Base de dados : MEDLINE
Pesquisa : D05.750.078.875.750 [Categoria DeCS]
Referências encontradas : 273 [refinar]
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  1 / 273 MEDLINE  
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[PMID]:27771849
[Au] Autor:Lapphanichayakool P; Sutheerawattananonda M; Limpeanchob N
[Ad] Endereço:Department of Pharmacy Practice, Faculty of Pharmaceutical Science, Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok, 65000, Thailand.
[Ti] Título:Hypocholesterolemic effect of sericin-derived oligopeptides in high-cholesterol fed rats.
[So] Source:J Nat Med;71(1):208-215, 2017 Jan.
[Is] ISSN:1861-0293
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The beneficial effect of cholesterol-lowering proteins and/or peptides derived from various dietary sources is continuously reported. A non-dietary protein from silk cocoon, sericin, has also demonstrated cholesterol-lowering activity. A sericin hydrolysate prepared by enzymatic hydrolysis was also expected to posses this effect. The present study was aimed at investigating the cholesterol-lowering effect of sericin peptides, so called "sericin-derived oligopeptides" (SDO) both in vivo and in vitro. The results showed that SDO at all three doses tested (10 mg kg day , 50 mg kg day , and 200 mg kg day ) suppressed serum total and non-HDL cholesterol levels in rats fed a high-cholesterol diet. Triglyceride and HDL-cholesterol levels were not significantly changed among all groups. The fecal contents of bile acids and cholesterol did not differ among high-cholesterol fed rats. SDO dose-dependently reduced cholesterol solubility in lipid micelles, and inhibited cholesterol uptake in monolayer Caco-2 cells. SDO also effectively bound to all three types of bile salts including taurocholate, deoxytaurocholate, and glycodeoxycholate. Direct interaction with bile acids of SDO may disrupt micellar cholesterol solubility, and subsequently reduce the absorption of dietary cholesterol in intestines. Taking all data together, SDO or sericin peptides exhibit a beneficial effect on blood cholesterol levels and could be potentially used as a health-promoting dietary supplement or nutraceutical product.
[Mh] Termos MeSH primário: Colesterol/sangue
Hipercolesterolemia/tratamento farmacológico
Sericinas/uso terapêutico
Triglicerídeos/metabolismo
[Mh] Termos MeSH secundário: Animais
Células CACO-2
Modelos Animais de Doenças
Seres Humanos
Masculino
Oligopeptídeos
Ratos
Ratos Sprague-Dawley
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oligopeptides); 0 (Sericins); 0 (Triglycerides); 97C5T2UQ7J (Cholesterol)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1007/s11418-016-1050-9


  2 / 273 MEDLINE  
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[PMID]:29381729
[Au] Autor:Qian P; Jiang T; Wang X; Song F; Chen C; Shen X
[Ad] Endereço:Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, China.
[Ti] Título:bmo-miR-275 down-regulates expression of Bombyx mori sericin gene 2 in vitro.
[So] Source:PLoS One;13(1):e0190464, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We hypothesized that bmo-miR-275 has a potential regulatory function regarding the expression of sericin gene 2 (BmSer-2). First, we examined the expression of bmo-miR-275 and its target gene BmSer-2 in seven different tissues from 5th instar day-3 silkworm larvae. The results showed that they were both specifically expressed in the middle silk gland, implying that spatio-temporal conditions are required for bmo-miR-275 to regulate the expression of BmSer-2. To test this hypothesis, we constructed a pri-bmo-miR-275 expressing plasmid pcDNA3.0 [ie1-egfp-pri-bmo-miR-275-SV40] and BmSer-2-3´UTR recombinant reporter plasmids pGL3.0 [A3-luc-Ser-2-3' UTR-SV40]. Finally, BmN cells were harvested and luciferase activity was detected. Results showed that luciferase activity was reduced significantly (P<0.05) in BmN cells co-transfected with pcDNA3.0 [ie1-egfp-pri-bmo-miR-275-SV40] and pGL3.0 [A3-luc-Ser-2-3'UTR-SV40], suggesting that bmo-miR-275 can down-regulate the expression of BmSer-2 in vitro. Our results improve the understanding of the regulatory function of Bombyx mori miRNA on the expression of genes regulating silk formation.
[Mh] Termos MeSH primário: Bombyx/genética
Regulação para Baixo
Genes de Insetos
Sericinas/genética
[Mh] Termos MeSH secundário: Regiões 3' não Traduzidas
Animais
Bombyx/crescimento & desenvolvimento
Linhagem Celular
Técnicas In Vitro
Larva/metabolismo
MicroRNAs/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (3' Untranslated Regions); 0 (MicroRNAs); 0 (Sericins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180131
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190464


  3 / 273 MEDLINE  
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[PMID]:28684113
[Au] Autor:Ampawong S; Isarangkul D; Aramwit P
[Ad] Endereço:Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, Ratchawithi Road, Ratchathewi, Bangkok 10400, Thailand. Electronic address: am_sumate@hotmail.com.
[Ti] Título:Sericin improves heart and liver mitochondrial architecture in hypercholesterolaemic rats and maintains pancreatic and adrenal cell biosynthesis.
[So] Source:Exp Cell Res;358(2):301-314, 2017 Sep 15.
[Is] ISSN:1090-2422
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hypercholesterolaemia is well known to be associated with mitochondrial dysfunction, subsequently leading to multiple organ failure. Similar to other natural products, sericin is a candidate for adjunctive therapy in hyperlipidaemic conditions. However, the cholesterol-lowering mechanisms of sericin are multifactorial and controversial. Here, a high-cholesterol-fed rat model with or without sericin treatment was established using a dosage of 1000mg/kg/day for 30 days. Blood lipid profiles, oxidative stress markers (superoxide dismutase, SOD; malondialdehyde, MDA; nuclear factor erythroid 2-related factor, Nrf-2), dysmorphic mitochondria in relation to fission (dynamin-related protein-1; Drp-1) and fusion (guanosine triphosphatase mutated in dominant optic atrophy; OPA-1) markers and biosynthetic markers (aquaporin, AQP-1; tubulin-4ß, Tb4B) in the pancreas and adrenal gland were evaluated. The results showed that sericin reduced blood cholesterol and increased high-density lipoprotein (HDL) by acting against oxidative stress. Hypocholesterolaemic and antioxidant conditions further preserved heart and liver mitochondrial architecture; however, this protection was not exhibited in the kidney, where a high level of renal mitophagy, indicating by LC-3 up-regulation, was presented. The steps of ultrastructural alteration of mitochondria from degenerative changes to necrosis were also demonstrated. Sericin also conserved AQP-1 and Tb4B levels in the exocrine pancreatic acinar cells and zona glomerulosa cells, which were positively correlated with serum lipase, HDL, antioxidative markers and mitochondrial integrity. The present study revealed that sericin not only has antioxidant capacity but also balances pancreatic and adrenal cell biosynthesis, especially lipase activity, which may have played an important role in improving lipid dysregulation in the hypercholesterolaemic rat model, leading to the reduction of dysmorphic mitochondria, particularly in the heart and liver.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Coração/efeitos dos fármacos
Hipercolesterolemia/tratamento farmacológico
Fígado/efeitos dos fármacos
Mitocôndrias/efeitos dos fármacos
Sericinas/farmacologia
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Colesterol/metabolismo
Coração/fisiopatologia
Fígado/metabolismo
Mitocôndrias/metabolismo
Estresse Oxidativo/efeitos dos fármacos
Pâncreas/metabolismo
Ratos Sprague-Dawley
Triglicerídeos/metabolismo
Regulação para Cima/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Sericins); 0 (Triglycerides); 97C5T2UQ7J (Cholesterol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170708
[St] Status:MEDLINE


  4 / 273 MEDLINE  
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[PMID]:28381799
[Au] Autor:Nagai N; Ogata F; Deguchi S; Ueno A; Kawasaki N; Ito Y
[Ad] Endereço:Faculty of Pharmacy, Kindai University.
[Ti] Título:Combination Ointment Containing Solid Tranilast Nanoparticles and Dissolved Sericin Is Efficacious for Treating Skin Wound-Healing Deficits and Redness in Diabetic Rats.
[So] Source:Biol Pharm Bull;40(4):444-450, 2017.
[Is] ISSN:1347-5215
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:We attempted to design a combination ointment containing solid tranilast nanoparticles and dissolved sericin as a wound-healing drug (TS-combination ointment), and evaluated its usefulness as therapy for wound-healing deficits in streptozotocin-induced diabetic rat (STZ rat) using kinetic analyses as an index. Solid tranilast nanoparticles were prepared by bead mill methods with low-substituted methylcellulose; the mean particle size of the tranilast nanoparticles was 70 nm. The ointment was designed to contain the tranilast nanoparticles plus sericin powder and/or Carbopol 934. Skin wound healing in STZ rats begins significantly later than in normal rats. Although the skin wound healing rate in STZ rats treated with an ointment containing tranilast nanoparticles was lower than in STZ rats treated with vehicle, the ointment was effective in reducing redness. An ointment containing sericin enhanced the skin-healing rate, but the preventive effect on redness was weak. On the other hand, the combination of tranilast and sericin increased both the skin healing rate and reduction in redness. In conclusion, we have adapted kinetic analyses to skin wound healing in rats, and found these analyses to be useful as an index of wound healing ability by a wound-healing drug. In addition, we show that treatment with the TS-combination ointment enhances the skin wound healing rate and reduces redness. These findings provide information significant to the search for new wound-healing therapies and for the design of wound-healing drugs.
[Mh] Termos MeSH primário: Anti-Inflamatórios não Esteroides/administração & dosagem
Diabetes Mellitus Experimental/tratamento farmacológico
Nanopartículas/administração & dosagem
Sericinas/administração & dosagem
Cicatrização/efeitos dos fármacos
ortoaminobenzoatos/administração & dosagem
[Mh] Termos MeSH secundário: Administração Tópica
Animais
Diabetes Mellitus Experimental/patologia
Quimioterapia Combinada
Masculino
Pomadas
Ratos
Ratos Wistar
Resultado do Tratamento
Cicatrização/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Ointments); 0 (Sericins); 0 (ortho-Aminobenzoates); HVF50SMY6E (tranilast)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170724
[Lr] Data última revisão:
170724
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE
[do] DOI:10.1248/bpb.b16-00812


  5 / 273 MEDLINE  
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[PMID]:28357595
[Au] Autor:Ma S; Xia X; Li Y; Sun L; Liu Y; Liu Y; Wang X; Shi R; Chang J; Zhao P; Xia Q
[Ad] Endereço:State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, People's Republic of China.
[Ti] Título:Increasing the yield of middle silk gland expression system through transgenic knock-down of endogenous sericin-1.
[So] Source:Mol Genet Genomics;292(4):823-831, 2017 Aug.
[Is] ISSN:1617-4623
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Various genetically modified bioreactor systems have been developed to meet the increasing demands of recombinant proteins. Silk gland of Bombyx mori holds great potential to be a cost-effective bioreactor for commercial-scale production of recombinant proteins. However, the actual yields of proteins obtained from the current silk gland expression systems are too low for the proteins to be dissolved and purified in a large scale. Here, we proposed a strategy that reducing endogenous sericin proteins would increase the expression yield of foreign proteins. Using transgenic RNA interference, we successfully reduced the expression of BmSer1 to 50%. A total 26 transgenic lines expressing Discosoma sp. red fluorescent protein (DsRed) in the middle silk gland (MSG) under the control of BmSer1 promoter were established to analyze the expression of recombinant. qRT-PCR and western blotting showed that in BmSer1 knock-down lines, the expression of DsRed had significantly increased both at mRNA and protein levels. We did an additional analysis of DsRed/BmSer1 distribution in cocoon and effect of DsRed protein accumulation on the silk fiber formation process. This study describes not only a novel method to enhance recombinant protein expression in MSG bioreactor, but also a strategy to optimize other bioreactor systems.
[Mh] Termos MeSH primário: Reatores Biológicos
Bombyx/genética
Bombyx/metabolismo
Interferência de RNA
Proteínas Recombinantes/biossíntese
Sericinas/genética
Seda/biossíntese
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Proteínas Luminescentes/biossíntese
Proteínas Luminescentes/genética
Regiões Promotoras Genéticas/genética
RNA Mensageiro/biossíntese
RNA Interferente Pequeno/genética
Reação em Cadeia da Polimerase em Tempo Real
Proteínas Recombinantes/genética
Seda/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Luminescent Proteins); 0 (RNA, Messenger); 0 (RNA, Small Interfering); 0 (Recombinant Proteins); 0 (Sericins); 0 (Silk); 0 (red fluorescent protein)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170803
[Lr] Data última revisão:
170803
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170331
[St] Status:MEDLINE
[do] DOI:10.1007/s00438-017-1311-7


  6 / 273 MEDLINE  
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[PMID]:28237330
[Au] Autor:Hajarian H; Aghaz F; Karami Shabankareh H
[Ad] Endereço:IVF Laboratory, Department of Animal Science, Faculty of Agriculture, Razi University, Kermanshah, Iran. Electronic address: h.hajarian@razi.ac.ir.
[Ti] Título:Replacement of serum with sericin in in vitro maturation and culture media: Effects on embryonic developmental competence of Sanjabi sheep embryo during breeding season.
[So] Source:Theriogenology;92:144-148, 2017 Apr 01.
[Is] ISSN:1879-3231
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sericin is a water-soluble component of silk and has been used as a biomaterial due to its antibacterial and ultraviolet radiation-resistant properties. This study was designed to evaluate the effect of sericin supplementation, as a serum replacement, in maturation and culture media on the meiotic competence of oocytes or in vitro culture of ovine embryos. In experiment 1, oocytes were matured in the presence of 10% fetal ovine serum (FOS), 0.1% polyvinyl alcohol (PVA) and different concentrations of sericin (0.1, 0.5, 1 and 2.5%), for 24 h. The addition of 0.5% sericin to maturation medium increased the rates of maturation to metaphase II of oocytes compared with those in cultures with 0.1% PVA. Following fertilization, blastocyst development was higher for oocytes matured with 0.5% of sericin compared with 0.1% PVA. However, the rates of nuclear maturation of oocytes and blastocyst development under FOS and 0.5% sericin were not significantly different. In experiment 2, presumptive zygotes were cultured in the presence of 10% FOS, 0.1% PVA and different concentrations of sericin (0.1, 0.5, 1 and 2.5%), for 7-8 days. The addition of 0.5% sericin to culture medium increased the blastocyst rate compared with those in cultures without sericin or addition of 0.1% PVA and 10% FOS. These results indicate the feasibility of sericin as an alternative protein supplement for IVM and IVC in ovine oocytes and zygotes.
[Mh] Termos MeSH primário: Meios de Cultura/farmacologia
Técnicas de Cultura Embrionária/veterinária
Embrião de Mamíferos/efeitos dos fármacos
Sericinas/farmacologia
Soro/química
Ovinos/embriologia
[Mh] Termos MeSH secundário: Animais
Meios de Cultura/química
Desenvolvimento Embrionário/efeitos dos fármacos
Desenvolvimento Embrionário/fisiologia
Feminino
Fertilização In Vitro/veterinária
Técnicas de Maturação in Vitro de Oócitos
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (Sericins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170227
[St] Status:MEDLINE


  7 / 273 MEDLINE  
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[PMID]:28183615
[Au] Autor:Rao J; Cheng Y; Liu Y; Ye Z; Zhan B; Quan D; Xu Y
[Ad] Endereço:Department of Arthopedics Surgery, People's Hospital of Quanzhou, Quanzhou, Zhengjiang 324000, People's Republic of China. Electronic address: raojianwei05@sina.com.
[Ti] Título:A multi-walled silk fibroin/silk sericin nerve conduit coated with poly(lactic-co-glycolic acid) sheath for peripheral nerve regeneration.
[So] Source:Mater Sci Eng C Mater Biol Appl;73:319-332, 2017 Apr 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The linearly oriented multi-walled silk fibroin/silk sericin (SF/SS) nerve conduits (NCs) can provide physical cues similar to native peripheral nerve fasciculi, but the mechanical properties of which are not excellent enough. In this study, NCs with a novel and bionic design with dual structures were developed. The important features of our NCs is that the internal skeleton (the multi-walled SF/SS conduits) has a bionic structure similar to the architecture of native peripheral nerve fasciculi, which is beneficial for nerve regeneration, and the outer sheath (the hollow poly(lactic-co-glycolic acid) [PLGA] conduits) could provide strong mechanical protection for the internal skeleton. The linearly oriented multi-walled SF/SS conduit was fabricated and inserted in the hollow PLGA sheath lumen and then used for the bridge across the sciatic nerve defect in rats. The outcome of the peripheral nerve repair post implantation was evaluated. The functional and morphological parameters were examined and showed that the novel PLGA-coated SF/SS NCs could promote peripheral nerve regeneration, approaching those elicited by nerve autografts that are the first candidate for repair of peripheral nerve defects. Thus, these updated NCs have potential usefulness to enhance functional recovery after repair of peripheral nerve defect.
[Mh] Termos MeSH primário: Materiais Revestidos Biocompatíveis/farmacologia
Fibroínas/farmacologia
Regeneração Tecidual Guiada/métodos
Ácido Láctico/química
Regeneração Nervosa/efeitos dos fármacos
Ácido Poliglicólico/química
Nervo Isquiático/fisiologia
Sericinas/farmacologia
[Mh] Termos MeSH secundário: Animais
Bombyx
Força Compressiva
Masculino
Bainha de Mielina/efeitos dos fármacos
Fibras Nervosas/efeitos dos fármacos
Implante de Prótese
Ratos Sprague-Dawley
Nervo Isquiático/efeitos dos fármacos
Nervo Isquiático/patologia
Nervo Isquiático/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coated Materials, Biocompatible); 0 (Sericins); 0 (polylactic acid-polyglycolic acid copolymer); 26009-03-0 (Polyglycolic Acid); 33X04XA5AT (Lactic Acid); 9007-76-5 (Fibroins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170903
[Lr] Data última revisão:
170903
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE


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[PMID]:28181828
[Au] Autor:Scrivano L; Iacopetta D; Sinicropi MS; Saturnino C; Longo P; Parisi OI; Puoci F
[Ad] Endereço:a Department of Pharmacy, Health and Nutritional Sciences, University of Calabria , Rende , CS , Italy.
[Ti] Título:Synthesis of sericin-based conjugates by click chemistry: enhancement of sunitinib bioavailability and cell membrane permeation.
[So] Source:Drug Deliv;24(1):482-490, 2017 Nov.
[Is] ISSN:1521-0464
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Sericin is a natural protein that has been used in biomedical and pharmaceutical fields as raw material for polypeptide-based drug delivery systems (DDSs). In this paper, it has been employed as pharmaceutical biopolymer for the production of sunitinib-polypeptide conjugate. The synthesis has been carried out by simple click reaction in water, using the redox couple l-ascorbic acid/hydrogen peroxide as a free radical grafting initiator. The bioconjugate molecular weight (50 kDa < Mw < 75 kDa) was obtained by SDS-PAGE, while the spectroscopic characteristics have been studied in order to reveal the presence of grafted sunitinib. In both FT-IR and UV/Vis spectra, signals corresponding to sunitinib functional groups have been identified. Since sunitinib is an anticancer drug characterized by low bioavailability and low permeability, the bioconjugation aimed at their enhancement. In vitro studies demonstrated that bioavailability has been increased to almost 74%, compared with commercial formulation. Also cell membrane permeability has been augmented in in vitro tests, in which membrane models have been used to determine the lipid membrane/physiological fluid partition coefficient (Kp). The log(Kp) value of the bioconjugate was increased to over 4. This effect resulted in a three-fold decrease of IC value against MCF-7 cells.
[Mh] Termos MeSH primário: Antineoplásicos/metabolismo
Permeabilidade da Membrana Celular
Química Click
Portadores de Fármacos
Indóis/metabolismo
Inibidores de Proteínas Quinases/metabolismo
Pirróis/metabolismo
Sericinas/síntese química
Neoplasias do Colo do Útero/tratamento farmacológico
[Mh] Termos MeSH secundário: Antineoplásicos/administração & dosagem
Antineoplásicos/química
Disponibilidade Biológica
Sobrevivência Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Composição de Medicamentos
Feminino
Células HeLa
Seres Humanos
Indóis/administração & dosagem
Indóis/química
Concentração Inibidora 50
Inibidores de Proteínas Quinases/administração & dosagem
Inibidores de Proteínas Quinases/química
Pirróis/administração & dosagem
Pirróis/química
Solubilidade
Espectrofotometria Ultravioleta
Espectroscopia de Infravermelho com Transformada de Fourier
Tecnologia Farmacêutica/métodos
Neoplasias do Colo do Útero/metabolismo
Neoplasias do Colo do Útero/patologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Drug Carriers); 0 (Indoles); 0 (Protein Kinase Inhibitors); 0 (Pyrroles); 0 (Sericins); V99T50803M (sunitinib)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170403
[Lr] Data última revisão:
170403
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170210
[St] Status:MEDLINE
[do] DOI:10.1080/10717544.2016.1267822


  9 / 273 MEDLINE  
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[PMID]:27987455
[Au] Autor:Yang Y; Cai Y; Sun N; Li R; Li W; Kundu SC; Kong X; Yao J
[Ad] Endereço:The Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, National Engineering Lab for Textile Fiber Materials and Processing Technology (Zhejiang), College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, China.
[Ti] Título:Biomimetic synthesis of sericin and silica hybrid colloidosomes for stimuli-responsive anti-cancer drug delivery systems.
[So] Source:Colloids Surf B Biointerfaces;151:102-111, 2017 Mar 01.
[Is] ISSN:1873-4367
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Colloidosomes are becoming popular due to their significant flexibility with respect to microcapsule functionality. This study reports a facile approach for synthesizing silica colloidosomes by using sericin microcapsule as the matrix in an environment-friendly method. The silica colloid arrangement on the sericin microcapsules are orchestrated by altering the reaction parameters. Doxorubicin (DOX), used as a hydrophilic anti-cancer drug model, is encapsulated into the colloidosomes in a mild aqueous solution and becomes stimuli-responsive to different external environments, including pH values, protease, and ionic strength are also observed. Colloidosomes with sub-monolayers, close-packed monolayers, and close-packed multi-layered SiO colloid shells can be fabricated under the optimized reaction conditions. A flexible DOX release from colloidosomes can be obtained via modulating the SiO colloid layer arrangement and thickness. The close-packed and multi-layered SiO colloid shells can best protect the colloidosomes and delay the rapid cargo release. MG-63 cells are killed when doxorubicin is released from the microcapsules due to degradation in the microenvironment of cancer cells. The drug release period is prolonged as SiO shell thickness and integrity increase. This work suggests that the hybrid colloidosomes can be effective in a bioactive molecule delivery system.
[Mh] Termos MeSH primário: Antineoplásicos/química
Materiais Biomiméticos/síntese química
Coloides/síntese química
Sistemas de Liberação de Medicamentos
Sericinas/síntese química
Dióxido de Silício/síntese química
[Mh] Termos MeSH secundário: Materiais Biomiméticos/química
Cápsulas
Linhagem Celular Tumoral
Sobrevivência Celular
Coloides/química
Doxorrubicina/química
Liberação Controlada de Fármacos
Seres Humanos
Concentração de Íons de Hidrogênio
Íons
Microscopia Eletrônica de Varredura
Microscopia Eletrônica de Transmissão
Necrose
Concentração Osmolar
Tamanho da Partícula
Peptídeo Hidrolases/química
Sericinas/química
Dióxido de Silício/química
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Capsules); 0 (Colloids); 0 (Ions); 0 (Sericins); 7631-86-9 (Silicon Dioxide); 80168379AG (Doxorubicin); EC 3.4.- (Peptide Hydrolases)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170328
[Lr] Data última revisão:
170328
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161218
[St] Status:MEDLINE


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[PMID]:27903836
[Au] Autor:Ampawong S; Isarangkul D; Aramwit P
[Ad] Endereço:1 Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.
[Ti] Título:Sericin ameliorated dysmorphic mitochondria in high-cholesterol diet/streptozotocin rat by antioxidative property.
[So] Source:Exp Biol Med (Maywood);242(4):411-421, 2017 Feb.
[Is] ISSN:1535-3699
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Sericin has been implicated in lower cholesterolemic effect due to its properties with several mechanisms. Mitochondria are one of the most important targets to be affected in high blood cholesterol and glucose conditions. The protective role of sericin on mitochondria remains doubtful. To examine this role, electron microscopic, histopathologic, immunohistochemical, and biochemical studies were performed in a high-cholesterol diet/streptozotocin rat model. The results demonstrated that sericin reduced blood cholesterol without hypoglycemic effect. Sericin alleviated dysmorphic mitochondria in heart and liver but not in kidney and also decreased peculiar endoplasmic reticulum in the exocrine pancreas. In addition, sericin decreased hepatic steatosis and preserved zymogen granule referable to the decline of reactive oxygen species production in hepatic mitochondrial extraction and down-regulation of malondialdehyde expression in the liver and exocrine pancreas however irrelevant to lipase activity. This study suggests that sericin has antioxidative property to reduce blood cholesterol by means of diminishing fat deposit in hepatocyte and improves mitochondria and endoplasmic reticulum integrities. [Box: see text].
[Mh] Termos MeSH primário: Antioxidantes/uso terapêutico
Glicemia/efeitos dos fármacos
Diabetes Mellitus Experimental/tratamento farmacológico
Fígado Gorduroso/tratamento farmacológico
Hipercolesterolemia/tratamento farmacológico
Mitocôndrias/metabolismo
Sericinas/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Bombyx
Colesterol/sangue
Dieta Hiperlipídica
Retículo Endoplasmático/metabolismo
Feminino
Rim/metabolismo
Rim/ultraestrutura
Fígado/metabolismo
Fígado/ultraestrutura
Malondialdeído/metabolismo
Miocárdio/metabolismo
Miocárdio/ultraestrutura
Estresse Oxidativo/efeitos dos fármacos
Pâncreas/metabolismo
Pâncreas/ultraestrutura
Ratos
Ratos Wistar
Espécies Reativas de Oxigênio/metabolismo
Estreptozocina/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Blood Glucose); 0 (Reactive Oxygen Species); 0 (Sericins); 4Y8F71G49Q (Malondialdehyde); 5W494URQ81 (Streptozocin); 97C5T2UQ7J (Cholesterol)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170801
[Lr] Data última revisão:
170801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161202
[St] Status:MEDLINE
[do] DOI:10.1177/1535370216681553



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