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[PMID]:29231025
[Au] Autor:Shen X; Li M; Wang YL; Chen YL; Lin Y; Zhao ZM; Que TZ
[Ad] Endereço:Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China.
[Ti] Título:[Comparison of MPure-12 Automatic Nucleic Acid Purification and Chelex-100 Method].
[So] Source:Fa Yi Xue Za Zhi;33(2):168-170, 2017 Apr.
[Is] ISSN:1004-5619
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVES: To explore the forensic application value of MPure-12 automatic nucleic acid purification (MPure-12 Method) for DNA extraction by extracting and typing DNA from bloodstains and various kinds of biological samples with different DNA contents. METHODS: Nine types of biological samples, such as bloodstains, semen stains, and saliva were collected. DNA were extracted using MPure-12 method and Chelex-100 method, followed by PCR amplification and electrophoresis for obtaining STR-profiles. RESULTS: The samples such as hair root, chutty, butt, muscular tissue, saliva stain, bloodstain and semen stain were typed successfully by MPure-12 method. Partial alleles were lacked in the samples of saliva, and the genotyping of contact swabs was unsatisfactory. Additional, all of the bloodstains (20 µL, 15 µL, 10 µL, 5 µL, 1 µL) showed good typing results using Chelex-100 method. But the loss of alleles occurred in 1 µL blood volume by MPure-12 method. CONCLUSIONS: MPure-12 method is suitable for DNA extraction of a certain concentration blood samples.Chelex-100 method may be better for the extraction of trace blood samples.This instrument used in nucleic acid extraction has the advantages of simplicity of operator, rapidity, high extraction efficiency, high rate of reportable STR-profiles and lower man-made pollution.
[Mh] Termos MeSH primário: Quelantes
DNA/isolamento & purificação
Medicina Legal/métodos
Reação em Cadeia da Polimerase/métodos
Poliestirenos
Polivinil
[Mh] Termos MeSH secundário: Alelos
Manchas de Sangue
DNA/sangue
Impressões Digitais de DNA
Genótipo
Seres Humanos
Masculino
Resinas Sintéticas
Saliva
Sêmen/química
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chelating Agents); 0 (Polystyrenes); 0 (Polyvinyls); 0 (Resins, Synthetic); 11139-85-8 (Chelex 100); 80208-96-4 (chelex); 9007-49-2 (DNA)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.3969/j.issn.1004-5619.2017.02.013


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[PMID]:29241957
[Au] Autor:Luo YD; Zhang QL; Yao SJ; Lin DQ
[Ad] Endereço:Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China.
[Ti] Título:Evaluation of adsorption selectivity of immunoglobulins M, A and G and purification of immunoglobulin M with mixed-mode resins.
[So] Source:J Chromatogr A;1533:77-86, 2018 Jan 19.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This study investigated adsorption selectivity of immunoglobulin M (IgM), immunoglobulin A (IgA) and immunoglobulin (IgG) on four mixed-mode resins with the functional ligands of 4-mercatoethyl-pyridine (MEP), 2-mercapto-1-methylimidazole (MMI), 5-aminobenzimidazole (ABI) and tryptophan-5-aminobenzimidazole (W-ABI), respectively. IgM purification processes with mixed-mode resins were also proposed. All resins showed typical pH-dependent adsorption, and high adsorption capacity was found at pH 5.0-8.0 with low adsorption capacity under acidic conditions. Meanwhile, high selectivity of IgM/IgA and IgM/IgG was obtained with ABI-4FF and MMI-4FF resins at pH 4.0-5.0, which was used to develop a method for IgM, IgA and IgG separation by controlling loading and elution pH. Capture of monoclonal IgM from cell culture supernatant with ABI-4FF resins was studied and high purity (∼99%) and good recovery (80.8%) were obtained. Moreover, IgM direct separation from human serum with combined two-step chromatography (ABI-4FF and MMI-4FF) was investigated, and IgM purity of 65.2% and a purification factor of 28.3 were obtained after optimization. The antibody activity of IgM was maintained after purification. The results demonstrated that mixed-mode chromatography with specially-designed ligands is a promising way to improve adsorption selectivity and process efficiency of IgM purification from complex feedstock.
[Mh] Termos MeSH primário: Técnicas de Química Analítica/métodos
Cromatografia
Imunoglobulina M/isolamento & purificação
Resinas Sintéticas/química
[Mh] Termos MeSH secundário: Adsorção
Células Cultivadas
Técnicas de Química Analítica/normas
Seres Humanos
Interações Hidrofóbicas e Hidrofílicas
Imunoglobulina A/metabolismo
Imunoglobulina G/metabolismo
Imunoglobulina M/metabolismo
Ligantes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoglobulin A); 0 (Immunoglobulin G); 0 (Immunoglobulin M); 0 (Ligands); 0 (Resins, Synthetic)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE


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[PMID]:29185324
[Au] Autor:Winter S
[Ti] Título:Treating Post-Orthodontic White Spots: A Conservative Resin Infiltration Technique.
[So] Source:Dent Today;35(10):122-3, 2016 Oct.
[Is] ISSN:8750-2186
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Cárie Dentária/etiologia
Cárie Dentária/terapia
Braquetes Ortodônticos/efeitos adversos
Resinas Sintéticas/administração & dosagem
[Mh] Termos MeSH secundário: Adulto
Tratamento Conservador
Feminino
Seres Humanos
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Resins, Synthetic); 0 (icon infiltrant)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE


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[PMID]:29327892
[Au] Autor:Potran M; Strbac B; Puskar T; Hadzistevic; Hodolic J; Trifkovic B
[Ti] Título:Measurement of the accuracy of dental working casts using a coordinate measuring machine.
[So] Source:Vojnosanit Pregl;73(10):895-903, 2016 Oct.
[Is] ISSN:0042-8450
[Cp] País de publicação:Serbia
[La] Idioma:eng
[Ab] Resumo:Background/Aim: Dental impressions present a negative imprint of intraoral tissues of a patient which is, by pouring in gypsum, transferred extraorally on the working cast. Casting an accurate and precise working cast presents the first and very important step, since each of the following stages contributes to the overall error of the production process, which can lead to inadequately fitting dental restorations. The aim of this study was to promote and test a new model and technique for in vitro evaluation of the dental impression accuracy, as well as to asses the dimensional stability of impression material depending on the material bulk, and its effect on the accuracy of working casts. Methods: Impressions were made by the monophasic technique using the experimental master model. Custom trays with spacing of 1, 2 and 3 mm were constructed by rapid prototyping. The overall of 10 impressions were made with each custom tray. Working casts were made with gypsum type IV. Measurement of working casts was done 24 h later using a co-ordinate measuring machine. Results: The obtained results show that the working casts of all the three custom trays were in most cases significantly different in the transversal and sagittal planes in relation to the master model. The height of abutments was mainly unaffected. The degree of convergence showed certain significance in all the three custom trays, most pronounced in the tray with 3 mm spacing. Conclusion: The impression material bulk of 1­3 mm could provide accurate working casts when using the monophasic impression technique. The increase of the distance between abutment teeth influences the accuracy of working casts depending on the material bulk. [Projekat Ministarstva nauke Republike Srbije, br. TR 35020: Research and development of modelling methods and approaches in manufacturing of dental recoveries with the application of modern technologies and computer aided systems]
[Mh] Termos MeSH primário: Sulfato de Cálcio/normas
Técnica de Fundição Odontológica/normas
Materiais para Moldagem Odontológica/normas
Técnica de Moldagem Odontológica/normas
Modelos Dentários/normas
Bases de Dentadura/normas
Planejamento de Dentadura/normas
[Mh] Termos MeSH secundário: Sulfato de Cálcio/química
Projeto Auxiliado por Computador
Materiais para Moldagem Odontológica/química
Seres Humanos
Reprodutibilidade dos Testes
Resinas Sintéticas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dental Impression Materials); 0 (Resins, Synthetic); WAT0DDB505 (Calcium Sulfate)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE
[do] DOI:10.2298/VSP150105089P


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[PMID]:29235338
[Au] Autor:Simos S
[Ti] Título:Three Impression Material Classifications: A Comparison.
[So] Source:Dent Today;36(3):76,78, 80-1, 2017 Mar.
[Is] ISSN:8750-2186
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Materiais para Moldagem Odontológica/classificação
Técnica de Moldagem Odontológica
[Mh] Termos MeSH secundário: Implantação Dentária Endo-Óssea/métodos
Implantes Dentários
Prótese Dentária Fixada por Implante
Restauração Dentária Permanente/métodos
Estética Dentária
Feminino
Seres Humanos
Masculino
Polivinil
Resinas Sintéticas
Siloxanas
[Pt] Tipo de publicação:CASE REPORTS; COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dental Implants); 0 (Dental Impression Materials); 0 (Impregum); 0 (Polyvinyls); 0 (Resins, Synthetic); 0 (Siloxanes); 0 (vinyl polysiloxane)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE


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[PMID]:27777071
[Au] Autor:Kong K; Hiraishi N; Nassar M; Otsuki M; Yiu CKY; Tagami J
[Ad] Endereço:Cariology and Operative Dentistry, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan; General Dentistry, Roomchang Dental and Aesthetic Hospital, Phnom Penh, Cambodia.
[Ti] Título:Effect of phytic acid etchant on resin-dentin bonding: Monomer penetration and stability of dentin collagen.
[So] Source:J Prosthodont Res;61(3):251-258, 2017 Jul.
[Is] ISSN:2212-4632
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Phytic acid (IP6) works well as an etchant in dentin bonding to remove the smear layer due to its acidity and chelating effect. This study compared the etching effect of IP6 with phosphoric acid (PA) and ethylenediaminetetraacetic acid (EDTA) on resin-dentin bond strength, micromorphology of the etched dentin surface and nanoleakage formation along resin-dentin interfaces and compared the protecting effect against collagen degradation. METHODS: Dentin disks and flat dentin surfaces were obtained from extracted human teeth. Specimens were etched with 35% PA (15s), 0.5M EDTA (30s) or 1% IP6 (30s). The surfaces and longitudinal sections of the etched dentin disks were observed using field emission scanning electron microscope (FE-SEM). An etch-and-rinse adhesive was used to create composite build up-specimens for microtensile bond strength (µTBS) testing and nanoleakage observation. To evaluate the effect on collagen degradation, demineralized bovine root dentin blocks were challenged with bacterial collagenase and then observed under light microscope. RESULTS: PA- and EDTA- treated groups showed significantly lower µTBS when compared to IP6-treated group. PA showed distinct nanoleakage and severe collagen degradation. Only slight nanoleakage was detected in IP6 group. IP6 showed better effect than EDTA in preventing collagen degradation induced by bacterial collagenase. CONCLUSIONS: IP6 effectively removed the smear layer and etched dentin, providing high bond strength values and causing minimal nanoleakage and slight collagen degradation.
[Mh] Termos MeSH primário: Ataque Ácido Dentário
Colágeno
Colagem Dentária
Corrosão Dentária
Dentina
Ácido Fítico
Resinas Sintéticas
[Mh] Termos MeSH secundário: Ácido Edético
Seres Humanos
Ácidos Fosfóricos
Proteólise
Resistência à Tração
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phosphoric Acids); 0 (Resins, Synthetic); 7IGF0S7R8I (Phytic Acid); 9007-34-5 (Collagen); 9G34HU7RV0 (Edetic Acid); E4GA8884NN (phosphoric acid)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


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[PMID]:28934372
[Au] Autor:Minoura K; Yamada M; Mizoguchi T; Kaneko T; Nishiyama K; Ozminskyj M; Koshizuka T; Wada I; Suzutani T
[Ad] Endereço:LCD Technology Development Center, Development Group, Display Device Company, Sharp Corporation, Tenri, Nara, Japan.
[Ti] Título:Antibacterial effects of the artificial surface of nanoimprinted moth-eye film.
[So] Source:PLoS One;12(9):e0185366, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The antibacterial effect of a nanostructured film, known as "moth-eye film," was investigated. The moth-eye film has artificially formed nano-pillars, consisting of hydrophilic resin with urethane acrylate and polyethylene glycol (PEG) derivatives, all over its surface that replicates a moth's eye. Experiments were performed to compare the moth-eye film with a flat-surfaced film produced from the same materials. The JIS Z2801 film-covering method revealed that the two films produced a decrease in Staphylococcus aureus and Esherichia coli titers of over 5 and 3 logs, respectively. There was no marked difference in the antibacterial effects of the two surfaces. However, the antibacterial effects were reduced by immersion of the films in water. These results indicated that a soluble component(s) of the resin possessed the antibacterial activity, and this component was identified as PEG derivatives by time-of-flight secondary ion mass spectrometry (TOF-SIMS) and Fourier transform infrared spectroscopy (FT-IR). When a small volume of bacterial suspension was dropped on the films as an airborne droplet model, both films showed antibacterial effects, but that of the moth-eye film was more potent. It was considered that the moth-eye structure allowed the bacteria-loaded droplet to spread and allow greater contact between the bacteria and the film surface, resulting in strong adherence of the bacteria to the film and synergistically enhanced bactericidal activity with chemical components. The antibacterial effect of the moth-eye film has been thus confirmed under a bacterial droplet model, and it appears attractive due to its antibacterial ability, which is considered to result not only from its chemical make-up but also from physical adherence.
[Mh] Termos MeSH primário: Antibacterianos/química
Antibacterianos/farmacologia
Nanoestruturas/química
Nanotecnologia
[Mh] Termos MeSH secundário: Escherichia coli/efeitos dos fármacos
Resinas Sintéticas/química
Staphylococcus aureus/efeitos dos fármacos
Molhabilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Resins, Synthetic)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170922
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185366


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Amore, Ricardo
Texto completo SciELO Brasil
[PMID]:28877284
[Au] Autor:Anauate-Netto C; Borelli L; Amore R; DI Hipólito V; D'Alpino PHP
[Ad] Endereço:Universidade Metropolitana de Santos, Centro de Pós-Graduação, Santos, SP, Brasil.
[Ti] Título:Caries progression in non-cavitated fissures after infiltrant application: a 3-year follow-up of a randomized controlled clinical trial.
[So] Source:J Appl Oral Sci;25(4):442-454, 2017 Jul-Aug.
[Is] ISSN:1678-7765
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Objectives: To evaluate the efficacy of a conservative treatment to prevent the progression of caries using an infiltrant on non-cavitated pit and fissures. Material and Methods: This controlled clinical trial selected 23 volunteers with clinically and radiographically non-cavitated occlusal caries among patients presenting a "rather low" to "very high" caries risk. Eighty-six teeth were randomly divided into two experimental groups: teeth receiving a commercial pit-and-fissure sealant (Alpha Seal-DFL) and contralateral teeth receiving Icon infiltrant (DMG). Caries progression was monitored by clinical (laser fluorescence caries detection) and radiographic examination at 12-month intervals over a period of 3 years of monitored caries progression. Probing the sealing materials to detect areas of retention was also used to evaluate marginal integrity. Results: Statistical analysis showed no difference in caries progression using laser fluorescence caries detection when both materials were compared, regardless of the evaluation times (p>0.05). No significance was observed when the marginal sealant integrity of both materials was compared, regardless of the evaluation time (p<0.05). Marginal integrity significantly reduced after 1 year for both materials (p<0.05), but remained stable after 2 and 3 years of evaluation, compared with 1-year results (p>0.05). SEM analysis exhibited a more homogeneous sealing for the infiltrant than obtained by the sealant. Conclusions: The infiltrant was effective to prevent the caries progression in non-cavitated pit-and-fissures after 3 years of clinical evaluation, comparable with the conventional sealant. The infiltrant also presented better results in terms of caries progression at the 3-year evaluation time using the radiographic analysis.
[Mh] Termos MeSH primário: Cárie Dentária/prevenção & controle
Selantes de Fossas e Fissuras/uso terapêutico
Resinas Sintéticas/uso terapêutico
[Mh] Termos MeSH secundário: Adolescente
Criança
Cárie Dentária/diagnóstico por imagem
Progressão da Doença
Feminino
Fluorescência
Seguimentos
Seres Humanos
Masculino
Microscopia Eletrônica de Varredura
Radiografia Dentária
Reprodutibilidade dos Testes
Medição de Risco
Fatores de Risco
Estatísticas não Paramétricas
Propriedades de Superfície
Fatores de Tempo
Resultado do Tratamento
Adulto Jovem
[Pt] Tipo de publicação:CONTROLLED CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pit and Fissure Sealants); 0 (Resins, Synthetic); 0 (icon infiltrant)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


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[PMID]:28872982
[Au] Autor:Cagetti MG; Cattaneo S; Hu YQ; Campus G
[Ti] Título:Amelogenesis Imperfecta: A Non-Invasive Approach to Improve Esthetics in Young Patients. Report of Two Cases.
[So] Source:J Clin Pediatr Dent;41(5):332-335, 2017.
[Is] ISSN:1053-4628
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Objective-Evaluate esthetic and functional efficacy of infiltrant resin (Icon, DMG, Hamburg, Germany) in Amelogenesis Imperfecta's treatment. STUDY DESIGN: Two adolescent patients, G.S. (13 years old) and C.M. (15 years old), affected by the hypomaturation type of Amelogenesis Imperfecta, were treated with Icon resin and were followed for twelve months. RESULTS: Treated teeth show an excellent aesthetical result immediately after the resin application, effect that lasts in the long-term (six and twelve months follow-up examinations); the dental wear's progression seems to be clinically arrested. CONCLUSIONS: Resin infiltration has proven to be a minimal invasive treatment for dental discoloration, less aggressive than conventional procedures. This approach might be recommended for a stable esthetical improvement in moderate AI's lesions especially in children and adolescents.
[Mh] Termos MeSH primário: Amelogênese Imperfeita/terapia
Estética Dentária
Resinas Sintéticas
Clareamento Dental
[Mh] Termos MeSH secundário: Adolescente
Feminino
Seres Humanos
Masculino
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Resins, Synthetic)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:170906
[St] Status:MEDLINE
[do] DOI:10.17796/1053-4628-41.5.332


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[PMID]:28759777
[Au] Autor:Biancalana RC; Freitas Vincenti SA; Alves da Silva RH; Carvalho Panzeri Pires-de-Souza F
[Ad] Endereço:Department of Stomatology, Public Oral Health, and Forensic Dentistry, Ribeirão Preto School of Dentistry - University of São Paulo, Brazil. Electronic address: roberto_cesar2005@yahoo.com.br.
[Ti] Título:Color stability of dental restorative materials submitted to cold temperatures for forensic purposes.
[So] Source:J Forensic Leg Med;51:63-68, 2017 Oct.
[Is] ISSN:1878-7487
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In the post-mortem examination of the dental arches of accident victims in cold locations, dental restorative materials can be found. Cold temperatures can be capable of causing color changes of aesthetic materials, such as composite resin (CR) and glass ionomer cement (GIC). The aim of this study was to evaluate the effect of the cold action on the color stability of CR and GIC restorations, in order to discriminate them and enable the adequate comparison between antemortem and post-mortem data. Sixty bovine teeth (30 CR and 30 GIC) were prepared (6 × 6 × 2 mm) and separated into groups (n = 10). The color readouts were taken by a portable spectrophotometer, before and after of cold action (2.5 °C, -20 °C, -80 °C) inside of freezers. There were color alterations in the coordinates (ΔE, ΔL , Δa e Δb ) for both materials. The authors concluded that cold was capable of producing changes in color in the two esthetic materials, with similar intensities between the two, at all the temperatures studied, when analyzed at 7 days. After being submitted to cold for 30 days, the changes were more significant for CR, allowing it to be differentiated from GIC after 30 days, at all the temperatures tested. Therefore, the test proposed in the study was shown to be practical, feasible and capable of helping Forensic Odontology with the identification of victims.
[Mh] Termos MeSH primário: Temperatura Baixa
Cor
Cimentos de Ionômeros de Vidro
Resinas Sintéticas
Manejo de Espécimes
[Mh] Termos MeSH secundário: Animais
Bovinos
Odontologia Legal
Seres Humanos
Espectrofotometria
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glass Ionomer Cements); 0 (Resins, Synthetic)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE



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