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  1 / 7820 MEDLINE  
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[PMID]:28460247
[Au] Autor:Zweckmair T; Schiehser S; Rosenau T; Potthast A
[Ad] Endereço:Division of Chemistry of Renewable Resources, University of Natural Resources and Life Sciences, Konrad-Lorenz-Strasse 24, A-3430 Tulln, Austria.
[Ti] Título:Improved quantification of monosaccharides in complex lignocellulosic biomass matrices: A gas chromatography-mass spectrometry based approach.
[So] Source:Carbohydr Res;446-447:7-12, 2017 Jun 29.
[Is] ISSN:1873-426X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A novel method for the precise and accurate quantification of wood monosaccharides by gas-chromatography mass-spectrometry in complex lignocellulosic biomass matrices is presented. Instead of using the syn- or the anti-peak obtained by blocking the anomeric center using e.g. oximation, the sum of each syn- and anti-peak pair is used for quantification rendering the approach of the apparently constant syn- and anti-peak area ratio. Each wood monosaccharide syn- and anti-peak could essentially be distinguished upon O-ethoximation followed by trifluoroacetylation and separation on a 14% cyanopropyl/phenyl- 86% dimethylpoly-siloxane column. Additionally, internal standardization was carried out applying isotopically labeled compounds. Hence, the analytical figures of merit, such as precision and accuracy, could be substantially improved compared to standard gas-chromatography mass spectrometry as well as high-performance anion exchange chromatography (HPAEC) coupled to pulsed-amperometric detection (PAD). The applicability of the novel gas-chromatography mass-spectrometry approach is demonstrated by analysis of five selected lignocellulosic biomass sample matrices typically occurring in a biorefinery scenario. Even in exceedingly complex sample matrices precise and accurate data are obtained according to the methodology presented.
[Mh] Termos MeSH primário: Biomassa
Cromatografia Gasosa-Espectrometria de Massas
Lignina/química
Monossacarídeos/análise
[Mh] Termos MeSH secundário: Madeira/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Monosaccharides); 11132-73-3 (lignocellulose); 9005-53-2 (Lignin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  2 / 7820 MEDLINE  
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[PMID]:29241332
[Au] Autor:Rencoret J; Kim H; Evaristo AB; Gutiérrez A; Ralph J; Del Río JC
[Ad] Endereço:Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS), CSIC , Av. Reina Mercedes, 10, 41012 Seville, Spain.
[Ti] Título:Variability in Lignin Composition and Structure in Cell Walls of Different Parts of Macaúba (Acrocomia aculeata) Palm Fruit.
[So] Source:J Agric Food Chem;66(1):138-153, 2018 Jan 10.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The lignins from different anatomical parts of macaúba (Acrocomia aculeata) palm fruit, namely stalks, epicarp, and endocarp, were studied. The lignin from stalks was enriched in S-lignin units (S/G 1.2) and ß-ether linkages (84% of the total) and was partially acylated at the γ-OH of the lignin side-chains (26% lignin acylation), predominantly with p-hydroxybenzoates and acetates. The epicarp lignin was highly enriched in G-lignin units (S/G 0.2) and consequently depleted in ß-ethers (65%) and enriched in condensed structures such as phenylcoumarans (24%) and dibenzodioxocins (3%). The endocarp lignin was strikingly different from the rest and presented large amounts of piceatannol units incorporated into the polymer. This resulted in a lignin polymer depleted in ß-ethers but enriched in condensed structures and linked piceatannol moieties. The incorporation of piceatannol into the lignin polymer seems to have a role in seed protection.
[Mh] Termos MeSH primário: Arecaceae/citologia
Parede Celular/química
Lignina/química
[Mh] Termos MeSH secundário: Acilação
Arecaceae/química
Parede Celular/metabolismo
Cromatografia em Gel
Cromatografia Gasosa-Espectrometria de Massas
Lignina/análise
Espectroscopia de Ressonância Magnética
Peso Molecular
Polissacarídeos/análise
Polissacarídeos/química
Estilbenos/análise
Estilbenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Polysaccharides); 0 (Stilbenes); 6KS3LS0D4F (3,3',4,5'-tetrahydroxystilbene); 9005-53-2 (Lignin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04638


  3 / 7820 MEDLINE  
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[PMID]:28463479
[Au] Autor:Hong CY; Ryu SH; Jeong H; Lee SS; Kim M; Choi IG
[Ad] Endereço:Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science , Seoul, Republic of Korea.
[Ti] Título:Phanerochaete chrysosporium Multienzyme Catabolic System for in Vivo Modification of Synthetic Lignin to Succinic Acid.
[So] Source:ACS Chem Biol;12(7):1749-1759, 2017 Jul 21.
[Is] ISSN:1554-8937
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Whole cells of the basidiomycete fungus Phanerochaete chrysosporium (ATCC 20696) were applied to induce the biomodification of lignin in an in vivo system. Our results indicated that P. chrysosporium has a catabolic system that induces characteristic biomodifications of synthetic lignin through a series of redox reactions, leading not only to the degradation of lignin but also to its polymerization. The reducing agents ascorbic acid and α-tocopherol were used to stabilize the free radicals generated from the ligninolytic process. The application of P. chrysosporium in combination with reducing agents produced aromatic compounds and succinic acid as well as degraded lignin polymers. P. chrysosporium selectively catalyzed the conversion of lignin to succinic acid, which has an economic value. A transcriptomic analysis of P. chrysosporium suggested that the bond cleavage of synthetic lignin was caused by numerous enzymes, including extracellular enzymes such as lignin peroxidase and manganese peroxidase, and that the aromatic compounds released were metabolized in both the short-cut and classical tricarboxylic acid cycles of P. chrysosporium. In conclusion, P. chrysosporium is suitable as a biocatalyst for lignin degradation to produce a value-added product.
[Mh] Termos MeSH primário: Lignina/metabolismo
Complexos Multienzimáticos/química
Phanerochaete/enzimologia
Ácido Succínico/síntese química
[Mh] Termos MeSH secundário: Ácido Ascórbico/química
Radicais Livres
Lignina/química
Peso Molecular
Nitrobenzenos/química
Oxirredução
Phanerochaete/metabolismo
Ácido Succínico/química
Ácido Succínico/metabolismo
Tocoferóis/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Free Radicals); 0 (Multienzyme Complexes); 0 (Nitrobenzenes); 9005-53-2 (Lignin); AB6MNQ6J6L (Succinic Acid); E57JCN6SSY (nitrobenzene); PQ6CK8PD0R (Ascorbic Acid); R0ZB2556P8 (Tocopherols)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1021/acschembio.7b00046


  4 / 7820 MEDLINE  
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[PMID]:28745867
[Au] Autor:DeLorenzo DM; Henson WR; Moon TS
[Ad] Endereço:Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis , St. Louis, Missouri 63130, United States.
[Ti] Título:Development of Chemical and Metabolite Sensors for Rhodococcus opacus PD630.
[So] Source:ACS Synth Biol;6(10):1973-1978, 2017 Oct 20.
[Is] ISSN:2161-5063
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Rhodococcus opacus PD630 is a nonmodel, Gram-positive bacterium that possesses desirable traits for biomass conversion, including consumption capabilities for lignocellulose-based sugars and toxic lignin-derived aromatic compounds, significant triacylglycerol accumulation, relatively rapid growth rate, and genetic tractability. However, few genetic elements have been directly characterized in R. opacus, limiting its application for lignocellulose bioconversion. Here, we report the characterization and development of genetic tools for tunable gene expression in R. opacus, including: (1) six fluorescent reporters for quantifying promoter output, (2) three chemically inducible promoters for variable gene expression, and (3) two classes of metabolite sensors derived from native R. opacus promoters that detect nitrogen levels or aromatic compounds. Using these tools, we also provide insights into native aromatic consumption pathways in R. opacus. Overall, this work expands the ability to control and characterize gene expression in R. opacus for future lignocellulose-based fuel and chemical production.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/métodos
Rhodococcus/genética
[Mh] Termos MeSH secundário: Lignina/metabolismo
Nitrogênio/metabolismo
Regiões Promotoras Genéticas/genética
Rhodococcus/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9005-53-2 (Lignin); N762921K75 (Nitrogen)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1021/acssynbio.7b00192


  5 / 7820 MEDLINE  
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[PMID]:29228039
[Au] Autor:Liu B; Olson Å; Wu M; Broberg A; Sandgren M
[Ad] Endereço:Department of Molecular Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
[Ti] Título:Biochemical studies of two lytic polysaccharide monooxygenases from the white-rot fungus Heterobasidion irregulare and their roles in lignocellulose degradation.
[So] Source:PLoS One;12(12):e0189479, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lytic polysaccharide monooxygenases (LPMO) are important redox enzymes produced by microorganisms for the degradation of recalcitrant natural polysaccharides. Heterobasidion irregulare is a white-rot phytopathogenic fungus that causes wood decay in conifers. The genome of this fungus encodes 10 putative Auxiliary Activity family 9 (AA9) LPMOs. We describe the first biochemical characterization of H. irregulare LPMOs through heterologous expression of two CBM-containing LPMOs from this fungus (HiLPMO9H, HiLPMO9I) in Pichia pastoris. The oxidization preferences and substrate specificities of these two enzymes were determined. The two LPMOs were shown to cleave different carbohydrate components of plant cell walls. HiLPMO9H was active on cellulose and oxidized the substrate at the C1 carbon of the pyranose ring at ß-1,4-glycosidic linkages, whereas HiLPMO9I cleaved cellulose with strict oxidization at the C4 carbon of glucose unit at internal bonds, and also showed activity against glucomannan. We propose that the two LPMOs play different roles in the plant-cell-wall degrading system of H. irregulare for degradation of softwood and that the lignocellulose degradation mediated by this white-rot fungus may require collective efforts from multi-types of LPMOs.
[Mh] Termos MeSH primário: Basidiomycota/enzimologia
Lignina/metabolismo
Oxigenases de Função Mista/metabolismo
Polissacarídeos/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Cromatografia Líquida de Alta Pressão
Cromatografia por Troca Iônica
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas
Oxigenases de Função Mista/química
Pichia/genética
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Polysaccharides); 11132-73-3 (lignocellulose); 9005-53-2 (Lignin); EC 1.- (Mixed Function Oxygenases)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189479


  6 / 7820 MEDLINE  
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[PMID]:28833149
[Au] Autor:He Y; Mouthier TM; Kabel MA; Dijkstra J; Hendriks WH; Struik PC; Cone JW
[Ad] Endereço:Animal Nutrition Group, Wageningen University & Research, 6700 AH Wageningen, the Netherlands.
[Ti] Título:Lignin composition is more important than content for maize stem cell wall degradation.
[So] Source:J Sci Food Agric;98(1):384-390, 2018 Jan.
[Is] ISSN:1097-0010
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The relationship between the chemical and molecular properties - in particular the (acid detergent) lignin (ADL) content and composition expressed as the ratio between syringyl and guaiacyl compounds (S:G ratio) - of maize stems and in vitro gas production was studied in order to determine which is more important in the degradability of maize stem cell walls in the rumen of ruminants. Different internodes from two contrasting maize cultivars (Ambrosini and Aastar) were harvested during the growing season. RESULTS: The ADL content decreased with greater internode number within the stem, whereas the ADL content fluctuated during the season for both cultivars. The S:G ratio was lower in younger tissue (greater internode number or earlier harvest date) in both cultivars. For the gas produced between 3 and 20 h, representing the fermentation of cell walls in rumen fluid, a stronger correlation (R = 0.80) was found with the S:G ratio than with the ADL content (R = 0.68). The relationship between ADL content or S:G ratio and 72-h gas production, representing total organic matter degradation, was weaker than that with gas produced between 3 and 20 h. CONCLUSION: The S:G ratio plays a more dominant role than ADL content in maize stem cell wall degradation. © 2017 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
[Mh] Termos MeSH primário: Parede Celular/metabolismo
Lignina/química
Caules de Planta/metabolismo
Ruminantes/metabolismo
Zea mays/metabolismo
[Mh] Termos MeSH secundário: Animais
Parede Celular/química
Lignina/metabolismo
Caules de Planta/química
Rúmen/metabolismo
Zea mays/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9005-53-2 (Lignin)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171228
[Lr] Data última revisão:
171228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170824
[St] Status:MEDLINE
[do] DOI:10.1002/jsfa.8630


  7 / 7820 MEDLINE  
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[PMID]:28451936
[Au] Autor:Zhang W; Wu L; Ding Y; Yao X; Wu X; Weng F; Li G; Liu Z; Tang S; Ding C; Wang S
[Ad] Endereço:Jiangsu Collaborative Innovation Center for Modern Crop Production/National Engineering and Technology Center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China, Nanjing Agricultural University, Nanjing, 210095, China.
[Ti] Título:Nitrogen fertilizer application affects lodging resistance by altering secondary cell wall synthesis in japonica rice (Oryza sativa).
[So] Source:J Plant Res;130(5):859-871, 2017 Sep.
[Is] ISSN:1618-0860
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Stem mechanical strength is an important agricultural quantitative trait that is closely related to lodging resistance in rice, which is known to be reduced by fertilizer with higher levels of nitrogen. To understand the mechanism that regulates stem mechanical strength in response to nitrogen, we analysed stem morphology, anatomy, mechanical properties, cell wall components, and expression of cell wall-related genes, in two varieties of japonica rice, namely, Wuyunjing23 (lodging-resistant variety) and W3668 (lodging-susceptible variety). The results showed that higher nitrogen fertilizer increased the lodging index in both varieties due to a reduction in breaking strength and bending stress, and these changes were larger in W3668. Cellulose content decreased slightly under higher nitrogen fertilizer, whereas lignin content reduced remarkably. Histochemical staining revealed that high nitrogen application decreased lignin deposition in the secondary cell wall of the sclerenchyma cells and vascular bundle cells compared with the low nitrogen treatments, while it did not alter the pattern of cellulose deposition in these cells in both Wuyunjing23 and W3668. In addition, the expression of the genes involved in lignin biosynthesis, OsPAL, OsCoMT, Os4CL3, OsCCR, OsCAD2, OsCAD7, OsCesA4, and OsCesA7, were also down-regulated under higher nitrogen conditions at the early stage of culm growth. These results suggest that the genes involved in lignin biosynthesis are down-regulated by higher nitrogen fertilizer, which causes lignin deficiency in the secondary cell walls and the weakening of mechanical tissue structure. Subsequently, this results in these internodes with reduced mechanical strength and poor lodging resistance.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Lignina/biossíntese
Nitrogênio/farmacologia
Oryza/efeitos dos fármacos
[Mh] Termos MeSH secundário: Parede Celular/efeitos dos fármacos
Parede Celular/metabolismo
Parede Celular/ultraestrutura
Celulose/metabolismo
Fertilizantes
Lignina/ultraestrutura
Nitrogênio/metabolismo
Oryza/genética
Oryza/metabolismo
Oryza/ultraestrutura
Fenótipo
Caules de Planta/efeitos dos fármacos
Caules de Planta/genética
Caules de Planta/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fertilizers); 9004-34-6 (Cellulose); 9005-53-2 (Lignin); N762921K75 (Nitrogen)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171228
[Lr] Data última revisão:
171228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1007/s10265-017-0943-3


  8 / 7820 MEDLINE  
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[PMID]:28466110
[Au] Autor:Kabel MA; Jurak E; Mäkelä MR; de Vries RP
[Ad] Endereço:Laboratory of Food Chemistry, Wageningen University, Bornse Weilanden 9, 6708 WG, Wageningen, The Netherlands.
[Ti] Título:Occurrence and function of enzymes for lignocellulose degradation in commercial Agaricus bisporus cultivation.
[So] Source:Appl Microbiol Biotechnol;101(11):4363-4369, 2017 Jun.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The white button mushroom Agaricus bisporus is economically the most important commercially produced edible fungus. It is grown on carbon- and nitrogen-rich substrates, such as composted cereal straw and animal manure. The commercial mushroom production process is usually performed in buildings or tunnels under highly controlled environmental conditions. In nature, the basidiomycete A. bisporus has a significant impact on the carbon cycle in terrestrial ecosystems as a saprotrophic decayer of leaf litter. In this mini-review, the fate of the compost plant cell wall structures, xylan, cellulose and lignin, is discussed. A comparison is made from the structural changes observed to the occurrence and function of enzymes for lignocellulose degradation present, with a special focus on the extracellular enzymes produced by A. bisporus. In addition, recent advancements in whole genome level molecular studies in various growth stages of A. bisporus in compost are reviewed.
[Mh] Termos MeSH primário: Agaricus/enzimologia
Celulose/metabolismo
Lignina/metabolismo
Xilanos/metabolismo
[Mh] Termos MeSH secundário: Agaricus/genética
Agaricus/crescimento & desenvolvimento
Animais
Carbono/metabolismo
Ciclo do Carbono
Genoma Fúngico
Micélio/crescimento & desenvolvimento
Nitrogênio/metabolismo
Solo
Xilanos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Soil); 0 (Xylans); 11132-73-3 (lignocellulose); 7440-44-0 (Carbon); 9004-34-6 (Cellulose); 9005-53-2 (Lignin); N762921K75 (Nitrogen)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171220
[Lr] Data última revisão:
171220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-017-8294-5


  9 / 7820 MEDLINE  
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[PMID]:29220020
[Au] Autor:Sarria S; Kruyer NS; Peralta-Yahya P
[Ad] Endereço:School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA.
[Ti] Título:Microbial synthesis of medium-chain chemicals from renewables.
[So] Source:Nat Biotechnol;35(12):1158-1166, 2017 Dec.
[Is] ISSN:1546-1696
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Linear, medium-chain (C8-C12) hydrocarbons are important components of fuels as well as commodity and specialty chemicals. As industrial microbes do not contain pathways to produce medium-chain chemicals, approaches such as overexpression of endogenous enzymes or deletion of competing pathways are not available to the metabolic engineer; instead, fatty acid synthesis and reversed ß-oxidation are manipulated to synthesize medium-chain chemical precursors. Even so, chain lengths remain difficult to control, which means that purification must be used to obtain the desired products, titers of which are typically low and rarely exceed milligrams per liter. By engineering the substrate specificity and activity of the pathway enzymes that generate the fatty acyl intermediates and chain-tailoring enzymes, researchers can boost the type and yield of medium-chain chemicals. Development of technologies to both manipulate chain-tailoring enzymes and to assay for products promises to enable the generation of g/L yields of medium-chain chemicals.
[Mh] Termos MeSH primário: Biocombustíveis
Biomassa
Ácidos Graxos/metabolismo
Hidrocarbonetos/metabolismo
Engenharia Metabólica/métodos
[Mh] Termos MeSH secundário: Escherichia coli
Lignina
Saccharomyces cerevisiae
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Biofuels); 0 (Fatty Acids); 0 (Hydrocarbons); 11132-73-3 (lignocellulose); 9005-53-2 (Lignin)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171220
[Lr] Data última revisão:
171220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE
[do] DOI:10.1038/nbt.4022


  10 / 7820 MEDLINE  
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[PMID]:29088238
[Au] Autor:Zhang J; Cheng X; Jin Q; Su X; Li M; Yan C; Jiao X; Li D; Lin Y; Cai Y
[Ad] Endereço:School of Life Science, Anhui Agricultural University, Hefei, China.
[Ti] Título:Comparison of the transcriptomic analysis between two Chinese white pear (Pyrus bretschneideri Rehd.) genotypes of different stone cells contents.
[So] Source:PLoS One;12(10):e0187114, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Stone cell content is thought to be one of the key determinants for fruit quality in pears. However, the molecular mechanism of stone cell development remains poorly understood. In this study, we found that the stone cell clusters (SCCs) distribution and area in 'Dangshan Su' (with abundant stone cells) were higher as compared to 'Lianglizaosu' (low stone cell content bud sport of 'Dangshan Su') based on the histochemical staining, and the correlations of lignin content with stone cell content and SCC area was significant. The fruits of 'Dangshan Su' and 'Lianglizaosu' at three different developmental stages (23 and 55 days after flowering and mature) were sampled for comparative transcriptome analysis to explore the metabolic pathways associated with stone cell development. A total of 42444 unigenes were obtained from two varieties, among which 7203 differentially expressed genes (DEGs) were identified by comparison of the six transcriptomes. Specifically, many DEGs associated with lignin biosynthesis were identified, including coumaroylquinate 3-monooxygenase (C3H), shikimate O-hydroxycinnamoyltransferase (HCT), ferulate 5-hydroxylase (F5H), cinnamyl alcohol dehydrogenase (CAD) and peroxidase (POD), as well as genes related to carbon metabolism, such as sorbitol dehydrogenase-like (SDH-like) and ATP-dependent 6-phosphofructokinase (ATP-PFK). At the peak of the stone cell content (55 days after flowering), the expression level of these genes in 'Dangshan Su' was significantly increased compared with 'Lianglizaosu', indicating that these genes were closely related to stone cell development. We validated the transcriptional levels of 33 DEGs using quantitative real-time polymerase chain reaction (qRT-PCR) analysis. The results were consistent with the transcriptome analysis, indicating the reliability of transcriptome data. In addition, subcellular localization analysis of three DEGs in lignin synthesis (PbC3H, PbF5H and PbPOD) revealed that these proteins are mainly distributed in the cell membrane and cytoplasm. These results provide new insights into the molecular mechanism of stone cell formation.
[Mh] Termos MeSH primário: Frutas/citologia
Pyrus/genética
[Mh] Termos MeSH secundário: Frutas/crescimento & desenvolvimento
Frutas/metabolismo
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Estudos de Associação Genética
Lignina/metabolismo
Redes e Vias Metabólicas/genética
Pyrus/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Análise de Sequência de DNA
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
9005-53-2 (Lignin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171101
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0187114



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