities (LOQs) were both 0.25 microg/kg. The average recoveries of the two drugs spiked at 0.25, 0.5 and 2.5 microg/kg levels in different matrices were between 71.2% and 98.4%, and the relative standard deviations (RSDs) were between 3.6% and 15.4%. The method is simple, rapid, sensitive and accurate. It is suitable for the confirmation and quantification of virginiamycin M1 and S1 residues in livestock and poultry products.
[Mh] Termos MeSH primário: Cromatografia Líquida/métodos
Produtos da Carne/análise
Estreptogramina A/análise
Estreptogramina Grupo B/análise
Espectrometria de Massas em Tandem/métodos
Virginiamicina/análise
[Mh] Termos MeSH secundário: Animais
Resíduos de Drogas/análise
Contaminação de Alimentos/análise
Gado
Aves Domésticas
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Streptogramin Group B); 0 (virginiamycin factor S1); 11006-76-1 (Virginiamycin); 8W4UOL59AZ (Streptogramin A)
[Em] Mês de entrada:1309
[Cu] Atualização por classe:161018
[Lr] Data última revisão:
161018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120901
[St] Status:MEDLINE


  8 / 19 MEDLINE  
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[PMID]:22639306
[Au] Autor:Sakar H; Mumcuoglu I; Aksu N; Karahan ZC; Kursun S; Kustimur S
[Ad] Endereço:Ankara Numune Training and Research Hospital, Department of Medical Microbiology, Ankara, Turkey.
[Ti] Título:[The rare genes related to resistance to macrolide-lincosamide and streptogramin B group antibiotics among coagulase-negative staphylococci].
[Ti] Título:Koagülaz-Negatif Stafilokoklarda Makrolid-Linkozamid-Streptogramin B Grubu Antibiyotiklere Karsi Nadir Direnç Genlerinin Arastirilmasi.
[So] Source:Mikrobiyol Bul;46(2):170-9, 2012 Apr.
[Is] ISSN:0374-9096
[Cp] País de publicação:Turkey
[La] Idioma:tur
[Ab] Resumo:Macrolide-lincosamide-streptogramin B (MLSB) group antibiotics are recommended as first choice in the treatment of staphylococcal infections. All of those drugs bind to the 50S subunit of bacterial ribosomes, thus cross-resistance is a major concern in this group of drugs. The mechanisms associated to resistance are (a) ribosomal methylation due to the methylases encoded by erm genes, (b) active drug efflux due to msrA, msrB, vga, vgb gene activity, (c) enzymatic inactivation of the drug due to the activity of linA, vat, vatB genes. While the most common resistance genes are ermA, ermB, ermC, msrA and msrB genes; linA, vga, vgb, vat and vatB genes have also been found in some studies. In this study it was aimed to investigate the presence of the rare MLSB resistance genes and their coexistence with erm and msr genes in 454 clinical isolates of coagulase-negative staphylococci (CNS). Of them 46.5% (n= 211) were S.hominis, 30.8% (n= 140) were S.epidermidis, 12.1% (n= 55) were S.haemolyticus, 3.5% (n= 16) were S.warnerii and 7% (n= 32) were the other coagulase-negative staphylococcal species. Resistance phenotypes were determined by using D-test method according to the recommendation of Clinical and Laboratory Standards Institute (CLSI). With the D-test 107 (23.6%) strains were determined as M phenotype (resistant to erythromycin and inducible clindamycin resistance was not detected), 92 (20.3%) were iMLSB phenotype (inducible clindamycin resistance was detected by the D-test) and 110 (24.2%) were cMLSB phenotype (constitutive erythromycin and clindamycin resistance was detected). linA, vga, vgb, vat, vatB, ermA, ermB, ermC, msrA, msrB genes were investigated by polymerase chain reaction in all strains showing iMLSB (n= 92) and cMLSB (n= 110) phenotypes and 46 randomly selected strains among 107 strains exhibiting the M phenotype. linA gene was found in 91 (20%) strains as single gene or in combination with erm or msr genes, and vga gene was found in 19 (4.2%) strains. linA gene was found in 52% of iMLSB phenotype, in 26% of cMLSB phenotype and 13% of M phenotype while vga gene was found in 5.4% of iMLSB phenotype, in 12% of cMLSB phenotype and in 0.9% of M phenotype. The most common resistance gene among iMLSB and cMLSB phenotypes was ermC (32.6% and 42.7%, respectively), followed by ermC + linA gene combination (31.5% and 14.5%, respectively). The most frequent gene combination was msrA and msrB in M phenotype (34.8%) and it was followed by a combination of msrA + msrB + linA genes (19.1%). None of the strains revealed presence of vgb, vat and vatB genes. There were no previous reports about the rarely detected resistance genes against MLSB antibiotics in our country. This was the first study which reported the frequency of linA, vga, vgb, vat and vatB genes in MLSB resistant CNS. In conclusion, since linA and vga genes were detected in high frequency in MLSB resistant CNS in this study, it was thought that the investigation of these genes should be included in the further related epidemiologic gene research.
[Mh] Termos MeSH primário: Farmacorresistência Bacteriana Múltipla/genética
Lincosamidas/farmacologia
Macrolídeos/farmacologia
Staphylococcus/efeitos dos fármacos
Staphylococcus/genética
Estreptogramina Grupo B/farmacologia
[Mh] Termos MeSH secundário: Coagulase
Seres Humanos
Infecções Estafilocócicas/tratamento farmacológico
Infecções Estafilocócicas/microbiologia
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coagulase); 0 (Lincosamides); 0 (Macrolides); 0 (Streptogramin Group B)
[Em] Mês de entrada:1302
[Cu] Atualização por classe:120528
[Lr] Data última revisão:
120528
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120529
[St] Status:MEDLINE


  9 / 19 MEDLINE  
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[PMID]:21816878
[Au] Autor:Ningsih F; Kitani S; Fukushima E; Nihira T
[Ad] Endereço:International Center for Biotechnology, Osaka University, 2-1, Yamadaoka, Suita, Osaka 565-0871, Japan.
[Ti] Título:VisG is essential for biosynthesis of virginiamycin S, a streptogramin type B antibiotic, as a provider of the nonproteinogenic amino acid phenylglycine.
[So] Source:Microbiology;157(Pt 11):3213-20, 2011 Nov.
[Is] ISSN:1465-2080
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A streptogramin type B antibiotic, virginiamycin S (VS), is produced by Streptomyces virginiae, together with a streptogramin type A antibiotic, virginiamycin M1 (VM), as its synergistic counterpart. VS is a cyclic hexadepsipeptide containing a nonproteinogenic amino acid, Lphenylglycine (L-pheGly), in its core structure. We have identified, in the left-hand extremity of the virginiamycin supercluster, two genes that direct VS biosynthesis with L-pheGly incorporation. Transcriptional analysis revealed that visF, encoding a nonribosomal peptide synthetase, and visG, encoding a protein with homology to a hydroxyphenylacetyl-CoA dioxygenase, are under the transcriptional regulation of virginiae butanolide (VB), a small diffusing signalling molecule that governs virginiamycin production. Gene deletion of visG resulted in complete loss of VS production without any changes in VM production, suggesting that visG is required for VS biosynthesis. The abolished VS production in the visG disruptant was fully recovered either by the external addition of pheGly or by gene complementation, which indicates that VisG is involved in VS biosynthesis as the provider of an L-pheGly molecule. A feeding experiment with L-pheGly analogues suggested that VisF, which is responsible for the last condensation step, has high substrate specificity toward L-pheGly.
[Mh] Termos MeSH primário: Antibacterianos/biossíntese
Proteínas de Bactérias/metabolismo
Glicina/análogos & derivados
Estreptogramina Grupo B/biossíntese
Streptomyces/metabolismo
Virginiamicina/biossíntese
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas de Bactérias/genética
Clonagem Molecular
Deleção de Genes
Regulação Bacteriana da Expressão Gênica
Teste de Complementação Genética
Dados de Sequência Molecular
Família Multigênica
RNA Bacteriano/genética
Streptomyces/genética
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (RNA, Bacterial); 0 (Streptogramin Group B); 0 (virginiamycin factor S1); 11006-76-1 (Virginiamycin); TE7660XO1C (Glycine)
[Em] Mês de entrada:1203
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110806
[St] Status:MEDLINE
[do] DOI:10.1099/mic.0.050203-0


  10 / 19 MEDLINE  
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[PMID]:21131144
[Au] Autor:Bilk S; Nordhoff M; Schulze C; Wieler LH; Kutzer P
[Ad] Endereço:Landeslabor Berlin-Brandenburg, Gerhard-Neumann-Str. 2, 15236 Frankfurt (Oder), Germany.
[Ti] Título:Antimicrobial susceptibilities and occurrence of resistance genes in bovine Helcococcus ovis isolates.
[So] Source:Vet Microbiol;149(3-4):488-91, 2011 May 05.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The aim of the study was to investigate antimicrobial susceptibilities of bovine Helcococcus ovis isolates and to detect genes encoding for H. ovis erythromycin and tetracycline resistance. Twenty-nine isolates were included and the minimal inhibitory concentrations (MICs) of seven antimicrobials were determined using test conditions as given in an approved CLSI guideline for the pyridoxal-dependent Abiotrophia spp. and Granulicatella spp. Furthermore, the macrolide resistance phenotype was examined by the erythromycin-clindamycin double-disk test (D-zone test). Erythromycin MICs of ≥ 8 µg/ml were found in three (10%) isolates which also presented the macrolide, lincosamide, and streptogramin B (MLS(B)) resistance phenotype, either constitutive or inducible. The erm(B) gene was detected in one of these isolates. Increased tetracycline MICs (≥ 8 µg/ml) were obtained for 24 (83%) isolates, mostly associated with the tet(M) gene alone (n=21) or both the tet(L) and tet(M) genes (n=2). The MICs determined for penicillin, ampicillin, amoxicillin-clavulanic acid, and cephalothin did not indicate resistance to these antimicrobials. The study suggests that resistance to MLS(B) antimicrobials and tetracycline is frequent in H. ovis. Moreover, this is the first report about occurrence of the resistance genes erm(B), tet(L), and tet(M) in the Helcococcus genus.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Farmacorresistência Bacteriana/genética
Bacilos Gram-Positivos Formadores de Endosporo/efeitos dos fármacos
Bacilos Gram-Positivos Formadores de Endosporo/genética
[Mh] Termos MeSH secundário: Animais
Bovinos/microbiologia
Clindamicina/farmacologia
Eritromicina/farmacologia
Lincosamidas/farmacologia
Macrolídeos/farmacologia
Testes de Sensibilidade Microbiana
Fenótipo
Estreptogramina Grupo B/farmacologia
Tetraciclina/farmacologia
Resistência a Tetraciclina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Lincosamides); 0 (Macrolides); 0 (Streptogramin Group B); 3U02EL437C (Clindamycin); 63937KV33D (Erythromycin); F8VB5M810T (Tetracycline)
[Em] Mês de entrada:1205
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:101207
[St] Status:MEDLINE
[do] DOI:10.1016/j.vetmic.2010.11.012



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