Base de dados : MEDLINE
Pesquisa : D04.345.566.875.250 [Categoria DeCS]
Referências encontradas : 58 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 6 ir para página                

  1 / 58 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:21768509
[Au] Autor:Akbergenov R; Shcherbakov D; Matt T; Duscha S; Meyer M; Wilson DN; Böttger EC
[Ad] Endereço:Institut für Medizinische Mikrobiologie, Universität Zürich, Gloriastrasse 30/32, 8006 Zürich, Switzerland.
[Ti] Título:Molecular basis for the selectivity of antituberculosis compounds capreomycin and viomycin.
[So] Source:Antimicrob Agents Chemother;55(10):4712-7, 2011 Oct.
[Is] ISSN:1098-6596
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Capreomycin and the structurally similar compound viomycin are cyclic peptide antibiotics which are particularly active against Mycobacterium tuberculosis, including multidrug resistant strains. Both antibiotics bind across the ribosomal interface involving 23S rRNA helix 69 (H69) and 16S rRNA helix 44 (h44). The binding site of tuberactinomycins in h44 partially overlaps with that of aminoglycosides, and they share with these drugs the side effect of irreversible hearing loss. Here we studied the drug target interaction on ribosomes modified by site-directed mutagenesis. We identified rRNA residues in h44 as the main determinants of phylogenetic selectivity, predict compensatory evolution to impact future resistance development, and propose mechanisms involved in tuberactinomycin ototoxicity, which may enable the development of improved, less-toxic derivatives.
[Mh] Termos MeSH primário: Antituberculosos/farmacologia
Capreomicina/farmacologia
Mycobacterium tuberculosis/efeitos dos fármacos
Ribossomos/efeitos dos fármacos
Viomicina/farmacologia
[Mh] Termos MeSH secundário: Aminoglicosídeos/farmacologia
Antituberculosos/metabolismo
Antituberculosos/toxicidade
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Capreomicina/metabolismo
Capreomicina/toxicidade
Farmacorresistência Bacteriana Múltipla/genética
Enviomicina/análogos & derivados
Enviomicina/farmacologia
Enviomicina/toxicidade
Mutagênese Sítio-Dirigida
Mycobacterium tuberculosis/genética
Mycobacterium tuberculosis/metabolismo
RNA Ribossômico 16S/metabolismo
RNA Ribossômico 23S/metabolismo
Viomicina/metabolismo
Viomicina/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Aminoglycosides); 0 (Antitubercular Agents); 0 (Bacterial Proteins); 0 (RNA, Ribosomal, 16S); 0 (RNA, Ribosomal, 23S); 11003-38-6 (Capreomycin); 85B3A97YJY (tuberactinomycin); XU299C23A2 (Enviomycin); YVU35998K5 (Viomycin)
[Em] Mês de entrada:1203
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110720
[St] Status:MEDLINE
[do] DOI:10.1128/AAC.00628-11


  2 / 58 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:19863583
[Au] Autor:Chevin LM; Lande R
[Ad] Endereço:Division of Biology, Imperial College London, Silwood Park Campus, Ascot, Berkshire, SL5 7PY, United Kingdom. l.chevin@imperial.ac.uk
[Ti] Título:When do adaptive plasticity and genetic evolution prevent extinction of a density-regulated population?
[So] Source:Evolution;64(4):1143-50, 2010 Apr 01.
[Is] ISSN:1558-5646
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We study the dynamics of evolutionary recovery after an abrupt environmental shift in a density-regulated population with evolving plasticity. Maladaptation to the new environment initially causes the population to decline, until adaptive phenotypic plasticity and genetic evolution restore positive population growth rate. We assume that selection on a quantitative trait is density-independent and that the initial cost of plasticity is much lower than the benefit of the initial plastic response. The initial partially adaptive plasticity reduces the effective magnitude of the environmental shift, whereas evolution of plasticity increases the rate of adaptation. Both effects greatly facilitate population persistence. In contrast, density dependence of population growth always hinders persistence. With theta-logistic population regulation, a lower value of theta produces a faster initial population decline and a higher extinction risk.
[Mh] Termos MeSH primário: Adaptação Biológica
Evolução Molecular
Extinção Biológica
Modelos Genéticos
[Mh] Termos MeSH secundário: Enviomicina
Densidade Demográfica
Dinâmica Populacional
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
XU299C23A2 (Enviomycin)
[Em] Mês de entrada:1007
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:091030
[St] Status:MEDLINE
[do] DOI:10.1111/j.1558-5646.2009.00875.x


  3 / 58 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:15541892
[Au] Autor:Erb S; Lopak V; Smith C
[Ad] Endereço:Centre for the Neurobiology of Stress, Department of Life Science and Psychology, University of Toronto at Scarborough, 1265 Military Trail, Scarborough, Ontario, M1C 1A4 Canada. erb@utsc.utoronto.ca
[Ti] Título:Cocaine pre-exposure produces a sensitized and context-specific c-fos mRNA response to footshock stress in the central nucleus of the AMYGDALA.
[So] Source:Neuroscience;129(3):719-25, 2004.
[Is] ISSN:0306-4522
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In recent years, there has been growing interest in the putative relationship between stress and vulnerability to relapse in former drug addicts. In animal studies aimed at exploring this relationship, it has been shown that brief exposure to intermittent footshock stress produces reliable reinstatement of drug seeking after prolonged drug-free periods. Whereas footshock reinstates drug seeking, it does not reinstate behaviors maintained by non-drug reinforcers, suggesting that prior drug experience may produce a form of sensitization within neuronal systems that mediate stress-induced reinstatement. The primary objective of the present experiments was to determine whether pre-exposure to cocaine produces a long-lasting, sensitized neuronal response to footshock stress within two brain regions known to mediate footshock-induced reinstatement; the central nucleus of the amygdala (CeA) and bed nucleus of the stria terminalis (BNST). In experiment 1, animals were injected for 7 days with cocaine (days 1 and 7 in test chambers; days 2-6 in homecages) or saline. After 21 drug-free days, they were exposed to footshock or no footshock. In experiment 2, rats were injected daily for 7 days with cocaine in one of two contexts and saline in the alternate context. After 21 drug-free days, they were given footshock either in the same context that they were given cocaine in or the alternate context. In CeA, footshock produced enhanced expression of c-fos mRNA in cocaine, but not saline, pre-exposed animals. Furthermore, this effect was gated by the environmental context in which cocaine was given; footshock only enhanced c-fos mRNA expression when it was given in a context that had previously been paired with cocaine. Although footshock induced c-fos mRNA expression in the BNST, its effects in this region were not dependent on drug history. The major findings are that a history of cocaine exposure produces sensitization to an acute stressor within CeA, and this effect is gated by environmental context.
[Mh] Termos MeSH primário: Tonsila do Cerebelo/efeitos dos fármacos
Cocaína/farmacologia
Proteínas Proto-Oncogênicas c-fos/metabolismo
Estresse Fisiológico/metabolismo
[Mh] Termos MeSH secundário: Tonsila do Cerebelo/metabolismo
Tonsila do Cerebelo/efeitos da radiação
Análise de Variância
Animais
Comportamento Animal
Diagnóstico por Imagem/métodos
Inibidores da Captação de Dopamina/farmacologia
Eletrochoque/efeitos adversos
Enviomicina
Regulação da Expressão Gênica/efeitos dos fármacos
Regulação da Expressão Gênica/efeitos da radiação
Hibridização In Situ/métodos
Masculino
Proteínas Proto-Oncogênicas c-fos/genética
RNA Mensageiro/metabolismo
Ratos
Ratos Long-Evans
Núcleos Septais/efeitos dos fármacos
Núcleos Septais/metabolismo
Núcleos Septais/efeitos da radiação
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Dopamine Uptake Inhibitors); 0 (Proto-Oncogene Proteins c-fos); 0 (RNA, Messenger); I5Y540LHVR (Cocaine); XU299C23A2 (Enviomycin)
[Em] Mês de entrada:0503
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:041116
[St] Status:MEDLINE


  4 / 58 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
[PMID]:12936980
[Au] Autor:Thomas MG; Chan YA; Ozanick SG
[Ad] Endereço:Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA. thomas@bact.wisc.edu
[Ti] Título:Deciphering tuberactinomycin biosynthesis: isolation, sequencing, and annotation of the viomycin biosynthetic gene cluster.
[So] Source:Antimicrob Agents Chemother;47(9):2823-30, 2003 Sep.
[Is] ISSN:0066-4804
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The tuberactinomycin antibiotics are essential components in the drug arsenal against Mycobacterium tuberculosis infections and are specifically used for the treatment of multidrug-resistant tuberculosis. These antibiotics are also being investigated for their targeting of the catalytic RNAs involved in viral replication and for the treatment of bacterial infections caused by methicillin-resistant Staphylococcus aureus strains and vancomycin-resistant enterococci. We report on the isolation, sequencing, and annotation of the biosynthetic gene cluster for one member of this antibiotic family, viomycin, from Streptomyces sp. strain ATCC 11861. This is the first gene cluster for a member of the tuberactinomycin family of antibiotics sequenced, and the information gained can be extrapolated to all members of this family. The gene cluster covers 36.3 kb of DNA and encodes 20 open reading frames that we propose are involved in the biosynthesis, regulation, export, and activation of viomycin, in addition to self-resistance to the antibiotic. These results enable us to predict the metabolic logic of tuberactinomycin production and begin steps toward the combinatorial biosynthesis of these antibiotics to complement existing chemical modification techniques to produce novel tuberactinomycin derivatives.
[Mh] Termos MeSH primário: Antibióticos Antituberculose/biossíntese
Arginina/análogos & derivados
Enviomicina/análogos & derivados
Enviomicina/biossíntese
Família Multigênica/genética
Streptomyces/genética
Streptomyces/metabolismo
Viomicina/biossíntese
[Mh] Termos MeSH secundário: Aminoácidos/biossíntese
Antibióticos Antituberculose/isolamento & purificação
Arginina/biossíntese
Cromatografia Líquida de Alta Pressão
Cosmídeos/genética
DNA Bacteriano/genética
Biblioteca Gênica
Lisina/biossíntese
Oligopeptídeos/biossíntese
Viomicina/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amino Acids); 0 (Antibiotics, Antitubercular); 0 (DNA, Bacterial); 0 (Oligopeptides); 0 (capreomycidine); 85B3A97YJY (tuberactinomycin); 94ZLA3W45F (Arginine); K3Z4F929H6 (Lysine); XU299C23A2 (Enviomycin); YVU35998K5 (Viomycin)
[Em] Mês de entrada:0310
[Cu] Atualização por classe:170219
[Lr] Data última revisão:
170219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:030826
[St] Status:MEDLINE


  5 / 58 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:11135553
[Au] Autor:Jenne A; Hartig JS; Piganeau N; Tauer A; Samarsky DA; Green MR; Davies J; Famulok M
[Ad] Endereço:Kekulé-Institut für Organische Chemie und Biochemie, Gerhard-Domagk-Str. 1, 35121, Bonn, Germany.
[Ti] Título:Rapid identification and characterization of hammerhead-ribozyme inhibitors using fluorescence-based technology.
[So] Source:Nat Biotechnol;19(1):56-61, 2001 Jan.
[Is] ISSN:1087-0156
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The ability to rapidly identify small molecules that interact with RNA would have significant clinical and research applications. Low-molecular-weight molecules that bind to RNA have the potential to be used as drugs. Therefore, technologies facilitating the rapid and reliable identification of such activities become increasingly important. We have applied a fluorescence-based assay to screen for modulators of hammerhead ribozyme (HHR) catalysis from a small library of antibiotic compounds. Several unknown potent inhibitors of the hammerhead cleavage reaction were identified and further characterized. Tuberactinomycin A, for which positive cooperativity of inhibition in vitro was found, also reduced ribozyme cleavage in vivo. The assay is applicable to the screening of mixtures of compounds, as inhibitory activities were detected within a collection of 2,000 extracts from different actinomycete strains. This approach allows the rapid, reliable, and convenient identification and characterization of ribozyme modulators leading to insights difficult to obtain by classical methodology.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Enviomicina/análogos & derivados
RNA Catalítico/química
RNA Catalítico/metabolismo
[Mh] Termos MeSH secundário: Sequência de Bases
Catálise
Desenho de Drogas
Avaliação Pré-Clínica de Medicamentos/métodos
Enviomicina/farmacologia
Cinética
Conformação de Ácido Nucleico
RNA Catalítico/antagonistas & inibidores
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (RNA, Catalytic); 85B3A97YJY (tuberactinomycin); XU299C23A2 (Enviomycin)
[Em] Mês de entrada:0102
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:010103
[St] Status:MEDLINE


  6 / 58 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:11372389
[Au] Autor:He G; Xiao H
[Ti] Título:[Recent advance of anti-tuberculous agents].
[So] Source:Zhonghua Jie He He Hu Xi Za Zhi;23(10):621-5, 2000 Oct.
[Is] ISSN:1001-0939
[Cp] País de publicação:China
[La] Idioma:chi
[Mh] Termos MeSH primário: Anti-Infecciosos
Antituberculosos
Rifamicinas
[Mh] Termos MeSH secundário: Aminoglicosídeos
Antibacterianos
Enviomicina
Fluoroquinolonas
Lactamas
Macrolídeos
Nitroimidazóis
Fenazinas
Fenotiazinas
Tioacetazona/análogos & derivados
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Aminoglycosides); 0 (Anti-Bacterial Agents); 0 (Anti-Infective Agents); 0 (Antitubercular Agents); 0 (Fluoroquinolones); 0 (Lactams); 0 (Macrolides); 0 (Nitroimidazoles); 0 (Phenazines); 0 (Phenothiazines); 0 (Rifamycins); MMG78X7SSR (Thioacetazone); XU299C23A2 (Enviomycin)
[Em] Mês de entrada:0209
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:010525
[St] Status:MEDLINE


  7 / 58 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:9315086
[Au] Autor:Morse BK; Brown MS; Gagne JW; McArthur HA; McCormick EL; Murphy TK; Narrol MH; Perry DA; Smogowicz AA; Wax RG; Wong JW
[Ad] Endereço:Central Research Division, Pfizer Inc., Groton, Connecticut 06340, USA.
[Ti] Título:Production of tuberactinamine A by Streptomyces griseoverticillatus var. tuberacticus NRRL 3482 fed with (S)-2-aminoethyl-L-cysteine.
[So] Source:J Antibiot (Tokyo);50(8):698-700, 1997 Aug.
[Is] ISSN:0021-8820
[Cp] País de publicação:Japan
[La] Idioma:eng
[Mh] Termos MeSH primário: Enviomicina/análogos & derivados
[Mh] Termos MeSH secundário: Cisteína/análogos & derivados
Cisteína/farmacologia
Enviomicina/biossíntese
Fermentação
Inibidores da Síntese de Proteínas/farmacologia
Streptomyces/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Protein Synthesis Inhibitors); 0 (tuberactinamine A); 2936-69-8 (S-2-aminoethyl cysteine); K848JZ4886 (Cysteine); XU299C23A2 (Enviomycin)
[Em] Mês de entrada:9710
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:970801
[St] Status:MEDLINE


  8 / 58 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:9195872
[Au] Autor:Wallis MG; Streicher B; Wank H; von Ahsen U; Clodi E; Wallace ST; Famulok M; Schroeder R
[Ad] Endereço:Institute of Microbiology and Genetics University of Vienna Dr. Bohrgasse 9, A-1030, Vienna, Austria.
[Ti] Título:In vitro selection of a viomycin-binding RNA pseudoknot.
[So] Source:Chem Biol;4(5):357-66, 1997 May.
[Is] ISSN:1074-5521
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The peptide antibiotic viomycin inhibits ribosomal protein synthesis, group I intron self-splicing and self-cleavage of the human hepatitis delta virus ribozyme. To understand the molecular basis of RNA binding and recognition by viomycin, we isolated a variety of novel viomycin-binding RNA molecules using in vitro selection. RESULTS: More than 90% of the selected RNA molecules shared one continuous highly conserved region of 14 nucleotides. Mutational analyses, structural probing, together with footprinting experiments by chemical modification, and Pb2+-induced cleavage showed that this conserved sequence harbours the antibiotic-binding site and forms a stem-loop structure. Moreover, the loop is engaged in a long-range interaction forming a pseudoknot. CONCLUSIONS: A comparison between the novel viomycin-binding motif and the natural RNA target sites for viomycin showed that all these segments form a pseudoknot at the antibiotic-binding site. We therefore conclude that this peptide antibiotic has a strong selectivity for particular RNA pseudoknots.
[Mh] Termos MeSH primário: Antibacterianos/metabolismo
Conformação de Ácido Nucleico
RNA/química
RNA/metabolismo
Viomicina/metabolismo
[Mh] Termos MeSH secundário: Sequência de Bases
Sítios de Ligação
Clonagem Molecular
Sequência Conservada
Enviomicina/análogos & derivados
Enviomicina/metabolismo
Seres Humanos
Chumbo/química
Dados de Sequência Molecular
Mutação
RNA/isolamento & purificação
Sondas RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (RNA Probes); 2P299V784P (Lead); 63231-63-0 (RNA); 85B3A97YJY (tuberactinomycin); XU299C23A2 (Enviomycin); YVU35998K5 (Viomycin)
[Em] Mês de entrada:9708
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:970501
[St] Status:MEDLINE


  9 / 58 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:8613991
[Au] Autor:Wank H; Schroeder R
[Ad] Endereço:Institute of Microbiology and Genetics, University of Vienna, Vienna Biocenter, Austria.
[Ti] Título:Antibiotic-induced oligomerisation of group I intron RNA.
[So] Source:J Mol Biol;258(1):53-61, 1996 Apr 26.
[Is] ISSN:0022-2836
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Antibiotics act as inhibitors of various biological processes. Here we demonstrate that some tuberactinomycins, hitherto known as inhibitors of prokaryotic protein synthesis and of group I intron self-splicing, have a modulatory effect on group I intron RNAs. The linear intron, which is excised during the self-splicing process, is still an active molecular capable of performing an intramolecular transesterification resulting in a circular molecule. However, in the presence of sub-inhibitory concentrations of tuberactinomycins, the intron reacts intermolecularly leading to the formation of linear head-to-tail intron-oligomers. The antibiotic stimulates the intron to react in trans instead of in cis. The phage T4-derived td intron uses the same sites for oligomerisation as for circularisation. Gel- retardation experiments demonstrate that the intron RNA forms non-covalent complexes in the presence of the antibiotic. It might be envisaged that the role of these peptide antibiotics is to bridge RNA molecules mediating RNA-RNA interactions and thus enabling their reaction. The tuberactinomycins are further able to induce the interaction of heterologous introns. The ligation of the T4 phage-derived td intron with the Tetrahymena rRNA intron is very efficient, resulting in molecules composed of two introns derived from different species. The td intron attacks the Tetrahymena intron at various sites, which are located within double-stranded regions. These observations suggest that small molecules like these basic peptide antibiotics could have mediated RNA-RNA interactions in a pre-protein era.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Íntrons/efeitos dos fármacos
Conformação de Ácido Nucleico/efeitos dos fármacos
RNA Catalítico/efeitos dos fármacos
Viomicina/farmacologia
[Mh] Termos MeSH secundário: Animais
Bacteriófago T4/química
Sequência de Bases
Enviomicina/análogos & derivados
Enviomicina/farmacologia
Dados de Sequência Molecular
RNA/química
Processamento de RNA/efeitos dos fármacos
RNA Catalítico/química
RNA de Protozoário/química
RNA Ribossômico/química
RNA Viral/química
Análise de Sequência de DNA
Tetrahymena thermophila/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (RNA, Catalytic); 0 (RNA, Protozoan); 0 (RNA, Ribosomal); 0 (RNA, Viral); 0 (RNA, circular); 63231-63-0 (RNA); 85B3A97YJY (tuberactinomycin); XU299C23A2 (Enviomycin); YVU35998K5 (Viomycin)
[Em] Mês de entrada:9606
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:960426
[St] Status:MEDLINE


  10 / 58 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
[PMID]:7621836
[Au] Autor:Olive JE; De Abreu DM; Rastogi T; Andersen AA; Mittermaier AK; Beattie TL; Collins RA
[Ad] Endereço:Canadian Institute for Advanced Research Program in Evolutionary Biology, Department of Molecular and Medical Genetics, University of Toronto, Ontario.
[Ti] Título:Enhancement of Neurospora VS ribozyme cleavage by tuberactinomycin antibiotics.
[So] Source:EMBO J;14(13):3247-51, 1995 Jul 03.
[Is] ISSN:0261-4189
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Several examples of inhibition of the function of a ribozyme or RNA-protein complex have shown that certain antibiotics can interact specifically with RNA. There are, however, few examples of antibiotics that have a positive, rather than a negative, effect on the function of an RNA. We have found that micromolar concentrations of viomycin, a basic, cyclic peptide antibiotic of the tuberactinomycin group, enhance the cleavage of a ribozyme derived from Neurospora VS RNA. Viomycin decreases by an order of magnitude the concentration of magnesium required for cleavage. It also stimulates an otherwise insignificant transcleavage reaction by enhancing interactions between RNA molecules. The ability of viomycin to enhance some RNA-mediated reactions but inhibit others, including translation and Group I intron splicing, demonstrates the potential for natural selection by small molecules during evolution in the 'RNA world' and may have broader implications with respect to ribozyme expression and activity in contemporary cells.
[Mh] Termos MeSH primário: Enviomicina/análogos & derivados
Neurospora/efeitos dos fármacos
RNA Catalítico/efeitos dos fármacos
RNA Fúngico/efeitos dos fármacos
[Mh] Termos MeSH secundário: Enviomicina/farmacologia
Cloreto de Magnésio/metabolismo
Modelos Moleculares
Neurospora/genética
Conformação de Ácido Nucleico
Processamento Pós-Transcricional do RNA
RNA Catalítico/genética
RNA Catalítico/metabolismo
RNA Fúngico/genética
RNA Fúngico/metabolismo
Viomicina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Catalytic); 0 (RNA, Fungal); 02F3473H9O (Magnesium Chloride); 85B3A97YJY (tuberactinomycin); XU299C23A2 (Enviomycin); YVU35998K5 (Viomycin)
[Em] Mês de entrada:9508
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:950703
[St] Status:MEDLINE



página 1 de 6 ir para página                
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde