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Teixeira, Mauro Martins
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[PMID]:29287781
[Au] Autor:Cervantes-Llanos M; Lagumersindez-Denis N; Marín-Prida J; Pavón-Fuentes N; Falcon-Cama V; Piniella-Matamoros B; Camacho-Rodríguez H; Fernández-Massó JR; Valenzuela-Silva C; Raíces-Cruz I; Pentón-Arias E; Teixeira MM; Pentón-Rol G
[Ad] Endereço:Center for Genetic Engineering and Biotechnology (CIGB), Ave. 31 e/ 158 y 190, Cubanacán, Playa, Havana, PO Box 6162, Cuba.
[Ti] Título:Beneficial effects of oral administration of C-Phycocyanin and Phycocyanobilin in rodent models of experimental autoimmune encephalomyelitis.
[So] Source:Life Sci;194:130-138, 2018 Feb 01.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The only three oral treatments currently available for multiple sclerosis (MS) target the relapsing forms of the disease and concerns regarding efficacy, safety and tolerability limit their use. Identifying novel oral disease-modifying therapies for MS, targeting both its inflammatory and neurodegenerative components is still a major goal. AIM: The scope of this study was to provide evidence that the oral administration of C-Phycocyanin (C-PC), the main biliprotein of the Spirulina platensis cyanobacteria and its tetrapyrrolic prosthetic group, Phycocyanobilin (PCB), exert ameliorating actions on rodent models of experimental autoimmune encephalomyelitis (EAE). MAIN METHODS: EAE was induced in Lewis rats using the spinal cord encephalitogen from Sprague Dawley rats and in C57BL6 mice with MOG peptide. Clinical signs, motor function, oxidative stress markers, cytokine levels by ELISA and transmission electron microscopy analysis were assessed. KEY FINDINGS: Either prophylactic or early therapeutic administration of C-PC to Lewis rats with EAE, significantly improved clinical signs and restored the motor function of the animals. Furthermore, C-PC positively modulated oxidative stress markers measured in brain homogenate and serum and protected the integrity of cerebral myelin sheaths as shown by transmission electron microscopy analysis. In C57BL/6 mice with EAE, PCB orally improved clinical status of the animals and reduced the expression levels of brain IL-6 and IFN-γ proinflammatory cytokines. SIGNIFICANCE: These results, for the first time, support the fact that both C-PC and PCB administered orally could potentially improve neuroinflammation, protect from demyelination and axonal loss, which may be translated into an improved quality of life for MS patients.
[Mh] Termos MeSH primário: Encéfalo/efeitos dos fármacos
Encefalomielite Autoimune Experimental/tratamento farmacológico
Fármacos Neuroprotetores/uso terapêutico
Ficobilinas/uso terapêutico
Ficocianina/uso terapêutico
[Mh] Termos MeSH secundário: Administração Oral
Animais
Anti-Inflamatórios/administração & dosagem
Anti-Inflamatórios/química
Anti-Inflamatórios/uso terapêutico
Encéfalo/patologia
Citocinas/análise
Modelos Animais de Doenças
Encefalomielite Autoimune Experimental/patologia
Feminino
Interleucina-6/análise
Masculino
Camundongos Endogâmicos C57BL
Fármacos Neuroprotetores/administração & dosagem
Fármacos Neuroprotetores/química
Ficobilinas/administração & dosagem
Ficobilinas/química
Ficocianina/administração & dosagem
Ficocianina/química
Ratos Endogâmicos Lew
Ratos Sprague-Dawley
Spirulina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Cytokines); 0 (Interleukin-6); 0 (Neuroprotective Agents); 0 (Phycobilins); 11016-15-2 (Phycocyanin); 36NUT04V2K (phycocyanobilin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171231
[St] Status:MEDLINE


  2 / 1086 MEDLINE  
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[PMID]:27773238
[Au] Autor:Liu C; Cao Z; Wang J; Sun Z; He S; Chen W
[Ad] Endereço:Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing 210098, China; College of Environment, Hohai University, Nanjing 210098, China.
[Ti] Título:Performance and mechanism of phycocyanin removal from water by low-frequency ultrasound treatment.
[So] Source:Ultrason Sonochem;34:214-221, 2017 01.
[Is] ISSN:1873-2828
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Ultrasonication pretreatment of raw water with high content of algal cells might cause an increase in dissolved organic nitrogen (DON) and proteins, which must be removed effectively before coagulation. In this study, the efficiency of sonication treatment in removing typical proteins derived from algal cells was investigated by applying ultrasonic waves at 20, 40, 60, 80, and 100kHz, and the influencing factors and removal mechanism were discussed. The results showed that low-frequency sonication could degrade phycocyanin to some extent, achieving about 95% removal rate after 150min of sonication. However, excitation emission matrix analysis indicated that ultrasonication could not entirely degrade phycocyanin into inorganic nitrogen, and many proteins and nitrogen-containing organics were found in the samples after sonication. While the total nitrogen concentration remained consistent during the entire sonication process (240min), the total inorganic nitrogen concentration increased from 0.6 to 1.3mg/L, indicating that only 33.3% of DON was oxidized into inorganic nitrogen. Nevertheless, sonication could significantly attenuate the interference of phycocyanin in coagulation and enhance coagulation. The mechanical effects and free-radical oxidation resulting from cavitation collapse could be responsible for the degradation of phycocyanin and proteins following sonication. In all, the use of ultrasonic treatment as a posttreatment following sonication to remove algal cells from raw water may not be beneficial.
[Mh] Termos MeSH primário: Ficocianina/isolamento & purificação
Sonicação
Poluentes Químicos da Água/isolamento & purificação
Purificação da Água/métodos
Água/química
[Mh] Termos MeSH secundário: Água Potável/química
Ficocianina/química
Poluentes Químicos da Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Drinking Water); 0 (Water Pollutants, Chemical); 059QF0KO0R (Water); 11016-15-2 (Phycocyanin)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180131
[Lr] Data última revisão:
180131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:29101856
[Au] Autor:Tan S; Tan X; Chi Z; Zhang D; Li W
[Ad] Endereço:Department of Environmental Engineering, Harbin Institute of Technology, Weihai, 2# Wenhua West Road, Weihai 264209, PR China.
[Ti] Título:In vitro assessment of the toxicity of lead (Pb ) to phycocyanin.
[So] Source:Chemosphere;192:171-177, 2018 Feb.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This work reports the influence of lead (Pb ) on fluorescence characteristics and protein structure of phycocyanin molecules experimentally in vitro. The fluorescence intensity decreases with the increasing concentration of Pb from 0 to 5 × 10 mol L , showing the fluorescence quenching of phycocyanin by Pb . The quenching process is suggested to be static regarding the calculation results and the experimental results of time-resolved fluorescence decay profiles. The synchronous fluorescence spectra show that the effect of Pb on the Tyr residues of phycocyanin is more significant than the Trp residues. The forming of aggregation by the interaction of Pb with phycocyanin molecules is suggested from the results of resonance light scattering spectra. The UV-Vis spectra of the protein skeleton of phycocyanin have a red-shift of about 10 nm with increasing the Pb concentration from 0 to 5 × 10 mol L , indicating a change in the protein skeleton and its secondary structure. With the increasing Pb concentration, the two negative peaks (209 nm and 218 nm) on circular dichroism spectra become smaller, showing a decrease of the α-helix structure. These results may give people a deeper understanding of that how the heavy metal (Pb ) can affect the chemo-physical properties of phycocyanin.
[Mh] Termos MeSH primário: Chumbo/química
Ficocianina/química
[Mh] Termos MeSH secundário: Dicroísmo Circular
Chumbo/toxicidade
Estrutura Secundária de Proteína
Espectrometria de Fluorescência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
11016-15-2 (Phycocyanin); 2P299V784P (Lead)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180123
[Lr] Data última revisão:
180123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171105
[St] Status:MEDLINE


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[PMID]:28873634
[Au] Autor:Falkeborg MF; Roda-Serrat MC; Burnæs KL; Nielsen ALD
[Ad] Endereço:Centre for Food Technology, Danish Technological Institute, Kongsvang alle 29, DK-8000 Aarhus C, Denmark. Electronic address: mfa@dti.dk.
[Ti] Título:Stabilising phycocyanin by anionic micelles.
[So] Source:Food Chem;239:771-780, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phycocyanins are pigment-protein complexes with potential application as natural food colourants. The perceived colour of phycocyanins varies with pH, and a method to stabilise the colour over a broad range of pH values is requested by the food industry. In this work, the stabilising effect of sodium dodecyl sulphate (SDS) micelles on pH-induced colour variations of phycocyanin was examined. SDS was shown to stabilise the blue conformation of phycocyanin, preventing formation of the green conformation, which is prevalent at low pH. The studies indicated that the stabilising effect occurred through interaction or entrapment of the non-protonated, circular helical (blue) structure of phycocyanin and the anionic SDS micelles. The interaction prevented conversion into protonated, partially unfolded (green) phycocyanin species. This information opens for new possibilities to stabilise the blue conformation of phycocyanin and to apply the stabilised form in food products as a natural blue food colourant.
[Mh] Termos MeSH primário: Ficocianina/química
[Mh] Termos MeSH secundário: Ânions
Micelas
Dodecilsulfato de Sódio
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anions); 0 (Micelles); 11016-15-2 (Phycocyanin); 368GB5141J (Sodium Dodecyl Sulfate)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


  5 / 1086 MEDLINE  
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[PMID]:28911120
[Au] Autor:Hochrein L; Machens F; Messerschmidt K; Mueller-Roeber B
[Ad] Endereço:University of Potsdam, Cell2Fab Research Unit, Karl-Liebknecht-Str. 24-25, 14476 Potsdam, Germany.
[Ti] Título:PhiReX: a programmable and red light-regulated protein expression switch for yeast.
[So] Source:Nucleic Acids Res;45(15):9193-9205, 2017 Sep 06.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Highly regulated induction systems enabling dose-dependent and reversible fine-tuning of protein expression output are beneficial for engineering complex biosynthetic pathways. To address this, we developed PhiReX, a novel red/far-red light-regulated protein expression system for use in Saccharomyces cerevisiae. PhiReX is based on the combination of a customizable synTALE DNA-binding domain, the VP64 activation domain and the light-sensitive dimerization of the photoreceptor PhyB and its interacting partner PIF3 from Arabidopsis thaliana. Robust gene expression and high protein levels are achieved by combining genome integrated red light-sensing components with an episomal high-copy reporter construct. The gene of interest as well as the synTALE DNA-binding domain can be easily exchanged, allowing the flexible regulation of any desired gene by targeting endogenous or heterologous promoter regions. To allow low-cost induction of gene expression for industrial fermentation processes, we engineered yeast to endogenously produce the chromophore required for the effective dimerization of PhyB and PIF3. Time course experiments demonstrate high-level induction over a period of at least 48 h.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/genética
Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética
Proteínas de Ligação a DNA/genética
Engenharia Genética/métodos
Proteínas de Homeodomínio/genética
Fitocromo B/genética
Saccharomyces cerevisiae/genética
[Mh] Termos MeSH secundário: Arabidopsis/química
Arabidopsis/genética
Arabidopsis/metabolismo
Proteínas de Arabidopsis/metabolismo
Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo
Proteínas de Ligação a DNA/metabolismo
Expressão Gênica
Vetores Genéticos/química
Vetores Genéticos/metabolismo
Proteínas de Fluorescência Verde/genética
Proteínas de Fluorescência Verde/metabolismo
Proteínas de Homeodomínio/metabolismo
Luz
Transdução de Sinal Luminoso
Ficobilinas/biossíntese
Ficobilinas/genética
Ficocianina/biossíntese
Ficocianina/genética
Fitocromo B/metabolismo
Plasmídeos/química
Plasmídeos/metabolismo
Regiões Promotoras Genéticas
Multimerização Proteica
Saccharomyces cerevisiae/metabolismo
Saccharomyces cerevisiae/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Basic Helix-Loop-Helix Transcription Factors); 0 (DNA-Binding Proteins); 0 (Homeodomain Proteins); 0 (PHYB protein, Arabidopsis); 0 (PIF3 protein, Arabidopsis); 0 (Phycobilins); 0 (enhanced green fluorescent protein); 11016-15-2 (Phycocyanin); 136250-22-1 (Phytochrome B); 147336-22-9 (Green Fluorescent Proteins); 36NUT04V2K (phycocyanobilin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx610


  6 / 1086 MEDLINE  
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[PMID]:28601364
[Au] Autor:Kong F; Zhang M; Chen J; Fan L; Xiao H; Liu S; Cao C
[Ad] Endereço:Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
[Ti] Título:Continuous protein concentration via free-flow moving reaction boundary electrophoresis.
[So] Source:J Chromatogr A;1508:169-175, 2017 Jul 28.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this work, we developed the model and theory of free-flow moving reaction boundary electrophoresis (FFMRB) for continuous protein concentration for the first time. The theoretical results indicated that (i) the moving reaction boundary (MRB) can be quantitatively designed in free-flow electrophoresis (FFE) system; (ii) charge-to-mass ratio (Z/M) analysis could provide guidance for protein concentration optimization; and (iii) the maximum processing capacity could be predicted. To demonstrate the model and theory, three model proteins of hemoglobin (Hb), cytochrome C (Cyt C) and C-phycocyanin (C-PC) were chosen for the experiments. The experimental results verified that (i) stable MRBs with different velocities could be established in FFE apparatus with weak acid/weak base neutralization reaction system; (ii) proteins of Hb, Cyt C and C-PC were well concentrated with FFMRB; and (iii) a maximum processing capacity and recovery ratio of Cyt C enrichment were 126mL/h and 95.5% respectively, and a maximum enrichment factor was achieved 12.6 times for Hb. All of the experiments demonstrated the protein concentration model and theory. In contrast to other methods, the continuous processing ability enables FFMRB to efficiently enrich diluted protein or peptide in large volume solution.
[Mh] Termos MeSH primário: Citocromos c/análise
Eletroforese/métodos
Hemoglobinas/análise
Ficocianina/análise
[Mh] Termos MeSH secundário: Eletroforese/instrumentação
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hemoglobins); 11016-15-2 (Phycocyanin); 9007-43-6 (Cytochromes c)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170612
[St] Status:MEDLINE


  7 / 1086 MEDLINE  
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[PMID]:28576442
[Au] Autor:Nozue S; Katayama M; Terazima M; Kumazaki S
[Ad] Endereço:Department of Chemistry, Graduate School of Science, Kyoto University, Japan.
[Ti] Título:Comparative study of thylakoid membranes in terminal heterocysts and vegetative cells from two cyanobacteria, Rivularia M-261 and Anabaena variabilis, by fluorescence and absorption spectral microscopy.
[So] Source:Biochim Biophys Acta;1858(9):742-749, 2017 09.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Heterocyst is a nitrogen-fixing cell differentiated from a cell for oxygen-evolving photosynthesis (vegetative cell) in some filamentous cyanobacteria when fixed nitrogen (e.g., ammonia and nitrate) is limited. Heterocysts appear at multiple separated positions in a single filament with an interval of 10-20 cells in some genera (including Anabaena variabilis). In other genera, a single heterocyst appears only at the basal terminal in a filament (including Rivularia M-261). Such morphological diversity may necessitate different properties of heterocysts. However, possible differences in heterocysts have largely remained unexplored due to the minority of heterocysts among major vegetative cells. Here, we have applied spectroscopic microscopy to Rivularia and A. variabilis to analyze their thylakoid membranes in individual cells. Absorption and fluorescence spectral imaging enabled us to estimate concentrations and interconnections of key photosynthetic components like photosystem I (PSI), photosystem II (PSII) and subunits of light-harvesting phycobilisome including phycocyanin (PC). The concentration of PC in heterocysts of Rivularia is far higher than that of A. variabilis. Fluorescence quantum yield of PC in Rivularia heterocysts was found to be virtually the same as those in its vegetative cells, while fluorescence quantum yield of PC in A. variabilis heterocysts was enhanced in comparison with its vegetative cells. PSI concentration in the thylakoid membranes of heterocysts seems to remain nearly the same as those of the vegetative cells in both the species. The average stoichiometric ratio between PSI monomer and PC hexamer in Rivularia heterocysts is estimated to be about 1:1.
[Mh] Termos MeSH primário: Cianobactérias/ultraestrutura
Microscopia/métodos
Tilacoides/ultraestrutura
[Mh] Termos MeSH secundário: Absorção de Radiação
Anabaena variabilis/metabolismo
Anabaena variabilis/efeitos da radiação
Anabaena variabilis/ultraestrutura
Cianobactérias/metabolismo
Cianobactérias/efeitos da radiação
Membranas Intracelulares/ultraestrutura
Luz
Microscopia de Fluorescência
Fixação de Nitrogênio
Complexo de Proteína do Fotossistema I/metabolismo
Complexo de Proteína do Fotossistema I/efeitos da radiação
Ficobilissomas/efeitos da radiação
Ficobilissomas/ultraestrutura
Ficocianina/análise
Especificidade da Espécie
Análise Espectral/métodos
Tilacoides/metabolismo
Tilacoides/efeitos da radiação
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Photosystem I Protein Complex); 0 (Phycobilisomes); 11016-15-2 (Phycocyanin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170604
[St] Status:MEDLINE


  8 / 1086 MEDLINE  
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[PMID]:28542288
[Au] Autor:Dagnino-Leone J; Figueroa M; Mella C; Vorphal MA; Kerff F; Vásquez AJ; Bunster M; Martínez-Oyanedel J
[Ad] Endereço:Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile.
[Ti] Título:Structural models of the different trimers present in the core of phycobilisomes from Gracilaria chilensis based on crystal structures and sequences.
[So] Source:PLoS One;12(5):e0177540, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Phycobilisomes (PBS) are accessory light harvesting protein complexes that directionally transfer energy towards photosystems. Phycobilisomes are organized in a central core and rods radiating from it. Components of phycobilisomes in Gracilaria chilensis (Gch) are Phycobiliproteins (PBPs), Phycoerythrin (PE), and Phycocyanin (PC) in the rods, while Allophycocyanin (APC) is found in the core, and linker proteins (L). The function of such complexes depends on the structure of each component and their interaction. The core of PBS from cyanobacteria is mainly composed by cylinders of trimers of α and ß subunits forming heterodimers of Allophycocyanin, and other components of the core including subunits αII and ß18. As for the linkers, Linker core (LC) and Linker core membrane (LCM) are essential for the final emission towards photoreaction centers. Since we have previously focused our studies on the rods of the PBS, in the present article we investigated the components of the core in the phycobilisome from the eukaryotic algae, Gracilaria chilensis and their organization into trimers. Transmission electron microscopy provided the information for a three cylinders core, while the three dimensional structure of Allophycocyanin purified from Gch was determined by X-ray diffraction method and the biological unit was determined as a trimer by size exclusion chromatography. The protein sequences of all the components of the core were obtained by sequencing the corresponding genes and their expression confirmed by transcriptomic analysis. These subunits have seldom been reported in red algae, but not in Gracilaria chilensis. The subunits not present in the crystallographic structure were modeled to build the different composition of trimers. This article proposes structural models for the different types of trimers present in the core of phycobilisomes of Gch as a first step towards the final model for energy transfer in this system.
[Mh] Termos MeSH primário: Gracilaria/citologia
Ficobilissomas/química
Multimerização Proteica
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Sequência Conservada
Cristalografia por Raios X
Gracilaria/genética
Gracilaria/metabolismo
Ficobilissomas/metabolismo
Ficocianina/química
Ficocianina/genética
Estrutura Quaternária de Proteína
Subunidades Proteicas/química
Subunidades Proteicas/genética
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phycobilisomes); 0 (Protein Subunits); 0 (allophycocyanin); 11016-15-2 (Phycocyanin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177540


  9 / 1086 MEDLINE  
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[PMID]:28510423
[Au] Autor:Liu C; Fu Y; Li CE; Chen T; Li X
[Ad] Endereço:Department of Chemistry, Jinan University , Guangzhou 510632, China.
[Ti] Título:Phycocyanin-Functionalized Selenium Nanoparticles Reverse Palmitic Acid-Induced Pancreatic ß Cell Apoptosis by Enhancing Cellular Uptake and Blocking Reactive Oxygen Species (ROS)-Mediated Mitochondria Dysfunction.
[So] Source:J Agric Food Chem;65(22):4405-4413, 2017 Jun 07.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Accumulation of palmitic acid (PA) in human bodies could cause damage to pancreatic ß cells and lead to chronic diseases by generation of reactive oxygen species (ROS). Therefore, it is of great significance to search for nutrition-available agents with antioxidant activity to protect pancreatic islet cells against PA-induced damage. Phycocyanin (PC) and selenium (Se) have been reported to have excellent antioxidant activity. In this study, PC-functionalized selenium nanoparticles (PC-SeNPs) were synthesized to investigate the in vitro protective effects on INS-1E rat insulinoma ß cells against PA-induced cell death. A potent protective effect was achieved by regulation of particle size and PC content. Among three PC-SeNPs (165, 235, and 371 nm), PC-SeNPs-235 nm showed the highest cellular uptake and the best protective activities. For cell cycle analysis, PC-SeNPs showed a better protective effect on PA-induced INS-1E cell apoptosis than PC or SeNPs, and PC-SeNPs-235 nm exhibited the best effect. Further mechanistic studies demonstrated that PA induced overproduction of intracellular ROS, mitochondria fragmentation, activation of caspase-3, -8, and -9, and cleavage of PARP. However, pretreatment of the cells with PC-SeNPs effectively blocked these intracellular events, which suggests that PC-SeNPs could protect INS-1E cells against PA-induced cell apoptosis via attenuating oxidative stress and downstream signaling pathways. This finding provides a great promising nutritional approach for protection against diseases related to islet damage.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Células Secretoras de Insulina/efeitos dos fármacos
Mitocôndrias/efeitos dos fármacos
Ácido Palmítico/toxicidade
Ficocianina/farmacologia
Substâncias Protetoras/farmacologia
Espécies Reativas de Oxigênio/metabolismo
Selênio/farmacologia
[Mh] Termos MeSH secundário: Animais
Antioxidantes/química
Antioxidantes/farmacologia
Transporte Biológico/efeitos dos fármacos
Linhagem Celular Tumoral
Células Secretoras de Insulina/citologia
Células Secretoras de Insulina/metabolismo
Mitocôndrias/metabolismo
Nanopartículas/química
Estresse Oxidativo/efeitos dos fármacos
Ácido Palmítico/metabolismo
Ficocianina/química
Substâncias Protetoras/química
Ratos
Selênio/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Protective Agents); 0 (Reactive Oxygen Species); 11016-15-2 (Phycocyanin); 2V16EO95H1 (Palmitic Acid); H6241UJ22B (Selenium)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170619
[Lr] Data última revisão:
170619
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170517
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b00896


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[PMID]:28357609
[Au] Autor:Kunte M; Desai K
[Ad] Endereço:Department of Biological Sciences, SD School of Science, SVKM's NMIMS (Deemed to be) University, Vile Parle (W), Mumbai, 400056, India.
[Ti] Título:The Inhibitory Effect of C-phycocyanin Containing Protein Extract (C-PC Extract) on Human Matrix Metalloproteinases (MMP-2 and MMP-9) in Hepatocellular Cancer Cell Line (HepG2).
[So] Source:Protein J;36(3):186-195, 2017 Jun.
[Is] ISSN:1875-8355
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Spirulina platensis :have been studied for several biological activities. In the current study C-phycocyanin containing protein extract (C-PC extract) of Spirulina platensis have been studied for its effect on human matrix metalloproteinases (MMP-1, MMP-2 and MMP-9) and tissue inhibitors of MMPs (TIMP-1 and TIMP-2). In the present study, breast cancer cell line (MDA-MB 231) and hepatocellular cancer cell line (HepG2) were examined for inhibition of MMPs at different levels of expression after C-PC extract treatment. Herein, we have demonstrated that C-PC extract significantly reduced activity of MMP-2 by 55.13% and MMP-9 by 57.9% in HepG2 cells at 15 µg concentration. Additionally, the treatment has reduced mRNA expression of MMP-2 and MMP-9 at 20 µg concentration by 1.65-folds and 1.66-folds respectively. The C-PC extract treatment have also downregulated a mRNA expression of TIMP-2 by 1.12 folds at 20 µg concentration in HepG2 cells. Together, these results indicate that C-PC, extract successfully inhibited MMP-2 and -9 at different levels of expression and TIMP-2 at a mRNA expression level; however, extract did not have any effect on MMP-1 expressed in MDA-MB231 and TIMP-1 expressed in HepG2 cells as well as the exact mechanism of inhibition of MMP-2, MMP-9 and TIMP-2 remained unclear.
[Mh] Termos MeSH primário: Carcinoma Hepatocelular/enzimologia
Neoplasias Hepáticas/enzimologia
Metaloproteinase 2 da Matriz/metabolismo
Metaloproteinase 9 da Matriz/metabolismo
Inibidores de Metaloproteinases de Matriz/farmacologia
Proteínas de Neoplasias
Ficocianina/farmacologia
Extratos Vegetais/farmacologia
Spirulina/química
[Mh] Termos MeSH secundário: Células Hep G2
Seres Humanos
Inibidores de Metaloproteinases de Matriz/química
Proteínas de Neoplasias/antagonistas & inibidores
Proteínas de Neoplasias/metabolismo
Ficocianina/química
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Matrix Metalloproteinase Inhibitors); 0 (Neoplasm Proteins); 0 (Plant Extracts); 11016-15-2 (Phycocyanin); EC 3.4.24.24 (MMP2 protein, human); EC 3.4.24.24 (Matrix Metalloproteinase 2); EC 3.4.24.35 (MMP9 protein, human); EC 3.4.24.35 (Matrix Metalloproteinase 9)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170331
[St] Status:MEDLINE
[do] DOI:10.1007/s10930-017-9707-0



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