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[PMID]:28465177
[Au] Autor:Liu NN; Chi Z; Wang QQ; Hong J; Liu GL; Hu Z; Chi ZM
[Ad] Endereço:College of Marine Life Sciences, Ocean University of China, Yushan Road, No. 5, Qingdao, China.
[Ti] Título:Simultaneous production of both high molecular weight pullulan and oligosaccharides by Aureobasdium melanogenum P16 isolated from a mangrove ecosystem.
[So] Source:Int J Biol Macromol;102:1016-1024, 2017 Sep.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×10 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×10 and a pullulan titer was 65.3g/L. The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase. During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×10 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×10 . Most of the oligosaccharides produced by the yeast strain P16 cultivated in the compositionally changed medium had degree of polymerization of 4 and 5. Therefore, both of the high Mw pullulan and oligosaccharides with high levels were produced by the yeast strain P16.
[Mh] Termos MeSH primário: Ascomicetos/isolamento & purificação
Ascomicetos/metabolismo
Glucanos/biossíntese
Glucanos/química
Oligossacarídeos/biossíntese
Oligossacarídeos/química
Zonas Úmidas
[Mh] Termos MeSH secundário: Fermentação
Peso Molecular
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucans); 0 (Oligosaccharides); 8ZQ0AYU1TT (pullulan)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


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[PMID]:28453664
[Au] Autor:Krag TO; Ruiz-Ruiz C; Vissing J
[Ad] Endereço:Copenhagen Neuromuscular Center, Department of Neurology, Rigshospitalet, University of Copenhagen, 2100 Copenhagen, Denmark.
[Ti] Título:Glycogen Synthesis in Glycogenin 1-Deficient Patients: A Role for Glycogenin 2 in Muscle.
[So] Source:J Clin Endocrinol Metab;102(8):2690-2700, 2017 Aug 01.
[Is] ISSN:1945-7197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Context: Glycogen storage disease (GSD) type XV is a rare disease caused by mutations in the GYG1 gene that codes for the core molecule of muscle glycogen, glycogenin 1. Nonetheless, glycogen is present in muscles of glycogenin 1-deficient patients, suggesting an alternative for glycogen buildup. A likely candidate is glycogenin 2, an isoform expressed in the liver and heart but not in healthy skeletal muscle. Objective: We wanted to investigate the formation of glycogen and changes in glycogen metabolism in patients with GSD type XV. Design, Setting, and Patients: Two patients with mutations in the GYG1 gene were investigated for histopathology, ultrastructure, and expression of proteins involved in glycogen synthesis and metabolism. Results: Apart from occurrence of polyglucosan (PG) bodies in few fibers, glycogen appeared normal in most cells, and the concentration was normal in patients with GSD type XV. We found that glycogenin 1 was absent, but glycogenin 2 was present in the patients, whereas the opposite was the case in healthy controls. Electron microscopy revealed that glycogen was present between and not inside myofibrils in type II fibers, compromising the ultrastructure of these fibers, and only type I fibers contained PG bodies. We also found significant changes to the expression levels of several enzymes directly involved in glycogen and glucose metabolism. Conclusions: To our knowledge, this is the first report demonstrating expression of glycogenin 2 in glycogenin 1-deficient patients, suggesting that glycogenin 2 rescues the formation of glycogen in patients with glycogenin 1 deficiency.
[Mh] Termos MeSH primário: Glucosiltransferases/genética
Glucosiltransferases/metabolismo
Glicogênio/biossíntese
Glicoproteínas/genética
Músculo Esquelético/metabolismo
[Mh] Termos MeSH secundário: Idoso
Metabolismo dos Carboidratos
Estudos de Casos e Controles
Feminino
Glucanos/metabolismo
Glucose/metabolismo
Glicogênio/metabolismo
Glicogênio/ultraestrutura
Doença de Depósito de Glicogênio/genética
Glicoproteínas/metabolismo
Seres Humanos
Microscopia Eletrônica
Meia-Idade
Fibras Musculares de Contração Rápida/ultraestrutura
Músculo Esquelético/patologia
Músculo Esquelético/ultraestrutura
Miofibrilas/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucans); 0 (Glycoproteins); 0 (glycogenin); 9005-79-2 (Glycogen); 9012-72-0 (polyglucosan); EC 2.4.1.- (Glucosyltransferases); EC 2.4.1.186 (GYG2 protein, human); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1210/jc.2017-00399


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[PMID]:29360855
[Au] Autor:Carrillo JB; Gomez-Casati DF; Martín M; Busi MV
[Ad] Endereço:Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Universidad Nacional de Rosario, Rosario, Santa Fe, Argentina.
[Ti] Título:Identification and analysis of OsttaDSP, a phosphoglucan phosphatase from Ostreococcus tauri.
[So] Source:PLoS One;13(1):e0191621, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ostreococcus tauri, the smallest free-living (non-symbiotic) eukaryote yet described, is a unicellular green alga of the Prasinophyceae family. It has a very simple cellular organization and presents a unique starch granule and chloroplast. However, its starch metabolism exhibits a complexity comparable to higher plants, with multiple enzyme forms for each metabolic reaction. Glucan phosphatases, a family of enzymes functionally conserved in animals and plants, are essential for normal starch or glycogen degradation in plants and mammals, respectively. Despite the importance of O. tauri microalgae in evolution, there is no information available concerning the enzymes involved in reversible phosphorylation of starch. Here, we report the molecular cloning and heterologous expression of the gene coding for a dual specific phosphatase from O. tauri (OsttaDSP), homologous to Arabidopsis thaliana LSF2. The recombinant enzyme was purified to electrophoretic homogeneity to characterize its oligomeric and kinetic properties accurately. OsttaDSP is a homodimer of 54.5 kDa that binds and dephosphorylates amylopectin. Also, we also determined that residue C162 is involved in catalysis and possibly also in structural stability of the enzyme. Our results could contribute to better understand the role of glucan phosphatases in the metabolism of starch in green algae.
[Mh] Termos MeSH primário: Clorófitas/enzimologia
Glucanos/metabolismo
Monoéster Fosfórico Hidrolases/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Clonagem Molecular
Dimerização
Cinética
Monoéster Fosfórico Hidrolases/química
Monoéster Fosfórico Hidrolases/genética
Fosforilação
Conformação Proteica
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Glucans); 0 (Recombinant Proteins); EC 3.1.3.2 (Phosphoric Monoester Hydrolases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180124
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191621


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[PMID]:29251503
[Au] Autor:Ge S; Li M; Ji N; Liu J; Mul H; Xiong L; Sun Q
[Ad] Endereço:College of Food Science and Engineering and ‡Central Laboratory, Qingdao Agricultural University Qingdao, Shandong Province 266109, China.
[Ti] Título:Preparation of a Strong Gelatin-Short Linear Glucan Nanocomposite Hydrogel by an in Situ Self-Assembly Process.
[So] Source:J Agric Food Chem;66(1):177-186, 2018 Jan 10.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gelatin hydrogels exhibit excellent biocompatibility, nonimmunogenicity, and biodegradability, but they have limited applications in the food and medical industries because of their poor mechanical properties. Herein, we first developed an in situ self-assembly process for the preparation of gelatin-short linear glucan (SLG) nanocomposite hydrogels with enhanced mechanical strength. The microstructure, dynamic viscoelasticity, compression behavior, and thermal characteristics of the gelatin-SLG nanocomposite hydrogels were determined using scanning electron microscopy (SEM), dynamic rheological experiments, compression tests, and texture profile analysis tests. The SEM images revealed that nanoparticles were formed by the in situ self-assembly of SLG in the gelatin matrix and that the size of these nanoparticles ranged between 200 and 600 nm. The pores of the nanocomposite hydrogels were smaller than those of the pure gelatin hydrogels. Transmission electron microscopy images and X-ray diffraction further confirmed the presence of SLG nanoparticles with spherical shapes and B-type structures. Compared with pure gelatin hydrogels, the nanocomposite hydrogels exhibited improved mechanical behavior. Notably, the hardness and maximum values of the compressive stress of gelatin-SLG nanocomposites containing 5% SLG increased by about 2-fold and 3-fold, respectively, compared to the corresponding values of pure gelatin hydrogels.
[Mh] Termos MeSH primário: Gelatina/química
Glucanos/química
Hidrogéis/química
Nanocompostos/química
[Mh] Termos MeSH secundário: Varredura Diferencial de Calorimetria
Difusão Dinâmica da Luz
Dureza
Microscopia Eletrônica de Varredura
Microscopia Eletrônica de Transmissão
Nanocompostos/ultraestrutura
Nanopartículas/química
Reologia
Espectroscopia de Infravermelho com Transformada de Fourier
Viscosidade
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucans); 0 (Hydrogels); 9000-70-8 (Gelatin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04684


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[PMID]:29388544
[Au] Autor:Christiansen L; Bech PK; Schultz-Johansen M; Martens HJ; Stougaard P
[Ad] Endereço:Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark.
[Ti] Título:Colwellia echini sp. nov., an agar- and carrageenan-solubilizing bacterium isolated from sea urchin.
[So] Source:Int J Syst Evol Microbiol;68(2):687-691, 2018 Feb.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel bacterial strain, A3 , was isolated from the intestines of the sea urchin Strongylocentrotus droebachiensis collected in Øresund, Denmark. The strain was Gram-reaction-negative, rod-shaped and facultatively anaerobic, and displayed growth at 5-25 °C (optimum 20 °C), pH 7-9 (optimum at pH 7) and 1-6 % (w/v) NaCl (optimum 3 %). Furthermore, strain A3 grew on agar, agarose, κ-carrageenan, alginate and laminarin as sole carbon source. Complete liquefaction of agar and κ-carrageenan was observed on solid plate media as a result of enzymatic activities. Major fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. The respiratory quinones were determined to be ubiquinones Q-8 (92 %) and Q-7 (8 %), and polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was 36.9 mol%. Phylogenetical analyses based on the 16S rRNA gene showed that the bacterium was affiliated with the genus Colwellia within the Alteromonadaceae of the Gammaproteobacteria. The level of 16S rRNA gene sequence similarity between strain A3 and its closest relatives in the genus Colwellia (C. psychrerythraea ATCC 27364 and C. asteriadis KMD 002 ) was 97.5 %. The average nucleotide identity between strain A3 and other members of Colwellia was 78.6-80.5 %, and DNA-DNA hybridization prediction revealed values of less than 23 % relatedness between strain A3 and other Colwellia species. The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain A3 represents a novel species of the genus Colwellia, for which the name Colwellia echini sp. nov. is proposed. The type strain is A3 (=LMG 30125 =NCIMB 15095 ).
[Mh] Termos MeSH primário: Alteromonadaceae/classificação
Filogenia
Strongylocentrotus/microbiologia
[Mh] Termos MeSH secundário: Ágar
Alginatos
Alteromonadaceae/genética
Alteromonadaceae/isolamento & purificação
Animais
Técnicas de Tipagem Bacteriana
Composição de Bases
Carragenina
DNA Bacteriano/genética
Dinamarca
Ácidos Graxos/química
Gammaproteobacteria
Glucanos
Ácido Glucurônico
Ácidos Hexurônicos
Fosfatidiletanolaminas/química
Fosfatidilgliceróis/química
RNA Ribossômico 16S/genética
Sefarose
Análise de Sequência de DNA
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alginates); 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Glucans); 0 (Hexuronic Acids); 0 (Phosphatidylethanolamines); 0 (Phosphatidylglycerols); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); 39382-08-6 (phosphatidylethanolamine); 8A5D83Q4RW (Glucuronic Acid); 8C3Z4148WZ (alginic acid); 9000-07-1 (Carrageenan); 9002-18-0 (Agar); 9008-22-4 (laminaran); 9012-36-6 (Sepharose); CQA993F7P8 (ubiquinone 8); RRK47DEG6Q (ubiquinone 7)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180202
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002568


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[PMID]:29293326
[Au] Autor:Ide M; Okumura M; Koizumi K; Kumagai M; Yoshida I; Yoshida M; Mishima T; Nakamura M
[Ad] Endereço:Japan Food Research Laboratories , Osaka 567-0085, Japan.
[Ti] Título:Novel Method to Quantify ß-Glucan in Processed Foods: Sodium Hypochlorite Extracting and Enzymatic Digesting (SEED) Assay.
[So] Source:J Agric Food Chem;66(4):1033-1038, 2018 Jan 31.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Some ß-glucans have attracted attention due to their functionality as an immunostimulant and have been used in processed foods. However, accurately measuring the ß-glucan content of processed foods using existing methods is difficult. We demonstrate a new method, the Sodium hypochlorite Extracting and Enzymatic Digesting (SEED) assay, in which ß-glucan is extracted using sodium hypochlorite, dimethyl sulfoxide, and 5 mol/L sodium hydroxide and then digested into ß-glucan fragments using Westase which is an enzyme having ß-1,6- and ß-1,3 glucanase activity. The ß-glucan fragments are further digested into glucose using exo-1,3-ß-d-glucanase and ß-glucosidase. We measured ß-glucan comprising ß-1,3-, -1,6-, and -1,(3),4- bonds in various polysaccharide reagents and processed foods using our novel method. The SEED assay was able to quantify ß-glucan with good reproducibility, and the recovery rate was >90% for food containing ß-glucan. Therefore, the SEED assay is capable of accurately measuring the ß-glucan content of processed foods.
[Mh] Termos MeSH primário: Análise de Alimentos/métodos
Hipoclorito de Sódio
beta-Glucanas/análise
[Mh] Termos MeSH secundário: Manipulação de Alimentos
Glucana 1,3-beta-Glucosidase/metabolismo
Glucanos/química
Hordeum/química
beta-Glucosidase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucans); 0 (beta-Glucans); 9008-22-4 (laminaran); DY38VHM5OD (Sodium Hypochlorite); EC 3.2.1.21 (beta-Glucosidase); EC 3.2.1.58 (Glucan 1,3-beta-Glucosidase); EC 3.2.1.58 (beta-1,3-exoglucanase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b05044


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[PMID]:27775129
[Au] Autor:Shimada R; Fujita M; Yuasa M; Sawamura H; Watanabe T; Nakashima A; Suzuki K
[Ad] Endereço:Department of Dietary Environment Analysis, School of Human Science and Environment, University of Hyogo, 1-1-12 Sinzaike Honcho, Himeji 670-0092, Japan. r-shimada@osaka-aoyama.ac.jp and Department of Health and Nutrition, Faculty of Health Science, Osaka Aoyama University, 2-11-1 Niina, Minoh, Osak
[Ti] Título:Oral administration of green algae, Euglena gracilis, inhibits hyperglycemia in OLETF rats, a model of spontaneous type 2 diabetes.
[So] Source:Food Funct;7(11):4655-4659, 2016 Nov 09.
[Is] ISSN:2042-650X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In the present study, the effects of Euglena and paramylon on hyperglycemia were examined in Otsuka Long-Evans Tokushima fatty (OLETF; type 2 diabetes mellitus model) rats. OLETF rats were fed an AIN-93 M diet containing cellulose, Euglena, or paramylon for 10 weeks. Long-Evans Tokushima Otsuka (LETO) rats were used as nondiabetic controls. An oral glucose-tolerance test (OGTT) was performed at 0 and 10 weeks. OLETF control rats were obese because of bulimia and showed abdominal fat accumulation and hyperglycemia. Euglena supplementation improved hyperglycemia and decreased food intake, body weight gain, and abdominal fat. However, there were no changes in the paramylon-supplemented group compared to the OLETF control group. Triglyceride concentrations in the serum and liver were lower in Euglena-supplemented rats than in OLETF control rats. There was a correlation between hepatic triglyceride concentration and the area under the curve (AUC) of OGTT at 10 weeks. This suggests that the improvement in glycemic control in the Euglena-supplemented group may depend on substances other than paramylon present in Euglena.
[Mh] Termos MeSH primário: Diabetes Mellitus Tipo 2/dietoterapia
Euglena gracilis
[Mh] Termos MeSH secundário: Administração Oral
Animais
Glicemia
Celulose/administração & dosagem
Celulose/farmacologia
Dieta
Glucanos/administração & dosagem
Glucanos/farmacologia
Teste de Tolerância a Glucose
Metabolismo dos Lipídeos
Masculino
Ratos
Ratos Endogâmicos OLETF
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Glucose); 0 (Glucans); 51052-65-4 (paramylon); 9004-34-6 (Cellulose)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:28946308
[Au] Autor:Yan J; Han Z; Qu Y; Yao C; Shen D; Tai G; Cheng H; Zhou Y
[Ad] Endereço:Jilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China.
[Ti] Título:Structure elucidation and immunomodulatory activity of a ß-glucan derived from the fruiting bodies of Amillariella mellea.
[So] Source:Food Chem;240:534-543, 2018 Feb 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel polysaccharide AAMP-A70 (5.6kDa) has been purified from the fruiting bodies of Amillariella mellea. Compositional analysis and 1H and 13C NMR spectra indicate that AAMP-A70 is a branched ß-glucan with a main chain that consists of ß-d-(1→6) linked Glcρ residues substituted at O-3 by ß-Glcρ or α-d-(1→6)-linked Galρ side chains. AAMP-A70 increases macrophage phagocytosis and secretion of NO, ROS, TNF-α, IL-6 and IL-1ß. Mechanistically, AAMP-A70 promotes degradation of IκB-α and nuclear translocation of the NF-κB p65 subunit, and enhances phosphorylation of MAPKs. In particular, the function blocking antibody to TLR2 substantially suppresses TNF-α and IL-6 production. Our data demonstrate that AAMP-A70 activates macrophages via NF-κB/MAPK signaling pathways and the TLR2 receptor. Overall, AAMP-A70 may serve as a good food supplement to enhance immunity.
[Mh] Termos MeSH primário: Agaricales
[Mh] Termos MeSH secundário: Carpóforos
Glucanos
NF-kappa B
Transdução de Sinais
beta-Glucanas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucans); 0 (NF-kappa B); 0 (beta-Glucans)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170927
[St] Status:MEDLINE


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[PMID]:29045474
[Au] Autor:Nakamura S; Kunikata T; Matsumoto Y; Hanaya T; Harashima A; Nishimoto T; Ushio S
[Ad] Endereço:R&D Center, Hayashibara Co., Ltd., Okayama, Japan.
[Ti] Título:Effects of a non-cyclodextrin cyclic carbohydrate on mouse melanoma cells: Characterization of a new type of hypopigmenting sugar.
[So] Source:PLoS One;12(10):e0186640, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cyclic nigerosyl nigerose (CNN) is a cyclic tetrasaccharide that exhibits properties distinct from other conventional cyclodextrins. Herein, we demonstrate that treatment of B16 melanoma with CNN results in a dose-dependent decrease in melanin synthesis, even under conditions that stimulate melanin synthesis, without significant cytotoxity. The effects of CNN were prolonged for more than 27 days, and were gradually reversed following removal of CNN. Undigested CNN was found to accumulate within B16 cells at relatively high levels. Further, CNN showed a weak but significant direct inhibitory effect on the enzymatic activity of tyrosinase, suggesting one possible mechanism of hypopigmentation. While a slight reduction in tyrosinase expression was observed, tyrosinase expression was maintained at significant levels, processed into a mature form, and transported to late-stage melanosomes. Immunocytochemical analysis demonstrated that CNN treatment induced drastic morphological changes of Pmel17-positive and LAMP-1-positive organelles within B16 cells, suggesting that CNN is a potent organelle modulator. Colocalization of both tyrosinase-positive and LAMP-1-positive regions in CNN-treated cells indicated possible degradation of tyrosinase in LAMP-1-positive organelles; however, that possibility was ruled out by subsequent inhibition experiments. Taken together, this study opens a new paradigm of functional oligosaccharides, and offers CNN as a novel hypopigmenting molecule and organelle modulator.
[Mh] Termos MeSH primário: Ciclodextrinas/farmacologia
Glucanos/farmacologia
Hipopigmentação/patologia
Melanoma Experimental/patologia
[Mh] Termos MeSH secundário: Animais
Western Blotting
Linhagem Celular Tumoral
Glucosamina/farmacologia
Imuno-Histoquímica
Lisossomos/efeitos dos fármacos
Lisossomos/metabolismo
Melaninas/biossíntese
Melanoma Experimental/metabolismo
Melanossomas/efeitos dos fármacos
Melanossomas/metabolismo
Camundongos
Monofenol Mono-Oxigenase/metabolismo
Pressão Osmótica
Estresse Fisiológico/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclodextrins); 0 (Glucans); 0 (Melanins); 0 (cyclic nigerosylnigerose); EC 1.14.18.1 (Monophenol Monooxygenase); N08U5BOQ1K (Glucosamine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171019
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186640


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Texto completo
[PMID]:28974421
[Au] Autor:Nakashima A; Suzuki K; Asayama Y; Konno M; Saito K; Yamazaki N; Takimoto H
[Ad] Endereço:euglena Co., Ltd., 5-33-1 Shiba, Minato-ku, Tokyo, 108-0014, Japan.
[Ti] Título:Oral administration of Euglena gracilis Z and its carbohydrate storage substance provides survival protection against influenza virus infection in mice.
[So] Source:Biochem Biophys Res Commun;494(1-2):379-383, 2017 Dec 09.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Euglena gracilis Z is a micro-algae that is used as a food or nutritional supplement. Paramylon, the carbohydrate storage substance of Euglena gracilis Z has ß-1, 3-glucan structure. Euglena gracilis Z and paramylon are reported to affect the immune system. In this study, we investigated the protective effects of Euglena gracilis Z and paramylon against influenza virus infection in mice. Euglena gracilis Z and paramylon were administered to mice as a 2% dietary mixture ad libitum. At 2 weeks after initiation of dietary administration, mice were infected intranasally with influenza virus A/PR/8/34 (H1N1). Survival rate was monitored 10 days after infection. In addition, we performed virus titer and cytokine profiles in the lung. High survival rates were observed for Euglena gracilis Z and paramylon-treated groups compared to the control group. Significantly lower virus titer in the lung was observed in the Euglena gracilis Z and paramylon-treated groups compared to the control group from day 1 after infection. Higher amount of IL-1ß, IL-6, IL-12 (p70), IFN-γ, and IL-10 was observed in the paramylon groups compared to the control group. Our data therefore reveals a novel immunoregulatory role of the Euglena gracilis Z and paramylon which provides protection against influenza virus infection.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos/administração & dosagem
Suplementos Nutricionais
Euglena gracilis/imunologia
Glucanos/administração & dosagem
Pulmão/efeitos dos fármacos
Infecções por Orthomyxoviridae/dietoterapia
[Mh] Termos MeSH secundário: Administração Oral
Animais
Euglena gracilis/química
Feminino
Vírus da Influenza A Subtipo H1N1/efeitos dos fármacos
Vírus da Influenza A Subtipo H1N1/crescimento & desenvolvimento
Vírus da Influenza A Subtipo H1N1/patogenicidade
Interferon gama/biossíntese
Interferon gama/imunologia
Interleucina-10/biossíntese
Interleucina-10/imunologia
Interleucina-12/biossíntese
Interleucina-12/imunologia
Interleucina-1beta/biossíntese
Interleucina-1beta/imunologia
Interleucina-6/biossíntese
Interleucina-6/imunologia
Pulmão/imunologia
Pulmão/virologia
Camundongos
Camundongos Endogâmicos BALB C
Infecções por Orthomyxoviridae/imunologia
Infecções por Orthomyxoviridae/mortalidade
Infecções por Orthomyxoviridae/virologia
Análise de Sobrevida
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Glucans); 0 (IL10 protein, mouse); 0 (IL1B protein, mouse); 0 (Interleukin-1beta); 0 (Interleukin-6); 130068-27-8 (Interleukin-10); 187348-17-0 (Interleukin-12); 51052-65-4 (paramylon); 82115-62-6 (Interferon-gamma)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171005
[St] Status:MEDLINE



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