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[PMID]: 29442030 [Au] Autor: Resende AFC; Pereira AF; Moreira TP; Patrício PSO; Fialho SL; Cunha GMF; Silva-Cunha A; Magalhães JT; Silva GR [Ti] Título: PLGA Implants containing vancomycin and dexamethasone: development, characterization and bactericidal effects. [So] Source: Pharmazie;71(8):439-446, 2016 08 01. [Is] ISSN: 0031-7144 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: Post-operative endophthalmitis is an infection and an inflammation of the eye following a surgical procedure. Its treatment is based on drug injections into the eye. However, this treatment can lead to ocular complications. Intraocular implants could substitute the conventional therapy. Poly(lactic-co-glycolic acid) (PLGA) implants comprising on vancomycin and dexamethasone were evaluated as drug delivery system to treat endophthalmitis after cataract surgery. Implants were characterized by drug content uniformity, Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), Wide Angle X-ray Scattering (WAXS), Scanning Electron Microscopy (SEM) and in vitro drug release. The bactericidal effect of vancomycin, eluted from the implants, was demonstrated against Staphylococcus aureus and Staphylococcus epidermidis. The drugs were uniformly distributed in the polymer. The analytical techniques revealed the chemical integrity of the drugs incorporated into the polymer and the modification of dexamethasone semi-crystalline nature. Drugs were controlled released from implants; and the eluted vancomycin showed bactericidal effects. In conclusion, PLGA implants containing vancomycin and dexamethasone may represent a therapeutic alternative to treat post-operative endophthalmitis. [Mh] Termos MeSH primário: Antibacterianos/administração & dosagem Antibacterianos/uso terapêutico Anti-Inflamatórios/administração & dosagem Anti-Inflamatórios/uso terapêutico Bactérias/efeitos dos fármacos Dexametasona/administração & dosagem Dexametasona/uso terapêutico Portadores de Fármacos Ácido Láctico Ácido Poliglicólico Infecção da Ferida Cirúrgica/prevenção & controle Vancomicina/administração & dosagem Vancomicina/uso terapêutico [Mh] Termos MeSH secundário: Antibacterianos/farmacologia Implantes de Medicamento Liberação Controlada de Fármacos Endoftalmite/microbiologia Endoftalmite/prevenção & controle Seres Humanos Testes de Sensibilidade Microbiana Procedimentos Cirúrgicos Oftalmológicos Staphylococcus aureus/efeitos dos fármacos Staphylococcus epidermidis/efeitos dos fármacos Vancomicina/farmacologia [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (Anti-Inflammatory Agents); 0 (Drug Carriers); 0 (Drug Implants); 0 (polylactic acid-polyglycolic acid copolymer); 26009-03-0 (Polyglycolic Acid); 33X04XA5AT (Lactic Acid); 6Q205EH1VU (Vancomycin); 7S5I7G3JQL (Dexamethasone) [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180307 [Lr] Data última revisão: 180307 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180215 [St] Status: MEDLINE [do] DOI: 10.1691/ph.2016.6009

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[PMID]: 29184402 [Au] Autor: Wu X; Wang L; Qiu Y; Zhang B; Hu Z; Jin R [Ad] Endereço: Department of Pediatrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan. [Ti] Título: Cooperation of IRAK1/4 inhibitor and ABT-737 in nanoparticles for synergistic therapy of T cell acute lymphoblastic leukemia. [So] Source: Int J Nanomedicine;12:8025-8034, 2017. [Is] ISSN: 1178-2013 [Cp] País de publicação: New Zealand [La] Idioma: eng [Ab] Resumo: T cell acute lymphoblastic leukemia (T-ALL) is caused by clonal expansion of variant T cell progenitors and is considered as a high risk leukemia. Contemporary single chemotherapy has a limited effect due to dynamic and versatile properties of T-ALL. Here IRAK1/4 inhibitor and ABT-737 were co-encapsulated into polyethylene glycol modified poly (lactic-co-glycolic acid) nanoparticles (IRAK/ABT-NP) to enhance synergistic therapy of T-ALL. The formulation was optimized to achieve high drug loading using Box-Behnken design and response surface methodology. The optimal parameter comprised 2.98% polymer in acetonitrile, a ratio of oil phase to water phase of 1:8.33, and 2.12% emulsifier concentration. High drug loading and uniform spherical shape was achieved. In vitro release study showed sustained release of IRAK1/4 inhibitor for 72 hours as well as sustained release of ABT-737 for more than 120 hours. Uptake efficiency of IRAK/ABT-NP and induced apoptotic T-ALL fraction by IRAK/ABT-NP were much higher than the IRAK1/4 and ABT-737 combined solution. IC of IRAK/ABT-NP was two-fold lower than free drug combination in Jurkat cells. Additionally, we conducted in vivo experiments in which IRAK/ABT-NP exhibited greater cytotoxicity toward T-ALL cells, the capacity to significantly restore white blood cell number in peripheral blood, and improved survival time of T-ALL mouse model compared to the IRAK1/4 and ABT-737 combined solution. [Mh] Termos MeSH primário: Protocolos de Quimioterapia Combinada Antineoplásica/farmacologia Quinases Associadas a Receptores de Interleucina-1/antagonistas & inibidores Nanopartículas/química Leucemia-Linfoma Linfoblástico de Células T Precursoras/tratamento farmacológico [Mh] Termos MeSH secundário: Animais Protocolos de Quimioterapia Combinada Antineoplásica/administração & dosagem Compostos de Bifenilo/administração & dosagem Compostos de Bifenilo/farmacologia Liberação Controlada de Fármacos Sinergismo Farmacológico Feminino Seres Humanos Células Jurkat Ácido Láctico/química Camundongos Nanopartículas/administração & dosagem Nitrofenóis/administração & dosagem Nitrofenóis/farmacologia Piperazinas/administração & dosagem Piperazinas/farmacologia Ácido Poliglicólico/química Sulfonamidas/administração & dosagem Sulfonamidas/farmacologia Ensaios Antitumorais Modelo de Xenoenxerto [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (ABT-737); 0 (Biphenyl Compounds); 0 (Nitrophenols); 0 (Piperazines); 0 (Sulfonamides); 0 (polylactic acid-polyglycolic acid copolymer); 26009-03-0 (Polyglycolic Acid); 33X04XA5AT (Lactic Acid); EC 2.7.11.1 (IRAK1 protein, human); EC 2.7.11.1 (IRAK4 protein, human); EC 2.7.11.1 (Interleukin-1 Receptor-Associated Kinases) [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180305 [Lr] Data última revisão: 180305 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171130 [St] Status: MEDLINE [do] DOI: 10.2147/IJN.S146875

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[PMID]: 29381284 [Au] Autor: Sun F; Shi T; Zhou T; Dong D; Xie J; Wang R; An X; Chen M; Cai J [Ti] Título: 3D Poly(Lactic-co-glycolic acid) Scaffolds for Treating Spinal Cord Injury. [So] Source: J Biomed Nanotechnol;13(3):290-302, 2017 Mar. [Is] ISSN: 1550-7033 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: In this paper, poly(lactic-co-glycolic acid) (PLGA) was used to fabricate spinal cord scaffolds using low temperature deposition manufacturing (LDM) technology. The PLGA scaffolds were characterized as having good porosity, hydrophilicity and considerable biodegradability. The effects of the PLGA scaffolds on cell proliferation and cytotoxicity were evaluated by culturing Schwann cells (SCs) on the surfaces of the scaffolds. The results showed that the SCs spread and proliferated well on the PLGA scaffolds. Histological assessment including Glia fibrillary acidic protein (GFAP) staining, Nissl staining, Luxol fast blue (LFB) staining and Bielschowsky silver staining showed that the spinal cord recoveries considerably improved with the PLGA scaffolds, indicating that the PLGA scaffolds exhibited potential for applications in the management of spinal cord injuries. [Mh] Termos MeSH primário: Regeneração Tecidual Guiada/instrumentação Ácido Láctico/química Ácido Poliglicólico/química Células de Schwann/transplante Traumatismos da Medula Espinal/terapia Regeneração da Medula Espinal/fisiologia Tecidos Suporte [Mh] Termos MeSH secundário: Animais Desenho de Equipamento Análise de Falha de Equipamento Feminino Masculino Impressão Tridimensional Ratos Ratos Sprague-Dawley Células de Schwann/citologia Traumatismos da Medula Espinal/patologia Engenharia Tecidual/instrumentação Resultado do Tratamento [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (polylactic acid-polyglycolic acid copolymer); 26009-03-0 (Polyglycolic Acid); 33X04XA5AT (Lactic Acid) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180223 [Lr] Data última revisão: 180223 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180131 [St] Status: MEDLINE

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[PMID]: 28457961 [Au] Autor: Abu-Awwad HAM; Thiagarajan L; Dixon JE [Ad] Endereço: Wolfson Centre for Stem Cells, Tissue Engineering, and Modelling (STEM), Centre of Biomolecular Sciences, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, UK. [Ti] Título: Controlled release of GAG-binding enhanced transduction (GET) peptides for sustained and highly efficient intracellular delivery. [So] Source: Acta Biomater;57:225-237, 2017 Jul 15. [Is] ISSN: 1878-7568 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Controlled release systems for therapeutic molecules are vital to allow the sustained local delivery of their activities which direct cell behaviour and enable novel regenerative strategies. Direct programming of cells using exogenously delivered transcription factors can by-pass growth factor signalling but there is still a requirement to deliver such activity spatio-temporally. We previously developed a technology termed GAG-binding enhanced transduction (GET) to efficiently deliver a variety of cargoes intracellularly, using GAG-binding domains which promote cell targeting, and cell penetrating peptides (CPPs) which allow cell entry. Herein we demonstrate that GET system can be used in controlled release systems to mediate sustained intracellular transduction over one week. We assessed the stability and activity of GET peptides in poly(dl-lactic acid-co-glycolic acid) (PLGA) microparticles (MPs) prepared using a S/O/W double emulsion method. Efficient encapsulation (∼65%) and tailored protein release profiles could be achieved, however intracellular transduction was significantly inhibited post-release. To retain GET peptide activity we optimized a strategy of co-encapsulation of l-Histidine, which may form a complex with the PLGA degradation products under acidic conditions. Simulations of the polymer microclimate showed that hydrolytic acidic PLGA degradation products directly inhibited GET peptide transduction activity, and use of l-Histidine significantly enhanced released protein delivery. The ability to control the intracellular transduction of functional proteins into cells will facilitate new localized delivery methods and allow approaches to direct cellular behaviour for many regenerative medicine applications. STATEMENT OF SIGNIFICANCE: The goal for regenerative medicine is to restore functional biological tissue by controlling and augmenting cellular behaviour. Either Transcription (TFs) or growth factors (GFs) can be presented to cells in spatio-temporal gradients for programming cell fate and gene expression. Here, we have created a sustained and controlled release system for GET (Glycosaminoglycan-enhanced transducing)-tagged proteins using S/O/W PLGA microparticle fabrication. We demonstrated that PLGA and its acidic degradants inhibit GET-mediated transduction, which can be overcome by using pH-activated l-Histidine. l-Histidine inhibits the electrostatic interaction of GET/PLGA and allows enhanced intracellular transduction. GET could provide a powerful tool to program cell behaviour either in gradients or with sustained delivery. We believe that our controlled release systems will allow application of GET for tissue regeneration directly by TF cellular programming. [Mh] Termos MeSH primário: Ácido Láctico Peptídeos Ácido Poliglicólico Transdução Genética/métodos [Mh] Termos MeSH secundário: Animais Preparações de Ação Retardada/síntese química Preparações de Ação Retardada/química Preparações de Ação Retardada/farmacologia Ácido Láctico/química Ácido Láctico/farmacologia Camundongos Células NIH 3T3 Peptídeos/síntese química Peptídeos/química Peptídeos/farmacologia Ácido Poliglicólico/química Ácido Poliglicólico/farmacologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Delayed-Action Preparations); 0 (Peptides); 0 (polylactic acid-polyglycolic acid copolymer); 26009-03-0 (Polyglycolic Acid); 33X04XA5AT (Lactic Acid) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180223 [Lr] Data última revisão: 180223 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170502 [St] Status: MEDLINE

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[PMID]: 29216591 [Au] Autor: Sun Y; Jensen H; Petersen NJ; Larsen SW; Østergaard J [Ad] Endereço: Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100, Copenhagen, Denmark. Electronic address: yu.sun@sund.ku.dk. [Ti] Título: Concomitant monitoring of implant formation and drug release of in situ forming poly (lactide-co-glycolide acid) implants in a hydrogel matrix mimicking the subcutis using UV-vis imaging. [So] Source: J Pharm Biomed Anal;150:95-106, 2018 Feb 20. [Is] ISSN: 1873-264X [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: For poly (lactide-co-glycolide acid) (PLGA)-based in situ forming implants, the rate of implant formation plays an important role in determining the overall drug release kinetics. Currently, in vitro techniques capable of characterizing the processes of drug release and implant formation at the same time are not available. A hydrogel-based in vitro experimental setup was recently developed requiring only microliter of formulation and forming a closed system potentially suitable for interfacing with various spectroscopic techniques. The aim of the present proof-of-concept study was to investigate the feasibility of concomitant UV imaging, Vis imaging and light microscopy for detailed characterization of the behavior of in situ forming PLGA implants in the hydrogel matrix mimicking the subcutis. The model compounds, piroxicam and α-lactalbumin were added to PLGA-1-methyl-2-pyrrolidinone and PLGA-triacetin solutions. Upon bringing the PLGA-solvent-compound pre-formulation in contact with the hydrogel, Vis imaging and light microscopy were applied to visualize the depot formation and UV imaging was used to quantify drug transport in the hydrogel. As compared to piroxicam, the α-lactalbumin invoked an acceleration of phase separation and an increase of implant size. α-Lactalbumin was released faster from the PLGA-1-methyl-2-pyrrolidinone system than the PLGA-triacetin system opposite to the piroxicam release pattern. A linear relationship between the rate of implant formation and initial compound release within the first 4h was established for the PLGA-NMP systems. This implies that phase separation may be one of the controlling factors in drug release. The rate of implant formation may be an important parameter for predicting and tailoring drug release. The approach combining UV imaging, Vis imaging and light microscopy may facilitate understanding of release processes and holds potential for becoming a useful tool in formulation development of in situ forming implants. [Mh] Termos MeSH primário: Sistemas de Liberação de Medicamentos Lactalbumina/administração & dosagem Ácido Láctico/química Piroxicam/administração & dosagem Ácido Poliglicólico/química [Mh] Termos MeSH secundário: Química Farmacêutica/métodos Portadores de Fármacos/química Implantes de Medicamento Liberação Controlada de Fármacos Hidrogéis Pirrolidinonas/química Espectrofotometria Ultravioleta/métodos Análise Espectral/métodos Tela Subcutânea/metabolismo Triacetina/química [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Drug Carriers); 0 (Drug Implants); 0 (Hydrogels); 0 (Pyrrolidinones); 0 (polylactic acid-polyglycolic acid copolymer); 13T4O6VMAM (Piroxicam); 26009-03-0 (Polyglycolic Acid); 33X04XA5AT (Lactic Acid); 9013-90-5 (Lactalbumin); JR9CE63FPM (N-methylpyrrolidone); XHX3C3X673 (Triacetin) [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180130 [Lr] Data última revisão: 180130 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171208 [St] Status: MEDLINE

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[PMID]: 29172759 [Au] Autor: Wang S; Shao M; Zhong Z; Wang A; Cao J; Lu Y; Wang Y; Zhang J [Ad] Endereço: a State Key Laboratory of Quality Research in Chinese Medicine , Institute of Chinese Medical Sciences, University of Macau , Macau , China. [Ti] Título: Co-delivery of gambogic acid and TRAIL plasmid by hyaluronic acid grafted PEI-PLGA nanoparticles for the treatment of triple negative breast cancer. [So] Source: Drug Deliv;24(1):1791-1800, 2017 Nov. [Is] ISSN: 1521-0464 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-based combination therapy and gene therapy are new strategies to potentially overcome the limitations of TRAIL, however, the lack of efficient and low toxic vectors remains the major obstacle. In this study, we developed a hyaluronic acid (HA)-decorated polyethylenimine-poly(d,l-lactide-co-glycolide) (PEI-PLGA) nanoparticle (NP) system for targeted co-delivery of TRAIL plasmid (pTRAIL) and gambogic acid (GA) in triple-negative breast cancer (TNBC) therapy. GA was encapsulated into the core of the PEI-PLGA NPs while pTRAIL was adsorbed onto the positive NP surface via charge adsorption. The coating of HA on PEI-PLGA NPs functions as a targeting ligand by binding to CD44 receptor of TNBC cells and a shell to neutralize the excess positive charge of inner NPs. The resultant pTRAIL and GA co-loaded HA-coated PEI-PLGA NPs exhibited spherical shape (121.5 nm) and could promote the internalization of loaded cargoes into TNBC cells through the CD44-dependent endocytic pathway. The dual drug-loaded NPs significantly augmented apoptotic cell death in vitro and inhibited TNBC tumor growth in vivo. This multifunctional NP system efficiently co-delivered GA and pTRAIL, thus representing a promising strategy to treat TNBC and bringing forth a platform strategy for co-delivery of therapeutic DNA and chemotherapeutic agents in combinatorial TNBC therapy. [Mh] Termos MeSH primário: Ácido Hialurônico/administração & dosagem Ácido Láctico/administração & dosagem Nanopartículas/administração & dosagem Plasmídeos/administração & dosagem Polietilenoimina/administração & dosagem Ácido Poliglicólico/administração & dosagem Ligante Indutor de Apoptose Relacionado a TNF/administração & dosagem Neoplasias de Mama Triplo Negativas/tratamento farmacológico Xantonas/administração & dosagem [Mh] Termos MeSH secundário: Animais Antineoplásicos/administração & dosagem Linhagem Celular Tumoral Portadores de Fármacos/administração & dosagem Seres Humanos Receptores de Hialuronatos/metabolismo Células MCF-7 Camundongos Neoplasias de Mama Triplo Negativas/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antineoplastic Agents); 0 (Drug Carriers); 0 (Hyaluronan Receptors); 0 (TNF-Related Apoptosis-Inducing Ligand); 0 (Xanthones); 0 (polylactic acid-polyglycolic acid copolymer); 26009-03-0 (Polyglycolic Acid); 33X04XA5AT (Lactic Acid); 8N585K83U2 (gambogic acid); 9002-98-6 (Polyethyleneimine); 9004-61-9 (Hyaluronic Acid) [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180130 [Lr] Data última revisão: 180130 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171128 [St] Status: MEDLINE [do] DOI: 10.1080/10717544.2017.1406558

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[PMID]: 28851247 [Au] Autor: Dai J; Long W; Liang Z; Wen L; Yang F; Chen G [Ad] Endereço: a School of Pharmacy , Guangdong Pharmaceutical University , Guangzhou , China. [Ti] Título: A novel vehicle for local protein delivery to the inner ear: injectable and biodegradable thermosensitive hydrogel loaded with PLGA nanoparticles. [So] Source: Drug Dev Ind Pharm;44(1):89-98, 2018 Jan. [Is] ISSN: 1520-5762 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Delivery of biomacromolecular drugs into the inner ear is challenging, mainly because of their inherent instability as well as physiological and anatomical barriers. Therefore, protein-friendly, hydrogel-based delivery systems following local administration are being developed for inner ear therapy. Herein, biodegradable poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) containing interferon α-2 b (IFN α-2 b) were loaded in chitosan/glycerophosphate (CS/GP)-based thermosensitive hydrogel for IFN delivery by intratympanic injection. The injectable hydrogel possessed a physiological pH and formed semi-solid gel at 37 °C, with good swelling and deswelling properties. The CS/GP hydrogel could slowly degrade as visualized by scanning electron microscopy (SEM). The presence of NPs in CS/GP gel largely influenced in vitro drug release. In the guinea pig cochlea, a 1.5- to 3-fold increase in the drug exposure time of NPs-CS/GP was found than those of the solution, NPs and IFN-loaded hydrogel. Most importantly, a prolonged residence time was attained without obvious histological changes in the inner ear. This biodegradable, injectable, and thermosensitive NPs-CS/GP system may allow longer delivery of protein drugs to the inner ear, thus may be a potential novel vehicle for inner ear therapy. [Mh] Termos MeSH primário: Quitosana/química Orelha Interna/fisiologia Excipientes/química Glicerofosfatos/química Hidrogel de Polietilenoglicol-Dimetacrilato/química Interferon-alfa/química Ácido Láctico/química Nanopartículas/química Ácido Poliglicólico/química [Mh] Termos MeSH secundário: Animais Sistemas de Liberação de Medicamentos Cobaias Proteínas Recombinantes/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Excipients); 0 (Glycerophosphates); 0 (Interferon-alpha); 0 (Recombinant Proteins); 0 (polylactic acid-polyglycolic acid copolymer); 25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate); 26009-03-0 (Polyglycolic Acid); 33X04XA5AT (Lactic Acid); 43K1W2T1M6 (interferon alfa-2b); 9012-76-4 (Chitosan) [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180111 [Lr] Data última revisão: 180111 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170831 [St] Status: MEDLINE [do] DOI: 10.1080/03639045.2017.1373803

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[PMID]: 28449614 [Au] Autor: Encinas-Basurto D; Ibarra J; Juarez J; Burboa MG; Barbosa S; Taboada P; Troncoso-Rojas R; Valdez MA [Ad] Endereço: a Departamento de Física , Posgrado en Nanotecnología, Universidad de Sonora, Rosales y Transversal , Hermosillo , Sonora , México. [Ti] Título: Poly(lactic-co-glycolic acid) nanoparticles for sustained release of allyl isothiocyanate: characterization, in vitro release and biological activity. [So] Source: J Microencapsul;34(3):231-242, 2017 May. [Is] ISSN: 1464-5246 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The objective of this study is to establish the ability of entrap allyl isothiocyanate (AITC) into polymeric nanoparticles to extend its shelf life and enhance its antiproliferative properties. Natural compounds, such as AITC, have showed multi-targeting activity resulting in a wide-range spectrum of therapeutic properties in chronic and degenerative diseases, conversely with most current pharmaceutical drugs showing single targeting activity and often result in drug resistance after extended administration periods. Apparently, AITC-loaded poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) reduced AITC degradation and volatility and were able to extend AITC shelf life compared with free AITC (65% vs. 20% in 24 h, respectively). Cell viability and uptake of AITC-loaded nanoparticles were studied in vitro, showing that the protection and sustained release of AITC from polymeric NPs involved a larger toxicity of tumoral cells. These nanoparticles could be used as protective systems for enhancing a biological activity. [Mh] Termos MeSH primário: Preparações de Ação Retardada Portadores de Fármacos/química Isotiocianatos/administração & dosagem Ácido Láctico/química Nanopartículas/química Ácido Poliglicólico/química [Mh] Termos MeSH secundário: Linhagem Celular Tumoral Seres Humanos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Delayed-Action Preparations); 0 (Drug Carriers); 0 (Isothiocyanates); 0 (polylactic acid-polyglycolic acid copolymer); 26009-03-0 (Polyglycolic Acid); 33X04XA5AT (Lactic Acid); BN34FX42G3 (allyl isothiocyanate) [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180105 [Lr] Data última revisão: 180105 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170429 [St] Status: MEDLINE [do] DOI: 10.1080/02652048.2017.1323037

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[PMID]: 29186202 [Au] Autor: Fu C; Bai H; Zhu J; Niu Z; Wang Y; Li J; Yang X; Bai Y [Ad] Endereço: Department of Orthopedic Surgery, the Second Hospital of Jilin University, Changchun, Jilin, P. R. China. [Ti] Título: Enhanced cell proliferation and osteogenic differentiation in electrospun PLGA/hydroxyapatite nanofibre scaffolds incorporated with graphene oxide. [So] Source: PLoS One;12(11):e0188352, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: One of the goals of bone tissue engineering is to mimic native ECM in architecture and function, creating scaffolds with excellent biocompatibility, osteoinductive ability and mechanical properties. The aim of this study was to fabricate nanofibrous matrices by electrospinning a blend of poly (L-lactic-co-glycolic acid) (PLGA), hydroxyapatite (HA), and grapheme oxide (GO) as a favourable platform for bone tissue engineering. The morphology, biocompatibility, mechanical properties, and biological activity of all nanofibrous matrices were compared. The data indicate that the hydrophilicity and protein adsorption rate of the fabricated matrices were significantly increased by blending with a small amount of HA and GO. Furthermore, GO significantly boosted the tensile strength of the nanofibrous matrices, and the PLGA/GO/HA nanofibrous matrices can serve as mechanically stable scaffolds for cell growth. For further test in vitro, MC3T3-E1 cells were cultured on the PLGA/HA/GO nanofbrous matrices to observe various cellular activities and cell mineralization. The results indicated that the PLGA/GO/HA nanofibrous matrices significantly enhanced adhesion, and proliferation in MCET3-E1 cells and functionally promoted alkaline phosphatase (ALP) activity, the osteogenesis-related gene expression and mineral deposition. Therefore, the PLGA/HA/GO composite nanofibres are excellent and versatile scaffolds for applications in bone tissue regeneration. [Mh] Termos MeSH primário: Diferenciação Celular Proliferação Celular Durapatita Grafite/química Ácido Láctico Nanofibras Osteogênese/fisiologia Ácido Poliglicólico Tecidos Suporte [Mh] Termos MeSH secundário: Células 3T3 Adsorção Animais Camundongos Resistência à Tração [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (polylactic acid-polyglycolic acid copolymer); 26009-03-0 (Polyglycolic Acid); 33X04XA5AT (Lactic Acid); 7782-42-5 (Graphite); 91D9GV0Z28 (Durapatite) [Em] Mês de entrada: 1712 [Cu] Atualização por classe: 171226 [Lr] Data última revisão: 171226 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171130 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0188352

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MEDLINE

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[PMID]: 28454494 [Au] Autor: Qu S; Zhao L; Zhu J; Wang C; Dai C; Guo H; Hao Z [Ad] Endereço: a Agricultural Bio-Pharmaceutical Laboratory, Qingdao Agricultural University , Qingdao , China and. [Ti] Título: Preparation and testing of cefquinome-loaded poly lactic-co-glycolic acid microspheres for lung targeting. [So] Source: Drug Deliv;24(1):745-751, 2017 Nov. [Is] ISSN: 1521-0464 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The aim of this study was to prepare cefquinome-loaded poly lactic-co-glycolic acid (PLGA) microspheres and to evaluate their in vitro and in vivo characteristics. Microspheres were prepared using a spry drier and were characterized in terms of morphology, size, drug-loading coefficient, encapsulation ratio and in vitro release. The prepared microspheres were spherical with smooth surfaces and uniform size (12.4 ± 1.2 µm). The encapsulation efficiency and drug loading of cefquinome was 91.6 ± 2.6 and 18.3 ± 1.3%, respectively. In vitro release of cefquinome from the microspheres was sustained for 36 h. In vivo studies identified the lung as the target tissue and the region of maximum cefquinome release. A partial lung inflammation was observed but disappeared spontaneously as the microspheres were removed through in vivo decay. The sustained cefquinome release from the microspheres revealed its applicability as a drug delivery system that minimized exposure to healthy tissues while increasing the accumulation of therapeutic drug at the target site. These results indicated that the spray-drying method of loading cefquinome into PLGA microspheres is a straightforward method for lung targeting in animals. [Mh] Termos MeSH primário: Microesferas [Mh] Termos MeSH secundário: Animais Cefalosporinas Glicóis Ácido Láctico Pulmão Tamanho da Partícula Ácido Poliglicólico [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Cephalosporins); 0 (Glycols); 0 (polylactic acid-polyglycolic acid copolymer); 26009-03-0 (Polyglycolic Acid); 33X04XA5AT (Lactic Acid); Z74S078CWP (cefquinome) [Em] Mês de entrada: 1712 [Cu] Atualização por classe: 171212 [Lr] Data última revisão: 171212 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170430 [St] Status: MEDLINE [do] DOI: 10.1080/10717544.2017.1321058

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