Base de dados : MEDLINE Pesquisa : D04.345.566.802.750.500 [Categoria DeCS] Referências encontradas : 105 [refinar] Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 11

ir para página

1 / 105

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 28270110 [Au] Autor: Beukers AG; Zaheer R; Goji N; Amoako KK; Chaves AV; Ward MP; McAllister TA [Ad] Endereço: Faculty of Veterinary Science, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW, Australia. [Ti] Título: Comparative genomics of Enterococcus spp. isolated from bovine feces. [So] Source: BMC Microbiol;17(1):52, 2017 Mar 08. [Is] ISSN: 1471-2180 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Enterococcus is ubiquitous in nature and is a commensal of both the bovine and human gastrointestinal (GI) tract. It is also associated with clinical infections in humans. Subtherapeutic administration of antibiotics to cattle selects for antibiotic resistant enterococci in the bovine GI tract. Antibiotic resistance genes (ARGs) may be present in enterococci following antibiotic use in cattle. If located on mobile genetic elements (MGEs) their dissemination between Enterococcus species and to pathogenic bacteria may be promoted, reducing the efficacy of antibiotics. RESULTS: We present a comparative genomic analysis of twenty-one Enterococcus spp. isolated from bovine feces including Enterococcus hirae (n = 10), Enterococcus faecium (n = 3), Enterococcus villorum (n = 2), Enterococcus casseliflavus (n = 2), Enterococcus faecalis (n = 1), Enterococcus durans (n = 1), Enterococcus gallinarum (n = 1) and Enterococcus thailandicus (n = 1). The analysis revealed E. faecium and E. faecalis from bovine feces share features with human clinical isolates, including virulence factors. The Tn917 transposon conferring macrolide-lincosamide-streptogramin B resistance was identified in both E. faecium and E. hirae, suggesting dissemination of ARGs on MGEs may occur in the bovine GI tract. An E. faecium isolate was also identified with two integrative conjugative elements (ICEs) belonging to the Tn916 family of ICE, Tn916 and Tn5801, both conferring tetracycline resistance. CONCLUSIONS: This study confirms the presence of enterococci in the bovine GI tract possessing ARGs on MGEs, but the predominant species in cattle, E. hirae is not commonly associated with infections in humans. Analysis using additional complete genomes of E. faecium from the NCBI database demonstrated differential clustering of commensal and clinical isolates, suggesting that these strains may be specifically adapted to their respective environments. [Mh] Termos MeSH primário: Bovinos/microbiologia Enterococcus/classificação Enterococcus/genética Enterococcus/isolamento & purificação Fezes/microbiologia Genoma Bacteriano/genética Genômica [Mh] Termos MeSH secundário: Animais Antibacterianos/farmacologia Bacteriófagos Sistemas CRISPR-Cas Doenças dos Bovinos/microbiologia Análise por Conglomerados Elementos de DNA Transponíveis/genética DNA Bacteriano/isolamento & purificação Farmacorresistência Bacteriana Múltipla/genética Enterococcus/efeitos dos fármacos Enterococcus faecalis/efeitos dos fármacos Enterococcus faecalis/genética Enterococcus faecalis/isolamento & purificação Enterococcus faecalis/patogenicidade Enterococcus faecium/efeitos dos fármacos Enterococcus faecium/genética Enterococcus faecium/isolamento & purificação Enterococcus faecium/patogenicidade Enterococcus hirae/efeitos dos fármacos Enterococcus hirae/genética Enterococcus hirae/isolamento & purificação Enterococcus hirae/patogenicidade Microbioma Gastrointestinal Seres Humanos Sequências Repetitivas Dispersas/genética Lincosamidas/farmacologia Macrolídeos/farmacologia Testes de Sensibilidade Microbiana Tipagem de Sequências Multilocus/métodos Filogenia Polimorfismo de Nucleotídeo Único Estreptogramina B/farmacologia Resistência a Tetraciclina/genética Fatores de Virulência/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (DNA Transposable Elements); 0 (DNA, Bacterial); 0 (Lincosamides); 0 (Macrolides); 0 (Virulence Factors); 3131-03-1 (Streptogramin B) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170925 [Lr] Data última revisão: 170925 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170309 [St] Status: MEDLINE [do] DOI: 10.1186/s12866-017-0962-1

2 / 105

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 28231812 [Au] Autor: Foster-Nyarko E; Kwambana B; Ceesay F; Jawneh K; Darboe S; Mulwa SN; Ceesay B; Secka OO; Adetifa I; Antonio M [Ad] Endereço: Vaccines and Immunity Theme, Medical Research Council Unit The Gambia, Banjul, The Gambia. [Ti] Título: Incidence of macrolide-lincosamide-streptogramin B resistance amongst beta-haemolytic streptococci in The Gambia. [So] Source: BMC Res Notes;10(1):106, 2017 Feb 23. [Is] ISSN: 1756-0500 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: In West Africa, penicillin, macrolide and lincosamide resistance among beta-haemolytic streptococci (BHS) isolates has rarely been described. However, such data are critical to detect and track the emergence of antibiotic resistance. METHODS: Beta-haemolytic streptococci were cultured from clinical specimens from patients attending the clinic at the Medical Research Council Unit The Gambia (n = 217) and kept at -70 °C. Of these, 186 were revived and tested for penicillin susceptibility by disc diffusion and E-test methods, and the D-test for determination of constitutive and inducible macrolide-lincosamide (MLS ) resistance phenotypes. RESULTS: The majority of BHS isolates from infections were group A streptococci (GAS) (126/186, 67.7%). Of these, 16% were from invasive disease (30/186). Other BHS isolated included lancefield groups B (19, 10.2%); C (9/186, 4.8%), D (3/186, 1.6%), F (5/186, 2.7%), G (16/186, 8.6%) and non-typeable (8/186, 4.3%). Prevalence of BHS isolated from blood cultures ranges from 0% (2005) to 0.5% (2010). Most (85, 45.7%) of the isolates were from wound infections. Of the 186 BHS isolates, none was resistant to penicillin and 14 (6.1%) were resistant to erythromycin. Of these, 8 (4.3%) demonstrated constitutive MLS resistance, and 5 (2.7%) were inducible MLS resistant. All the inducible MLS isolates were GAS, and majority of the constitutive MLS isolates (6/8, 75.0%) were non-GAS. CONCLUSIONS: Beta-haemolytic streptococci, predominantly GAS are associated with a wide range of infections in The Gambia. It is reassuring that macrolide and lincosamide resistance is relatively low. However, monitoring of MLS resistance is necessary with the global spread of resistant BHS strains. [Mh] Termos MeSH primário: Antibacterianos/farmacologia Farmacorresistência Bacteriana Múltipla Lincosamidas/farmacologia Macrolídeos/farmacologia Streptococcus pyogenes/efeitos dos fármacos Estreptogramina B/farmacologia [Mh] Termos MeSH secundário: Adolescente Adulto Criança Pré-Escolar Feminino Gâmbia/epidemiologia Hemólise Seres Humanos Incidência Lactente Recém-Nascido Masculino Testes de Sensibilidade Microbiana Meia-Idade Infecções Estreptocócicas/epidemiologia Infecções Estreptocócicas/microbiologia Infecções Estreptocócicas/patologia Streptococcus pyogenes/crescimento & desenvolvimento Streptococcus pyogenes/isolamento & purificação Streptococcus pyogenes/patogenicidade [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (Lincosamides); 0 (Macrolides); 3131-03-1 (Streptogramin B) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170922 [Lr] Data última revisão: 170922 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170225 [St] Status: MEDLINE [do] DOI: 10.1186/s13104-017-2427-x

3 / 105

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 27799208 [Au] Autor: Strauss C; Hu Y; Coates A; Perreten V [Ad] Endereço: Institute of Veterinary Bacteriology, University of Bern, Bern, Switzerland. [Ti] Título: A Novel erm(44) Gene Variant from a Human Staphylococcus saprophyticus Isolate Confers Resistance to Macrolides and Lincosamides but Not Streptogramins. [So] Source: Antimicrob Agents Chemother;61(1), 2017 Jan. [Is] ISSN: 1098-6596 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: A novel erm(44) gene variant, erm(44) , has been identified by whole-genome sequencing in a Staphylococcus saprophyticus isolate from the skin of a healthy person. It has the particularity to confer resistance to macrolides and lincosamides but not to streptogramin B when expressed in S. aureus The erm(44) gene resides on a 19,400-bp genomic island which contains phage-associated proteins and is integrated into the chromosome of S. saprophyticus. [Mh] Termos MeSH primário: Lincosamidas/farmacologia Macrolídeos/farmacologia Staphylococcus aureus/efeitos dos fármacos Estreptograminas/farmacologia [Mh] Termos MeSH secundário: Proteínas de Bactérias/genética Proteínas de Bactérias/metabolismo Farmacorresistência Bacteriana Múltipla/genética Ilhas Genômicas/genética Seres Humanos Testes de Sensibilidade Microbiana Staphylococcus/efeitos dos fármacos Staphylococcus/metabolismo Staphylococcus aureus/genética Estreptogramina B/farmacologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Bacterial Proteins); 0 (Lincosamides); 0 (Macrolides); 0 (Streptogramins); 3131-03-1 (Streptogramin B) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170919 [Lr] Data última revisão: 170919 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161102 [St] Status: MEDLINE

4 / 105

MEDLINE

seleciona para imprimir Fotocópia

[PMID]: 27494920 [Au] Autor: Guérin F; Isnard C; Bucquet F; Fines-Guyon M; Giard JC; Burrus V; Cattoir V [Ad] Endereço: Université de Caen Normandie, EA4655 U2RM (équipe 'Antibio-résistance'), Caen, France. [Ti] Título: Novel chromosome-encoded erm(47) determinant responsible for constitutive MLSB resistance in Helcococcus kunzii. [So] Source: J Antimicrob Chemother;71(11):3046-3049, 2016 Nov. [Is] ISSN: 1460-2091 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: OBJECTIVES: The aim of the study was to identify the determinant responsible for erythromycin resistance in Helcococcus kunzii clinical isolate UCN99 and to characterize the genetic support and environment of this novel gene. METHODS: MICs were determined using the broth microdilution method according to EUCAST guidelines. The entire genome sequence of H. kunzii UCN99 was determined using a 454/Roche GS Junior sequencer. The fragment encompassing the new resistance gene and its own promoter was cloned into the pAT29 shuttle vector and the recombinant plasmid pAT29Ωerm(47) was expressed in both Staphylococcus aureus and Streptococcus agalactiae. The transcription start site (TSS) was experimentally determined by 5' RACE-PCR. RESULTS: UCN99 exhibited a constitutive macrolide/lincosamide/streptogramin B (MLS ) resistance phenotype, suggesting the presence of an Erm protein. WGS allowed the identification of a novel gene, named erm(47), encoding a protein sharing 44%-48% amino acid identity with known Erm methylases. In both S. aureus and S. agalactiae, the introduction of pAT29Ωerm(47) conferred a significant increase (≥16-fold) in MICs of all macrolides and lincosamides tested, as well as a 4-fold increase in MICs of quinupristin (streptogramin B), confirming the MLS resistance. The TSS identification revealed the presence of a short leader peptide, potentially implicated in a translational attenuation mechanism. It was also demonstrated that erm(47) was harboured by a 81 kb genomic island integrated into a chromosomal gene. CONCLUSIONS: This is the first description of a novel MLS resistance determinant, named erm(47). The prevalence of this gene among Gram-positive cocci must be further investigated to determine its clinical significance. [Mh] Termos MeSH primário: Antibacterianos/farmacologia Farmacorresistência Bacteriana Firmicutes/efeitos dos fármacos Genes Bacterianos Lincosamidas/farmacologia Macrolídeos/farmacologia Estreptogramina B/farmacologia [Mh] Termos MeSH secundário: Cromossomos Bacterianos Clonagem Molecular DNA Bacteriano/química DNA Bacteriano/genética Firmicutes/genética Expressão Gênica Testes de Sensibilidade Microbiana Regiões Promotoras Genéticas Análise de Sequência de DNA Staphylococcus aureus/efeitos dos fármacos Staphylococcus aureus/genética Streptococcus agalactiae/efeitos dos fármacos Streptococcus agalactiae/genética Sítio de Iniciação de Transcrição [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (DNA, Bacterial); 0 (Lincosamides); 0 (Macrolides); 3131-03-1 (Streptogramin B) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170814 [Lr] Data última revisão: 170814 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160806 [St] Status: MEDLINE

5 / 105

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 27353268 [Au] Autor: Cipcic Paljetak H; Verbanac D; Padovan J; Dominis-Kramaric M; Kelneric Z; Peric M; Banjanac M; Ergovic G; Simon N; Broskey J; Holmes DJ; Erakovic Haber V [Ad] Endereço: GlaxoSmithKline Research Centre Zagreb, Zagreb, Croatia hana.paljetak@mef.hr. [Ti] Título: Macrolones Are a Novel Class of Macrolide Antibiotics Active against Key Resistant Respiratory Pathogens In Vitro and In Vivo. [So] Source: Antimicrob Agents Chemother;60(9):5337-48, 2016 Sep. [Is] ISSN: 1098-6596 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: As we face an alarming increase in bacterial resistance to current antibacterial chemotherapeutics, expanding the available therapeutic arsenal in the fight against resistant bacterial pathogens causing respiratory tract infections is of high importance. The antibacterial potency of macrolones, a novel class of macrolide antibiotics, against key respiratory pathogens was evaluated in vitro and in vivo MIC values against Streptococcus pneumoniae, Streptococcus pyogenes, Staphylococcus aureus, and Haemophilus influenzae strains sensitive to macrolide antibiotics and with defined macrolide resistance mechanisms were determined. The propensity of macrolones to induce the expression of inducible erm genes was tested by the triple-disk method and incubation in the presence of subinhibitory concentrations of compounds. In vivo efficacy was assessed in a murine model of S. pneumoniae-induced pneumonia, and pharmacokinetic (PK) profiles in mice were determined. The in vitro antibacterial profiles of macrolones were superior to those of marketed macrolide antibiotics, including the ketolide telithromycin, and the compounds did not induce the expression of inducible erm genes. They acted as typical protein synthesis inhibitors in an Escherichia coli transcription/translation assay. Macrolones were characterized by low to moderate systemic clearance, a large volume of distribution, a long half-life, and low oral bioavailability. They were highly efficacious in a murine model of pneumonia after intraperitoneal application even against an S. pneumoniae strain with constitutive resistance to macrolide-lincosamide-streptogramin B antibiotics. Macrolones are the class of macrolide antibiotics with an outstanding antibacterial profile and reasonable PK parameters resulting in good in vivo efficacy. [Mh] Termos MeSH primário: Antibacterianos/farmacologia Farmacorresistência Bacteriana/efeitos dos fármacos Macrolídeos/farmacologia Pneumonia Pneumocócica/tratamento farmacológico Inibidores da Síntese de Proteínas/farmacologia Streptococcus pneumoniae/efeitos dos fármacos [Mh] Termos MeSH secundário: Animais Antibacterianos/farmacocinética Proteínas de Bactérias/genética Proteínas de Bactérias/metabolismo Modelos Animais de Doenças Farmacorresistência Bacteriana/genética Escherichia coli/química Haemophilus influenzae/efeitos dos fármacos Haemophilus influenzae/crescimento & desenvolvimento Cetolídeos/farmacologia Lincosamidas/farmacologia Macrolídeos/farmacocinética Masculino Metiltransferases/genética Metiltransferases/metabolismo Camundongos Camundongos Endogâmicos C57BL Pneumonia Pneumocócica/microbiologia Biossíntese de Proteínas/efeitos dos fármacos Inibidores da Síntese de Proteínas/farmacocinética Staphylococcus aureus/efeitos dos fármacos Staphylococcus aureus/crescimento & desenvolvimento Streptococcus pneumoniae/genética Streptococcus pneumoniae/crescimento & desenvolvimento Streptococcus pyogenes/efeitos dos fármacos Streptococcus pyogenes/crescimento & desenvolvimento Estreptogramina B/farmacologia Relação Estrutura-Atividade [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Ketolides); 0 (Lincosamides); 0 (Macrolides); 0 (Protein Synthesis Inhibitors); 3131-03-1 (Streptogramin B); EC 2.1.1.- (Methyltransferases); EC 2.1.1.184 (ErmA protein, Bacteria); KI8H7H19WL (telithromycin) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170915 [Lr] Data última revisão: 170915 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160630 [St] Status: MEDLINE [do] DOI: 10.1128/AAC.00524-16

6 / 105

MEDLINE

seleciona para imprimir Fotocópia Texto completo SciELO Brasil

[PMID]: 27094233 [Au] Autor: Pereira JN; Rabelo MA; Lima JL; Neto AM; Lopes AC; Maciel MA [Ad] Endereço: Department of Tropical Medicine, Universidade Federal de Pernambuco, Recife, PE, Brasil. Electronic address: ju-biomed@hotmail.com. [Ti] Título: Phenotypic and molecular characterization of resistance to macrolides, lincosamides and type B streptogramin of clinical isolates of Staphylococcus spp. of a university hospital in Recife, Pernambuco, Brazil. [So] Source: Braz J Infect Dis;20(3):276-81, 2016 May-Jun. [Is] ISSN: 1678-4391 [Cp] País de publicação: Brazil [La] Idioma: eng [Ab] Resumo: INTRODUCTION: There is a mechanism of macrolide resistance in Staphylococcus spp. which also affects the lincosamides and type B streptogramins characterizing the so-called MLSB resistance, whose expression can be constitutive (cMLSB) or inducible (iMLSB) and is encoded mainly by ermA and ermC genes. The cMLSB resistance is easily detected by susceptibility testing used in the laboratory routine, but iMLSB resistance is not. Therapy with clindamycin in cases of infection with isolated iMLSB resistance may fail. OBJECTIVE: To characterize the phenotypic (occurrence of cMLSB and iMLSB phenotypes) and molecular (occurrence of ermA and ermC genes) profiles of MLSB resistance of clinical isolates of susceptible and methicillin-resistant Staphylococcus aureus and CNS (coagulase-negative Staphylococcus) from patients of a university hospital, in Pernambuco. METHODS: The antimicrobial susceptibility of 103 isolates was determined by the disk diffusion technique in Mueller-Hinton agar followed by oxacillin screening. The iMLSB phenotype was detected by D test. Isolates with cMLSB and iMLSB phenotypes were subjected to polymerase chain reaction (PCR) for the detection of ermA and ermC genes. RESULTS: The cMLSB and iMLSB phenotypes were respectively identified in 39 (37.9%) and five (4.9%) isolates. The iMLSB phenotype was found only in four (10.8%) methicillin-susceptible S. aureus and one (4.5%) methicillin-resistant S. aureus. In the 44 isolates subjected to PCR, four (9.1%) only ermA gene was detected, a lower frequency when compared to only ermC 17 (38.6%) gene and to one (2.3%) isolate presenting both genes. CONCLUSION: In the Staphylococcus spp. analyzed, the ermC gene was found more often than the ermA, although the iMLSB phenotype had been less frequent than the cMLSB. It was important to perform the D test for its detection to guide therapeutic approaches. [Mh] Termos MeSH primário: Farmacorresistência Bacteriana Múltipla/genética Lincosamidas/farmacologia Macrolídeos/farmacologia Staphylococcus/efeitos dos fármacos Staphylococcus/genética Estreptogramina B/farmacologia [Mh] Termos MeSH secundário: Brasil Testes de Sensibilidade a Antimicrobianos por Disco-Difusão Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos Genes Bacterianos/genética Hospitais Universitários Seres Humanos Fenótipo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Lincosamides); 0 (Macrolides); 3131-03-1 (Streptogramin B) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170302 [Lr] Data última revisão: 170302 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160421 [St] Status: MEDLINE

7 / 105

MEDLINE

seleciona para imprimir Fotocópia

[PMID]: 27045297 [Au] Autor: de Matos PD; de Oliveira TL; Cavalcante FS; Ferreira DC; Iorio NL; Pereira EM; Chamon RC; Dos Santos KR [Ad] Endereço: 1 Department of Medical Microbiology, Institute of Microbiology , Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil . [Ti] Título: Molecular Markers of Antimicrobial Resistance in Methicillin-Resistant Staphylococcus aureus SCCmec IV Presenting Different Genetic Backgrounds. [So] Source: Microb Drug Resist;22(8):700-706, 2016 Dec. [Is] ISSN: 1931-8448 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Methicillin-resistant Staphylococcus aureus (MRSA) carrying SCCmec type IV has emerged in hospitals worldwide. The aim of this study was to evaluate phenotypic and molecular characteristics of antimicrobial resistance in MRSA SCCmec IV isolates, presenting different genetic backgrounds, isolated from hospitals in Rio de Janeiro. The antimicrobial resistance of 128 S. aureus type IV isolates from 11 hospitals was characterized by the disk diffusion test and minimum inhibitory concentration (MIC) test. Mutations in parC gene, which encodes ciprofloxacin resistance, and genes associated with macrolide-lincosamide-streptogramin B (MLS ) resistance were also investigated. MRSA isolates belonging to USA400/ST1 (60 isolates), USA800/ST5 (40), USA1100/ST30 (13), and other 11 (15) lineages were mainly resistant to erythromycin (68%), ciprofloxacin (56%), and clindamycin (50%). The highest antimicrobial resistance rates were found among USA400 isolates (p < 0.05). The majority of them (90%) carried only the erm(C) gene and mainly presented two mutation types in the parC gene. The msr(A) gene was most frequently found among USA800 isolates (p < 0.05). Among MRSA type IV isolates from Rio de Janeiro hospitals, multiresistance, including mutations in parC gene, was associated to the USA400/ST1, while the msr(A) gene was associated with USA800/ST5 isolates, highlighting that these lineages could have more potential to persist in a hospital environment. [Mh] Termos MeSH primário: DNA Topoisomerase IV/genética Farmacorresistência Bacteriana Múltipla/genética Regulação Bacteriana da Expressão Gênica Staphylococcus aureus Resistente à Meticilina/genética Metionina Sulfóxido Redutases/genética Metiltransferases/genética [Mh] Termos MeSH secundário: Antibacterianos/farmacologia Técnicas de Tipagem Bacteriana Brasil/epidemiologia DNA Topoisomerase IV/metabolismo Hospitais Seres Humanos Lincosamidas/farmacologia Macrolídeos/farmacologia Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos Staphylococcus aureus Resistente à Meticilina/crescimento & desenvolvimento Staphylococcus aureus Resistente à Meticilina/isolamento & purificação Metionina Sulfóxido Redutases/metabolismo Metiltransferases/metabolismo Testes de Sensibilidade Microbiana Epidemiologia Molecular Mutação Quinolonas/farmacologia Infecções Estafilocócicas/tratamento farmacológico Infecções Estafilocócicas/epidemiologia Infecções Estafilocócicas/microbiologia Estreptogramina B/farmacologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (Lincosamides); 0 (Macrolides); 0 (Quinolones); 3131-03-1 (Streptogramin B); EC 1.8.4.- (Methionine Sulfoxide Reductases); EC 1.8.4.11 (methionine sulfoxide reductase); EC 2.1.1.- (Methyltransferases); EC 2.1.1.230 (rRNA (adenosine-O-2'-)methyltransferase); EC 5.99.1.- (DNA Topoisomerase IV) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170426 [Lr] Data última revisão: 170426 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160406 [St] Status: MEDLINE

8 / 105

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 26604242 [Au] Autor: Florez-Cuadrado D; Ugarte-Ruiz M; Quesada A; Palomo G; Domínguez L; Porrero MC [Ad] Endereço: Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense de Madrid, Madrid, Spain. [Ti] Título: Description of an erm(B)-carrying Campylobacter coli isolate in Europe. [So] Source: J Antimicrob Chemother;71(3):841-3, 2016 Mar. [Is] ISSN: 1460-2091 [Cp] País de publicação: England [La] Idioma: eng [Mh] Termos MeSH primário: Antibacterianos/farmacologia Infecções por Campylobacter/veterinária Campylobacter coli/efeitos dos fármacos Campylobacter coli/genética Farmacorresistência Bacteriana Metiltransferases/genética Doenças das Aves Domésticas/microbiologia [Mh] Termos MeSH secundário: Animais Infecções por Campylobacter/microbiologia Campylobacter coli/isolamento & purificação DNA Bacteriano/química DNA Bacteriano/genética Europa (Continente) Ordem dos Genes Lincosamidas/farmacologia Macrolídeos/farmacologia Testes de Sensibilidade Microbiana Estreptogramina B/farmacologia [Pt] Tipo de publicação: LETTER; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (DNA, Bacterial); 0 (Lincosamides); 0 (Macrolides); 3131-03-1 (Streptogramin B); EC 2.1.1.- (Methyltransferases); EC 2.1.1.230 (rRNA (adenosine-O-2'-)methyltransferase) [Em] Mês de entrada: 1610 [Cu] Atualização por classe: 161230 [Lr] Data última revisão: 161230 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 151126 [St] Status: MEDLINE [do] DOI: 10.1093/jac/dkv383

9 / 105

MEDLINE

seleciona para imprimir Fotocópia PubMed Central Texto completo Texto completo

[PMID]: 26573051 [Au] Autor: Abdollahi S; Ramazanzadeh R; Khiabani ZD; Kalantar E [Ad] Endereço: . atrop_t51@yahoo.com. [Ti] Título: Epidemiological and Inducible Resistance in Coagulase Negative Staphylococci. [So] Source: Glob J Health Sci;8(4):109-19, 2015 Jul 31. [Is] ISSN: 1916-9736 [Cp] País de publicação: Canada [La] Idioma: eng [Ab] Resumo: BACKGROUND & OBJECTIVES: Coagulase negative staphylococci (CNS) are potential pathogens with the increased use of implants in hospitals. Macrolide, lincosamide and streptogramin B (MLSB) are used in the treatment of staphylococcal infections. The aim of this study was to molecular detection of inducible clindamycin resistance and genetic pattern in CNS isolates and their transmission between hospitals. MATERIALS & METHODS: 110 CNS strains, isolated from hospitalized patients in the intensive care unit and infectious wards of Besat and Toohid hospitals, Sanandaj. Methicillin resistance was done by agar screen test and the resistance inducible Clindamycin by the D-Test. Multiplex PCR was performed, using primers specific for erm (A, B, C, and TR) genes. Diversity of strains was determined by ERIC-PCR technique based on the similarities between DNA fingerprints by using Jaccards coefficient in the SAHN program of the NTSYS-pc software. RESULTS: Of the 110 isolates, 64(58.2%) were methicillin -resistant CNS (MRCNS), 48(43.6%) were resistant to erythromycin (ERCNS). Out of 48 Erythromycin-resistant strains 5 (10.4%) were iMLS B phenotypes that 4 isolates showed genes erm by Multiplex PCR. The ERIC-PCR profiles allowed typing of the 110 isolates into 90 ERIC-types which were grouped into fourteen main clusters (C1-C14). CONCLUSION: The results of this study also showed that most of CNS isolated produced different genomic fingerprint patterns, therefore, source of infection is differen t. [Mh] Termos MeSH primário: Antibacterianos/farmacologia Clindamicina/farmacologia Infecções Estafilocócicas/tratamento farmacológico Infecções Estafilocócicas/microbiologia Staphylococcus/efeitos dos fármacos [Mh] Termos MeSH secundário: Coagulase Infecção Hospitalar/microbiologia Impressões Digitais de DNA Farmacorresistência Bacteriana Múltipla Seres Humanos Unidades de Terapia Intensiva Irã (Geográfico)/epidemiologia Lincosamidas/farmacologia Macrolídeos/farmacologia Staphylococcus aureus Resistente à Meticilina Testes de Sensibilidade Microbiana Fenótipo Reação em Cadeia da Polimerase Staphylococcus/classificação Staphylococcus/isolamento & purificação Estreptogramina B/farmacologia [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (Coagulase); 0 (Lincosamides); 0 (Macrolides); 3131-03-1 (Streptogramin B); 3U02EL437C (Clindamycin) [Em] Mês de entrada: 1606 [Cu] Atualização por classe: 170220 [Lr] Data última revisão: 170220 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 151118 [St] Status: MEDLINE [do] DOI: 10.5539/gjhs.v8n4p109

10 / 105

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 26377866 [Au] Autor: Anastasi E; Giguère S; Berghaus LJ; Hondalus MK; Willingham-Lane JM; MacArthur I; Cohen ND; Roberts MC; Vazquez-Boland JA [Ad] Endereço: Microbial Pathogenesis Unit, School of Biomedical Sciences and The Roslin Institute, University of Edinburgh, Edinburgh, UK. [Ti] Título: Novel transferable erm(46) determinant responsible for emerging macrolide resistance in Rhodococcus equi. [So] Source: J Antimicrob Chemother;70(12):3184-90, 2015 Dec. [Is] ISSN: 1460-2091 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: OBJECTIVES: The objective of this study was to identify the molecular mechanism of macrolide resistance in the actinomycete Rhodococcus equi, a major equine pathogen and zoonotic agent causing opportunistic infections in people. METHODS: Macrolide-resistant (n = 62) and macrolide-susceptible (n = 62) clinical isolates of R. equi from foals in the USA were studied. WGS of 18 macrolide-resistant and 6 macrolide-susceptible R. equi was performed. Representative sequences of all known macrolide resistance genes identified to date were used to search the genome assemblies for putative homologues. PCR was used to screen for the presence of the identified resistance determinant in the rest of the isolates. Mating experiments were performed to verify mobility of the gene. RESULTS: A novel erm gene, erm(46), was identified in all sequenced resistant isolates, but not in susceptible isolates. There was complete association between macrolide resistance and the presence of erm(46) as detected by PCR screening of all 124 clinical isolates of R. equi. Expression of erm(46) in a macrolide-susceptible strain of R. equi induced high-level resistance to macrolides, lincosamides and streptogramins B, but not to other classes of antimicrobial agents. Transfer of erm(46) to macrolide-susceptible R. equi was confirmed. The transfer frequency ranged from 3 × 10(-3) to 1 × 10(-2). CONCLUSIONS: This is the first molecular characterization of resistance to macrolides, lincosamides and streptogramins B in R. equi. Resistance was due to the presence of a novel erm(46) gene mobilizable likely by conjugation, which has spread among equine isolates of R. equi in the USA. [Mh] Termos MeSH primário: Antibacterianos/farmacologia Farmacorresistência Bacteriana Transferência Genética Horizontal Genes Bacterianos Macrolídeos/farmacologia Rhodococcus equi/efeitos dos fármacos Rhodococcus equi/genética [Mh] Termos MeSH secundário: Infecções por Actinomycetales/microbiologia Infecções por Actinomycetales/veterinária Animais Animais Recém-Nascidos Conjugação Genética DNA Bacteriano/química DNA Bacteriano/genética Doenças dos Cavalos/microbiologia Cavalos Lincosamidas/farmacologia Rhodococcus equi/isolamento & purificação Análise de Sequência de DNA Estreptogramina B/farmacologia Estados Unidos [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (DNA, Bacterial); 0 (Lincosamides); 0 (Macrolides); 3131-03-1 (Streptogramin B) [Em] Mês de entrada: 1608 [Cu] Atualização por classe: 170308 [Lr] Data última revisão: 170308 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150918 [St] Status: MEDLINE [do] DOI: 10.1093/jac/dkv279

---

página 1 de 11

ir para página

Refinar a pesquisa

Base de dados : MEDLINE

Formulário avançado

		Pesquisar	no campo
1			PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde