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[PMID]: 29446584 [Au] Autor: Sosedova LM; Novikov MA; Titov EA; Rukavishnikov VS [Ti] Título: [Induction of apoptosis in neurons of white rats under exposure of nanobiocomposite based on ag (0) nanoparticles and arabinogalactan]. [So] Source: Gig Sanit;95(12):1210-13, 2016. [Is] ISSN: 0016-9900 [Cp] País de publicação: Russia (Federation) [La] Idioma: rus [Ab] Resumo: There are presented results of the immunohistochemical study of neural tissue of outbred albino rats exposed for 9 days to the influence of the silver nanobiocomposite consisted of silver nanoparticles encapsulated into a matrix of a natural polymer - arabinogalactan. The research of albino rats was performed in 2 stages: half of the rats in each groups were decapitated immediately after the exposure (early period) and the rest animals - 6 months after the end of exposure (remote period). The impact of the studied substance was proved to cause functional changes in cells of the nervous tissue. After the subacute administration of the nanobiocomposite - argentum-arabinogalactan (nano-Ag-AG) in cells of the nervous tissue of the brain of albino rats the expression of apoptotic and anti-apoptotic protein (caspase-3 and bcl-2) was established to be changed. The number of normal neurons producing protein caspase-3 sharply increases. Herewith the number of immunonegative neurons fairly declines. Along with this there is noted the high level of bcl-2 content, one function ofwhich is the preclusion ofapoptosis. In preparations there is revealed a significant gain in the number of bcl-2 expressing neurons, however, the protective effect of the protein is not fully realized, that leads to the significantly increase in the content of damaged hyperchromatic cells. The evaluation of results of the immunohistochemical study of the nervous tissue of albino rats according to data concerning the proteins caspase-3 and bcl-2 expression permits to make a conclusion about the capability of nanoargentum encapsulated into polymer matrix by passing the blood-brain barrier to induce the triggering apoptosis cascade in neurons of the cerebral cortex. [Mh] Termos MeSH primário: Encéfalo Galactanos/farmacologia Larix Nanopartículas Metálicas/efeitos adversos Prata [Mh] Termos MeSH secundário: Animais Apoptose/efeitos dos fármacos Biopolímeros/farmacologia Barreira Hematoencefálica/metabolismo Encéfalo/efeitos dos fármacos Encéfalo/patologia Encéfalo/fisiopatologia Caspase 3/metabolismo Imuno-Histoquímica Modelos Animais Preparações de Plantas/farmacologia Proteínas Proto-Oncogênicas c-bcl-2/metabolismo Ratos Prata/efeitos adversos Prata/farmacologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biopolymers); 0 (Galactans); 0 (Plant Preparations); 0 (Proto-Oncogene Proteins c-bcl-2); 3M4G523W1G (Silver); EC 3.4.22.- (Caspase 3); SL4SX1O487 (arabinogalactan) [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180309 [Lr] Data última revisão: 180309 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180216 [St] Status: MEDLINE

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[PMID]: 29323115 [Au] Autor: Becker S; Schneider C; Okamura H; Crisp A; Amatov T; Dejmek M; Carell T [Ad] Endereço: Center for Integrated Protein Science Munich CiPSM at the Department of Chemistry, Ludwig-Maximilians-Universität München, 81377, Munich, Germany. [Ti] Título: Wet-dry cycles enable the parallel origin of canonical and non-canonical nucleosides by continuous synthesis. [So] Source: Nat Commun;9(1):163, 2018 01 11. [Is] ISSN: 2041-1723 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The molecules of life were created by a continuous physicochemical process on an early Earth. In this hadean environment, chemical transformations were driven by fluctuations of the naturally given physical parameters established for example by wet-dry cycles. These conditions might have allowed for the formation of (self)-replicating RNA as the fundamental biopolymer during chemical evolution. The question of how a complex multistep chemical synthesis of RNA building blocks was possible in such an environment remains unanswered. Here we report that geothermal fields could provide the right setup for establishing wet-dry cycles that allow for the synthesis of RNA nucleosides by continuous synthesis. Our model provides both the canonical and many ubiquitous non-canonical purine nucleosides in parallel by simple changes of physical parameters such as temperature, pH and concentration. The data show that modified nucleosides were potentially formed as competitor molecules. They could in this sense be considered as molecular fossils. [Mh] Termos MeSH primário: Biopolímeros/química Nucleosídeos/química RNA/química Água/química [Mh] Termos MeSH secundário: Terra (Planeta) Evolução Química Modelos Químicos Estrutura Molecular Origem da Vida [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Biopolymers); 0 (Nucleosides); 059QF0KO0R (Water); 63231-63-0 (RNA) [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180305 [Lr] Data última revisão: 180305 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180112 [St] Status: MEDLINE [do] DOI: 10.1038/s41467-017-02639-1

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[PMID]: 28458031 [Au] Autor: Yin L; Yuvienco C; Montclare JK [Ad] Endereço: Department of Chemical and Biomolecular Engineering, NYU Tandon School of Engineering, Brooklyn, NY 11201, United States. [Ti] Título: Protein based therapeutic delivery agents: Contemporary developments and challenges. [So] Source: Biomaterials;134:91-116, 2017 Jul. [Is] ISSN: 1878-5905 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: As unique biopolymers, proteins can be employed for therapeutic delivery. They bear important features such as bioavailability, biocompatibility, and biodegradability with low toxicity serving as a platform for delivery of various small molecule therapeutics, gene therapies, protein biologics and cells. Depending on size and characteristic of the therapeutic, a variety of natural and engineered proteins or peptides have been developed. This, coupled to recent advances in synthetic and chemical biology, has led to the creation of tailor-made protein materials for delivery. This review highlights strategies employing proteins to facilitate the delivery of therapeutic matter, addressing the challenges for small molecule, gene, protein and cell transport. [Mh] Termos MeSH primário: Materiais Biocompatíveis/química Biopolímeros/química Proteínas/química [Mh] Termos MeSH secundário: Sistemas de Liberação de Medicamentos/métodos Terapia Genética/métodos Nanotecnologia/métodos Engenharia de Proteínas/métodos [Pt] Tipo de publicação: JOURNAL ARTICLE; REVIEW [Nm] Nome de substância: 0 (Biocompatible Materials); 0 (Biopolymers); 0 (Proteins) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180228 [Lr] Data última revisão: 180228 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170502 [St] Status: MEDLINE

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[PMID]: 28471655 [Au] Autor: Milton J; Zhang T; Bellamy C; Swayze E; Hart C; Weisser M; Hecht S; Rotstein S [Ad] Endereço: Ionis Pharmaceuticals, Inc , 2855 Gazelle Court, Carlsbad, California 92010, United States. [Ti] Título: HELM Software for Biopolymers. [So] Source: J Chem Inf Model;57(6):1233-1239, 2017 06 26. [Is] ISSN: 1549-960X [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Hierarchical Editing Language for Macromolecules (HELM version 2.0) is a molecular line notation similar to SMILEs but specifically for communicating and managing biopolymer structures. The HELM project, part of the Pistoia Alliance nonprofit organization, has been tasked to develop and promote HELM as a global exchange format and recently released version 2.0 of the specification. Here we will describe the specifics of the HELM v2.0 notation along with the large ecosystem of software to support HELM-based structure management. We will highlight a recent open-source software and database for HELM monomers and a new, simpler approach to deploying a large complicated molecular management system. [Mh] Termos MeSH primário: Biopolímeros/química Biologia Computacional/métodos Software [Mh] Termos MeSH secundário: Bases de Dados Factuais Internet [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biopolymers) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 180227 [Lr] Data última revisão: 180227 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170505 [St] Status: MEDLINE [do] DOI: 10.1021/acs.jcim.6b00442

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[PMID]: 29241956 [Au] Autor: Abd Wahib SM; Wan Ibrahim WA; Sanagi MM; Kamboh MA; Abdul Keyon AS [Ad] Endereço: Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia. [Ti] Título: Magnetic sporopollenin-cyanopropyltriethoxysilane-dispersive micro-solid phase extraction coupled with high performance liquid chromatography for the determination of selected non-steroidal anti-inflammatory drugs in water samples. [So] Source: J Chromatogr A;1532:50-57, 2018 Jan 12. [Is] ISSN: 1873-3778 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: A facile dispersive-micro-solid phase extraction (D-µ-SPE) method coupled with HPLC for the analysis of selected non-steroidal anti-inflammatory drugs (NSAIDs) in water samples was developed using a newly prepared magnetic sporopollenin-cyanopropyltriethoxysilane (MS-CNPrTEOS) sorbent. Sporopollenin homogenous microparticles of Lycopodium clavatum spores possessed accessible functional groups that facilitated surface modification. Simple modification was performed by functionalization with 3-cyanopropyltriethoxysilane (CNPrTEOS) and magnetite was introduced onto the biopolymer to simplify the extraction process. MS-CNPrTEOS was identified by infrared spectrometrywhile the morphology and the magnetic property were confirmed by scanning electron microscopy (SEM) and vibrating sample magnetometer (VSM), respectively. To maximize the extraction performance of ketoprofen, ibuprofen, diclofenac and mefenamic acid using the proposed MS-CNPrTEOS, important D-µ-SPE parameters were comprehensively optimized. The optimum extraction conditions were sorbent amount, 40 mg; extraction time, 5 min; desorption time; 5 min; sample volume, 15 mL; sample pH 2.0; and salt addition, 2.5% (w/v). The feasibility of the developed method was evaluated using spiked tap water, lake water, river water and waste water samples. Results showed that ketoprofen and ibuprofen were linear in the range of 1.0-1000 µg L whilst diclofenac and mefenamic acid were linear in the range 0.8-500 µg L . The results also showed good detection limits for the studied NSAIDs in the range of 0.21-0.51 µg L and good recoveries for spiked water samples in the range of 85.1-106.4%. The MS-CNPrTEOS proved a promising dispersive sorbent and applicable to facile and rapid assay of NSAIDs in water samples. [Mh] Termos MeSH primário: Anti-Inflamatórios não Esteroides/análise Cromatografia Líquida de Alta Pressão Monitoramento Ambiental/métodos Água Doce/química Microextração em Fase Sólida [Mh] Termos MeSH secundário: Biopolímeros/análise Biopolímeros/química Carotenoides/química Ibuprofeno/análise Cetoprofeno/análise Limite de Detecção Magnetismo Águas Residuais/química Poluentes Químicos da Água/análise [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Biopolymers); 0 (Waste Water); 0 (Water Pollutants, Chemical); 12712-72-0 (sporopollenin); 36-88-4 (Carotenoids); 90Y4QC304K (Ketoprofen); WK2XYI10QM (Ibuprofen) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180222 [Lr] Data última revisão: 180222 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171216 [St] Status: MEDLINE

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[PMID]: 29037702 [Au] Autor: Benbettaïeb N; Tanner C; Cayot P; Karbowiak T; Debeaufort F [Ad] Endereço: Univ. Bourgogne Franche-Comté/Agrosup Dijon, UMR PAM A02-102, Food and Wine Physical-Chemistry Lab, 1 esplanade Erasme, 21000 Dijon, France; IUT-Dijon-Auxerre, Dpt BioEngineering, 7 blvd Docteur Petitjean, 20178 Dijon Cedex, France. [Ti] Título: Impact of functional properties and release kinetics on antioxidant activity of biopolymer active films and coatings. [So] Source: Food Chem;242:369-377, 2018 Mar 01. [Is] ISSN: 0308-8146 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: This work deals with the study of the release kinetics of some natural antioxidants (ferulic acid, caffeic acid and tyrosol) from chitosan-fish gelatin edible films immersed ethanol at 96%, as well as the kinetics of their antioxidant activity using the DPPH assay. The aim was to determine how film functional properties influence the release kinetic and antioxidant activity. The addition of antioxidants to chitosan-fish gelatin matrix decreased the water vapour permeability by more than 30%. The tensile strength (TS) increased up to 50% after the incorporation of antioxidants. Some molecular interactions between polymer chains and antioxidants were confirmed by FTIR where spectra displayed a shift of the amide-III peak. Films containing caffeic acid or a caffeic-ferulic acid mixture exhibited the highest radical scavenging activity, leading to a 90% antioxidant activity at equilibrium but the release rate controlled the efficacy of the system. [Mh] Termos MeSH primário: Antioxidantes/análise Biopolímeros/química Quitosana/química Gelatina/química [Mh] Termos MeSH secundário: Antioxidantes/química Ácidos Cafeicos/análise Ácidos Cafeicos/química Ácidos Cumáricos/análise Ácidos Cumáricos/química Produtos Pesqueiros Cinética Permeabilidade Álcool Feniletílico/análogos & derivados Álcool Feniletílico/análise Álcool Feniletílico/química Resistência à Tração [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antioxidants); 0 (Biopolymers); 0 (Caffeic Acids); 0 (Coumaric Acids); 1AK4MU3SNX (4-hydroxyphenylethanol); 9000-70-8 (Gelatin); 9012-76-4 (Chitosan); AVM951ZWST (ferulic acid); ML9LGA7468 (Phenylethyl Alcohol); U2S3A33KVM (caffeic acid) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180222 [Lr] Data última revisão: 180222 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171018 [St] Status: MEDLINE

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[PMID]: 28464938 [Au] Autor: Monge C; DiStasio N; Rossi T; Sébastien M; Sakai H; Kalman B; Boudou T; Tajbakhsh S; Marty I; Bigot A; Mouly V; Picart C [Ad] Endereço: CNRS, UMR 5628, LMGP, 3 parvis Louis Néel, F-38016, Grenoble, France. claire.monge@ibcp.fr. [Ti] Título: Quiescence of human muscle stem cells is favored by culture on natural biopolymeric films. [So] Source: Stem Cell Res Ther;8(1):104, 2017 May 02. [Is] ISSN: 1757-6512 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Satellite cells are quiescent resident muscle stem cells that present an important potential to regenerate damaged tissue. However, this potential is diminished once they are removed from their niche environment in vivo, prohibiting the long-term study and genetic investigation of these cells. This study therefore aimed to provide a novel biomaterial platform for the in-vitro culture of human satellite cells that maintains their stem-like quiescent state, an important step for cell therapeutic studies. METHODS: Human muscle satellite cells were isolated from two donors and cultured on soft biopolymeric films of controlled stiffness. Cell adhesive phenotype, maintenance of satellite cell quiescence and capacity for gene manipulation were investigated using FACS, western blotting, fluorescence microscopy and electron microscopy. RESULTS: About 85% of satellite cells cultured in vitro on soft biopolymer films for 3 days maintained expression of the quiescence marker Pax7, as compared with 60% on stiffer films and 50% on tissue culture plastic. The soft biopolymeric films allowed satellite cell culture for up to 6 days without renewing the media. These cells retained their stem-like properties, as evidenced by the expression of stem cell markers and reduced expression of differentiated markers. In addition, 95% of cells grown on these soft biopolymeric films were in the G0/G1 stage of the cell cycle, as opposed to those grown on plastic that became activated and began to proliferate and differentiate. CONCLUSIONS: Our study identifies a new biomaterial made of a biopolymer thin film for the maintenance of the quiescence state of muscle satellite cells. These cells could be activated at any point simply by replating them onto a plastic culture dish. Furthermore, these cells could be genetically manipulated by viral transduction, showing that this biomaterial may be further used for therapeutic strategies. [Mh] Termos MeSH primário: Células-Tronco Adultas/citologia Proliferação Celular Cultura Primária de Células/métodos Células Satélites de Músculo Esquelético/citologia [Mh] Termos MeSH secundário: Células-Tronco Adultas/efeitos dos fármacos Células-Tronco Adultas/fisiologia Biopolímeros/farmacologia Diferenciação Celular Células Cultivadas Meios de Cultura/química Seres Humanos Masculino Meia-Idade Células Satélites de Músculo Esquelético/efeitos dos fármacos Células Satélites de Músculo Esquelético/fisiologia Tecidos Suporte/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biopolymers); 0 (Culture Media) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180222 [Lr] Data última revisão: 180222 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170504 [St] Status: MEDLINE [do] DOI: 10.1186/s13287-017-0556-8

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[PMID]: 28693863 [Au] Autor: Nowacka M; Fijalkowska A; Dadan M; Rybak K; Wiktor A; Witrowa-Rajchert D [Ad] Endereço: Department of Food Engineering and Process Management, Faculty of Food Sciences, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159c, 02-776 Warsaw, Poland. Electronic address: malgorzata_nowacka@sggw.pl. [Ti] Título: Effect of ultrasound treatment during osmotic dehydration on bioactive compounds of cranberries. [So] Source: Ultrasonics;83:18-25, 2018 Feb. [Is] ISSN: 1874-9968 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: The aim of the study was to investigate the influence of ultrasound treatment applied in osmotic solution on bioactive compounds, such as vitamin C, polyphenols, anthocyanins and flavonoids content as well as antioxidant activity in cranberries (Vaccinium oxycoccus). Ultrasound treatment was performed at the frequency of 21kHz for 30 and 60min in two osmotic solutions - 61.5% sucrose and 30% sucrose with an addition of 0.1% of steviol glycosides. Before the ultrasound treatment the material was subjected to cutting or blanching. The obtained results indicated that the influence of ultrasound waves on cranberries depends on a type of bioactive component. The ultrasound treated cranberries as well as the ones subjected to cutting or blanching enhanced by ultrasound were characterized mainly by a lower content of bioactive compounds. [Mh] Termos MeSH primário: Biopolímeros/química Biopolímeros/efeitos da radiação Dessecação/métodos Osmose/efeitos da radiação Ondas Ultrassônicas Vaccinium macrocarpon/química Vaccinium macrocarpon/efeitos da radiação [Mh] Termos MeSH secundário: Relação Dose-Resposta à Radiação Conservação de Alimentos/métodos Dose de Radiação [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biopolymers) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180212 [Lr] Data última revisão: 180212 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170712 [St] Status: MEDLINE

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[PMID]: 29257871 [Au] Autor: Wang H; Qian J; Ding F [Ad] Endereço: School of Printing and Packaging, Wuhan University , Wuhan 430072, PR China. [Ti] Título: Emerging Chitosan-Based Films for Food Packaging Applications. [So] Source: J Agric Food Chem;66(2):395-413, 2018 Jan 17. [Is] ISSN: 1520-5118 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Recent years have witnessed great developments in biobased polymer packaging films for the serious environmental problems caused by the petroleum-based nonbiodegradable packaging materials. Chitosan is one of the most abundant biopolymers after cellulose. Chitosan-based materials have been widely applied in various fields for their biological and physical properties of biocompatibility, biodegradability, antimicrobial ability, and easy film forming ability. Different chitosan-based films have been fabricated and applied in the field of food packaging. Most of the review papers related to chitosan-based films are focusing on antibacterial food packaging films. Along with the advances in the nanotechnology and polymer science, numerous strategies, for instance direct casting, coating, dipping, layer-by-layer assembly, and extrusion, have been employed to prepare chitosan-based films with multiple functionalities. The emerging food packaging applications of chitosan-based films as antibacterial films, barrier films, and sensing films have achieved great developments. This article comprehensively reviews recent advances in the preparation and application of engineered chitosan-based films in food packaging fields. [Mh] Termos MeSH primário: Biopolímeros/química Quitosana/química Embalagem de Alimentos/instrumentação [Mh] Termos MeSH secundário: Animais Embalagem de Alimentos/métodos Embalagem de Alimentos/tendências [Pt] Tipo de publicação: JOURNAL ARTICLE; REVIEW [Nm] Nome de substância: 0 (Biopolymers); 9012-76-4 (Chitosan) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180205 [Lr] Data última revisão: 180205 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171220 [St] Status: MEDLINE [do] DOI: 10.1021/acs.jafc.7b04528

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[PMID]: 29240759 [Au] Autor: Zanotti G; Vallese F; Ferrari A; Menozzi I; Saldaño TE; Berto P; Fernandez-Alberti S; Berni R [Ad] Endereço: Department of Biomedical Sciences, University of Padua, Padua, Italy. [Ti] Título: Structural and dynamics evidence for scaffold asymmetric flexibility of the human transthyretin tetramer. [So] Source: PLoS One;12(12):e0187716, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The molecular symmetry of multimeric proteins is generally determined by using X-ray diffraction techniques, so that the basic question as to whether this symmetry is perfectly preserved for the same protein in solution remains open. In this work, human transthyretin (TTR), a homotetrameric plasma transport protein with two binding sites for the thyroid hormone thyroxine (T4), is considered as a case study. Based on the crystal structure of the TTR tetramer, a hypothetical D2 symmetry is inferred for the protein in solution, whose functional behavior reveals the presence of two markedly different Kd values for the two T4 binding sites. The latter property has been ascribed to an as yet uncharacterized negative binding cooperativity. A triple mutant form of human TTR (F87M/L110M/S117E TTR), which is monomeric in solution, crystallizes as a tetrameric protein and its structure has been determined. The exam of this and several other crystal forms of human TTR suggests that the TTR scaffold possesses a significant structural flexibility. In addition, TTR tetramer dynamics simulated using normal modes analysis exposes asymmetric vibrational patterns on both dimers and thermal fluctuations reveal small differences in size and flexibility for ligand cavities at each dimer-dimer interface. Such small structural differences between monomers can lead to significant functional differences on the TTR tetramer dynamics, a feature that may explain the functional heterogeneity of the T4 binding sites, which is partially overshadowed by the crystal state. [Mh] Termos MeSH primário: Biopolímeros/química Pré-Albumina/química [Mh] Termos MeSH secundário: Cristalografia por Raios X Seres Humanos Conformação Proteica Proteínas Recombinantes/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biopolymers); 0 (Prealbumin); 0 (Recombinant Proteins) [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180116 [Lr] Data última revisão: 180116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171215 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0187716

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