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  1 / 20321 MEDLINE  
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[PMID]:29208260
[Au] Autor:Fu T; Wang L; Zeng Q; Zhang Y; Sheng B; Han L
[Ad] Endereço:Department of Respiration, the 1st People's Hospital of Jining, Jining, China.
[Ti] Título:Ghrelin Ameliorates Asthma by Inhibiting Endoplasmic Reticulum Stress.
[So] Source:Am J Med Sci;354(6):617-625, 2017 12.
[Is] ISSN:1538-2990
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: This study aimed to confirm the ameliorative effect of ghrelin on asthma and investigate its mechanism. MATERIALS AND METHODS: The murine model of asthma was induced by ovalbumin (OVA) treatment and assessed by histological pathology and airway responsiveness to methacholine. The total and differential leukocytes were counted. Tumor necrosis factor α, interferon γ, interleukin-5 and interleukin-13 levels in bronchoalveolar lavage fluid were quantified by commercial kits. The protein levels in pulmonary tissues were measured by Western blot analysis. RESULTS: Ghrelin ameliorated the histological pathology and airway hyperresponsiveness in the OVA-induced asthmatic mouse model. Consistently, OVA-increased total and differential leukocytes and levels of tumor necrosis factor α, interferon γ, interleukin-5 and interleukin-13 in bronchoalveolar lavage fluid were significantly attenuated by ghrelin. Ghrelin prevented the increased protein levels of the endoplasmic reticulum stress markers glucose regulated protein 78 and CCAAT/enhancer binding protein homologous protein and reversed the reduced levels of p-Akt in asthmatic mice. CONCLUSIONS: Ghrelin might prevent endoplasmic reticulum stress activation by stimulating the Akt signaling pathway, which attenuated inflammation and ameliorated asthma in mice. Ghrelin might be a new target for asthma therapy.
[Mh] Termos MeSH primário: Antiasmáticos/uso terapêutico
Asma/tratamento farmacológico
Retículo Endoplasmático/efeitos dos fármacos
Grelina/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Antiasmáticos/farmacologia
Asma/patologia
Asma/fisiopatologia
Western Blotting
Líquido da Lavagem Broncoalveolar/química
Líquido da Lavagem Broncoalveolar/citologia
Modelos Animais de Doenças
Grelina/farmacologia
Queratinas/análise
Pulmão/efeitos dos fármacos
Pulmão/patologia
Masculino
Cloreto de Metacolina/farmacologia
Camundongos
Camundongos Endogâmicos C57BL
Estresse Fisiológico/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Asthmatic Agents); 0 (Ghrelin); 0W5ETF9M2K (Methacholine Chloride); 68238-35-7 (Keratins)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE


  2 / 20321 MEDLINE  
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[PMID]:29374706
[Au] Autor:Ceausu AR; Ciolofan A; Cimpean AM; Magheti A; Mederle O; Raica M
[Ad] Endereço:Department of Microscopic Morphology/Histology, Angiogenesis Research Center Timisoara, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania.
[Ti] Título:The Mesenchymal-Epithelial and Epithelial-Mesenchymal Cellular Plasticity of Liver Metastases with Digestive Origin.
[So] Source:Anticancer Res;38(2):811-816, 2018 02.
[Is] ISSN:1791-7530
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Few data are available regarding the epithelial to mesenchymal transition (EMT) /mesenchymal to epitheilal transition (MET) in the liver metastasis of digestive cancers. The aim of this study was to establish EMT/MET metastatic tumor cell plasticity according to the histological growth pattern of liver metastases. MATERIALS AND METHODS: Biopsies from 25 patients with liver metastasis (desmoplastic, replacement and pushing type) were evaluated. Double immunostaining of E-cadherin/vimentin, keratin 8,18/vimentin and E-cadherin/ keratin 8,18 were performed. RESULTS: The following cell types were noted: epithelial, mesenchymal, non-differentiated and differentiated hybrid mesenchymal/ epithelial and non-hybrid phenotype. All cases had mesenchymal/ epithelial phenotype cells. A significant correlation was found between the non-differentiated hybrid mesenchymal/ epithelial phenotype metastatic cells and histological growth pattern for gastric and colorectal cancer. CONCLUSION: A MET-targeting strategy, in conjunction with conventional chemotherapy, may be useful for the treatment of liver metastases.
[Mh] Termos MeSH primário: Neoplasias do Sistema Digestório/patologia
Neoplasias Hepáticas/secundário
[Mh] Termos MeSH secundário: Caderinas/metabolismo
Plasticidade Celular/fisiologia
Neoplasias Colorretais/metabolismo
Neoplasias Colorretais/patologia
Neoplasias do Sistema Digestório/metabolismo
Transição Epitelial-Mesenquimal/fisiologia
Seres Humanos
Imuno-Histoquímica
Queratinas/metabolismo
Neoplasias Hepáticas/metabolismo
Neoplasias Hepáticas/patologia
Neoplasias Pancreáticas/metabolismo
Neoplasias Pancreáticas/patologia
Neoplasias Gástricas/metabolismo
Neoplasias Gástricas/patologia
Vimentina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CDH1 protein, human); 0 (Cadherins); 0 (Vimentin); 68238-35-7 (Keratins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180129
[St] Status:MEDLINE


  3 / 20321 MEDLINE  
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[PMID]:29374702
[Au] Autor:Ferreira L; Vitorino R; Neuparth MJ; Rodrigues D; Gama A; Faustino-Rocha AI; Ferreira R; Oliveira PA
[Ad] Endereço:Organic Chemistry, Natural Products and Foodstuffs (QOPNA), Mass Spectrometry Center, University of Aveiro, Aveiro, Portugal.
[Ti] Título:Intense Pulsed Light: Friend or Foe? Molecular Evidence to Clarify Doubts.
[So] Source:Anticancer Res;38(2):779-786, 2018 02.
[Is] ISSN:1791-7530
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIM: Intense pulsed light (IPL) has been extensively applied in the field of dermatology and aesthetics; however, the long-term consequences of its use are poorly unknown, and to the best of our knowledge there is no study on the effect of IPL in neoplastic lesions. In order to better understand the molecular mechanisms underlying IPL application in the skin, we used an animal model of carcinogenesis obtained by chemical induction with 12-dimethylbenz(a)anthracene (DMBA) and 12-O-tetradecanoylphorbol-13-acetate (TPA). MATERIALS AND METHODS: Institute of Cancer Research (ICR) mice were administered DMBA and/or TPA and treated with IPL. Skin was evaluated by histopathology and 2DE-blot-MS/MS analysis. RESULTS: Our data evidenced an inflammatory response and a metabolic remodeling of skin towards a glycolytic phenotype after chronic exposure to IPL, which was accomplished by increased oxidative stress and susceptibility to apoptosis. These alterations induced by IPL were more notorious in the DMBA sensitized skin. Keratins and metabolic proteins seem to be the more susceptible to oxidative modifications that might result in loss of function, contributing for the histological changes observed in treated skin. CONCLUSION: Data highlight the deleterious impact of IPL on skin phenotype, which justifies the need for more experimental studies in order to increase our understanding of the IPL long-term safety.
[Mh] Termos MeSH primário: Terapia de Luz Pulsada Intensa/efeitos adversos
Neoplasias Cutâneas/etiologia
Pele/efeitos da radiação
[Mh] Termos MeSH secundário: 9,10-Dimetil-1,2-benzantraceno/administração & dosagem
Animais
Apoptose/efeitos dos fármacos
Apoptose/efeitos da radiação
Carcinógenos/administração & dosagem
Modelos Animais de Doenças
Feminino
Glicólise
Queratinas/metabolismo
Camundongos
Camundongos Endogâmicos ICR
Estresse Oxidativo/efeitos dos fármacos
Estresse Oxidativo/efeitos da radiação
Distribuição Aleatória
Pele/efeitos dos fármacos
Pele/metabolismo
Pele/patologia
Neoplasias Cutâneas/induzido quimicamente
Neoplasias Cutâneas/metabolismo
Neoplasias Cutâneas/patologia
Acetato de Tetradecanoilforbol/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carcinogens); 57-97-6 (9,10-Dimethyl-1,2-benzanthracene); 68238-35-7 (Keratins); NI40JAQ945 (Tetradecanoylphorbol Acetate)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180129
[St] Status:MEDLINE


  4 / 20321 MEDLINE  
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[PMID]:29320551
[Au] Autor:Navarro-Lozano A; Sánchez-Domene D; Rossa-Feres DC; Bosch J; Sawaya RJ
[Ad] Endereço:Departamento de Zoologia e Botânica. Universidade Estadual Paulista, São José do Rio Preto, São Paulo, Brazil.
[Ti] Título:Are oral deformities in tadpoles accurate indicators of anuran chytridiomycosis?
[So] Source:PLoS One;13(1):e0190955, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We evaluated the use of oral deformities as reliable proxies for determining Batrachochytrium dendrobatidis (Bd) infection in tadpoles of six anuran species of the Atlantic Forest in southeastern Brazil. We examined oral discs of 2156 tadpoles of six species of anurans collected in 2016: Aplastodiscus albosignatus, Boana albopunctata, Boana faber, Scinax hayii, Crossodactylus caramaschii, and Physalaemus cuvieri. Three oral deformities were recognized: lack of keratinization only in upper and/or lower jaw sheaths, lack of keratinization only in upper or lower tooth rows, and both deformities together. A subsample composed of all the individuals possessing oral deformities (N = 195) plus randomly selected individuals without oral deformities (N = 184) were tested for Bd via qPCR. Oral deformities were observed in all six species, but only five were infected with Bd. Since we found that dekeratinization of tooth rows was not associated with the presence of Bd in any of the studied species we used a new proxy (jaw sheaths dekeratinization with or without dekeratinization in tooth rows: JSD-proxy) for Bd detection. Our results showed a nonrandom relationship between Bd infection and JSD-proxy in three species of the family Hylidae. However, the use of JSD-proxy for Bd detection in these species resulted in up to 30.8% false positives and up to 29.3% false negatives. The use of the JSD-proxy in species for which no relationship was found reached 100% of false positives. We conclude that the use of oral dekeratinization as a generalized proxy for Bd detection in tadpoles should not be used as a single diagnosis technique.
[Mh] Termos MeSH primário: Anuros/microbiologia
Quitridiomicetos
Larva/microbiologia
Boca/patologia
Micoses/veterinária
[Mh] Termos MeSH secundário: Animais
Reações Falso-Negativas
Reações Falso-Positivas
Queratinas
Micoses/diagnóstico
Micoses/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
68238-35-7 (Keratins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190955


  5 / 20321 MEDLINE  
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[PMID]:29224278
[Au] Autor:Zhao M; LaoI QY; Zhao DH; Ma J; Ru GQ; He XL; Wang Z; Wang J
[Ad] Endereço:Department of Pathology, Zhejiang Provincial People's Hospital, Hangzhou 310014, China.
[Ti] Título:[Clinicopathologic and molecular genetic characterizations of biphenotypic sinonasal sarcoma].
[So] Source:Zhonghua Bing Li Xue Za Zhi;46(12):841-846, 2017 Dec 08.
[Is] ISSN:0529-5807
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To investigate the clinicopathologic characteristics, immunophenotypes, molecular genetics, and diagnostic and differential diagnostic features of biphenotypic sinonasal sarcoma (BSNS). Three cases of BSNS were retrieved, the histomorphology, immunophenotype and molecular genetics were analyzed with review of literature. There were 2 male and 1 female patient aged 45, 29 and 40 years, respectively.Computed tomography and magnetic resonance imaging examinations showed a large polypoid mass occupying the sinonasal cavity in all 3 patients. Microscopically, these tumors were un-circumscribed and composed of cellular spindle-shaped cells arranged in long and interlaced fascicles. A hemangiopericytoma-like growth pattern was frequently identified. The overlying hyperplastic respiratory epithelium invaginated down into the tumor forming a cystic (2 cases), glandular (1 case) structures and inverted in a papilloma-like (1 case)pattern, and foci of eosinophilic metaplasia were also noted in 2 of the three cases. The tumor nuclei were bland-appearing, mitoses were scarce and necrosis was absent. Immunohistochemically, the tumor cells showed co-expression of neural and myogenic markers in all the 3 cases, including that 3/3 showed diffuse and strong positivity of S-100 protein, 3/3 positivity of smooth muscle actin (1 diffuse and 2 focal), 1/2 diffuse positivity of calponin, 1/3 focal positivity of desmin, and 1/1 focal positivity of MyoD1.In addition, 1 detected for ß-catenin showed focal nuclear positivity. None of the 3 showed positivity to cytokeratin, CD34 or SOX10 in the tumor cells.Ki-67 showed an index <5%, 10% and <2%, respectively. Fluorescence in situ hybridization analysis showed rearrangements of PAX3 gene in all 3 cases. In case 3, reverse transcription polymerase chain reaction, followed by Sanger sequencing, demonstrated an in-frame fusion between PAX3 and FOXO1.Follow-up information (range 3-15 months)showed no evidence of local recurrence or distant metastasis in three cases. BSNS is a newly described entity which can be readily confused with a variety of benign and malignant spindle cell tumors encountered in the sinonasal cavity; immunohistochemistry co-expression of neural and myogenic markers and PAX3 gene rearrangement can help distinguish this tumor from its many mimickers.
[Mh] Termos MeSH primário: Neoplasias dos Seios Paranasais/genética
Neoplasias dos Seios Paranasais/patologia
Sarcoma/genética
Sarcoma/patologia
[Mh] Termos MeSH secundário: Adulto
Biomarcadores Tumorais/análise
Núcleo Celular
Desmina/análise
Diagnóstico Diferencial
Feminino
Rearranjo Gênico
Hemangiopericitoma/patologia
Seres Humanos
Imuno-Histoquímica
Imunofenotipagem
Hibridização in Situ Fluorescente
Queratinas/análise
Masculino
Meia-Idade
Recidiva Local de Neoplasia
Fator de Transcrição PAX3/genética
Neoplasias dos Seios Paranasais/química
Neoplasias dos Seios Paranasais/imunologia
Proteínas S100/análise
Fatores de Transcrição SOXE/análise
Sarcoma/química
Sarcoma/imunologia
beta Catenina/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (CTNNB1 protein, human); 0 (Desmin); 0 (PAX3 Transcription Factor); 0 (PAX3 protein, human); 0 (S100 Proteins); 0 (SOXE Transcription Factors); 0 (beta Catenin); 68238-35-7 (Keratins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.0529-5807.2017.12.006


  6 / 20321 MEDLINE  
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[PMID]:28832260
[Au] Autor:Davoudi Z; Rabiee M; Houshmand B; Eslahi N; Khoshroo K; Rasoulianboroujeni M; Tahriri M; Tayebi L
[Ad] Endereço:a Biomaterials Group, Faculty of Biomedical Engineering , Amirkabir University of Technology , Tehran , Iran.
[Ti] Título:Development of chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis.
[So] Source:Drug Dev Ind Pharm;44(1):40-55, 2018 Jan.
[Is] ISSN:1520-5762
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this research was to develop chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate as a buccal mucoadhesive patch to treat desquamative gingivitis, which was fabricated through an environmental friendly process. Mucoadhesive films increase the advantage of higher efficiency and drug localization in the affected region. In this research, mucoadhesive films, for the release of hydrocortisone sodium succinate, were prepared using different ratios of chitosan, gelatin and keratin. In the first step, chitosan and gelatin proportions were optimized after evaluating the mechanical properties, swelling capacity, water uptake, stability, and biodegradation of the films. Then, keratin was added at different percentages to the optimum composite of chitosan and gelatin together with the drug. The results of surface pH showed that none of the samples were harmful to the buccal cavity. FTIR analysis confirmed the influence of keratin on the structure of the composite. The presence of a higher amount of keratin in the composite films resulted in high mechanical, mucoadhesive properties and stability, low water uptake and biodegradation in phosphate buffer saline (pH = 7.4) containing 10 U/ml lysozyme. The release profile of the films ascertained that keratin is a rate controller in the release of the hydrocortisone sodium succinate. Finally, chitosan/gelatin/keratin composite containing hydrocortisone sodium succinate can be employed in dental applications.
[Mh] Termos MeSH primário: Quitosana/química
Gelatina/química
Gengivite/tratamento farmacológico
Hidrocortisona/análogos & derivados
Hidrocortisona/química
Queratinas/química
Succinatos/química
[Mh] Termos MeSH secundário: Adesividade
Hidrocortisona/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Succinates); 68238-35-7 (Keratins); 9000-70-8 (Gelatin); 9012-76-4 (Chitosan); LIU00Z1Z84 (cortisol succinate); WI4X0X7BPJ (Hydrocortisone)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180111
[Lr] Data última revisão:
180111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170824
[St] Status:MEDLINE
[do] DOI:10.1080/03639045.2017.1371738


  7 / 20321 MEDLINE  
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[PMID]:28957335
[Au] Autor:Boonkasidecha S; Kannan PS; Kallapur SG; Jobe AH; Kemp MW
[Ad] Endereço:Perinatal Institute, Cincinnati Children's Hospital Medical Center, University of Cincinnati School of Medicine, Cincinnati, Ohio, United States of America.
[Ti] Título:Fetal skin as a pro-inflammatory organ: Evidence from a primate model of chorioamnionitis.
[So] Source:PLoS One;12(9):e0184938, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Intrauterine infection is a primary cause of preterm birth and fetal injury. The pro-inflammatory role of the fetal skin in the setting of intrauterine infection remains poorly characterized. Whether or not inflammation of the fetal skin occurs in primates remains unstudied. Accordingly, we hypothesized that: i) the fetal primate skin would mount a pro-inflammatory response to preterm birth associated pro-inflammatory agents (lipopolysaccharides from Escherichia coli, live Ureaplasma parvum, interleukin-1ß) and; ii) that inhibiting interleukin-1 signaling would decrease the skin inflammatory response. METHODS: Rhesus macaques with singleton pregnancies received intraamniotic injections of either sterile saline (control) or one of three pro-inflammatory agonists: E. coli lipopolysaccharides, interluekin-1ß or live U. parvum under ultrasound guidance. A fourth group of animals received both E. coli lipopolysaccharide and interleukin-1 signaling inhibitor interleukin-1 receptor antagonist (Anakinra) prior to delivery. Animals were surgically delivered at approximately 130 days' gestational age. RESULTS: Intraamniotic lipopolysaccharide caused an inflammatory skin response characterized by increases in interluekin-1ß,-6 and -8 mRNA at 16 hours. There was a modest inflammatory response to U. parvum, but interleukin-1ß alone caused no inflammatory response in the fetal skin. Intraamniotic Anakinra treatment of lipopolysaccharide-exposed animals significantly reduced skin inflammation. CONCLUSIONS: Intraamniotic lipopolysaccharide and U. parvum were associated with modest increases in the expression of inflammatory mediators in primate fetal skin. Although administration of Interleukin-1ß alone did not elicit an inflammatory response, lipopolysaccharide-driven skin inflammation was decreased following intraamniotic Anakinra therapy. These findings provide support for the role of the fetal skin in the development of the fetal inflammatory response.
[Mh] Termos MeSH primário: Corioamnionite/patologia
Feto/patologia
Inflamação/patologia
Pele/patologia
[Mh] Termos MeSH secundário: Animais
Corioamnionite/genética
Modelos Animais de Doenças
Feminino
Inflamação/complicações
Inflamação/genética
Mediadores da Inflamação/metabolismo
Interleucina-1beta/genética
Interleucina-1beta/metabolismo
Interleucina-8/genética
Interleucina-8/metabolismo
Queratinas/metabolismo
Lipopolissacarídeos
Macaca mulatta
Masculino
Reação em Cadeia da Polimerase
Gravidez
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Ureaplasma/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Inflammation Mediators); 0 (Interleukin-1beta); 0 (Interleukin-8); 0 (Lipopolysaccharides); 0 (RNA, Messenger); 68238-35-7 (Keratins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170929
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184938


  8 / 20321 MEDLINE  
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[PMID]:28928080
[Au] Autor:Jiang M; Li B; Zhang J; Hu L; Dang E; Wang G
[Ad] Endereço:Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
[Ti] Título:Vascular endothelial growth factor driving aberrant keratin expression pattern contributes to the pathogenesis of psoriasis.
[So] Source:Exp Cell Res;360(2):310-319, 2017 Nov 15.
[Is] ISSN:1090-2422
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Psoriasis is a chronic inflammatory skin disease severely affecting patients' physical and psychological well-being. Aberrant keratin expression in psoriasis plays a crucial role in keratinocyte dysfunction and disease development. Little is yet known about the mechanism of this keratin dysregulation. VEGF (Vascular endothelial growth factor) is significantly elevated in psoriatic patients and VEGFRs are detected on keratinocytes, leading to our hypothesis that this keratin dysregulation may be regulated by VEGF. In this study, we showed that VEGFR2 was overexpressed in psoriatic epidermis and was correlated with K (keratin) 6, K16&K17 upregulation and K1&K10 downregulation. VEGF increased both mRNA and protein levels of K6, K16&K17 and decreased those of K1&K10 in NHEKs (normal human epidermal keratinocytes). Further we identified activation of STAT3, ERK1/2, and p38 pathways in VEGF-treated NHEKs. Using specific pathway antagonists and siRNAs we found that VEGF induced K6, K16&K17 via these three pathways and reduced K1&K10 via ERK1/2. Finally, VEGF-induced aberrant keratin expression pattern and epidermal thickening were confirmed in a VEGF-local-injection mouse model. Collectively, we demonstrated that VEGF was associated with aberrant keratin expression pattern in psoriasis and provided a new insight into the role of VEGF in psoriasis pathogenesis, indicating VEGF as a potential therapeutic target.
[Mh] Termos MeSH primário: Queratinas/genética
Psoríase/genética
Fator A de Crescimento do Endotélio Vascular/farmacologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Animais
Células Cultivadas
Criança
Epiderme/efeitos dos fármacos
Epiderme/metabolismo
Epiderme/patologia
Feminino
Perfilação da Expressão Gênica
Regulação da Expressão Gênica/efeitos dos fármacos
Seres Humanos
Queratinas/metabolismo
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Psoríase/patologia
Pele/efeitos dos fármacos
Pele/metabolismo
Pele/patologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Vascular Endothelial Growth Factor A); 68238-35-7 (Keratins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170921
[St] Status:MEDLINE


  9 / 20321 MEDLINE  
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[PMID]:28927164
[Au] Autor:Jeffus SK; Gardner JM; Steliga MA; Shah AA; Stelow EB; Arnaoutakis K
[Ad] Endereço:Department of Pathology.
[Ti] Título:Hyalinizing Clear Cell Carcinoma of the Lung: Case Report and Review of the Literature.
[So] Source:Am J Clin Pathol;148(1):73-80, 2017 Jul 01.
[Is] ISSN:1943-7722
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Objectives: Hyalinizing clear cell carcinoma (HCCC) is common in head and neck sites but extremely rare in the lung. This case report describes an HCCC in the lung of a 54-year-old female patient. Methods: We summarize the histomorphologic, immunophenotypic, and molecular features for our and three previously reported HCCCs of the lung with emphasis on potential diagnostic pitfalls. Results: Sections of a well-circumscribed 3.5-cm lung mass were characterized by a bronchocentric tumor growing in sheets, nests, and cords in a background of hyalinized stroma. Tumor cell appearance was clear to eosinophilic, lacking significant pleomorphism or mitotic activity. By immunohistochemistry, the tumor cells were strongly positive with antibodies to pan-keratin, p63, and CK5/6 while negative for CK7, CK20, thyroid transcription factor 1, napsin A, chromogranin, and synaptophysin. Next-generation sequencing demonstrated an EWSR1-ATF1 fusion transcript. Conclusions: Awareness of key morphologic features of pulmonary HCCC is crucial for the recognition of this rare entity in the lung. Ancillary studies, including immunohistochemistry and molecular testing, are essential for the distinction from its mimics.
[Mh] Termos MeSH primário: Adenocarcinoma de Células Claras/patologia
Neoplasias Pulmonares/patologia
[Mh] Termos MeSH secundário: Adenocarcinoma de Células Claras/metabolismo
Adenocarcinoma de Células Claras/cirurgia
Biomarcadores Tumorais/metabolismo
Feminino
Seres Humanos
Imuno-Histoquímica
Queratinas/metabolismo
Neoplasias Pulmonares/metabolismo
Neoplasias Pulmonares/cirurgia
Proteínas de Membrana/metabolismo
Meia-Idade
Proteínas de Fusão Oncogênicas/genética
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (CKAP4 protein, human); 0 (Membrane Proteins); 0 (Oncogene Proteins, Fusion); 68238-35-7 (Keratins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170921
[St] Status:MEDLINE
[do] DOI:10.1093/ajcp/aqx048


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[PMID]:28893547
[Au] Autor:Díaz-Hernández V; Vázquez-Gómez A; Marmolejo-Valencia A; Montaño LM; Merchant-Larios H
[Ad] Endereço:Departamento de Embriología, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México, Mexico. Electronic address: roveazdih@yahoo.com.mx.
[Ti] Título:17ß-Estradiol modulates cell proliferation of medullary cords during ovarian differentiation of the Lepidochelys olivacea sea turtle.
[So] Source:Dev Biol;431(2):263-271, 2017 11 15.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In turtles undergoing temperature sex determination (TSD), bipotential gonads express Sox9 in medullary cords at both female- (FPT) and male-producing temperatures (MPT). Subsequently, when the sex fate of medullary cords becomes dimorphic, at FPT, Sox9 is downregulated, whereas at MPT, its expression is maintained. Medullary cords in the ovary turn into ovarian lacuna, whereas in the testis they differentiate as seminiferous cords. When embryos of Lepidochelys olivacea sea turtle are incubated at MPT and treated with estradiol, Sox9 expression persists in the medullary cords in the form of tiny ovotestis-like formations. The perturbed development of the treated gonads is due to a significant decrease in the number of proliferating cells. This suggests that the disturbed effect caused by exogenous estradiol may be due to a conflict between the gene networks regulated by temperature and the increased level of endogenous estrogens, induced by the treatment. Here, we decided to use fadrozole and fulvestrant, an aromatase inhibitor and an estrogen-receptor antagonist, respectively, to provide insights into the role played by endogenous estrogens in regulating the cell proliferation of the two main gonadal compartments: the medullary cords and the cortex. Comparing cell proliferation patterns, our current results suggest that the endogenous estrogens are involved in determining the sex fate of medullary cords, by repressing proliferation. Interestingly, our results showed that endogenous estradiol levels are unnecessary for the thickening of the ovarian cortex.
[Mh] Termos MeSH primário: Estradiol/farmacologia
Ovário/citologia
Diferenciação Sexual/efeitos dos fármacos
Tartarugas/fisiologia
[Mh] Termos MeSH secundário: Animais
Proliferação Celular/efeitos dos fármacos
Embrião não Mamífero/efeitos dos fármacos
Embrião não Mamífero/metabolismo
Estradiol/análogos & derivados
Fadrozol/farmacologia
Feminino
Imunofluorescência
Queratinas/metabolismo
Masculino
Ovário/embriologia
Ovário/ultraestrutura
Fatores de Transcrição SOX9/metabolismo
Temperatura Ambiente
Testículo/efeitos dos fármacos
Tartarugas/embriologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (SOX9 Transcription Factor); 22X328QOC4 (fulvestrant); 4TI98Z838E (Estradiol); 68238-35-7 (Keratins); H3988M64PU (Fadrozole)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE



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