Base de dados : MEDLINE
Pesquisa : D05.750.078.730.475.470 [Categoria DeCS]
Referências encontradas : 348 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 35 ir para página                         

  1 / 348 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29259037
[Au] Autor:Falch CM; Sundaram AYM; Øystese KA; Normann KR; Lekva T; Silamikelis I; Eieland AK; Andersen M; Bollerslev J; Olarescu NC
[Ad] Endereço:Section of Specialized EndocrinologyDepartment of Endocrinology cafal14@student.sdu.dk.
[Ti] Título:Gene expression profiling of fast- and slow-growing non-functioning gonadotroph pituitary adenomas.
[So] Source:Eur J Endocrinol;178(3):295-307, 2018 Mar.
[Is] ISSN:1479-683X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Reliable biomarkers associated with aggressiveness of non-functioning gonadotroph adenomas (GAs) are lacking. As the growth of tumor remnants is highly variable, molecular markers for growth potential prediction are necessary. We hypothesized that fast- and slow-growing GAs present different gene expression profiles and reliable biomarkers for tumor growth potential could be identified, focusing on the specific role of epithelial-mesenchymal transition (EMT). DESIGN AND METHODS: Eight GAs selected for RNA sequencing were equally divided into fast- and slow-growing group by the tumor volume doubling time (TVDT) median (27.75 months). Data were analyzed by tophat2, cufflinks and cummeRbund pipeline. 40 genes were selected for RT-qPCR validation in 20 GAs based on significance, fold-change and pathway analyses. The effect of silencing (metadherin) and (endomucin) on migration of human adenoma cells was evaluated. RESULTS: 350 genes were significantly differentially expressed (282 genes upregulated and 68 downregulated in the fast group, -adjusted <0.05). Among 40 selected genes, 11 showed associations with TVDT (-0.669< <-0.46, < 0.05). These were and six EMT-related genes ( and ). , but not , demonstrated involvement in cell migration and association with EMT markers. CONCLUSIONS: Fast- and slow-growing GAs present different gene expression profiles, and genes related to EMT have higher expression in fast-growing tumors. In addition to , identified as an important contributor to aggressiveness, the other genes might represent markers for tumor growth potential and possible targets for drug therapy.
[Mh] Termos MeSH primário: Adenoma/genética
Transição Epitelial-Mesenquimal/genética
Neoplasias Hipofisárias/genética
RNA Mensageiro/metabolismo
[Mh] Termos MeSH secundário: Proteínas Adaptadoras de Transdução de Sinal/genética
Adenoma/metabolismo
Adulto
Idoso
Caderinas/genética
Moléculas de Adesão Celular/genética
Movimento Celular/genética
Subunidades Catalíticas da Proteína Quinase Dependente de AMP Cíclico/genética
Feminino
Hormônio Foliculoestimulante/metabolismo
Perfilação da Expressão Gênica
Regulação Neoplásica da Expressão Gênica
Inativação Gênica
Seres Humanos
Técnicas In Vitro
Peptídeos e Proteínas de Sinalização Intracelular/genética
Hormônio Luteinizante/metabolismo
Masculino
Glicoproteínas de Membrana/genética
Proteínas Associadas aos Microtúbulos/genética
Meia-Idade
Miosina Tipo I/genética
Proteínas do Tecido Nervoso/genética
Neoplasias Hipofisárias/metabolismo
Proteínas Proto-Oncogênicas/genética
Receptores de Superfície Celular/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Ribonucleases/genética
Sialoglicoproteínas/genética
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adaptor Proteins, Signal Transducing); 0 (Cadherins); 0 (Cell Adhesion Molecules); 0 (EMCN protein, human); 0 (GPM6A protein, human); 0 (Intracellular Signaling Peptides and Proteins); 0 (MTDH protein, human); 0 (MYO1B protein, human); 0 (Membrane Glycoproteins); 0 (Microtubule-Associated Proteins); 0 (Nerve Tissue Proteins); 0 (PCDH18 protein, human); 0 (Proto-Oncogene Proteins); 0 (RNA, Messenger); 0 (Receptors, Cell Surface); 0 (SKIL protein, human); 0 (SPAG9 protein, human); 0 (Sialoglycoproteins); 0 (UNC5H4 protein, human); 0 (hook1 protein, human); 9002-67-9 (Luteinizing Hormone); 9002-68-0 (Follicle Stimulating Hormone); EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinase Catalytic Subunits); EC 2.7.11.11 (PRKACB protein, human); EC 3.1.- (CNOT6L protein, human); EC 3.1.- (Ribonucleases); EC 3.6.1.- (Myosin Type I)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1530/EJE-17-0702


  2 / 348 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29235966
[Au] Autor:Myronovskij SL; Boiko NM; Chumak VV; Shorobura MS; Lootsyk MD; Stoika RS; Kit YY
[Ti] Título:The characteristics of antibodies of mice immunized by human unconventional myosin 1c.
[So] Source:Ukr Biochem J;88(6):63-9, 2016 Nov-Dec.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:Specific antibodies produced against a protein of interest are invaluable tools for monitoring the protein structure, intracellular location and biological activity. Inoculation of murine lymphoma cells into the peritoneal cavity of immunized mice provides generation of ascitic fluid containing a significant amount of antibody with desired antigen specificity. Here we demonstrated that the intraperitoneal administration of murine lymphoma NK/Ly cells in mice immunized with 48 kDa isoform of human blood serum unconventional myosin 1c leads to generation of ascitic fluid that contained specific IgG-antibodies. These antibodies were capable of binding of the unconventional myosin 1c isolated from blood serum of patients with multiple sclerosis, rheumatoid arthritis and systemic lupus erythematosis, and could be used for diagnostics of several autoimmune diseases, the multiple sclerosis in particular.
[Mh] Termos MeSH primário: Antígenos/administração & dosagem
Líquido Ascítico/imunologia
Imunoglobulina G/isolamento & purificação
Linfoma/imunologia
Miosina Tipo I/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Artrite Reumatoide/sangue
Artrite Reumatoide/diagnóstico
Líquido Ascítico/química
Feminino
Seres Humanos
Imunização
Imunoglobulina G/biossíntese
Imunoglobulina G/química
Injeções Intraperitoneais
Lúpus Eritematoso Sistêmico/sangue
Lúpus Eritematoso Sistêmico/diagnóstico
Linfoma/patologia
Camundongos
Camundongos Endogâmicos BALB C
Esclerose Múltipla/sangue
Esclerose Múltipla/diagnóstico
Transplante de Neoplasias
Células Tumorais Cultivadas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens); 0 (Immunoglobulin G); EC 3.6.1.- (Myosin Type I); EC 3.6.1.3 (MYO1C protein, human)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.06.063


  3 / 348 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29173820
[Au] Autor:Encinar Del Dedo J; Idrissi FZ; Fernandez-Golbano IM; Garcia P; Rebollo E; Krzyzanowski MK; Grötsch H; Geli MI
[Ad] Endereço:Institute for Molecular Biology of Barcelona (CSIC), Baldiri Reixac 15, 08028 Barcelona, Spain.
[Ti] Título:ORP-Mediated ER Contact with Endocytic Sites Facilitates Actin Polymerization.
[So] Source:Dev Cell;43(5):588-602.e6, 2017 Dec 04.
[Is] ISSN:1878-1551
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Oxysterol binding protein-related proteins (ORPs) are conserved lipid binding polypeptides, enriched at ER contacts sites. ORPs promote non-vesicular lipid transport and work as lipid sensors in the context of many cellular tasks, but the determinants of their distinct localization and function are not understood. Here, we demonstrate that the yeast endocytic invaginations associate with the ER and that this association specifically requires the ORPs Osh2 and Osh3, which bridge the endocytic myosin-I Myo5 to the ER integral-membrane VAMP-associated protein (VAP) Scs2. Disruption of the ER contact with endocytic sites using ORP, VAP, myosin-I, or reticulon mutants delays and weakens actin polymerization and interferes with vesicle scission. Finally, we provide evidence suggesting that ORP-dependent sterol transfer facilitates actin polymerization at endocytic sites.
[Mh] Termos MeSH primário: Actinas/metabolismo
Retículo Endoplasmático/metabolismo
Metabolismo dos Lipídeos/fisiologia
[Mh] Termos MeSH secundário: Animais
Transporte Biológico
Miosina Tipo I/metabolismo
Receptores de Esteroides/metabolismo
Saccharomyces cerevisiae/metabolismo
Esteróis/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins); 0 (Receptors, Steroid); 0 (Sterols); 0 (oxysterol binding protein); EC 3.6.1.- (Myosin Type I)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


  4 / 348 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28893906
[Au] Autor:Zattelman L; Regev R; Usaj M; Reinke PYA; Giese S; Samson AO; Taft MH; Manstein DJ; Henn A
[Ad] Endereço:From the Faculty of Biology, Technion-Israel Institute of Technology, Haifa 3200003, Israel.
[Ti] Título:N-terminal splicing extensions of the human gene fine-tune the kinetics of the three full-length myosin IC isoforms.
[So] Source:J Biol Chem;292(43):17804-17818, 2017 Oct 27.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The gene produces three alternatively spliced isoforms, differing only in their N-terminal regions (NTRs). These isoforms, which exhibit both specific and overlapping nuclear and cytoplasmic functions, have different expression levels and nuclear-cytoplasmic partitioning. To investigate the effect of NTR extensions on the enzymatic behavior of individual isoforms, we overexpressed and purified the three full-length human isoforms from suspension-adapted HEK cells. MYO1C favored the actomyosin closed state (AM ), MYO1C populated the actomyosin open state (AM ) and AM equally, and MYO1C favored the AM state. Moreover, the full-length constructs isomerized before ADP release, which has not been observed previously in truncated MYO1C constructs. Furthermore, global numerical simulation analysis predicted that MYO1C populated the actomyosin·ADP closed state (AMD ) 5-fold more than the actomyosin·ADP open state (AMD ) and to a greater degree than MYO1C and MYO1C (4- and 2-fold, respectively). On the basis of a homology model of the 35-amino acid NTR of MYO1C (NTR ) docked to the X-ray structure of MYO1C , we predicted that MYO1C NTR residue Arg-21 would engage in a specific interaction with post-relay helix residue Glu-469, which affects the mechanics of the myosin power stroke. In addition, we found that adding the NTR peptide to MYO1C yielded a protein that transiently mimics MYO1C kinetic behavior. By contrast, NTR , which harbors the R21G mutation, was unable to confer MYO1C -like kinetic behavior. Thus, the NTRs affect the specific nucleotide-binding properties of MYO1C isoforms, adding to their kinetic diversity. We propose that this level of fine-tuning within MYO1C broadens its adaptability within cells.
[Mh] Termos MeSH primário: Processamento Alternativo
Miosina Tipo I
[Mh] Termos MeSH secundário: Actomiosina/química
Actomiosina/genética
Actomiosina/metabolismo
Difosfato de Adenosina/química
Difosfato de Adenosina/genética
Difosfato de Adenosina/metabolismo
Substituição de Aminoácidos
Cristalografia por Raios X
Células HEK293
Seres Humanos
Isoenzimas
Mutação de Sentido Incorreto
Miosina Tipo I/química
Miosina Tipo I/genética
Miosina Tipo I/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Isoenzymes); 61D2G4IYVH (Adenosine Diphosphate); 9013-26-7 (Actomyosin); EC 3.6.1.- (Myosin Type I); EC 3.6.1.3 (MYO1C protein, human)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.794008


  5 / 348 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28777082
[Au] Autor:Gautam G; Rehman SAA; Pandey P; Gourinath S
[Ad] Endereço:School of Life Sciences, Jawaharlal Nehru University, New Delhi, Delhi 110 067, India.
[Ti] Título:Crystal structure of the PEG-bound SH3 domain of myosin IB from Entamoeba histolytica reveals its mode of ligand recognition.
[So] Source:Acta Crystallogr D Struct Biol;73(Pt 8):672-682, 2017 Aug 01.
[Is] ISSN:2059-7983
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The versatility in the recognition of various interacting proteins by the SH3 domain drives a variety of cellular functions. Here, the crystal structure of the C-terminal SH3 domain of myosin IB from Entamoeba histolytica (EhMySH3) is reported at a resolution of 1.7 Šin native and PEG-bound states. Comparisons with other structures indicated that the PEG molecules occupy protein-protein interaction pockets similar to those occupied by the peptides in other peptide-bound SH3-domain structures. Also, analysis of the PEG-bound EhMySH3 structure led to the recognition of two additional pockets, apart from the conventional polyproline and specificity pockets, that are important for ligand interaction. Molecular-docking studies combined with various comparisons revealed structural similarity between EhMySH3 and the SH3 domain of ß-Pix, and this similarity led to the prediction that EhMySH3 preferentially binds targets containing type II-like PXXP motifs. These studies expand the understanding of the EhMySH3 domain and provide extensive structural knowledge, which is expected to help in predicting the interacting partners which function together with myosin IB during phagocytosis in E. histolytica infections.
[Mh] Termos MeSH primário: Entamoeba histolytica/metabolismo
Miosina Tipo I/metabolismo
Polietilenoglicóis/metabolismo
Proteínas de Protozoários/metabolismo
Domínios de Homologia de src
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Cristalografia por Raios X
Entamoeba histolytica/química
Entamebíase/parasitologia
Seres Humanos
Ligantes
Simulação de Acoplamento Molecular
Miosina Tipo I/química
Polietilenoglicóis/química
Ligação Proteica
Multimerização Proteica
Proteínas de Protozoários/química
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ligands); 0 (Protozoan Proteins); 30IQX730WE (Polyethylene Glycols); EC 3.6.1.- (Myosin Type I)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170805
[St] Status:MEDLINE
[do] DOI:10.1107/S2059798317009639


  6 / 348 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Mucha, José Nelson
Vieira, Alexandre Rezende
Texto completo
[PMID]:28364893
[Au] Autor:Cruz CV; Mattos CT; Maia JC; Granjeiro JM; Reis MF; Mucha JN; Vilella B; Ruellas AC; Luiz RR; Costa MC; Vieira AR
[Ad] Endereço:Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.
[Ti] Título:Genetic polymorphisms underlying the skeletal Class III phenotype.
[So] Source:Am J Orthod Dentofacial Orthop;151(4):700-707, 2017 Apr.
[Is] ISSN:1097-6752
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Our goal was to verify the association between candidate polymorphisms and skeletal Class III malocclusion in a well-characterized homogeneous sample set. METHODS: Thirty-five single-nucleotide polymorphisms were studied from 10 candidate loci in 54 Class III subjects and 120 controls. Skeletal Class III characteristics included ANB angle less than 0°, SNB angle greater than 83° (mandibular prognathism), SNA angle less than 79° (maxillary deficiency), Class III molar relationship, and negative overjet. Inclusion criteria for the controls were ANB angle between 0° and 4°, Class I molar relationship, and normal overjet. Chi-square and Fisher exact tests and principal component (PC) analysis were used to determine overrepresentation of marker alleles with alpha of 0.05. Odds ratios and 95% confidence intervals were calculated. RESULTS: MYO1H (rs10850110 AG) (P = 0.001) with PC2 and between FGF10 (rs593307 A
[Mh] Termos MeSH primário: Má Oclusão de Angle Classe III/genética
Polimorfismo de Nucleotídeo Único/genética
[Mh] Termos MeSH secundário: Proteínas de Transporte/genética
Proteínas de Transporte/fisiologia
Estudos de Casos e Controles
Feminino
Fator 10 de Crescimento de Fibroblastos/genética
Fator 10 de Crescimento de Fibroblastos/fisiologia
Estudos de Associação Genética
Seres Humanos
Masculino
Miosina Tipo I/genética
Miosina Tipo I/fisiologia
Polimorfismo de Nucleotídeo Único/fisiologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (FGF10 protein, human); 0 (Fibroblast Growth Factor 10); 0 (myosin 1H, human); 0 (somatotropin-binding protein); EC 3.6.1.- (Myosin Type I)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170425
[Lr] Data última revisão:
170425
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:170403
[St] Status:MEDLINE


  7 / 348 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28214148
[Au] Autor:Stufft K; Elgin J; Patterson B; Matarneh SK; Preisser R; Shi H; England EM; Scheffler TL; Mills EW; Gerrard DE
[Ad] Endereço:Department of Animal and Poultry Sciences, Litton-Reaves Hall, Virginia Tech, Blacksburg, VA 24061, USA.
[Ti] Título:Muscle characteristics only partially explain color variations in fresh hams.
[So] Source:Meat Sci;128:88-96, 2017 Jun.
[Is] ISSN:1873-4138
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Fresh hams display significant lean color variation that persists through further processing and contributes to a less desirable cured product. In an attempt to understand the underlying cause of this color disparity, we evaluated the differences in muscle characteristics and energy metabolites across semimembranosus (SM) muscles differing in color variation. The L* (lightness) and a* (redness) values were highest and lowest (P<0.001), respectfully in the most caudal aspects of the muscle while the ultimate pH was the lowest (P<0.001). Correspondingly, this region possessed highest (P<0.01) glycolytic potential (GP) and lactate dehydrogenase (LDH) levels but did not differ in the amount of myoglobin or myosin heavy chain type I isoform. These data show that differences in muscle may contribute to ham color variation but suggest other factors may mitigate or exacerbate these variances.
[Mh] Termos MeSH primário: Qualidade dos Alimentos
Glicólise
Músculos Isquiotibiais/metabolismo
L-Lactato Desidrogenase/metabolismo
Carne/análise
Pigmentos Biológicos/análise
[Mh] Termos MeSH secundário: Animais
Alimentos em Conserva/análise
Músculos Isquiotibiais/enzimologia
Músculos Isquiotibiais/crescimento & desenvolvimento
Concentração de Íons de Hidrogênio
Mioglobina/metabolismo
Cadeias Pesadas de Miosina/metabolismo
Miosina Tipo I/metabolismo
Pigmentos Biológicos/metabolismo
Reprodutibilidade dos Testes
Sus scrofa
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Myoglobin); 0 (Pigments, Biological); EC 1.1.1.27 (L-Lactate Dehydrogenase); EC 3.6.1.- (Myosin Type I); EC 3.6.4.1 (Myosin Heavy Chains)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170219
[St] Status:MEDLINE


  8 / 348 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28088345
[Au] Autor:Meguro S; Akamatsu T; Matsushima S; Kosugi I; Kawasaki H; Arai Y; Baba S; Tsuchida T; Shido Y; Suda T; Iwashita T
[Ad] Endereço:Department of Regenerative and Infectious Pathology, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka 431-3192, Japan.
[Ti] Título:Phenotypic characterization of perivascular myoid cell neoplasms, using myosin 1B, a newly identified human pericyte marker.
[So] Source:Hum Pathol;62:187-198, 2017 Apr.
[Is] ISSN:1532-8392
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Our aims were to identify pericyte-specific markers for the analysis of formalin-fixed, paraffin-embedded human tissue samples, and to characterize perivascular myoid cell neoplasms phenotypically. Previously identified pericyte markers failed to distinguish pericytes from other cellular types, such as vascular smooth muscle cells (vSMCs) and fibroblasts, in immunohistochemistry analysis. However, we compared gene expression profiles between pericytes, vSMCs, and fibroblasts, and performed human skin vasculature immunohistochemistry analysis, which led to the identification of myosin 1B (MYO1B) as a novel pericyte marker. Afterward, we investigated the expression levels of MYO1B and h-caldesmon (h-CD) in perivascular myoid cell neoplasms, angioleiomyomas (n=28), glomus tumors (n=23), and myopericytomas (n=3). Angioleiomyomas were shown to contain MYO1B-negative and h-CD-positive (MYO1B hCD ) tumor cells, with vSMC features. Glomus tumors were predominantly composed of the MYO1B hCD tumor cells, with the intermediate features between pericytes and vSMCs, whereas MYO1B hCD tumor cells with pericytic features and/or the MYO1B hCD tumor cells with vSMC features were frequently found in these tumors. The perivascular concentric pattern of 2 myopericytoma cases was composed of MYO1B hCD tumor cells, whereas that of one myopericytoma contained MYO1B hCD tumor cells. These results indicate that the ability to distinguish between these cell types may allow us to understand the differentiation and origin of perivascular myoid cell neoplasms. This is the first study to identify cell properties of perivascular myoid cell neoplasms by using a pericyte-specific marker with considerably lower expression in vSMCs and fibroblasts.
[Mh] Termos MeSH primário: Angiomioma/química
Biomarcadores Tumorais/análise
Tumor Glômico/química
Miofibroma/química
Miosina Tipo I/análise
Pericitos/química
Neoplasias de Tecidos Moles/química
[Mh] Termos MeSH secundário: Adulto
Idoso
Angiomioma/genética
Angiomioma/patologia
Animais
Biomarcadores Tumorais/genética
Biópsia
Proteínas de Ligação a Calmodulina/análise
Diferenciação Celular
Linhagem da Célula
Feminino
Fibroblastos/química
Imunofluorescência
Perfilação da Expressão Gênica
Tumor Glômico/genética
Tumor Glômico/patologia
Seres Humanos
Masculino
Camundongos Endogâmicos C57BL
Camundongos Transgênicos
Meia-Idade
Miócitos de Músculo Liso/química
Miócitos de Músculo Liso/patologia
Miofibroma/genética
Miofibroma/patologia
Miosina Tipo I/genética
Pericitos/patologia
Fenótipo
Pele/química
Neoplasias de Tecidos Moles/genética
Neoplasias de Tecidos Moles/patologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (Calmodulin-Binding Proteins); 0 (MYO1B protein, human); EC 3.6.1.- (Myosin Type I)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170424
[Lr] Data última revisão:
170424
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170116
[St] Status:MEDLINE


  9 / 348 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27914536
[Au] Autor:Wollenberg RD; Donau SS; Nielsen TT; Sørensen JL; Giese H; Wimmer R; Søndergaard TE
[Ad] Endereço:Department of Chemistry and Bioscience, Aalborg University, DK-9220 Aalborg, Denmark. Electronic address: rwo@bio.aau.dk.
[Ti] Título:Real-time imaging of the growth-inhibitory effect of JS399-19 on Fusarium.
[So] Source:Pestic Biochem Physiol;134:24-30, 2016 Nov.
[Is] ISSN:1095-9939
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Real-time imaging was used to study the effects of a novel Fusarium-specific cyanoacrylate fungicide (JS399-19) on growth and morphology of four Fusarium sp. This fungicide targets the motor domain of type I myosin. Fusarium graminearum PH-1, Fusarium solani f. sp. pisi 77-13-4, Fusarium avenaceum IBT8464, and Fusarium avenaceum 05001, which has a K216Q amino-acid substitution at the resistance-implicated site in its myosin type I motor domain, were analyzed. Real-time imaging shows that JS399-19 inhibits fungal growth but not to the extent previously reported. The fungicide causes the hypha to become entangled and unable to extend vertically. This implies that type I myosin in Fusarium is essential for hyphal and mycelia propagation. The K216Q substitution correlates with reduced susceptibility in F. avenaceum.
[Mh] Termos MeSH primário: Aminoácidos/farmacologia
Fungicidas Industriais/farmacologia
Fusarium/efeitos dos fármacos
Fenilpropionatos/farmacologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Farmacorresistência Fúngica/genética
Proteínas Fúngicas/química
Fusarium/citologia
Fusarium/genética
Fusarium/crescimento & desenvolvimento
Hifas/efeitos dos fármacos
Hifas/crescimento & desenvolvimento
Microscopia
Miosina Tipo I/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-cyano-3-amino-3-phenylacrylic acid); 0 (Amino Acids); 0 (Fungal Proteins); 0 (Fungicides, Industrial); 0 (Phenylpropionates); EC 3.6.1.- (Myosin Type I)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170313
[Lr] Data última revisão:
170313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161205
[St] Status:MEDLINE


  10 / 348 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27759032
[Au] Autor:Patton J; Brewer C; Chien W; Johnston JJ; Griffith AJ; Biesecker LG
[Ad] Endereço:Medical Genomics and Metabolic Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.
[Ti] Título:A genotypic ascertainment approach to refute the association of MYO1A variants with non-syndromic deafness.
[So] Source:Eur J Hum Genet;25(1):147-149, 2016 Jan.
[Is] ISSN:1476-5438
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Variants in the unconventional myosin gene, MYO1A, have been reported to cause non-syndromic sensorineural hearing loss with a pattern of autosomal dominant inheritance. Others have challenged this association. We used a genotypic ascertainment study design to test the association of MYO1A variants with hearing loss. We evaluated MYO1A variants from a cohort of 951 individuals with exome sequencing who were not ascertained for hearing loss. Five individuals had one of two variants claimed to be associated with sensorineural hearing loss in a prior study and 33 individuals had one of 13 predicted deleterious variants. We obtained audiology evaluations for 12 individuals with these variants of interest. The hearing acuity of the participants was compared with age- and sex-matched controls and published age- and sex-specific reference ranges from a large population of otologically screened adults. None of the participants had bilateral sensorineural hearing loss of moderate or greater severity. These data do not support a causal relationship of variants in MYO1A to sensorineural hearing loss. We suggest that the genotypic ascertainment method is useful to objectively evaluate gene-phenotype associations.
[Mh] Termos MeSH primário: Predisposição Genética para Doença
Perda Auditiva Neurossensorial/genética
Perda Auditiva/genética
Cadeias Pesadas de Miosina/genética
Miosina Tipo I/genética
[Mh] Termos MeSH secundário: Idoso
Exoma/genética
Feminino
Estudos de Associação Genética
Genótipo
Perda Auditiva/fisiopatologia
Perda Auditiva Neurossensorial/fisiopatologia
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Masculino
Meia-Idade
Linhagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MYO1A protein, human); EC 3.6.1.- (Myosin Type I); EC 3.6.4.1 (Myosin Heavy Chains)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161021
[St] Status:MEDLINE
[do] DOI:10.1038/ejhg.2016.140



página 1 de 35 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde