[PMID]: | 29253565 |
[Au] Autor: | Lee W; Ko KR; Kim HK; Lim S; Kim S |
[Ad] Endereço: | Department of Biological Sciences, Seoul National University, Seoul 151-742, South Korea; ViroMed Co., Ltd., Seoul 151-747, South Korea. |
[Ti] Título: | Dehydrodiconiferyl alcohol promotes BMP-2-induced osteoblastogenesis through its agonistic effects on estrogen receptor. |
[So] Source: | Biochem Biophys Res Commun;495(3):2242-2248, 2018 01 15. |
[Is] ISSN: | 1090-2104 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | Estrogen deficiency results in an imbalance between the levels of bone-resorping osteoclasts and bone-forming osteoblasts, eventually leading to overall bone loss. Dehydrodiconiferyl alcohol (DHCA), a lignan compound originally isolated from Cucurbita moschata, has been shown to bind to estrogen receptor, and indeed exhibits various activities of estrogen, such as anti-inflammatory and anti-oxidative stress effects. In this study, we tested whether synthetic DHCA could affect the BMP-2-induced osteoblastogenesis in vitro. In MC3T3-E1 cells, DHCA promoted BMP-2-induced differentiation of osteoblasts. Consistently, the expression of three osteoblastogenic genes known to be induced by BMP-2, ALP, osteocalcin and OPG, was up-regulated by DHCA treatment. DHCA was also shown to activate the production of RUNX2 by activating Smad1/5/9 and AMPK. Data from transient transfection assays suggested that DHCA might activate the estrogen receptor signaling pathway. Effects of DHCA on BMP-2-induced osteoblastogenesis were reduced when cells were treated with a specific siRNA to ERα or ERß. Taken together, our results suggest that DHCA may be developed as an efficient therapeutic for osteoporosis by regulating osteoblastogenesis through its estrogenic effects. |
[Mh] Termos MeSH primário: |
Proteína Morfogenética Óssea 2/metabolismo Moduladores de Receptor Estrogênico/administração & dosagem Estrogênios/metabolismo Osteoblastos/fisiologia Osteogênese/fisiologia Fenóis/administração & dosagem Receptores Estrogênicos/metabolismo
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[Mh] Termos MeSH secundário: |
Células 3T3 Animais Diferenciação Celular/efeitos dos fármacos Diferenciação Celular/fisiologia Relação Dose-Resposta a Droga Camundongos Osteoblastos/citologia Osteoblastos/efeitos dos fármacos Osteogênese/efeitos dos fármacos Receptores Estrogênicos/efeitos dos fármacos
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[Pt] Tipo de publicação: | JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T |
[Nm] Nome de substância:
| 0 (Bmp2 protein, mouse); 0 (Bone Morphogenetic Protein 2); 0 (Estrogen Receptor Modulators); 0 (Estrogens); 0 (Phenols); 0 (Receptors, Estrogen); 4263-87-0 (dehydrodiconiferyl alcohol) |
[Em] Mês de entrada: | 1802 |
[Cu] Atualização por classe: | 180214 |
[Lr] Data última revisão:
| 180214 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 171219 |
[St] Status: | MEDLINE |
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