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[PMID]:27771811
[Au] Autor:Kamenskiy A; Seas A; Deegan P; Poulson W; Anttila E; Sim S; Desyatova A; MacTaggart J
[Ad] Endereço:Department of Surgery, 987690 Nebraska Medical Center, University of Nebraska Medical Center, Omaha, NE, 68198-7690, USA. Alexey.Kamenskiy@unmc.edu.
[Ti] Título:Constitutive description of human femoropopliteal artery aging.
[So] Source:Biomech Model Mechanobiol;16(2):681-692, 2017 04.
[Is] ISSN:1617-7940
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Femoropopliteal artery (FPA) mechanics play a paramount role in pathophysiology and the artery's response to therapeutic interventions, but data on FPA mechanical properties are scarce. Our goal was to characterize human FPAs over a wide population to derive a constitutive description of FPA aging to be used for computational modeling. Fresh human FPA specimens ([Formula: see text]) were obtained from [Formula: see text] predominantly male (80 %) donors 54±15 years old (range 13-82 years). Morphometric characteristics including radius, wall thickness, opening angle, and longitudinal pre-stretch were recorded. Arteries were subjected to multi-ratio planar biaxial extension to determine constitutive parameters for an invariant-based model accounting for the passive contributions of ground substance, elastin, collagen, and smooth muscle. Nonparametric bootstrapping was used to determine unique sets of material parameters that were used to derive age-group-specific characteristics. Physiologic stress-stretch state was calculated to capture changes with aging. Morphometric and constitutive parameters were derived for seven age groups. Vessel radius, wall thickness, and circumferential opening angle increased with aging, while longitudinal pre-stretch decreased ([Formula: see text]). Age-group-specific constitutive parameters portrayed orthotropic FPA stiffening, especially in the longitudinal direction. Structural changes in artery wall elastin were associated with reduction of physiologic longitudinal and circumferential stretches and stresses with age. These data and the constitutive description of FPA aging shed new light on our understanding of peripheral arterial disease pathophysiology and arterial aging. Application of this knowledge might improve patient selection for specific treatment modalities in personalized, precision medicine algorithms and could assist in device development for treatment of peripheral artery disease.
[Mh] Termos MeSH primário: Envelhecimento
Artérias/fisiologia
Modelos Biológicos
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Fenômenos Biomecânicos
Colágeno/metabolismo
Elastina/metabolismo
Feminino
Seres Humanos
Masculino
Meia-Idade
Doença Arterial Periférica/fisiopatologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
9007-34-5 (Collagen); 9007-58-3 (Elastin)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM; S
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1007/s10237-016-0845-7


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[PMID]:29291445
[Au] Autor:Bouhedja M; Peres B; Fhayli W; Ghandour Z; Boumendjel A; Faury G; Khelili S
[Ad] Endereço:Laboratoire de Phytochimie et de Pharmacologie, Département de Chimie, Faculté des Sciences Exactes et Informatique, Université Mohamed Seddik Ben Yahia Jijel, B.P. 98 Ouled Aissa, 18000 Jijel, Algeria; Université Grenoble-Alpes/CNRS, Département de Pharmacochimie Moléculaire UMR 5063, F-38041 Greno
[Ti] Título:Design, synthesis and biological evaluation of novel ring-opened cromakalim analogues with relaxant effects on vascular and respiratory smooth muscles and as stimulators of elastin synthesis.
[So] Source:Eur J Med Chem;144:774-796, 2018 Jan 20.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Two new series of ring-opened analogues of cromakalim bearing sulfonylurea moieties (series A: with N-unmethylated sulfonylureas, series B: with N-methylated sulfonylureas) were synthesized and tested as relaxants of vascular and respiratory smooth muscles (rat aorta and trachea, respectively). Ex vivo biological evaluations indicated that the most active compounds, belonging to series B, displayed a marked vasorelaxant activity on endothelium-intact aortic rings and the trachea. A majority of series B compounds exhibited a higher vasorelaxant activity (EC < 22 µM) than that of the reference compound diazoxide (EC = 24 µM). Interestingly, several tested compounds of series B also presented stronger relaxant effects on the trachea than the reference compound cromakalim (EC = 124 µM), in particular compounds B4, B7 and B16 (EC < 10 µM). By contrast, series A derivatives were poorly active on aortic rings (EC > 57 µM for all, and EC > 200 µM for a majority of them), but some of them showed an interesting relaxing effect on trachea (i.e. A15 and A33, EC = 30 µM). The most potent compounds of both series, i.e. A15, A33 and B16, tested on aortic rings in the presence of glibenclamide or 80 mM KCl, suggested that they acted as voltage-gated Ca channel blockers, like verapamil, instead of being ATP-potassium channel activators, as is cromakalim, the parent molecule. Further investigations on cultured vascular smooth muscle cells showed a strong stimulating effect on elastin synthesis, especially compound B16, which was more active at 20 µM than diazoxide, a reference ATP-sensitive potassium channel activator. Taken together, our results show that the N-methylation of the sulfonylurea moieties of ring-opened cromakalim analogues led to new compounds blocking calcium-gated channels, which had a major impact on the arterial and tracheal activities as well as selectivity.
[Mh] Termos MeSH primário: Cromakalim/farmacologia
Desenho de Drogas
Elastina/biossíntese
Músculo Liso/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Cromakalim/síntese química
Cromakalim/química
Relação Dose-Resposta a Droga
Feminino
Estrutura Molecular
Contração Muscular/efeitos dos fármacos
Ratos
Ratos Wistar
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0G4X367WA3 (Cromakalim); 9007-58-3 (Elastin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180102
[St] Status:MEDLINE


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[PMID]:29242152
[Au] Autor:Hou C; Peng D; Gao L; Tian D; Dai J; Luo Z; Liu E; Chen H; Zou L; Fu Z
[Ad] Endereço:Pediatrics Research Institute, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing 400014, China; Chongqing Key Laboratory of Pediatrics, China; China International Science and Technology Cooperation Base of Child De
[Ti] Título:Human umbilical cord-derived mesenchymal stem cells protect from hyperoxic lung injury by ameliorating aberrant elastin remodeling in the lung of O -exposed newborn rat.
[So] Source:Biochem Biophys Res Commun;495(2):1972-1979, 2018 01 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The incidence and mortality rates of bronchopulmonary dysplasia (BPD) remain very high. Therefore, novel therapies are imminently needed to improve the outcome of this disease. Human umbilical cord-derived mesenchymal stem cells (UC-MSCs) show promising therapeutic effects on oxygen-induced model of BPD. In our experiment, UC-MSCs were intratracheally delivered into the newborn rats exposed to hyperoxia, a well-established BPD model. This study demonstrated that UC-MSCs reduce elastin expression stimulated by 90% O in human lung fibroblasts-a (HLF-a), and inhibit HLF-a transdifferentiation into myofibroblasts. In addition, the therapeutic effects of UC-MSCs in neonatal rats with BPD, UC-MSCs could inhibit lung elastase activity and reduce aberrant elastin expression and deposition in the lung of BPD rats. Overall, this study suggested that UC-MSCs could ameliorate aberrant elastin expression in the lung of hyperoxia-induced BPD model which may be associated with suppressing increased TGFß1 activation.
[Mh] Termos MeSH primário: Displasia Broncopulmonar/imunologia
Displasia Broncopulmonar/patologia
Transplante de Células-Tronco de Sangue do Cordão Umbilical/métodos
Elastina/metabolismo
Pulmão/imunologia
Pulmão/patologia
Transplante de Células-Tronco Mesenquimais/métodos
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Células Cultivadas
Seres Humanos
Oxigenação Hiperbárica
Hiperóxia/metabolismo
Hiperóxia/patologia
Hiperóxia/prevenção & controle
Lesão Pulmonar/imunologia
Lesão Pulmonar/patologia
Lesão Pulmonar/prevenção & controle
Ratos
Ratos Sprague-Dawley
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
9007-58-3 (Elastin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE


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[PMID]:28455451
[Au] Autor:Okamura H; Emrich F; Trojan J; Chiu P; Dalal AR; Arakawa M; Sato T; Penov K; Koyano T; Pedroza A; Connolly AJ; Rabinovitch M; Alvira C; Fischbein MP
[Ad] Endereço:Department of Cardiothoracic Surgery, Stanford University, Stanford, California.
[Ti] Título:Long-term miR-29b suppression reduces aneurysm formation in a Marfan mouse model.
[So] Source:Physiol Rep;5(8), 2017 Apr.
[Is] ISSN:2051-817X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Aortic root aneurysm formation and subsequent dissection and/or rupture remain the leading cause of death in patients with Marfan syndrome. Our laboratory has reported that miR-29b participates in aortic root/ascending aorta extracellular matrix remodeling during early aneurysm formation in Marfan mice. Herein, we sought to determine whether miR-29b suppression can reduce aneurysm formation long-term. Marfan mice were treated with retro-orbital LNA-anti-miR-29b inhibitor or scrambled-control-miR before aneurysms develop either (1) a single dose prenatally (pregnant mice at 14.5 days post-coitum) ( = 8-10, each group) or (2) postnatally every other week, from 2 to 22 weeks of age, and sacrificed at 24 weeks ( = 8-10, each group). To determine if miR-29b blockade was beneficial even after aneurysms develop, a third group of animals were treated every other week, starting at 8 weeks of age, until sacrificed ( = 4-6, each group). miR-29b inhibition resulted in aneurysm reduction, increased elastogenesis, decreased matrix metalloproteinase activity and decreased elastin breakdown. Prenatal LNA-anti-miR-29b inhibitor treatment decreased aneurysm formation up to age 32 weeks, whereas postnatal treatment was effective up to 16 weeks. miR-29b blockade did not slow aortic growth once aneurysms already developed. Systemic miR-29b inhibition significantly reduces aneurysm development long-term in a Marfan mouse model. Drug administration during aortic wall embryologic development appears fundamental. miR-29b suppression could be a potential therapeutic target for reducing aneurysm formation in Marfan syndrome patients.
[Mh] Termos MeSH primário: Aneurisma Aórtico/prevenção & controle
Terapia Genética/métodos
Síndrome de Marfan/terapia
MicroRNAs/antagonistas & inibidores
[Mh] Termos MeSH secundário: Animais
Aneurisma Aórtico/diagnóstico por imagem
Aneurisma Aórtico/etiologia
Aneurisma Aórtico/patologia
Modelos Animais de Doenças
Progressão da Doença
Ecocardiografia
Elastina/metabolismo
Matriz Extracelular/fisiologia
Feminino
Terapias Fetais/métodos
Masculino
Síndrome de Marfan/complicações
Síndrome de Marfan/genética
Metaloproteinases da Matriz/fisiologia
Camundongos Endogâmicos C57BL
Camundongos Mutantes
Terapia de Alvo Molecular/métodos
Cuidado Pré-Natal/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MIRN29 microRNA, mouse); 0 (MicroRNAs); 9007-58-3 (Elastin); EC 3.4.24.- (Matrix Metalloproteinases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


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[PMID]:29225168
[Au] Autor:Chai H; Tao Z; Chen W; Xu Y; Huang F; Su C; Chen X
[Ad] Endereço:The Department of Thoracic and Cardiovascular Surgery, Nanjing First Hospital, Nanjing Medical University, Changle Road 68, Nanjing 210006, Jiangsu, People's Republic of China.
[Ti] Título:Cortistatin attenuates angiotensin II-induced abdominal aortic aneurysm through inactivation of the ERK1/2 signaling pathways.
[So] Source:Biochem Biophys Res Commun;495(2):1801-1806, 2018 01 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Abdominal aortic aneurysm (AAA) is a fatal disease that is associated with chronic inflammation in the vessel wall. Cortistatin is implicated in inflammation, vascular smooth muscle cell migration and other cardiovascular pathologies. But, the hypothetical effect of cortistatin on AAA remains uncertain. We investigated the effect of cortistatin administration to angiotensin (Ang) II-induced AAA formation in apolipoprotein E deficient (Apoe ) mice. We showed that cortistatin administration significantly suppresses incidence and severity of AAA in Apoe mice. A significant increase in macrophage infiltration, excretion of inflammatory cytokines, activities and expression levels of MMP2 and MMP9, reactive oxygen species levels and cell apoptosis in aneurysmal aortic wall of Apoe mice infused with Ang-II, and these events were significantly alleviated by co-treatment with cortistatin. Mechanistic studies showed that the protective effects of cortistatin were related to the blocking of ERK1/2 signaling pathways, while does not was not actually affect JNK, P38 phosphorylation. In conclusion, cortistatin appears to play an essential role in the formation of AAA and indicate cortistatin may as novel therapeutic option for AAA.
[Mh] Termos MeSH primário: Aneurisma da Aorta Abdominal/prevenção & controle
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Neuropeptídeos/administração & dosagem
[Mh] Termos MeSH secundário: Angiotensina II/administração & dosagem
Animais
Aorta Abdominal/efeitos dos fármacos
Aorta Abdominal/metabolismo
Aorta Abdominal/patologia
Aneurisma da Aorta Abdominal/etiologia
Aneurisma da Aorta Abdominal/metabolismo
Apoptose/efeitos dos fármacos
Linhagem Celular
Modelos Animais de Doenças
Elastina/metabolismo
Masculino
Metaloproteinase 2 da Matriz/metabolismo
Metaloproteinase 9 da Matriz/metabolismo
Camundongos
Camundongos Knockout para ApoE
Miócitos de Músculo Liso/efeitos dos fármacos
Miócitos de Músculo Liso/patologia
Proteólise/efeitos dos fármacos
Espécies Reativas de Oxigênio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Neuropeptides); 0 (Reactive Oxygen Species); 0 (cortistatin); 11128-99-7 (Angiotensin II); 9007-58-3 (Elastin); EC 3.4.24.24 (Matrix Metalloproteinase 2); EC 3.4.24.24 (Mmp2 protein, mouse); EC 3.4.24.35 (Matrix Metalloproteinase 9); EC 3.4.24.35 (Mmp9 protein, mouse)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE


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Martins, Pedro
Texto completo
[PMID]:28454911
[Au] Autor:Rynkevic R; Martins P; Hympanova L; Almeida H; Fernandes AA; Deprest J
[Ad] Endereço:University of Porto, Faculty of Engineering, Portugal; INEGI, University of Porto, Faculty of Engineering, Portugal; KU Leuven, Department Development and Regeneration, Biomedical Sciences, Leuven, Belgium; Centre for Surgical Technologies, Group Biomedical Sciences, Belgium. Electronic address: r.r
[Ti] Título:Biomechanical and morphological properties of the multiparous ovine vagina and effect of subsequent pregnancy.
[So] Source:J Biomech;57:94-102, 2017 05 24.
[Is] ISSN:1873-2380
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pelvic floor soft tissues undergo changes during the pregnancy. However, the degree and nature of this process is not completely characterized. This study investigates the effect of subsequent pregnancy on biomechanical and structural properties of ovine vagina. Vaginal wall from virgin, pregnant (in their third pregnancy) and parous (one year after third vaginal delivery) Swifter sheep (n=5 each) was harvested. Samples for biomechanics and histology, were cut in longitudinal axis (proximal and distal regions). Outcome measurements describing Young's modulus, ultimate stress and elongation were obtained from stress-strain curves. For histology samples were stained with Miller's Elastica staining. Collagen, elastin and muscle cells and myofibroblasts contents were estimated, using image processing techniques. Statistical analyses were performed in order to determine significant differences among experimental groups. Significant regional differences were identified. The proximal vagina was stiffer than distal, irrespective the reproductive status. During the pregnancy proximal vagina become more compliant than in parous (+47.45%) or virgin sheep (+64.35%). This coincided with lower collagen (-15 to -21%), higher elastin (+30 to +60%), and more smooth muscle cells (+17 to +37%). Vaginal tissue from parous ewes was weaker than of virgins, coinciding with lower collagen (-10%), higher elastin (+50%), more smooth muscle cells (+20%). It could be proposed that after pregnancy biomechanical properties of vagina do not recover to those of virgins. Since elastin has a significant influence on the compliance of soft tissues and collagen is the main "actor" regarding strength, histological analysis performed in this study justifies the mechanical behavior observed.
[Mh] Termos MeSH primário: Gravidez/fisiologia
Vagina/fisiologia
[Mh] Termos MeSH secundário: Animais
Fenômenos Biomecânicos
Colágeno/fisiologia
Complacência (Medida de Distensibilidade)
Módulo de Elasticidade
Elastina/fisiologia
Feminino
Processamento de Imagem Assistida por Computador
Miócitos de Músculo Liso/fisiologia
Paridade
Ovinos
Vagina/anatomia & histologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
9007-34-5 (Collagen); 9007-58-3 (Elastin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180120
[Lr] Data última revisão:
180120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


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[PMID]:29235321
[Au] Autor:Nidialkova NA; Varbanets LD; Chernyshenko VO
[Ti] Título:Isolation and purification of Bacillus thuringiensis var. israelensis IÐœV Ð’-7465 peptidase with specificity toward elastin and collagen.
[So] Source:Ukr Biochem J;88(3):18-28, 2016 May-Jun.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:Peptidase of Bacillus thuringiensis var. israelensis IÐœV Ð’-7465 was isolated from culture supernatant using consecutive fractionations by an ammonium sulphate (60% saturation), ion-exchange chromatography and gel-filtration on the TSK-gels Toyoperl HW-55 and DEAE 650(M). Specific elastase (442 U∙mg of protein-1) and collagenase (212.7 U∙mg of protein-1) activities of the purified enzyme preparation were 8.0- and 6.1-fold, respectively higher than ones of the culture supernatant. Peptidase yields were 33.5% for elastase activity and 30.1% for collagenase activity. It was established that the enzyme is serine metal-dependent alkaline peptidase with Mr about 37 kDa. Maximal hydrolysis of elastin and collagen occurs at the optimum pH 8.0 and t° ­ 40 and 50 °Ð¡, respectively. The purified preparation has high stability at pH in the range of 7.0 to 10.0 and 40-50 °Ð¡.
[Mh] Termos MeSH primário: Bacillus thuringiensis/química
Proteínas de Bactérias/isolamento & purificação
Colágeno/química
Elastina/química
Peptídeo Hidrolases/isolamento & purificação
[Mh] Termos MeSH secundário: Sulfato de Amônio/química
Bacillus thuringiensis/enzimologia
Proteínas de Bactérias/química
Cromatografia em Gel
Cromatografia por Troca Iônica
Ensaios Enzimáticos
Estabilidade Enzimática
Concentração de Íons de Hidrogênio
Hidrólise
Peso Molecular
Peptídeo Hidrolases/química
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 9007-34-5 (Collagen); 9007-58-3 (Elastin); EC 3.4.- (Peptide Hydrolases); SU46BAM238 (Ammonium Sulfate)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.03.018


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[PMID]:28452589
[Au] Autor:Griner JD; Rogers CJ; Zhu MJ; Du M
[Ad] Endereço:a Department of Animal Sciences , Washington State University , Pullman , WA , USA.
[Ti] Título:Lysyl oxidase propeptide promotes adipogenesis through inhibition of FGF-2 signaling.
[So] Source:Adipocyte;6(1):12-19, 2017 01 02.
[Is] ISSN:2162-397X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lysyl oxidase (LOX) catalyzes the oxidative deamination of lysine residues in collagen and elastin, key components of connective tissue. LOX is synthesized as an inactive 50 kD pre-proenzyme, and secreted to the extracellular matrix where it is cleaved into an active 32 kD LOX, and an 18kD free propeptide (LOX-PP), purportedly an inhibitor of fibroblast growth factor-2 (FGF-2) signaling. Given that adipocytes are distributed inside the connective tissue, it is likely that LOX-PP has an important regulatory role in adipogenesis, which has not been studied. Using NIH 3T3-L1 cells, we observed that FGF-2 inhibited adipogenesis, and LOX-PP promoted adipogenesis of 3T3-L1 cells in the presence of FGF-2; the expression of peroxisome proliferator-activated receptor (PPAR) γ and CCAAT-enhancer binding protein (C/EBP) α, two markers of adipogenesis, were enhanced in the presence of LOX-PP. We further observed that LOX-PP down-regulated AKT and ERK1/2, two proliferative signaling proteins down-stream of FGF-2 signaling. Similarly, inhibition of FGF-2 receptor signaling by canofin, a competitive inhibitor of FGF-2 receptor, promoted adipogenesis albeit less effective compared to LOX-PP. To further explore whether LOX-PP promoted adipogenesis through inhibition of FGF-2 signaling, site directed mutagenesis of LOX-PP, resulting in an Arg158 to Gln158 mutation which abolishes the inhibitory activity of LOX-PP to FGF-2 receptor, attenuated the adipogenic promoting properties of LOX-PP. In summary, for the first time, our data show that LOX-PP enhances adipogenesis at least partially through inhibition of FGF-2 receptor signaling. Our data suggest that LOX-PP may serve as a bona fide therapeutic target for regulating adipogenesis and adipose tissue development.
[Mh] Termos MeSH primário: Adipogenia/fisiologia
Proteína-Lisina 6-Oxidase/biossíntese
Proteína-Lisina 6-Oxidase/metabolismo
[Mh] Termos MeSH secundário: Células 3T3-L1
Adipócitos/metabolismo
Animais
Proteína alfa Estimuladora de Ligação a CCAAT/metabolismo
Proliferação Celular/fisiologia
Colágeno/metabolismo
Elastina/metabolismo
Precursores Enzimáticos/metabolismo
Fator 2 de Crescimento de Fibroblastos/antagonistas & inibidores
Fator 2 de Crescimento de Fibroblastos/metabolismo
Fator 2 de Crescimento de Fibroblastos/fisiologia
Lisina/metabolismo
Camundongos
PPAR gama/metabolismo
Proteína-Lisina 6-Oxidase/química
Transdução de Sinais/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CCAAT-Enhancer-Binding Protein-alpha); 0 (Enzyme Precursors); 0 (PPAR gamma); 103107-01-3 (Fibroblast Growth Factor 2); 9007-34-5 (Collagen); 9007-58-3 (Elastin); EC 1.4.3.13 (Protein-Lysine 6-Oxidase); K3Z4F929H6 (Lysine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1080/21623945.2016.1271511


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[PMID]:28934329
[Au] Autor:Gluck JM; Herren AW; Yechikov S; Kao HKJ; Khan A; Phinney BS; Chiamvimonvat N; Chan JW; Lieu DK
[Ad] Endereço:Division of Cardiovascular Medicine, Department of Internal Medicine, University of California Davis; Davis, CA, United States of America.
[Ti] Título:Biochemical and biomechanical properties of the pacemaking sinoatrial node extracellular matrix are distinct from contractile left ventricular matrix.
[So] Source:PLoS One;12(9):e0185125, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Extracellular matrix plays a role in differentiation and phenotype development of its resident cells. Although cardiac extracellular matrix from the contractile tissues has been studied and utilized in tissue engineering, extracellular matrix properties of the pacemaking sinoatrial node are largely unknown. In this study, the biomechanical properties and biochemical composition and distribution of extracellular matrix in the sinoatrial node were investigated relative to the left ventricle. Extracellular matrix of the sinoatrial node was found to be overall stiffer than that of the left ventricle and highly heterogeneous with interstitial regions composed of predominantly fibrillar collagens and rich in elastin. The extracellular matrix protein distribution suggests that resident pacemaking cardiomyocytes are enclosed in fibrillar collagens that can withstand greater tensile strength while the surrounding elastin-rich regions may undergo deformation to reduce the mechanical strain in these cells. Moreover, basement membrane-associated adhesion proteins that are ligands for integrins were of low abundance in the sinoatrial node, which may decrease force transduction in the pacemaking cardiomyocytes. In contrast to extracellular matrix of the left ventricle, extracellular matrix of the sinoatrial node may reduce mechanical strain and force transduction in pacemaking cardiomyocytes. These findings provide the criteria for a suitable matrix scaffold for engineering biopacemakers.
[Mh] Termos MeSH primário: Matriz Extracelular/metabolismo
Ventrículos do Coração/metabolismo
Nó Sinoatrial/metabolismo
[Mh] Termos MeSH secundário: Animais
Membrana Basal/química
Membrana Basal/metabolismo
Membrana Basal/ultraestrutura
Relógios Biológicos/fisiologia
Fenômenos Biomecânicos
Colágeno/metabolismo
Colágeno/ultraestrutura
Elasticidade
Elastina/metabolismo
Elastina/ultraestrutura
Matriz Extracelular/química
Matriz Extracelular/ultraestrutura
Fibronectinas/metabolismo
Fibronectinas/ultraestrutura
Imunofluorescência
Ventrículos do Coração/química
Ventrículos do Coração/ultraestrutura
Espectrometria de Massas
Microscopia de Força Atômica
Microscopia Eletroquímica de Varredura
Miócitos Cardíacos/química
Miócitos Cardíacos/metabolismo
Proteoma
Proteômica
Nó Sinoatrial/química
Nó Sinoatrial/ultraestrutura
Suínos
Resistência à Tração
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fibronectins); 0 (Proteome); 9007-34-5 (Collagen); 9007-58-3 (Elastin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170922
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185125


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[PMID]:28919421
[Au] Autor:Kim YJ; Yoo SM; Park HH; Lim HJ; Kim YL; Lee S; Seo KW; Kang KS
[Ad] Endereço:Stem Cells and Regenerative Bioengineering Institute, Kangstem Biotech CO., LTD., 2nd Floor, Biotechnology Center, #81 Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, South Korea.
[Ti] Título:Exosomes derived from human umbilical cord blood mesenchymal stem cells stimulates rejuvenation of human skin.
[So] Source:Biochem Biophys Res Commun;493(2):1102-1108, 2017 Nov 18.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Human umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) play an important role in cutaneous wound healing, and recent studies suggested that MSC-derived exosomes activate several signaling pathways, which are conducive in wound healing and cell growth. In this study, we investigated the roles of exosomes that are derived from USC-CM (USC-CM Exos) in cutaneous collagen synthesis and permeation. We found that USC-CM has various growth factors associated with skin rejuvenation. Our in vitro results showed that USC-CM Exos integrate in Human Dermal Fibroblasts (HDFs) and consequently promote cell migration and collagen synthesis of HDFs. Moreover, we evaluated skin permeation of USC-CM Exos by using human skin tissues. Results showed that Exo-Green labeled USC-CM Exos approached the outermost layer of the epidermis after 3 h and gradually approached the epidermis after 18 h. Moreover, increased expressions of Collagen I and Elastin were found after 3 days of treatment on human skin. The results showed that USC-CM Exos is absorbed into human skin, it promotes Collagen I and Elastin synthesis in the skin, which are essential to skin rejuvenation and shows the potential of USC-CM integration with the cosmetics or therapeutics.
[Mh] Termos MeSH primário: Exossomos/metabolismo
Sangue Fetal/citologia
Peptídeos e Proteínas de Sinalização Intercelular/metabolismo
Peptídeos e Proteínas de Sinalização Intercelular/farmacologia
Células Mesenquimais Estromais/metabolismo
Rejuvenescimento
Fenômenos Fisiológicos da Pele
[Mh] Termos MeSH secundário: Adulto
Células Cultivadas
Colágeno/metabolismo
Cosméticos
Elastina/metabolismo
Exossomos/química
Feminino
Sangue Fetal/química
Sangue Fetal/metabolismo
Fibroblastos/citologia
Fibroblastos/efeitos dos fármacos
Fibroblastos/metabolismo
Seres Humanos
Peptídeos e Proteínas de Sinalização Intercelular/isolamento & purificação
Peptídeos e Proteínas de Sinalização Intercelular/farmacocinética
Células Mesenquimais Estromais/química
Células Mesenquimais Estromais/citologia
Absorção Cutânea
Fenômenos Fisiológicos da Pele/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cosmetics); 0 (Intercellular Signaling Peptides and Proteins); 9007-34-5 (Collagen); 9007-58-3 (Elastin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170919
[St] Status:MEDLINE



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