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[PMID]:28415016
[Au] Autor:Duelge KJ; Nishshanka U; De Alwis HG
[Ad] Endereço:Food and Drug Administration, Center for Veterinary Medicine, Office of Research, 8401 Muirkirk Road, Laurel, MD 20708, USA.
[Ti] Título:An LC-MS/MS method for the determination of antibiotic residues in distillers grains.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1053:81-86, 2017 May 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Antibiotics are used in ethanol production to discourage the growth of bacteria that would result in lower ethanol content and a lower quality product. A survey conducted by the FDA (FY 2010 Nationwide Survey of Distillers Grains for Antibiotic Residues, 2009 [1]) revealed that the residues of these antibiotics can remain in the distillers grains (DG) by-product, which is used as an animal feed ingredient. The low levels of antibiotic residues in DG could be a public health concern, as they could lead to antimicrobial resistance. To enable the quantitative determination of these antibiotics (erythromycin, penicillin G, virginiamycin M1 and virginiamycin S1), we developed a sensitive LC-MS/MS method. The residues were extracted from distillers grains with a mixture of acetonitrile and buffer followed by acetonitrile. The combined extract was diluted with water and washed with hexane. An aliquot was cleaned up on an Oasis HLB solid phase extraction cartridge. Extracts were analyzed by LC-tandem mass spectrometry. The method was successfully validated using a variety of different matrices such as corn DG, corn & milo DG, and deoiled corn DG. Absolute recoveries of the analytes ranged from 53 to 106%. Accuracy ranged from 90 to 101% based on calibration by matrix standards. The limits of quantitation and relative standard deviation were all satisfactory to support future surveillance studies.
[Mh] Termos MeSH primário: Ração Animal/análise
Antibacterianos/análise
Eritromicina/análise
Penicilina G/análise
Estreptogramina A/análise
Estreptogramina Grupo B/análise
Espectrometria de Massas em Tandem/métodos
Virginiamicina/análise
[Mh] Termos MeSH secundário: Acetonitrilos/química
Animais
Cromatografia Líquida/métodos
Grãos Comestíveis/química
Hexanos/química
Limite de Detecção
Extração em Fase Sólida/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Acetonitriles); 0 (Anti-Bacterial Agents); 0 (Hexanes); 0 (Streptogramin Group B); 0 (virginiamycin factor S1); 11006-76-1 (Virginiamycin); 2DDG612ED8 (n-hexane); 63937KV33D (Erythromycin); 8W4UOL59AZ (Streptogramin A); Q42T66VG0C (Penicillin G); Z072SB282N (acetonitrile)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170418
[St] Status:MEDLINE


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[PMID]:28260587
[Au] Autor:Carvalho I; Campo RD; Sousa M; Silva N; Carrola J; Marinho C; Santos T; Carvalho S; Nóvoa M; Quaresma M; Pereira JE; Cobo M; Igrejas G; Poeta P
[Ad] Endereço:1​Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal 2​Department of Genetics and Biotechnology, Vila Real, Portugal 3​Functional Genomics and Proteomics Unit, Vila Real, Portugal.
[Ti] Título:Antimicrobial-resistant Escherichia coli and Enterococcus spp. isolated from Miranda donkey (Equus asinus): an old problem from a new source with a different approach.
[So] Source:J Med Microbiol;66(2):191-202, 2017 Mar.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: The Miranda donkey (Equus asinus) is an endangeredasinine from Miranda do Douro region, located in the north east of Portugal. We studied the antimicrobial resistance and virulence genes in Escherichia coli and Enterococcus spp. isolates from these animals. METHODOLOGY: In March 2014, a total of 66 faecal samples were recovered from independent animals. Antibiotic resistance was determined by the disc diffusion method. Carriage of genes coding for antibiotic-resistant and virulent factors was analysed by PCR. RESULTS: A total of 66 E. coli and 41 enterococcal isolates were detected, with Enterococcus faecium (61 %) and Enterococcus hirae (24 %) being the most prevalent species. For enterococcal isolates, high percentages of resistance rates to tetracycline (68.3 %), quinupristin/dalfopristin (51.2 %) and ciprofloxacin (48.8 %) were observed. The genes erm(A) and/or erm(B), tet(M) and/or tet(L), vat(D) and/or vat(E) and aph(3')-IIIa were also found. The most frequent virulence gene detected was gel(E), followed by ace, cpd and hyl. Escherichia coli isolates were highly resistant to streptomycin (78 %), whereas 39 % of them exhibited resistance to aminoglycosides and tetracycline. Genes sul1 and/or sul2 were detected in 66.7 % of trimethoprim/sulfamethoxazole-resistant isolates. The virulence genes detected were fim(A) (46 %) and cnf1 (27 %). CONCLUSION: To the best of our knowledge, this is the first report showing antibiotic resistance among Escherichiacoli and Enterococcus spp. isolates from the Miranda donkey in Portugal, indicating possible antibiotic-resistant bacterial reservoirs. However, the detection of these resistances presents a low risk for other animals and human beings in that rural area.
[Mh] Termos MeSH primário: Farmacorresistência Bacteriana Múltipla
Enterococcus/genética
Equidae/microbiologia
Escherichia coli/genética
[Mh] Termos MeSH secundário: Aminoglicosídeos/farmacologia
Animais
Antibacterianos/farmacologia
Proteínas de Bactérias/genética
Ciprofloxacino/farmacologia
DNA Bacteriano/genética
Enterococcus/classificação
Enterococcus/isolamento & purificação
Escherichia coli/classificação
Escherichia coli/isolamento & purificação
Canamicina Quinase/genética
Metiltransferases/genética
Testes de Sensibilidade Microbiana
Portugal
Sulfametoxazol/farmacologia
Tetraciclina/farmacologia
Trimetoprima/farmacologia
Virginiamicina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aminoglycosides); 0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (DNA, Bacterial); 11006-76-1 (Virginiamycin); 126602-89-9 (quinupristin-dalfopristin); 5E8K9I0O4U (Ciprofloxacin); AN164J8Y0X (Trimethoprim); EC 2.1.1.- (Methyltransferases); EC 2.1.1.184 (ErmA protein, Bacteria); EC 2.7.1.95 (Kanamycin Kinase); F8VB5M810T (Tetracycline); JE42381TNV (Sulfamethoxazole)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170321
[Lr] Data última revisão:
170321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170307
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000423


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[PMID]:28222110
[Au] Autor:Costa MC; Bessegatto JA; Alfieri AA; Weese JS; Filho JA; Oba A
[Ad] Endereço:Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.
[Ti] Título:Different antibiotic growth promoters induce specific changes in the cecal microbiota membership of broiler chicken.
[So] Source:PLoS One;12(2):e0171642, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Antimicrobials are sometimes given to food animals at low doses in order to promote faster growth. However, the mechanisms by which those drugs improve performance are not fully understood. This study aimed to investigate the impact of zinc bacitracin (55g/ton), enramycin (10g/ton); halquinol® (30g/ton); virginiamycin (16,5g/ton) and avilamycin (10g/ton) on the cecal microbiota of broiler chicken, compared to a control group. Six hundred and twenty four chicks (Cobb 500) arriving to an experimental unit were randomly assigned into each treatment with four repetitions per treatment. The cecal content of 16 animals per treatment (n = 96) was used for DNA extraction and sequencing of the V4 region of the 16S rRNA gene using Illumina technology. The use of antimicrobials induced significant changes in membership but not in structure of the cecal microbiota compared to the control group, suggesting a greater impact on the less abundant species of bacteria present in that environment. Halquinol was the only drug that did not affect microbial membership. Firmicutes comprised the major bacterial phylum present in the cecum of all groups. There was no statistical difference in relative abundances of the main phyla between treated animals and the control group (all P>0.05). Treatment with enramycin was associated with decreased richness and with lower relative abundance of unclassified Firmicutes, Clostridium XI, unclassified Peptostreptococcaceae (all P<0.001) and greater abundance of Clostridium XIVb (P = 0.004) and Anaerosporobacter spp. (P = 0.015), and treatment with bacitracin with greater relative abundance of Bilophila spp. (P = 0.004). Several bacterial genera were identified as representative of usage of each drug. This study used high throughput sequencing to characterize the impact of several antimicrobials in broiler chicken under controlled conditions and add new insights to the current knowledge on how AGPs affect the cecal microbiota of chicken.
[Mh] Termos MeSH primário: Ração Animal
Antibacterianos/farmacologia
Ceco/microbiologia
Galinhas/microbiologia
Aditivos Alimentares/farmacologia
Microbioma Gastrointestinal/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Antibacterianos/administração & dosagem
Bacitracina/farmacologia
Bactérias/classificação
Bactérias/efeitos dos fármacos
Galinhas/crescimento & desenvolvimento
Cloroquinolinóis/farmacologia
Variação Genética
Oligossacarídeos/farmacologia
Peptídeos/farmacologia
Análise de Componente Principal
Distribuição Aleatória
Ribotipagem
Virginiamicina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Chloroquinolinols); 0 (Food Additives); 0 (Oligosaccharides); 0 (Peptides); 11006-76-1 (Virginiamycin); 1405-87-4 (Bacitracin); 4GP7CLG9EG (enramycin); 720WDX56D3 (avilamycin); Z7Z4BX535U (halquinol)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170222
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0171642


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[PMID]:27255566
[Au] Autor:Naghi Shokri A; Ghasemi HA; Taherpour K
[Ad] Endereço:Department of Animal Science, Faculty of Agriculture, Ilam University, Ilam, Iran.
[Ti] Título:Evaluation of Aloe vera and synbiotic as antibiotic growth promoter substitutions on performance, gut morphology, immune responses and blood constitutes of broiler chickens.
[So] Source:Anim Sci J;88(2):306-313, 2017 Feb.
[Is] ISSN:1740-0929
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:This study was performed to investigate the effect of dietary supplementation with Aloe vera (AV) powder and synbiotic as growth promoter agents on performance, gut morphology, immune responses, hematology and serum biochemistry in broilers. A total of 240-day-old male broiler chicks (Ross 308) were randomly assigned to six treatments with four replicates. Birds were offered either a corn-soybean meal basal diet (control) or the basal diet supplemented with 200 mg/kg virginiamycin (VM), 1 g/kg synbiotic (Syn), 2.5 g/kg AV (AV1), 5.0 g/kg AV (AV2) or 7.5 g/kg AV (AV3). Chickens fed any of the diets, except diet AV1, exhibited better feed conversion ratios at the 14-28 day period and higher average daily gain and duodenal villus height/crypt depth ratio at 42 days than those fed the control diet. Synbiotic supplementation caused a marked increase in the serum antibody titer against infectious bursal disease and infectious bronchitis vaccines. Feeding diet AV3 significantly increased red blood cell count and hemoglobin concentration, and decreased serum triglyceride level compared to the control group. The results suggested that dietary inclusion of 5 and 7.5 g/kg AV, similar with synbiotic supplementation, can be applied as effective alternatives to in-feed antibiotics for broiler diets.
[Mh] Termos MeSH primário: Aloe
Ração Animal
Fenômenos Fisiológicos da Nutrição Animal/fisiologia
Anticorpos/sangue
Galinhas/crescimento & desenvolvimento
Galinhas/imunologia
Dieta/veterinária
Suplementos Nutricionais
Duodeno/anatomia & histologia
Simbióticos/administração & dosagem
Virginiamicina/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Galinhas/anatomia & histologia
Galinhas/sangue
Contagem de Eritrócitos
Hemoglobinas
Masculino
Pós
Feijão de Soja
Triglicerídeos/sangue
Ganho de Peso
Zea mays
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies); 0 (Hemoglobins); 0 (Powders); 0 (Triglycerides); 11006-76-1 (Virginiamycin)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170316
[Lr] Data última revisão:
170316
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160604
[St] Status:MEDLINE
[do] DOI:10.1111/asj.12629


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[PMID]:27898865
[Au] Autor:Lemos BJ; Castro FG; Santos LS; Mendonça BP; Couto VR; Fernandes JJ
[Ti] Título:Monensin, virginiamycin, and flavomycin in a no-roughage finishing diet fed to zebu cattle.
[So] Source:J Anim Sci;94(10):4307-4314, 2016 Oct.
[Is] ISSN:1525-3163
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Two experiments were designed to evaluate the effects of monensin, virginiamycin, and flavomycin on growth performance, carcass characteristics, apparent total tract nutrient digestibility, and rumen fermentation of zebu cattle fed a no-roughage finishing diet (whole shelled corn [WSC] based). In Exp. 1, 100 crossbred bulls (; 392 kg [SD 46.8] average initial BW) were blocked by initial BW in a 101-d feedlot trial. Five treatments were evaluated using 4 pens per treatment (5 bulls/pen): monensin at 30 mg/kg DM, virginiamycin at 25 mg/kg DM, monensin at 20 mg/kg DM plus virginiamycin at 25 mg/kg DM, flavomycin at 4.4 mg/kg DM, and monensin at 20 mg/kg DM plus flavomycin at 2.2 mg/kg DM. There were no differences in growth performance (final BW, ADG, DMI, and G:F; ≥ 0.527) and carcass characteristics (HCW, dressing percent, and 12th-rib fat; ≥ 0.235) among treatments. In Exp. 2, 7 ruminally fistulated steers were used in a 7 × 7 Latin square design to evaluate the 5 treatments of Exp. 1 and 2 additional treatments: monensin at 30 mg/kg DM plus virginiamycin at 25 mg/kg DM and monensin at 20 mg/kg DM plus flavomycin at 4.4 mg/kg DM. Experimental periods were 14 d in length (9 d of adaptation and 5 d of measurements). Apparent total tract DM, OM, CP, and NDF digestibilities were similar among treatments ( ≥ 0.224). There was no treatment effect ( ≥ 0.253) in rumen fermentation responses (ruminal pH, rumen ammonia nitrogen, VFA, and number of protozoa). In conclusion, no evidence of benefits to cattle fed a no-roughage WSC-based diet was found to support the use of monensin combined with virginiamycin or flavomycin in the doses tested herein.
[Mh] Termos MeSH primário: Bambermicinas/farmacologia
Bovinos
Dieta/veterinária
Fibras na Dieta
Monensin/farmacologia
Virginiamicina/farmacologia
[Mh] Termos MeSH secundário: Ração Animal/análise
Fenômenos Fisiológicos da Nutrição Animal
Animais
Antibacterianos/farmacologia
Composição Corporal/efeitos dos fármacos
Digestão/fisiologia
Fermentação
Trato Gastrointestinal/efeitos dos fármacos
Masculino
Inibidores da Síntese de Proteínas/farmacologia
Ionóforos de Próton/farmacologia
Rúmen/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Dietary Fiber); 0 (Protein Synthesis Inhibitors); 0 (Proton Ionophores); 11006-76-1 (Virginiamycin); 11015-37-5 (Bambermycins); 906O0YJ6ZP (Monensin)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161130
[St] Status:MEDLINE
[do] DOI:10.2527/jas.2016-0504


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[PMID]:27898843
[Au] Autor:Salinas-Chavira J; Barreras A; Plascencia A; Montano MF; Navarrete JD; Torrentera N; Zinn RA
[Ti] Título:Influence of protein nutrition and virginiamycin supplementation on feedlot growth performance and digestive function of calf-fed Holstein steers.
[So] Source:J Anim Sci;94(10):4276-4286, 2016 Oct.
[Is] ISSN:1525-3163
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Two experiments were conducted to examine the influence of protein and virginiamycin (VM) supplementation on feedlot growth performance, digestion, and metabolizable AA (MAA) supply of calf-fed Holstein steers. Growth performance and dietary energetics were evaluated in 120 Holstein steers (127 ± 9 kg). During the initial 112-d feeding period, a steam-flaked corn-based diet was balanced to meet either 100% (MAB) or 87% (UREA) of MAA requirements. Diets were supplemented with or without 22.5 mg/kg VM in a 2 × 2 factorial arrangement. Subsequently (d 112 to 308), all steers received the UREA diet with or without VM. During the initial 112-d, MAB increased ADG, G:F, and dietary NE ( < 0.01). Thereafter, when all steers received the UREA diet, ADG, G:F, and dietary NE were not different ( > 0.10) across initial supplementation treatments. Overall (d 1 to 308), MAB did not affect ADG ( > 0.10) but enhanced G:F efficiency ( = 0.03) and dietary NE ( = 0.05). During the initial 112-d period and through the remainder of the experiment, VM increased G:F ( < 0.01) and dietary NE ( < 0.01). Four Holstein steers (146 ± 4 kg) with cannulas in the rumen and proximal duodenum were used in a 4 × 4 Latin square design to evaluate initial 112-d treatment effects on digestive function. There were no treatment effects ( > 0.10) on ruminal digestion of OM, NDF, starch, microbial efficiency, or total tract digestion of OM and NDF. The MAB increased indispensable AA flow to the small intestine ( < 0.01) and total tract digestion of N ( < 0.01) and starch ( = 0.04). Observed AA supply to small intestine was in agreement with expected supply ( = 0.96). Virginiamycin decreased ( = 0.04) nonammonia N flow to the small intestine and did not affect ( > 0.10) total tract N digestion. Extrapolating from AA supplies in the metabolism study, MAB satisfied indispensable AA requirements during the initial 112-d period, whereas the UREA diet met 73.5% and 79.2% of methionine and lysine requirements, respectively. During the subsequent periods (d 112 to 308) indispensable AA supplies exceeded theoretical requirements. We conclude that enhancements in energy utilization when diets are balanced to meet MAA requirements of calf-fed Holstein steers during the initial 112-d feedlot period remain appreciable throughout time on feed. Virginiamycin enhanced efficiency of energy utilization throughout the feedlot growing-finishing period.
[Mh] Termos MeSH primário: Ração Animal/análise
Bovinos/crescimento & desenvolvimento
Proteínas na Dieta/farmacologia
Digestão/efeitos dos fármacos
Virginiamicina/farmacologia
[Mh] Termos MeSH secundário: Fenômenos Fisiológicos da Nutrição Animal
Animais
Bovinos/metabolismo
Dieta/veterinária
Proteínas na Dieta/administração & dosagem
Suplementos Nutricionais
Masculino
Estado Nutricional
Inibidores da Síntese de Proteínas/farmacologia
Amido/metabolismo
Virginiamicina/administração & dosagem
Ganho de Peso
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Dietary Proteins); 0 (Protein Synthesis Inhibitors); 11006-76-1 (Virginiamycin); 9005-25-8 (Starch)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161130
[St] Status:MEDLINE
[do] DOI:10.2527/jas.2016-0576


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[PMID]:27769188
[Au] Autor:Wang S; Guo Y; Lv J; Qi X; Li D; Chen Z; Zhang X; Wang L; Yu F
[Ad] Endereço:Department of Laboratory Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
[Ti] Título:Characteristic of Enterococcus faecium clinical isolates with quinupristin/dalfopristin resistance in China.
[So] Source:BMC Microbiol;16(1):246, 2016 Oct 21.
[Is] ISSN:1471-2180
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Quinupristin/dalfopristin (Q/D) is a valuable alternative antibiotic to vancomycin for the treatment of multi-drug resistant Enterococcus faecium infections. However, resistance to Q/D in E. faecium clinical isolates and nosocomial dissemination of Q/D-resistant E. faecium have been reported in several countries and should be of concern. RESULTS: From January 2012 to December 2015, 911 E. faecium clinical isolates were isolated from various specimens of inpatients at the first Affiliated Hospital of Wenzhou Medical University located in Wenzhou, east China. Of 911 E. faecium clinical isolates, 9 (1.0 %, 9/911) were resistant to Q/D, with the Q/D MIC values of 64 mg/L(1), 32 mg/L(1), 16 mg/L(3), 8 mg/L(1) and 4 mg/L(3) determined by broth microdilution. All Q/D-resistant isolates were susceptible to vancomycin, tigecycline and teicoplanin but resistant to penicillin, ampicillin and erythromycin. vatE was only found in one Q/D-resistant E. faecium isolate while vatD was not detected in any of the isolates tested. 8 of 9 Q/D-resistant E. faecium isolates were found be positive for both ermB and msrC. The combinations of Q/D resistance determinants were ermB-msrC (7 isolates) and ermB-msrC-vatE (one isolate). ST78, ST761, ST94, ST21 and ST323 accounted for 4, 2, 1, 1 and 1 isolate, respectively, among which ST78 was the prevalent ST. CONCLUSION: Q/D-resistant E. faecium clinical isolates were first described in China. Carriage of vatE, ermB and msrC was responsible for Q/D resistance.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Enterococcus faecium/efeitos dos fármacos
Enterococcus faecium/isolamento & purificação
Infecções por Bactérias Gram-Positivas/microbiologia
Virginiamicina/farmacologia
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
China/epidemiologia
Infecção Hospitalar
DNA Bacteriano/genética
Farmacorresistência Bacteriana Múltipla
Enterococcus faecium/genética
Genes Bacterianos
Infecções por Bactérias Gram-Positivas/epidemiologia
Seres Humanos
Testes de Sensibilidade Microbiana
Tipagem de Sequências Multilocus/métodos
Prevalência
RNA Ribossômico 16S/genética
Vancomicina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); 11006-76-1 (Virginiamycin); 126602-89-9 (quinupristin-dalfopristin); 6Q205EH1VU (Vancomycin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170608
[Lr] Data última revisão:
170608
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE


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[PMID]:27528592
[Au] Autor:Yan X; Li Z; Chlebowicz MA; Tao X; Ni M; Hu Y; Li Z; Grundmann H; Murray S; Pascoe B; Sheppard SK; Bo X; Dijl JM; Du P; Zhang M; You Y; Yu X; Meng F; Wang S; Zhang J
[Ad] Endereço:State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Chin
[Ti] Título:Genetic features of livestock-associated Staphylococcus aureus ST9 isolates from Chinese pigs that carry the lsa(E) gene for quinupristin/dalfopristin resistance.
[So] Source:Int J Med Microbiol;306(8):722-729, 2016 Dec.
[Is] ISSN:1618-0607
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Whole-genome sequencing (WGS) was used to investigate the genetic features of the recently identified lsa(E) gene in porcine S. aureus ST9 isolates. Three quinupristin/dalfopristin-resistant isolates harboring the lsa(E) gene (two MRSA and one MSSA) were sequenced. Phylogenetic analysis of 184S. aureus genomes showed that ST9 porcine isolates belong to a distinct sequence cluster. Further analysis showed that all isolates were deficient in the recently described type IV restriction-modification system and SCCmec type XII was identified in the two MRSA isolates, which included a rare class C2 mec gene complex. A 24kb ΨSCC fragment was found in the MRSA and MSSA isolates sharing 99% nucleotide sequence homology with the ΨSCCJCSC6690 (O-2) element of a ST9 MRSA isolate from Thailand (accession number AB705453). Comparison of these ST9 isolates with 181 publically available S. aureus genomes identified 24 genes present in all (100%) ST9 isolates, that were absent from the most closely related human isolate. Our analysis suggests that the sequenced quinupristin/dalfopristin-resistant ST9 lineage represent a reservoir of mobile genetic elements associated with resistance and virulence features.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Farmacorresistência Bacteriana/genética
Genes Bacterianos
Staphylococcus aureus/genética
Staphylococcus aureus/isolamento & purificação
Suínos/microbiologia
Virginiamicina/farmacologia
[Mh] Termos MeSH secundário: Animais
Análise por Conglomerados
Enzimas de Restrição-Modificação do DNA/deficiência
DNA Bacteriano/química
DNA Bacteriano/genética
Ordem dos Genes
Genoma Bacteriano
Genótipo
Sequências Repetitivas Dispersas
Tipagem Molecular
Filogenia
Análise de Sequência de DNA
Homologia de Sequência
Staphylococcus aureus/classificação
Tailândia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (DNA Restriction-Modification Enzymes); 0 (DNA, Bacterial); 11006-76-1 (Virginiamycin); 126602-89-9 (quinupristin-dalfopristin)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160817
[St] Status:MEDLINE


  9 / 1174 MEDLINE  
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[PMID]:27038946
[Au] Autor:Bischoff KM; Zhang Y; Rich JO
[Ad] Endereço:U.S. Department of Agriculture, Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, 1815 N. University St., Peoria, IL, 61604, USA. kenneth.bischoff@ars.usda.gov.
[Ti] Título:Fate of virginiamycin through the fuel ethanol production process.
[So] Source:World J Microbiol Biotechnol;32(5):76, 2016 May.
[Is] ISSN:1573-0972
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Antibiotics are frequently used to prevent and treat bacterial contamination of commercial fuel ethanol fermentations, but there is concern that antibiotic residues may persist in the distillers grains coproducts. A study to evaluate the fate of virginiamycin during the ethanol production process was conducted in the pilot plant facilities at the National Corn to Ethanol Research Center, Edwardsville, IL. Three 15,000-liter fermentor runs were performed: one with no antibiotic (F1), one dosed with 2 parts per million (ppm) of a commercial virginiamycin product (F2), and one dosed at 20 ppm of virginiamycin product (F3). Fermentor samples, distillers dried grains with solubles (DDGS), and process intermediates (whole stillage, thin stillage, syrup, and wet cake) were collected from each run and analyzed for virginiamycin M and virginiamycin S using a liquid chromatography-mass spectrometry method. Virginiamycin M was detected in all process intermediates of the F3 run. On a dry-weight basis, virginiamycin M concentrations decreased approximately 97 %, from 41 µg/g in the fermentor to 1.4 µg/g in the DDGS. Using a disc plate bioassay, antibiotic activity was detected in DDGS from both the F2 and F3 runs, with values of 0.69 µg virginiamycin equivalent/g sample and 8.9 µg/g, respectively. No antibiotic activity (<0.6 µg/g) was detected in any of the F1 samples or in the fermentor and process intermediate samples from the F2 run. These results demonstrate that low concentrations of biologically active antibiotic may persist in distillers grains coproducts produced from fermentations treated with virginiamycin.
[Mh] Termos MeSH primário: Antibacterianos/metabolismo
Biocombustíveis/análise
Etanol/metabolismo
Saccharomyces cerevisiae/metabolismo
Virginiamicina/metabolismo
Zea mays/metabolismo
[Mh] Termos MeSH secundário: Antibacterianos/análise
Cromatografia Líquida
Etanol/análise
Fermentação
Espectrometria de Massas
Virginiamicina/análise
Zea mays/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Biofuels); 11006-76-1 (Virginiamycin); 3K9958V90M (Ethanol)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160404
[St] Status:MEDLINE
[do] DOI:10.1007/s11274-016-2026-3


  10 / 1174 MEDLINE  
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[PMID]:26982529
[Au] Autor:Dorival J; Annaval T; Risser F; Collin S; Roblin P; Jacob C; Gruez A; Chagot B; Weissman KJ
[Ad] Endereço:UMR 7365, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), CNRS-Université de Lorraine, Biopôle de l'Université de Lorraine , Campus Biologie Santé, 9 Avenue de la Forêt de Haye, CS 50184, 54505 VandÅ“uvre-lès-Nancy CEDEX, France.
[Ti] Título:Characterization of Intersubunit Communication in the Virginiamycin trans-Acyl Transferase Polyketide Synthase.
[So] Source:J Am Chem Soc;138(12):4155-67, 2016 Mar 30.
[Is] ISSN:1520-5126
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Modular polyketide synthases (PKSs) direct the biosynthesis of clinically valuable secondary metabolites in bacteria. The fidelity of chain growth depends on specific recognition between successive subunits in each assembly line: interactions mediated by C- and N-terminal "docking domains" (DDs). We have identified a new family of DDs in trans-acyl transferase PKSs, exemplified by a matched pair from the virginiamycin (Vir) system. In the absence of C-terminal partner (VirA (C)DD) or a downstream catalytic domain, the N-terminal DD (VirFG (N)DD) exhibits multiple characteristics of an intrinsically disordered protein. Fusion of the two docking domains results in a stable fold for VirFG (N)DD and an overall protein-protein complex of unique topology whose structure we support by site-directed mutagenesis. Furthermore, using small-angle X-ray scattering (SAXS), the positions of the flanking acyl carrier protein and ketosynthase domains have been identified, allowing modeling of the complete intersubunit interface.
[Mh] Termos MeSH primário: Aciltransferases/metabolismo
Policetídeo Sintases/metabolismo
Virginiamicina/química
[Mh] Termos MeSH secundário: Espectroscopia de Ressonância Magnética
Modelos Moleculares
Virginiamicina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
11006-76-1 (Virginiamycin); 79956-01-7 (Polyketide Synthases); EC 2.3.- (Acyltransferases)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170314
[Lr] Data última revisão:
170314
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160317
[St] Status:MEDLINE
[do] DOI:10.1021/jacs.5b13372



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