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  1 / 163 MEDLINE  
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[PMID]:28509589
[Au] Autor:Kang X; Liu Y; Zhang J; Xu Q; Liu C; Fang M
[Ad] Endereço:1 National Engineering Laboratory for Animal Breeding, MOA Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University , Beijing, People's Republic of China .
[Ti] Título:Characteristics and Expression Profile of KRT71 Screened by Suppression Subtractive Hybridization cDNA Library in Curly Fleece Chinese Tan Sheep.
[So] Source:DNA Cell Biol;36(7):552-564, 2017 Jul.
[Is] ISSN:1557-7430
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:As an important commercial trait for sheep, curly fleece has a great economic impact on production costs and efficiency in sheep industry. To identify genes that are important for curly fleece formation in mammals, a suppression subtractive hybridization analysis was performed on the shoulder skin tissues exposed to two different growth stages of Chinese Tan sheep with different phenotypes (curly fleece and noncurling fleece). BLAST analysis identified 67 differentially expressed genes, of which 31 were expressed lower and 36 were expressed higher in lambs than in adult sheep. Differential expressions of seven randomly selected genes were verified using quantitative real-time polymerase chain reaction (qRT-PCR). KRT71 gene was selected for further study due to its high correlation with the curly hair phenotype in various mammal species. Semi-qPCR showed distinctively high expression of KRT71 in skin tissues. Moreover, qPCR result showed a significantly higher expression of KRT71 in curly fleece than noncurling Tan sheep. The luciferase assay and electrophoresis mobility shift assay showed that there were transcription factor binding sites in the promoter region of KRT71 related to the differential expression of KRT71 at the two growth stages of Tan sheep. Online bioinformation tools predicted MFZ1 as a transcriptional factor that regulates the expression of KRT71. These studies on KRT71 gene revealed some mechanisms underlying the relationship between the KRT71 gene and the curly fleece phenotype of Tan sheep.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica no Desenvolvimento
Cabelo/metabolismo
Queratinas Específicas do Cabelo/genética
Característica Quantitativa Herdável
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Fatores Etários
Animais
Sítios de Ligação
Perfilação da Expressão Gênica
Biblioteca Gênica
Ontologia Genética
Genes Reporter
Cabelo/anatomia & histologia
Cabelo/crescimento & desenvolvimento
Seres Humanos
Queratinas Específicas do Cabelo/metabolismo
Luciferases/genética
Luciferases/metabolismo
Anotação de Sequência Molecular
Fenótipo
Regiões Promotoras Genéticas
Ligação Proteica
Carneiro Doméstico
Técnicas de Hibridização Subtrativa
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Keratins, Hair-Specific); 0 (Transcription Factors); EC 1.13.12.- (Luciferases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170517
[St] Status:MEDLINE
[do] DOI:10.1089/dna.2017.3718


  2 / 163 MEDLINE  
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[PMID]:28405679
[Au] Autor:Nanashima N; Horie K; Yamada T; Shimizu T; Tsuchida S
[Ad] Endereço:Department of Biomedical Sciences, Hirosaki University Graduate School of Health Sciences, Hirosaki 036-8564, Japan.
[Ti] Título:Hair keratin KRT81 is expressed in normal and breast cancer cells and contributes to their invasiveness.
[So] Source:Oncol Rep;37(5):2964-2970, 2017 May.
[Is] ISSN:1791-2431
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Keratins are fibrous proteins. Hair keratins constitute hard structures such as the hair and nails, and cytokeratins have been used as markers of breast carcinoma. However, the expression and function of full-size hair keratin genes have not been previously demonstrated in breast cancer. We investigated the expression of the hair keratin, KRT81, and its function in human breast cancer and normal mammary epithelial cells. Western blotting showed full size 55-kDa KRT81 expression in the human breast cancer cell lines, MCF7, SKBR3 and MDA-MB-231, normal human mammary epithelial cells (HMEC), and non-neoplastic cells (MCF10A). Reverse transcription-polymerase chain reaction revealed that the full size KRT81, including its 5' region is expressed in breast cells. Immunohistochemical and immunofluorescence analyses showed that KRT81 was located in the cytoplasm. To investigate the function of KRT81, we knocked down KRT81 by siRNA in MCF10A cells. Microarray analysis revealed that the expression of genes related to invasion such as matrix metallopeptidase (MMP)9 was decreased. In KRT81-knockdown MDA-MB231 cells, zymography revealed a decrease in MMP9 activity, while scratch and invasion assays revealed that KRT81-knockdown decreased cell migration and invasion abilities. This is the first study showing that full size KRT81 is expressed in normal breast epithelial cells and breast cancer cells. Moreover, our results indicate that KRT81 contributes to the migration and invasion of breast cancer cells.
[Mh] Termos MeSH primário: Neoplasias da Mama/metabolismo
Queratinas Específicas do Cabelo/genética
Queratinas Específicas do Cabelo/metabolismo
Queratinas Tipo II/genética
Queratinas Tipo II/metabolismo
Glândulas Mamárias Humanas/metabolismo
[Mh] Termos MeSH secundário: Neoplasias da Mama/genética
Linhagem Celular Tumoral
Movimento Celular
Citoplasma/metabolismo
Feminino
Regulação Neoplásica da Expressão Gênica
Seres Humanos
Células MCF-7
Invasividade Neoplásica
Análise de Sequência com Séries de Oligonucleotídeos/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (KRT81 protein, human); 0 (Keratins, Hair-Specific); 0 (Keratins, Type II)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170508
[Lr] Data última revisão:
170508
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.3892/or.2017.5564


  3 / 163 MEDLINE  
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[PMID]:28101861
[Au] Autor:Fraser RD; Parry DA
[Ad] Endereço:Institute of Fundamental Sciences, Massey University, Private Bag 11-222, Palmerston North, 4442, New Zealand.
[Ti] Título:Structural Transition of Trichocyte Keratin Intermediate Filaments During Development in the Hair Follicle.
[So] Source:Subcell Biochem;82:131-149, 2017.
[Is] ISSN:0306-0225
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The intermediate filaments (IF) in trichocyte (hard α-) keratin are unique amongst the various classes of IF in having not one but two topologically-distinct structures. The first is formed at an early stage of hair development in a reducing environment within the cells in the lower part of the follicle. The second structure occurs at a later stage of hair development in the upper part of the follicle, where there is a transition to an oxidizing environment. Crosslinking studies reveal that molecular slippage occurs within the IF upon oxidation and that this results in many cysteine residues lying in near axial alignment, thereby facilitating disulphide bond formation. The disulphide bonds so formed stabilize the assembly of IF molecules and convert the keratin fibre into a tough, resilient and insoluble structure suitable for its function in vivo as a thermo-regulator and a protector of the animal against its external environment.
[Mh] Termos MeSH primário: Queratinas Específicas do Cabelo/química
Queratinas Específicas do Cabelo/ultraestrutura
[Mh] Termos MeSH secundário: Animais
Folículo Piloso
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Keratins, Hair-Specific)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170619
[Lr] Data última revisão:
170619
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170120
[St] Status:MEDLINE
[do] DOI:10.1007/978-3-319-49674-0_5


  4 / 163 MEDLINE  
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[PMID]:26880085
[Au] Autor:Alibardi L
[Ad] Endereço:Comparative Histolab and Dipartimento di Biologia, Geologia e Scienze Ambientali, Università di Bologna, 40126, Bologna, Italy. lorenzo.alibardi@unibo.it.
[Ti] Título:Ultrastructural localization of hair keratins, high sulfur keratin-associated proteins and sulfhydryl oxidase in the human hair.
[So] Source:Anat Sci Int;92(2):248-261, 2017 Mar.
[Is] ISSN:1447-073X
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Hardening of the human hair shaft during cornification results from the bonding of keratins and keratin-associated proteins. In situ hybridization and light immunocytochemical studies have shown the general distribution of different keratins and some associated proteins but not determined their ultrastructural localization. I report here the localization of hair keratins, two high-sulfur keratin-associated proteins and sulfhydryl oxidase has been studied under the transmission electron microscope in the cornification zone of the human hair. The ultrastructural study on keratin distribution in general confirms previous light microscopic studies. Sulfur-rich KAP1 is mainly cortical but the labeling disappears in fully cornified cortical cells while a diffuse labeling is also present in differentiating cuticle cells. Sulfur-rich K26 immunolocalization is only detected in the exocuticle and endocuticle. Sparse labeling for sulfhydryl oxidase occurs in differentiating cortical cells but is weak and uneven in cuticle cells and absent in medulla and inner root sheath. Labeling disappears in the upper fully cornified cortex and cuticle. The observations indicate that sulfhydryl oxidase and keratin associated proteins are initially produced in the cytoplasm among keratin bundles accumulating in cortical and cuticle cells but these proteins undergo changes during the following cornification that alter the epitopes tagged by the antibodies.
[Mh] Termos MeSH primário: Folículo Piloso/ultraestrutura
Cabelo/ultraestrutura
Queratinas Específicas do Cabelo/ultraestrutura
Oxirredutases/ultraestrutura
[Mh] Termos MeSH secundário: Diferenciação Celular
Cabelo/metabolismo
Folículo Piloso/metabolismo
Seres Humanos
Queratinas Específicas do Cabelo/metabolismo
Oxirredutases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Keratins, Hair-Specific); EC 1.- (Oxidoreductases); EC 1.8.3.- (sulfhydryl oxidase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170313
[Lr] Data última revisão:
170313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160217
[St] Status:MEDLINE
[do] DOI:10.1007/s12565-016-0330-5


  5 / 163 MEDLINE  
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[PMID]:27100288
[Au] Autor:Zhao Z; Liu G; Li X; Huang J; Xiao Y; Du X; Yu M
[Ad] Endereço:Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
[Ti] Título:Characterization of the Promoter Regions of Two Sheep Keratin-Associated Protein Genes for Hair Cortex-Specific Expression.
[So] Source:PLoS One;11(4):e0153936, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The keratin-associated proteins (KAPs) are the structural proteins of hair fibers and are thought to play an important role in determining the physical properties of hair fibers. These proteins are activated in a striking sequential and spatial pattern in the keratinocytes of hair fibers. Thus, it is important to elucidate the mechanism that underlies the specific transcriptional activity of these genes. In this study, sheep KRTAP 3-3 and KRTAP11-1 genes were found to be highly expressed in wool follicles in a tissue-specific manner. Subsequently, the promoter regions of the two genes that contained the 5' flanking/5' untranslated regions and the coding regions were cloned. Using an in vivo transgenic approach, we found that the promoter regions from the two genes exhibited transcriptional activity in hair fibers. A much stronger and more uniformly expressed green fluorescent signal was observed in the KRTAP11-1-ZsGreen1 transgenic mice. In situ hybridization revealed the symmetrical expression of sheep KRTAP11-1 in the entire wool cortex. Consistently, immunohistochemical analysis demonstrated that the pattern of ZsGreen1 expression in the hair cortex of transgenic mice matches that of the endogenous KRTAP11-1 gene, indicating that the cloned promoter region contains elements that are sufficient to govern the wool cortex-specific transcription of KRTAP11-1. Furthermore, regulatory regions in the 5' upstream sequence of the sheep KRTAP11-1 gene that may regulate the observed hair keratinocyte specificity were identified using in vivo reporter assays.
[Mh] Termos MeSH primário: Biomarcadores/metabolismo
Regulação da Expressão Gênica
Cabelo/metabolismo
Queratinas Específicas do Cabelo/genética
Regiões Promotoras Genéticas/genética
[Mh] Termos MeSH secundário: Animais
Clonagem Molecular
Feminino
Perfilação da Expressão Gênica
Técnicas Imunoenzimáticas
Hibridização In Situ
Queratinas Específicas do Cabelo/metabolismo
Masculino
Camundongos
Camundongos Transgênicos
Filogenia
Ovinos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers); 0 (Keratins, Hair-Specific)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160430
[Lr] Data última revisão:
160430
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160422
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0153936


  6 / 163 MEDLINE  
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[PMID]:26902920
[Au] Autor:Zernov NV; Skoblov MY; Marakhonov AV; Shimomura Y; Vasilyeva TA; Konovalov FA; Abrukova AV; Zinchenko RA
[Ad] Endereço:Federal State Budgetary Institution "Research Centre for Medical Genetics," Moscow, Russia. Electronic address: nzernov01@gmail.com.
[Ti] Título:Autosomal Recessive Hypotrichosis with Woolly Hair Caused by a Mutation in the Keratin 25 Gene Expressed in Hair Follicles.
[So] Source:J Invest Dermatol;136(6):1097-105, 2016 Jun.
[Is] ISSN:1523-1747
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hypotrichosis is an abnormal condition characterized by decreased hair density and various defects in hair structure and growth patterns. In particular, in woolly hair, hypotrichosis is characterized by a tightly curled structure and abnormal growth. In this study, we present a detailed comparative examination of individuals affected by autosomal-recessive hypotrichosis (ARH), which distinguishes two types of ARH. Earlier, we demonstrated that exon 4 deletion in the lipase H gene caused an ARH (hypotrichosis 7; MIM: 604379) in populations of the Volga-Ural region of Russia. Screening for this mutation in all affected individuals revealed its presence only in the group with the hypotrichosis 7 phenotype. Other patients formed a separate group of woolly hair-associated ARH, with a homozygous missense mutation c.712G>T (p.Val238Leu) in a highly conserved position of type I keratin KRT25 (K25). Haplotype analysis indicated a founder effect. An expression study in the HaCaT cell line demonstrated a deleterious effect of the p.Val238Leu mutation on the formation of keratin intermediate filaments. Hence, we have identified a previously unreported missense mutation in the KRT25 gene causing ARH with woolly hair.
[Mh] Termos MeSH primário: Alopecia/congênito
Predisposição Genética para Doença/epidemiologia
Queratinas Específicas do Cabelo/genética
Mutação de Sentido Incorreto
[Mh] Termos MeSH secundário: Alopecia/etnologia
Alopecia/genética
Análise Mutacional de DNA
Éxons/genética
Feminino
Genes Recessivos
Cabelo/anormalidades
Doenças do Cabelo
Folículo Piloso/patologia
Haplótipos/genética
Seres Humanos
Masculino
Linhagem
Fenótipo
Federação Russa
Amostragem
Deleção de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Keratins, Hair-Specific)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160224
[St] Status:MEDLINE


  7 / 163 MEDLINE  
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[PMID]:26855150
[Au] Autor:Noll EM; Eisen C; Stenzinger A; Espinet E; Muckenhuber A; Klein C; Vogel V; Klaus B; Nadler W; Rösli C; Lutz C; Kulke M; Engelhardt J; Zickgraf FM; Espinosa O; Schlesner M; Jiang X; Kopp-Schneider A; Neuhaus P; Bahra M; Sinn BV; Eils R; Giese NA; Hackert T; Strobel O; Werner J; Büchler MW; Weichert W; Trumpp A; Sprick MR
[Ad] Endereço:Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM), Heidelberg, Germany.
[Ti] Título:CYP3A5 mediates basal and acquired therapy resistance in different subtypes of pancreatic ductal adenocarcinoma.
[So] Source:Nat Med;22(3):278-87, 2016 Mar.
[Is] ISSN:1546-170X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Although subtypes of pancreatic ductal adenocarcinoma (PDAC) have been described, this malignancy is clinically still treated as a single disease. Here we present patient-derived models representing the full spectrum of previously identified quasi-mesenchymal (QM-PDA), classical and exocrine-like PDAC subtypes, and identify two markers--HNF1A and KRT81--that enable stratification of tumors into different subtypes by using immunohistochemistry. Individuals with tumors of these subtypes showed substantial differences in overall survival, and their tumors differed in drug sensitivity, with the exocrine-like subtype being resistant to tyrosine kinase inhibitors and paclitaxel. Cytochrome P450 3A5 (CYP3A5) metabolizes these compounds in tumors of the exocrine-like subtype, and pharmacological or short hairpin RNA (shRNA)-mediated CYP3A5 inhibition sensitizes tumor cells to these drugs. Whereas hepatocyte nuclear factor 4, alpha (HNF4A) controls basal expression of CYP3A5, drug-induced CYP3A5 upregulation is mediated by the nuclear receptor NR1I2. CYP3A5 also contributes to acquired drug resistance in QM-PDA and classical PDAC, and it is highly expressed in several additional malignancies. These findings designate CYP3A5 as a predictor of therapy response and as a tumor cell-autonomous detoxification mechanism that must be overcome to prevent drug resistance.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/metabolismo
Carcinoma Ductal Pancreático/genética
Citocromo P-450 CYP3A/genética
Resistência a Medicamentos Antineoplásicos/genética
Regulação Neoplásica da Expressão Gênica
Fator 1-alfa Nuclear de Hepatócito/metabolismo
Queratinas Específicas do Cabelo/metabolismo
Queratinas Tipo II/metabolismo
Neoplasias Pancreáticas/genética
[Mh] Termos MeSH secundário: Idoso
Animais
Carcinoma Ductal Pancreático/tratamento farmacológico
Carcinoma Ductal Pancreático/metabolismo
Dasatinibe/uso terapêutico
Cloridrato de Erlotinib/uso terapêutico
Feminino
Fator 4 Nuclear de Hepatócito/metabolismo
Seres Humanos
Imuno-Histoquímica
Masculino
Camundongos Endogâmicos NOD
Meia-Idade
Transplante de Neoplasias
Paclitaxel/uso terapêutico
Neoplasias Pancreáticas/tratamento farmacológico
Neoplasias Pancreáticas/metabolismo
Prognóstico
Inibidores de Proteínas Quinases/uso terapêutico
Receptores de Esteroides/metabolismo
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (HNF1A protein, human); 0 (HNF4A protein, human); 0 (Hepatocyte Nuclear Factor 1-alpha); 0 (Hepatocyte Nuclear Factor 4); 0 (KRT81 protein, human); 0 (Keratins, Hair-Specific); 0 (Keratins, Type II); 0 (Protein Kinase Inhibitors); 0 (Receptors, Steroid); 0 (pregnane X receptor); DA87705X9K (Erlotinib Hydrochloride); EC 1.14.14.1 (CYP3A5 protein, human); EC 1.14.14.1 (Cytochrome P-450 CYP3A); P88XT4IS4D (Paclitaxel); RBZ1571X5H (Dasatinib)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160209
[St] Status:MEDLINE
[do] DOI:10.1038/nm.4038


  8 / 163 MEDLINE  
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[PMID]:26778557
[Au] Autor:Mashukova A; Forteza R; Salas PJ
[Ad] Endereço:Department of Physiology, Nova Southeastern University, Ft. Lauderdale, Florida, USA; Department of Cell Biology, University of Miami Miller School of Medicine, Miami, Florida, USA.
[Ti] Título:Functional Analysis of Keratin-Associated Proteins in Intestinal Epithelia: Heat-Shock Protein Chaperoning and Kinase Rescue.
[So] Source:Methods Enzymol;569:139-54, 2016.
[Is] ISSN:1557-7988
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A growing body of evidence from several laboratories points at nonmechanical functions of keratin intermediate filaments (IF), such as control of apoptosis, modulation of signaling, or regulation of innate immunity, among others. While these functions are generally assigned to the ability of IF to scaffold other proteins, direct mechanistic causal relationships between filamentous keratins and the observed effects of keratin knockout or mutations are still missing. We have proposed that the scaffolding of chaperones such as Hsp70/40 may be key to understand some IF nonmechanical functions if unique features or specificity of the chaperoning activity in the IF scaffold can be demonstrated. The same criteria of uniqueness could be applied to other biochemical functions of the IF scaffold. Here, we describe a subcellular fractionation technique based on established methods of keratin purification. The resulting keratin-enriched fraction contains several proteins tightly associated with the IF scaffold, including Hsp70/40 chaperones. Being nondenaturing, this fractionation method enables direct testing of chaperoning and other enzymatic activities associated with IF, as well as supplementation experiments to determine the need for soluble (cytosolic) proteins. This method also permits to analyze inhibitory activity of cytosolic proteins at independently characterized physiological concentrations. When used as complementary approaches to knockout, knockdown, or site-directed mutagenesis, these techniques are expected to shed light on molecular mechanisms involved in the effects of IF loss of function.
[Mh] Termos MeSH primário: Proteínas de Choque Térmico/química
Queratinas Específicas do Cabelo/química
Proteína Quinase C/química
[Mh] Termos MeSH secundário: Animais
Células CACO-2
Fracionamento Celular
Seres Humanos
Filamentos Intermediários/enzimologia
Mucosa Intestinal/citologia
Camundongos
Fosforilação
Dobramento de Proteína
Processamento de Proteína Pós-Traducional
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Heat-Shock Proteins); 0 (Keratins, Hair-Specific); EC 2.7.11.13 (PKC-3 protein); EC 2.7.11.13 (Protein Kinase C)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160119
[St] Status:MEDLINE


  9 / 163 MEDLINE  
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[PMID]:26756110
[Au] Autor:Volkov V; Cavaco-Paulo A
[Ad] Endereço:Centro de Engenharia Biológica (CEB), Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
[Ti] Título:Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties.
[So] Source:Int J Biol Macromol;85:476-86, 2016 Apr.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The current study describes the in vitro phosphorylation of a human hair keratin, using protein kinase for the first time. Phosphorylation of keratin was demonstrated by (31)P NMR (Nuclear Magnetic Resonance) and Diffuse Reflectance Infrared Fourier Transform (DRIFT) techniques. Phosphorylation induced a 2.5 fold increase of adsorption capacity in the first 10 min for cationic moiety like methylene blue (MB). Thorough description of MB adsorption process was performed by several isothermal models. Reconstructed fluorescent microscopy images depict distinct amounts of dye bound to the differently treated hair. The results of this work suggest that the enzymatic phosphorylation of keratins might have significant implications in hair shampooing and conditioning, where short application times of cationic components are of prime importance.
[Mh] Termos MeSH primário: Cátions/química
Queratinas Específicas do Cabelo/química
[Mh] Termos MeSH secundário: Adsorção
Seres Humanos
Concentração de Íons de Hidrogênio
Cinética
Azul de Metileno/química
Fosforilação
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cations); 0 (Keratins, Hair-Specific); T42P99266K (Methylene Blue)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160113
[St] Status:MEDLINE


  10 / 163 MEDLINE  
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[PMID]:26578733
[Au] Autor:Robles AI; Ryan BM
[Ad] Endereço:Laboratory of Human Carcinogenesis, NCI-CCR, National Institutes of Health, Bethesda, USA.
[Ti] Título:KRT81 miR-SNP rs3660 is associated with risk and survival of NSCLC.
[So] Source:Ann Oncol;27(2):360-1, 2016 Feb.
[Is] ISSN:1569-8041
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Carcinoma Pulmonar de Células não Pequenas/genética
Queratinas Específicas do Cabelo/genética
Queratinas Tipo II/genética
Neoplasias Pulmonares/genética
MicroRNAs/genética
Polimorfismo de Nucleotídeo Único
[Mh] Termos MeSH secundário: Feminino
Seres Humanos
Masculino
[Pt] Tipo de publicação:COMMENT; LETTER; RESEARCH SUPPORT, N.I.H., INTRAMURAL
[Nm] Nome de substância:
0 (Keratins, Hair-Specific); 0 (Keratins, Type II); 0 (MicroRNAs)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:170201
[Lr] Data última revisão:
170201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151119
[St] Status:MEDLINE
[do] DOI:10.1093/annonc/mdv552



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